MARK ZYLKA

Summary

Affiliation: University of North Carolina
Country: USA

Publications

  1. pmc Recombinant mouse PAP has pH-dependent ectonucleotidase activity and acts through A(1)-adenosine receptors to mediate antinociception
    Nathaniel A Sowa
    Department of Cell and Molecular Physiology, UNC Neuroscience Center, University of North Carolina, Chapel Hill, North Carolina, USA
    PLoS ONE 4:e4248. 2009
  2. pmc Secretion and N-linked glycosylation are required for prostatic acid phosphatase catalytic and antinociceptive activity
    Julie K Hurt
    Department of Cell and Molecular Physiology, Neuroscience Center, University of North Carolina, Chapel Hill, North Carolina, United States of America
    PLoS ONE 7:e32741. 2012
  3. pmc Prostatic acid phosphatase is required for the antinociceptive effects of thiamine and benfotiamine
    Julie K Hurt
    Department of Cell and Molecular Physiology, UNC Neuroscience Center, University of North Carolina at Chapel Hill, United States of America
    PLoS ONE 7:e48562. 2012
  4. pmc CGRPα-expressing sensory neurons respond to stimuli that evoke sensations of pain and itch
    Eric S McCoy
    Department of Cell and Molecular Physiology, UNC Neuroscience Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States of America
    PLoS ONE 7:e36355. 2012
  5. pmc Prostatic acid phosphatase is expressed in peptidergic and nonpeptidergic nociceptive neurons of mice and rats
    Bonnie Taylor-Blake
    Department of Cell and Molecular Physiology, UNC Neuroscience Center, University of North Carolina, Chapel Hill, North Carolina, USA
    PLoS ONE 5:e8674. 2010
  6. pmc Biological constraints limit the use of rapamycin-inducible FKBP12-Inp54p for depleting PIP2 in dorsal root ganglia neurons
    Jaeda C Coutinho-Budd
    Curriculum in Neurobiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    J Negat Results Biomed 12:13. 2013
  7. pmc PAPupuncture has localized and long-lasting antinociceptive effects in mouse models of acute and chronic pain
    Julie K Hurt
    Department of Cell and Molecular Physiology, UNC Neuroscience Center, The University of North Carolina at Chapel Hill, CB 7545, Chapel Hill, NC 27599, USA
    Mol Pain 8:28. 2012
  8. pmc Recombinant ecto-5'-nucleotidase (CD73) has long lasting antinociceptive effects that are dependent on adenosine A1 receptor activation
    Nathaniel A Sowa
    Department of Cell and Molecular Physiology, UNC Neuroscience Center, University of North Carolina, CB 7545, Chapel Hill, North Carolina 27599, USA
    Mol Pain 6:20. 2010
  9. pmc Prostatic acid phosphatase is an ectonucleotidase and suppresses pain by generating adenosine
    Mark J Zylka
    Department of Cell and Molecular Physiology, UNC Neuroscience Center, University of North Carolina, CB 7545, Chapel Hill, NC 27599, USA
    Neuron 60:111-22. 2008
  10. pmc Pain-relieving prospects for adenosine receptors and ectonucleotidases
    Mark J Zylka
    Department of Cell and Molecular Physiology, UNC Neuroscience Center, University of North Carolina, Chapel Hill, NC 27599, USA
    Trends Mol Med 17:188-96. 2011

Research Grants

Collaborators

  • Nathaniel A Sowa
  • Julie K Hurt
  • Kunjumon I Vadakkan
  • Bonnie Taylor-Blake
  • Hong Wang
  • Jaeda C Coutinho-Budd
  • Eric S McCoy
  • Sarah E Street
  • Luke Campagnola
  • Joseph E Rittiner
  • Brendan J Fitzpatrick
  • Samuel B Snider
  • Thomas S Guillot
  • Paul L Walsh
  • Pirkko Vihko
  • R Mark Wightman

Detail Information

Publications16

  1. pmc Recombinant mouse PAP has pH-dependent ectonucleotidase activity and acts through A(1)-adenosine receptors to mediate antinociception
    Nathaniel A Sowa
    Department of Cell and Molecular Physiology, UNC Neuroscience Center, University of North Carolina, Chapel Hill, North Carolina, USA
    PLoS ONE 4:e4248. 2009
    ..Lastly, our studies demonstrate that recombinant PAP protein can be used to treat chronic pain in animal models...
  2. pmc Secretion and N-linked glycosylation are required for prostatic acid phosphatase catalytic and antinociceptive activity
    Julie K Hurt
    Department of Cell and Molecular Physiology, Neuroscience Center, University of North Carolina, Chapel Hill, North Carolina, United States of America
    PLoS ONE 7:e32741. 2012
    ..Moreover, our findings indicate that recombinant hPAP can be produced in Pichia--a yeast strain that is used to generate biologics for therapeutic purposes...
  3. pmc Prostatic acid phosphatase is required for the antinociceptive effects of thiamine and benfotiamine
    Julie K Hurt
    Department of Cell and Molecular Physiology, UNC Neuroscience Center, University of North Carolina at Chapel Hill, United States of America
    PLoS ONE 7:e48562. 2012
    ..Taken together, our study highlights an obligatory role for PAP in the antinociceptive effects of thiamine and phosphorylated thiamine analogs, and suggests a novel phosphatase-independent function for PAP...
  4. pmc CGRPα-expressing sensory neurons respond to stimuli that evoke sensations of pain and itch
    Eric S McCoy
    Department of Cell and Molecular Physiology, UNC Neuroscience Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States of America
    PLoS ONE 7:e36355. 2012
    ..In future studies, it should be possible to conditionally ablate CGRPα-expressing neurons to evaluate sensory and non-sensory functions for these neurons...
  5. pmc Prostatic acid phosphatase is expressed in peptidergic and nonpeptidergic nociceptive neurons of mice and rats
    Bonnie Taylor-Blake
    Department of Cell and Molecular Physiology, UNC Neuroscience Center, University of North Carolina, Chapel Hill, North Carolina, USA
    PLoS ONE 5:e8674. 2010
    ..Collectively, our studies highlight similarities and differences in PAP localization within nociceptive neurons of mice and rats...
  6. pmc Biological constraints limit the use of rapamycin-inducible FKBP12-Inp54p for depleting PIP2 in dorsal root ganglia neurons
    Jaeda C Coutinho-Budd
    Curriculum in Neurobiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    J Negat Results Biomed 12:13. 2013
    ..Here, we sought to adapt a rapamycin-inducible phosphatidylinositol 4,5-bisphosphate (PIP2)-specific phosphatase (Inp54p) system to deplete PIP2 in nociceptive dorsal root ganglia (DRG) neurons...
  7. pmc PAPupuncture has localized and long-lasting antinociceptive effects in mouse models of acute and chronic pain
    Julie K Hurt
    Department of Cell and Molecular Physiology, UNC Neuroscience Center, The University of North Carolina at Chapel Hill, CB 7545, Chapel Hill, NC 27599, USA
    Mol Pain 8:28. 2012
    ....
  8. pmc Recombinant ecto-5'-nucleotidase (CD73) has long lasting antinociceptive effects that are dependent on adenosine A1 receptor activation
    Nathaniel A Sowa
    Department of Cell and Molecular Physiology, UNC Neuroscience Center, University of North Carolina, CB 7545, Chapel Hill, North Carolina 27599, USA
    Mol Pain 6:20. 2010
    ..Since adenosine has antinociceptive effects in rodents and humans, we hypothesized that NT5E, an enzyme that generates adenosine, might also have antinociceptive effects in vivo...
  9. pmc Prostatic acid phosphatase is an ectonucleotidase and suppresses pain by generating adenosine
    Mark J Zylka
    Department of Cell and Molecular Physiology, UNC Neuroscience Center, University of North Carolina, CB 7545, Chapel Hill, NC 27599, USA
    Neuron 60:111-22. 2008
    ..Our studies reveal molecular and physiological functions for PAP in purine nucleotide metabolism and nociception and suggest a novel use for PAP in the treatment of chronic pain...
  10. pmc Pain-relieving prospects for adenosine receptors and ectonucleotidases
    Mark J Zylka
    Department of Cell and Molecular Physiology, UNC Neuroscience Center, University of North Carolina, Chapel Hill, NC 27599, USA
    Trends Mol Med 17:188-96. 2011
    ..Collectively, these studies suggest the possibility of treating chronic pain in humans by targeting specific adenosine receptor subtypes in anatomically defined regions with agonists or with ectonucleotidases that generate adenosine...
  11. pmc PAP and NT5E inhibit nociceptive neurotransmission by rapidly hydrolyzing nucleotides to adenosine
    Sarah E Street
    Department of Cell and Molecular Physiology, University of North Carolina, CB 7545, Chapel Hill, North Carolina 27599, USA
    Mol Pain 7:80. 2011
    ....
  12. pmc Ecto-5'-nucleotidase (CD73) inhibits nociception by hydrolyzing AMP to adenosine in nociceptive circuits
    Nathaniel A Sowa
    Department of Cell and Molecular Physiology, UNC Neuroscience Center and Curriculum in Neurobiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    J Neurosci 30:2235-44. 2010
    ..Moreover, our data suggest NT5E is well localized to regulate nucleotide signaling between skin cells and sensory axons...
  13. pmc Prostatic acid phosphatase reduces thermal sensitivity and chronic pain sensitization by depleting phosphatidylinositol 4,5-bisphosphate
    Nathaniel A Sowa
    Department of Cell and Molecular Physiology, University of North Carolina Neuroscience Center, Chapel Hill, North Carolina 27599, USA
    J Neurosci 30:10282-93. 2010
    ..This tone regulates how effectively acute nociceptive insults promote the transition to chronic pain...
  14. pmc Mrgprd-expressing polymodal nociceptive neurons innervate most known classes of substantia gelatinosa neurons
    Hong Wang
    Department of Cell and Molecular Physiology, University of North Carolina Neuroscience Center, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    J Neurosci 29:13202-9. 2009
    ..Our findings set broad constraints on the types of spinal neurons that process afferent input from Mrgprd(+) polymodal nociceptors...
  15. doi request reprint Fiber-coupled light-emitting diode for localized photostimulation of neurons expressing channelrhodopsin-2
    Luke Campagnola
    Department of Cell and Molecular Physiology, 5109A NRB, CB 7545, 115 Mason Farm Road, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7545, United States
    J Neurosci Methods 169:27-33. 2008
    ..This scalable fiber-coupled LED system can be used to deliver light independent of the microscope objective and could, in principle, deliver light in parallel to multiple brain regions or to multiple genetically engineered animals...
  16. ncbi request reprint Nonpeptidergic circuits feel your pain
    Mark J Zylka
    Department of Cell and Molecular Physiology, UNC Neuroscience Center, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599, USA
    Neuron 47:771-2. 2005
    ..They found that these neurons give rise to a specialized multisynaptic circuit that links pain signals in the periphery to limbic/affective regions of the brain...

Research Grants6

  1. Harnessing ectonucleotidases to treat chronic pain
    MARK JOHN ZYLKA; Fiscal Year: 2010
    ..In addition, these studies have the potential to transform how we treat pain in millions of patients with fewer side effects. ..
  2. Biochemical Modulation of Nociceptive Circuits
    MARK ZYLKA; Fiscal Year: 2007
    ..Finally, we will test the hypothesis that this phosphatase modulates neuropathic pain behaviors in wild-type and phosphatase knockout mice. ..
  3. Biochemical Modulation of Nociceptive Circuits
    MARK ZYLKA; Fiscal Year: 2009
    ..Finally, we will test the hypothesis that this phosphatase modulates neuropathic pain behaviors in wild-type and phosphatase knockout mice. ..
  4. Harnessing ectonucleotidases to treat chronic pain
    MARK ZYLKA; Fiscal Year: 2009
    ..In addition, these studies have the potential to transform how we treat pain in millions of patients with fewer side effects. ..
  5. Biochemical Modulation of Nociceptive Circuits
    MARK JOHN ZYLKA; Fiscal Year: 2010
    ..Finally, we will test the hypothesis that this phosphatase modulates neuropathic pain behaviors in wild-type and phosphatase knockout mice. ..