Research Topics
Genomes and Genes
| Michael ZuscikSummaryAffiliation: University of Rochester Country: USA Publications
Research Grants
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Detail Information
Publications
CREB Cooperates with BMP-stimulated Smad signaling to enhance transcription of the Smad6 promoterAndreia M Ionescu
Center for Musculoskeletal Research, University of Rochester, School of Medicine and Dentistry, Rochester, New York, USA
J Cell Physiol 198:428-40. 2004....- Parathyroid hormone-related peptide (PTHrP) inhibits Runx2 expression through the PKA signaling pathwayTian Fang Li
Department of Orthopaedics, The Center for Musculoskeletal Research, University of Rochester, Rochester, NY 14642, USA
Exp Cell Res 299:128-36. 2004..Overall, our results demonstrate that PTHrP downregulates Runx2 expression primarily through the PKA signaling pathway... 
High-fat diet accelerates progression of osteoarthritis after meniscal/ligamentous injuryRobert A Mooney
Department of Pathology and Laboratory Medicine, Box 626, University of Rochester Medical Center, 601 Elmwood Avenue, Rochester, NY 14642, USA
Arthritis Res Ther 13:R198. 2011..This study was undertaken to establish a causal relation between the HF diet and accelerated OA progression in a mouse model and to determine the relative roles of weight gain and metabolic dysregulation in this progression...- Growth plate chondrocyte maturation is regulated by basal intracellular calciumMichael J Zuscik
Center for Musculoskeletal Research, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA
Exp Cell Res 276:310-9. 2002..These findings underscore the importance of cellular Ca(2+) in GPC function and suggest that PTHrP action in the growth plate is at least partially regulated by changes in basal [Ca(2+)](i)... - 5-azacytidine alters TGF-beta and BMP signaling and induces maturation in articular chondrocytesMichael J Zuscik
Center for Musculoskeletal Research, Department of Orthopaedics, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA
J Cell Biochem 92:316-31. 2004.... - Parathyroid hormone-related peptide regulation of chick tibial growth plate chondrocyte maturation requires protein kinase AMichael J Zuscik
Department of Orthopaedics, School of Medicine and Dentistry, University of Rochester, NY 14642, USA
J Orthop Res 20:1079-90. 2002..These data demonstrate that although activation of PKC or Ca2+ signals is not required, the cylic AMP-dependent A kinase is required for PTHrP to regulate key hallmarks of GPC phenotype... - Activation of beta-catenin signaling in articular chondrocytes leads to osteoarthritis-like phenotype in adult beta-catenin conditional activation miceMei Zhu
Department of Orthopaedics, Center for Musculoskeletal Research, University of Rochester School of Medicine, Rochester, New York 14642, USA
J Bone Miner Res 24:12-21. 2009..This study provides direct and definitive evidence about the role of beta-catenin in the development of OA... - Murine and chicken chondrocytes regulate osteoclastogenesis by producing RANKL in response to BMP2Michihiko Usui
Department of Pathology and Laboratory Medicine, University of Rochester Medical Center, Rochester, New York 14642, USA
J Bone Miner Res 23:314-25. 2008..We report that hypertrophic chondrocytes induce osteoclast formation through RANKL production stimulated by BMP2 and Runx2/Smad1 and thus they may regulate resorption of calcified matrix by osteoclasts at growth plates... - Wnt-mediated regulation of chondrocyte maturation: modulation by TGF-betaYufeng Dong
Center for Musculoskeletal Research, University of Rochester Medical Center, Rochester, New York 14642, USA
J Cell Biochem 95:1057-68. 2005..Moreover, this pathway is modulated by members of the TGF-beta family and demonstrates the highly integrated nature of signals controlling endochondral ossification... 
Lead induces an osteoarthritis-like phenotype in articular chondrocytes through disruption of TGF-β signalingJonathan D Holz
Department of Orthopaedics, University of Rochester School of Medicine and Dentristry, Rochester, NY 14642, USA
J Orthop Res 30:1760-6. 2012..These changes closely parallel those seen in osteoarthritis. Over time this phenotypic shift could compromise maintenance of the joint matrix...- Retinoic acid stimulates chondrocyte differentiation and enhances bone morphogenetic protein effects through induction of Smad1 and Smad5Xuefeng Li
Center for Musculoskeletal Research, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA
Endocrinology 144:2514-23. 2003..These findings demonstrate that RA induces the expression of BMP-signaling molecules and enhances BMP effects in chondrocytes... - Inhibition of beta-catenin signaling in articular chondrocytes results in articular cartilage destructionMei Zhu
University of Rochester, Rochester, New York 14642, USA
Arthritis Rheum 58:2053-64. 2008..To determine the role of beta-catenin signaling in articular chondrocyte function, we generated Col2a1-ICAT-transgenic mice to inhibit beta-catenin signaling in chondrocytes... - Runx2/Cbfa1 stimulation by retinoic acid is potentiated by BMP2 signaling through interaction with Smad1 on the collagen X promoter in chondrocytesM Hicham Drissi
The Center for Musculoskeletal Research, University of Rochester, Rochester, New York 14642, USA
J Cell Biochem 90:1287-98. 2003..Factors or signals that alter either of these transcription factors regulate the rate of chondrocyte differentiation... - Lead alters parathyroid hormone-related peptide and transforming growth factor-beta1 effects and AP-1 and NF-kappaB signaling in chondrocytesMichael J Zuscik
Center for Muscluskeletal Research, School of Medicine and Dentistry, University of Rochester, NY 14642, USA
J Orthop Res 20:811-8. 2002.... - PGE2 inhibits chondrocyte differentiation through PKA and PKC signalingTian-Fang Li
Center for Musculoskeletal Research, School of Medicine and Dentistry, University of Rochester, Rochester, NY 14642, USA
Exp Cell Res 300:159-69. 2004..These observations demonstrate potent prostaglandin regulatory effects on chondrocyte maturation and show a role for both PKA and PKC signaling in PGE2 regulatory events... - Axin1 and Axin2 are regulated by TGF- and mediate cross-talk between TGF- and Wnt signaling pathwaysDebbie Y Dao
Department of Orthopaedics, Center for Musculoskeletal Research University of Rochester School of Medicine, Rochester, NY 14642, USA
Ann N Y Acad Sci 1116:82-99. 2007.... - Primary murine limb bud mesenchymal cells in long-term culture complete chondrocyte differentiation: TGF-beta delays hypertrophy and PGE2 inhibits terminal differentiationXinping Zhang
Department of Orthopaedics, Center for Musculoskeletal Research, University of Rochester, Rochester, NY 14642, USA
Bone 34:809-17. 2004.... - Runx3/AML2/Cbfa3 regulates early and late chondrocyte differentiationDo Y Soung
Center for Musculoskeletal Research, University of Rochester, Rochester, New York 14642, USA
J Bone Miner Res 22:1260-70. 2007..We found that Runx3 is essential for mediating the early stage of endochondral ossification through cooperation with other Runx family members... - ALK2 functions as a BMP type I receptor and induces Indian hedgehog in chondrocytes during skeletal developmentDonghui Zhang
Center for Musculoskeletal Research, University of Rochester, Rochester, New York 14642, USA
J Bone Miner Res 18:1593-604. 2003..The findings suggest that BMP signaling modulates the Ihh/PTHrP signaling pathway that regulates the rate of chondrocyte differentiation... - Beta-catenin, cartilage, and osteoarthritisQiuqian Wu
Department of Orthopaedics and Rehabilitation, Center for Musculoskeletal Research, University of Rochester School of Medicine and Dentistry, Rochester, New York, USA
Ann N Y Acad Sci 1192:344-50. 2010..These findings indicate that beta-catenin plays a central role in articular cartilage function and that activation of beta-catenin signaling may represent a pathologic mechanism for OA development... - Transforming growth factor-beta1 induced alteration of skeletal morphogenesis in vivoCristin M Ferguson
Department of Orthopaedics, Center for Musculoskeletal Research, University of Rochester School of Medicine and Dentistry, 601 Elmwood Ave, Box 665, Rochester, NY 14642, USA
J Orthop Res 22:687-96. 2004..The findings confirm a critical role for TGF-beta during skeletal development. A more complete understanding of the role of TGF-beta and its down-stream signals will lead to improved understanding and treatment of cartilage diseases... - Prostaglandin E2 inhibits BMP signaling and delays chondrocyte maturationChristine A Clark
Center for Musculoskeletal Research, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA
J Orthop Res 27:785-92. 2009..18 cells and in primary murine sternal chondrocytes. Altogether, the findings show that PGE2 regulates chondrocyte maturation in part by targeting BMP/Smad signaling and suggest an important role for PGE2 in endochondral bone formation... - Transforming growth factor-beta stimulates cyclin D1 expression through activation of beta-catenin signaling in chondrocytesTian Fang Li
Department of Orthopaedics, Center for Musculoskeletal Research, University of Rochester, New York 14642, USA
J Biol Chem 281:21296-304. 2006..These results demonstrate that TGF-beta stimulates cyclin D(1) expression at least in part through activation of beta-catenin signaling... 
Lead exposure inhibits fracture healing and is associated with increased chondrogenesis, delay in cartilage mineralization, and a decrease in osteoprogenitor frequencyJonathan J Carmouche
Center for Musculoskeletal Research, University of Rochester Medical Center, University of Rochester, School of Medicine and Dentistry, Rochester, New York 14642, USA
Environ Health Perspect 113:749-55. 2005..We conclude that Pb delays fracture healing at environmentally relevant doses and induces fibrous nonunions at higher doses by inhibiting the progression of endochondral ossification...- Induction of an osteoarthritis-like phenotype and degradation of phosphorylated Smad3 by Smurf2 in transgenic miceQiuqian Wu
Department of Orthopaedics, University of Rochester Medical Center, Rochester, New York 14642, USA
Arthritis Rheum 58:3132-44. 2008..To determine whether Smurf2, an E3 ubiquitin ligase known to inhibit transforming growth factor beta (TGFbeta) signaling, is expressed in human osteoarthritic (OA) cartilage and can initiate OA in mice... - ATF-2 cooperates with Smad3 to mediate TGF-beta effects on chondrocyte maturationAndreia M Ionescu
Center for Musculoskeletal Research, School of Medicine and Dentistry, University of Rochester, Rochester, NY 14642, USA
Exp Cell Res 288:198-207. 2003..Thus, ATF-2 regulates chondrocyte maturation as a direct target of TGF-beta signaling while CREB regulates differentiation by targeting genes independent of the individual signaling effects of TGF-beta or BMP-2... - Smad3-deficient chondrocytes have enhanced BMP signaling and accelerated differentiationTian Fang Li
Center for Musculoskeletal Research, University of Rochester School of Medicine and Dentistry, Rochester, New York, USA
J Bone Miner Res 21:4-16. 2006..Smad2 or Smad3 overexpression and BMP blockade abrogate accelerated maturation in Smad3-/- chondrocytes. BMP signaling is increased in TGF-beta deficiency and is required for accelerated chondrocyte maturation... - Aberrant hypertrophy in Smad3-deficient murine chondrocytes is rescued by restoring transforming growth factor beta-activated kinase 1/activating transcription factor 2 signaling: a potential clinical implication for osteoarthritisTian Fang Li
University of Rochester, Rochester, New York 14642, USA
Arthritis Rheum 62:2359-69. 2010.... - Transcriptional regulation of chondrocyte maturation: potential involvement of transcription factors in OA pathogenesisHicham Drissi
The Center for Musculoskeletal Research, University of Rochester, Rochester, NY 14642, United States
Mol Aspects Med 26:169-79. 2005..Thus, unraveling the molecular interplay between various factors that regulate chondrocyte maturation during OA occurrence and progression is the main focus of ongoing efforts... - Smurf2 induces degradation of GSK-3beta and upregulates beta-catenin in chondrocytes: a potential mechanism for Smurf2-induced degeneration of articular cartilageQiuqian Wu
Center for Musculoskeletal Research, University of Rochester Medical Center, 601 Elmwood Avenue, Rochester, NY 14642, USA
Exp Cell Res 315:2386-98. 2009.... - Regulation of embryonic endochondral ossification by Smurf2Qiuqian Wu
Department of Orthopaedics, Center for Musculoskeletal Research, University of Rochester Medical Center, 601 Elmwood Avenue, Box 665, Rochester, New York 14642, USA
J Orthop Res 26:704-12. 2008.... - Overexpression of Smurf2 stimulates endochondral ossification through upregulation of beta-cateninQiuqian Wu
Department of Orthopaedics, Center for Musculoskeletal Research, University of Rochester Medical Center, 601 Elmwood Avenue, Box 665, Rochester, New York 14642, USA
J Bone Miner Res 23:552-63. 2008..The mechanism underlying Smurf2-mediated morphological changes during embryonic development may provide new mechanistic insights and potential targets for prevention and treatment of human osteoarthritis... - Smad7 mediates inhibition of Saos2 osteosarcoma cell differentiation by NFkappaBRoman A Eliseev
Center for Musculoskeletal Research, University of Rochester Medical Center, Rochester, NY 14642, USA
Exp Cell Res 312:40-50. 2006..We therefore conclude that in osteosarcoma Saos2 cells, NFkappaB represses BMP/Smad signaling and BMP2-induced differentiation through Smad7... - RBPjkappa-dependent Notch signaling regulates mesenchymal progenitor cell proliferation and differentiation during skeletal developmentYufeng Dong
Department of Orthopaedics and Rehabilitation, Center for Musculoskeletal Research, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA
Development 137:1461-71. 2010..Finally, Hes1 was identified as an RBPjkappa-dependent Notch target gene important for MPC maintenance and the suppression of in vitro chondrogenesis... - Smad6 is induced by BMP-2 and modulates chondrocyte differentiationXeufeng Li
Center for Musculoskeletal Research, Department of Orthopaedics, University of Rochester Medical Center, 601 Elmwood Avenue, Box 665, Rochester, NY 14642, USA
J Orthop Res 21:908-13. 2003..Additional studies are required to determine the extent to which this pathway participates in pathologic processes involving cartilage... - Lead induces chondrogenesis and alters transforming growth factor-beta and bone morphogenetic protein signaling in mesenchymal cell populationsMichael J Zuscik
Center for Musculoskeletal Research, University of Rochester School of Medicine and Dentistry, Rochester, New York, USA
Environ Health Perspect 115:1276-82. 2007..Because chondrogenesis is a seminal step during skeletal development, elucidating the impact of Pb on this process is the first step toward understanding the mechanism of Pb toxicity in the skeleton... - A phage display technique identifies a novel regulator of cell differentiationTzong Jen Sheu
Department of Orthopaedics, Center for Musculoskeletal Research, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA
J Biol Chem 278:438-43. 2003..We hypothesize that binding to TRAP may be one mechanism by which the full osteoblast phenotype is expressed during the process of bone remodeling... - Regulation of chondrogenesis and chondrocyte differentiation by stressMichael J Zuscik
Center for Musculoskeletal Research, University of Rochester Medical Center, Rochester, New York 14642, USA
J Clin Invest 118:429-38. 2008..A summary of the influence of the most well-understood normal and pathologic stressors on the differentiation program is also presented... - GPCR kinase 2 interacting protein 1 (GIT1) regulates osteoclast function and bone massPrashanthi Menon
Department of Medicine and Aab Cardiovascular Research Institute, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA
J Cell Physiol 225:777-85. 2010..These data show that GIT1 is a key regulator of bone mass in vivo by regulating osteoclast function and suggest GIT1 as a potential target for osteoporosis therapy... - Environmental agents affect skeletal growth and developmentJonathan D Holz
Department of Orthopaedics and the Center for Musculoskeletal Research, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA
Birth Defects Res C Embryo Today 81:41-50. 2007..Research in this area will identify strategies that may be used to help prevent musculoskeletal diseases due to toxicant exposure... - Differential regulation of EP receptor isoforms during chondrogenesis and chondrocyte maturationChristine A Clark
Center for Musculoskeletal Research, University of Rochester, School of Medicine and Dentistry, Rochester, NY 14642, USA
Biochem Biophys Res Commun 328:764-76. 2005.... - Increased radiation-induced apoptosis of Saos2 cells via inhibition of NFkappaB: a role for c-Jun N-terminal kinaseRoman A Eliseev
Center for Musculoskeletal Research, University of Rochester Medical Center, Rochester, New York 14642, USA
J Cell Biochem 96:1262-73. 2005..Our study brings new insight into the mechanisms underlying radiation-induced apoptosis of osteosarcoma, and may lead to development of new therapeutic strategies against osteosarcoma... - Reduced COX-2 expression in aged mice is associated with impaired fracture healingAmish A Naik
The Center for Musculoskeletal Research, University of Rochester, Rochester, New York 14642, USA
J Bone Miner Res 24:251-64. 2009..The findings suggest that COX-2/EP4 agonists may compensate for deficient molecular signals that result in the reduced fracture healing associated with aging... - Gene expression profiling of alpha(1b)-adrenergic receptor-induced cardiac hypertrophy by oligonucleotide arraysJune Yun
The Department of Molecular Cardiology NB50, The Lerner Research Institute, The Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, OH 44195, USA
Cardiovasc Res 57:443-55. 2003..Since many of these altered gene expressions have not been linked to heart failure models, our findings may provide a novel insight into the particular role that the alpha(1B)AR plays in its overall progression or regression... - Mice expressing the alpha(1B)-adrenergic receptor induces a synucleinopathy with excessive tyrosine nitration but decreased phosphorylationRobert Papay
Department of Molecular Cardiology, The Lerner Research Institute, The Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, OH 44195, USA
J Neurochem 83:623-34. 2002..We conclude that overexpression of the alpha(1B)-AR can cause a synucleinopathy similar to other parkinsonian syndromes... - Altered negative regulation of transforming growth factor beta signaling in scleroderma: potential involvement of SMURF2 in diseaseMichael J Zuscik
Arthritis Rheum 48:1779-80. 2003
Research Grants
- Mechanism of Nicotine Action in the Healing SkeletonMichael Zuscik; Fiscal Year: 2007....
- Mechanism of Nicotine Action in the Healing SkeletonMichael Zuscik; Fiscal Year: 2009....