Affiliation: Vanderbilt University
Ju B, Chen W, Orr B, Spitsbergen J, Jia S, Eden C, et al
. Oncogenic KRAS promotes malignant brain tumors in zebrafish. Mol Cancer. 2015;14:18 pubmed publisher
Maddison L, Joest K, Kammeyer R, Chen W. Skeletal muscle insulin resistance in zebrafish induces alterations in Î²-cell number and glucose tolerance in an age- and diet-dependent manner. Am J Physiol Endocrinol Metab. 2015;308:E662-9 pubmed publisher
Yin L, Maddison L, Li M, Kara N, LaFave M, Varshney G, et al
. Multiplex Conditional Mutagenesis Using Transgenic Expression of Cas9 and sgRNAs. Genetics. 2015;200:431-41 pubmed publisher
..Our findings suggest that CRISPR/Cas9-based conditional mutagenesis in zebrafish is not only feasible but rapid and straightforward. ..
Li M, Dean E, Zhao L, Nicholson W, Powers A, Chen W. Glucagon receptor inactivation leads to Î±-cell hyperplasia in zebrafish. J Endocrinol. 2015;227:93-103 pubmed publisher
..The robust Î±-cell hyperplasia in gcgra-/-;gcgrb-/- larvae provides a platform to screen for chemical and genetic suppressors, and ultimately to identify the stimulus of Î±-cell hyperplasia and its signaling mechanism. ..
Li M, Page McCaw P, Chen W. FGF1 Mediates Overnutrition-Induced Compensatory Î²-Cell Differentiation. Diabetes. 2016;65:96-109 pubmed publisher
..Thus, the recently discovered antidiabetes function of FGF1 may act partially through increasing Î²-cell differentiation. ..
Li M, Zhao L, Page McCaw P, Chen W. Zebrafish Genome Engineering Using the CRISPR-Cas9 System. Trends Genet. 2016;32:815-827 pubmed publisher
..This tool has been widely adopted in the zebrafish model; we discuss the development of this system in the zebrafish and how it can be manipulated to facilitate genome engineering. ..