Stephanie Padilla

Summary

Affiliation: U.S. Environmental Protection Agency
Country: USA

Publications

  1. doi request reprint Zebrafish developmental screening of the ToxCastâ„¢ Phase I chemical library
    S Padilla
    National Health and Environmental Effects Research Laboratory, Office of Research and Development, US Environmental Protection Agency, Research Triangle Park, NC 27712, USA
    Reprod Toxicol 33:174-87. 2012
  2. ncbi request reprint Time course of cholinesterase inhibition in adult rats treated acutely with carbaryl, carbofuran, formetanate, methomyl, methiocarb, oxamyl or propoxur
    S Padilla
    Cellular and Molecular Toxicology Branch, Neurotoxicology Division, U S Environmental Protection Agency, Research Triangle Park, NC 27711, USA
    Toxicol Appl Pharmacol 219:202-9. 2007
  3. ncbi request reprint Neurochemical effects of chronic dietary and repeated high-level acute exposure to chlorpyrifos in rats
    Stephanie Padilla
    Neurotoxicology Division, US Environmental Protection Agency, Research Triangle Park, North Carolina 27711, USA
    Toxicol Sci 88:161-71. 2005
  4. ncbi request reprint Further assessment of an in vitro screen that may help identify organophosphorus pesticides that are more acutely toxic to the young
    S Padilla
    Neurotoxicology Division, National Health and Environmental Effects Research Laboratory, Office of Research and Development, U S Environmental Protection Agency, North Carolina 27711, USA
    J Toxicol Environ Health A 67:1477-89. 2004
  5. ncbi request reprint Comparison of the role of esterases in the differential age-related sensitivity to chlorpyrifos and methamidophos
    S Padilla
    Neurotoxicology Division, U S Environmental Protection Agency, Research Triangle Park, North Carolina 27711, USA
    Neurotoxicology 21:49-56. 2000
  6. doi request reprint Assessing locomotor activity in larval zebrafish: Influence of extrinsic and intrinsic variables
    S Padilla
    Integrated Systems Toxicology Division, National Health and Environmental Effects Research Laboratory, Office of Research and Development, U S Environmental Protection Agency, Research Triangle Park, NC 27711, United States
    Neurotoxicol Teratol 33:624-30. 2011
  7. doi request reprint Generation and characterization of neurogenin1-GFP transgenic medaka with potential for rapid developmental neurotoxicity screening
    Chun Yang Fan
    Integrated Systems Toxicology and Toxicity Assessment Divisions, National Health and Environmental Effects Research Laboratory, US EPA, Research Triangle Park, NC 27711, USA
    Aquat Toxicol 105:127-35. 2011
  8. doi request reprint Developmental exposure to valproate and ethanol alters locomotor activity and retino-tectal projection area in zebrafish embryos
    John Cowden
    Integrated Systems Toxicology Division United States Environmental Protection Agency, Research Triangle Park, NC 27711, USA
    Reprod Toxicol 33:165-73. 2012
  9. ncbi request reprint Comparison of acute neurobehavioral and cholinesterase inhibitory effects of N-methylcarbamates in rat
    Katherine L McDaniel
    Neurotoxicology Division, National Health and Environmental Effects Research Laboratory, Office of Research and Development, US Environmental Protection Agency, Research Triangle Park, North Carolina 27711, USA
    Toxicol Sci 98:552-60. 2007
  10. doi request reprint Impact of chemical proportions on the acute neurotoxicity of a mixture of seven carbamates in preweanling and adult rats
    Virginia C Moser
    Toxicity Assessment Division, US EPA, Research Triangle Park, North Carolina 27711, USA
    Toxicol Sci 129:126-34. 2012

Collaborators

Detail Information

Publications33

  1. doi request reprint Zebrafish developmental screening of the ToxCastâ„¢ Phase I chemical library
    S Padilla
    National Health and Environmental Effects Research Laboratory, Office of Research and Development, US Environmental Protection Agency, Research Triangle Park, NC 27712, USA
    Reprod Toxicol 33:174-87. 2012
    ..The zebrafish embryo screen, by providing an integrated model of the developing vertebrate, compliments the ToxCast assay portfolio and has the potential to provide information relative to overt and organismal toxicity...
  2. ncbi request reprint Time course of cholinesterase inhibition in adult rats treated acutely with carbaryl, carbofuran, formetanate, methomyl, methiocarb, oxamyl or propoxur
    S Padilla
    Cellular and Molecular Toxicology Branch, Neurotoxicology Division, U S Environmental Protection Agency, Research Triangle Park, NC 27711, USA
    Toxicol Appl Pharmacol 219:202-9. 2007
    ....
  3. ncbi request reprint Neurochemical effects of chronic dietary and repeated high-level acute exposure to chlorpyrifos in rats
    Stephanie Padilla
    Neurotoxicology Division, US Environmental Protection Agency, Research Triangle Park, North Carolina 27711, USA
    Toxicol Sci 88:161-71. 2005
    ..These data indicate that, although chronic feeding with or without intermittent spiked dosages with CPF produces substantial biochemical changes in a dose- and tissue-related manner, there are no persistent biochemical changes...
  4. ncbi request reprint Further assessment of an in vitro screen that may help identify organophosphorus pesticides that are more acutely toxic to the young
    S Padilla
    Neurotoxicology Division, National Health and Environmental Effects Research Laboratory, Office of Research and Development, U S Environmental Protection Agency, North Carolina 27711, USA
    J Toxicol Environ Health A 67:1477-89. 2004
    ..We conclude that our in vitro screen may prove to be a useful, quick, convenient test for identifying which organophosphorus pesticides may be more acutely toxic to the young as compared to adults...
  5. ncbi request reprint Comparison of the role of esterases in the differential age-related sensitivity to chlorpyrifos and methamidophos
    S Padilla
    Neurotoxicology Division, U S Environmental Protection Agency, Research Triangle Park, North Carolina 27711, USA
    Neurotoxicology 21:49-56. 2000
    ....
  6. doi request reprint Assessing locomotor activity in larval zebrafish: Influence of extrinsic and intrinsic variables
    S Padilla
    Integrated Systems Toxicology Division, National Health and Environmental Effects Research Laboratory, Office of Research and Development, U S Environmental Protection Agency, Research Triangle Park, NC 27711, United States
    Neurotoxicol Teratol 33:624-30. 2011
    ..Zebrafish larval behavior using a microtiter plate format may be an ideal endpoint for screening developmentally neurotoxic chemicals, but it is imperative that many test variables be carefully specified and controlled...
  7. doi request reprint Generation and characterization of neurogenin1-GFP transgenic medaka with potential for rapid developmental neurotoxicity screening
    Chun Yang Fan
    Integrated Systems Toxicology and Toxicity Assessment Divisions, National Health and Environmental Effects Research Laboratory, US EPA, Research Triangle Park, NC 27711, USA
    Aquat Toxicol 105:127-35. 2011
    ..This strain has been deposited with and maintained by the National BioResource Project and is available on request (http://www.shigen.nig.ac.jp/medaka/strainDetailAction.do?quickSearch=true&strainId=5660)...
  8. doi request reprint Developmental exposure to valproate and ethanol alters locomotor activity and retino-tectal projection area in zebrafish embryos
    John Cowden
    Integrated Systems Toxicology Division United States Environmental Protection Agency, Research Triangle Park, NC 27711, USA
    Reprod Toxicol 33:165-73. 2012
    ..As both valproate and ethanol are known human developmental neurotoxicants, these results add to the growing body of evidence showing the potential utility of zebrafish in screening compounds for mammalian developmental neurotoxicity...
  9. ncbi request reprint Comparison of acute neurobehavioral and cholinesterase inhibitory effects of N-methylcarbamates in rat
    Katherine L McDaniel
    Neurotoxicology Division, National Health and Environmental Effects Research Laboratory, Office of Research and Development, US Environmental Protection Agency, Research Triangle Park, North Carolina 27711, USA
    Toxicol Sci 98:552-60. 2007
    ..Thus, motor activity decreases are highly predictive of ChE inhibition for N-methyl carbamates, and vice versa...
  10. doi request reprint Impact of chemical proportions on the acute neurotoxicity of a mixture of seven carbamates in preweanling and adult rats
    Virginia C Moser
    Toxicity Assessment Division, US EPA, Research Triangle Park, North Carolina 27711, USA
    Toxicol Sci 129:126-34. 2012
    ..These approaches for studying pesticide mixtures can improve evaluations of potential toxicity under varying experimental conditions that may mimic human exposures...
  11. doi request reprint Use of medaka in toxicity testing
    Stephanie Padilla
    United States Environmental Protection Agency, Research Triangle Park, North Carolina, USA
    Curr Protoc Toxicol . 2009
    ..The methods run the gamut from immunohistology through PCR to basic histological techniques...
  12. doi request reprint Relationship between brain and plasma carbaryl levels and cholinesterase inhibition
    David W Herr
    Neurotoxicology Branch, Toxicity Assessment Division, National Health and Environmental Effects Research Laboratory, Office of Research and Development, U S EPA, Research Triangle Park, NC 27711, United States
    Toxicology 276:172-83. 2010
    ..The results of these experiments will be useful to extend physiologically-based pharmacokinetic models for carbaryl and their application in risk assessment...
  13. ncbi request reprint Adapting the medaka embryo assay to a high-throughput approach for developmental toxicity testing
    Sharon L Oxendine
    Curriculum in Toxicology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Neurotoxicology 27:840-5. 2006
    ....
  14. ncbi request reprint Vulnerable windows for developmental ethanol toxicity in the Japanese medaka fish (Oryzias latipes)
    Sharon L Oxendine
    Curriculum in Toxicology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, United States
    Aquat Toxicol 80:396-404. 2006
    ..In general, these data suggest that critical periods for heightened sensitivity to developmental EtOH exposure may vary according to the specific endpoint used to assess toxicity...
  15. doi request reprint Esterase metabolism of cholinesterase inhibitors using rat liver in vitro
    V C Moser
    Toxicity Assessment Division MD B105 04, National Health and Environmental Effects Research Laboratory, Office of Research and Development, US Environmental Protection Agency, Research Triangle Park, NC 27711, USA
    Toxicology 281:56-62. 2011
    ..Thus, generalizations regarding these metabolic pathways may not be appropriate. As with other aspects of AChE inhibitors, their metabolic patterns appear to be chemical-specific...
  16. ncbi request reprint Gestational exposure to chlorpyrifos: comparative distribution of trichloropyridinol in the fetus and dam
    D L Hunter
    Neurotoxicology Division, U S Environmental Protection Agency, Research Triangle Park, North Carolina 27711, USA
    Toxicol Appl Pharmacol 158:16-23. 1999
    ..These toxicokinetic results suggest that the fetal nervous system may be exposed to a higher concentration of chlorpyrifos than the maternal nervous system when the dam is orally exposed to chlorpyrifos during late gestation...
  17. doi request reprint Locomotion in larval zebrafish: Influence of time of day, lighting and ethanol
    R C MacPhail
    Neurotoxicology Division, National Health and Environmental Effects Research Laboratory, U S Environmental Protection Agency, Research Triangle Park, NC 27711, USA
    Neurotoxicology 30:52-8. 2009
    ..Collectively, these results show that locomotion in larval zebrafish can be reliably measured in a 96-well microtiter plate format, and is sensitive to time of day, lighting conditions, and ethanol...
  18. ncbi request reprint Gestational exposure to chlorpyrifos: dose response profiles for cholinesterase and carboxylesterase activity
    T L Lassiter
    Curriculum in Toxicology, University of North Carolina, Chapel Hill, USA
    Toxicol Sci 52:92-100. 1999
    ..Both the in vivo and in vitro findings indicated that fetal liver CaE inhibition was an extremely sensitive indicator of fetal chlorpyrifos exposure...
  19. doi request reprint Zebrafish: as an integrative model for twenty-first century toxicity testing
    Nisha S Sipes
    National Center for Computational Toxicology, U S Environmental Protection Agency, Research Triangle Park, North Carolina, USA
    Birth Defects Res C Embryo Today 93:256-67. 2011
    ..Cross-species comparisons implied a common basis for biological pathways associated with neuronal defects, extracellular matrix remodeling, and mitotic arrest...
  20. ncbi request reprint Measuring cholinesterase activity in human saliva
    Birgit Claus Henn
    U S Environmental Protection Agency, Neurotoxicology Division and Human Studies Division, National Health and Environmental Effects Research Laboratory, Office of Research and Development, Research Triangle Park, North Carolina 27711, USA
    J Toxicol Environ Health A 69:1805-18. 2006
    ..Results from this study suggest that saliva may be a useful indicator of potential neurotoxic effects from exposure to organophosphorus and carbamate pesticides, but that factors affecting variability should be explored further...
  21. ncbi request reprint A dried blood spot method to evaluate cholinesterase activity in young children
    Elizabeth D Hilborn
    National Health and Environmental Effects Research Laboratory, Office of Research and Development, US Environmental Protection Agency, Research Triangle Park, North Carolina 27711, USA
    Arch Environ Health 59:467-70. 2004
    ..6 (p = 0.01). This dried blood method may be used during field studies to evaluate changes in cholinesterase values in children over time...
  22. ncbi request reprint Neurobehavioral effects of chronic dietary and repeated high-level spike exposure to chlorpyrifos in rats
    V C Moser
    Neurotoxicology Division, National Health and Environmental Effects Research Laboratory, Office of Research and Development, US Environmental Protection Agency, Research Triangle Park, North Carolina 27711, USA
    Toxicol Sci 86:375-86. 2005
    ..Overall, dietary exposure to chlorpyrifos produced long-lasting neurobehavioral changes and also altered the response to acute challenges...
  23. doi request reprint Update on EPA's ToxCast program: providing high throughput decision support tools for chemical risk management
    Robert Kavlock
    National Center for Computational Toxicology, Office of Research and Development, U S Environmental Protection Agency, Research Triangle Park, NC 27711, USA
    Chem Res Toxicol 25:1287-302. 2012
    ..This perspective provides a summary of the initial, proof of concept, Phase I of ToxCast that has laid the groundwork for the next phases and future directions of the program...
  24. doi request reprint Gene expression changes in developing zebrafish as potential markers for rapid developmental neurotoxicity screening
    Chun Yang Fan
    Cellular and Molecular Toxicology Branch, Neurotoxicology Division, United States Environmental Protection Agency, Research Triangle Park, NC 27711, USA
    Neurotoxicol Teratol 32:91-8. 2010
    ..These results suggest that the expression profiles of these genes may be useful biomarkers for rapid evaluation of the developmental neurotoxicity potential of chemicals...
  25. ncbi request reprint Murine susceptibility to organophosphorus-induced delayed neuropathy (OPIDN)
    B Veronesi
    U S Environmental Protection Agency, Health Effects Research Laboratory, Research Triangle Park, North Carolina 27711
    Toxicol Appl Pharmacol 107:311-24. 1991
    ....
  26. ncbi request reprint Paraoxon toxicity is not potentiated by prior reduction in blood acetylcholinesterase
    S Padilla
    Neurotoxicology Division MD 74B, U S Environmental Protection Agency, Research Triangle Park, North Carolina 27711
    Toxicol Appl Pharmacol 117:110-5. 1992
    ..Thus, significant loss in blood AChE alone produced no detectable neurobehavioral deficits and did not alter the subsequent responses to paraoxon challenge...
  27. pmc Methods to identify and characterize developmental neurotoxicity for human health risk assessment. III: pharmacokinetic and pharmacodynamic considerations
    D C Dorman
    Chemical Industry Institute of Toxicology, Research Triangle Park, North Carolina, USA
    Environ Health Perspect 109:101-11. 2001
    ....
  28. ncbi request reprint Re: age-related brain cholinesterase inhibition kinetics following in vitro incubation with chlorpyrifos-oxon and diazinon-oxon
    Stephanie Padilla
    Toxicol Sci 98:604; author reply 605-6. 2007
  29. pmc Developmental neurotoxicity of chlorpyrifos: what is the vulnerable period?
    Dan Qiao
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    Environ Health Perspect 110:1097-103. 2002
    ..Although delayed neurotoxic effects of prenatal CPF may emerge subsequently in development, our results are consistent with the preferential targeting of late developmental events such as gliogenesis, axonogenesis, and synaptogenesis...
  30. ncbi request reprint Ontogeny of hepatic and plasma metabolism of deltamethrin in vitro: role in age-dependent acute neurotoxicity
    Sathanandam S Anand
    Department of Pharmaceutical and Biomedical Sciences, College of Pharmacy, University of Georgia, Athens, GA 30602, USA
    Drug Metab Dispos 34:389-97. 2006
    ..The present study provides evidence that the limited metabolic capacity of immature rats contributes to elevated systemic exposure and ensuing neurotoxic effects of DLM...
  31. ncbi request reprint Characterization of deltamethrin metabolism by rat plasma and liver microsomes
    Sathanandam S Anand
    Department of Pharmaceutical and Biomedical Sciences, College of Pharmacy, University of Georgia, Athens, GA 30602, USA
    Toxicol Appl Pharmacol 212:156-66. 2006
    ..CYP1A2, CYP1A1, and CYP2C11 in decreasing order of importance quantitatively, metabolized deltamethrin. Intrinsic clearance by liver CYP450s (35.5) was more efficient than that by liver (12.0) or plasma carboxylesterases (2.4)...
  32. ncbi request reprint A tiered approach to systemic toxicity testing for agricultural chemical safety assessment
    John E Doe
    Syngenta CTL, Macclesfield, United Kingdom
    Crit Rev Toxicol 36:37-68. 2006
    ....
  33. ncbi request reprint Automated measurement of acetylcholinesterase activity in rat peripheral tissues
    T Leon Lassiter
    Curriculum in Toxicology, University of North Carolina, Chapel Hill, NC 27516, USA
    Toxicology 186:241-53. 2003
    ..In conclusion, automated assay of acetylcholinesterase activity in cholinergically innervated tissues in the rat (other than brain) is achievable and relatively convenient...