L E Gray

Summary

Affiliation: U.S. Environmental Protection Agency
Country: USA

Publications

  1. ncbi request reprint Administration of potentially antiandrogenic pesticides (procymidone, linuron, iprodione, chlozolinate, p,p'-DDE, and ketoconazole) and toxic substances (dibutyl- and diethylhexyl phthalate, PCB 169, and ethane dimethane sulphonate) during sexual differen
    C Wolf
    Endocrinology Branch, National Health and Environmental Effects Research Laboratory, US Environmental Protection Agency, MD 72, Research Triangle Park, NC 27711, USA
    Toxicol Ind Health 15:94-118. 1999
  2. ncbi request reprint The fungicide procymidone alters sexual differentiation in the male rat by acting as an androgen-receptor antagonist in vivo and in vitro
    J Ostby
    Endocrinology Branch, National Health and Environmental Effects Research Laboratory, US EPA, Research Triangle Park, NC 27711, USA
    Toxicol Ind Health 15:80-93. 1999
  3. ncbi request reprint Environmental antiandrogens: low doses of the fungicide vinclozolin alter sexual differentiation of the male rat
    L E Gray
    Reproductive Toxicology Division, National Health and Environmental Effects Research Laboratory, US Environmental Protection Agency, Research Triangle Park, NC 27711, USA
    Toxicol Ind Health 15:48-64. 1999
  4. ncbi request reprint The estrogenic and antiandrogenic pesticide methoxychlor alters the reproductive tract and behavior without affecting pituitary size or LH and prolactin secretion in male rats
    L E Gray
    Endocrinology Branch, United States Environmental Protection Agency, Research Triangle Park, NC 27111, USA
    Toxicol Ind Health 15:37-47. 1999
  5. ncbi request reprint Tiered screening and testing strategy for xenoestrogens and antiandrogens
    L E Gray
    Endocrinology Branch, RTD, NHEERL, USEPA, Research Triangle Park, NC 27711, USA
    Toxicol Lett 102:677-80. 1998
  6. ncbi request reprint Xenoendocrine disrupters: laboratory studies on male reproductive effects
    L E Gray
    Reproductive Toxicology Division, NHEERL, US Environmental Protection Agency, Research Triangle Park, NC 27711, USA
    Toxicol Lett 102:331-5. 1998
  7. pmc In utero exposure to an AR antagonist plus an inhibitor of fetal testosterone synthesis induces cumulative effects on F1 male rats
    A K Hotchkiss
    Reproductive Toxicology Branch, TAD, NHEERL, ORD, US Environmental Protection Agency, Research Triangle Park, NC 27711, USA
    Reprod Toxicol 30:261-70. 2010
  8. ncbi request reprint Vinclozolin does not alter progesterone receptor (PR) function in vivo despite inhibition of PR binding by its metabolites in vitro
    S C Laws
    Endocrinology Branch, National Health and Environmental Effects Research Laboratory, U S Environmental Protection Agency, Research Triangle Park, North Carolina 27711, USA
    Toxicology 112:173-82. 1996
  9. ncbi request reprint Development of two androgen receptor assays using adenoviral transduction of MMTV-luc reporter and/or hAR for endocrine screening
    P C Hartig
    Reproductive Toxicology Division, U S Environmental Protection Agency, Research Triangle Park, North Carolina 27711, USA
    Toxicol Sci 66:82-90. 2002
  10. ncbi request reprint Vinclozolin and p,p'-DDE alter androgen-dependent gene expression: in vivo confirmation of an androgen receptor-mediated mechanism
    W R Kelce
    National Health and Environmental Effects Research Laboratory, U S Environmental Protection Agency, Research Triangle Park, North Carolina 27711, USA
    Toxicol Appl Pharmacol 142:192-200. 1997

Detail Information

Publications32

  1. ncbi request reprint Administration of potentially antiandrogenic pesticides (procymidone, linuron, iprodione, chlozolinate, p,p'-DDE, and ketoconazole) and toxic substances (dibutyl- and diethylhexyl phthalate, PCB 169, and ethane dimethane sulphonate) during sexual differen
    C Wolf
    Endocrinology Branch, National Health and Environmental Effects Research Laboratory, US Environmental Protection Agency, MD 72, Research Triangle Park, NC 27711, USA
    Toxicol Ind Health 15:94-118. 1999
    ..Only in depth in vitro studies will reveal the degree to which one can rely upon in vivo studies, like those presented here, to predict the cellular and molecular mechanisms of developmental toxicity...
  2. ncbi request reprint The fungicide procymidone alters sexual differentiation in the male rat by acting as an androgen-receptor antagonist in vivo and in vitro
    J Ostby
    Endocrinology Branch, National Health and Environmental Effects Research Laboratory, US EPA, Research Triangle Park, NC 27711, USA
    Toxicol Ind Health 15:80-93. 1999
    ....
  3. ncbi request reprint Environmental antiandrogens: low doses of the fungicide vinclozolin alter sexual differentiation of the male rat
    L E Gray
    Reproductive Toxicology Division, National Health and Environmental Effects Research Laboratory, US Environmental Protection Agency, Research Triangle Park, NC 27711, USA
    Toxicol Ind Health 15:48-64. 1999
    ....
  4. ncbi request reprint The estrogenic and antiandrogenic pesticide methoxychlor alters the reproductive tract and behavior without affecting pituitary size or LH and prolactin secretion in male rats
    L E Gray
    Endocrinology Branch, United States Environmental Protection Agency, Research Triangle Park, NC 27111, USA
    Toxicol Ind Health 15:37-47. 1999
    ..To our knowledge, these data provide the first in vivo example of such a pronounced degree of target tissue selectivity to an environmental endocrine-disrupting chemical...
  5. ncbi request reprint Tiered screening and testing strategy for xenoestrogens and antiandrogens
    L E Gray
    Endocrinology Branch, RTD, NHEERL, USEPA, Research Triangle Park, NC 27711, USA
    Toxicol Lett 102:677-80. 1998
    ..While in utero assays are critical for testing, presently they are not included in screening because they can be relatively long-term studies...
  6. ncbi request reprint Xenoendocrine disrupters: laboratory studies on male reproductive effects
    L E Gray
    Reproductive Toxicology Division, NHEERL, US Environmental Protection Agency, Research Triangle Park, NC 27711, USA
    Toxicol Lett 102:331-5. 1998
    ..Prenatal administration of an Ah receptor agonist (2,3,7,8-TCDD or PCB 169) produces a different spectrum of effects including reduced ejaculated sperm numbers in male rats...
  7. pmc In utero exposure to an AR antagonist plus an inhibitor of fetal testosterone synthesis induces cumulative effects on F1 male rats
    A K Hotchkiss
    Reproductive Toxicology Branch, TAD, NHEERL, ORD, US Environmental Protection Agency, Research Triangle Park, NC 27711, USA
    Reprod Toxicol 30:261-70. 2010
    ....
  8. ncbi request reprint Vinclozolin does not alter progesterone receptor (PR) function in vivo despite inhibition of PR binding by its metabolites in vitro
    S C Laws
    Endocrinology Branch, National Health and Environmental Effects Research Laboratory, U S Environmental Protection Agency, Research Triangle Park, North Carolina 27711, USA
    Toxicology 112:173-82. 1996
    ..In addition, these studies demonstrate the importance of correlating in vitro receptor binding data with in vivo studies in order to understand the physiological consequences of exposure to environmental toxicants...
  9. ncbi request reprint Development of two androgen receptor assays using adenoviral transduction of MMTV-luc reporter and/or hAR for endocrine screening
    P C Hartig
    Reproductive Toxicology Division, U S Environmental Protection Agency, Research Triangle Park, North Carolina 27711, USA
    Toxicol Sci 66:82-90. 2002
    ..Adenovirus transduction for EDC screening has the potential to be employed in a high-throughput mode, and could easily be applied to other cell lines and utilized to deliver other receptors and reporter genes...
  10. ncbi request reprint Vinclozolin and p,p'-DDE alter androgen-dependent gene expression: in vivo confirmation of an androgen receptor-mediated mechanism
    W R Kelce
    National Health and Environmental Effects Research Laboratory, U S Environmental Protection Agency, Research Triangle Park, North Carolina 27711, USA
    Toxicol Appl Pharmacol 142:192-200. 1997
    ..Taken together, these data indicate that vinclozolin and p,p'-DDE act as antiandrogens in vivo by altering the expression of androgen-dependent genes...
  11. ncbi request reprint Substitution of synthetic chimpanzee androgen receptor for human androgen receptor in competitive binding and transcriptional activation assays for EDC screening
    P C Hartig
    Reproductive Toxicology Division, 2525 East Highway 54, MD 72, USEPA, ORD, NHEERL, RTP, NC 27711, United States
    Toxicol Lett 174:89-97. 2007
    ..This suggests that the chimpanzee gene could substitute for the human gene in endocrine screening assays...
  12. ncbi request reprint Perinatal exposure to the phthalates DEHP, BBP, and DINP, but not DEP, DMP, or DOTP, alters sexual differentiation of the male rat
    L E Gray
    Endocrinology Branch, MD 72, U S Environmental Protection Agency, National Health and Environmental Effects Research Laboratory, Reproductive Toxicology Division, Research Triangle Park, North Carolina 27711, USA
    Toxicol Sci 58:350-65. 2000
    ..In summary, DEHP, BBP, and DINP all altered sexual differentiation, whereas DOTP, DEP, and DMP were ineffective at this dose. Whereas DEHP and BBP were of equivalent potency, DINP was about an order of magnitude less active...
  13. ncbi request reprint Effects of environmental antiandrogens on reproductive development in experimental animals
    L E Gray
    Endocrinology Branch, RTD, NHEERL, ORD, USEPA, RTP, NC, 27711, USA
    Hum Reprod Update 7:248-64. 2001
    ..Knowledge of the tissue concentrations of the active toxicants also would provide an important link to in-vitro studies, which provide more useful mechanistic information when they are executed at relevant concentrations...
  14. ncbi request reprint The plasticizer diethylhexyl phthalate induces malformations by decreasing fetal testosterone synthesis during sexual differentiation in the male rat
    L G Parks
    Endocrinology Branch, MD 72, U S Environmental Protection Agency, National Health and Environmental Effects Research Laboratory, Reproductive Toxicology Division, Research Triangle Park, North Carolina 27711, USA
    Toxicol Sci 58:339-49. 2000
    ..These data indicate that DEHP disrupts male rat sexual differentiation by reducing T to female levels in the fetal male rat during a critical stage of reproductive tract differentiation...
  15. pmc Cumulative effects of in utero administration of mixtures of reproductive toxicants that disrupt common target tissues via diverse mechanisms of toxicity
    C V Rider
    MD 72, Reproductive Toxicology Branch, T A Division, NHEERL, ORD, US Environmental Protection Agency, RTP, NC 27711, USA
    Int J Androl 33:443-62. 2010
    ....
  16. doi request reprint High throughput adjustable 96-well plate assay for androgen receptor binding: a practical approach for EDC screening using the chimpanzee AR
    P C Hartig
    Reproductive Toxicology Division, 2525 East Highway 54, MD 72, USEPA, ORD, NHEERL, RTP, NC 27711, USA
    Toxicol Lett 181:126-31. 2008
    ..We provide a very detailed description of the assay protocol itself, and similarly detailed method for producing and solubilizing the receptor...
  17. ncbi request reprint Xenoendocrine disrupters-tiered screening and testing: filling key data gaps
    L E Gray
    Endocrinology Branch, Reproductive Toxicology Division, National Health Environmental Effects Research Laboratory, ORD, US Environmental Protection Agency, MD 72, Research Triangle Park, NC 27111, USA
    Toxicology 181:371-82. 2002
    ..Tier 1 and 2 also should examine relevant mixtures of EDCs. Toxicants that induce malformations in AR-dependent tissues produce cumulative effects even when two chemicals act via different mechanisms of action...
  18. ncbi request reprint The effects of prenatal administration of azo dyes on testicular development in the mouse: a structure activity profile of dyes derived from benzidine, dimethylbenzidine, or dimethoxybenzidine
    L E Gray
    Developmental Reproductive Biology Section, RTB, DTD, USEPA, Research Triangle Park, North Carolina 27711
    Fundam Appl Toxicol 20:177-83. 1993
    ..When CBE was administered on Days 8-10 of gestation it reduced testis weight after puberty by 30%, while treatment after Day 13 did not affect testicular function.(ABSTRACT TRUNCATED AT 250 WORDS)..
  19. ncbi request reprint Antiandrogens as environmental endocrine disruptors
    W R Kelce
    Reproductive Toxicology Division, National Health and Environmental Effects Research Laboratory, US Environmental Protection Agency, Research Triangle Park, NC 27711, USA
    Reprod Fertil Dev 10:105-11. 1998
    ..These chemicals commonly bind androgen receptor with moderate affinity and act as antagonists by inhibiting transcription of androgen dependent genes...
  20. ncbi request reprint In utero exposure to the environmental androgen trenbolone masculinizes female Sprague-Dawley rats
    A K Hotchkiss
    Reproductive Toxicology Division, Endocrinology Branch, MD 72, National Health and Environmental Effects Research Laboratory, US Environmental Protection Agency, Research Triangle Park, NC 27711, USA
    Toxicol Lett 174:31-41. 2007
    ..5mg/day, 1.0mg/day, or 2.0mg/day groups. These changes were associated with amniotic fluid concentrations of TB that compare favorably with concentrations known to be active in both in vitro systems and in fish...
  21. ncbi request reprint In vivo and in vitro anti-androgenic effects of DE-71, a commercial polybrominated diphenyl ether (PBDE) mixture
    T E Stoker
    MD 72, Endocrinology Branch, Reproductive Toxicology Division, National Health and Environmental Effects Research Laboratory, Office of Research and Development, United States EPA, Research Triangle Park, NC 27711, USA
    Toxicol Appl Pharmacol 207:78-88. 2005
    ....
  22. ncbi request reprint Use of the laboratory rat as a model in endocrine disruptor screening and testing
    L E Gray
    Reproductive Toxicology Division, US Environmental Protection Agency, Research Triangle Park, NC, USA
    ILAR J 45:425-37. 2004
    ....
  23. ncbi request reprint Masculinization of female mosquitofish in Kraft mill effluent-contaminated Fenholloway River water is associated with androgen receptor agonist activity
    L G Parks
    Endocrinology Branch, National Health and Environmental Effects Research Laboratory (NHEERL, USEPA, RTD, Research Triangle Park, NC 27711, USA
    Toxicol Sci 62:257-67. 2001
    ..In summary, water collected downstream of the Kraft mill on the Fenholloway River contains unidentified androgenic substances whose presence is associated with masculinization of female mosquitofish...
  24. ncbi request reprint Effects of pesticides and toxic substances on behavioral and morphological reproductive development: endocrine versus nonendocrine mechanisms
    L E Gray
    Endocrinology Branch, US Environmental Protection Agency, Research Triangle Park, NC 27711, USA
    Toxicol Ind Health 14:159-84. 1998
    ....
  25. ncbi request reprint Ligand-based identification of environmental estrogens
    C L Waller
    Experimental Toxicology Divisions, United States Environmental Protection Agency, Research Triangle Park, North Carolina 27711, USA
    Chem Res Toxicol 9:1240-8. 1996
    ..The general application of this 3D-QSAR model within a toxicological framework is, at present, limited only by the quantity and quality of biological data for relevant biomarkers of toxicity and hormonal responsiveness...
  26. ncbi request reprint In vitro and in vivo effects of 17beta-trenbolone: a feedlot effluent contaminant
    Vickie S Wilson
    US Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Reproductive Toxicology Division, MD 72, Research Triangle Park, North Carolina 27711, USA
    Toxicol Sci 70:202-11. 2002
    ..In summary, TB is a potent environmental androgen both in vitro and in vivo and, in contrast to other reports, can induce developmental abnormalities in the fetus...
  27. pmc Research needs for the risk assessment of health and environmental effects of endocrine disruptors: a report of the U.S. EPA-sponsored workshop
    R J Kavlock
    National Health and Environmental Effects Research Laboratory, U S EPA, Research Triangle Park, NC 27711, USA
    Environ Health Perspect 104:715-40. 1996
    ..This report summarizes the discussions of the work groups and details the recommendations for additional research...
  28. ncbi request reprint A novel cell line, MDA-kb2, that stably expresses an androgen- and glucocorticoid-responsive reporter for the detection of hormone receptor agonists and antagonists
    Vickie S Wilson
    U S Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Reproductive Toxicology Division, MD 72, Research Triangle Park, North Carolina 27711, USA
    Toxicol Sci 66:69-81. 2002
    ..In summary, we have developed a cell line that can be used to screen chemicals, not just for AR- but for GR-mediated activities as well...
  29. ncbi request reprint Androgen receptor antagonism by the organophosphate insecticide fenitrothion
    H Tamura
    Department of Applied Biochemistry, Meijo University, Nagoya 468-8502, Japan
    Toxicol Sci 60:56-62. 2001
    ....
  30. ncbi request reprint A mixture of the "antiandrogens" linuron and butyl benzyl phthalate alters sexual differentiation of the male rat in a cumulative fashion
    A K Hotchkiss
    Deparment of Zoology, North Carolina State University, Raleigh, North Carolina 27695, USA
    Biol Reprod 71:1852-61. 2004
    ..Taken together, these data provide additional evidence of cumulative effects of antiandrogen mixtures on male reproductive development...
  31. ncbi request reprint An environmental antiandrogen, vinclozolin, alters the organization of play behavior
    A K Hotchkiss
    Department of Zoology, North Carolina State University, Raleigh, NC 27695, USA
    Physiol Behav 79:151-6. 2003
    ..Hence, this study suggests that perinatal exposure to vinclozolin, an environmental antiandrogen, can alter androgen-dependent behavior, such as play, in the male rat...
  32. pmc Androgens and environmental antiandrogens affect reproductive development and play behavior in the Sprague-Dawley rat
    A K Hotchkiss
    Department of Zoology, North Carolina State University, Raleigh, North Carolina, USA
    Environ Health Perspect 110:435-9. 2002
    ..Therefore, this study demonstrates that perinatal exposure to vinclozolin, an environmental antiandrogen, can alter androgen-dependent play behavior in the male rat...