Michael Zuscik

Summary

Affiliation: University of Rochester
Country: USA

Publications

  1. ncbi request reprint CREB Cooperates with BMP-stimulated Smad signaling to enhance transcription of the Smad6 promoter
    Andreia M Ionescu
    Center for Musculoskeletal Research, University of Rochester, School of Medicine and Dentistry, Rochester, New York, USA
    J Cell Physiol 198:428-40. 2004
  2. ncbi request reprint Parathyroid hormone-related peptide (PTHrP) inhibits Runx2 expression through the PKA signaling pathway
    Tian Fang Li
    Department of Orthopaedics, The Center for Musculoskeletal Research, University of Rochester, Rochester, NY 14642, USA
    Exp Cell Res 299:128-36. 2004
  3. pmc High-fat diet accelerates progression of osteoarthritis after meniscal/ligamentous injury
    Robert A Mooney
    Department of Pathology and Laboratory Medicine, Box 626, University of Rochester Medical Center, 601 Elmwood Avenue, Rochester, NY 14642, USA
    Arthritis Res Ther 13:R198. 2011
  4. ncbi request reprint Growth plate chondrocyte maturation is regulated by basal intracellular calcium
    Michael J Zuscik
    Center for Musculoskeletal Research, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA
    Exp Cell Res 276:310-9. 2002
  5. ncbi request reprint 5-azacytidine alters TGF-beta and BMP signaling and induces maturation in articular chondrocytes
    Michael J Zuscik
    Center for Musculoskeletal Research, Department of Orthopaedics, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA
    J Cell Biochem 92:316-31. 2004
  6. ncbi request reprint Parathyroid hormone-related peptide regulation of chick tibial growth plate chondrocyte maturation requires protein kinase A
    Michael J Zuscik
    Department of Orthopaedics, School of Medicine and Dentistry, University of Rochester, NY 14642, USA
    J Orthop Res 20:1079-90. 2002
  7. pmc Activation of beta-catenin signaling in articular chondrocytes leads to osteoarthritis-like phenotype in adult beta-catenin conditional activation mice
    Mei Zhu
    Department of Orthopaedics, Center for Musculoskeletal Research, University of Rochester School of Medicine, Rochester, New York 14642, USA
    J Bone Miner Res 24:12-21. 2009
  8. pmc Murine and chicken chondrocytes regulate osteoclastogenesis by producing RANKL in response to BMP2
    Michihiko Usui
    Department of Pathology and Laboratory Medicine, University of Rochester Medical Center, Rochester, New York 14642, USA
    J Bone Miner Res 23:314-25. 2008
  9. pmc Wnt-mediated regulation of chondrocyte maturation: modulation by TGF-beta
    Yufeng Dong
    Center for Musculoskeletal Research, University of Rochester Medical Center, Rochester, New York 14642, USA
    J Cell Biochem 95:1057-68. 2005
  10. doi request reprint Lead induces an osteoarthritis-like phenotype in articular chondrocytes through disruption of TGF-β signaling
    Jonathan D Holz
    Department of Orthopaedics, University of Rochester School of Medicine and Dentristry, Rochester, NY 14642, USA
    J Orthop Res 30:1760-6. 2012

Research Grants

Collaborators

Detail Information

Publications46

  1. ncbi request reprint CREB Cooperates with BMP-stimulated Smad signaling to enhance transcription of the Smad6 promoter
    Andreia M Ionescu
    Center for Musculoskeletal Research, University of Rochester, School of Medicine and Dentistry, Rochester, New York, USA
    J Cell Physiol 198:428-40. 2004
    ....
  2. ncbi request reprint Parathyroid hormone-related peptide (PTHrP) inhibits Runx2 expression through the PKA signaling pathway
    Tian Fang Li
    Department of Orthopaedics, The Center for Musculoskeletal Research, University of Rochester, Rochester, NY 14642, USA
    Exp Cell Res 299:128-36. 2004
    ..Overall, our results demonstrate that PTHrP downregulates Runx2 expression primarily through the PKA signaling pathway...
  3. pmc High-fat diet accelerates progression of osteoarthritis after meniscal/ligamentous injury
    Robert A Mooney
    Department of Pathology and Laboratory Medicine, Box 626, University of Rochester Medical Center, 601 Elmwood Avenue, Rochester, NY 14642, USA
    Arthritis Res Ther 13:R198. 2011
    ..This study was undertaken to establish a causal relation between the HF diet and accelerated OA progression in a mouse model and to determine the relative roles of weight gain and metabolic dysregulation in this progression...
  4. ncbi request reprint Growth plate chondrocyte maturation is regulated by basal intracellular calcium
    Michael J Zuscik
    Center for Musculoskeletal Research, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA
    Exp Cell Res 276:310-9. 2002
    ..These findings underscore the importance of cellular Ca(2+) in GPC function and suggest that PTHrP action in the growth plate is at least partially regulated by changes in basal [Ca(2+)](i)...
  5. ncbi request reprint 5-azacytidine alters TGF-beta and BMP signaling and induces maturation in articular chondrocytes
    Michael J Zuscik
    Center for Musculoskeletal Research, Department of Orthopaedics, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA
    J Cell Biochem 92:316-31. 2004
    ....
  6. ncbi request reprint Parathyroid hormone-related peptide regulation of chick tibial growth plate chondrocyte maturation requires protein kinase A
    Michael J Zuscik
    Department of Orthopaedics, School of Medicine and Dentistry, University of Rochester, NY 14642, USA
    J Orthop Res 20:1079-90. 2002
    ..These data demonstrate that although activation of PKC or Ca2+ signals is not required, the cylic AMP-dependent A kinase is required for PTHrP to regulate key hallmarks of GPC phenotype...
  7. pmc Activation of beta-catenin signaling in articular chondrocytes leads to osteoarthritis-like phenotype in adult beta-catenin conditional activation mice
    Mei Zhu
    Department of Orthopaedics, Center for Musculoskeletal Research, University of Rochester School of Medicine, Rochester, New York 14642, USA
    J Bone Miner Res 24:12-21. 2009
    ..This study provides direct and definitive evidence about the role of beta-catenin in the development of OA...
  8. pmc Murine and chicken chondrocytes regulate osteoclastogenesis by producing RANKL in response to BMP2
    Michihiko Usui
    Department of Pathology and Laboratory Medicine, University of Rochester Medical Center, Rochester, New York 14642, USA
    J Bone Miner Res 23:314-25. 2008
    ..We report that hypertrophic chondrocytes induce osteoclast formation through RANKL production stimulated by BMP2 and Runx2/Smad1 and thus they may regulate resorption of calcified matrix by osteoclasts at growth plates...
  9. pmc Wnt-mediated regulation of chondrocyte maturation: modulation by TGF-beta
    Yufeng Dong
    Center for Musculoskeletal Research, University of Rochester Medical Center, Rochester, New York 14642, USA
    J Cell Biochem 95:1057-68. 2005
    ..Moreover, this pathway is modulated by members of the TGF-beta family and demonstrates the highly integrated nature of signals controlling endochondral ossification...
  10. doi request reprint Lead induces an osteoarthritis-like phenotype in articular chondrocytes through disruption of TGF-β signaling
    Jonathan D Holz
    Department of Orthopaedics, University of Rochester School of Medicine and Dentristry, Rochester, NY 14642, USA
    J Orthop Res 30:1760-6. 2012
    ..These changes closely parallel those seen in osteoarthritis. Over time this phenotypic shift could compromise maintenance of the joint matrix...
  11. ncbi request reprint Retinoic acid stimulates chondrocyte differentiation and enhances bone morphogenetic protein effects through induction of Smad1 and Smad5
    Xuefeng Li
    Center for Musculoskeletal Research, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA
    Endocrinology 144:2514-23. 2003
    ..These findings demonstrate that RA induces the expression of BMP-signaling molecules and enhances BMP effects in chondrocytes...
  12. pmc Inhibition of beta-catenin signaling in articular chondrocytes results in articular cartilage destruction
    Mei Zhu
    University of Rochester, Rochester, New York 14642, USA
    Arthritis Rheum 58:2053-64. 2008
    ..To determine the role of beta-catenin signaling in articular chondrocyte function, we generated Col2a1-ICAT-transgenic mice to inhibit beta-catenin signaling in chondrocytes...
  13. ncbi request reprint Runx2/Cbfa1 stimulation by retinoic acid is potentiated by BMP2 signaling through interaction with Smad1 on the collagen X promoter in chondrocytes
    M Hicham Drissi
    The Center for Musculoskeletal Research, University of Rochester, Rochester, New York 14642, USA
    J Cell Biochem 90:1287-98. 2003
    ..Factors or signals that alter either of these transcription factors regulate the rate of chondrocyte differentiation...
  14. ncbi request reprint Lead alters parathyroid hormone-related peptide and transforming growth factor-beta1 effects and AP-1 and NF-kappaB signaling in chondrocytes
    Michael J Zuscik
    Center for Muscluskeletal Research, School of Medicine and Dentistry, University of Rochester, NY 14642, USA
    J Orthop Res 20:811-8. 2002
    ....
  15. ncbi request reprint PGE2 inhibits chondrocyte differentiation through PKA and PKC signaling
    Tian Fang Li
    Center for Musculoskeletal Research, School of Medicine and Dentistry, University of Rochester, Rochester, NY 14642, USA
    Exp Cell Res 300:159-69. 2004
    ..These observations demonstrate potent prostaglandin regulatory effects on chondrocyte maturation and show a role for both PKA and PKC signaling in PGE2 regulatory events...
  16. pmc Axin1 and Axin2 are regulated by TGF- and mediate cross-talk between TGF- and Wnt signaling pathways
    Debbie Y Dao
    Department of Orthopaedics, Center for Musculoskeletal Research University of Rochester School of Medicine, Rochester, NY 14642, USA
    Ann N Y Acad Sci 1116:82-99. 2007
    ....
  17. ncbi request reprint Primary murine limb bud mesenchymal cells in long-term culture complete chondrocyte differentiation: TGF-beta delays hypertrophy and PGE2 inhibits terminal differentiation
    Xinping Zhang
    Department of Orthopaedics, Center for Musculoskeletal Research, University of Rochester, Rochester, NY 14642, USA
    Bone 34:809-17. 2004
    ....
  18. ncbi request reprint Runx3/AML2/Cbfa3 regulates early and late chondrocyte differentiation
    Do Y Soung
    Center for Musculoskeletal Research, University of Rochester, Rochester, New York 14642, USA
    J Bone Miner Res 22:1260-70. 2007
    ..We found that Runx3 is essential for mediating the early stage of endochondral ossification through cooperation with other Runx family members...
  19. ncbi request reprint ALK2 functions as a BMP type I receptor and induces Indian hedgehog in chondrocytes during skeletal development
    Donghui Zhang
    Center for Musculoskeletal Research, University of Rochester, Rochester, New York 14642, USA
    J Bone Miner Res 18:1593-604. 2003
    ..The findings suggest that BMP signaling modulates the Ihh/PTHrP signaling pathway that regulates the rate of chondrocyte differentiation...
  20. pmc Beta-catenin, cartilage, and osteoarthritis
    Qiuqian Wu
    Department of Orthopaedics and Rehabilitation, Center for Musculoskeletal Research, University of Rochester School of Medicine and Dentistry, Rochester, New York, USA
    Ann N Y Acad Sci 1192:344-50. 2010
    ..These findings indicate that beta-catenin plays a central role in articular cartilage function and that activation of beta-catenin signaling may represent a pathologic mechanism for OA development...
  21. ncbi request reprint Transforming growth factor-beta1 induced alteration of skeletal morphogenesis in vivo
    Cristin M Ferguson
    Department of Orthopaedics, Center for Musculoskeletal Research, University of Rochester School of Medicine and Dentistry, 601 Elmwood Ave, Box 665, Rochester, NY 14642, USA
    J Orthop Res 22:687-96. 2004
    ..The findings confirm a critical role for TGF-beta during skeletal development. A more complete understanding of the role of TGF-beta and its down-stream signals will lead to improved understanding and treatment of cartilage diseases...
  22. pmc Prostaglandin E2 inhibits BMP signaling and delays chondrocyte maturation
    Christine A Clark
    Center for Musculoskeletal Research, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA
    J Orthop Res 27:785-92. 2009
    ..18 cells and in primary murine sternal chondrocytes. Altogether, the findings show that PGE2 regulates chondrocyte maturation in part by targeting BMP/Smad signaling and suggest an important role for PGE2 in endochondral bone formation...
  23. pmc Transforming growth factor-beta stimulates cyclin D1 expression through activation of beta-catenin signaling in chondrocytes
    Tian Fang Li
    Department of Orthopaedics, Center for Musculoskeletal Research, University of Rochester, New York 14642, USA
    J Biol Chem 281:21296-304. 2006
    ..These results demonstrate that TGF-beta stimulates cyclin D(1) expression at least in part through activation of beta-catenin signaling...
  24. pmc Lead exposure inhibits fracture healing and is associated with increased chondrogenesis, delay in cartilage mineralization, and a decrease in osteoprogenitor frequency
    Jonathan J Carmouche
    Center for Musculoskeletal Research, University of Rochester Medical Center, University of Rochester, School of Medicine and Dentistry, Rochester, New York 14642, USA
    Environ Health Perspect 113:749-55. 2005
    ..We conclude that Pb delays fracture healing at environmentally relevant doses and induces fibrous nonunions at higher doses by inhibiting the progression of endochondral ossification...
  25. pmc Induction of an osteoarthritis-like phenotype and degradation of phosphorylated Smad3 by Smurf2 in transgenic mice
    Qiuqian Wu
    Department of Orthopaedics, University of Rochester Medical Center, Rochester, New York 14642, USA
    Arthritis Rheum 58:3132-44. 2008
    ..To determine whether Smurf2, an E3 ubiquitin ligase known to inhibit transforming growth factor beta (TGFbeta) signaling, is expressed in human osteoarthritic (OA) cartilage and can initiate OA in mice...
  26. ncbi request reprint ATF-2 cooperates with Smad3 to mediate TGF-beta effects on chondrocyte maturation
    Andreia M Ionescu
    Center for Musculoskeletal Research, School of Medicine and Dentistry, University of Rochester, Rochester, NY 14642, USA
    Exp Cell Res 288:198-207. 2003
    ..Thus, ATF-2 regulates chondrocyte maturation as a direct target of TGF-beta signaling while CREB regulates differentiation by targeting genes independent of the individual signaling effects of TGF-beta or BMP-2...
  27. pmc Smad3-deficient chondrocytes have enhanced BMP signaling and accelerated differentiation
    Tian Fang Li
    Center for Musculoskeletal Research, University of Rochester School of Medicine and Dentistry, Rochester, New York, USA
    J Bone Miner Res 21:4-16. 2006
    ..Smad2 or Smad3 overexpression and BMP blockade abrogate accelerated maturation in Smad3-/- chondrocytes. BMP signaling is increased in TGF-beta deficiency and is required for accelerated chondrocyte maturation...
  28. pmc Aberrant hypertrophy in Smad3-deficient murine chondrocytes is rescued by restoring transforming growth factor beta-activated kinase 1/activating transcription factor 2 signaling: a potential clinical implication for osteoarthritis
    Tian Fang Li
    University of Rochester, Rochester, New York 14642, USA
    Arthritis Rheum 62:2359-69. 2010
    ....
  29. ncbi request reprint Transcriptional regulation of chondrocyte maturation: potential involvement of transcription factors in OA pathogenesis
    Hicham Drissi
    The Center for Musculoskeletal Research, University of Rochester, Rochester, NY 14642, United States
    Mol Aspects Med 26:169-79. 2005
    ..Thus, unraveling the molecular interplay between various factors that regulate chondrocyte maturation during OA occurrence and progression is the main focus of ongoing efforts...
  30. pmc Smurf2 induces degradation of GSK-3beta and upregulates beta-catenin in chondrocytes: a potential mechanism for Smurf2-induced degeneration of articular cartilage
    Qiuqian Wu
    Center for Musculoskeletal Research, University of Rochester Medical Center, 601 Elmwood Avenue, Rochester, NY 14642, USA
    Exp Cell Res 315:2386-98. 2009
    ....
  31. pmc Regulation of embryonic endochondral ossification by Smurf2
    Qiuqian Wu
    Department of Orthopaedics, Center for Musculoskeletal Research, University of Rochester Medical Center, 601 Elmwood Avenue, Box 665, Rochester, New York 14642, USA
    J Orthop Res 26:704-12. 2008
    ....
  32. pmc Overexpression of Smurf2 stimulates endochondral ossification through upregulation of beta-catenin
    Qiuqian Wu
    Department of Orthopaedics, Center for Musculoskeletal Research, University of Rochester Medical Center, 601 Elmwood Avenue, Box 665, Rochester, New York 14642, USA
    J Bone Miner Res 23:552-63. 2008
    ..The mechanism underlying Smurf2-mediated morphological changes during embryonic development may provide new mechanistic insights and potential targets for prevention and treatment of human osteoarthritis...
  33. ncbi request reprint Smad7 mediates inhibition of Saos2 osteosarcoma cell differentiation by NFkappaB
    Roman A Eliseev
    Center for Musculoskeletal Research, University of Rochester Medical Center, Rochester, NY 14642, USA
    Exp Cell Res 312:40-50. 2006
    ..We therefore conclude that in osteosarcoma Saos2 cells, NFkappaB represses BMP/Smad signaling and BMP2-induced differentiation through Smad7...
  34. pmc RBPjkappa-dependent Notch signaling regulates mesenchymal progenitor cell proliferation and differentiation during skeletal development
    Yufeng Dong
    Department of Orthopaedics and Rehabilitation, Center for Musculoskeletal Research, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA
    Development 137:1461-71. 2010
    ..Finally, Hes1 was identified as an RBPjkappa-dependent Notch target gene important for MPC maintenance and the suppression of in vitro chondrogenesis...
  35. ncbi request reprint Smad6 is induced by BMP-2 and modulates chondrocyte differentiation
    Xeufeng Li
    Center for Musculoskeletal Research, Department of Orthopaedics, University of Rochester Medical Center, 601 Elmwood Avenue, Box 665, Rochester, NY 14642, USA
    J Orthop Res 21:908-13. 2003
    ..Additional studies are required to determine the extent to which this pathway participates in pathologic processes involving cartilage...
  36. pmc Lead induces chondrogenesis and alters transforming growth factor-beta and bone morphogenetic protein signaling in mesenchymal cell populations
    Michael J Zuscik
    Center for Musculoskeletal Research, University of Rochester School of Medicine and Dentistry, Rochester, New York, USA
    Environ Health Perspect 115:1276-82. 2007
    ..Because chondrogenesis is a seminal step during skeletal development, elucidating the impact of Pb on this process is the first step toward understanding the mechanism of Pb toxicity in the skeleton...
  37. ncbi request reprint A phage display technique identifies a novel regulator of cell differentiation
    Tzong Jen Sheu
    Department of Orthopaedics, Center for Musculoskeletal Research, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA
    J Biol Chem 278:438-43. 2003
    ..We hypothesize that binding to TRAP may be one mechanism by which the full osteoblast phenotype is expressed during the process of bone remodeling...
  38. pmc Regulation of chondrogenesis and chondrocyte differentiation by stress
    Michael J Zuscik
    Center for Musculoskeletal Research, University of Rochester Medical Center, Rochester, New York 14642, USA
    J Clin Invest 118:429-38. 2008
    ..A summary of the influence of the most well-understood normal and pathologic stressors on the differentiation program is also presented...
  39. pmc GPCR kinase 2 interacting protein 1 (GIT1) regulates osteoclast function and bone mass
    Prashanthi Menon
    Department of Medicine and Aab Cardiovascular Research Institute, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA
    J Cell Physiol 225:777-85. 2010
    ..These data show that GIT1 is a key regulator of bone mass in vivo by regulating osteoclast function and suggest GIT1 as a potential target for osteoporosis therapy...
  40. ncbi request reprint Environmental agents affect skeletal growth and development
    Jonathan D Holz
    Department of Orthopaedics and the Center for Musculoskeletal Research, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA
    Birth Defects Res C Embryo Today 81:41-50. 2007
    ..Research in this area will identify strategies that may be used to help prevent musculoskeletal diseases due to toxicant exposure...
  41. ncbi request reprint Differential regulation of EP receptor isoforms during chondrogenesis and chondrocyte maturation
    Christine A Clark
    Center for Musculoskeletal Research, University of Rochester, School of Medicine and Dentistry, Rochester, NY 14642, USA
    Biochem Biophys Res Commun 328:764-76. 2005
    ....
  42. ncbi request reprint Increased radiation-induced apoptosis of Saos2 cells via inhibition of NFkappaB: a role for c-Jun N-terminal kinase
    Roman A Eliseev
    Center for Musculoskeletal Research, University of Rochester Medical Center, Rochester, New York 14642, USA
    J Cell Biochem 96:1262-73. 2005
    ..Our study brings new insight into the mechanisms underlying radiation-induced apoptosis of osteosarcoma, and may lead to development of new therapeutic strategies against osteosarcoma...
  43. pmc Reduced COX-2 expression in aged mice is associated with impaired fracture healing
    Amish A Naik
    The Center for Musculoskeletal Research, University of Rochester, Rochester, New York 14642, USA
    J Bone Miner Res 24:251-64. 2009
    ..The findings suggest that COX-2/EP4 agonists may compensate for deficient molecular signals that result in the reduced fracture healing associated with aging...
  44. ncbi request reprint Gene expression profiling of alpha(1b)-adrenergic receptor-induced cardiac hypertrophy by oligonucleotide arrays
    June Yun
    The Department of Molecular Cardiology NB50, The Lerner Research Institute, The Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, OH 44195, USA
    Cardiovasc Res 57:443-55. 2003
    ..We wanted to explore potential gene expression differences that characterize this type of hypertrophy that may identify genes that prevent progression to heart failure...
  45. ncbi request reprint Mice expressing the alpha(1B)-adrenergic receptor induces a synucleinopathy with excessive tyrosine nitration but decreased phosphorylation
    Robert Papay
    Department of Molecular Cardiology, The Lerner Research Institute, The Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, OH 44195, USA
    J Neurochem 83:623-34. 2002
    ..We conclude that overexpression of the alpha(1B)-AR can cause a synucleinopathy similar to other parkinsonian syndromes...
  46. ncbi request reprint Altered negative regulation of transforming growth factor beta signaling in scleroderma: potential involvement of SMURF2 in disease
    Michael J Zuscik
    Arthritis Rheum 48:1779-80. 2003

Research Grants4

  1. Mechanism of Nicotine Action in the Healing Skeleton
    Michael Zuscik; Fiscal Year: 2007
    ....
  2. Mechanism of Nicotine Action in the Healing Skeleton
    Michael Zuscik; Fiscal Year: 2009
    ....