J H Exton

Summary

Affiliation: Vanderbilt University
Country: USA

Publications

  1. ncbi Regulation of phospholipase D
    J H Exton
    Howard Hughes Medical Institute and Department of Molecular Physiology and Biophysics Vanderbilt University School of Medicine, Nashville, TN 37232 0295, USA
    Biochim Biophys Acta 1439:121-33. 1999
  2. ncbi Arfophilin is a common target of both class II and class III ADP-ribosylation factors
    O H Shin
    Howard Hughes Medical Institute and Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    Biochemistry 40:10846-52. 2001
  3. ncbi Differential binding of arfaptin 2/POR1 to ADP-ribosylation factors and Rac1
    O H Shin
    Howard Hughes Medical Institute, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0295, USA
    Biochem Biophys Res Commun 285:1267-73. 2001
  4. ncbi Arfaptin 1 forms a complex with ADP-ribosylation factor and inhibits phospholipase D
    B T Williger
    Howard Hughes Medical Institute and Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, TN 37232 0295, USA
    FEBS Lett 454:85-9. 1999
  5. ncbi Ca2+/calmodulin-dependent protein kinase II regulates Tiam1 by reversible protein phosphorylation
    I N Fleming
    Howard Hughes Medical Institute and the Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0295, USA
    J Biol Chem 274:12753-8. 1999
  6. ncbi Identification of arfophilin, a target protein for GTP-bound class II ADP-ribosylation factors
    O H Shin
    Howard Hughes Medical Institute and Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    J Biol Chem 274:36609-15. 1999
  7. ncbi Analysis of platelet-derived growth factor-induced phospholipase D activation in mouse embryo fibroblasts lacking phospholipase C-gamma1
    J A Hess
    Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0295, USA
    J Biol Chem 273:20517-24. 1998
  8. ncbi Cloning and characterization of phospholipase D from rat brain
    S K Park
    Howard Hughes Medical Institute and Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0295, USA
    J Biol Chem 272:29263-71. 1997
  9. ncbi Determination of interaction sites of phospholipase D1 for RhoA
    S Cai
    Howard Hughes Medical Institute, Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, TN 37232 0295, USA
    Biochem J 355:779-85. 2001
  10. ncbi Requirements and effects of palmitoylation of rat PLD1
    Z Xie
    Howard Hughes Medical Institute and Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0295, USA
    J Biol Chem 276:9383-91. 2001

Collaborators

Detail Information

Publications17

  1. ncbi Regulation of phospholipase D
    J H Exton
    Howard Hughes Medical Institute and Department of Molecular Physiology and Biophysics Vanderbilt University School of Medicine, Nashville, TN 37232 0295, USA
    Biochim Biophys Acta 1439:121-33. 1999
    ..PLD1 undergoes tyrosine phosphorylation in response to H(2)O(2) treatment of cells. The functional consequence of this phosphorylation and soluble tyrosine kinase(s) involved are presently unknown...
  2. ncbi Arfophilin is a common target of both class II and class III ADP-ribosylation factors
    O H Shin
    Howard Hughes Medical Institute and Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    Biochemistry 40:10846-52. 2001
    ..Our findings suggest that both class II and III ARFs may influence the same cellular pathways through arfophilin as a common downstream effector...
  3. ncbi Differential binding of arfaptin 2/POR1 to ADP-ribosylation factors and Rac1
    O H Shin
    Howard Hughes Medical Institute, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0295, USA
    Biochem Biophys Res Commun 285:1267-73. 2001
    ..Based on these observations, we suggest that arfaptin 2/POR1 is a target protein for GTP-ARFs and for GDP-Rac1, and that it may be involved in interactions between the Rac and ARF signaling pathways...
  4. ncbi Arfaptin 1 forms a complex with ADP-ribosylation factor and inhibits phospholipase D
    B T Williger
    Howard Hughes Medical Institute and Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, TN 37232 0295, USA
    FEBS Lett 454:85-9. 1999
    ..These two domains are needed for arfaptin 1 inhibition of PLD activation by myrARF3 in vitro...
  5. ncbi Ca2+/calmodulin-dependent protein kinase II regulates Tiam1 by reversible protein phosphorylation
    I N Fleming
    Howard Hughes Medical Institute and the Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0295, USA
    J Biol Chem 274:12753-8. 1999
    ..These data demonstrate that protein kinase Calpha and Ca2+/calmodulin-dependent protein kinase II phosphorylate Tiam1 in vivo, and that the latter kinase plays a key role in regulating the activity of this exchange factor in vitro...
  6. ncbi Identification of arfophilin, a target protein for GTP-bound class II ADP-ribosylation factors
    O H Shin
    Howard Hughes Medical Institute and Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    J Biol Chem 274:36609-15. 1999
    ..ARF5 containing the N terminus of ARF3 did not promote translocation indicating that class II ARFs are specific carriers for arfophilin...
  7. ncbi Analysis of platelet-derived growth factor-induced phospholipase D activation in mouse embryo fibroblasts lacking phospholipase C-gamma1
    J A Hess
    Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0295, USA
    J Biol Chem 273:20517-24. 1998
    ..In summary, these data indicate that PLC-gamma is required for growth factor-induced activation of PLD in MEFs...
  8. ncbi Cloning and characterization of phospholipase D from rat brain
    S K Park
    Howard Hughes Medical Institute and Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0295, USA
    J Biol Chem 272:29263-71. 1997
    ..In addition, the effects of phorbol ester are much greater in the intact cells. It is concluded that the regulation of rPLD in intact COS-7 cells differs significantly from that seen in vitro; possible reasons for this are discussed...
  9. ncbi Determination of interaction sites of phospholipase D1 for RhoA
    S Cai
    Howard Hughes Medical Institute, Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, TN 37232 0295, USA
    Biochem J 355:779-85. 2001
    ..It is concluded that basic amino acids in a restricted C-terminal region of rPLD(1) are important for binding of RhoA and its activation of PLD activity...
  10. ncbi Requirements and effects of palmitoylation of rat PLD1
    Z Xie
    Howard Hughes Medical Institute and Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0295, USA
    J Biol Chem 276:9383-91. 2001
    ..Mutations of cysteines 240 and 241 to alanine abolish the extensive Ser/Thr phosphorylation of the enzyme and weaken its association with membranes...
  11. ncbi Phospholipase D activity is required for actin stress fiber formation in fibroblasts
    Y Kam
    Howard Hughes Medical Institute and Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    Mol Cell Biol 21:4055-66. 2001
    ..These results indicate that PLD activity is required for LPA-induced rearrangement of the actin cytoskeleton to form stress fibers and that PLD might be involved in the cross-linking of actin filaments mediated by alpha-actinin...
  12. ncbi Determination of interaction sites on the small G protein RhoA for phospholipase D
    C D Bae
    Howard Hughes Medical Institute and the Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    J Biol Chem 273:11596-604. 1998
    ....
  13. ncbi Activation of RhoA by association of Galpha(13) with Dbl
    S Jin
    Howard Hughes Medical Institute and Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee, 37232 0295, USA
    Biochem Biophys Res Commun 277:718-21. 2000
    ..These findings illustrate that activated Galpha(13) and Dbl can associate in vivo and that this leads to Rho activation...
  14. ncbi Phospholipase D-structure, regulation and function
    J H Exton
    Howard Hughes Medical Institute, Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, TN, USA
    Rev Physiol Biochem Pharmacol 144:1-94. 2002
  15. ncbi Phospholipase C-gamma, protein kinase C and Ca2+/calmodulin-dependent protein kinase II are involved in platelet-derived growth factor-induced phosphorylation of Tiam1
    I N Fleming
    Howard Hughes Medical Institute and Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    FEBS Lett 429:229-33. 1998
    ..Moreover, PDGF-stimulated Tiam1 phosphorylation was markedly reduced by 72 +/- 10% in PLC-gamma1 deficient mouse fibroblasts, compared to wild-type cells, indicating that phosphoinositide phospholipase C is involved...
  16. ncbi Phospholipase D
    J H Exton
    Howard Hughes Medical Institute, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0295, USA
    Ann N Y Acad Sci 905:61-8. 2000
    ..The cellular locations and specific functions of the two PLD isoforms remain to be established...
  17. ncbi Arfaptin 1, a putative cytosolic target protein of ADP-ribosylation factor, is recruited to Golgi membranes
    H Kanoh
    Howard Hughes Medical Institute and the Department of Molecular Physiology and Biophysics and Pharmacology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    J Biol Chem 272:5421-9. 1997
    ..These results suggest that arfaptins 1 and 2 may be direct target proteins of class 1 ARFs. Arfaptin 1 may be involved in Golgi function along with ARF1...