A Zlotnick

Summary

Affiliation: University of Oklahoma Health Sciences Center
Country: USA

Publications

  1. ncbi A theoretical model successfully identifies features of hepatitis B virus capsid assembly
    A Zlotnick
    Department of Biochemistry and Molecular Biology, Oklahoma University Health Sciences Center, Oklahoma City, Oklahoma 73190, USA
    Biochemistry 38:14644-52. 1999
  2. ncbi Theoretical aspects of virus capsid assembly
    Adam Zlotnick
    Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA
    J Mol Recognit 18:479-90. 2005
  3. ncbi How does your virus grow? Understanding and interfering with virus assembly
    Adam Zlotnick
    Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73190, USA
    Trends Biotechnol 21:536-42. 2003
  4. ncbi Are weak protein-protein interactions the general rule in capsid assembly?
    Adam Zlotnick
    University of Oklahoma Health Sciences Center, Oklahoma City, OK 73190, USA
    Virology 315:269-74. 2003
  5. pmc Viruses and the physics of soft condensed matter
    Adam Zlotnick
    Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73190, USA
    Proc Natl Acad Sci U S A 101:15549-50. 2004
  6. pmc Distinguishing reversible from irreversible virus capsid assembly
    Adam Zlotnick
    Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA
    J Mol Biol 366:14-8. 2007
  7. pmc In vitro screening for molecules that affect virus capsid assembly (and other protein association reactions)
    Adam Zlotnick
    Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73104, USA
    Nat Protoc 2:490-8. 2007
  8. pmc A small molecule inhibits and misdirects assembly of hepatitis B virus capsids
    Adam Zlotnick
    Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73190, USA
    J Virol 76:4848-54. 2002
  9. ncbi Mechanism of capsid assembly for an icosahedral plant virus
    A Zlotnick
    Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73190, USA
    Virology 277:450-6. 2000
  10. ncbi An in vitro fluorescence screen to identify antivirals that disrupt hepatitis B virus capsid assembly
    Stephen J Stray
    Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, 975 NE 10th St BRC 456, Oklahoma City, Oklahoma 73104, USA
    Nat Biotechnol 24:358-62. 2006

Research Grants

  1. RPA STRUCTURE AND MECHANISM OF INTERACTION WITH SSDNA
    Adam Zlotnick; Fiscal Year: 2004
  2. Molecular Modulation of HBV Capsid Assembly
    Adam Zlotnick; Fiscal Year: 2007
  3. Regulation of Virus Capsid Assembly
    GILLIAN AIR; Fiscal Year: 2007

Collaborators

  • Brian P Bothner
  • John Johnson
  • Stephen J Stray
  • Santanu Mukherjee
  • GILLIAN AIR
  • Christina R Bourne
  • Pablo Ceres
  • Paul Moisant
  • Dan Endres
  • M G Finn
  • Kevin Burns
  • J Zachary Porterfield
  • Jonathan K Hilmer
  • Christina Bourne
  • Jinghua Tang
  • Kristin N Parent
  • Sushmita Singh
  • Thomas Keef
  • Ariella Oppenheim
  • Henry Neeman
  • Stanislav Kler
  • Matthew R Fulz
  • Sarah P Katen
  • Charles Packianathan
  • Brent Korba
  • Bollu Venkataiah
  • Lauren Beth Johnston
  • Marc V Thorsteinsson
  • Sejin Lee
  • Pete A DePhillips
  • Angela Lee
  • Cory M Pfeifer
  • Kelly A Dryden
  • Benjamin G Kopek
  • Carolyn M Teschke
  • Jay Liu
  • Mark J Young
  • Sreenivas Punna
  • Masaki Miyahara
  • Warren G Lewis

Detail Information

Publications32

  1. ncbi A theoretical model successfully identifies features of hepatitis B virus capsid assembly
    A Zlotnick
    Department of Biochemistry and Molecular Biology, Oklahoma University Health Sciences Center, Oklahoma City, Oklahoma 73190, USA
    Biochemistry 38:14644-52. 1999
    ..On the basis of this mechanism, we suggest that HBV capsid assembly could be an important target for antiviral therapeutics...
  2. ncbi Theoretical aspects of virus capsid assembly
    Adam Zlotnick
    Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA
    J Mol Recognit 18:479-90. 2005
    ..The capsid protein must polymerize in order to create a three-dimensional protein polymer. More than structure is required to understand this self-assembly reaction: one must understand how the pieces come together in solution...
  3. ncbi How does your virus grow? Understanding and interfering with virus assembly
    Adam Zlotnick
    Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73190, USA
    Trends Biotechnol 21:536-42. 2003
  4. ncbi Are weak protein-protein interactions the general rule in capsid assembly?
    Adam Zlotnick
    University of Oklahoma Health Sciences Center, Oklahoma City, OK 73190, USA
    Virology 315:269-74. 2003
  5. pmc Viruses and the physics of soft condensed matter
    Adam Zlotnick
    Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73190, USA
    Proc Natl Acad Sci U S A 101:15549-50. 2004
  6. pmc Distinguishing reversible from irreversible virus capsid assembly
    Adam Zlotnick
    Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA
    J Mol Biol 366:14-8. 2007
    ..Data in the literature strongly support the hypothesis that most viruses are held together by many weak interactions...
  7. pmc In vitro screening for molecules that affect virus capsid assembly (and other protein association reactions)
    Adam Zlotnick
    Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73104, USA
    Nat Protoc 2:490-8. 2007
    ..With manual pipetting, setting up a plate takes about 2 h, the initial reading takes 1 h and the end point reading the following day takes about 5 min...
  8. pmc A small molecule inhibits and misdirects assembly of hepatitis B virus capsids
    Adam Zlotnick
    Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73190, USA
    J Virol 76:4848-54. 2002
    ..We propose that bis-ANS acts as a molecular "wedge" that interferes with normal capsid protein geometry and capsid formation; such wedges may represent a new class of antiviral agent...
  9. ncbi Mechanism of capsid assembly for an icosahedral plant virus
    A Zlotnick
    Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73190, USA
    Virology 277:450-6. 2000
    ....
  10. ncbi An in vitro fluorescence screen to identify antivirals that disrupt hepatitis B virus capsid assembly
    Stephen J Stray
    Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, 975 NE 10th St BRC 456, Oklahoma City, Oklahoma 73104, USA
    Nat Biotechnol 24:358-62. 2006
    ..Our approach may facilitate the identification of antivirals targeting viruses other than HBV, such as avian influenza and HIV...
  11. pmc A mutant hepatitis B virus core protein mimics inhibitors of icosahedral capsid self-assembly
    Christina R Bourne
    Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73104, USA
    Biochemistry 48:1736-42. 2009
    ..Thus, attempts to control virus self-assembly (with small molecules or defective proteins) must take into account the competing process of thermodynamic editing...
  12. ncbi Observed hysteresis of virus capsid disassembly is implicit in kinetic models of assembly
    Sushmita Singh
    Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73190, USA
    J Biol Chem 278:18249-55. 2003
    ..quot; A biological implication of enhanced stability is that a triggering event may be required to initiate virus uncoating...
  13. pmc Hepatitis B virus capsid assembly is enhanced by naturally occurring mutation F97L
    Pablo Ceres
    Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73190, USA
    J Virol 78:9538-43. 2004
    ..Since the structures of the mutant and wild-type capsids are essentially the same and the mutation is not involved in the contact between dimers, we suggest that the F97L mutation affects the dynamic behavior of dimer and capsid...
  14. ncbi Weak protein-protein interactions are sufficient to drive assembly of hepatitis B virus capsids
    Pablo Ceres
    Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73190, USA
    Biochemistry 41:11525-31. 2002
    ..This model of conformational change linked to assembly is consistent with immunological differences between dimer and capsid...
  15. pmc A heteroaryldihydropyrimidine activates and can misdirect hepatitis B virus capsid assembly
    Stephen J Stray
    Department of Biochemistry and Molecular Biology BRC 464, University of Oklahoma Health Sciences Center, P O Box 26901, Oklahoma City, OK 73190, USA
    Proc Natl Acad Sci U S A 102:8138-43. 2005
    ..HAP compounds may have multiple effects in vivo stemming from inappropriate assembly of Cp. These results show that activating and deregulating virus assembly may be a powerful general approach for antiviral therapeutics...
  16. ncbi BAY 41-4109 has multiple effects on Hepatitis B virus capsid assembly
    Stephen J Stray
    Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73190, USA
    J Mol Recognit 19:542-8. 2006
    ..Our data suggest that HAP compounds may inhibit virus replication by inducing assembly inappropriately and, when in excess, by misdirecting assembly decreasing the stability of normal capsids...
  17. pmc Global structural changes in hepatitis B virus capsids induced by the assembly effector HAP1
    Christina R Bourne
    Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, 975 E 10th Street, Oklahoma City, Oklahoma 73104, USA
    J Virol 80:11055-61. 2006
    ..These results demonstrate the plasticity of HBV capsids and the molecular basis for a tenable antiviral strategy...
  18. pmc A simple and general method for determining the protein and nucleic acid content of viruses by UV absorbance
    J Zachary Porterfield
    Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73104, USA
    Virology 407:281-8. 2010
    ..The RNA:protein charge ratio (about 1.4 phosphates per positive charge) was consistent with that of other ssRNA viruses. This spectroscopic method is generalizable to any virus-like particle...
  19. pmc Exploring the paths of (virus) assembly
    Paul Moisant
    Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, USA
    Biophys J 99:1350-7. 2010
    ....
  20. ncbi Interaction with capsid protein alters RNA structure and the pathway for in vitro assembly of cowpea chlorotic mottle virus
    Jennifer M Johnson
    Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73190, USA
    J Mol Biol 335:455-64. 2004
    ..This mechanism, observed in a plant virus, may be applicable to other viruses that do not halt synthesis of host nucleic acid, including HIV...
  21. ncbi Zinc ions trigger conformational change and oligomerization of hepatitis B virus capsid protein
    Stephen J Stray
    Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, P O Box 26901, Oklahoma City, Oklahoma 73190, USA
    Biochemistry 43:9989-98. 2004
    ..Free zinc ions may not be the true in vivo activator of assembly, but they provide a model for regulation of assembly...
  22. ncbi Regulating self-assembly of spherical oligomers
    Jennifer M Johnson
    Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73190, USA
    Nano Lett 5:765-70. 2005
    ..Altering the assembly path of "spherical" particles is a means of controlling the distribution of products, which has broad applicability to self-assembly reactions...
  23. doi Altering the energy landscape of virus self-assembly to generate kinetically trapped nanoparticles
    Kevin Burns
    Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, 73104, USA
    Biomacromolecules 11:439-42. 2010
    ..The extrusion of all-pentamer capsids from the hexagonal lattice of the rod demonstrates the importance of the starting state for controlled assembly...
  24. doi Uncatalyzed assembly of spherical particles from SV40 VP1 pentamers and linear dsDNA incorporates both low and high cooperativity elements
    Santanu Mukherjee
    Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA
    Virology 397:199-204. 2010
    ..These data suggest a DNA saturation-dependent nucleation event based on non-specific pentamer-DNA interaction that controls assembly and the ultimate capsid geometry...
  25. doi A quantitative description of in vitro assembly of human papillomavirus 16 virus-like particles
    Santanu Mukherjee
    Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA
    J Mol Biol 381:229-37. 2008
    ..2 disassembled slower than pH 5.2, 6.2, or 8.2 VLPs. We hypothesize that pH 7.2 assembly reactions lead to formation of particles with conformationally different interactions...
  26. ncbi Redirecting the coat protein of a spherical virus to assemble into tubular nanostructures
    Santanu Mukherjee
    Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, 73104, USA
    J Am Chem Soc 128:2538-9. 2006
    ..Critical to redirecting assembly are the weak interprotein association energy inherent to virus assembly and the relatively rigid nature of the double-stranded DNA scaffold at their core...
  27. pmc Small-molecule effectors of hepatitis B virus capsid assembly give insight into virus life cycle
    Christina Bourne
    Indiana University, Department of Biology, Simon Hall MSB, Room 220D, 212 S Hawthorne Drive, Bloomington, IN 47405 7003, USA
    J Virol 82:10262-70. 2008
    ..This is a predictive and testable model for the mechanism of action of assembly effectors...
  28. pmc Model-based analysis of assembly kinetics for virus capsids or other spherical polymers
    Dan Endres
    Department of Mathematics and Statistics, University of Central Oklahoma, Edmond, Oklahoma 73034, USA
    Biophys J 83:1217-30. 2002
    ..This enhanced understanding of virus assembly will allow a more quantitative analysis of virus stability and biological or antiviral factors that affect assembly...
  29. ncbi Quantitative analysis of multi-component spherical virus assembly: scaffolding protein contributes to the global stability of phage P22 procapsids
    Kristin N Parent
    Department of Molecular and Cell Biology, University of Connecticut, Storrs, CT 06269 3125, USA
    J Mol Biol 359:1097-106. 2006
    ..When the relative concentration is very high, too many nuclei form, leading to kinetically trapped assembly intermediates...
  30. pmc Conformational equilibria and rates of localized motion within hepatitis B virus capsids
    Jonathan K Hilmer
    Montana State University, Department of Chemistry and Biochemistry, Chemistry and Biochemistry Building, Bozeman, MT 59717, USA
    J Mol Biol 375:581-94. 2008
    ....
  31. pmc A reaction landscape identifies the intermediates critical for self-assembly of virus capsids and other polyhedral structures
    Dan Endres
    Department of Mathematics and Statistics, University of Central Oklahoma, Edmond 73034, USA
    Protein Sci 14:1518-25. 2005
    ..This result suggests that only a minute fraction of intermediates needs to be considered when describing capsid assembly...
  32. ncbi The role of subunit hinges and molecular "switches" in the control of viral capsid polymorphism
    Jinghua Tang
    Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    J Struct Biol 154:59-67. 2006
    ..These results show that capsid geometry is flexible and may readily adapt to new requirements as the virus evolves...

Research Grants5

  1. RPA STRUCTURE AND MECHANISM OF INTERACTION WITH SSDNA
    Adam Zlotnick; Fiscal Year: 2004
    ..4. What is the general mechanism of ssDNA-binding by the RPA trimer? A trimeric RPA complex containing all ssDNA-binding domains will be resolved by X-ray crystallography with and/or without ssDNA. ..
  2. Molecular Modulation of HBV Capsid Assembly
    Adam Zlotnick; Fiscal Year: 2007
    ..25 million Americans. Worldwide, HBV will contribute to 1 million deaths this year. Current antiviral strategies focus on virus enzymes. We propose a new strategy for developing antiviral molecules that target virus assembly. ..
  3. Regulation of Virus Capsid Assembly
    GILLIAN AIR; Fiscal Year: 2007
    ..25 million Americans. Worldwide, HBV will contribute to 1 million deaths this year. By determining how HBV assembly is regulated, we will characterize a new target for antiviral intervention. ..