Darryl Bornhop

Summary

Affiliation: Vanderbilt University
Country: USA

Publications

  1. ncbi Characterizing aptamer small molecule interactions with backscattering interferometry
    Michael N Kammer
    Department of Chemistry, Vanderbilt University, 2201 West End Avenue, Nashville, Tennessee, USA
    Analyst 139:5879-84. 2014
  2. ncbi Preclinical molecular imaging of the translocator protein (TSPO) in a metastases model based on breast cancer xenografts propagated in the murine brain
    Shelby K Wyatt
    Vanderbilt University Institute of Imaging Science, Vanderbilt University Medical Center, Nashville, TN, USA
    Curr Mol Med 12:458-66. 2012
  3. ncbi Targeted molecular imaging agents for cellular-scale bimodal imaging
    H Charles Manning
    Department of Chemistry, Vanderbilt University, Nashville, Tennessee 37232, USA
    Bioconjug Chem 15:1488-95. 2004
  4. ncbi Free-solution, label-free molecular interactions studied by back-scattering interferometry
    Darryl J Bornhop
    Department of Chemistry, Vanderbilt Institute of Chemical Biology, Vanderbilt University, VU Station B 351822, Nashville, TN 37235 1822, USA
    Science 317:1732-6. 2007
  5. ncbi Label-free molecular interaction determinations with nanoscale interferometry
    Dmitry A Markov
    Department of Chemistry, Vanderbilt University, VU Station B 351822, Nashville, Tennessee 37235 1822, USA
    J Am Chem Soc 126:16659-64. 2004
  6. pmc Free-solution interaction assay of carbonic anhydrase to its inhibitors using back-scattering interferometry
    Ereny F Morcos
    Department of Chemistry and Vanderbilt Institute of Chemical Biology, Vanderbilt University, Nashville, TN, USA
    Electrophoresis 31:3691-5. 2010
  7. pmc MBC94, a conjugable ligand for cannabinoid CB 2 receptor imaging
    Mingfeng Bai
    Department of Chemistry, Vanderbilt University, VU Station B 351822, Nashville, Tennessee 37235 1822, USA
    Bioconjug Chem 19:988-92. 2008
  8. ncbi A novel conjugable translocator protein ligand labeled with a fluorescence dye for in vitro imaging
    Mingfeng Bai
    Department of Chemistry, Vanderbilt University, VU Station B 351822 Nashville, Tennessee 37235 1822, USA
    Bioconjug Chem 18:1118-22. 2007
  9. pmc Measurement of monovalent and polyvalent carbohydrate-lectin binding by back-scattering interferometry
    Amanda Kussrow
    Department of Chemistry and Vanderbilt Institute of Chemical Biology, Vanderbilt University, 4226 Stevenson Center, Nashville, Tennessee 37235, USA
    Anal Chem 81:4889-97. 2009
  10. ncbi The potential of backscattering interferometry as an in vitro clinical diagnostic tool for the serological diagnosis of infectious disease
    Amanda Kussrow
    Department of Chemistry and Vanderbilt Institute of Chemical Biology, Vanderbilt University, Nashville, Tennessee, USA
    Analyst 135:1535-7. 2010

Research Grants

Collaborators

  • HENRY MANNING
  • V Papadopoulos
  • R D Jones
  • D L Cox
  • Hassane McHaourab
  • Mingfeng Bai
  • Shelby K Wyatt
  • Amanda Kussrow
  • Dmitry A Markov
  • Kelly Swinney
  • Michael N Kammer
  • Ereny F Morcos
  • Lynn E Samuelson
  • Joey C Latham
  • Natasha G Deane
  • Zhanling Wang
  • Mark J Morris
  • George W Jackson
  • Ian R Olmsted
  • Amanda K Kussrow
  • Khubaib Y Mapara
  • Reid C Thompson
  • Moneeb Ehtesham
  • Ronald C Ballard
  • Guobin Ma
  • Carolyn S Enders
  • Stephanie N Bailey
  • Pascal Gallant
  • Laura McIntosh
  • Arnold R Castro
  • Carolyn Enders
  • Mark L Wolfenden
  • Richard A Stein
  • Mary J Cloninger
  • Colette R Hunt
  • Madeline J Dukes
  • M G Finn
  • Jonathan D Casey
  • Eiton Kaltgrad
  • Nephi Stella
  • Michelle Sexton
  • Zeqiu Han
  • Bruce A Aronow
  • M Kay Washington
  • Bruce J Aronow
  • Robert J Coffey
  • Malena B Rone
  • A Coe Foutch
  • Stephen Dotson
  • Scott Wood

Detail Information

Publications19

  1. ncbi Characterizing aptamer small molecule interactions with backscattering interferometry
    Michael N Kammer
    Department of Chemistry, Vanderbilt University, 2201 West End Avenue, Nashville, Tennessee, USA
    Analyst 139:5879-84. 2014
    ..No immobilization or labeling chemistry was needed, expediting the assay which is also insensitive to the large relative mass difference between the interacting molecules. ..
  2. ncbi Preclinical molecular imaging of the translocator protein (TSPO) in a metastases model based on breast cancer xenografts propagated in the murine brain
    Shelby K Wyatt
    Vanderbilt University Institute of Imaging Science, Vanderbilt University Medical Center, Nashville, TN, USA
    Curr Mol Med 12:458-66. 2012
    ....
  3. ncbi Targeted molecular imaging agents for cellular-scale bimodal imaging
    H Charles Manning
    Department of Chemistry, Vanderbilt University, Nashville, Tennessee 37232, USA
    Bioconjug Chem 15:1488-95. 2004
    ....
  4. ncbi Free-solution, label-free molecular interactions studied by back-scattering interferometry
    Darryl J Bornhop
    Department of Chemistry, Vanderbilt Institute of Chemical Biology, Vanderbilt University, VU Station B 351822, Nashville, TN 37235 1822, USA
    Science 317:1732-6. 2007
    ..The high sensitivity of back-scattering interferometry and small volumes of microfluidics allowed the entire calmodulin assay to be performed with 200 picomoles of solute...
  5. ncbi Label-free molecular interaction determinations with nanoscale interferometry
    Dmitry A Markov
    Department of Chemistry, Vanderbilt University, VU Station B 351822, Nashville, Tennessee 37235 1822, USA
    J Am Chem Soc 126:16659-64. 2004
    ....
  6. pmc Free-solution interaction assay of carbonic anhydrase to its inhibitors using back-scattering interferometry
    Ereny F Morcos
    Department of Chemistry and Vanderbilt Institute of Chemical Biology, Vanderbilt University, Nashville, TN, USA
    Electrophoresis 31:3691-5. 2010
    ..BSI has the potential for reducing labor costs, sample consumption, and assay time while providing enhanced reliability over existing techniques...
  7. pmc MBC94, a conjugable ligand for cannabinoid CB 2 receptor imaging
    Mingfeng Bai
    Department of Chemistry, Vanderbilt University, VU Station B 351822, Nashville, Tennessee 37235 1822, USA
    Bioconjug Chem 19:988-92. 2008
    ..Preliminary evidence, including in vitro fluorescence imaging and a competition study, showed that NIRmbc94 specifically labeled CB 2-expressing cells...
  8. ncbi A novel conjugable translocator protein ligand labeled with a fluorescence dye for in vitro imaging
    Mingfeng Bai
    Department of Chemistry, Vanderbilt University, VU Station B 351822 Nashville, Tennessee 37235 1822, USA
    Bioconjug Chem 18:1118-22. 2007
    ..These findings suggest that C6Ro5-4864 may provide opportunities in imaging disease states where TSPO levels are affected, such as cancer and neurologic diseases...
  9. pmc Measurement of monovalent and polyvalent carbohydrate-lectin binding by back-scattering interferometry
    Amanda Kussrow
    Department of Chemistry and Vanderbilt Institute of Chemical Biology, Vanderbilt University, 4226 Stevenson Center, Nashville, Tennessee 37235, USA
    Anal Chem 81:4889-97. 2009
    ....
  10. ncbi The potential of backscattering interferometry as an in vitro clinical diagnostic tool for the serological diagnosis of infectious disease
    Amanda Kussrow
    Department of Chemistry and Vanderbilt Institute of Chemical Biology, Vanderbilt University, Nashville, Tennessee, USA
    Analyst 135:1535-7. 2010
    ....
  11. pmc TSPO targeted dendrimer imaging agent: synthesis, characterization, and cellular internalization
    Lynn E Samuelson
    Department of Chemistry, The Vanderbilt Institute for Chemical Biology and Vanderbilt Ingram Cancer Center, Vanderbilt University, VU Station B 351822 Nashville, Tennessee 37235 1822, USA
    Bioconjug Chem 20:2082-9. 2009
    ..Here, we present the synthesis, characterization, and cellular internalization into C6 rat glioma cells of a TSPO targeted dendrimer imaging agent...
  12. ncbi Molecular imaging of the translocator protein (TSPO) in a pre-clinical model of breast cancer
    Shelby K Wyatt
    Vanderbilt University Institute of Imaging Science, Vanderbilt University Medical Center, Nashville, TN, USA
    Mol Imaging Biol 12:349-58. 2010
    ..To quantitatively evaluate the utility of a translocator protein (TSPO)-targeted near-infrared (NIR) probe (NIR-conPK11195) for in vivo molecular imaging of TSPO in breast cancer...
  13. pmc Free-solution label-free detection of alpha-crystallin chaperone interactions by back-scattering interferometry
    Joey C Latham
    Department of Chemistry and The Vanderbilt Institute for Chemical Biology, Vanderbilt University, VU Station B 351822, Nashville, Tennessee 37235 1822, USA
    Anal Chem 81:1865-71. 2009
    ..This work demonstrates that BSI can be extended to screen libraries of disease-related mutants to quantify changes in affinity and/or kinetics of binding...
  14. ncbi A novel functional translocator protein ligand for cancer imaging
    Mingfeng Bai
    Department of Chemistry, Vanderbilt University, VU Station B 351822, Nashville, TN 37235 1822, USA
    Bioconjug Chem 18:2018-23. 2007
    ..Two fluorescent dye-labeled 6-TSPOmbb732 displayed nanomolar binding affinities to TSPO and were successfully imaged in vitro...
  15. ncbi Targeted imaging of colonic tumors in smad3-/- mice discriminates cancer and inflammation
    Natasha G Deane
    Department of Surgery and Division of Surgical Oncology, Vanderbilt University, Nashville, TN 37235, USA
    Mol Cancer Res 5:341-9. 2007
    ..Taken together, these results indicate that NIR-conPK11195 is a promising optical molecular imaging tool to rapidly screen for colonic tumors in mice and to discriminate inflammation from cancer...
  16. ncbi Noninvasive fluid flow measurements in microfluidic channels with backscatter interferometry
    Dmitry A Markov
    Department of Chemistry, Vanderbilt University, Nashville, TN 37235, USA
    Electrophoresis 25:3805-9. 2004
    ..The experiments are performed in the range of 3-6 microL/h with 3sigma detection limits determined to be 0.127 nL/s. Additionally, the RI response of the system is calibrated using temperature changes as well as glycerol solutions...
  17. ncbi Attomole sensitivity for unlabeled proteins and polypeptides with on-chip capillary electrophoresis and universal detection by interferometric backscatter
    Zhanling Wang
    Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX 79409 1061, USA
    Electrophoresis 24:865-73. 2003
    ..This development shows that a universal detector based on backscatter interferometry can be used effectively for on-chip label-free solute analysis...
  18. ncbi Facile, efficient conjugation of a trifunctional lanthanide chelate to a peripheral benzodiazepine receptor ligand
    H Charles Manning
    Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, Texas 79409, USA
    Org Lett 4:1075-8. 2002
    ..The contrast agent QM-CTMC-PK11195, when chelated with lanthanides, produces bright luminescence and good MRI contrast and can potentially serve as an imaging and demarcation agent for certain types of cancers...
  19. ncbi Quantification and evaluation of Joule heating in on-chip capillary electrophoresis
    Kelly Swinney
    Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX 79409 1061, USA
    Electrophoresis 23:613-20. 2002
    ..Results indicate that heating effects in on-chip CE have been underestimated and there is a need to readdress the theoretical model...

Research Grants1

  1. Interferometric Nano-sensing for Biochemical Analysis
    Darryl Bornhop; Fiscal Year: 2008
    ..This proposal expands on these results, culminating a multiplexed OCIBD for homogeneous (free-solution) molecular interaction assays. ..