V P Zharov

Summary

Affiliation: University of Arkansas for Medical Sciences
Country: USA

Publications

  1. ncbi request reprint Covalently linked Au nanoparticles to a viral vector: potential for combined photothermal and gene cancer therapy
    Maaike Everts
    Division of Human Gene Therapy, Department of Medicine, Gene Therapy Center, University of Alabama, Birmingham, Alabama 35294, USA
    Nano Lett 6:587-91. 2006
  2. ncbi request reprint Photothermal flow cytometry in vitro for detection and imaging of individual moving cells
    Vladimir P Zharov
    Philips Classic Laser Laboratories, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205 7199, USA
    Cytometry A 71:191-206. 2007
  3. ncbi request reprint Photothermal antimicrobial nanotherapy and nanodiagnostics with self-assembling carbon nanotube clusters
    Jin Woo Kim
    Department of Biological and Agricultural Engineering, University of Arkansas, Fayetteville, AR 72701, USA
    Lasers Surg Med 39:622-34. 2007
  4. pmc Ultra-fast photoacoustic flow cytometry with a 0.5 MHz pulse repetition rate nanosecond laser
    Dmitry A Nedosekin
    Phillips Classic Laser and Nanomedicine Laboratories, University of Arkansas for Medical Sciences, 4301 W Markham St, Little Rock, AR, 72205, USA
    Opt Express 18:8605-20. 2010
  5. pmc Photothermal and photoacoustic Raman cytometry in vitro and in vivo
    Evgeny V Shashkov
    Phillips Classic Laser and Nanomedicine Laboratories, University of Arkansas for Medical Sciences, 4301 W Markham, Little Rock, AR 72205, USA
    Opt Express 18:6929-44. 2010
  6. pmc Photothermal nanodrugs: potential of TNF-gold nanospheres for cancer theranostics
    Jingwei Shao
    College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou, Fujian, China
    Sci Rep 3:1293. 2013
  7. pmc In vivo multispectral photoacoustic and photothermal flow cytometry with multicolor dyes: a potential for real-time assessment of circulation, dye-cell interaction, and blood volume
    Mikhail A Proskurnin
    Chemistry Department, M V Lomonosov Moscow State University, Moscow, Russia
    Cytometry A 79:834-47. 2011
  8. ncbi request reprint Photoacoustic flow cytometry: principle and application for real-time detection of circulating single nanoparticles, pathogens, and contrast dyes in vivo
    Vladimir P Zharov
    University of Arkansas for Medical Sciences, Phillips Classic Laser Laboratories, Little Rock, Arkansas 72205, USA
    J Biomed Opt 12:051503. 2007
  9. ncbi request reprint Infrared imaging of subcutaneous veins
    Vladimir P Zharov
    Philips Classic Laser Biomedical Laboratory, University of Arkansas for Medical Sciences, 4301 West Markham Street, Slot 543, Little Rock, AR 72205 7199, USA
    Lasers Surg Med 34:56-61. 2004
  10. ncbi request reprint In vivo photoacoustic flow cytometry for monitoring of circulating single cancer cells and contrast agents
    Vladimir P Zharov
    University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205, USA
    Opt Lett 31:3623-5. 2006

Research Grants

  1. In vivo real-time detection of circulating melanoma cells
    Vladimir P Zharov; Fiscal Year: 2010
  2. Photothermal Evaluation of Drug Toxicity at Cell Level
    Vladimir Zharov; Fiscal Year: 2004
  3. Multifunctional nanotubes for in vivo detecting/purging circulating cancer cells
    Vladimir P Zharov; Fiscal Year: 2010
  4. Multifunctional nanotubes for in vivo detecting/purging circulating cancer cells
    Vladimir Zharov; Fiscal Year: 2009
  5. In vivo real-time detection of circulating melanoma cells
    Vladimir Zharov; Fiscal Year: 2009
  6. Integrated Photothermal Nanoprobes
    Vladimir Zharov; Fiscal Year: 2007
  7. Photothermal Imaging of Live Cells
    Vladimir Zharov; Fiscal Year: 2007
  8. Photothermal Imaging of Live Cells
    Vladimir Zharov; Fiscal Year: 2006
  9. Photothermal Imaging of Live Cells
    Vladimir Zharov; Fiscal Year: 2005
  10. Integrated optical imaging rat of mesentery
    Vladimir Zharov; Fiscal Year: 2004

Collaborators

Detail Information

Publications43

  1. ncbi request reprint Covalently linked Au nanoparticles to a viral vector: potential for combined photothermal and gene cancer therapy
    Maaike Everts
    Division of Human Gene Therapy, Department of Medicine, Gene Therapy Center, University of Alabama, Birmingham, Alabama 35294, USA
    Nano Lett 6:587-91. 2006
    ..These results indicate the possibility of using adenoviral vectors as carriers for gold nanoparticles...
  2. ncbi request reprint Photothermal flow cytometry in vitro for detection and imaging of individual moving cells
    Vladimir P Zharov
    Philips Classic Laser Laboratories, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205 7199, USA
    Cytometry A 71:191-206. 2007
    ..The goal of our investigation was to expand this technique to the detection of flowing cells in vitro...
  3. ncbi request reprint Photothermal antimicrobial nanotherapy and nanodiagnostics with self-assembling carbon nanotube clusters
    Jin Woo Kim
    Department of Biological and Agricultural Engineering, University of Arkansas, Fayetteville, AR 72701, USA
    Lasers Surg Med 39:622-34. 2007
    ..In this study, new CNTs' capabilities for photothermal (PT) antimicrobial nanotherapy were explored in vitro using Escherichia coli as a model bacterium...
  4. pmc Ultra-fast photoacoustic flow cytometry with a 0.5 MHz pulse repetition rate nanosecond laser
    Dmitry A Nedosekin
    Phillips Classic Laser and Nanomedicine Laboratories, University of Arkansas for Medical Sciences, 4301 W Markham St, Little Rock, AR, 72205, USA
    Opt Express 18:8605-20. 2010
    ..5 m/s...
  5. pmc Photothermal and photoacoustic Raman cytometry in vitro and in vivo
    Evgeny V Shashkov
    Phillips Classic Laser and Nanomedicine Laboratories, University of Arkansas for Medical Sciences, 4301 W Markham, Little Rock, AR 72205, USA
    Opt Express 18:6929-44. 2010
    ....
  6. pmc Photothermal nanodrugs: potential of TNF-gold nanospheres for cancer theranostics
    Jingwei Shao
    College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou, Fujian, China
    Sci Rep 3:1293. 2013
    ....
  7. pmc In vivo multispectral photoacoustic and photothermal flow cytometry with multicolor dyes: a potential for real-time assessment of circulation, dye-cell interaction, and blood volume
    Mikhail A Proskurnin
    Chemistry Department, M V Lomonosov Moscow State University, Moscow, Russia
    Cytometry A 79:834-47. 2011
    ..The potential for developing a robust clinical PAFC prototype that is safe for human, and its applications for studying the liver function are further highlighted...
  8. ncbi request reprint Photoacoustic flow cytometry: principle and application for real-time detection of circulating single nanoparticles, pathogens, and contrast dyes in vivo
    Vladimir P Zharov
    University of Arkansas for Medical Sciences, Phillips Classic Laser Laboratories, Little Rock, Arkansas 72205, USA
    J Biomed Opt 12:051503. 2007
    ....
  9. ncbi request reprint Infrared imaging of subcutaneous veins
    Vladimir P Zharov
    Philips Classic Laser Biomedical Laboratory, University of Arkansas for Medical Sciences, 4301 West Markham Street, Slot 543, Little Rock, AR 72205 7199, USA
    Lasers Surg Med 34:56-61. 2004
    ..Here we revisited and explored the capability of near-IR imaging to visualize subcutaneous structures, with a focus on diagnostics of superficial veins...
  10. ncbi request reprint In vivo photoacoustic flow cytometry for monitoring of circulating single cancer cells and contrast agents
    Vladimir P Zharov
    University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205, USA
    Opt Lett 31:3623-5. 2006
    ..The threshold sensitivity is estimated as one cancer cell in the background of 10(7) normal blood cells...
  11. ncbi request reprint Self-assembling nanoclusters in living systems: application for integrated photothermal nanodiagnostics and nanotherapy
    Vladimir P Zharov
    Philips Classic Laser Laboratories, The Arkansas Cancer Research Center, The University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA
    Nanomedicine 1:326-45. 2005
    ....
  12. ncbi request reprint In vivo photothermal flow cytometry: imaging and detection of individual cells in blood and lymph flow
    Vladimir P Zharov
    Philips Classic Laser Laboratories, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205, USA
    J Cell Biochem 97:916-32. 2006
    ..g., drug or radiation), in natural biological environments...
  13. ncbi request reprint Integrated photothermal flow cytometry in vivo
    Vladimir P Zharov
    University of Arkansas for Medical Sciences, Philips Classic Laser Laboratories, 4301 West Markham St 543, Little Rock, Arkansas 72205 7199, USA
    J Biomed Opt 10:051502. 2005
    ....
  14. pmc Photothermal nanotherapeutics and nanodiagnostics for selective killing of bacteria targeted with gold nanoparticles
    Vladimir P Zharov
    Philips Classic Laser Laboratories, and Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA
    Biophys J 90:619-27. 2006
    ..1-5 J/cm(2), 100 pulses), and laser-induced bacterial damage observed at different laser fluences and nanoparticle sizes was verified by optical transmission, electron microscopy, and conventional viability testing...
  15. ncbi request reprint Synergistic enhancement of selective nanophotothermolysis with gold nanoclusters: potential for cancer therapy
    Vladimir P Zharov
    Philips Classic Laser Laboratories, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205, USA
    Lasers Surg Med 37:219-26. 2005
    ..We developed a new approach that enhances selective photothermolysis of tumor through laser activation of synergistic phenomena around nanoclusters, which are self-assembled into cancer cells...
  16. ncbi request reprint Nanocluster model of photothermal assay: application for high-sensitive monitoring of nicotine-induced changes in metabolism, apoptosis, and necrosis at a cellular level
    Vladimir P Zharov
    University of Arkansas for Medical Sciences, Philips Classic Laser Laboratories, 4301 West Markham Street, Number 543, Little Rock, Arkansas 72205 7199, USA
    J Biomed Opt 10:44011. 2005
    ..Comparison of PT responses of pancreatic tumor cells AR42J with isolated primary pancreatic acinar cells and HepG2 cells shows a universal nature of PT assay...
  17. ncbi request reprint Photothermal image flow cytometry in vivo
    Vladimir P Zharov
    Philips Classic Laser Laboratories, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205, USA
    Opt Lett 30:628-30. 2005
    ..Potential applications of this optical tool, called PT flow cytometry, are discussed...
  18. ncbi request reprint Design and application of low-frequency ultrasound and its combination with laser radiation in surgery and therapy
    V P Zharov
    Biomedical Technical Systems Department, Biomedical Engineering Faculty, N E Bauman Moscow State University of Technology, Russia
    Crit Rev Biomed Eng 29:502-19. 2001
    ..Some of the above-mentioned technologies were tested experimentally, and the results obtained are considered...
  19. ncbi request reprint Far-field photothermal microscopy beyond the diffraction limit
    Vladimir P Zharov
    Philips Classic Laser Biomedical Laboratory, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205 7199, USA
    Opt Lett 28:1314-6. 2003
    ..Algorithms for obtaining information on the sizes and the shapes of nanotargets are analyzed. Spectral PT lifetime imaging is suggested to provide spatial localization of nanotargets overlapping inside the diffraction volume...
  20. pmc In vivo photoacoustic time-of-flight velocity measurement of single cells and nanoparticles
    M Sarimollaoglu
    Winthrop P Rockefeller Cancer Institute, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205, USA
    Opt Lett 36:4086-8. 2011
    ..1 mm/s to 14 cm/s. Object velocity can be used to identify single cells circulating at different velocities or cell aggregates and to determine a cell's location in a vessel cross-section...
  21. ncbi request reprint Methods for photoultrasonic treatment of festering wounds in oncological patients
    V P Zharov
    Biomedical Technical Systems Department, N E Bauman Moscow State University of Technology, Russia
    Crit Rev Biomed Eng 29:111-24. 2001
    ..Moreover, this article describes the preliminary experimental results of the aforementioned technology...
  22. pmc In vivo magnetic enrichment and multiplex photoacoustic detection of circulating tumour cells
    Ekaterina I Galanzha
    Phillips Classic Laser and Nanomedicine Laboratories, Winthrop P Rockefeller Cancer Institute, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA
    Nat Nanotechnol 4:855-60. 2009
    ....
  23. ncbi request reprint Photothermal detection of nicotine-induced apoptotic effects in pancreatic cancer cells
    Valentin Galitovsky
    Philips Classic Laser Biomedical Laboratory, University of Arkansas for Medical Sciences UAMS, 4301 West Markham St, Slot 543, Little Rock, AR 72205, USA
    Life Sci 75:2677-87. 2004
    ..Thus, the PT assay demonstrated the capability for high-sensitivity detection of nicotine's impact, which may be related to a change in cell metabolism, apoptosis, or necrosis, depending on nicotine concentration...
  24. pmc In vivo multispectral, multiparameter, photoacoustic lymph flow cytometry with natural cell focusing, label-free detection and multicolor nanoparticle probes
    Ekaterina I Galanzha
    University of Arkansas for Medical Sciences, Philips Classic Laser Laboratories, Winthrop P Rockefeller Cancer Institute, Little Rock, Arkansas 72205, USA
    Cytometry A 73:884-94. 2008
    ....
  25. doi request reprint In vivo Raman flow cytometry for real-time detection of carbon nanotube kinetics in lymph, blood, and tissues
    Alexandru S Biris
    University of Arkansas at Little Rock, Applied Science Department, Nanotechnology Center, Little Rock, Arkansas 72204, USA
    J Biomed Opt 14:021006. 2009
    ..Moreover, this technology has the potential for molecular detection and identification of circulating tumor cells, and infections labeled with CNTs...
  26. pmc In vivo fiber-based multicolor photoacoustic detection and photothermal purging of metastasis in sentinel lymph nodes targeted by nanoparticles
    Ekaterina I Galanzha
    Winthrop P Rockefeller Cancer Institute, Phillips Classic Laser and Nanomedicine Laboratories, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA
    J Biophotonics 2:528-39. 2009
    ..The earliest detection of DTCs in the lymphatic vessels before the establishment of nodal metastasis may prevent metastasis by well-timed ablation of DTCs...
  27. pmc Nanotechnology-based molecular photoacoustic and photothermal flow cytometry platform for in-vivo detection and killing of circulating cancer stem cells
    Ekaterina I Galanzha
    Department of Biological and Agricultural Engineering and Institute for Nanoscale Materials Science and Engineering, University of Arkansas, Fayetteville, AR, 72701, USA
    J Biophotonics 2:725-35. 2009
    ....
  28. doi request reprint Nanophotothermolysis of multiple scattered cancer cells with carbon nanotubes guided by time-resolved infrared thermal imaging
    Alexandru S Biris
    University of Arkansas at Little Rock, Applied Science Department, Nanotechnology Center, Little Rock, Arkansas 72204, USA
    J Biomed Opt 14:021007. 2009
    ..The applications are discussed with a focus on the nanophotothermolysis of small tumors, tumor margins, or micrometastases under the guidance of near-IR and microwave radiometry...
  29. ncbi request reprint In vivo integrated flow image cytometry and lymph/blood vessels dynamic microscopy
    Ekaterina I Galanzha
    University of Arkansas for Medical Sciences, Philips Classic Laser Laboratories, Little Rock, Arkansas 72205 7199, USA
    J Biomed Opt 10:054018. 2005
    ....
  30. pmc Advances in small animal mesentery models for in vivo flow cytometry, dynamic microscopy, and drug screening
    Ekaterina I Galanzha
    Philips Classic Laser Laboratories, University of Arkansas for Medical Sciences, 4301 W Markham St, Little Rock, AR 72205 7199, United States
    World J Gastroenterol 13:192-218. 2007
    ..Possible future applications, including in vivo early diagnosis and prevention of disease, monitoring immune response and apoptosis, chemo- and radio-sensitivity tests, and drug screening, are also discussed...
  31. ncbi request reprint Laser non-contact drug delivery methods: mathematical and experimental substantiation
    V P Zharov
    Biomedical Technical Systems Department, N E Bauman Moscow State University of Technology, Russia
    Crit Rev Biomed Eng 29:142-56. 2001
    ..These preparations were injected into gelatinous gel and autopsy skin samples. Possible clinical application is considered for these laser transcutaneous drug delivery methods...
  32. ncbi request reprint Superhigh-sensitivity photothermal monitoring of individual cell response to antitumor drug
    Vladimir P Zharov
    University of Arkansas for Medical Sciences, Philips Classic Laser Laboratories, Little Rock, Arkansas 72205 7199, USA
    J Biomed Opt 11:064034. 2006
    ....
  33. ncbi request reprint Monitoring of nicotine impact in microlymphatics of rat mesentery with time-resolved microscopy
    E L Galanzha
    Philips Classic Laser Laboratories, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205, USA
    Lymphology 38:181-92. 2005
    ..We conclude that the observed effects are due to the direct action of nicotine on lymphatics. The mechanism by which nicotine induces this response remains to be investigated further...
  34. pmc Photothermal confocal spectromicroscopy of multiple cellular chromophores and fluorophores
    Dmitry A Nedosekin
    Phillips Classic Laser and Nanomedicine Laboratories, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA
    Biophys J 102:672-81. 2012
    ..Confocal PT spectromicroscopy could provide a valuable alternative or supplement to fluorescence microscopy for imaging of nonfluorescent chromophores and certain fluorophores...
  35. ncbi request reprint Optical monitoring of microlymphatic disturbances during experimental lymphedema
    E I Galanzha
    Philips Classic Laser Laboratories, University of Arkansas for Medical Sciences UAMS, Little Rock, Arkansas 72205 7199, USA
    Lymphat Res Biol 5:11-27. 2007
    ..Rat mesentery has been widely used to study microvascular functions. The goal of this work is to extend this animal model to monitor blood and lymph microvessel function during lymphedema...
  36. pmc Quantum dots as multimodal photoacoustic and photothermal contrast agents
    Evgeny V Shashkov
    Philips Classic Laser Laboratories, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205, USA
    Nano Lett 8:3953-8. 2008
    ..Further improvements for this technology are suggested by increasing the conversion of laser energy in PT, PA, and bubble phenomena in hybrid multilayer QDs that have optimized absorption, thermal, and acoustic properties...
  37. pmc In vivo, noninvasive, label-free detection and eradication of circulating metastatic melanoma cells using two-color photoacoustic flow cytometry with a diode laser
    Ekaterina I Galanzha
    Phillips Classic Laser and Nanomedicine Laboratories, Department of Otolaryngology Head and Neck Surgery, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA
    Cancer Res 69:7926-34. 2009
    ..These advances make feasible the early diagnosis of melanoma during the initial parallel progression of primary tumor and CTCs, and laser blood purging using noninvasive or hemodialysis-like schematics for the prevention of metastasis...
  38. ncbi request reprint In vivo high-speed imaging of individual cells in fast blood flow
    Vladimir P Zharov
    Philips Classic Laser Biomedical Laboratories, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205 7199, USA
    J Biomed Opt 11:054034. 2006
    ..Potential applications of this technique are discussed with focus on time-resolved monitoring of the cell deformation dynamics in the native biological environment, which may have diagnostic value for the early diagnosis of diseases...
  39. ncbi request reprint Spectral evaluation of laser-induced cell damage with photothermal microscopy
    Dmitri O Lapotko
    Luikov Heat and Mass Transfer Institute, International Research Centre, Minsk, Belarus
    Lasers Surg Med 36:22-30. 2005
    ..This work evaluated the potential of photothermal (PT) technique for studying invasive laser-cell interaction, with a focus on PT evaluation of spectral dependence of laser-induced damage in visible region at single intact cell level...
  40. ncbi request reprint Combination of viral biology and nanotechnology: new applications in nanomedicine
    Vaibhav Saini
    Division of Human Gene Therapy, Department of Medicine, University of Alabama at Birmingham, Birmingham, Alabama 35294 2180, USA
    Nanomedicine 2:200-6. 2006
    ....
  41. ncbi request reprint Photothermal lens detection of gold nanoparticles: theory and experiments
    Anton V Brusnichkin
    M V Lomonosov Moscow State University, Chemistry Department, Vorob evy Hills 1 3, GSP 3 Moscow, Russia
    Appl Spectrosc 61:1191-201. 2007
    ..Further improvements are discussed, including increasing the sensitivity thresholds up to one nanoparticle in the detected volume...
  42. ncbi request reprint Laser-induced explosion of gold nanoparticles: potential role for nanophotothermolysis of cancer
    Renat R Letfullin
    Department of Physics and Optical Engineering, Rose Hulman Institute of Technology, Terre Haute, IN 47803 3999, USA
    Nanomedicine (Lond) 1:473-80. 2006
    ..This article explores the laser-induced explosion of absorbing nanoparticles in selective nanophotothermolysis of cancer...
  43. ncbi request reprint Photothermal time-resolved imaging of living cells
    Dmitri O Lapotko
    Luikov Heat and Mass Transfer Institute, 15 Brovka Street, Minsk, 220072, Belarus
    Lasers Surg Med 31:53-63. 2002
    ..The aim of this study was to evaluate a new method of photothermal imaging (PTI) for monitoring short-time nano-scale thermal effects in individual living cells...

Research Grants11

  1. In vivo real-time detection of circulating melanoma cells
    Vladimir P Zharov; Fiscal Year: 2010
    ....
  2. Photothermal Evaluation of Drug Toxicity at Cell Level
    Vladimir Zharov; Fiscal Year: 2004
    ..abstract_text> ..
  3. Multifunctional nanotubes for in vivo detecting/purging circulating cancer cells
    Vladimir P Zharov; Fiscal Year: 2010
    ....
  4. Multifunctional nanotubes for in vivo detecting/purging circulating cancer cells
    Vladimir Zharov; Fiscal Year: 2009
    ....
  5. In vivo real-time detection of circulating melanoma cells
    Vladimir Zharov; Fiscal Year: 2009
    ....
  6. Integrated Photothermal Nanoprobes
    Vladimir Zharov; Fiscal Year: 2007
    ..The results of these studies will also make it possible to formulate recommendations on the desired properties of new, clustered nanomaterials for medical applications. ..
  7. Photothermal Imaging of Live Cells
    Vladimir Zharov; Fiscal Year: 2007
    ..The technical realization of PTI is relatively simple, requiring the combination of conventional optical phase-contrast microscopy and the already routinely used nanosecond laser. ..
  8. Photothermal Imaging of Live Cells
    Vladimir Zharov; Fiscal Year: 2006
    ..The technical realization of PTI is relatively simple, requiring the combination of conventional optical phase-contrast microscopy and the already routinely used nanosecond laser. ..
  9. Photothermal Imaging of Live Cells
    Vladimir Zharov; Fiscal Year: 2005
    ..The technical realization of PTI is relatively simple, requiring the combination of conventional optical phase-contrast microscopy and the already routinely used nanosecond laser. ..
  10. Integrated optical imaging rat of mesentery
    Vladimir Zharov; Fiscal Year: 2004
    ..The technical realization of this new imaging system is relatively simple, combining transmission microscopy and laser technique already routinely in use. ..