R F Burk

Summary

Affiliation: Vanderbilt University
Country: USA

Publications

  1. ncbi request reprint Orphan selenoproteins
    R F Burk
    Department of Medicine and Clinical Nutrition Research Unit, Vanderbilt University School of Medicine, Nashville, TN 37232 2279, USA
    Bioessays 21:231-7. 1999
  2. ncbi request reprint Plasma selenium in specific and non-specific forms
    R F Burk
    Division of Gastroenterology, Department of Medicine and Clinical Nutrition Research Unit, Vanderbilt University School of Medicine, Nashville, TN 37221, USA
    Biofactors 14:107-14. 2001
  3. ncbi request reprint Selenium, an antioxidant nutrient
    Raymond F Burk
    Division of Gastroenterology, Department of Medicine, Clinical Nutrition Research Unit, Vanderbilt University Medical Center, Nashville, Tennessee 37232 2279, USA
    Nutr Clin Care 5:75-9. 2002
  4. ncbi request reprint Selenoprotein P associates with endothelial cells in rat tissues
    R F Burk
    Department of Medicine and Institute for Developmental Neuroscience, Vanderbilt University, Nashville, TN 37232 2279, USA
    Histochem Cell Biol 108:11-5. 1997
  5. ncbi request reprint Thioredoxin reductase activity is decreased by selenium deficiency
    K E Hill
    Department of Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    Biochem Biophys Res Commun 234:293-5. 1997
  6. ncbi request reprint Reduction of dehydroascorbate to ascorbate by the selenoenzyme thioredoxin reductase
    J M May
    Department of Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 6303, USA
    J Biol Chem 272:22607-10. 1997
  7. ncbi request reprint Effect of selenium on selenoprotein P expression in cultured liver cells
    K E Hill
    Department of Medicine GI and Clinical Nutrition Research Unit, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    Biochim Biophys Acta 1313:29-34. 1996
  8. pmc Conserved nucleotide sequences in the open reading frame and 3' untranslated region of selenoprotein P mRNA
    K E Hill
    Department of Medicine, Vanderbilt University School of Medicine, Nashville, TN 37232
    Proc Natl Acad Sci U S A 90:537-41. 1993
  9. ncbi request reprint Reduction of the ascorbyl free radical to ascorbate by thioredoxin reductase
    J M May
    Department of Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 6303, USA
    J Biol Chem 273:23039-45. 1998
  10. ncbi request reprint Comparison of formation of D2/E2-isoprostanes and F2-isoprostanes in vitro and in vivo--effects of oxygen tension and glutathione
    J D Morrow
    Department of Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 6602, USA
    Arch Biochem Biophys 353:160-71. 1998

Collaborators

Detail Information

Publications49

  1. ncbi request reprint Orphan selenoproteins
    R F Burk
    Department of Medicine and Clinical Nutrition Research Unit, Vanderbilt University School of Medicine, Nashville, TN 37232 2279, USA
    Bioessays 21:231-7. 1999
    ..It can be predicted that more proteins will be discovered that take advantage of the chemical properties of selenium...
  2. ncbi request reprint Plasma selenium in specific and non-specific forms
    R F Burk
    Division of Gastroenterology, Department of Medicine and Clinical Nutrition Research Unit, Vanderbilt University School of Medicine, Nashville, TN 37221, USA
    Biofactors 14:107-14. 2001
    ..These forms of the element appear to be metabolized by specific selenium metabolic processes...
  3. ncbi request reprint Selenium, an antioxidant nutrient
    Raymond F Burk
    Division of Gastroenterology, Department of Medicine, Clinical Nutrition Research Unit, Vanderbilt University Medical Center, Nashville, Tennessee 37232 2279, USA
    Nutr Clin Care 5:75-9. 2002
    ..Since most estimates of selenium intake in the United States are 80 micrograms per day or greater, routine selenium supplementation is not recommended in the United States...
  4. ncbi request reprint Selenoprotein P associates with endothelial cells in rat tissues
    R F Burk
    Department of Medicine and Institute for Developmental Neuroscience, Vanderbilt University, Nashville, TN 37232 2279, USA
    Histochem Cell Biol 108:11-5. 1997
    ..The heparin-binding properties of selenoprotein P could be the basis for its binding to tissue. Its localization to the vicinity of endothelial cells is potentially relevant to its oxidant defense function...
  5. ncbi request reprint Thioredoxin reductase activity is decreased by selenium deficiency
    K E Hill
    Department of Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    Biochem Biophys Res Commun 234:293-5. 1997
    ..These results indicate that thioredoxin reductase activity in liver and kidney is sensitive to selenium nutritional status but that brain thioredoxin reductase activity is less sensitive...
  6. ncbi request reprint Reduction of dehydroascorbate to ascorbate by the selenoenzyme thioredoxin reductase
    J M May
    Department of Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 6303, USA
    J Biol Chem 272:22607-10. 1997
    ..These results show that the thioredoxin system can reduce dehydroascorbate, and that this function is required for maintenance of liver ascorbate content...
  7. ncbi request reprint Effect of selenium on selenoprotein P expression in cultured liver cells
    K E Hill
    Department of Medicine GI and Clinical Nutrition Research Unit, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    Biochim Biophys Acta 1313:29-34. 1996
    ..Selenoprotein P secretion was inhibited by dithiothreitol, suggesting that disulfide bond formation is necessary for secretion of the mature protein...
  8. pmc Conserved nucleotide sequences in the open reading frame and 3' untranslated region of selenoprotein P mRNA
    K E Hill
    Department of Medicine, Vanderbilt University School of Medicine, Nashville, TN 37232
    Proc Natl Acad Sci U S A 90:537-41. 1993
    ..Because only two stem-loop structures are present in the 3' untranslated region of selenoprotein P mRNA, it can be concluded that a separate stem-loop structure is not required for each selenocysteine residue...
  9. ncbi request reprint Reduction of the ascorbyl free radical to ascorbate by thioredoxin reductase
    J M May
    Department of Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 6303, USA
    J Biol Chem 273:23039-45. 1998
    ..These results suggest that thioredoxin reductase can function as a cytosolic ascorbyl free radical reductase that may complement cellular ascorbate recycling by membrane-bound NADH-dependent reductases...
  10. ncbi request reprint Comparison of formation of D2/E2-isoprostanes and F2-isoprostanes in vitro and in vivo--effects of oxygen tension and glutathione
    J D Morrow
    Department of Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 6602, USA
    Arch Biochem Biophys 353:160-71. 1998
    ..These studies indicate that GSH promotes F2-IsoP formation and diminishes D2/E2-IsoP levels in vitro and in vivo by causing reduction of IsoP endoperoxides...
  11. ncbi request reprint Mitochondrial uptake and recycling of ascorbic acid
    X Li
    Department of Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee 37232-6303, USA
    Arch Biochem Biophys 387:143-53. 2001
    ..These results show that mitochondria can recycle ascorbate, which in turn might help to prevent deleterious effects of oxidant stress in the organelle...
  12. ncbi request reprint Combined selenium and vitamin E deficiency causes fatal myopathy in guinea pigs
    K E Hill
    Divisions of Gastroenterology and Endocrinology, Department of Medicine and Clinical Nutrition Research Unit, Vanderbilt University School of Medicine, Nashville, TN 37232 2279, USA
    J Nutr 131:1798-802. 2001
    ..This nutritional myopathy is much more severe than the myopathy that occurs with vitamin E deficiency alone...
  13. ncbi request reprint Centrilobular endothelial cell injury by diquat in the selenium-deficient rat liver
    J B Atkinson
    Department of Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee, USA
    Lab Invest 81:193-200. 2001
    ..We postulate that selenoprotein P protects the centrilobular endothelial cells against injury by oxidant molecules that result from diquat administration...
  14. ncbi request reprint Selenoprotein P. A selenium-rich extracellular glycoprotein
    R F Burk
    Department of Medicine, Vanderbilt University School of Medicine, Nashville, TN 37232
    J Nutr 124:1891-7. 1994
    ..More research will be required to test this hypothesis and to establish the biochemical function of selenoprotein P...
  15. ncbi request reprint Heparin-binding histidine and lysine residues of rat selenoprotein P
    R J Hondal
    Division of Gastroenterology, Department of Medicine, Clinical Nutrition Research Unit, Mass Spectrometry Research Center, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    J Biol Chem 276:15823-31. 2001
    ..0, explains the release of selenoprotein P from heparin binding as pH rises above 7.0. It can be speculated that this property would lead to increased binding of selenoprotein P in tissue regions that have low pH...
  16. ncbi request reprint Selenium spares ascorbate and alpha-tocopherol in cultured liver cell lines under oxidant stress
    X Li
    Department of Medicine, 715 Medical Research Building II, Vanderbilt University School of Medicine, Nashville, TN 37232-6303, USA
    FEBS Lett 508:489-92. 2001
    ..Whereas TR contributes little to ascorbate recycling in H4IIE cells, selenium spares ascorbate in culture and alpha-tocopherol in response to an oxidant stress...
  17. ncbi request reprint Purification of selenoprotein P from human plasma
    B Akesson
    Department of Medicine, Vanderbilt University, Nashville, TN 37232 2279
    Biochim Biophys Acta 1204:243-9. 1994
    ..one-third of the selenium from plasma or 0.4 mumol Se/l at a total selenium concentration of 1.1 mumol/l, indicating that selenoprotein P constituted this proportion of total plasma selenium in healthy US blood donors...
  18. ncbi request reprint Human selenoprotein P gene maps to 5q31
    K E Hill
    Department of Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    Genomics 36:550-1. 1996
  19. ncbi request reprint The cDNA for rat selenoprotein P contains 10 TGA codons in the open reading frame
    K E Hill
    Department of Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee 37232
    J Biol Chem 266:10050-3. 1991
    ..The primary structure of selenoprotein P suggests that it might be responsible for some of the antioxidant properties of selenium...
  20. ncbi request reprint Effects of chemical form of selenium on plasma biomarkers in a high-dose human supplementation trial
    Raymond F Burk
    Division of Gastroenterology, Hepatology, and Nutrition, Department of Medicine, Vanderbilt University School of Medicine, Nashville, TN 37232 0252, USA
    Cancer Epidemiol Biomarkers Prev 15:804-10. 2006
    ..Plasma selenium seems to reflect the selenomethionine content of yeast but not the other yeast selenium forms. As judged by urinary selenium excretion, selenium in the form of selenomethionine is better absorbed than selenite...
  21. ncbi request reprint A combined deficiency of vitamins E and C causes severe central nervous system damage in guinea pigs
    Raymond F Burk
    Division of Gastroenterology, Department of Medicine, Vanderbilt University, Nashville, Tennessee, USA
    J Nutr 136:1576-81. 2006
    ..These results indicate that the paralysis and death caused by combined deficiency of vitamins E and C in guinea pigs is caused by severe damage in the brainstem and spinal cord...
  22. ncbi request reprint Serum selenium measurements in women with early-stage breast cancer with and without chemotherapy-induced ovarian failure
    Hollie A Breedlove
    Department of Human Nutrition, The Ohio State University, Columbus, OH 43210, USA
    Breast Cancer Res Treat 97:225-30. 2006
    ..Selenium status in premenopausal breast cancer patients, as measured by serum selenium, GPx and SelP, was within the normal range before and following adjuvant chemotherapy, and was not affected by chemotherapy-induced ovarian failure...
  23. pmc Deletion of selenoprotein P upregulates urinary selenium excretion and depresses whole-body selenium content
    Raymond F Burk
    Division of Gastroenterology, Hepatology, and Nutrition, Department of Medicine, Vanderbilt University School of Medicine, Nashville, TN 37232 0252, USA
    Biochim Biophys Acta 1760:1789-93. 2006
    ....
  24. ncbi request reprint The selenium-rich C-terminal domain of mouse selenoprotein P is necessary for the supply of selenium to brain and testis but not for the maintenance of whole body selenium
    Kristina E Hill
    Division of Gastroenterology, Hepatology, and Nutrition, Department of Medicine, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    J Biol Chem 282:10972-80. 2007
    ..The results of this study demonstrate that the C terminus of Sepp1 is critical for the maintenance of selenium in brain and testis but not for the maintenance of whole body selenium...
  25. ncbi request reprint All regions of mouse brain are dependent on selenoprotein P for maintenance of selenium
    Akihiro Nakayama
    Division of Gastroenterology, Hepatology, and Nutrition, Department of Medicine, Vanderbilt University School of Medicine, Nashville, TN 37232 0252, USA
    J Nutr 137:690-3. 2007
    ..These results suggest that Sepp1 is more important for maintaining selenium in the hippocampus than in other brain regions. They also confirm the position of the brain at the apex of the organ selenium hierarchy...
  26. ncbi request reprint Apolipoprotein E receptor-2 (ApoER2) mediates selenium uptake from selenoprotein P by the mouse testis
    Gary E Olson
    Department of Cell and Developmental Biology, Department of Medicine, Vanderbilt University, Nashville, Tennessee 37232, USA
    J Biol Chem 282:12290-7. 2007
    ..Co-immunoprecipitation analysis demonstrated an interaction of testis ApoER2 with Sepp1. These data demonstrate that Sertoli cell ApoER2 is a Sepp1 receptor and a component of the selenium delivery pathway to spermatogenic cells...
  27. ncbi request reprint Deletion of apolipoprotein E receptor-2 in mice lowers brain selenium and causes severe neurological dysfunction and death when a low-selenium diet is fed
    Raymond F Burk
    Division of Gastroenterology, Hepatology, and Nutrition, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    J Neurosci 27:6207-11. 2007
    ....
  28. ncbi request reprint Serum iron increases with acute induction of hepatic heme oxygenase-1 in mice
    Volker Mostert
    Department of Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee, USA
    Drug Metab Rev 39:619-26. 2007
    ..Further, they link HO-1 induction with a rise in serum iron, suggesting that the iron resulting from catabolism of heme by HO-1 is released by the liver...
  29. doi request reprint Neurodegeneration in mice resulting from loss of functional selenoprotein P or its receptor apolipoprotein E receptor 2
    William M Valentine
    Department of Pathology, Division of Gastroenterology, Hepatology and Nutrition, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0252, USA
    J Neuropathol Exp Neurol 67:68-77. 2008
    ..Those areas have high metabolic rates. We conclude that interference with Sepp1 function damages auditory and motor areas, at least in part by restricting selenium supply to the brain regions...
  30. doi request reprint Megalin mediates selenoprotein P uptake by kidney proximal tubule epithelial cells
    Gary E Olson
    Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    J Biol Chem 283:6854-60. 2008
    ..These results demonstrate that kidney selenium homeostasis is mediated by a megalin-dependent Sepp1 uptake pathway in the proximal tubule...
  31. pmc Selenium deficiency activates mouse liver Nrf2-ARE but vitamin E deficiency does not
    Raymond F Burk
    Division of Gastroenterology, Hepatology, and Nutrition, Department of Medicine, Vanderbilt University School of Medicine, Nashville, TN 37232 0252, USA
    Free Radic Biol Med 44:1617-23. 2008
    ..We conclude that Se deficiency causes cytosolic oxidative stress but that vitamin E deficiency does not. This suggests that the oxidant defense mechanisms in which these antioxidant nutrients function are independent of one another...
  32. ncbi request reprint Alternatively activated myeloid cells limit pathogenicity associated with African trypanosomiasis through the IL-10 inducible gene selenoprotein P
    Tom Bosschaerts
    Department of Molecular and Cellular Interactions, Laboratory of Cellular and Molecular Immunology, Vrije Universiteit Brussel, Pleinlaan 2, Brussels, Belgium
    J Immunol 180:6168-75. 2008
    ..These data validate M2-associated molecules as functioning in reducing the impact of parasite infection on the host...
  33. ncbi request reprint Brainstem axonal degeneration in mice with deletion of selenoprotein p
    William M Valentine
    Department of Pathology, Vanderbilt University School of Medicine, Vanderbilt University Medical Center, Nashville, Tennessee 37232 2561, USA
    Toxicol Pathol 33:570-6. 2005
    ....
  34. ncbi request reprint Selenoprotein P: an extracellular protein with unique physical characteristics and a role in selenium homeostasis
    Raymond F Burk
    Division of Gastroenterology, Department of Medicine and Center in Molecular Toxicology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    Annu Rev Nutr 25:215-35. 2005
    ....
  35. ncbi request reprint Effectiveness of selenium supplements in a low-selenium area of China
    Yiming Xia
    Institute of Nutrition and Food Hygiene, Chinese Academy of Preventive Medicine, Beijing, China
    Am J Clin Nutr 81:829-34. 2005
    ..Although there is no evidence of selenium deficiency in the United States, people in many other areas of the world are selenium deficient, with the consequence that they are unable to express their selenoproteins fully...
  36. ncbi request reprint Thioredoxin reductase reduces lipid hydroperoxides and spares alpha-tocopherol
    James M May
    Department of Medicine, Vanderbilt University School of Medicine, 715 Preston Research Building II, 2220 Pierce Avenue, Nashville, TN 37232 6303, USA
    Biochem Biophys Res Commun 292:45-9. 2002
    ..Thioredoxin reductase also decreased F(2)-isoprostanes in ghosts oxidized by ferricyanide, suggesting that its ability to spare alpha-tocopherol relates to reduction of lipid hydroperoxides...
  37. ncbi request reprint Deletion of selenoprotein P alters distribution of selenium in the mouse
    Kristina E Hill
    Division of Gastroenterology, Department of Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    J Biol Chem 278:13640-6. 2003
    ..Further, they indicate that transport forms of selenium other than Se-P exist because selenium levels of all tissues except testis responded to increases of dietary selenium in Sepp(-/-) mice...
  38. ncbi request reprint Loss of activity of the selenoenzyme thioredoxin reductase causes induction of hepatic heme oxygenase-1
    Volker Mostert
    Division of Gastroenterology, Vanderbilt University School of Medicine, Nashville, TN 37232 2279, USA
    FEBS Lett 541:85-8. 2003
    ..In the rat hepatoma cell line H4IIE, inhibition of TrxR by aurothioglucose or by 1-chloro-2,4-dinitrobenzene led to induction of HO-1. We conclude that loss of TrxR is responsible for the induction of HO-1 by selenium deficiency...
  39. ncbi request reprint Selenoprotein metabolism and function: evidence for more than one function for selenoprotein P
    Raymond F Burk
    Division of Gastroenterology, Department of Medicine and Clinical Nutrition Research Unit, Vanderbilt University School of Medicine, Nashville, TN 37232 2279, USA
    J Nutr 133:1517S-20S. 2003
    ..Thus, there is evidence for both antioxidant and selenium transport functions of selenoprotein P...
  40. ncbi request reprint Selective induction of liver parenchymal cell heme oxygenase-1 in selenium-deficient rats
    Volker Mostert
    Division of Gastroenterology, Department of Medicine and Clinical Nutrition Research Unit, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    Biol Chem 384:681-7. 2003
    ..We speculate that selenium deficiency affects hepatic antioxidant selenoproteins, resulting in an up-regulation of HO-1...
  41. ncbi request reprint Loss of selenium from selenoproteins: conversion of selenocysteine to dehydroalanine in vitro
    Shuguang Ma
    Mass Spectrometry Research Center, Vanderbilt University, Nashville, Tennessee 37232 6400, USA
    J Am Soc Mass Spectrom 14:593-600. 2003
    ..The loss of selenium from selenoproteins is probably through the mechanism of oxidation of selenocysteine residue to selenoxide followed by syn-beta-elimination of selenenic acid during sample processing...
  42. ncbi request reprint Combined deficiency of vitamins E and C causes paralysis and death in guinea pigs
    Kristina E Hill
    Division of Gastroenterology, Department of Medicine, Vanderbilt University School of Medicine, Nashville, TN, USA
    Am J Clin Nutr 77:1484-8. 2003
    ..On the basis of in vitro studies, the antioxidant nutrients vitamins E and C are postulated to interact in vivo...
  43. ncbi request reprint Mass spectrometric identification of N- and O-glycosylation sites of full-length rat selenoprotein P and determination of selenide-sulfide and disulfide linkages in the shortest isoform
    Shuguang Ma
    Mass Spectrometry Research Center, Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    Biochemistry 42:9703-11. 2003
    ..The disulfide formed by Cys153-Cys156 also has some characteristics of a redox active pair...
  44. ncbi request reprint Neurological dysfunction occurs in mice with targeted deletion of the selenoprotein P gene
    Kristina E Hill
    Division of Gastroenterology and Clinical Nutrition Research Unit, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    J Nutr 134:157-61. 2004
    ..Both the absence of selenoprotein P and a low dietary selenium supply are necessary for the dysfunction to occur, indicating that selenoprotein P and at least one other form of selenium supply the element to the brain...
  45. ncbi request reprint Sequential development of flagellar defects in spermatids and epididymal spermatozoa of selenium-deficient rats
    Gary E Olson
    Department of Cell and Developmental Biology, Department of Medicine, Vanderbilt University, Nashville, Tennessee 37232, USA
    Reproduction 127:335-42. 2004
    ..These data suggest that the known loss of male fertility in selenium deficiency results from the sequential development of sperm defects expressed during both spermiogenesis and maturation in the epididymis...
  46. ncbi request reprint Mass spectrometric determination of selenenylsulfide linkages in rat selenoprotein P
    Shuguang Ma
    Mass Spectrometry Research Center, Vanderbilt University, Nashville, Tennessee 37232, USA
    J Mass Spectrom 40:400-4. 2005
    ..It is concluded that selenoprotein P contains these two selenenylsulfide bonds...
  47. ncbi request reprint Selenoprotein P is required for mouse sperm development
    Gary E Olson
    Department of Cell and Developmental Biology, Vanderbilt University, Nashville, Tennessee 37232, USA
    Biol Reprod 73:201-11. 2005
    ..These data demonstrate that SEPP1 is required for development of functional spermatozoa and indicate that it is an essential component of the selenium delivery pathway for developing germ cells...
  48. ncbi request reprint Mass spectrometric characterization of full-length rat selenoprotein P and three isoforms shortened at the C terminus. Evidence that three UGA codons in the mRNA open reading frame have alternative functions of specifying selenocysteine insertion or trans
    Shuguang Ma
    Mass Spectrometry Research Center, Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232 2279, USA
    J Biol Chem 277:12749-54. 2002
    ....
  49. pmc Plasma selenium measurements in subjects from areas with contrasting gastric cancer risks in Colombia
    M Constanza Camargo
    Department of Medicine, School of Medicine, Division of Gastroenterology, Hepatology and Nutrition, Vanderbilt University, Nashville, Tennessee 37232 0253, USA
    Arch Med Res 39:443-51. 2008
    ..This pilot study aimed to determine and compare selenium status in two Colombian populations with different gastric cancer risks: a high-risk area in the volcanic region of the Andes Mountains and a low-risk area on the Pacific coast...