R M Zucker

Summary

Affiliation: U.S. Environmental Protection Agency
Country: USA

Publications

  1. ncbi request reprint Evaluation of confocal microscopy system performance
    Robert M Zucker
    Reproductive Toxicology Division, National Health and Environmental Effects Research Laboratory, U S Environmental Protection Agency, Research Triangle Park, NC, USA
    Methods Mol Biol 319:77-135. 2006
  2. ncbi request reprint Confocal laser scanning microscopy of apoptosis in organogenesis-stage mouse embryos
    R M Zucker
    Reproductive Toxicology Division, National Health Effects and Environmental Research Laboratory, United States Environmental Protection Agency, Research Triangle Park, North Carolina 27711, USA
    Cytometry 33:348-54. 1998
  3. ncbi request reprint Practical confocal microscopy and the evaluation of system performance
    R M Zucker
    National Health and Environmental Effects Research Laboratory, U S Environmental Protection Agency, Research Triangle Park, North Carolina 27711, USA
    Methods 18:447-58. 1999
  4. ncbi request reprint Apoptosis and morphology in mouse embryos by confocal laser scanning microscopy
    R M Zucker
    National Health and Environmental Effects Research Laboratory, U S Environmental Protection Agency, Research Triangle Park, North Carolina 27711, USA
    Methods 18:473-80. 1999
  5. ncbi request reprint Whole insect and mammalian embryo imaging with confocal microscopy: morphology and apoptosis
    Robert M Zucker
    Division of Reproductive Toxicology, National Health and Environmental Effects Research Laboratory, Office of Research and Development, US Environmental Protection Agency, Research Triangle Park, North Carolina 27711, USA
    Cytometry A 69:1143-52. 2006
  6. ncbi request reprint Cytogenetic studies of three triazine herbicides. I. In vitro studies
    A D Kligerman
    Environmental Carcinogenesis Division, Genetic and Cellular Toxicology Branch, US Environmental Protection Agency, ERC Building, Mail Drop 68, Research Triangle Park, NC 27711, USA
    Mutat Res 465:53-9. 2000
  7. ncbi request reprint Evaluation of confocal microscopy system performance
    R M Zucker
    Reproductive Toxicology Division, National Health and Environmental Effects Research Laboratory, U S Environmental Protection Agency, Research Triangle Park, North Carolina 27711, USA
    Cytometry 44:273-94. 2001
  8. ncbi request reprint Nucleoside-mediated mitigation of 5-fluorouracil-induced toxicity in synchronized murine erythroleukemic cells
    K H Elstein
    National Health and Environmental Effects Research Laboratory, U S Environmental Protection Agency, Research Triangle Park, North Carolina 27711, USA
    Toxicol Appl Pharmacol 146:29-39. 1997
  9. doi request reprint Detection of TiO2 nanoparticles in cells by flow cytometry
    R M Zucker
    Toxicology Assessment Division MD 67, National Health and Environmental Effects Research Laboratory, Office of Research and Development, US Environmental Protection Agency, Research Triangle Park, North Carolina 27711, USA
    Cytometry A 77:677-85. 2010
  10. ncbi request reprint Statistical evaluation of confocal microscopy images
    R M Zucker
    Reproductive Toxicology Division, National Health and Environmental Effects Research Laboratory, U S Environmental Protection Agency, Research Triangle Park, North Carolina 27711, USA
    Cytometry 44:295-308. 2001

Detail Information

Publications26

  1. ncbi request reprint Evaluation of confocal microscopy system performance
    Robert M Zucker
    Reproductive Toxicology Division, National Health and Environmental Effects Research Laboratory, U S Environmental Protection Agency, Research Triangle Park, NC, USA
    Methods Mol Biol 319:77-135. 2006
    ..Utilization of this proposed testing approach will help eliminate the subjective nature of assessing the CLSM and allow different machines to be compared. These tests are essential if one is to make intensity measurements...
  2. ncbi request reprint Confocal laser scanning microscopy of apoptosis in organogenesis-stage mouse embryos
    R M Zucker
    Reproductive Toxicology Division, National Health Effects and Environmental Research Laboratory, United States Environmental Protection Agency, Research Triangle Park, North Carolina 27711, USA
    Cytometry 33:348-54. 1998
    ..This procedure allows for the optical imaging of whole day 9 (approximately 22 somites) embryos that were greater than 500 microns thick in the Z-axis...
  3. ncbi request reprint Practical confocal microscopy and the evaluation of system performance
    R M Zucker
    National Health and Environmental Effects Research Laboratory, U S Environmental Protection Agency, Research Triangle Park, North Carolina 27711, USA
    Methods 18:447-58. 1999
    ..Averaging can be used to improve the image at high PMT values, provided the sample is not bleached by repeated passes of the laser...
  4. ncbi request reprint Apoptosis and morphology in mouse embryos by confocal laser scanning microscopy
    R M Zucker
    National Health and Environmental Effects Research Laboratory, U S Environmental Protection Agency, Research Triangle Park, North Carolina 27711, USA
    Methods 18:473-80. 1999
    ....
  5. ncbi request reprint Whole insect and mammalian embryo imaging with confocal microscopy: morphology and apoptosis
    Robert M Zucker
    Division of Reproductive Toxicology, National Health and Environmental Effects Research Laboratory, Office of Research and Development, US Environmental Protection Agency, Research Triangle Park, North Carolina 27711, USA
    Cytometry A 69:1143-52. 2006
    ..LT has been shown to be an indicator of apoptotic cell death which is correlated to other standard apoptotic assays...
  6. ncbi request reprint Cytogenetic studies of three triazine herbicides. I. In vitro studies
    A D Kligerman
    Environmental Carcinogenesis Division, Genetic and Cellular Toxicology Branch, US Environmental Protection Agency, ERC Building, Mail Drop 68, Research Triangle Park, NC 27711, USA
    Mutat Res 465:53-9. 2000
    ..5% dimethyl sulfoxide (DMSO)]. Our results are discussed in light of contradictory results in the literature...
  7. ncbi request reprint Evaluation of confocal microscopy system performance
    R M Zucker
    Reproductive Toxicology Division, National Health and Environmental Effects Research Laboratory, U S Environmental Protection Agency, Research Triangle Park, North Carolina 27711, USA
    Cytometry 44:273-94. 2001
    ..The aim of this research was to develop quality assurance tests to ensure that the CLSM was stable while delivering reproducible intensity measurements with excellent image quality...
  8. ncbi request reprint Nucleoside-mediated mitigation of 5-fluorouracil-induced toxicity in synchronized murine erythroleukemic cells
    K H Elstein
    National Health and Environmental Effects Research Laboratory, U S Environmental Protection Agency, Research Triangle Park, North Carolina 27711, USA
    Toxicol Appl Pharmacol 146:29-39. 1997
    ....
  9. doi request reprint Detection of TiO2 nanoparticles in cells by flow cytometry
    R M Zucker
    Toxicology Assessment Division MD 67, National Health and Environmental Effects Research Laboratory, Office of Research and Development, US Environmental Protection Agency, Research Triangle Park, North Carolina 27711, USA
    Cytometry A 77:677-85. 2010
    ....
  10. ncbi request reprint Statistical evaluation of confocal microscopy images
    R M Zucker
    Reproductive Toxicology Division, National Health and Environmental Effects Research Laboratory, U S Environmental Protection Agency, Research Triangle Park, North Carolina 27711, USA
    Cytometry 44:295-308. 2001
    ..In a similar manner, the analysis of CV has been applied to the confocal laser scanning microscope (CLSM) to determine machine performance and sensitivity...
  11. ncbi request reprint Glutathione (GSH) concentrations vary with the cell cycle in maturing hamster oocytes, zygotes, and pre-implantation stage embryos
    Kurt A Zuelke
    USDA Agricultural Research Service, Animal and Natural Resources Institute, Germplasm and Gamete Physiology Laboratory, Bldg 200, Rm 124 BARC East, Beltsville, Maryland 20705, USA
    Mol Reprod Dev 64:106-12. 2003
    ..These observations raise the possibility that GSH synthesis in maturing oocytes is regulated by gonadotropins, and suggest that GSH is more important during fertilization than during pre-implantation embryo development...
  12. ncbi request reprint Perturbation of retinoic acid (RA)-mediated limb development suggests a role for diminished RA signaling in the teratogenesis of ethanol
    Corey S Johnson
    The Department of Cell and Developmental Biology, and Bowles Center for Alcohol Studies, The University of North Carolina, Chapel Hill, North Carolina, USA
    Birth Defects Res A Clin Mol Teratol 79:631-41. 2007
    ..A proposed mechanism for ethanol teratogenicity entails ethanol-mediated reductions in retinoic acid (RA). This premise was investigated utilizing a mouse model, with limb reduction defects as the teratogenic end point...
  13. ncbi request reprint Reliability of confocal microscopy spectral imaging systems: use of multispectral beads
    Robert M Zucker
    Reproductive Toxicology Division MD 67, National Health and Environmental Effects Research Laboratory, Office of Research and Development, U S Environmental Protection Agency, Research Triangle Park, North Carolina 27711, USA
    Cytometry A 71:174-89. 2007
    ..To achieve this goal, it is necessary to have testing tools to provide a reproducible way to evaluate instrument performance...
  14. ncbi request reprint Confocal laser scanning microscopy of whole mouse ovaries: excellent morphology, apoptosis detection, and spectroscopy
    Robert M Zucker
    National Health and Environmental Effects Research Laboratory, Reproductive Toxicology Division, Office of Research and Development, US Environmental Protection Agency, Research Triangle Park, NC, USA
    Cytometry A 69:930-9. 2006
    ..Many of these follicles will undergo an apoptotic process during the lifetime of the animal. By using proper tissue preparation methods, the events within the whole ovary can be observed by using 3D confocal microscopy...
  15. ncbi request reprint Quality assessment of confocal microscopy slide-based systems: instability
    Robert M Zucker
    Reproductive Toxicology Division, National Health and Environmental Effects Research Laboratory, Office of Research and Development, US Environmental Protection Agency, Research Triangle Park, NC 27711, USA
    Cytometry A 69:677-90. 2006
    ..Occasionally, this equipment becomes unstable, generating unreliable and inferior data...
  16. ncbi request reprint Quality assessment of confocal microscopy slide based systems: performance
    Robert M Zucker
    Reproductive Toxicology Division, National Health and Environmental Effects Research Laboratory, Office of Researchand Development US Environmental Protection Agency, Research Triangle Park, NC 27711, USA
    Cytometry A 69:659-76. 2006
    ..The optical principles employed is slide-based systems are similar to those of confocal microscopes (CLSM)...
  17. ncbi request reprint Wavelength and alignment tests for confocal spectral imaging systems
    Robert M Zucker
    U S Environmental Protection Agency Office of Research and Development, Reproductive Toxicology Division MD 67, National Health and Environmental Effects Research Laboratory, Research Triangle Park, North Carolina 27711, USA
    Microsc Res Tech 68:307-19. 2005
    ....
  18. ncbi request reprint Calibration and validation of confocal spectral imaging systems
    Jeremy M Lerner
    LightForm, Inc, 601 Route 206, Suite 26 479, Hillsborough, NJ 08844, USA
    Cytometry A 62:8-34. 2004
    ..This variability confirms that there is a need for a standardized, objective calibration and validation protocol...
  19. ncbi request reprint Developmental toxicity of methanol: Pathogenesis in CD-1 and C57BL/6J mice exposed in whole embryo culture
    Sigmund J Degitz
    United States Environmental Protection Agency, Research Triangle Park, North Carolina, USA
    Birth Defects Res A Clin Mol Teratol 70:179-84. 2004
    ..We evaluated methanol-induced pathogenesis in CD-1 and C57BL/6J embryos exposed during gastrulation in whole embryo culture...
  20. ncbi request reprint Pathogenesis of methanol-induced craniofacial defects in C57BL/6J mice
    Sigmund J Degitz
    United States Environmental Protection Agency, Research Triangle Park, North Carolina, USA
    Birth Defects Res A Clin Mol Teratol 70:172-8. 2004
    ..We describe dysmorphogenesis, cell death, cell cycle assessment, and effects on development of cranial ganglia and nerves observed following administration of methanol to pregnant C57BL/6J mice on gestation day (GD) 7...
  21. ncbi request reprint Localization of the sperm protein SP22 and inhibition of fertility in vivo and in vitro
    Gary R Klinefelter
    US Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Research Triangle Park, North Carolina 27711, USA
    J Androl 23:48-63. 2002
    ....
  22. ncbi request reprint Quantitative fluorescence of 5-FU-treated fetal rat limbs using confocal laser scanning microscopy and Lysotracker Red
    Owen T Price
    Reproductive Toxicology Division, National Health and Environmental Effects Research Laboratory, Office of Research Development, U S Environmental Protection Agency, Research Triangle Park, NC 27711, USA
    Cytometry A 53:9-21. 2003
    ..LT was shown previously to be an indicator of cell death in a manner similar to other standard assays (Annexin, terminal dUTP nick end labeling, Nile blue sulfate, neutral red, and acridine orange)...
  23. ncbi request reprint Abnormal fertilization is responsible for reduced fecundity following thiram-induced ovulatory delay in the rat
    Tammy E Stoker
    Reproductive Toxicology Division, Office of Research and Development, U S Environmental Protection Agency, Research Triangle Park, North Carolina 27711, USA
    Biol Reprod 68:2142-9. 2003
    ....
  24. ncbi request reprint Hindbrain and cranial nerve dysmorphogenesis result from acute maternal ethanol administration
    William C Dunty
    Department of Cell and Developmental Biology and the Bowles Center for Alcohol Studies, University of North Carolina at Chapel Hill, NC 27599 7090, USA
    Dev Neurosci 24:328-42. 2002
    ..These hindbrain and cranial nerve abnormalities are discussed in the context of the genesis of human alcohol-related birth defects and neurodevelopmental disorder...
  25. ncbi request reprint Methanol exposure during gastrulation causes holoprosencephaly, facial dysgenesis, and cervical vertebral malformations in C57BL/6J mice
    John M Rogers
    Reproductive Toxicology Division, National Health and Environmental Effects Research Laboratory, Office of Research and Development, United States Environmental Protection Agency, Research Triangle Park, North Carolina 27711, USA
    Birth Defects Res B Dev Reprod Toxicol 71:80-8. 2004
    ..C57BL/6J fetuses exhibit the holoprosencephaly spectrum of malformations after maternal exposure to ethanol during gastrulation, but the sensitivity of C57BL/6J mice to methanol-induced teratogenesis has not been previously described...
  26. ncbi request reprint A short history of the initial application of anti-5-BrdU to the detection and measurement of S phase
    Robert C Leif
    Newport Instruments, San Diego, California 92115 1022, USA
    Cytometry A 58:45-52. 2004