Raymond Burk

Summary

Affiliation: Vanderbilt University
Country: USA

Publications

  1. ncbi request reprint Selenoprotein P and apolipoprotein E receptor-2 interact at the blood-brain barrier and also within the brain to maintain an essential selenium pool that protects against neurodegeneration
    Raymond F Burk
    Division of Gastroenterology, Hepatology, and Nutrition, Department of Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee, USA
    FASEB J 28:3579-88. 2014
  2. pmc Maternal-fetal transfer of selenium in the mouse
    Raymond F Burk
    Division of Gastroenterology, Hepatology, and Nutrition, Department of Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee, USA
    FASEB J 27:3249-56. 2013
  3. pmc Extracellular glutathione peroxidase (Gpx3) binds specifically to basement membranes of mouse renal cortex tubule cells
    Gary E Olson
    Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, Tennessee, USA
    Am J Physiol Renal Physiol 298:F1244-53. 2010
  4. ncbi request reprint Effects of chemical form of selenium on plasma biomarkers in a high-dose human supplementation trial
    Raymond F Burk
    Division of Gastroenterology, Hepatology, and Nutrition, Department of Medicine, Vanderbilt University School of Medicine, Nashville, TN 37232 0252, USA
    Cancer Epidemiol Biomarkers Prev 15:804-10. 2006
  5. ncbi request reprint A combined deficiency of vitamins E and C causes severe central nervous system damage in guinea pigs
    Raymond F Burk
    Division of Gastroenterology, Department of Medicine, Vanderbilt University, Nashville, Tennessee, USA
    J Nutr 136:1576-81. 2006
  6. pmc Deletion of selenoprotein P upregulates urinary selenium excretion and depresses whole-body selenium content
    Raymond F Burk
    Division of Gastroenterology, Hepatology, and Nutrition, Department of Medicine, Vanderbilt University School of Medicine, Nashville, TN 37232 0252, USA
    Biochim Biophys Acta 1760:1789-93. 2006
  7. ncbi request reprint Deletion of apolipoprotein E receptor-2 in mice lowers brain selenium and causes severe neurological dysfunction and death when a low-selenium diet is fed
    Raymond F Burk
    Division of Gastroenterology, Hepatology, and Nutrition, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    J Neurosci 27:6207-11. 2007
  8. pmc Selenium deficiency activates mouse liver Nrf2-ARE but vitamin E deficiency does not
    Raymond F Burk
    Division of Gastroenterology, Hepatology, and Nutrition, Department of Medicine, Vanderbilt University School of Medicine, Nashville, TN 37232 0252, USA
    Free Radic Biol Med 44:1617-23. 2008
  9. pmc Selenoprotein P-expression, functions, and roles in mammals
    Raymond F Burk
    Division of Gastroenterology, Hepatology, and Nutrition, Department of Medicine, 1030C Medical Research Building IV, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0252, USA
    Biochim Biophys Acta 1790:1441-7. 2009
  10. pmc Glutathione peroxidase-3 produced by the kidney binds to a population of basement membranes in the gastrointestinal tract and in other tissues
    Raymond F Burk
    Division of Gastroenterology, Hepatology, and Nutrition, Department of Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee, USA
    Am J Physiol Gastrointest Liver Physiol 301:G32-8. 2011

Collaborators

Detail Information

Publications37

  1. ncbi request reprint Selenoprotein P and apolipoprotein E receptor-2 interact at the blood-brain barrier and also within the brain to maintain an essential selenium pool that protects against neurodegeneration
    Raymond F Burk
    Division of Gastroenterology, Hepatology, and Nutrition, Department of Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee, USA
    FASEB J 28:3579-88. 2014
    ..P., Kurokawa, S., Mitchell, S. L., Zhang, W. Selenoprotein P and apolipoprotein E receptor-2 interact at the blood-brain barrier and also within the brain to maintain an essential selenium pool that protects against neurodegeneration. ..
  2. pmc Maternal-fetal transfer of selenium in the mouse
    Raymond F Burk
    Division of Gastroenterology, Hepatology, and Nutrition, Department of Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee, USA
    FASEB J 27:3249-56. 2013
    ..The failure of their disruptions (apoER2 deletion, Gpx3 deletion) to affect fetal selenium under selenium-adequate conditions indicates the existence of an additional maternal-fetal selenium transfer mechanism...
  3. pmc Extracellular glutathione peroxidase (Gpx3) binds specifically to basement membranes of mouse renal cortex tubule cells
    Gary E Olson
    Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, Tennessee, USA
    Am J Physiol Renal Physiol 298:F1244-53. 2010
    ..The specific binding of a large pool of Gpx3 to basement membranes in the kidney cortex strongly suggests a need for glutathione peroxidase activity in the cortical peritubular space...
  4. ncbi request reprint Effects of chemical form of selenium on plasma biomarkers in a high-dose human supplementation trial
    Raymond F Burk
    Division of Gastroenterology, Hepatology, and Nutrition, Department of Medicine, Vanderbilt University School of Medicine, Nashville, TN 37232 0252, USA
    Cancer Epidemiol Biomarkers Prev 15:804-10. 2006
    ..Plasma selenium seems to reflect the selenomethionine content of yeast but not the other yeast selenium forms. As judged by urinary selenium excretion, selenium in the form of selenomethionine is better absorbed than selenite...
  5. ncbi request reprint A combined deficiency of vitamins E and C causes severe central nervous system damage in guinea pigs
    Raymond F Burk
    Division of Gastroenterology, Department of Medicine, Vanderbilt University, Nashville, Tennessee, USA
    J Nutr 136:1576-81. 2006
    ..These results indicate that the paralysis and death caused by combined deficiency of vitamins E and C in guinea pigs is caused by severe damage in the brainstem and spinal cord...
  6. pmc Deletion of selenoprotein P upregulates urinary selenium excretion and depresses whole-body selenium content
    Raymond F Burk
    Division of Gastroenterology, Hepatology, and Nutrition, Department of Medicine, Vanderbilt University School of Medicine, Nashville, TN 37232 0252, USA
    Biochim Biophys Acta 1760:1789-93. 2006
    ....
  7. ncbi request reprint Deletion of apolipoprotein E receptor-2 in mice lowers brain selenium and causes severe neurological dysfunction and death when a low-selenium diet is fed
    Raymond F Burk
    Division of Gastroenterology, Hepatology, and Nutrition, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    J Neurosci 27:6207-11. 2007
    ....
  8. pmc Selenium deficiency activates mouse liver Nrf2-ARE but vitamin E deficiency does not
    Raymond F Burk
    Division of Gastroenterology, Hepatology, and Nutrition, Department of Medicine, Vanderbilt University School of Medicine, Nashville, TN 37232 0252, USA
    Free Radic Biol Med 44:1617-23. 2008
    ..We conclude that Se deficiency causes cytosolic oxidative stress but that vitamin E deficiency does not. This suggests that the oxidant defense mechanisms in which these antioxidant nutrients function are independent of one another...
  9. pmc Selenoprotein P-expression, functions, and roles in mammals
    Raymond F Burk
    Division of Gastroenterology, Hepatology, and Nutrition, Department of Medicine, 1030C Medical Research Building IV, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0252, USA
    Biochim Biophys Acta 1790:1441-7. 2009
    ..Thus, Sepp1 has several functions. In addition, plasma Sepp1 concentration falls in selenium deficiency and, therefore, it can be used as an index of selenium nutritional status...
  10. pmc Glutathione peroxidase-3 produced by the kidney binds to a population of basement membranes in the gastrointestinal tract and in other tissues
    Raymond F Burk
    Division of Gastroenterology, Hepatology, and Nutrition, Department of Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee, USA
    Am J Physiol Gastrointest Liver Physiol 301:G32-8. 2011
    ..In the other compartment, the epididymis provides Gpx3 to its own lumen. Tissues other than kidney and epididymis express Gpx3 at lower levels and may supply Gpx3 to other compartments...
  11. ncbi request reprint Selenoprotein P: an extracellular protein with unique physical characteristics and a role in selenium homeostasis
    Raymond F Burk
    Division of Gastroenterology, Department of Medicine and Center in Molecular Toxicology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    Annu Rev Nutr 25:215-35. 2005
    ....
  12. ncbi request reprint Selenoprotein metabolism and function: evidence for more than one function for selenoprotein P
    Raymond F Burk
    Division of Gastroenterology, Department of Medicine and Clinical Nutrition Research Unit, Vanderbilt University School of Medicine, Nashville, TN 37232 2279, USA
    J Nutr 133:1517S-20S. 2003
    ..Thus, there is evidence for both antioxidant and selenium transport functions of selenoprotein P...
  13. ncbi request reprint Deletion of selenoprotein P alters distribution of selenium in the mouse
    Kristina E Hill
    Division of Gastroenterology, Department of Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    J Biol Chem 278:13640-6. 2003
    ..Further, they indicate that transport forms of selenium other than Se-P exist because selenium levels of all tissues except testis responded to increases of dietary selenium in Sepp(-/-) mice...
  14. ncbi request reprint Neurological dysfunction occurs in mice with targeted deletion of the selenoprotein P gene
    Kristina E Hill
    Division of Gastroenterology and Clinical Nutrition Research Unit, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    J Nutr 134:157-61. 2004
    ..Both the absence of selenoprotein P and a low dietary selenium supply are necessary for the dysfunction to occur, indicating that selenoprotein P and at least one other form of selenium supply the element to the brain...
  15. doi request reprint Neurodegeneration in mice resulting from loss of functional selenoprotein P or its receptor apolipoprotein E receptor 2
    William M Valentine
    Department of Pathology, Division of Gastroenterology, Hepatology and Nutrition, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0252, USA
    J Neuropathol Exp Neurol 67:68-77. 2008
    ..Those areas have high metabolic rates. We conclude that interference with Sepp1 function damages auditory and motor areas, at least in part by restricting selenium supply to the brain regions...
  16. ncbi request reprint Mass spectrometric identification of N- and O-glycosylation sites of full-length rat selenoprotein P and determination of selenide-sulfide and disulfide linkages in the shortest isoform
    Shuguang Ma
    Mass Spectrometry Research Center, Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    Biochemistry 42:9703-11. 2003
    ..The disulfide formed by Cys153-Cys156 also has some characteristics of a redox active pair...
  17. ncbi request reprint All regions of mouse brain are dependent on selenoprotein P for maintenance of selenium
    Akihiro Nakayama
    Division of Gastroenterology, Hepatology, and Nutrition, Department of Medicine, Vanderbilt University School of Medicine, Nashville, TN 37232 0252, USA
    J Nutr 137:690-3. 2007
    ..These results suggest that Sepp1 is more important for maintaining selenium in the hippocampus than in other brain regions. They also confirm the position of the brain at the apex of the organ selenium hierarchy...
  18. ncbi request reprint Loss of activity of the selenoenzyme thioredoxin reductase causes induction of hepatic heme oxygenase-1
    Volker Mostert
    Division of Gastroenterology, Vanderbilt University School of Medicine, Nashville, TN 37232 2279, USA
    FEBS Lett 541:85-8. 2003
    ..In the rat hepatoma cell line H4IIE, inhibition of TrxR by aurothioglucose or by 1-chloro-2,4-dinitrobenzene led to induction of HO-1. We conclude that loss of TrxR is responsible for the induction of HO-1 by selenium deficiency...
  19. ncbi request reprint Apolipoprotein E receptor-2 (ApoER2) mediates selenium uptake from selenoprotein P by the mouse testis
    Gary E Olson
    Department of Cell and Developmental Biology, Department of Medicine, Vanderbilt University, Nashville, Tennessee 37232, USA
    J Biol Chem 282:12290-7. 2007
    ..Co-immunoprecipitation analysis demonstrated an interaction of testis ApoER2 with Sepp1. These data demonstrate that Sertoli cell ApoER2 is a Sepp1 receptor and a component of the selenium delivery pathway to spermatogenic cells...
  20. ncbi request reprint The selenium-rich C-terminal domain of mouse selenoprotein P is necessary for the supply of selenium to brain and testis but not for the maintenance of whole body selenium
    Kristina E Hill
    Division of Gastroenterology, Hepatology, and Nutrition, Department of Medicine, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    J Biol Chem 282:10972-80. 2007
    ..The results of this study demonstrate that the C terminus of Sepp1 is critical for the maintenance of selenium in brain and testis but not for the maintenance of whole body selenium...
  21. pmc Combined selenium and vitamin C deficiency causes cell death in guinea pig skeletal muscle
    Kristina E Hill
    Division of Gastroenterology, Hepatology, and Nutrition, Department of Medicine, Vanderbilt University School of Medicine, Nashville, TN 37232 0252, USA
    Nutr Res 29:213-9. 2009
    ..The elevation of muscle F2 isoprostanes is compatible with the cell death being caused by oxidative stress...
  22. ncbi request reprint Sequential development of flagellar defects in spermatids and epididymal spermatozoa of selenium-deficient rats
    Gary E Olson
    Department of Cell and Developmental Biology, Department of Medicine, Vanderbilt University, Nashville, Tennessee 37232, USA
    Reproduction 127:335-42. 2004
    ..These data suggest that the known loss of male fertility in selenium deficiency results from the sequential development of sperm defects expressed during both spermiogenesis and maturation in the epididymis...
  23. ncbi request reprint Combined deficiency of vitamins E and C causes paralysis and death in guinea pigs
    Kristina E Hill
    Division of Gastroenterology, Department of Medicine, Vanderbilt University School of Medicine, Nashville, TN, USA
    Am J Clin Nutr 77:1484-8. 2003
    ..On the basis of in vitro studies, the antioxidant nutrients vitamins E and C are postulated to interact in vivo...
  24. ncbi request reprint Brainstem axonal degeneration in mice with deletion of selenoprotein p
    William M Valentine
    Department of Pathology, Vanderbilt University School of Medicine, Vanderbilt University Medical Center, Nashville, Tennessee 37232 2561, USA
    Toxicol Pathol 33:570-6. 2005
    ....
  25. ncbi request reprint Selenium, an antioxidant nutrient
    Raymond F Burk
    Division of Gastroenterology, Department of Medicine, Clinical Nutrition Research Unit, Vanderbilt University Medical Center, Nashville, Tennessee 37232 2279, USA
    Nutr Clin Care 5:75-9. 2002
    ..Since most estimates of selenium intake in the United States are 80 micrograms per day or greater, routine selenium supplementation is not recommended in the United States...
  26. ncbi request reprint Selenoprotein P is required for mouse sperm development
    Gary E Olson
    Department of Cell and Developmental Biology, Vanderbilt University, Nashville, Tennessee 37232, USA
    Biol Reprod 73:201-11. 2005
    ..These data demonstrate that SEPP1 is required for development of functional spermatozoa and indicate that it is an essential component of the selenium delivery pathway for developing germ cells...
  27. ncbi request reprint Thioredoxin reductase reduces lipid hydroperoxides and spares alpha-tocopherol
    James M May
    Department of Medicine, Vanderbilt University School of Medicine, 715 Preston Research Building II, 2220 Pierce Avenue, Nashville, TN 37232 6303, USA
    Biochem Biophys Res Commun 292:45-9. 2002
    ..Thioredoxin reductase also decreased F(2)-isoprostanes in ghosts oxidized by ferricyanide, suggesting that its ability to spare alpha-tocopherol relates to reduction of lipid hydroperoxides...
  28. doi request reprint Megalin mediates selenoprotein P uptake by kidney proximal tubule epithelial cells
    Gary E Olson
    Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    J Biol Chem 283:6854-60. 2008
    ..These results demonstrate that kidney selenium homeostasis is mediated by a megalin-dependent Sepp1 uptake pathway in the proximal tubule...
  29. ncbi request reprint Serum iron increases with acute induction of hepatic heme oxygenase-1 in mice
    Volker Mostert
    Department of Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee, USA
    Drug Metab Rev 39:619-26. 2007
    ..Further, they link HO-1 induction with a rise in serum iron, suggesting that the iron resulting from catabolism of heme by HO-1 is released by the liver...
  30. ncbi request reprint Selective induction of liver parenchymal cell heme oxygenase-1 in selenium-deficient rats
    Volker Mostert
    Division of Gastroenterology, Department of Medicine and Clinical Nutrition Research Unit, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    Biol Chem 384:681-7. 2003
    ..We speculate that selenium deficiency affects hepatic antioxidant selenoproteins, resulting in an up-regulation of HO-1...
  31. ncbi request reprint Loss of selenium from selenoproteins: conversion of selenocysteine to dehydroalanine in vitro
    Shuguang Ma
    Mass Spectrometry Research Center, Vanderbilt University, Nashville, Tennessee 37232 6400, USA
    J Am Soc Mass Spectrom 14:593-600. 2003
    ..The loss of selenium from selenoproteins is probably through the mechanism of oxidation of selenocysteine residue to selenoxide followed by syn-beta-elimination of selenenic acid during sample processing...
  32. ncbi request reprint Mass spectrometric determination of selenenylsulfide linkages in rat selenoprotein P
    Shuguang Ma
    Mass Spectrometry Research Center, Vanderbilt University, Nashville, Tennessee 37232, USA
    J Mass Spectrom 40:400-4. 2005
    ..It is concluded that selenoprotein P contains these two selenenylsulfide bonds...
  33. ncbi request reprint Mass spectrometric characterization of full-length rat selenoprotein P and three isoforms shortened at the C terminus. Evidence that three UGA codons in the mRNA open reading frame have alternative functions of specifying selenocysteine insertion or trans
    Shuguang Ma
    Mass Spectrometry Research Center, Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232 2279, USA
    J Biol Chem 277:12749-54. 2002
    ....
  34. pmc Plasma selenium measurements in subjects from areas with contrasting gastric cancer risks in Colombia
    M Constanza Camargo
    Department of Medicine, School of Medicine, Division of Gastroenterology, Hepatology and Nutrition, Vanderbilt University, Nashville, Tennessee 37232 0253, USA
    Arch Med Res 39:443-51. 2008
    ..This pilot study aimed to determine and compare selenium status in two Colombian populations with different gastric cancer risks: a high-risk area in the volcanic region of the Andes Mountains and a low-risk area on the Pacific coast...
  35. ncbi request reprint Alternatively activated myeloid cells limit pathogenicity associated with African trypanosomiasis through the IL-10 inducible gene selenoprotein P
    Tom Bosschaerts
    Department of Molecular and Cellular Interactions, Laboratory of Cellular and Molecular Immunology, Vrije Universiteit Brussel, Pleinlaan 2, Brussels, Belgium
    J Immunol 180:6168-75. 2008
    ..These data validate M2-associated molecules as functioning in reducing the impact of parasite infection on the host...
  36. ncbi request reprint Serum selenium measurements in women with early-stage breast cancer with and without chemotherapy-induced ovarian failure
    Hollie A Breedlove
    Department of Human Nutrition, The Ohio State University, Columbus, OH 43210, USA
    Breast Cancer Res Treat 97:225-30. 2006
    ..Selenium status in premenopausal breast cancer patients, as measured by serum selenium, GPx and SelP, was within the normal range before and following adjuvant chemotherapy, and was not affected by chemotherapy-induced ovarian failure...
  37. ncbi request reprint Effectiveness of selenium supplements in a low-selenium area of China
    Yiming Xia
    Institute of Nutrition and Food Hygiene, Chinese Academy of Preventive Medicine, Beijing, China
    Am J Clin Nutr 81:829-34. 2005
    ..Although there is no evidence of selenium deficiency in the United States, people in many other areas of the world are selenium deficient, with the consequence that they are unable to express their selenoproteins fully...

Research Grants34

  1. HUMAN SELENIUM NUTRITIONAL REQUIREMENT AND BIOMARKERS
    Raymond Burk; Fiscal Year: 2002
    ..They should also provide tools for investigators who need to evaluate subjects for selenium deficiency and for intake of pharmacologic amounts of selenium. ..
  2. NUTRITIONAL AND METABOLIC SIGNIFICANCE OF SELENIUM
    Raymond Burk; Fiscal Year: 2002
    ..These and other studies are proposed to evaluate the role of Se-P in protecting endothelial cells (and other cells) against oxidant substances in the extracellular space. ..
  3. HUMAN SELENIUM NUTRITIONAL REQUIREMENT AND BIOMARKERS
    Raymond Burk; Fiscal Year: 2003
    ..They should also provide tools for investigators who need to evaluate subjects for selenium deficiency and for intake of pharmacologic amounts of selenium. ..
  4. NUTRITIONAL AND METABOLIC SIGNIFICANCE OF SELENIUM
    Raymond Burk; Fiscal Year: 2003
    ..These and other studies are proposed to evaluate the role of Se-P in protecting endothelial cells (and other cells) against oxidant substances in the extracellular space. ..
  5. HUMAN SELENIUM NUTRITIONAL REQUIREMENT AND BIOMARKERS
    Raymond Burk; Fiscal Year: 2004
    ..They should also provide tools for investigators who need to evaluate subjects for selenium deficiency and for intake of pharmacologic amounts of selenium. ..
  6. HUMAN SELENIUM NUTRITIONAL REQUIREMENT AND BIOMARKERS
    Raymond Burk; Fiscal Year: 2005
    ..They should also provide tools for investigators who need to evaluate subjects for selenium deficiency and for intake of pharmacologic amounts of selenium. ..
  7. Human Se Nutritional Requirement and Biomarkers in Health and Disease
    Raymond Burk; Fiscal Year: 2006
    ..All these studies are aimed at facilitating efforts to determine the health effects of selenium deficiency and to guide supplementation of the element when that is needed. ..
  8. Human Se Nutritional Requirement and Biomarkers in Health and Disease
    Raymond Burk; Fiscal Year: 2007
    ..All these studies are aimed at facilitating efforts to determine the health effects of selenium deficiency and to guide supplementation of the element when that is needed. ..
  9. Human Se Nutritional Requirement and Biomarkers in Health and Disease
    Raymond Burk; Fiscal Year: 2006
    ..All these studies are aimed at facilitating efforts to determine the health effects of selenium deficiency and to guide supplementation of the element when that is needed. ..
  10. NUTRITIONAL AND METABOLIC SIGNIFICANCE OF SELENIUM
    Raymond Burk; Fiscal Year: 2007
    ..Results of these studies will be interpreted to characterize the brain injury with the objective of selecting human neurological disorders to study for genetic abnormalities of Se-P and its receptor. ..
  11. Human Se Nutritional Requirement and Biomarkers in Health and Disease
    Raymond Burk; Fiscal Year: 2009
    ..All these studies are aimed at facilitating efforts to determine the health effects of selenium deficiency and to guide supplementation of the element when that is needed. ..
  12. Gpx3 Targeting and Extracellular Oxidative Stress
    Raymond Burk; Fiscal Year: 2009
    ..We produced mice with Gpx3 deleted and will assess Gpx3 protection against oxidative stress in those mice to shed light on some diseases. ..
  13. Human Se Nutritional Requirement and Biomarkers in Health and Disease
    Raymond F Burk; Fiscal Year: 2010
    ..All these studies are aimed at facilitating efforts to determine the health effects of selenium deficiency and to guide supplementation of the element when that is needed. ..
  14. NUTRITIONAL AND METABOLIC SIGNIFICANCE OF SELENIUM
    Raymond Burk; Fiscal Year: 2001
    ..These and other studies are proposed to evaluate the role of Se-P in protecting endothelial cells (and other cells) against oxidant substances in the extracellular space. ..
  15. HUMAN SELENIUM NUTRITIONAL REQUIREMENT AND BIOMARKERS
    Raymond Burk; Fiscal Year: 2001
    ..They should also provide tools for investigators who need to evaluate subjects for selenium deficiency and for intake of pharmacologic amounts of selenium. ..
  16. NUTRITIONAL AND METABOLIC SIGNIFICANCE OF SELENIUM
    Raymond Burk; Fiscal Year: 1999
    ..These and other studies are proposed to evaluate the role of Se-P in protecting endothelial cells (and other cells) against oxidant substances in the extracellular space. ..
  17. NUTRITIONAL AND METABOLIC SIGNIFICANCE OF SELENIUM
    Raymond Burk; Fiscal Year: 1990
    ..Finally, human selenoprotein P will be purified and an assay for it will be devised. These studies will extend our understanding of the function of selenium and could lead to a better understanding of oxidant defenses...
  18. NUTRITIONAL & METABOLIC SIGNIFICANCE OF SELENIUM
    Raymond Burk; Fiscal Year: 1991
    ..Finally, human selenoprotein P will be purified and an assay for it will be devised. These studies will extend our understanding of the function of selenium and could lead to a better understanding of oxidant defenses...
  19. NUTRITIONAL & METABOLIC SIGNIFICANCE OF SELENIUM
    Raymond Burk; Fiscal Year: 1992
    ..Finally, human selenoprotein P will be purified and an assay for it will be devised. These studies will extend our understanding of the function of selenium and could lead to a better understanding of oxidant defenses...
  20. NUTRITIONAL AND METABOLIC SIGNIFICANCE OF SELENIUM
    Raymond Burk; Fiscal Year: 1993
    ..Finally, human selenoprotein P will be purified and an assay for it will be devised. These studies will extend our understanding of the function of selenium and could lead to a better understanding of oxidant defenses...
  21. NUTRITIONAL AND METABOLIC SIGNIFICANCE OF SELENIUM
    Raymond Burk; Fiscal Year: 2000
    ..These and other studies are proposed to evaluate the role of Se-P in protecting endothelial cells (and other cells) against oxidant substances in the extracellular space. ..
  22. Gpx3 Targeting and Extracellular Oxidative Stress
    Raymond F Burk; Fiscal Year: 2010
    ..We produced mice with Gpx3 deleted and will assess Gpx3 protection against oxidative stress in those mice to shed light on some diseases. ..