G T Ankley

Summary

Affiliation: U.S. Environmental Protection Agency
Country: USA

Publications

  1. ncbi request reprint Assessment of environmental stressors potentially responsible for malformations in North American anuran amphibians
    Gerald T Ankley
    Mid Continent Ecology Division, National Health and Environmental Effects Research Laboratory, Office of Research and Development, US Environmental Protection Agency, 6201 Congdon Boulevard, Duluth, MN 55804 2595, USA
    Ecotoxicol Environ Saf 58:7-16. 2004
  2. pmc Discovery and validation of gene classifiers for endocrine-disrupting chemicals in zebrafish (danio rerio)
    Rong Lin Wang
    USEPA, Ecological Exposure Research Division, National Exposure Research Laboratory, 26 W Martin Luther King Dr, Cincinnati, OH 45268, USA
    BMC Genomics 13:358. 2012
  3. doi request reprint Cross-species conservation of endocrine pathways: a critical analysis of tier 1 fish and rat screening assays with 12 model chemicals
    Gerald T Ankley
    U S Environmental Protection Agency, Office of Research and Development, Duluth, Minnesota, USA
    Environ Toxicol Chem 32:1084-7. 2013
  4. ncbi request reprint Repeating history: pharmaceuticals in the environment
    Gerald T Ankley
    U S EPA, USA
    Environ Sci Technol 41:8211-7. 2007
  5. ncbi request reprint Reproductive and developmental toxicity and bioconcentration of perfluorooctanesulfonate in a partial life-cycle test with the fathead minnow (Pimephales promelas)
    Gerald T Ankley
    Mid Continent Ecology Division, National Health and Environmental Effects Research Laboratory, U S Environmental Protection Agency, 6201 Congdon Boulevard, Duluth, Minnesota 55804, USA
    Environ Toxicol Chem 24:2316-24. 2005
  6. ncbi request reprint Effects of two fungicides with multiple modes of action on reproductive endocrine function in the fathead minnow (Pimephales promelas)
    Gerald T Ankley
    U S Environmental Protection Agency, ORD, NHEERL, Mid Continent Ecology Division, Duluth, Minnesota 55804, USA
    Toxicol Sci 86:300-8. 2005
  7. doi request reprint Relationship of plasma sex steroid concentrations in female fathead minnows to reproductive success and population status
    Gerald T Ankley
    US Environmental Protection Agency, Mid Continent Ecology Division, 6201 Congdon Boulevard, Duluth, MN 55804 USA
    Aquat Toxicol 88:69-74. 2008
  8. ncbi request reprint Effects of the androgenic growth promoter 17-beta-trenbolone on fecundity and reproductive endocrinology of the fathead minnow
    Gerald T Ankley
    U S Environmental Protection Agency, National Health and Environmental Effects Research Laboratory, Mid Continent Ecology Division, 6201 Congdon Boulevard, Duluth, Minnesota 55804, USA
    Environ Toxicol Chem 22:1350-60. 2003
  9. doi request reprint Adverse outcome pathways: a conceptual framework to support ecotoxicology research and risk assessment
    Gerald T Ankley
    U S Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Mid Continent Ecology Division, Duluth, Minnesota 55804, USA
    Environ Toxicol Chem 29:730-41. 2010
  10. doi request reprint Dynamic nature of alterations in the endocrine system of fathead minnows exposed to the fungicide prochloraz
    Gerald T Ankley
    Mid Continent Ecology Division, National Health and Environmental Effects Research Laboratory, Office of Research and Development, U S Environmental Protection Agency, Duluth, Minnesota 55804, USA
    Toxicol Sci 112:344-53. 2009

Detail Information

Publications87

  1. ncbi request reprint Assessment of environmental stressors potentially responsible for malformations in North American anuran amphibians
    Gerald T Ankley
    Mid Continent Ecology Division, National Health and Environmental Effects Research Laboratory, Office of Research and Development, US Environmental Protection Agency, 6201 Congdon Boulevard, Duluth, MN 55804 2595, USA
    Ecotoxicol Environ Saf 58:7-16. 2004
    ....
  2. pmc Discovery and validation of gene classifiers for endocrine-disrupting chemicals in zebrafish (danio rerio)
    Rong Lin Wang
    USEPA, Ecological Exposure Research Division, National Exposure Research Laboratory, 26 W Martin Luther King Dr, Cincinnati, OH 45268, USA
    BMC Genomics 13:358. 2012
    ..Candidate classifiers were evaluated by gene set enrichment analysis (GSEA) on both the original training data and a dedicated validation dataset...
  3. doi request reprint Cross-species conservation of endocrine pathways: a critical analysis of tier 1 fish and rat screening assays with 12 model chemicals
    Gerald T Ankley
    U S Environmental Protection Agency, Office of Research and Development, Duluth, Minnesota, USA
    Environ Toxicol Chem 32:1084-7. 2013
    ..The observations are significant both to the cross-species extrapolation of toxicity of HPG-active substances and the optimization of screening and testing frameworks for endocrine-disrupting chemicals...
  4. ncbi request reprint Repeating history: pharmaceuticals in the environment
    Gerald T Ankley
    U S EPA, USA
    Environ Sci Technol 41:8211-7. 2007
  5. ncbi request reprint Reproductive and developmental toxicity and bioconcentration of perfluorooctanesulfonate in a partial life-cycle test with the fathead minnow (Pimephales promelas)
    Gerald T Ankley
    Mid Continent Ecology Division, National Health and Environmental Effects Research Laboratory, U S Environmental Protection Agency, 6201 Congdon Boulevard, Duluth, Minnesota 55804, USA
    Environ Toxicol Chem 24:2316-24. 2005
    ....
  6. ncbi request reprint Effects of two fungicides with multiple modes of action on reproductive endocrine function in the fathead minnow (Pimephales promelas)
    Gerald T Ankley
    U S Environmental Protection Agency, ORD, NHEERL, Mid Continent Ecology Division, Duluth, Minnesota 55804, USA
    Toxicol Sci 86:300-8. 2005
    ..These studies contribute to a better mechanistic understanding of the extrapolation of effects of endocrine-disrupting chemicals across vertebrate classes...
  7. doi request reprint Relationship of plasma sex steroid concentrations in female fathead minnows to reproductive success and population status
    Gerald T Ankley
    US Environmental Protection Agency, Mid Continent Ecology Division, 6201 Congdon Boulevard, Duluth, MN 55804 USA
    Aquat Toxicol 88:69-74. 2008
    ..Results of our analysis illustrate a conceptual framework whereby a commonly measured biomarker, sex steroid status, could be linked to individual- and population-level effects in fish...
  8. ncbi request reprint Effects of the androgenic growth promoter 17-beta-trenbolone on fecundity and reproductive endocrinology of the fathead minnow
    Gerald T Ankley
    U S Environmental Protection Agency, National Health and Environmental Effects Research Laboratory, Mid Continent Ecology Division, 6201 Congdon Boulevard, Duluth, Minnesota 55804, USA
    Environ Toxicol Chem 22:1350-60. 2003
    ..Given the widespread use of trenbolone acetate as a growth promoter, and relative stability of its metabolites in animal wastes, further studies are warranted to assess potential ecological risk...
  9. doi request reprint Adverse outcome pathways: a conceptual framework to support ecotoxicology research and risk assessment
    Gerald T Ankley
    U S Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Mid Continent Ecology Division, Duluth, Minnesota 55804, USA
    Environ Toxicol Chem 29:730-41. 2010
    ..In the concluding sections of the paper, we discuss how AOPs can help to guide research that supports chemical risk assessments and advocate for the incorporation of this approach into a broader systems biology framework...
  10. doi request reprint Dynamic nature of alterations in the endocrine system of fathead minnows exposed to the fungicide prochloraz
    Gerald T Ankley
    Mid Continent Ecology Division, National Health and Environmental Effects Research Laboratory, Office of Research and Development, U S Environmental Protection Agency, Duluth, Minnesota 55804, USA
    Toxicol Sci 112:344-53. 2009
    ..These studies demonstrate the adaptability of the HPG axis to chemical stress and highlight the need to consider the dynamic nature of the system when developing approaches to assess potential risks of endocrine-active chemicals...
  11. ncbi request reprint Evaluation of the model anti-androgen flutamide for assessing the mechanistic basis of responses to an androgen in the fathead minnow (Pimephales promelas)
    Gerald T Ankley
    US Environmental Protection Agency, Mid Continent Ecology Division, Duluth, Minnesota 55804, USA
    Environ Sci Technol 38:6322-7. 2004
    ..The results of these studies provide data demonstrating the validity of using the fathead minnow as a model species for detecting EDCs that exert toxicity through interactions with the AR...
  12. ncbi request reprint Partial life-cycle toxicity and bioconcentration modeling of perfluorooctanesulfonate in the northern leopard frog (Rana pipiens)
    Gerald T Ankley
    US Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Mid Continent Ecology Division, Duluth, Minnesota 55804, USA
    Environ Toxicol Chem 23:2745-55. 2004
    ..Overall, our studies suggest that R. pipiens is not exceptionally sensitive to PFOS in terms of either direct toxicity or bioconcentration potential of the chemical...
  13. doi request reprint Use of chemical mixtures to differentiate mechanisms of endocrine action in a small fish model
    Gerald T Ankley
    U S Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Mid Continent Ecology Division, Duluth, MN 55804, USA
    Aquat Toxicol 99:389-96. 2010
    ....
  14. ncbi request reprint The fathead minnow in aquatic toxicology: past, present and future
    Gerald T Ankley
    US Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Mid Continent Ecology Division, Duluth, MN 55804, USA
    Aquat Toxicol 78:91-102. 2006
    ....
  15. doi request reprint Endocrine disrupting chemicals in fish: developing exposure indicators and predictive models of effects based on mechanism of action
    Gerald T Ankley
    USEPA, National Health and Environmental Effects Research Lab, Duluth, MN, USA
    Aquat Toxicol 92:168-78. 2009
    ..Although our project is focused specifically on EDCs in fish, we believe that the basic conceptual approach has utility for systematically assessing exposure and effects of chemicals with other MOA across a variety of biological systems...
  16. ncbi request reprint Evaluation of the aromatase inhibitor fadrozole in a short-term reproduction assay with the fathead minnow (Pimephales promelas)
    Gerald T Ankley
    U S Environmental Protection Agency, National Health and Environmental Effects Research Laboratory, Mid Continent Ecology Division, Duluth, Minnesota 55804, USA
    Toxicol Sci 67:121-30. 2002
    ....
  17. ncbi request reprint Reactivity profiles of ligands of mammalian retinoic acid receptors: a preliminary COREPA analysis
    G T Ankley
    US Environmental Protection Agency, National Health and Environmental Effects Research Laboratory, Mid Continent Ecology Division, Duluth, MN 55804, USA
    SAR QSAR Environ Res 13:365-77. 2002
    ..Variations in reactivity profiles for the RAR versus RXR families would be consistent with established differences in their affinity for endogenous retinoids, likely reflecting functional differences in the receptors...
  18. pmc Toxicogenomics in regulatory ecotoxicology
    Gerald T Ankley
    U S EPA
    Environ Sci Technol 40:4055-65. 2006
  19. ncbi request reprint Assessment of the risk of solar ultraviolet radiation to amphibians. I. Dose-dependent induction of hindlimb malformations in the northern leopard frog (Rana pipiens)
    Gerald T Ankley
    U S Environmental Protection Agency, National Health and Environmental Effects Research Laboratory, Mid Continent Ecology Division, Duluth, Minnesota 55804, USA
    Environ Sci Technol 36:2853-8. 2002
    ..The results of this study, in conjunction with measured or predicted exposure data from natural settings, provide a basis for quantitative prediction of the risk of solar UV radiation to amphibians...
  20. doi request reprint Temporal evaluation of effects of a model 3β-hydroxysteroid dehydrogenase inhibitor on endocrine function in the fathead minnow
    Gerald T Ankley
    US Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Lab, Duluth, Minnesota, USA
    Environ Toxicol Chem 30:2094-102. 2011
    ..This information is important to the design and interpretation of approaches for assessing the occurrence and effects of HPG-active chemicals in both the laboratory and the field...
  21. ncbi request reprint Ketoconazole in the fathead minnow (Pimephales promelas): reproductive toxicity and biological compensation
    Gerald T Ankley
    U S Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Mid Continent Ecology Division, 6201 Congdon Boulevard, Duluth, Minnesota 55804, USA
    Environ Toxicol Chem 26:1214-23. 2007
    ..This has important implications for the interpretation of data from tests with endocrine-active chemicals...
  22. ncbi request reprint Aspects of basic reproductive biology and endocrinology in the fathead minnow (Pimephales promelas)
    K M Jensen
    U S Environmental Protection Agency, Mid Continent Ecology Division, 6201 Congdon Boulevard, Duluth, MN 55804, USA
    Comp Biochem Physiol C Toxicol Pharmacol 128:127-41. 2001
    ..Concentrations of beta-estradiol in females and 11-ketotestosterone in males were positively correlated with testosterone concentrations...
  23. ncbi request reprint Description and evaluation of a short-term reproduction test with the fathead minnow (Pimephales promelas)
    G T Ankley
    U S Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Mid Continent Ecology Division, 6201 Congdon Boulevard, Duluth, Minnesota 55804, USA
    Environ Toxicol Chem 20:1276-90. 2001
    ..These studies demonstrate the utility of this short-term assay for identifying chemicals that exert reproductive toxicity through alterations in endocrine systems controlled by estrogens and androgens...
  24. ncbi request reprint Stage- and species-specific developmental toxicity of all-trans retinoic acid in four native North American ranids and Xenopus laevis
    S J Degitz
    U S Environmental Protection Agency, Mid Continent Ecology Division, 6201 Congdon Boulevard, Duluth, Minnesota 55804, USA
    Toxicol Sci 57:264-74. 2000
    ..The significance of these findings is discussed in the context of the possible occurrence of retinoid mimics in the environment...
  25. ncbi request reprint Ambient solar UV radiation causes mortality in larvae of three species of Rana under controlled exposure conditions
    J E Tietge
    Mid Continent Ecology Division, U S Environmental Protection Agency, 6201 Congdon Blvd, Duluth, MN 55804, USA
    Photochem Photobiol 74:261-8. 2001
    ....
  26. ncbi request reprint Masculinization of female mosquitofish in Kraft mill effluent-contaminated Fenholloway River water is associated with androgen receptor agonist activity
    L G Parks
    Endocrinology Branch, National Health and Environmental Effects Research Laboratory (NHEERL, USEPA, RTD, Research Triangle Park, NC 27711, USA
    Toxicol Sci 62:257-67. 2001
    ..In summary, water collected downstream of the Kraft mill on the Fenholloway River contains unidentified androgenic substances whose presence is associated with masculinization of female mosquitofish...
  27. ncbi request reprint Comparing the effects of stage and duration of retinoic Acid exposure on amphibian limb development: chronic exposure results in mortality, not limb malformations
    Sigmund J Degitz
    U S Environmental Protection Agency, Mid Continent Ecology Division, 6201 Congdon Boulevard, Duluth, Minnesota 55804, USA
    Toxicol Sci 74:139-46. 2003
    ..From these experiments and our previous work, we conclude that it is unlikely that retinoid mimics would produce the spectrum of limb malformations which recently have been observed in amphibians collected from the field...
  28. ncbi request reprint Small fish models for identifying and assessing the effects of endocrine-disrupting chemicals
    Gerald T Ankley
    Mid Continent Ecology Division, US Environmental Protection Agency Office of Research and Development, National Health and Environmental Effects Research Laboratory, Duluth, MN, USA
    ILAR J 45:469-83. 2004
    ..Examples of results with specific chemicals in tests with the fathead minnow, medaka, and zebrafish are presented and discussed in terms of sensitivity and specificity for different classes of EDCs...
  29. ncbi request reprint Inhibition of retinoid activity by components of a paper mill effluent
    Patrick K Schoff
    Mid Continent Ecology Division, National Health and Environmental Effects Research Laboratory, US Environmental Protection Agency, Duluth, Minnesota 55811, USA
    Environ Pollut 119:1-4. 2002
    ..In a limited fractionation through a C-18 solid phase-exchange column the inhibitory activity was retained in the aqueous fraction, indicating that the activity was polar...
  30. ncbi request reprint In utero exposure to the environmental androgen trenbolone masculinizes female Sprague-Dawley rats
    A K Hotchkiss
    Reproductive Toxicology Division, Endocrinology Branch, MD 72, National Health and Environmental Effects Research Laboratory, US Environmental Protection Agency, Research Triangle Park, NC 27711, USA
    Toxicol Lett 174:31-41. 2007
    ..5mg/day, 1.0mg/day, or 2.0mg/day groups. These changes were associated with amniotic fluid concentrations of TB that compare favorably with concentrations known to be active in both in vitro systems and in fish...
  31. ncbi request reprint NMR analysis of male fathead minnow urinary metabolites: a potential approach for studying impacts of chemical exposures
    D R Ekman
    Ecosystems Research Division, U S EPA, 960 College Station Road, Athens, GA 30605, United States
    Aquat Toxicol 85:104-12. 2007
    ..The results of these investigations clearly demonstrate the potential utility of an NMR-based approach for assessing chemical exposures in male fathead minnow, using urine collected from individual fish...
  32. ncbi request reprint DNA microarray application in ecotoxicology: experimental design, microarray scanning, and factors affecting transcriptional profiles in a small fish species
    Rong Lin Wang
    Ecological Exposure Research Division, National Exposure Research Laboratory, US Environmental Protection Agency, Cincinnati, Ohio 45268, USA
    Environ Toxicol Chem 27:652-63. 2008
    ..These findings will help to improve applications of microarrays in future ecotoxicological studies...
  33. doi request reprint Impacts of an anti-androgen and an androgen/anti-androgen mixture on the metabolite profile of male fathead minnow urine
    Timothy W Collette
    Office of Research and Development, National Exposure Research Laboratory, U S Environmental Protection Agency, 960 College Station Rd, Athens, Georgia 30605, USA
    Environ Sci Technol 44:6881-6. 2010
    ..The relative occurrence of male-like nuptial tubercles in female FHM exposed to TB and to the mixtures of TB and CA supported the metabolomics data...
  34. doi request reprint Characterization of the androgen-sensitive MDA-kb2 cell line for assessing complex environmental mixtures
    Lindsey S Blake
    Integrated Biosciences, University of Minnesota, Duluth, 1035 University Dr, Duluth, Minnesota 55812, USA
    Environ Toxicol Chem 29:1367-76. 2010
    ....
  35. ncbi request reprint Comparison of fathead minnow ovary explant and H295R cell-based steroidogenesis assays for identifying endocrine-active chemicals
    Daniel L Villeneuve
    US Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Mid Continent Ecology Division, 6201 Congdon Blvd, Duluth, MN, 55804, USA
    Ecotoxicol Environ Saf 68:20-32. 2007
    ..Overall, however, results of this study suggest that both the H295R and fathead minnow ovary explant assays have utility for identifying endocrine-active chemicals in screening-type applications...
  36. ncbi request reprint Mechanistic computational model of ovarian steroidogenesis to predict biochemical responses to endocrine active compounds
    Michael S Breen
    National Center for Computational Toxicology, Office of Research and Development, U S Environmental Protection Agency, Research Triangle Park, NC 27711, USA
    Ann Biomed Eng 35:970-81. 2007
    ..This capability could be useful for pharmaceutical development and environmental health assessments with EAC...
  37. ncbi request reprint DNA microarray-based ecotoxicological biomarker discovery in a small fish model species
    Rong Lin Wang
    Ecological Exposure Research Division, National Exposure Research Lab, US Environmental Protection Agency, Cincinnati, Ohio 45268, USA
    Environ Toxicol Chem 27:664-75. 2008
    ..These gene classifiers could serve as potential biomarkers of exposure to specific classes of endocrine disruptors...
  38. doi request reprint Influence of ovarian stage on transcript profiles in fathead minnow (Pimephales promelas) ovary tissue
    Daniel L Villeneuve
    US Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Mid Continent Ecology Division, 6201 Congdon Blvd, Duluth, MN 55804, USA
    Aquat Toxicol 98:354-66. 2010
    ....
  39. doi request reprint Reproductive toxicity of vinclozolin in the fathead minnow: confirming an anti-androgenic mode of action
    Dalma Martinovic
    Mid Continent Ecology Division, National Health and Environmental Effects Research Laboratory, Office of Research and Development, US EPA, Duluth, MN 55804, USA
    Environ Toxicol Chem 27:478-88. 2008
    ....
  40. doi request reprint Hypoxia alters gene expression in the gonads of zebrafish (Danio rerio)
    Dalma Martinovic
    US Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Mid Continent Ecology Division, 6201 Congdon Blvd, Duluth, MN, USA, 55804, USA
    Aquat Toxicol 95:258-72. 2009
    ..g., hydroxysteroid dehydrogenase alterations, downregulation of contractile elements, etc.)...
  41. ncbi request reprint Temporal variation in the estrogenicity of a sewage treatment plant effluent and its biological significance
    Dalma Martinovic
    Department of Fisheries, Wildlife, and Conservation Biology, 1980 Folwell Avenue, University of Minnesota, St Paul, Minnesota 55108, USA
    Environ Sci Technol 42:3421-7. 2008
    ..The variability of the effluent's estrogenicity suggests that studies concerned with the effects of STP effluents should collect multiple daily samples and then test them on an appropriate time-weighted basis...
  42. doi request reprint Altered gene expression in the brain and ovaries of zebrafish (Danio rerio) exposed to the aromatase inhibitor fadrozole: microarray analysis and hypothesis generation
    L Villeneuve
    U S Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Mid Continent Ecology Division, 6201 Congdon Boulevard, Duluth, Minnesosta 55804, USA
    Environ Toxicol Chem 28:1767-82. 2009
    ..These hypotheses and others derived from the microarray results provide a foundation for future studies aimed at understanding responses of the HPG axis to EACs and other chemical stressors...
  43. pmc Direct effects, compensation, and recovery in female fathead minnows exposed to a model aromatase inhibitor
    Daniel L Villeneuve
    U S Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Mid Continent Ecology Division, Duluth, Minnesota 55804, USA
    Environ Health Perspect 117:624-31. 2009
    ..Several chemicals in the environment have the potential to inhibit aromatase, an enzyme critical to estrogen synthesis...
  44. doi request reprint I. Effects of a dopamine receptor antagonist on fathead minnow, Pimephales promelas, reproduction
    Daniel L Villeneuve
    US Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Mid Continent Ecology Division, 6201 Congdon Boulevard, Duluth, MN 55804, USA
    Ecotoxicol Environ Saf 73:472-7. 2010
    ..Results suggest that non-lethal concentrations of haloperidol do not directly impair fish reproduction. Potential effects of haloperidol on reproductive behaviors and gene expression were examined in a companion study...
  45. doi request reprint Effects of a 3beta-hydroxysteroid dehydrogenase inhibitor, trilostane, on the fathead minnow reproductive axis
    Daniel L Villeneuve
    Mid Continent Ecology Division, National Health and Environmental Effects Research Laboratory, US Environmental Protection Agency, Office of Research and Development, 6201 Congdon Boulevard, Duluth, Minnesota 55804, USA
    Toxicol Sci 104:113-23. 2008
    ....
  46. doi request reprint II: Effects of a dopamine receptor antagonist on fathead minnow dominance behavior and ovarian gene expression in the fathead minnow and zebrafish
    Daniel L Villeneuve
    US Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Mid Continent Ecology Division, 6201 Congdon Blvd, Duluth, MN 55804, USA
    Ecotoxicol Environ Saf 73:478-85. 2010
    ..Results suggest that non-lethal concentrations of haloperidol do not induce ovarian molecular responses that could serve as biomarkers of exposure to D2R antagonists, but may impact behavior...
  47. doi request reprint A transcriptomics-based biological framework for studying mechanisms of endocrine disruption in small fish species
    Rong Lin Wang
    USEPA, Ecological Exposure Research Division, National Exposure Research Laboratory, 26 W Martin Luther King Dr, Cincinnati, OH 45268, USA
    Aquat Toxicol 98:230-44. 2010
    ..These candidate networks/pathways have a variety of profound impacts on such cellular functions as stress response, cell cycle, and apoptosis...
  48. ncbi request reprint Linkage of biochemical responses to population-level effects: a case study with vitellogenin in the fathead minnow (Pimephales promelas)
    David H Miller
    U S Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Mid Continent Ecology Division, Grosse Ile, Michigan 48138, USA
    Environ Toxicol Chem 26:521-7. 2007
    ..2% of the preexposed population. Overall, the current study provides an example of how changes in a biomarker (VTG concentration) can be quantitatively translated into adverse effects at the individual and population levels...
  49. ncbi request reprint A graphical systems model to facilitate hypothesis-driven ecotoxicogenomics research on the teleost brain-pituitary-gonadal axis
    Daniel L Villeneuve
    Mid Continent Ecology Division, U S EPA, USA
    Environ Sci Technol 41:321-30. 2007
    ..Furthermore, microarray results helped identify additional elements (genes/ proteins) that could be included in the model to potentially increase its predictive capacity. With proper recognition of..
  50. ncbi request reprint Induction of an estrogen-responsive reporter gene in rainbow trout hepatoma cells (RTH 149) at 11 or 18 degrees C
    Michael W Hornung
    US Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Mid Continent Ecology Division, 6201 Congdon Boulevard, Duluth, Minnesota 55804, USA
    Environ Toxicol Chem 22:866-71. 2003
    ....
  51. ncbi request reprint Relationship between brain and ovary aromatase activity and isoform-specific aromatase mRNA expression in the fathead minnow (Pimephales promelas)
    Daniel L Villeneuve
    US Environmental Protection Agency, Mid Continent Ecology Division, Duluth, MN, USA
    Aquat Toxicol 76:353-68. 2006
    ....
  52. ncbi request reprint Evaluation of androstenedione as an androgenic component of river water downstream of a pulp and paper mill effluent
    Eth J Durhan
    US Environmental Protection Agency, National Health and Environmental Effects Laboratory, Duluth, Minnesota 55804, USA
    Environ Toxicol Chem 21:1973-6. 2002
    ..We conclude that, although androstenedione was present at easily detectable concentrations in the river water (> 100 ng/L), this compound is not associated with androgenic activity of water from the site...
  53. ncbi request reprint Overview of data and conceptual approaches for derivation of quantitative structure-activity relationships for ecotoxicological effects of organic chemicals
    Steven P Bradbury
    U S Environmental Protection Agency, National Health and Environmental Effects Research Laboratory, Mid Continent Ecology Division, 6201 Congdon Boulevard, Duluth, Minnesota 55804, USA
    Environ Toxicol Chem 22:1789-98. 2003
    ..Finally, an overview of expert systems that integrate chemical-specific mode of action classification and associated QSAR selection for estimating potential toxicological effects of organic chemicals is presented...
  54. ncbi request reprint Transcription of key genes regulating gonadal steroidogenesis in control and ketoconazole- or vinclozolin-exposed fathead minnows
    Daniel L Villeneuve
    U S Environmental Protection Agency, ORD, NHEERL, Mid Continent Ecology Division, Duluth, Minnesota 55804, USA
    Toxicol Sci 98:395-407. 2007
    ....
  55. ncbi request reprint Ecotoxicogenomics: linkages between exposure and effects in assessing risks of aquatic contaminants to fish
    Ann L Miracle
    U S Environmental Protection Agency, Ecological Exposure Research Division, Cincinnati, OH, USA
    Reprod Toxicol 19:321-6. 2005
    ..The challenges of the individual technologies and the combinations of tools for ecotoxicogenomics are discussed in application to aquatic toxicology with a particular emphasis on fish testing...
  56. ncbi request reprint Cloning and in vitro expression and characterization of the androgen receptor and isolation of estrogen receptor alpha from the fathead Minnow (Pimephales promelas)
    Vickie S Wilson
    US Environmental Protection Agency, ORD, NHEERL, Reproductive Toxicology Division, North Carolina 27711, USA
    Environ Sci Technol 38:6314-21. 2004
    ..Future plans include comparison of binding affinities of the fhAR to those of the human AR, also expressed in COS cells, using a range of EDCs...
  57. ncbi request reprint Mechanistic basis for estrogenic effects in fathead minnow (Pimephales promelas) following exposure to the androgen 17alpha-methyltestosterone: conversion of 17alpha-methyltestosterone to 17alpha-methylestradiol
    Michael W Hornung
    United States Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Mid Continent Ecology Division, Duluth, MN 55804, USA
    Aquat Toxicol 66:15-23. 2004
    ..3% of E2, support the hypothesis that aromatization of MT to ME2 contributes to the estrogenic effects in fathead minnows following exposure to this androgen...
  58. ncbi request reprint Characterization of responses to the antiandrogen flutamide in a short-term reproduction assay with the fathead minnow
    Kathleen M Jensen
    U S Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Mid Continent Ecology Division, 6201 Congdon Boulevard, Duluth, MN 55804 USA
    Aquat Toxicol 70:99-110. 2004
    ..Consequently, our findings suggest that the 21-day reproduction assay utilizing fathead minnows is a sensitive short-term screening method for the detection of endocrine-disrupting chemicals, including antiandrogens...
  59. ncbi request reprint Modeling impacts on populations: fathead minnow (Pimephales promelas) exposure to the endocrine disruptor 17beta-trenbolone as a case study
    David H Miller
    Mid Continent Ecology Division, National Health and Environmental Effects Research Laboratory, US Environmental Protection Agency, 9311 Groh Rd, Grosse Ile, MI 48138, USA
    Ecotoxicol Environ Saf 59:1-9. 2004
    ..Overall, fathead minnow populations exposed to continued concentrations of 17beta-trenbolone equal to or greater than 0.027 microg/L were projected to have average equilibrium population sizes that approached zero...
  60. ncbi request reprint Comparison of relative binding affinities of endocrine active compounds to fathead minnow and rainbow trout estrogen receptors
    Jeffrey S Denny
    U S Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Mid Continent Ecology Division, Duluth, Minnesota 55804, USA
    Environ Toxicol Chem 24:2948-53. 2005
    ..Comparison of RBAs of the chemicals tested for fhmER and rbtER indicates that the rank order of RBAs essentially are the same for both species...
  61. ncbi request reprint Adverse effects of environmental antiandrogens and androgens on reproductive development in mammals
    L Earl Gray
    MD 72, Endocrinology Branch, Reproductive Toxicology Division, NHEERL, ORD, US Environmental Protection Agency, RTP, NC 27771, USA
    Int J Androl 29:96-104; discussion 105-8. 2006
    ....
  62. ncbi request reprint Evaluation of a commercial kit for measuring vitellogenin in the fathead minnow (Pimephales promelas)
    Kathleen M Jensen
    National Health and Environmental Effects Research Laboratory, Office of Research and Development, Mid Continent Ecology Division, US Environmental Protection Agency, 6201 Congdon Boulevard, Duluth, MN 55804, USA
    Ecotoxicol Environ Saf 64:101-5. 2006
    ..Results obtained using the two different ELISA methods were consistently similar...
  63. ncbi request reprint Development of quantitative real-time PCR assays for fathead minnow (Pimephales promelas) gonadotropin beta subunit mRNAs to support endocrine disruptor research
    Daniel L Villeneuve
    United States Environmental Protection Agency, ORD, NHEERL, Mid Continent Ecology Division, 6201 Congdon Blvd, Duluth, MN 55803, USA
    Comp Biochem Physiol C Toxicol Pharmacol 145:171-83. 2007
    ..Results of such experiments should facilitate a greater understanding of integrated system-wide responses of the fathead minnow brain-pituitary-gonadal axis to stressors including EDCs...
  64. ncbi request reprint Evaluation of the methoxytriazine herbicide prometon using a short-term fathead minnow reproduction test and a suite of in vitro bioassays
    Daniel L Villeneuve
    US Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Mid Continent Ecology Divison, 6201 Congdon Boulevard, Duluth, Minnesota, USA
    Environ Toxicol Chem 25:2143-53. 2006
    ..The relevance of these effects to hazard assessment for the pesticide is uncertain...
  65. ncbi request reprint Uptake and metabolism of all-trans retinoic acid by three native north American ranids
    Patricia A Kosian
    US EPA, National Health and Environmental Effects Research Lab, Mid Continent Ecology Division, Duluth, Minnesota 55804, USA
    Toxicol Sci 74:147-56. 2003
    ....
  66. ncbi request reprint Developmental toxicity of methoprene and several degradation products in Xenopus laevis
    Sigmund J Degitz
    US Environmental Protection Agency, National Health and Environmental Effects Research Laboratory, Mid Continent Ecology Division, 6201 Congdon Boulevard, Duluth, MN 55804 2595, USA
    Aquat Toxicol 64:97-105. 2003
    ..01 mg/l, suggests that concerns for methoprene-mediated developmental toxicity to amphibians may be unwarranted...
  67. pmc Identification of metabolites of trenbolone acetate in androgenic runoff from a beef feedlot
    Elizabeth J Durhan
    Mid Continent Ecology Division, National Health and Environmental Effects Laboratory, U S Environmental Protection Agency, Duluth, Minnesota 55804, USA
    Environ Health Perspect 114:65-8. 2006
    ..Further work is needed to ascertain the degree to which synthetic androgens such as trenbolone contribute to androgenic activity of feedlot discharges...
  68. ncbi request reprint Assessment of the risk of solar ultraviolet radiation to amphibians. III. Prediction of impacts in selected northern midwestern wetlands
    Stephen A Diamond
    U S Environmental Protection Agency, National Health and Environmental Effects Research Laboratory, Mid Continent Ecology Division, Duluth, Minnesota 55804, USA
    Environ Sci Technol 36:2866-74. 2002
    ..However, continued reduction of ozone and other global climate change effects may increase UV doses in wetlands, suggesting that the risk of UV to amphibians should continue to be monitored and studied...
  69. ncbi request reprint Effects of the feedlot contaminant 17alpha-trenbolone on reproductive endocrinology of the fathead minnow
    Kathleen M Jensen
    U S Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Mid Continent Ecology Division, 6201 Congdon Boulevard, Duluth, Minnesota 55804, USA
    Environ Sci Technol 40:3112-7. 2006
    ..The present study demonstrates the importance of considering both alpha- and beta-trenbolone in assessing the potential ecological risk of androgens associated with beef feedlot discharges...
  70. doi request reprint Use of gene expression, biochemical and metabolite profiles to enhance exposure and effects assessment of the model androgen 17β-trenbolone in fish
    Drew R Ekman
    U S Environmental Protection Agency, Athens, Georgia, USA
    Environ Toxicol Chem 30:319-29. 2011
    ..Furthermore, TRB appeared to induce the hepatic metabolome of females to become more like that of males at both high test concentrations of TRB (472 ng/L) and more environmentally relevant levels (33 ng/L)...
  71. ncbi request reprint Investigating compensation and recovery of fathead minnow (Pimephales promelas) exposed to 17alpha-ethynylestradiol with metabolite profiling
    D R Ekman
    Ecosystems Research Division, U S EPA, 960 College Station Road, Athens, Georgia 30605, USA
    Environ Sci Technol 42:4188-94. 2008
    ..This revealed an apparent ability of the fish to compensate for the presence of EE2 later in the exposure, and to partially recover from its effects after the chemical was removed...
  72. ncbi request reprint Evaluation of bioaccumulation and photo-induced toxicity of fluoranthene in larval and adult life-stages of Chironomus tentans
    H E Bell
    Office of Science and Technology, Office of Water, US Environmental Protection Agency, MC4304T, Washington DC 20460, USA
    Arch Environ Contam Toxicol 47:297-303. 2004
    ..These studies indicated that both the burrowing behavior of the larvae and the elimination of fluoranthene in molted exuviae are protective mechanisms against photo-enhanced PAH toxicity in this species...
  73. ncbi request reprint Effects of low dissolved oxygen on organisms used in freshwater sediment toxicity tests
    Vincent R Mattson
    US Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Laboratory, Mid Continent Ecology Division, 6201 Congdon Boulevard, Duluth, MN 55804, USA
    Chemosphere 70:1840-4. 2008
    ..12 mg O(2)l(-1). The combination of these data with other literature data suggest that DO minima in current North American 10-d sediment test guidelines are reasonable...
  74. ncbi request reprint Effects of methoprene, its metabolites, and breakdown products on retinoid-activated pathways in transfected cell lines
    Patrick K Schoff
    U S Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Mid Continent Ecology Division, 6201 Congdon Boulevard, Duluth, Minnesota 55804, USA
    Environ Toxicol Chem 23:1305-10. 2004
    ....
  75. ncbi request reprint Xenoendocrine disrupters-tiered screening and testing: filling key data gaps
    L E Gray
    Endocrinology Branch, Reproductive Toxicology Division, National Health Environmental Effects Research Laboratory, ORD, US Environmental Protection Agency, MD 72, Research Triangle Park, NC 27111, USA
    Toxicology 181:371-82. 2002
    ..Tier 1 and 2 also should examine relevant mixtures of EDCs. Toxicants that induce malformations in AR-dependent tissues produce cumulative effects even when two chemicals act via different mechanisms of action...
  76. ncbi request reprint Amendment of sediments with a carbonaceous resin reduces bioavailability of polycyclic aromatic hydrocarbons
    C W West
    U S Environmental Protection Agency, 6201 Congdon Boulevard, Duluth, Minnesota 55804, USA
    Environ Toxicol Chem 20:1104-11. 2001
    ....
  77. ncbi request reprint Computational toxicology: framework, partnerships, and program development. September 29-30, 2003, Research Triangle Park, North Carolina
    Robert Kavlock
    Office of Research and Development, US Environmental Protection Agency, Washington, DC, USA
    Reprod Toxicol 19:265-80. 2005
  78. ncbi request reprint Evaluation of time-to-effects as a basis for quantifying the toxicity of contaminated sediments
    David L Defoe
    Mid Continent Ecology Division, National Health and Environmental Effects Research Laboratory, Office of Research and Development, US Environmental Protection Agency, 6201 Congdon Boulevard, Duluth, MN 55804, USA
    Chemosphere 51:1-5. 2003
    ..These studies demonstrate the feasibility of use of time-to-effects as a basis for quantifying the relative toxicity of contaminated sediments...
  79. ncbi request reprint Sensitivity of fetal rat testicular steroidogenesis to maternal prochloraz exposure and the underlying mechanism of inhibition
    Chad R Blystone
    Department of Environmental and Molecular Toxicology, NC State University, Raleigh, NC 27695, USA
    Toxicol Sci 97:512-9. 2007
    ..These results demonstrate that PCZ lowers testicular testosterone synthesis by inhibiting CYP17 activity which likely contributes to the induced malformations in androgen-dependent tissues of male offspring...
  80. ncbi request reprint Comparison of ELISAs for detecting vitellogenin in the fathead minnow (Pimephales promelas)
    Eve Mylchreest
    DuPont Haskell Laboratory for Health and Environmental Sciences, Elkton Road, PO Box 50, Newark, DE 19714, USA
    Comp Biochem Physiol C Toxicol Pharmacol 134:251-7. 2003
    ..The homologous fVTG assay is recommended to achieve accurate quantification of VTG levels in fathead minnows...
  81. ncbi request reprint Ecotoxicology--a multidisciplinary, problem-driven science
    Joop L M Hermens
    Urecht University, The Netherlands
    Environ Sci Technol 38:446A-447A. 2004
  82. ncbi request reprint Application of frog embryo teratogenesis assay-Xenopus to ecological risk assessment
    Robert A Hoke
    DuPont, Haskell Laboratory for Health and Environmental Sciences, Newark, Delaware 19714, USA
    Environ Toxicol Chem 24:2677-90. 2005
    ....
  83. pmc Fifteen years after "Wingspread"--environmental endocrine disrupters and human and wildlife health: where we are today and where we need to go
    Andrew K Hotchkiss
    USEPA NCSU Cooperative Training agreement CT826512010, Raleigh, North Carolina 27695, USA
    Toxicol Sci 105:235-59. 2008
    ....
  84. ncbi request reprint Development and validation of a 2,000-gene microarray for the fathead minnow (Pimephales promelas)
    Patrick Larkin
    EcoArray, 12085 Research Drive, Alachua, Florida 32615, USA
    Environ Toxicol Chem 26:1497-506. 2007
    ..Our studies indicate the feasibility and utility of microarrays as a basis for understanding biological responses to chemical exposure in a model ecotoxicology test species...
  85. ncbi request reprint What is normal? A characterization of the values and variability in reproductive endpoints of the fathead minnow, Pimephales promelas
    Karen H Watanabe
    Department of Environmental and Biomolecular Systems, OGI School of Science and Engineering, Oregon Health and Science University, 20000 N W Walker Rd, Beaverton, OR 97006, USA
    Comp Biochem Physiol C Toxicol Pharmacol 146:348-56. 2007
    ..Based on one of the largest datasets ever compiled for controlled studies, results presented herein provide a robust point of reference for the quantitative assessment of reproductive processes in the fathead minnow...
  86. pmc Screening and testing for endocrine disruption in fish-biomarkers as "signposts," not "traffic lights," in risk assessment
    Thomas H Hutchinson
    AstraZeneca Global Safety, Health and Environment, Brixham Environmental Laboratory, United Kingdom
    Environ Health Perspect 114:106-14. 2006
    ....
  87. doi request reprint Perturbation of gene expression and steroidogenesis with in vitro exposure of fathead minnow ovaries to ketoconazole
    Edward J Perkins
    Environmental Laboratory, US Army Engineer Research and Development Center, Vicksburg, MS 39180, USA
    Mar Environ Res 66:113-5. 2008
    ....