SCOTT ENSIGN

Summary

Affiliation: Utah State University
Country: USA

Publications

  1. ncbi request reprint Revisiting the glyoxylate cycle: alternate pathways for microbial acetate assimilation
    Scott A Ensign
    Department of Chemistry and Biochemistry, Utah State University, Logan, UT 84322, USA
    Mol Microbiol 61:274-6. 2006
  2. ncbi request reprint New roles for CO2 in the microbial metabolism of aliphatic epoxides and ketones
    S A Ensign
    Department of Chemistry and Biochemistry, Utah State University, Logan, UT 84322 0300, USA
    Arch Microbiol 169:179-87. 1998
  3. ncbi request reprint Aliphatic epoxide carboxylation
    Scott A Ensign
    Department of Chemistry and Biochemistry, Utah State University, Logan, Utah 84322, USA
    Annu Rev Biochem 72:55-76. 2003
  4. ncbi request reprint Mechanistic implications of the structure of the mixed-disulfide intermediate of the disulfide oxidoreductase, 2-ketopropyl-coenzyme M oxidoreductase/carboxylase
    Arti S Pandey
    Department of Chemistry and Biochemistry, Montana State University, Bozeman, Montana 59717, USA
    Biochemistry 45:113-20. 2006
  5. pmc Characterization of 2-bromoethanesulfonate as a selective inhibitor of the coenzyme m-dependent pathway and enzymes of bacterial aliphatic epoxide metabolism
    Jeffrey M Boyd
    Department of Chemistry and Biochemistry, Utah State University, 0300 Old Main Hill, Logan, UT 84322, USA
    J Bacteriol 188:8062-9. 2006
  6. ncbi request reprint Structural basis for stereoselectivity in the (R)- and (S)-hydroxypropylthioethanesulfonate dehydrogenases
    Arathi M Krishnakumar
    Department of Chemistry and Biochemistry, Montana State University, Bozeman, Montana 59717, USA
    Biochemistry 45:8831-40. 2006
  7. pmc Mechanism of inhibition of aliphatic epoxide carboxylation by the coenzyme M analog 2-bromoethanesulfonate
    Jeffrey M Boyd
    Department of Chemistry and Biochemistry, Utah State University, Logan, Utah 84322 0300, USA
    J Biol Chem 285:25232-42. 2010
  8. ncbi request reprint The stereoselectivity and catalytic properties of Xanthobacter autotrophicus 2-[(R)-2-Hydroxypropylthio]ethanesulfonate dehydrogenase are controlled by interactions between C-terminal arginine residues and the sulfonate of coenzyme M
    Daniel D Clark
    Department of Chemistry and Biochemistry, Utah State University, Logan, Utah 84322 0300, USA
    Biochemistry 43:6763-71. 2004
  9. ncbi request reprint Bacterial acetone carboxylase is a manganese-dependent metalloenzyme
    Jeffrey M Boyd
    Department of Chemistry and Biochemistry, Utah State University, Logan, Utah 84322 0300, USA
    J Biol Chem 279:46644-51. 2004
  10. ncbi request reprint Structural basis for CO2 fixation by a novel member of the disulfide oxidoreductase family of enzymes, 2-ketopropyl-coenzyme M oxidoreductase/carboxylase
    Boguslaw Nocek
    Department of Chemistry and Biochemistry, Montana State University, Bozeman, Montana 59717, USA
    Biochemistry 41:12907-13. 2002

Research Grants

Collaborators

  • John Peters
  • Jeffrey M Boyd
  • Daniel D Clark
  • Boguslaw Nocek
  • Arathi M Krishnakumar
  • Jonathan G Krum
  • Arti S Pandey
  • Heather Ellsworth
  • Miriam K Sluis
  • Melissa A Kofoed
  • Darius Sliwa
  • Eric S Boyd
  • James A Endrizzi
  • Ashley Ellsworth
  • Boguslaw P Nocek
  • Jeffrey Boyd
  • Rachel A Larsen
  • Se Bok Jang
  • Gregory Rich
  • Ryan R Sargeant
  • Mi Suk Jeong
  • Ruth Anderson
  • William W Metcalf

Detail Information

Publications17

  1. ncbi request reprint Revisiting the glyoxylate cycle: alternate pathways for microbial acetate assimilation
    Scott A Ensign
    Department of Chemistry and Biochemistry, Utah State University, Logan, UT 84322, USA
    Mol Microbiol 61:274-6. 2006
    ..The details of these new pathways are the subject of this MicroCommentary...
  2. ncbi request reprint New roles for CO2 in the microbial metabolism of aliphatic epoxides and ketones
    S A Ensign
    Department of Chemistry and Biochemistry, Utah State University, Logan, UT 84322 0300, USA
    Arch Microbiol 169:179-87. 1998
    ..In addition, two new classes of carboxylases have been identified, the investigation of which promises to reveal new insights into biological strategies for the fixation of CO2 to organic substrates...
  3. ncbi request reprint Aliphatic epoxide carboxylation
    Scott A Ensign
    Department of Chemistry and Biochemistry, Utah State University, Logan, Utah 84322, USA
    Annu Rev Biochem 72:55-76. 2003
    ....
  4. ncbi request reprint Mechanistic implications of the structure of the mixed-disulfide intermediate of the disulfide oxidoreductase, 2-ketopropyl-coenzyme M oxidoreductase/carboxylase
    Arti S Pandey
    Department of Chemistry and Biochemistry, Montana State University, Bozeman, Montana 59717, USA
    Biochemistry 45:113-20. 2006
    ..The analysis of this structure and that of a coenzyme-M-bound form provides insights into the stabilization of intermediates, substrate carboxylation, and product release...
  5. pmc Characterization of 2-bromoethanesulfonate as a selective inhibitor of the coenzyme m-dependent pathway and enzymes of bacterial aliphatic epoxide metabolism
    Jeffrey M Boyd
    Department of Chemistry and Biochemistry, Utah State University, 0300 Old Main Hill, Logan, UT 84322, USA
    J Bacteriol 188:8062-9. 2006
    ..These results suggest that BES inhibits propylene-dependent growth and epoxide metabolism via irreversible inactivation of the key CO(2)-fixing enzyme 2-KPCC...
  6. ncbi request reprint Structural basis for stereoselectivity in the (R)- and (S)-hydroxypropylthioethanesulfonate dehydrogenases
    Arathi M Krishnakumar
    Department of Chemistry and Biochemistry, Montana State University, Bozeman, Montana 59717, USA
    Biochemistry 45:8831-40. 2006
    ....
  7. pmc Mechanism of inhibition of aliphatic epoxide carboxylation by the coenzyme M analog 2-bromoethanesulfonate
    Jeffrey M Boyd
    Department of Chemistry and Biochemistry, Utah State University, Logan, Utah 84322 0300, USA
    J Biol Chem 285:25232-42. 2010
    ..These results identify BES as a reactive CoM analog that specifically alkylates the interchange thiol that facilitates thioether bond cleavage and enolacetone formation during catalysis...
  8. ncbi request reprint The stereoselectivity and catalytic properties of Xanthobacter autotrophicus 2-[(R)-2-Hydroxypropylthio]ethanesulfonate dehydrogenase are controlled by interactions between C-terminal arginine residues and the sulfonate of coenzyme M
    Daniel D Clark
    Department of Chemistry and Biochemistry, Utah State University, Logan, Utah 84322 0300, USA
    Biochemistry 43:6763-71. 2004
    ..A model is proposed wherein sulfonate-arginine interactions within an alkylsulfonate binding pocket control the catalytic properties of R-HPCDH...
  9. ncbi request reprint Bacterial acetone carboxylase is a manganese-dependent metalloenzyme
    Jeffrey M Boyd
    Department of Chemistry and Biochemistry, Utah State University, Logan, Utah 84322 0300, USA
    J Biol Chem 279:46644-51. 2004
    ..These studies demonstrate that manganese is essential for acetone carboxylation and suggest a role for manganese in nucleotide binding and activation...
  10. ncbi request reprint Structural basis for CO2 fixation by a novel member of the disulfide oxidoreductase family of enzymes, 2-ketopropyl-coenzyme M oxidoreductase/carboxylase
    Boguslaw Nocek
    Department of Chemistry and Biochemistry, Montana State University, Bozeman, Montana 59717, USA
    Biochemistry 41:12907-13. 2002
    ..The encapsulation of the substrate in this manner is reminiscent of the conformational changes observed in the well-characterized CO2-fixing enzyme ribulose 1,5-bisphosphate carboxylase/oxidase (Rubisco)...
  11. ncbi request reprint Evidence for a metal-thiolate intermediate in alkyl group transfer from epoxypropane to coenzyme M and cooperative metal ion binding in epoxyalkane:CoM transferase
    Jeffrey M Boyd
    Department of Chemistry and Biochemistry, Utah State University, Logan, Utah 84322, USA
    Biochemistry 44:13151-62. 2005
    ..The divalent metal ion of EaCoMT is proposed to play a key role in the coordination and deprotonation of CoM, possibly through formation of a metal-thiolate that is activated for attack on the epoxide substrate...
  12. ncbi request reprint ATP-dependent enolization of acetone by acetone carboxylase from Rhodobacter capsulatus
    Jeffrey M Boyd
    Department of Chemistry and Biochemistry, Utah State University, Logan, Utah 84322, USA
    Biochemistry 44:8543-53. 2005
    ..It is proposed that alpha-proton abstraction from acetone occurs in concert with transfer of the gamma-phosphoryl group of ATP to the carbonyl oxygen, generating phosphoenol acetone as the activated nucleophile for attack on CO(2)...
  13. ncbi request reprint Crystallization and preliminary X-ray analysis of an acetone carboxylase from Xanthobacter autotrophicus strain Py2
    Boguslaw Nocek
    Department of Chemistry and Biochemistry, Montana State University, Bozeman MT 59717, USA
    Acta Crystallogr D Biol Crystallogr 60:385-7. 2004
    ..Crystals have been obtained that diffract to better than 2.8 A resolution and data have been collected to 3.2 A resolution...
  14. pmc Biochemical, molecular, and genetic analyses of the acetone carboxylases from Xanthobacter autotrophicus strain Py2 and Rhodobacter capsulatus strain B10
    Miriam K Sluis
    Department of Chemistry and Biochemistry, Utah State University, Logan, Utah 84322, USA
    J Bacteriol 184:2969-77. 2002
    ..capsulatus. These studies reveal a conserved strategy of ATP-dependent acetone carboxylation and the involvement of transcriptional enhancers in acetone carboxylase gene expression in gram-negative acetone-utilizing bacteria...
  15. ncbi request reprint Kinetic and microcalorimetric analysis of substrate and cofactor interactions in epoxyalkane:CoM transferase, a zinc-dependent epoxidase
    Jonathan G Krum
    Department of Chemistry and Biochemistry, Utah State University, Logan, Utah 84322 0300, USA
    Biochemistry 41:5005-14. 2002
    ....
  16. ncbi request reprint Crystallization and preliminary X-ray analysis of an R-2-hydroxypropyl-coenzyme M dehydrogenase
    Boguslaw Nocek
    Department of Chemistry and Biochemistry, Montana State University, Bozeman 59717, USA
    Acta Crystallogr D Biol Crystallogr 58:1470-3. 2002
    ..0 A resolution) could be achieved when the reaction substrates were added to the enzyme in a stoichiometric excess prior to crystallization...
  17. pmc Getting a handle on the role of coenzyme M in alkene metabolism
    Arathi M Krishnakumar
    Chemistry and Biochemistry Building, Montana State University, Bozeman, MT 59717, USA
    Microbiol Mol Biol Rev 72:445-56. 2008
    ..These studies provide the structural basis for understanding the efficacy of CoM as a handle to direct organic substrate transformations at the active sites of enzymes...

Research Grants9

  1. MICROBIAL METABOLISM OF ALIPHATIC ALKENES AND EPOXIDES
    SCOTT ENSIGN; Fiscal Year: 2004
    ..The epoxide-converting enzymes will be purified to homogeneity and characterized. ..
  2. Microbial metabolism of aliphatic alkenes & epoxides
    SCOTT ENSIGN; Fiscal Year: 2007
    ..We intend to further our work on the central roles of carboxylation and novel cofactors/enzymes in bacterial hydrocarbon/epoxide/ketone metabolism by pursuing the biochemical, structural, and genetic characterization of these processes. ..