J H Exton

Summary

Affiliation: Vanderbilt University
Country: USA

Publications

  1. ncbi request reprint Regulation of phospholipase D
    J H Exton
    Howard Hughes Medical Institute and Department of Molecular Physiology and Biophysics Vanderbilt University School of Medicine, Nashville, TN 37232 0295, USA
    Biochim Biophys Acta 1439:121-33. 1999
  2. ncbi request reprint Arfophilin is a common target of both class II and class III ADP-ribosylation factors
    O H Shin
    Howard Hughes Medical Institute and Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    Biochemistry 40:10846-52. 2001
  3. ncbi request reprint Differential binding of arfaptin 2/POR1 to ADP-ribosylation factors and Rac1
    O H Shin
    Howard Hughes Medical Institute, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0295, USA
    Biochem Biophys Res Commun 285:1267-73. 2001
  4. ncbi request reprint Arfaptin 1 forms a complex with ADP-ribosylation factor and inhibits phospholipase D
    B T Williger
    Howard Hughes Medical Institute and Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, TN 37232 0295, USA
    FEBS Lett 454:85-9. 1999
  5. ncbi request reprint Ca2+/calmodulin-dependent protein kinase II regulates Tiam1 by reversible protein phosphorylation
    I N Fleming
    Howard Hughes Medical Institute and the Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0295, USA
    J Biol Chem 274:12753-8. 1999
  6. ncbi request reprint Identification of arfophilin, a target protein for GTP-bound class II ADP-ribosylation factors
    O H Shin
    Howard Hughes Medical Institute and Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    J Biol Chem 274:36609-15. 1999
  7. ncbi request reprint Analysis of platelet-derived growth factor-induced phospholipase D activation in mouse embryo fibroblasts lacking phospholipase C-gamma1
    J A Hess
    Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0295, USA
    J Biol Chem 273:20517-24. 1998
  8. ncbi request reprint Cloning and characterization of phospholipase D from rat brain
    S K Park
    Howard Hughes Medical Institute and Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0295, USA
    J Biol Chem 272:29263-71. 1997
  9. pmc Determination of interaction sites of phospholipase D1 for RhoA
    S Cai
    Howard Hughes Medical Institute, Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, TN 37232 0295, USA
    Biochem J 355:779-85. 2001
  10. ncbi request reprint Requirements and effects of palmitoylation of rat PLD1
    Z Xie
    Howard Hughes Medical Institute and Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0295, USA
    J Biol Chem 276:9383-91. 2001

Collaborators

Detail Information

Publications17

  1. ncbi request reprint Regulation of phospholipase D
    J H Exton
    Howard Hughes Medical Institute and Department of Molecular Physiology and Biophysics Vanderbilt University School of Medicine, Nashville, TN 37232 0295, USA
    Biochim Biophys Acta 1439:121-33. 1999
    ..PLD1 undergoes tyrosine phosphorylation in response to H(2)O(2) treatment of cells. The functional consequence of this phosphorylation and soluble tyrosine kinase(s) involved are presently unknown...
  2. ncbi request reprint Arfophilin is a common target of both class II and class III ADP-ribosylation factors
    O H Shin
    Howard Hughes Medical Institute and Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    Biochemistry 40:10846-52. 2001
    ..Our findings suggest that both class II and III ARFs may influence the same cellular pathways through arfophilin as a common downstream effector...
  3. ncbi request reprint Differential binding of arfaptin 2/POR1 to ADP-ribosylation factors and Rac1
    O H Shin
    Howard Hughes Medical Institute, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0295, USA
    Biochem Biophys Res Commun 285:1267-73. 2001
    ..Based on these observations, we suggest that arfaptin 2/POR1 is a target protein for GTP-ARFs and for GDP-Rac1, and that it may be involved in interactions between the Rac and ARF signaling pathways...
  4. ncbi request reprint Arfaptin 1 forms a complex with ADP-ribosylation factor and inhibits phospholipase D
    B T Williger
    Howard Hughes Medical Institute and Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, TN 37232 0295, USA
    FEBS Lett 454:85-9. 1999
    ..These two domains are needed for arfaptin 1 inhibition of PLD activation by myrARF3 in vitro...
  5. ncbi request reprint Ca2+/calmodulin-dependent protein kinase II regulates Tiam1 by reversible protein phosphorylation
    I N Fleming
    Howard Hughes Medical Institute and the Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0295, USA
    J Biol Chem 274:12753-8. 1999
    ..These data demonstrate that protein kinase Calpha and Ca2+/calmodulin-dependent protein kinase II phosphorylate Tiam1 in vivo, and that the latter kinase plays a key role in regulating the activity of this exchange factor in vitro...
  6. ncbi request reprint Identification of arfophilin, a target protein for GTP-bound class II ADP-ribosylation factors
    O H Shin
    Howard Hughes Medical Institute and Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    J Biol Chem 274:36609-15. 1999
    ..ARF5 containing the N terminus of ARF3 did not promote translocation indicating that class II ARFs are specific carriers for arfophilin...
  7. ncbi request reprint Analysis of platelet-derived growth factor-induced phospholipase D activation in mouse embryo fibroblasts lacking phospholipase C-gamma1
    J A Hess
    Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0295, USA
    J Biol Chem 273:20517-24. 1998
    ..In summary, these data indicate that PLC-gamma is required for growth factor-induced activation of PLD in MEFs...
  8. ncbi request reprint Cloning and characterization of phospholipase D from rat brain
    S K Park
    Howard Hughes Medical Institute and Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0295, USA
    J Biol Chem 272:29263-71. 1997
    ..In addition, the effects of phorbol ester are much greater in the intact cells. It is concluded that the regulation of rPLD in intact COS-7 cells differs significantly from that seen in vitro; possible reasons for this are discussed...
  9. pmc Determination of interaction sites of phospholipase D1 for RhoA
    S Cai
    Howard Hughes Medical Institute, Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, TN 37232 0295, USA
    Biochem J 355:779-85. 2001
    ..It is concluded that basic amino acids in a restricted C-terminal region of rPLD(1) are important for binding of RhoA and its activation of PLD activity...
  10. ncbi request reprint Requirements and effects of palmitoylation of rat PLD1
    Z Xie
    Howard Hughes Medical Institute and Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0295, USA
    J Biol Chem 276:9383-91. 2001
    ..Mutations of cysteines 240 and 241 to alanine abolish the extensive Ser/Thr phosphorylation of the enzyme and weaken its association with membranes...
  11. pmc Phospholipase D activity is required for actin stress fiber formation in fibroblasts
    Y Kam
    Howard Hughes Medical Institute and Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    Mol Cell Biol 21:4055-66. 2001
    ..These results indicate that PLD activity is required for LPA-induced rearrangement of the actin cytoskeleton to form stress fibers and that PLD might be involved in the cross-linking of actin filaments mediated by alpha-actinin...
  12. ncbi request reprint Determination of interaction sites on the small G protein RhoA for phospholipase D
    C D Bae
    Howard Hughes Medical Institute and the Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    J Biol Chem 273:11596-604. 1998
    ....
  13. ncbi request reprint Activation of RhoA by association of Galpha(13) with Dbl
    S Jin
    Howard Hughes Medical Institute and Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee, 37232 0295, USA
    Biochem Biophys Res Commun 277:718-21. 2000
    ..These findings illustrate that activated Galpha(13) and Dbl can associate in vivo and that this leads to Rho activation...
  14. ncbi request reprint Phospholipase D-structure, regulation and function
    J H Exton
    Howard Hughes Medical Institute, Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, TN, USA
    Rev Physiol Biochem Pharmacol 144:1-94. 2002
  15. ncbi request reprint Phospholipase C-gamma, protein kinase C and Ca2+/calmodulin-dependent protein kinase II are involved in platelet-derived growth factor-induced phosphorylation of Tiam1
    I N Fleming
    Howard Hughes Medical Institute and Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    FEBS Lett 429:229-33. 1998
    ..Moreover, PDGF-stimulated Tiam1 phosphorylation was markedly reduced by 72 +/- 10% in PLC-gamma1 deficient mouse fibroblasts, compared to wild-type cells, indicating that phosphoinositide phospholipase C is involved...
  16. ncbi request reprint Phospholipase D
    J H Exton
    Howard Hughes Medical Institute, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0295, USA
    Ann N Y Acad Sci 905:61-8. 2000
    ..The cellular locations and specific functions of the two PLD isoforms remain to be established...
  17. ncbi request reprint Arfaptin 1, a putative cytosolic target protein of ADP-ribosylation factor, is recruited to Golgi membranes
    H Kanoh
    Howard Hughes Medical Institute and the Department of Molecular Physiology and Biophysics and Pharmacology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    J Biol Chem 272:5421-9. 1997
    ..These results suggest that arfaptins 1 and 2 may be direct target proteins of class 1 ARFs. Arfaptin 1 may be involved in Golgi function along with ARF1...