Steven O Simmons
Affiliation: U.S. Environmental Protection Agency
Paul Friedman K, Watt E, Hornung M, Hedge J, Judson R, Crofton K, et al
. Tiered High-Throughput Screening Approach to Identify Thyroperoxidase Inhibitors Within the ToxCast Phase I and II Chemical Libraries. Toxicol Sci. 2016;151:160-80 pubmed publisher
..This effort represents the most extensive TPO inhibition screening campaign to date and illustrates a tiered screening approach that focuses resources, maximizes assay throughput, and reduces animal use. ..
Simmons S. Fireflies in the coalmine: luciferase technologies in next-generation toxicity testing. Comb Chem High Throughput Screen. 2011;14:688-702 pubmed
DeGroot D, Swank A, Thomas R, Strynar M, Lee M, Carmichael P, et al
. mRNA transfection retrofits cell-based assays with xenobiotic metabolism. J Pharmacol Toxicol Methods. 2018;92:77-94 pubmed publisher
..Overall, transfection of CYP-encoding mRNAs is an effective and portable solution for retrofitting existing cell-based HTS assays with metabolic competence. ..
Lavrich K, Corteselli E, Wages P, Bromberg P, Simmons S, Gibbs Flournoy E, et al
. Investigating mitochondrial dysfunction in human lung cells exposed to redox-active PM components. Toxicol Appl Pharmacol. 2018;342:99-107 pubmed publisher
..This study also highlights the methodological utility and challenges in the use of extracellular flux analysis to elucidate the mechanisms of action of redox-active electrophiles present in ambient air. ..