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Genomes and Genes | Olivier BoutaudSummaryAffiliation: Vanderbilt University Country: USA Publications
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Publications
Characterization of the lysyl adducts of prostaglandin H-synthases that are derived from oxygenation of arachidonic acidO Boutaud
Department of Pharmacology, Mass Spectrometry Research Center, Vanderbilt University, Nashville, Tennessee 37232 6602, USA
Biochemistry 40:6948-55. 2001..The reactivity of the PGH-synthase adducts themselves is demonstrated by the formation of intermolecular cross-links...
Determinants of the cellular specificity of acetaminophen as an inhibitor of prostaglandin H(2) synthasesOlivier Boutaud
Department of Medicine, Vanderbilt University, Nashville, TN 37232 6602, USA
Proc Natl Acad Sci U S A 99:7130-5. 2002..Together these findings support the hypothesis that the clinical action of acetaminophen is mediated by inhibition of PGHS activity, and that hydroperoxide concentration contributes to its cellular selectivity...- PGH2-derived levuglandin adducts increase the neurotoxicity of amyloid beta1-42Olivier Boutaud
Department of Pharmacology, Vanderbilt University, Nashville 37232 6602, Tennessee, USA
J Neurochem 96:917-23. 2006.... 
Study of inhibitors of the PGH synthases for which potency is regulated by the redox state of the enzymesOlivier Boutaud
Department of Pharmacology, Vanderbilt University, Nashville, TN, USA
Methods Mol Biol 644:67-90. 2010..Accordingly, analysis of the action of these inhibitors requires consideration of assay conditions that take into account the factors that control hydroperoxide concentrations, both in studies with purified enzymes and in cells...- Tristetraprolin binds to the COX-2 mRNA 3' untranslated region in cancer cellsOlivier Boutaud
Division of Clinical Pharmacology, Vanderbilt University, Nashville, TN 37232-6602, USA
Adv Exp Med Biol 525:157-60. 2003 - Measurement of chronic oxidative and inflammatory stress by quantification of isoketal/levuglandin gamma-ketoaldehyde protein adducts using liquid chromatography tandem mass spectrometrySean S Davies
Department of Pharmacology, Vanderbilt University, Nashville, Tennessee 27232 6602, USA
Nat Protoc 2:2079-91. 2007..IsoK/LG-lysyl-lactam adducts are measured by liquid chromatography tandem mass spectrometry after proteolytic digestion of extracted proteins, solid-phase extraction and preparative HPLC... - Pyridoxamine analogues scavenge lipid-derived gamma-ketoaldehydes and protect against H2O2-mediated cytotoxicitySean S Davies
Departments of Pharmacology, Vanderbilt University, Nashville, Tennessee 37232, USA
Biochemistry 45:15756-67. 2006.... - Characterization of bis(levuglandinyl) urea derivatives as products of the reaction between prostaglandin H2 and arginineIrene Zagol-Ikapitte
Department of Pharmacology, Vanderbilt University, Nashville, Tennessee 37232-6602, USA
Biochemistry 43:5503-10. 2004..The soluble characteristic of these molecules provides a potential strategy for development of biological markers of lipid modification of proteins following cyclooxygenase activity or lipid peroxidation... - Levuglandinyl adducts of proteins are formed via a prostaglandin H2 synthase-dependent pathway after platelet activationOlivier Boutaud
Department of Pharmacology, Vanderbilt University, Nashville, Tennessee 37232 6602, USA
J Biol Chem 278:16926-8. 2003..They also demonstrate that a physiological stimulus is sufficient to lead to the lipid modification of proteins through the levuglandin pathway in human platelets... - Tristetraprolin binds to the 3'-untranslated region of cyclooxygenase-2 mRNA. A polyadenylation variant in a cancer cell line lacks the binding siteHitoshi Sawaoka
Department of Pharmacology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 6602, USA
J Biol Chem 278:13928-35. 2003..However, the truncated transcript escaped tristetraprolin binding and downregulation... - Protease-activated receptor signaling in platelets activates cytosolic phospholipase A2α differently for cyclooxygenase-1 and 12-lipoxygenase catalysisMichael Holinstat
Cardeza Foundation for Hematologic Research, Department of Medicine, Thomas Jefferson University, 1015 Walnut Street, Philadelphia, PA 19107, USA
Arterioscler Thromb Vasc Biol 31:435-42. 2011..We evaluated signaling via the human platelet thrombin receptors, protease-activated receptor (PAR) 1 and PAR4, to the activation of cPLA(2α), which provides a substrate for the biosynthesis of TxA(2) and 12-HETE... - Characterization of scavengers of gamma-ketoaldehydes that do not inhibit prostaglandin biosynthesisIrene Zagol-Ikapitte
Departments of Pharmacology, Pathology, and Medicine, Vanderbilt University, Nashville, Tennessee 37232 6602, USA
Chem Res Toxicol 23:240-50. 2010.... - Suboptimal inhibition of platelet cyclooxygenase-1 by aspirin in metabolic syndromeJames P Smith
Department of Medicine, Vanderbilt University Medical Center, Nashville, TN, USA
Hypertension 59:719-25. 2012..In conclusion, metabolic syndrome, which places patients at high risk for thrombotic cardiovascular events, strongly and uniquely associates with less effective inhibition of platelet COX-1 by aspirin... - Protein kinase C regulation of 12-lipoxygenase-mediated human platelet activationJennifer Yeung
Department of Medicine, Cardeza Foundation for Hematologic Research, Thomas Jefferson University, Philadelphia, PA 19107, USA
Mol Pharmacol 81:420-30. 2012..These studies support an essential role for PKC downstream of 12-LOX activation in human platelets and suggest 12-LOX as a possible target for antiplatelet therapy... - Pyridine and pyrimidine analogs of acetaminophen as inhibitors of lipid peroxidation and cyclooxygenase and lipoxygenase catalysisTae Gyu Nam
Department of Chemistry, Vanderbilt University, Nashville, TN 37235, USA
Org Biomol Chem 7:5103-12. 2009..The pyrimidinols, however, were up to 17-fold more reactive to peroxyl radicals and up to 25-fold better inhibitors of prostaglandin biosynthesis than ApAP, with similar cytotoxicities to HepG2 cells at high levels of exposure... - Prostaglandin H(2)-derived adducts of proteins correlate with Alzheimer's disease severityIrene Zagol-Ikapitte
Department of Pharmacology, Vanderbilt University, Nashville, TN, USA
J Neurochem 94:1140-5. 2005..The magnitude of the increase correlates with the pathological evidence of severity... - Acetaminophen inhibits hemoprotein-catalyzed lipid peroxidation and attenuates rhabdomyolysis-induced renal failureOlivier Boutaud
Department of Pharmacology, Medicine, and Chemistry, Vanderbilt University, Nashville, TN 37232, USA
Proc Natl Acad Sci U S A 107:2699-704. 2010..These findings also provide a hypothesis for potential therapeutic applications for acetaminophen in diseases involving hemoprotein-mediated oxidative injury... - Mechanism-based therapeutic approaches to rhabdomyolysis-induced renal failureOlivier Boutaud
Department of Pharmacology, Vanderbilt University Medical Center, Nashville, TN 37232, USA
Free Radic Biol Med 51:1062-7. 2011.... - Prostaglandin H2 (PGH2) accelerates formation of amyloid beta1-42 oligomersOlivier Boutaud
Department of Pharmacology, Center for Molecular Neuroscience, Vanderbilt University, Nashville, Tennessee, USA
J Neurochem 82:1003-6. 2002..This is associated with the formation of levuglandin adducts of the peptide. These findings provide the molecular basis for a hypothesis linking cyclooxygenase activity to the formation of oligomers of amyloid beta... - Acetylation of prostaglandin H2 synthases by aspirin is inhibited by redox cycling of the peroxidaseManju Bala
Department of Pharmacology, Vanderbilt University, 2200 Pierce Avenue, 514 RRB, Nashville, TN 37232 6602, United States
Biochem Pharmacol 75:1472-81. 2008..0+/-0.9 microM, respectively). Together, these findings indicate that acetylation of the PGHSs by aspirin is regulated by the catalytic activity of the peroxidase, which yields a higher oxidative state of the enzyme... - Acetaminophen inhibits cytochrome c redox cycling induced lipid peroxidationHuiyong Yin
Department of Medicine, Division of Clinical Pharmacology, Vanderbilt University School of Medicine, TN 37232, USA
Biochem Biophys Res Commun 423:224-8. 2012..Our study also provides a potentially novel pharmacological approach for inhibiting the cascade of events that can result from redox cycling of cyt c... - On-tissue chemical derivatization of 3-methoxysalicylamine for MALDI-imaging mass spectrometryAlmary Chacon
Department of Pharmacology, Vanderbilt University, Nashville, TN 37232 6602, USA
J Mass Spectrom 46:840-6. 2011..These results provide proof-of-concept that on-tissue chemical derivatization can be used to improve detection of a small organic molecule by MALDI-IMS... - Modification of proteins by isoketal-containing oxidized phospholipidsCynthia J Brame
Departments of Pharmacology and Medicine, Vanderbilt University, Nashville, Tennessee 27232-6602, USA
J Biol Chem 279:13447-51. 2004.... - Cyclooxygenase-2-dependent prostacyclin formation is regulated by low density lipoprotein cholesterol in vitroLayton Harris Smith
Department of Pharmacology, Vanderbilt University Medical Center, Nashville, Tenn 37232, USA
Arterioscler Thromb Vasc Biol 22:983-8. 2002.... - Cyclooxygenase-dependent lipid-modification of brain proteinsOlivier Boutaud
Department of Pharmacology, Vanderbilt University, Nashville, TN 37232 6602, USA
Brain Pathol 15:139-42. 2005..In this review, we discuss the cyclooxygenase-dependent lipid-modification of proteins by levuglandins in vitro, in cells in culture and in vivo in transgenic mice over-expressing COX in the brain... - Inherited human cPLA(2alpha) deficiency is associated with impaired eicosanoid biosynthesis, small intestinal ulceration, and platelet dysfunctionDavid H Adler
Department of Medicine, Division of Clinical Pharmacology, Vanderbilt University Medical Center, Nashville, Tennessee 37232 6602, USA
J Clin Invest 118:2121-31. 2008..These findings characterize what we believe is a novel inherited deficiency of cPLA2... - Inhibition of prostaglandin H2 synthases by salicylate is dependent on the oxidative state of the enzymesDavid M Aronoff
Department of Medicine, Vanderbilt University, Nashville, Tennessee 37232, USA
J Pharmacol Exp Ther 304:589-95. 2003..These results are consistent with an inhibitory action of salicylate at the PGHS-cyclooxygenase site that is dependent on the PGHS-peroxidase activity... - Effects of reactive gamma-ketoaldehydes formed by the isoprostane pathway (isoketals) and cyclooxygenase pathway (levuglandins) on proteasome functionSean S Davies
Department of Pharmacology, Vanderbilt University, Nashville, Tennessee 27232-6602, USA
FASEB J 16:715-7. 2002..These findings suggest that IsoKs/NeuroKs/LGs can inhibit proteasome activity and, if overproduced, may have relevance to the pathogenesis of neurodegenerative diseases... - Characterization of covalent adducts of nucleosides and DNA formed by reaction with levuglandinErica J Carrier
Department of Medicine, Vanderbilt University, Nashville, Tennessee 37232, USA
Biochemistry 48:10775-81. 2009..These results describe the first structure of levuglandinyl-DNA adducts and provide the tools with which to evaluate the potential for LG-DNA adduct formation in vivo... - Primate-specific evolution of noncoding element insertion into PLA2G4C and human preterm birthJevon Plunkett
Department of Pediatrics, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
BMC Med Genomics 3:62. 2010..We hypothesize that higher primate-specific gene evolution may lead to these differences and target genes involved in human preterm birth, an area of global health significance... - Human colorectal cancer cells efficiently conjugate the cyclopentenone prostaglandin, prostaglandin J(2), to glutathioneBrian Cox
Department of Medicine, Vanderbilt University School of Medicine, Nashville, TN 37232-6602, USA
Biochim Biophys Acta 1584:37-45. 2002..Approximately 70% of the PGJ(2) added to HCA-7 cells was esterifed to GSH after 2 h of incubation, suggesting this pathway represents the major route of metabolic disposition of PGJ(2) in HCA-7 cells... - Determination of 3-methoxysalicylamine levels in mouse plasma and tissue by liquid chromatography-tandem mass spectrometry: application to in vivo pharmacokinetics studiesIrene Zagol-Ikapitte
Department of Pharmacology, Vanderbilt University, Nashville, TN 37232 6602, USA
J Chromatogr B Analyt Technol Biomed Life Sci 879:1098-104. 2011..In addition, we used this assay to analyze the pharmacokinetics of 3-MoSA in mouse plasma and tissues following both intraperitoneal and oral administration, providing new information regarding the distribution of this compound in vivo... - Functional characterization of mutations in inherited human cPLA₂ deficiencyKathleen A Reed
Department of Medicine, Vanderbilt University, School of Medicine, Nashville, Tennessee 37232 6602, United States
Biochemistry 50:1731-8. 2011..Our results define how the mutations negatively influence cPLA(2)α function and explain the inability of the proband to release arachidonic acid for eicosanoid production... - PGE2 decreases reactivity of human platelets by activating EP2 and EP4James P Smith
Department of Medicine, Vanderbilt University, Nashville, TN 37232, USA
Thromb Res 126:e23-9. 2010..Studies in mice and humans suggest that prostaglandin E2 (PGE2) regulates platelet activation. In mice, activation of the PGE2 receptor subtype 3 (EP3) promotes thrombosis, but the significance of EP3 in humans is less well understood... - Genetic screening in C. elegans identifies rho-GTPase activating protein 6 as novel HERG regulatorFranck Potet
Department of Anesthesiology, Vanderbilt University, Nashville, TN 37232, USA
J Mol Cell Cardiol 46:257-67. 2009..These data link a rhoGAP-activated PLC pathway to HERG membrane expression and implicate this family of proteins as candidate genes in disorders involving HERG... - New insights into the mechanism of action of acetaminophen: Its clinical pharmacologic characteristics reflect its inhibition of the two prostaglandin H2 synthasesDavid M Aronoff
Division of Infectious Diseases, Department of Internal Medicine, University of Michigan Health System, Ann Arbor, Mich, USA
Clin Pharmacol Ther 79:9-19. 2006 - 11,12-epoxyeicosatrienoic acid attenuates synthesis of prostaglandin E2 in rat monocytes stimulated with lipopolysaccharideWieslaw Kozak
Department of Physiology, Medical College of Georgia, Augusta, Georgia 30912, USA
Exp Biol Med (Maywood) 228:786-94. 2003..These data support the hypothesis that epoxygenase-derived AA metabolites constitute a negative feedback on the enhanced synthesis of prostaglandins upon inflammation... - Inhibition of prostaglandin H2 synthases by salicylate is dependent on the oxidative state of the enzymesDavid M Aronoff
Division of Infectious Diseases and Pulmonary/Critical Care Medicine, 6301 MSRB III, Ann Arbor, MI 48109-0642, USA
Adv Exp Med Biol 525:125-8. 2003