Niels de Jonge

Summary

Affiliation: Vanderbilt University
Country: USA

Publications

  1. pmc Electron microscopy of whole cells in liquid with nanometer resolution
    N de Jonge
    Department of Molecular Physiology and Biophysics, Vanderbilt University Medical Center, Nashville, TN 37232 0615, USA
    Proc Natl Acad Sci U S A 106:2159-64. 2009
  2. pmc Visualizing gold nanoparticle uptake in live cells with liquid scanning transmission electron microscopy
    Diana B Peckys
    Department of Molecular Physiology and Biophysics, Vanderbilt University Medical Center, Nashville, Tennessee 37232, United States
    Nano Lett 11:1733-8. 2011
  3. pmc Nanoscale imaging of whole cells using a liquid enclosure and a scanning transmission electron microscope
    Diana B Peckys
    Center for Environmental Biotechnology, University of Tennessee, Knoxville, Tennessee, United States of America
    PLoS ONE 4:e8214. 2009
  4. pmc Three-dimensional scanning transmission electron microscopy of biological specimens
    Niels de Jonge
    Vanderbilt University Medical Center, Department of Molecular Physiology and Biophysics, Light Hall 702, Nashville, TN 37232 0615, USA
    Microsc Microanal 16:54-63. 2010
  5. doi request reprint Atmospheric pressure scanning transmission electron microscopy
    Niels de Jonge
    Materials Science and Technology Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831, USA
    Nano Lett 10:1028-31. 2010
  6. pmc Nanometer-resolution electron microscopy through micrometers-thick water layers
    Niels de Jonge
    Vanderbilt University Medical Center, Department of Molecular Physiology and Biophysics, Nashville, TN 37232 0615, USA
    Ultramicroscopy 110:1114-9. 2010
  7. pmc Three-dimensional locations of gold-labeled proteins in a whole mount eukaryotic cell obtained with 3nm precision using aberration-corrected scanning transmission electron microscopy
    Madeline J Dukes
    Department of Chemistry, Vanderbilt University, Nashville, TN 37235, USA
    J Struct Biol 174:552-62. 2011
  8. doi request reprint Chromatic aberration-corrected tilt series transmission electron microscopy of nanoparticles in a whole mount macrophage cell
    Jean Pierre Baudoin
    Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, TN 37232 0615, USA
    Microsc Microanal 19:814-20. 2013
  9. pmc Correlative fluorescence microscopy and scanning transmission electron microscopy of quantum-dot-labeled proteins in whole cells in liquid
    Madeline J Dukes
    Department of Chemistry, Vanderbilt University, Nashville, Tennessee 37235, USA
    ACS Nano 4:4110-6. 2010
  10. pmc Whole-cell analysis of low-density lipoprotein uptake by macrophages using STEM tomography
    Jean Pierre Baudoin
    Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, TN, USA
    PLoS ONE 8:e55022. 2013

Collaborators

Detail Information

Publications15

  1. pmc Electron microscopy of whole cells in liquid with nanometer resolution
    N de Jonge
    Department of Molecular Physiology and Biophysics, Vanderbilt University Medical Center, Nashville, TN 37232 0615, USA
    Proc Natl Acad Sci U S A 106:2159-64. 2009
    ..The experimental findings are consistent with a theoretical calculation. Liquid STEM is a unique approach for imaging single molecules in whole cells with significantly improved resolution and imaging speed over existing methods...
  2. pmc Visualizing gold nanoparticle uptake in live cells with liquid scanning transmission electron microscopy
    Diana B Peckys
    Department of Molecular Physiology and Biophysics, Vanderbilt University Medical Center, Nashville, Tennessee 37232, United States
    Nano Lett 11:1733-8. 2011
    ..The vesicles accumulated to form a micrometer-sized cluster after 24 h of incubation. Two clusters were analyzed and found to consist of 117 ± 9 and 164 ± 4 NP-filled vesicles...
  3. pmc Nanoscale imaging of whole cells using a liquid enclosure and a scanning transmission electron microscope
    Diana B Peckys
    Center for Environmental Biotechnology, University of Tennessee, Knoxville, Tennessee, United States of America
    PLoS ONE 4:e8214. 2009
    ..Thirdly, the system is rather simple and involves only minimal new equipment in an electron microscopy (EM) laboratory...
  4. pmc Three-dimensional scanning transmission electron microscopy of biological specimens
    Niels de Jonge
    Vanderbilt University Medical Center, Department of Molecular Physiology and Biophysics, Light Hall 702, Nashville, TN 37232 0615, USA
    Microsc Microanal 16:54-63. 2010
    ..3D STEM images were also recorded of the Golgi ribbon in conventional thin sections containing 3T3 cells with a comparable axial resolution in the deconvolved dataset...
  5. doi request reprint Atmospheric pressure scanning transmission electron microscopy
    Niels de Jonge
    Materials Science and Technology Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831, USA
    Nano Lett 10:1028-31. 2010
    ..Gold nanoparticles of a full width at half-maximum diameter of 1.0 nm were visible above the background noise, and the achieved edge resolution was 0.4 nm in accordance with calculations of the beam broadening...
  6. pmc Nanometer-resolution electron microscopy through micrometers-thick water layers
    Niels de Jonge
    Vanderbilt University Medical Center, Department of Molecular Physiology and Biophysics, Nashville, TN 37232 0615, USA
    Ultramicroscopy 110:1114-9. 2010
    ..Applications of STEM imaging in liquid can be found in cell biology, e.g., to study tagged proteins in whole eukaryotic cells in liquid and in materials science to study the interaction of solid:liquid interfaces at the nanoscale...
  7. pmc Three-dimensional locations of gold-labeled proteins in a whole mount eukaryotic cell obtained with 3nm precision using aberration-corrected scanning transmission electron microscopy
    Madeline J Dukes
    Department of Chemistry, Vanderbilt University, Nashville, TN 37235, USA
    J Struct Biol 174:552-62. 2011
    ..The results of this study demonstrate the feasibility of using aberration-corrected STEM to study the 3D nanoparticle distribution in whole cells...
  8. doi request reprint Chromatic aberration-corrected tilt series transmission electron microscopy of nanoparticles in a whole mount macrophage cell
    Jean Pierre Baudoin
    Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, TN 37232 0615, USA
    Microsc Microanal 19:814-20. 2013
    ..The LDL is found to assemble in clusters. The clusters contain nanoparticles taken up on different days, which are joined without mixing their nanoparticle cargo. ..
  9. pmc Correlative fluorescence microscopy and scanning transmission electron microscopy of quantum-dot-labeled proteins in whole cells in liquid
    Madeline J Dukes
    Department of Chemistry, Vanderbilt University, Nashville, Tennessee 37235, USA
    ACS Nano 4:4110-6. 2010
    ..QDs of dimensions 7x12 nm were visible in a 5 microm thick layer of saline water, consistent with calculations. A spatial resolution of 3 nm was achieved on the QDs...
  10. pmc Whole-cell analysis of low-density lipoprotein uptake by macrophages using STEM tomography
    Jean Pierre Baudoin
    Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, TN, USA
    PLoS ONE 8:e55022. 2013
    ..Analysis of a 3D reconstruction revealed that newly up-taken LDL-gold was delivered to lysosomes containing previously up-taken LDL-gold thereby forming onion-like clusters...
  11. doi request reprint The influence of the sample thickness on the lateral and axial resolution of aberration-corrected scanning transmission electron microscopy
    Ranjan Ramachandra
    Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, TN 37232 0615, USA
    Microsc Microanal 19:93-101. 2013
    ..Beam broadening led to a reduced resolution for a 500-nm-thick membrane. The influence of the beam broadening on the axial resolution was also studied using Monte Carlo simulations with a 3D sample geometry...
  12. doi request reprint Electron microscopy of specimens in liquid
    Niels de Jonge
    Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, 2215 Garland Avenue, Nashville, Tennessee 37232, USA
    Nat Nanotechnol 6:695-704. 2011
    ..We conclude by assessing the potential role that electron microscopy of liquid samples can play in areas such as energy storage and bioimaging...
  13. pmc Fully hydrated yeast cells imaged with electron microscopy
    Diana B Peckys
    Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee, USA
    Biophys J 100:2522-9. 2011
    ..Achieving synergy between the capabilities of light and electron microscopy, we anticipate that liquid STEM will be broadly applied to explore the ultrastructure of live cells...
  14. pmc Optimized deconvolution for maximum axial resolution in three-dimensional aberration-corrected scanning transmission electron microscopy
    Ranjan Ramachandra
    Vanderbilt University School of Medicine, Department of Molecular Physiology and Biophysics, Nashville, TN 37232 0615, USA
    Microsc Microanal 18:218-28. 2012
    ..Thus, the deconvolution procedure optimized for the highest axial resolution is best suited for applications where one is interested in the 3D locations of nanoparticles only...
  15. ncbi request reprint End-specific strategies of attachment of long double stranded DNA onto gold-coated nanofiber arrays
    Diana B Peckys
    Materials Science and Technology Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831 6030, USA University of Tennessee in Knoxville, Knoxville, TN 37996 2200, USA
    Nanotechnology 19:435301. 2008
    ..Employing these site-specific and robust techniques of immobilization of dsDNA onto nanodevices can be of advantage for the study of DNA/protein interactions and for gene delivery applications...

Research Grants3

  1. Three dimensional aberration corrected scanning transmission electron microscopy
    Niels de Jonge; Fiscal Year: 2009
    ..Understanding how these structures are organized, and thereby function, within the crowded 3D volume of the cell can be applied, for example, to aid the development of new therapeutics, or improve existing ones. ..
  2. Three dimensional aberration corrected scanning transmission electron microscopy
    Niels de Jonge; Fiscal Year: 2010
    ..Understanding how these structures are organized, and thereby function, within the crowded 3D volume of the cell can be applied, for example, to aid the development of new therapeutics, or improve existing ones. ..