Research Topics
Genomes and Genes | Rui ZhaoSummaryAffiliation: University of Colorado Health Sciences Center Country: USA Publications
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Publications
The leukemia-associated transcription repressor AML1/MDS1/EVI1 requires CtBP to induce abnormal growth and differentiation of murine hematopoietic cellsVitalyi Senyuk
Department of Pathology, The Cancer Center, University of Illinois at Chicago, 900 South Ashland Avenue, Chicago, IL 60607, USA
Oncogene 21:3232-40. 2002....- Mechanisms of conformational change for a replicative hexameric helicase of SV40 large tumor antigenDahai Gai
Department of Biochemistry and Molecular Genetics, University of Colorado Health Sciences Center, Denver, CO 80262, USA
Cell 119:47-60. 2004..Additionally, six unique beta hairpins on the channel surface move longitudinally along the central channel, possibly serving as a motor for pulling DNA into the LTag double hexamer for unwinding... - Crystal structure of UAP56, a DExD/H-box protein involved in pre-mRNA splicing and mRNA exportRui Zhao
Department of Biochemistry and Molecular Genetics, University of Colorado Health Sciences Center, Aurora, Colorado 80045, USA
Structure 12:1373-81. 2004..The crystal structure of the N-terminal domain of UAP56 also reveals a dimer interface that is potentially important for UAP56's function... 
Structural evidence for consecutive Hel308-like modules in the spliceosomal ATPase Brr2Lingdi Zhang
Department of Biochemistry and Molecular Genetics, University of Colorado Denver, Aurora, Colorado, USA
Nat Struct Mol Biol 16:731-9. 2009..We further find that the C-terminal region of Prp8 (Prp8-CTR) facilitates the binding of the Brr2-Prp8-CTR complex to U4/U6. Our results have important implications for the mechanism and regulation of Brr2's activity in splicing...- Structural basis for nematode eIF4E binding an m(2,2,7)G-Cap and its implications for translation initiationWeizhi Liu
Department of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, Aurora, CO 80045, USA
Nucleic Acids Res 39:8820-32. 2011..These data have implications for the contribution of 5'-UTRs in mRNA translation and the function of different eIF4E isoforms... - Biochemical and functional characterization of six SIX1 Branchio-oto-renal syndrome mutationsAaron N Patrick
Program in Molecular Biology, University of Colorado Denver School of Medicine, Aurora, Colorado 80045, USA
J Biol Chem 284:20781-90. 2009.... - Biochemical characterization of the ATPase and helicase activity of UAP56, an essential pre-mRNA splicing and mRNA export factorJingping Shen
Department of Biochemistry and Molecular Genetics, University of Colorado at Denver and Health Sciences Center, Aurora, Colorado 80045, USA
J Biol Chem 282:22544-50. 2007.... - Crystal structure of the beta-finger domain of Prp8 reveals analogy to ribosomal proteinsKui Yang
Department of Biochemistry and Molecular Genetics, Anschutz Medical Campus, University of Colorado Denver, Aurora, CO 80045, USA
Proc Natl Acad Sci U S A 105:13817-22. 2008..These results also demonstrate an analogy between a spliceosomal protein and ribosomal proteins that insert extensions into folded rRNAs and stabilize the ribosome... 
The Hsp90 inhibitor radicicol interacts with the ATP-binding pocket of bacterial sensor kinase PhoQMichael T Guarnieri
Department of Biochemistry and Molecular Genetics, University of Colorado Denver Anschutz Medical Campus, Aurora, CO 80045, USA
J Mol Biol 379:82-93. 2008..Our results suggest that GHL inhibitors may be useful lead compounds for developing sensor kinase inhibitors...- Structural insights into parasite eIF4E binding specificity for m7G and m2,2,7G mRNA capsWeizhi Liu
Department of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, Aurora, Colorado 80045, USA
J Biol Chem 284:31336-49. 2009..Overall the NMR and isothermal titration calorimetry data suggest the importance of intrinsic conformational flexibility in the schistosome eIF4E that enables binding to m(2,2,7)G cap... - Molecular mechanism of histone H3K4me3 recognition by plant homeodomain of ING2Pedro V Peña
Department of Pharmacology, University of Colorado Health Sciences Center, Aurora, Colorado 80045, USA
Nature 442:100-3. 2006..Elucidation of the mechanisms underlying this novel function of PHD fingers provides a basis for deciphering the role of the ING family of tumour suppressors in chromatin regulation and signalling... - Crystal structure of the C-terminal domain of splicing factor Prp8 carrying retinitis pigmentosa mutantsLingdi Zhang
Department of Biochemistry and Molecular Genetics, University of Colorado at Denver and Health Sciences Center, Aurora, Colorado 80045, USA
Protein Sci 16:1024-31. 2007..Mapping of RP13 mutants on the Prp8 structure suggests that these residues constitute a binding surface between Prp8 and other partner(s), and the disruption of this interaction provides a plausible molecular mechanism for RP13... - Structure of a specific alcohol-binding site defined by the odorant binding protein LUSH from Drosophila melanogasterSchoen W Kruse
Department of Pharmacology, University of Colorado Health Sciences Center, M S C236, 4200 East Ninth Avenue, Denver, Colorado 80262, USA
Nat Struct Biol 10:694-700. 2003..Further, these sequences are found in regions of ion channels that are known to confer alcohol sensitivity. We suggest that the alcohol-binding site in LUSH represents a general model for alcohol-binding sites in proteins... - A high-throughput TNP-ATP displacement assay for screening inhibitors of ATP-binding in bacterial histidine kinasesMichael T Guarnieri
Department of Biochemistry and Molecular Genetics, University of Colorado Denver, Aurora, 80045, USA
Assay Drug Dev Technol 9:174-83. 2011..We utilized the assay to screen a library of GHL inhibitors targeting the bacterial HK PhoQ, and discuss the applications of the 2',3'-O-(2,4,6-trinitrophenyl) adenosine 5'-triphosphate competition assay beyond GHKL inhibitor screening... - Structure of the replicative helicase of the oncoprotein SV40 large tumour antigenDawei Li
Department of Biochemistry and Molecular Genetics, University of Colorado Health Science Center, School of Medicine, Denver, Colorado 80262, USA
Nature 423:512-8. 2003.... - Transcriptional down-regulation of Brca1 and E-cadherin by CtBP1 in breast cancerYu Deng
Department of Dermatology, University of Colorado, Denver, Aurora, Colorado 80045, USA
Mol Carcinog 51:500-7. 2012..Thus, CtBP1 activation could be a potential biomarker for breast cancer development... - Identification of a selective small-molecule inhibitor series targeting the eyes absent 2 (eya2) phosphatase activityAaron B Krueger
1Department of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, Aurora, CO, USA
J Biomol Screen 18:85-96. 2013..Our studies identify the first selective Eya2 phosphatase inhibitors that can potentially be developed into chemical probes for functional studies of Eya phosphatase or into anticancer drugs in the future... - Distinct activities of the DExD/H-box splicing factor hUAP56 facilitate stepwise assembly of the spliceosomeHaihong Shen
Department of Life Science, Gwangju Institute of Science and Technology, Gwangju 500 712, Korea
Genes Dev 22:1796-803. 2008..Our results indicate that hUAP56 first interacts with U2AF(65) in an ATP-dependent manner, and subsequently with U4/U6 snRNAs to facilitate stepwise assembly of the spliceosome... - Determining binding sites of drugs on human serum albumin using FIA-QCMQundan Zhang
Beijing National Laboratory for Molecular Sciences, Laboratory of Analytical Chemistry for Life Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing, China
Biosens Bioelectron 24:48-54. 2008..The results demonstrate FIA-QCM can be a valid approach for monitoring the dynamic interaction between drugs and HSA in real time further identifying drug-binding sites without the need of labels... - Crystal structures of four types of human papillomavirus L1 capsid proteins: understanding the specificity of neutralizing monoclonal antibodiesBrooke Bishop
Department of Molecular and Computational Biology, University of Southern California, Los Angeles, California 90089, USA
J Biol Chem 282:31803-11. 2007..On the basis of these distinct surface loop structures, we have provided a molecular explanation for the type specificity of NmAbs against HPV infection... - CheZ-mediated dephosphorylation of the Escherichia coli chemotaxis response regulator CheY: role for CheY glutamate 89Ruth E Silversmith
Department of Microbiology and Immunology, University of North Carolina, Chapel Hill, North Carolina 27599, USA
J Bacteriol 185:1495-502. 2003..The CheZ resistance of CheYN59R can thus be largely explained by the formation of the salt bridge between Arg59 and Glu89, which prevents Glu89 from executing its role in catalysis... - Structure and catalytic mechanism of the E. coli chemotaxis phosphatase CheZRui Zhao
Department of Microbiology and Immunology, University of North Carolina, Chapel Hill, North Carolina, 27599 7290 USA
Nat Struct Biol 9:570-5. 2002..Similarities between the CheY[bond] CheZ and Spo0F [bond]Spo0B structures suggest a general mode of interaction for modulation of response regulator phosphorylation chemistry... - Direct mass spectrometric analysis of intact proteins of the yeast large ribosomal subunit using capillary LC/FTICRSang-Won Lee
Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, P.O. Box 999, Richland, WA 99352, USA
Proc Natl Acad Sci U S A 99:5942-7. 2002....
Research Grants
- Understanding the structure and function of splicing factor Prp8Rui Zhao; Fiscal Year: 2010..This approach is a critical step toward generating a complete picture of Prp8 that cannot be obtained otherwise, significantly advancing our understanding of the molecular mechanisms of pre-mRNA splicing. ..
- Understanding the structure and function of splicing factor Prp8Rui Zhao; Fiscal Year: 2009..This approach is a critical step toward generating a complete picture of Prp8 that cannot be obtained otherwise, significantly advancing our understanding of the molecular mechanisms of pre-mRNA splicing. ..