Olivier Boutaud

Summary

Affiliation: Vanderbilt University
Country: USA

Publications

  1. ncbi request reprint Characterization of the lysyl adducts of prostaglandin H-synthases that are derived from oxygenation of arachidonic acid
    O Boutaud
    Department of Pharmacology, Mass Spectrometry Research Center, Vanderbilt University, Nashville, Tennessee 37232 6602, USA
    Biochemistry 40:6948-55. 2001
  2. pmc PGH2-derived levuglandin adducts increase the neurotoxicity of amyloid beta1-42
    Olivier Boutaud
    Department of Pharmacology, Vanderbilt University, Nashville 37232 6602, Tennessee, USA
    J Neurochem 96:917-23. 2006
  3. pmc Determinants of the cellular specificity of acetaminophen as an inhibitor of prostaglandin H(2) synthases
    Olivier Boutaud
    Department of Medicine, Vanderbilt University, Nashville, TN 37232 6602, USA
    Proc Natl Acad Sci U S A 99:7130-5. 2002
  4. ncbi request reprint Tristetraprolin binds to the COX-2 mRNA 3' untranslated region in cancer cells
    Olivier Boutaud
    Division of Clinical Pharmacology, Vanderbilt University, Nashville, TN 37232 6602, USA
    Adv Exp Med Biol 525:157-60. 2003
  5. ncbi request reprint Study of inhibitors of the PGH synthases for which potency is regulated by the redox state of the enzymes
    Olivier Boutaud
    Department of Pharmacology, Vanderbilt University, Nashville, TN, USA
    Methods Mol Biol 644:67-90. 2010
  6. ncbi request reprint Measurement of chronic oxidative and inflammatory stress by quantification of isoketal/levuglandin gamma-ketoaldehyde protein adducts using liquid chromatography tandem mass spectrometry
    Sean S Davies
    Department of Pharmacology, Vanderbilt University, Nashville, Tennessee 27232 6602, USA
    Nat Protoc 2:2079-91. 2007
  7. pmc Pyridoxamine analogues scavenge lipid-derived gamma-ketoaldehydes and protect against H2O2-mediated cytotoxicity
    Sean S Davies
    Departments of Pharmacology, Vanderbilt University, Nashville, Tennessee 37232, USA
    Biochemistry 45:15756-67. 2006
  8. ncbi request reprint Characterization of bis(levuglandinyl) urea derivatives as products of the reaction between prostaglandin H2 and arginine
    Irene Zagol-Ikapitte
    Department of Pharmacology, Vanderbilt University, Nashville, Tennessee 37232 6602, USA
    Biochemistry 43:5503-10. 2004
  9. pmc Characterization of scavengers of gamma-ketoaldehydes that do not inhibit prostaglandin biosynthesis
    Irene Zagol-Ikapitte
    Departments of Pharmacology, Pathology, and Medicine, Vanderbilt University, Nashville, Tennessee 37232 6602, USA
    Chem Res Toxicol 23:240-50. 2010
  10. pmc Protease-activated receptor signaling in platelets activates cytosolic phospholipase A2α differently for cyclooxygenase-1 and 12-lipoxygenase catalysis
    Michael Holinstat
    Cardeza Foundation for Hematologic Research, Department of Medicine, Thomas Jefferson University, 1015 Walnut Street, Philadelphia, PA 19107, USA
    Arterioscler Thromb Vasc Biol 31:435-42. 2011

Collaborators

Detail Information

Publications40

  1. ncbi request reprint Characterization of the lysyl adducts of prostaglandin H-synthases that are derived from oxygenation of arachidonic acid
    O Boutaud
    Department of Pharmacology, Mass Spectrometry Research Center, Vanderbilt University, Nashville, Tennessee 37232 6602, USA
    Biochemistry 40:6948-55. 2001
    ..The reactivity of the PGH-synthase adducts themselves is demonstrated by the formation of intermolecular cross-links...
  2. pmc PGH2-derived levuglandin adducts increase the neurotoxicity of amyloid beta1-42
    Olivier Boutaud
    Department of Pharmacology, Vanderbilt University, Nashville 37232 6602, Tennessee, USA
    J Neurochem 96:917-23. 2006
    ....
  3. pmc Determinants of the cellular specificity of acetaminophen as an inhibitor of prostaglandin H(2) synthases
    Olivier Boutaud
    Department of Medicine, Vanderbilt University, Nashville, TN 37232 6602, USA
    Proc Natl Acad Sci U S A 99:7130-5. 2002
    ..Together these findings support the hypothesis that the clinical action of acetaminophen is mediated by inhibition of PGHS activity, and that hydroperoxide concentration contributes to its cellular selectivity...
  4. ncbi request reprint Tristetraprolin binds to the COX-2 mRNA 3' untranslated region in cancer cells
    Olivier Boutaud
    Division of Clinical Pharmacology, Vanderbilt University, Nashville, TN 37232 6602, USA
    Adv Exp Med Biol 525:157-60. 2003
  5. ncbi request reprint Study of inhibitors of the PGH synthases for which potency is regulated by the redox state of the enzymes
    Olivier Boutaud
    Department of Pharmacology, Vanderbilt University, Nashville, TN, USA
    Methods Mol Biol 644:67-90. 2010
    ..Accordingly, analysis of the action of these inhibitors requires consideration of assay conditions that take into account the factors that control hydroperoxide concentrations, both in studies with purified enzymes and in cells...
  6. ncbi request reprint Measurement of chronic oxidative and inflammatory stress by quantification of isoketal/levuglandin gamma-ketoaldehyde protein adducts using liquid chromatography tandem mass spectrometry
    Sean S Davies
    Department of Pharmacology, Vanderbilt University, Nashville, Tennessee 27232 6602, USA
    Nat Protoc 2:2079-91. 2007
    ..IsoK/LG-lysyl-lactam adducts are measured by liquid chromatography tandem mass spectrometry after proteolytic digestion of extracted proteins, solid-phase extraction and preparative HPLC...
  7. pmc Pyridoxamine analogues scavenge lipid-derived gamma-ketoaldehydes and protect against H2O2-mediated cytotoxicity
    Sean S Davies
    Departments of Pharmacology, Vanderbilt University, Nashville, Tennessee 37232, USA
    Biochemistry 45:15756-67. 2006
    ....
  8. ncbi request reprint Characterization of bis(levuglandinyl) urea derivatives as products of the reaction between prostaglandin H2 and arginine
    Irene Zagol-Ikapitte
    Department of Pharmacology, Vanderbilt University, Nashville, Tennessee 37232 6602, USA
    Biochemistry 43:5503-10. 2004
    ..The soluble characteristic of these molecules provides a potential strategy for development of biological markers of lipid modification of proteins following cyclooxygenase activity or lipid peroxidation...
  9. pmc Characterization of scavengers of gamma-ketoaldehydes that do not inhibit prostaglandin biosynthesis
    Irene Zagol-Ikapitte
    Departments of Pharmacology, Pathology, and Medicine, Vanderbilt University, Nashville, Tennessee 37232 6602, USA
    Chem Res Toxicol 23:240-50. 2010
    ....
  10. pmc Protease-activated receptor signaling in platelets activates cytosolic phospholipase A2α differently for cyclooxygenase-1 and 12-lipoxygenase catalysis
    Michael Holinstat
    Cardeza Foundation for Hematologic Research, Department of Medicine, Thomas Jefferson University, 1015 Walnut Street, Philadelphia, PA 19107, USA
    Arterioscler Thromb Vasc Biol 31:435-42. 2011
    ..We evaluated signaling via the human platelet thrombin receptors, protease-activated receptor (PAR) 1 and PAR4, to the activation of cPLA(2α), which provides a substrate for the biosynthesis of TxA(2) and 12-HETE...
  11. ncbi request reprint Levuglandinyl adducts of proteins are formed via a prostaglandin H2 synthase-dependent pathway after platelet activation
    Olivier Boutaud
    Department of Pharmacology, Vanderbilt University, Nashville, Tennessee 37232 6602, USA
    J Biol Chem 278:16926-8. 2003
    ..They also demonstrate that a physiological stimulus is sufficient to lead to the lipid modification of proteins through the levuglandin pathway in human platelets...
  12. ncbi request reprint Tristetraprolin binds to the 3'-untranslated region of cyclooxygenase-2 mRNA. A polyadenylation variant in a cancer cell line lacks the binding site
    Hitoshi Sawaoka
    Department of Pharmacology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 6602, USA
    J Biol Chem 278:13928-35. 2003
    ..However, the truncated transcript escaped tristetraprolin binding and downregulation...
  13. pmc Levuglandin forms adducts with histone h4 in a cyclooxygenase-2-dependent manner, altering its interaction with DNA
    Erica J Carrier
    Departments of Pharmacology, Pathology, and Medicine, Vanderbilt University, Nashville, Tennessee 37232, United States
    Biochemistry 53:2436-41. 2014
    ..These data demonstrate that COX-2 activity can cause histone adduction and loosening of the nucleosome complex, which could lead to altered transcription and contribute to carcinogenesis. ..
  14. pmc Suboptimal inhibition of platelet cyclooxygenase-1 by aspirin in metabolic syndrome
    James P Smith
    Department of Medicine, Vanderbilt University Medical Center, Nashville, TN, USA
    Hypertension 59:719-25. 2012
    ..In conclusion, metabolic syndrome, which places patients at high risk for thrombotic cardiovascular events, strongly and uniquely associates with less effective inhibition of platelet COX-1 by aspirin...
  15. pmc Protein kinase C regulation of 12-lipoxygenase-mediated human platelet activation
    Jennifer Yeung
    Department of Medicine, Cardeza Foundation for Hematologic Research, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Mol Pharmacol 81:420-30. 2012
    ..These studies support an essential role for PKC downstream of 12-LOX activation in human platelets and suggest 12-LOX as a possible target for antiplatelet therapy...
  16. ncbi request reprint Prostaglandin H(2)-derived adducts of proteins correlate with Alzheimer's disease severity
    Irene Zagol-Ikapitte
    Department of Pharmacology, Vanderbilt University, Nashville, TN, USA
    J Neurochem 94:1140-5. 2005
    ..The magnitude of the increase correlates with the pathological evidence of severity...
  17. pmc Acetaminophen inhibits hemoprotein-catalyzed lipid peroxidation and attenuates rhabdomyolysis-induced renal failure
    Olivier Boutaud
    Department of Pharmacology, Medicine, and Chemistry, Vanderbilt University, Nashville, TN 37232, USA
    Proc Natl Acad Sci U S A 107:2699-704. 2010
    ..These findings also provide a hypothesis for potential therapeutic applications for acetaminophen in diseases involving hemoprotein-mediated oxidative injury...
  18. pmc Mechanism-based therapeutic approaches to rhabdomyolysis-induced renal failure
    Olivier Boutaud
    Department of Pharmacology, Vanderbilt University Medical Center, Nashville, TN 37232, USA
    Free Radic Biol Med 51:1062-7. 2011
    ....
  19. pmc Pyridine and pyrimidine analogs of acetaminophen as inhibitors of lipid peroxidation and cyclooxygenase and lipoxygenase catalysis
    Tae Gyu Nam
    Department of Chemistry, Vanderbilt University, Nashville, TN 37235, USA
    Org Biomol Chem 7:5103-12. 2009
    ..The pyrimidinols, however, were up to 17-fold more reactive to peroxyl radicals and up to 25-fold better inhibitors of prostaglandin biosynthesis than ApAP, with similar cytotoxicities to HepG2 cells at high levels of exposure...
  20. pmc Acetylation of prostaglandin H2 synthases by aspirin is inhibited by redox cycling of the peroxidase
    Manju Bala
    Department of Pharmacology, Vanderbilt University, 2200 Pierce Avenue, 514 RRB, Nashville, TN 37232 6602, United States
    Biochem Pharmacol 75:1472-81. 2008
    ..0+/-0.9 microM, respectively). Together, these findings indicate that acetylation of the PGHSs by aspirin is regulated by the catalytic activity of the peroxidase, which yields a higher oxidative state of the enzyme...
  21. ncbi request reprint Prostaglandin H2 (PGH2) accelerates formation of amyloid beta1-42 oligomers
    Olivier Boutaud
    Department of Pharmacology, Center for Molecular Neuroscience, Vanderbilt University, Nashville, Tennessee, USA
    J Neurochem 82:1003-6. 2002
    ..This is associated with the formation of levuglandin adducts of the peptide. These findings provide the molecular basis for a hypothesis linking cyclooxygenase activity to the formation of oligomers of amyloid beta...
  22. pmc Acetaminophen inhibits cytochrome c redox cycling induced lipid peroxidation
    Huiyong Yin
    Department of Medicine, Division of Clinical Pharmacology, Vanderbilt University School of Medicine, TN 37232, USA
    Biochem Biophys Res Commun 423:224-8. 2012
    ..Our study also provides a potentially novel pharmacological approach for inhibiting the cascade of events that can result from redox cycling of cyt c...
  23. pmc On-tissue chemical derivatization of 3-methoxysalicylamine for MALDI-imaging mass spectrometry
    Almary Chacon
    Department of Pharmacology, Vanderbilt University, Nashville, TN 37232 6602, USA
    J Mass Spectrom 46:840-6. 2011
    ..These results provide proof-of-concept that on-tissue chemical derivatization can be used to improve detection of a small organic molecule by MALDI-IMS...
  24. ncbi request reprint Modification of proteins by isoketal-containing oxidized phospholipids
    Cynthia J Brame
    Departments of Pharmacology and Medicine, Vanderbilt University, Nashville, Tennessee 27232 6602, USA
    J Biol Chem 279:13447-51. 2004
    ....
  25. pmc Suppression of Alzheimer-associated inflammation by microglial prostaglandin-E2 EP4 receptor signaling
    Nathaniel S Woodling
    Department of Neurology and Neurological Sciences, Stanford University School of Medicine, Stanford, California 94305, Neurosciences Graduate Program, Stanford University, Stanford, California 94305, and Department of Pharmacology, Vanderbilt University, Nashville, Tennessee 37232
    J Neurosci 34:5882-94. 2014
    ....
  26. ncbi request reprint Cyclooxygenase-dependent lipid-modification of brain proteins
    Olivier Boutaud
    Department of Pharmacology, Vanderbilt University, Nashville, TN 37232 6602, USA
    Brain Pathol 15:139-42. 2005
    ..In this review, we discuss the cyclooxygenase-dependent lipid-modification of proteins by levuglandins in vitro, in cells in culture and in vivo in transgenic mice over-expressing COX in the brain...
  27. ncbi request reprint Cyclooxygenase-2-dependent prostacyclin formation is regulated by low density lipoprotein cholesterol in vitro
    Layton Harris Smith
    Department of Pharmacology, Vanderbilt University Medical Center, Nashville, Tenn 37232, USA
    Arterioscler Thromb Vasc Biol 22:983-8. 2002
    ....
  28. ncbi request reprint Effects of reactive gamma-ketoaldehydes formed by the isoprostane pathway (isoketals) and cyclooxygenase pathway (levuglandins) on proteasome function
    Sean S Davies
    Department of Pharmacology, Vanderbilt University, Nashville, Tennessee 27232 6602, USA
    FASEB J 16:715-7. 2002
    ..These findings suggest that IsoKs/NeuroKs/LGs can inhibit proteasome activity and, if overproduced, may have relevance to the pathogenesis of neurodegenerative diseases...
  29. pmc Inherited human cPLA(2alpha) deficiency is associated with impaired eicosanoid biosynthesis, small intestinal ulceration, and platelet dysfunction
    David H Adler
    Department of Medicine, Division of Clinical Pharmacology, Vanderbilt University Medical Center, Nashville, Tennessee 37232 6602, USA
    J Clin Invest 118:2121-31. 2008
    ..These findings characterize what we believe is a novel inherited deficiency of cPLA2...
  30. ncbi request reprint Inhibition of prostaglandin H2 synthases by salicylate is dependent on the oxidative state of the enzymes
    David M Aronoff
    Department of Medicine, Vanderbilt University, Nashville, Tennessee 37232, USA
    J Pharmacol Exp Ther 304:589-95. 2003
    ..These results are consistent with an inhibitory action of salicylate at the PGHS-cyclooxygenase site that is dependent on the PGHS-peroxidase activity...
  31. pmc Characterization of covalent adducts of nucleosides and DNA formed by reaction with levuglandin
    Erica J Carrier
    Department of Medicine, Vanderbilt University, Nashville, Tennessee 37232, USA
    Biochemistry 48:10775-81. 2009
    ..These results describe the first structure of levuglandinyl-DNA adducts and provide the tools with which to evaluate the potential for LG-DNA adduct formation in vivo...
  32. pmc Primate-specific evolution of noncoding element insertion into PLA2G4C and human preterm birth
    Jevon Plunkett
    Department of Pediatrics, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    BMC Med Genomics 3:62. 2010
    ..We hypothesize that higher primate-specific gene evolution may lead to these differences and target genes involved in human preterm birth, an area of global health significance...
  33. ncbi request reprint Human colorectal cancer cells efficiently conjugate the cyclopentenone prostaglandin, prostaglandin J(2), to glutathione
    Brian Cox
    Department of Medicine, Vanderbilt University School of Medicine, Nashville, TN 37232 6602, USA
    Biochim Biophys Acta 1584:37-45. 2002
    ..Approximately 70% of the PGJ(2) added to HCA-7 cells was esterifed to GSH after 2 h of incubation, suggesting this pathway represents the major route of metabolic disposition of PGJ(2) in HCA-7 cells...
  34. pmc Determination of 3-methoxysalicylamine levels in mouse plasma and tissue by liquid chromatography-tandem mass spectrometry: application to in vivo pharmacokinetics studies
    Irene Zagol-Ikapitte
    Department of Pharmacology, Vanderbilt University, Nashville, TN 37232 6602, USA
    J Chromatogr B Analyt Technol Biomed Life Sci 879:1098-104. 2011
    ..In addition, we used this assay to analyze the pharmacokinetics of 3-MoSA in mouse plasma and tissues following both intraperitoneal and oral administration, providing new information regarding the distribution of this compound in vivo...
  35. pmc Functional characterization of mutations in inherited human cPLA₂ deficiency
    Kathleen A Reed
    Department of Medicine, Vanderbilt University, School of Medicine, Nashville, Tennessee 37232 6602, United States
    Biochemistry 50:1731-8. 2011
    ..Our results define how the mutations negatively influence cPLA(2)α function and explain the inability of the proband to release arachidonic acid for eicosanoid production...
  36. pmc PGE2 decreases reactivity of human platelets by activating EP2 and EP4
    James P Smith
    Department of Medicine, Vanderbilt University, Nashville, TN 37232, USA
    Thromb Res 126:e23-9. 2010
    ..Studies in mice and humans suggest that prostaglandin E2 (PGE2) regulates platelet activation. In mice, activation of the PGE2 receptor subtype 3 (EP3) promotes thrombosis, but the significance of EP3 in humans is less well understood...
  37. pmc Genetic screening in C. elegans identifies rho-GTPase activating protein 6 as novel HERG regulator
    Franck Potet
    Department of Anesthesiology, Vanderbilt University, Nashville, TN 37232, USA
    J Mol Cell Cardiol 46:257-67. 2009
    ..These data link a rhoGAP-activated PLC pathway to HERG membrane expression and implicate this family of proteins as candidate genes in disorders involving HERG...
  38. ncbi request reprint 11,12-epoxyeicosatrienoic acid attenuates synthesis of prostaglandin E2 in rat monocytes stimulated with lipopolysaccharide
    Wieslaw Kozak
    Department of Physiology, Medical College of Georgia, Augusta, Georgia 30912, USA
    Exp Biol Med (Maywood) 228:786-94. 2003
    ..These data support the hypothesis that epoxygenase-derived AA metabolites constitute a negative feedback on the enhanced synthesis of prostaglandins upon inflammation...
  39. ncbi request reprint New insights into the mechanism of action of acetaminophen: Its clinical pharmacologic characteristics reflect its inhibition of the two prostaglandin H2 synthases
    David M Aronoff
    Division of Infectious Diseases, Department of Internal Medicine, University of Michigan Health System, Ann Arbor, Mich, USA
    Clin Pharmacol Ther 79:9-19. 2006
  40. ncbi request reprint Inhibition of prostaglandin H2 synthases by salicylate is dependent on the oxidative state of the enzymes
    David M Aronoff
    Division of Infectious Diseases and Pulmonary Critical Care Medicine, 6301 MSRB III, Ann Arbor, MI 48109 0642, USA
    Adv Exp Med Biol 525:125-8. 2003