Research Topics
| R M ZuckerSummaryAffiliation: U.S. Environmental Protection Agency Country: USA Publications
| Collaborators
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Detail Information
Publications
Detection of TiO2 nanoparticles in cells by flow cytometryRobert Martin Zucker
Toxicology Assessment Division, Office of Research and Development, National Health and Environmental Effects Research Laboratory, US Environmental Protection Agency, Research Triangle Park, NC, USA
Methods Mol Biol 906:497-509. 2012..Such experiments could potentially be performed quickly and easily using the flow cytometer to measure both nanoparticle uptake and cellular health...- Microscopy imaging methods for the detection of silver and titanium nanoparticles within cellsRobert Martin Zucker
Toxicology Assessment Division, Office of Research and Development, National Health and Environmental Effects Research Laboratory, US Environmental Protection Agency, Research Triangle Park, NC, USA
Methods Mol Biol 906:483-96. 2012..This report details how wide-field microscopy can be effectively used to detect nanoparticle uptake as well as to assess cellular health in ARPE-19 cell cultures... 
Darkfield-confocal microscopy detection of nanoscale particle internalization by human lung cellsEugene A Gibbs-Flournoy
Environmental Public Health Division, National Health and Environmental Effects Research Laboratory, U S Environmental Protection Agency, Chapel Hill, NC, USA
Part Fibre Toxicol 8:2. 2011....
Quality assessment of confocal microscopy slide-based systems: instabilityRobert M Zucker
Reproductive Toxicology Division, National Health and Environmental Effects Research Laboratory, Office of Research and Development, US Environmental Protection Agency, Research Triangle Park, NC 27711, USA
Cytometry A 69:677-90. 2006..Occasionally, this equipment becomes unstable, generating unreliable and inferior data...- Confocal laser scanning microscopy of whole mouse ovaries: excellent morphology, apoptosis detection, and spectroscopyRobert M Zucker
National Health and Environmental Effects Research Laboratory, Reproductive Toxicology Division, Office of Research and Development, US Environmental Protection Agency, Research Triangle Park, NC, USA
Cytometry A 69:930-9. 2006..Many of these follicles will undergo an apoptotic process during the lifetime of the animal. By using proper tissue preparation methods, the events within the whole ovary can be observed by using 3D confocal microscopy... - Statistical evaluation of confocal microscopy imagesR M Zucker
Reproductive Toxicology Division, National Health and Environmental Effects Research Laboratory, U S Environmental Protection Agency, Research Triangle Park, North Carolina 27711, USA
Cytometry 44:295-308. 2001..In a similar manner, the analysis of CV has been applied to the confocal laser scanning microscope (CLSM) to determine machine performance and sensitivity... - Evaluation of confocal microscopy system performanceR M Zucker
Reproductive Toxicology Division, National Health and Environmental Effects Research Laboratory, U S Environmental Protection Agency, Research Triangle Park, North Carolina 27711, USA
Cytometry 44:273-94. 2001..The aim of this research was to develop quality assurance tests to ensure that the CLSM was stable while delivering reproducible intensity measurements with excellent image quality... - Cytogenetic studies of three triazine herbicides. I. In vitro studiesA D Kligerman
Environmental Carcinogenesis Division, Genetic and Cellular Toxicology Branch, US Environmental Protection Agency, ERC Building, Mail Drop 68, Research Triangle Park, NC 27711, USA
Mutat Res 465:53-9. 2000..5% dimethyl sulfoxide (DMSO)]. Our results are discussed in light of contradictory results in the literature... - Detection of TiO2 nanoparticles in cells by flow cytometryR M Zucker
Toxicology Assessment Division MD 67, National Health and Environmental Effects Research Laboratory, Office of Research and Development, US Environmental Protection Agency, Research Triangle Park, North Carolina 27711, USA
Cytometry A 77:677-85. 2010.... - Nucleoside-mediated mitigation of 5-fluorouracil-induced toxicity in synchronized murine erythroleukemic cellsK H Elstein
National Health and Environmental Effects Research Laboratory, U S Environmental Protection Agency, Research Triangle Park, North Carolina 27711, USA
Toxicol Appl Pharmacol 146:29-39. 1997.... - Confocal laser scanning microscopy of apoptosis in organogenesis-stage mouse embryosR M Zucker
Reproductive Toxicology Division, National Health Effects and Environmental Research Laboratory, United States Environmental Protection Agency, Research Triangle Park, North Carolina 27711, USA
Cytometry 33:348-54. 1998..This procedure allows for the optical imaging of whole day 9 (approximately 22 somites) embryos that were greater than 500 microns thick in the Z-axis... - Practical confocal microscopy and the evaluation of system performanceR M Zucker
National Health and Environmental Effects Research Laboratory, U S Environmental Protection Agency, Research Triangle Park, North Carolina 27711, USA
Methods 18:447-58. 1999..Averaging can be used to improve the image at high PMT values, provided the sample is not bleached by repeated passes of the laser... - Apoptosis and morphology in mouse embryos by confocal laser scanning microscopyR M Zucker
National Health and Environmental Effects Research Laboratory, U S Environmental Protection Agency, Research Triangle Park, North Carolina 27711, USA
Methods 18:473-80. 1999.... - Abnormal fertilization is responsible for reduced fecundity following thiram-induced ovulatory delay in the ratTammy E Stoker
Reproductive Toxicology Division, Office of Research and Development, U S Environmental Protection Agency, Research Triangle Park, North Carolina 27711, USA
Biol Reprod 68:2142-9. 2003.... - Perturbation of retinoic acid (RA)-mediated limb development suggests a role for diminished RA signaling in the teratogenesis of ethanolCorey S Johnson
The Department of Cell and Developmental Biology, and Bowles Center for Alcohol Studies, The University of North Carolina, Chapel Hill, North Carolina, USA
Birth Defects Res A Clin Mol Teratol 79:631-41. 2007..A proposed mechanism for ethanol teratogenicity entails ethanol-mediated reductions in retinoic acid (RA). This premise was investigated utilizing a mouse model, with limb reduction defects as the teratogenic end point... - Reliability of confocal microscopy spectral imaging systems: use of multispectral beadsRobert M Zucker
Reproductive Toxicology Division MD 67, National Health and Environmental Effects Research Laboratory, Office of Research and Development, U S Environmental Protection Agency, Research Triangle Park, North Carolina 27711, USA
Cytometry A 71:174-89. 2007..To achieve this goal, it is necessary to have testing tools to provide a reproducible way to evaluate instrument performance... - Whole insect and mammalian embryo imaging with confocal microscopy: morphology and apoptosisRobert M Zucker
Division of Reproductive Toxicology, National Health and Environmental Effects Research Laboratory, Office of Research and Development, US Environmental Protection Agency, Research Triangle Park, North Carolina 27711, USA
Cytometry A 69:1143-52. 2006..LT has been shown to be an indicator of apoptotic cell death which is correlated to other standard apoptotic assays... - Localization of the sperm protein SP22 and inhibition of fertility in vivo and in vitroGary R Klinefelter
US Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Research Triangle Park, North Carolina 27711, USA
J Androl 23:48-63. 2002.... - Glutathione (GSH) concentrations vary with the cell cycle in maturing hamster oocytes, zygotes, and pre-implantation stage embryosKurt A Zuelke
USDA Agricultural Research Service, Animal and Natural Resources Institute, Germplasm and Gamete Physiology Laboratory, Bldg 200, Rm 124 BARC-East, Beltsville, Maryland 20705, USA
Mol Reprod Dev 64:106-12. 2003..These observations raise the possibility that GSH synthesis in maturing oocytes is regulated by gonadotropins, and suggest that GSH is more important during fertilization than during pre-implantation embryo development... - Quality assessment of confocal microscopy slide based systems: performanceRobert M Zucker
Reproductive Toxicology Division, National Health and Environmental Effects Research Laboratory, Office of Researchand Development US Environmental Protection Agency, Research Triangle Park, NC 27711, USA
Cytometry A 69:659-76. 2006..The optical principles employed is slide-based systems are similar to those of confocal microscopes (CLSM)... - Evaluation of confocal microscopy system performanceRobert M Zucker
Reproductive Toxicology Division, National Health and Environmental Effects Research Laboratory, U.S. Environmental Protection Agency, Research Triangle Park, NC, USA
Methods Mol Biol 319:77-135. 2006..Utilization of this proposed testing approach will help eliminate the subjective nature of assessing the CLSM and allow different machines to be compared. These tests are essential if one is to make intensity measurements... - Wavelength and alignment tests for confocal spectral imaging systemsRobert M Zucker
U S Environmental Protection Agency Office of Research and Development, Reproductive Toxicology Division MD 67, National Health and Environmental Effects Research Laboratory, Research Triangle Park, North Carolina 27711, USA
Microsc Res Tech 68:307-19. 2005.... - Calibration and validation of confocal spectral imaging systemsJeremy M Lerner
LightForm, Inc, 601 Route 206, Suite 26 479, Hillsborough, NJ 08844, USA
Cytometry A 62:8-34. 2004..This variability confirms that there is a need for a standardized, objective calibration and validation protocol... - Developmental toxicity of methanol: Pathogenesis in CD-1 and C57BL/6J mice exposed in whole embryo cultureSigmund J Degitz
United States Environmental Protection Agency, Research Triangle Park, North Carolina, USA
Birth Defects Res A Clin Mol Teratol 70:179-84. 2004..We evaluated methanol-induced pathogenesis in CD-1 and C57BL/6J embryos exposed during gastrulation in whole embryo culture... - Hindbrain and cranial nerve dysmorphogenesis result from acute maternal ethanol administrationWilliam C Dunty
Department of Cell and Developmental Biology and the Bowles Center for Alcohol Studies, University of North Carolina at Chapel Hill, NC 27599-7090, USA
Dev Neurosci 24:328-42. 2002..These hindbrain and cranial nerve abnormalities are discussed in the context of the genesis of human alcohol-related birth defects and neurodevelopmental disorder... - Methanol exposure during gastrulation causes holoprosencephaly, facial dysgenesis, and cervical vertebral malformations in C57BL/6J miceJohn M Rogers
Reproductive Toxicology Division, National Health and Environmental Effects Research Laboratory, Office of Research and Development, United States Environmental Protection Agency, Research Triangle Park, North Carolina 27711, USA
Birth Defects Res B Dev Reprod Toxicol 71:80-8. 2004..C57BL/6J fetuses exhibit the holoprosencephaly spectrum of malformations after maternal exposure to ethanol during gastrulation, but the sensitivity of C57BL/6J mice to methanol-induced teratogenesis has not been previously described... - Quantitative fluorescence of 5-FU-treated fetal rat limbs using confocal laser scanning microscopy and Lysotracker RedOwen T Price
Reproductive Toxicology Division, National Health and Environmental Effects Research Laboratory, Office of Research Development, U.S. Environmental Protection Agency, Research Triangle Park, NC 27711, USA
Cytometry A 53:9-21. 2003.... - Pathogenesis of methanol-induced craniofacial defects in C57BL/6J miceSigmund J Degitz
United States Environmental Protection Agency, Research Triangle Park, North Carolina, USA
Birth Defects Res A Clin Mol Teratol 70:172-8. 2004..We describe dysmorphogenesis, cell death, cell cycle assessment, and effects on development of cranial ganglia and nerves observed following administration of methanol to pregnant C57BL/6J mice on gestation day (GD) 7... - A short history of the initial application of anti-5-BrdU to the detection and measurement of S phaseRobert C Leif
Newport Instruments, San Diego, California 92115-1022, USA
Cytometry A 58:45-52. 2004