DAVID K contact CORTEZ

Summary

Affiliation: Vanderbilt University
Country: USA

Publications

  1. pmc Minichromosome maintenance proteins are direct targets of the ATM and ATR checkpoint kinases
    David Cortez
    Department of Biochemistry, Vanderbilt University, Nashville, TN 37232, USA
    Proc Natl Acad Sci U S A 101:10078-83. 2004
  2. pmc Unwind and slow down: checkpoint activation by helicase and polymerase uncoupling
    David Cortez
    Department of Biochemistry, Vanderbilt University, Nashville, TN 37232, USA
    Genes Dev 19:1007-12. 2005
  3. doi request reprint Phosphorylation of a C-terminal auto-inhibitory domain increases SMARCAL1 activity
    Clinton Carroll
    Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232 USA, Division of Pediatric Hematology Oncology, Vanderbilt University School of Medicine and Verna and Mars McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX, USA
    Nucleic Acids Res 42:918-25. 2014
  4. pmc Identification and characterization of SMARCAL1 protein complexes
    Remy Betous
    Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee, USA
    PLoS ONE 8:e63149. 2013
  5. ncbi request reprint Caffeine inhibits checkpoint responses without inhibiting the ataxia-telangiectasia-mutated (ATM) and ATM- and Rad3-related (ATR) protein kinases
    David Cortez
    Department of Biochemistry, Vanderbilt University, Nashville, Tennessee 37232, USA
    J Biol Chem 278:37139-45. 2003
  6. pmc Rapid activation of ATR by ionizing radiation requires ATM and Mre11
    Jeremy S Myers
    Department of Biochemistry, Vanderbilt University, Nashville, Tennessee 37232, USA
    J Biol Chem 281:9346-50. 2006
  7. pmc Function of the ATR N-terminal domain revealed by an ATM/ATR chimera
    Xinping Chen
    Department of Biochemistry, Vanderbilt University, Pierce Avenue, Nashville, TN 37232, USA
    Exp Cell Res 313:1667-74. 2007
  8. pmc ATRIP binding to replication protein A-single-stranded DNA promotes ATR-ATRIP localization but is dispensable for Chk1 phosphorylation
    Heather L Ball
    Department of Biochemistry, Vanderbilt University, Nashville, TN 37232, USA
    Mol Biol Cell 16:2372-81. 2005
  9. pmc Cyclin-dependent kinase 2 dependent phosphorylation of ATRIP regulates the G2-M checkpoint response to DNA damage
    Jeremy S Myers
    Department of Biochemistry and Mass Spectrometry Research Center, Vanderbilt University, 23rd Pierce Avenue, Nashville, TN 37232, USA
    Cancer Res 67:6685-90. 2007
  10. pmc DDB1 maintains genome integrity through regulation of Cdt1
    Courtney A Lovejoy
    Department of Biochemistry, Vanderbilt University, Nashville, TN 37232, USA
    Mol Cell Biol 26:7977-90. 2006

Research Grants

  1. Function of the ATR-ATRIP complex
    DAVID K contact CORTEZ; Fiscal Year: 2010
  2. Function of the ATR-ATRIP complex
    David Cortez; Fiscal Year: 2009
  3. Functional Analysis of Replication Stress Response Proteins
    David Cortez; Fiscal Year: 2009
  4. Function of the ATR-ATRIP complex
    David Cortez; Fiscal Year: 2009
  5. Function of the ATR-ATRIP complex
    David Cortez; Fiscal Year: 2007
  6. Signaling by the ATM and ATR Kinases
    David Cortez; Fiscal Year: 2006
  7. Functional Analysis of Replication Stress Response Proteins
    DAVID K CORTEZ; Fiscal Year: 2010

Collaborators

  • S J Elledge
  • Scott W Hiebert
  • Daniel A Mordes
  • Gloria G Glick
  • Courtney A Lovejoy
  • Runxiang Zhao
  • Heather L Ball
  • Carol E Bansbach
  • Jeremy S Myers
  • Xin Xu
  • Remy Betous
  • Edward A Nam
  • Clinton Carroll
  • Srividya Bhaskara
  • Walter J Chazin
  • Xinping Chen
  • Sung Yun Jung
  • Jun Qin
  • Bianca M Sirbu
  • Fei Ye
  • Yu Shyr
  • Laura C Titus
  • Brenda J Chyla
  • Sarah K Knutson
  • Zu Wen Sun
  • Joseph M Amann
  • Sivaraja Vaithiyalingam
  • Amy Joan L Ham
  • Mark R Ehrhardt
  • Ashwini Yenamandra
  • Kimberli Lock

Detail Information

Publications21

  1. pmc Minichromosome maintenance proteins are direct targets of the ATM and ATR checkpoint kinases
    David Cortez
    Department of Biochemistry, Vanderbilt University, Nashville, TN 37232, USA
    Proc Natl Acad Sci U S A 101:10078-83. 2004
    ..Thus, the MCM complex is a platform for multiple DNA damage-dependent regulatory signals that control DNA replication...
  2. pmc Unwind and slow down: checkpoint activation by helicase and polymerase uncoupling
    David Cortez
    Department of Biochemistry, Vanderbilt University, Nashville, TN 37232, USA
    Genes Dev 19:1007-12. 2005
  3. doi request reprint Phosphorylation of a C-terminal auto-inhibitory domain increases SMARCAL1 activity
    Clinton Carroll
    Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232 USA, Division of Pediatric Hematology Oncology, Vanderbilt University School of Medicine and Verna and Mars McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX, USA
    Nucleic Acids Res 42:918-25. 2014
    ..Thus, S889 phosphorylation is one mechanism by which SMARCAL1 activity is regulated to ensure the proper level of fork remodeling needed to maintain genome integrity during DNA synthesis. ..
  4. pmc Identification and characterization of SMARCAL1 protein complexes
    Remy Betous
    Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee, USA
    PLoS ONE 8:e63149. 2013
    ..These data suggest that RPA brings a complex of SMARCAL1 and WRN to stalled forks, but that they may act in different pathways to promote fork repair and restart...
  5. ncbi request reprint Caffeine inhibits checkpoint responses without inhibiting the ataxia-telangiectasia-mutated (ATM) and ATM- and Rad3-related (ATR) protein kinases
    David Cortez
    Department of Biochemistry, Vanderbilt University, Nashville, Tennessee 37232, USA
    J Biol Chem 278:37139-45. 2003
    ..This data suggests that although caffeine is an inhibitor of ATM-ATR kinase activity in vitro, it can block checkpoints without inhibiting ATM-ATR activation in vivo...
  6. pmc Rapid activation of ATR by ionizing radiation requires ATM and Mre11
    Jeremy S Myers
    Department of Biochemistry, Vanderbilt University, Nashville, Tennessee 37232, USA
    J Biol Chem 281:9346-50. 2006
    ..Thus, ATM and Mre11 may stimulate the ATR signaling pathway by converting DNA damage generated by IR into structures that recruit and activate ATR...
  7. pmc Function of the ATR N-terminal domain revealed by an ATM/ATR chimera
    Xinping Chen
    Department of Biochemistry, Vanderbilt University, Pierce Avenue, Nashville, TN 37232, USA
    Exp Cell Res 313:1667-74. 2007
    ..These data indicate that the N-terminus of ATR is sufficient to bind ATRIP and to promote localization to sites of replication stress...
  8. pmc ATRIP binding to replication protein A-single-stranded DNA promotes ATR-ATRIP localization but is dispensable for Chk1 phosphorylation
    Heather L Ball
    Department of Biochemistry, Vanderbilt University, Nashville, TN 37232, USA
    Mol Biol Cell 16:2372-81. 2005
    ..These data suggest that binding to RPA-ssDNA is not the essential function of ATRIP in ATR-dependent checkpoint signaling and ATR has an important function in properly localizing the ATR-ATRIP complex...
  9. pmc Cyclin-dependent kinase 2 dependent phosphorylation of ATRIP regulates the G2-M checkpoint response to DNA damage
    Jeremy S Myers
    Department of Biochemistry and Mass Spectrometry Research Center, Vanderbilt University, 23rd Pierce Avenue, Nashville, TN 37232, USA
    Cancer Res 67:6685-90. 2007
    ..Thus, ATRIP is a CDK2 substrate, and CDK2-dependent phosphorylation of S224 regulates the ability of ATR-ATRIP to promote cell cycle arrest in response to DNA damage...
  10. pmc DDB1 maintains genome integrity through regulation of Cdt1
    Courtney A Lovejoy
    Department of Biochemistry, Vanderbilt University, Nashville, TN 37232, USA
    Mol Cell Biol 26:7977-90. 2006
    ..Therefore, DDB1 prevents DNA lesions from accumulating in replicating human cells, in part by regulating Cdt1 degradation...
  11. pmc ATRIP oligomerization is required for ATR-dependent checkpoint signaling
    Heather L Ball
    Department of Biochemistry, Vanderbilt University, Nashville, Tennessee 37232, USA
    J Biol Chem 280:31390-6. 2005
    ..Thus, the ATR-ATRIP complex exists in higher order oligomeric states within cells and ATRIP oligomerization is essential for its function...
  12. pmc Function of a conserved checkpoint recruitment domain in ATRIP proteins
    Heather L Ball
    Department of Biochemistry, Vanderbilt University, Nashville, TN 37232, USA
    Mol Cell Biol 27:3367-77. 2007
    ..Our results support the idea of a multistep model for ATR activation that requires separable localization and activation functions of ATRIP...
  13. pmc The basic cleft of RPA70N binds multiple checkpoint proteins, including RAD9, to regulate ATR signaling
    Xin Xu
    Department of Biochemistry, Vanderbilt University School of Medicine, 613 Light Hall, 23rd at Pierce Avenue, Nashville, TN 37232, USA
    Mol Cell Biol 28:7345-53. 2008
    ..Thus, the basic cleft of the RPA70 N-terminal OB-fold domain binds multiple checkpoint proteins, including RAD9, to promote ATR signaling...
  14. pmc Functional genomic screens identify CINP as a genome maintenance protein
    Courtney A Lovejoy
    Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    Proc Natl Acad Sci U S A 106:19304-9. 2009
    ..CINP interacts with ATR-interacting protein and regulates ATR-dependent signaling, resistance to replication stress, and G2 checkpoint integrity...
  15. pmc Deletion of histone deacetylase 3 reveals critical roles in S phase progression and DNA damage control
    Srividya Bhaskara
    Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    Mol Cell 30:61-72. 2008
    ..Moreover, we noted that Hdac3-/- MEFs were protected from DNA damage when quiescent, which may provide a mechanistic basis for the action of HDAC inhibitors on cycling tumor cells...
  16. pmc TopBP1 activates ATR through ATRIP and a PIKK regulatory domain
    Daniel A Mordes
    Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    Genes Dev 22:1478-89. 2008
    ..Therefore, divergent amino acid sequences within the PRD and a unique protein partner allow each of these PIK kinases to respond to distinct cellular events...
  17. pmc Dpb11 activates the Mec1-Ddc2 complex
    Daniel A Mordes
    Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    Proc Natl Acad Sci U S A 105:18730-4. 2008
    ..Thus, Dpb11 is a functional ortholog of human TopBP1, and the Mec1/ATR activation mechanism is conserved from yeast to humans...
  18. pmc Activation of ATR and related PIKKs
    Daniel A Mordes
    Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    Cell Cycle 7:2809-12. 2008
    ..A further understanding of the mechanism of ATR activation will likely provide insights into the regulation of related PIKKs...
  19. pmc SOSS1/2: Sensors of single-stranded DNA at a break
    Edward A Nam
    Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    Mol Cell 35:258-9. 2009
    ..In this issue of Molecular Cell, Huang et al. (2009) describe two heterotrimeric single-stranded DNA binding complexes, SOSS1 and SOSS2, that function downstream of the MRN complex to promote DNA repair and the G2/M checkpoint...
  20. pmc The annealing helicase SMARCAL1 maintains genome integrity at stalled replication forks
    Carol E Bansbach
    Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    Genes Dev 23:2405-14. 2009
    ..Thus, SMARCAL1 is a replication stress response protein, and the pleiotropic phenotypes of SIOD are at least partly due to defects in genome maintenance during DNA replication...
  21. pmc Common mechanisms of PIKK regulation
    Courtney A Lovejoy
    Department of Biochemistry, Vanderbilt University School of Medicine, 613 Light Hall, Nashville, TN 37232, USA
    DNA Repair (Amst) 8:1004-8. 2009
    ..We outline common regulatory themes and suggest how discoveries about the regulation of one PIKK can be informative for the other family members...

Research Grants13

  1. Function of the ATR-ATRIP complex
    DAVID K contact CORTEZ; Fiscal Year: 2010
    ..Therefore, these mechanistic studies focus on highly significant biological questions. ..
  2. Function of the ATR-ATRIP complex
    David Cortez; Fiscal Year: 2009
    ..Thus, the mechanistic understanding of how DNA damage response pathways operate is a highly significant research objective. ..
  3. Functional Analysis of Replication Stress Response Proteins
    David Cortez; Fiscal Year: 2009
    ..The genome is constantly exposed to environmental and endogenous genotoxic insults that challenge DNA replication. This research proposal aims to define the genome maintenance functions of novel replication stress response proteins. ..
  4. Function of the ATR-ATRIP complex
    David Cortez; Fiscal Year: 2009
    ..Therefore, these mechanistic studies focus on highly significant biological questions. ..
  5. Function of the ATR-ATRIP complex
    David Cortez; Fiscal Year: 2007
    ..In addition, many cancer therapies activate these pathways. Therefore, a mechanistic understanding of checkpoint signaling pathways is essential to understand how cancer develops and how we may better diagnose and treat cancer patients. ..
  6. Signaling by the ATM and ATR Kinases
    David Cortez; Fiscal Year: 2006
    ..The award will facilitate the candidate's transition from a mentored research environment to an independent academic research position focused on basic cancer biology. ..
  7. Functional Analysis of Replication Stress Response Proteins
    DAVID K CORTEZ; Fiscal Year: 2010
    ..The genome is constantly exposed to environmental and endogenous genotoxic insults that challenge DNA replication. This research proposal aims to define the genome maintenance functions of novel replication stress response proteins. ..