Yi Zhang

Summary

Affiliation: University of North Carolina
Country: USA

Publications

  1. ncbi request reprint Transcription regulation by histone methylation: interplay between different covalent modifications of the core histone tails
    Y Zhang
    Department of Biochemistry and Biophysics, Curriculum in Genetics and Molecular Biology, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, North Carolina 27599 7295, USA
    Genes Dev 15:2343-60. 2001
  2. ncbi request reprint Transcriptional regulation by histone ubiquitination and deubiquitination
    Yi Zhang
    Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, North Carolina 27599, USA
    Genes Dev 17:2733-40. 2003
  3. pmc The H3K4 demethylase lid associates with and inhibits histone deacetylase Rpd3
    Nara Lee
    Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7295
    Mol Cell Biol 29:1401-10. 2009
  4. ncbi request reprint Role of Bmi-1 and Ring1A in H2A ubiquitylation and Hox gene silencing
    Ru Cao
    Howard Hughes Medical Institute, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, 27599, USA
    Mol Cell 20:845-54. 2005
  5. ncbi request reprint mAM facilitates conversion by ESET of dimethyl to trimethyl lysine 9 of histone H3 to cause transcriptional repression
    Hengbin Wang
    Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Mol Cell 12:475-87. 2003
  6. ncbi request reprint Developmental regulation of Suz 12 localization
    Cecile C de la Cruz
    Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94143, USA
    Chromosoma 114:183-92. 2005
  7. ncbi request reprint SUZ12 is required for both the histone methyltransferase activity and the silencing function of the EED-EZH2 complex
    Ru Cao
    Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, Curriculum in Genetics and Molecular Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Mol Cell 15:57-67. 2004
  8. ncbi request reprint Role of histone H3 lysine 27 methylation in Polycomb-group silencing
    Ru Cao
    Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7295, USA
    Science 298:1039-43. 2002
  9. ncbi request reprint Substrate preferences of the EZH2 histone methyltransferase complex
    Cyrus Martin
    Howard Hughes Medical Institute, Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, North Carolina 27599, USA
    J Biol Chem 281:8365-70. 2006
  10. pmc Role of hPHF1 in H3K27 methylation and Hox gene silencing
    Ru Cao
    Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7295, USA
    Mol Cell Biol 28:1862-72. 2008

Collaborators

Detail Information

Publications105 found, 100 shown here

  1. ncbi request reprint Transcription regulation by histone methylation: interplay between different covalent modifications of the core histone tails
    Y Zhang
    Department of Biochemistry and Biophysics, Curriculum in Genetics and Molecular Biology, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, North Carolina 27599 7295, USA
    Genes Dev 15:2343-60. 2001
  2. ncbi request reprint Transcriptional regulation by histone ubiquitination and deubiquitination
    Yi Zhang
    Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, North Carolina 27599, USA
    Genes Dev 17:2733-40. 2003
  3. pmc The H3K4 demethylase lid associates with and inhibits histone deacetylase Rpd3
    Nara Lee
    Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7295
    Mol Cell Biol 29:1401-10. 2009
    ..Thus, our finding that Lid antagonizes Rpd3 function provides an explanation for the genetic classification of Lid as a positive transcription regulator...
  4. ncbi request reprint Role of Bmi-1 and Ring1A in H2A ubiquitylation and Hox gene silencing
    Ru Cao
    Howard Hughes Medical Institute, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, 27599, USA
    Mol Cell 20:845-54. 2005
    ..Our results suggest that EZH2-mediated H3-K27 methylation functions upstream of PRC1 and establishes a critical role for Bmi-1 and Ring1A in H2A ubiquitylation and Hox gene silencing...
  5. ncbi request reprint mAM facilitates conversion by ESET of dimethyl to trimethyl lysine 9 of histone H3 to cause transcriptional repression
    Hengbin Wang
    Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Mol Cell 12:475-87. 2003
    ..Thus, our studies establish that promoter H3-K9 trimethylation is the cause of transcriptional repression and that mAM/hAM facilitates conversion of H3-K9 dimethyl to trimethyl by ESET/SETDB1...
  6. ncbi request reprint Developmental regulation of Suz 12 localization
    Cecile C de la Cruz
    Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94143, USA
    Chromosoma 114:183-92. 2005
    ..These results suggest that Suz12 may have a function that is not mediated by its association with Eed and Ezh2, and that this additional function is not involved in the regulation of X-inactivation...
  7. ncbi request reprint SUZ12 is required for both the histone methyltransferase activity and the silencing function of the EED-EZH2 complex
    Ru Cao
    Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, Curriculum in Genetics and Molecular Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Mol Cell 15:57-67. 2004
    ..Thus, our study establishes a critical role of SUZ12 in H3-lysine 27 methylation and Hox gene silencing...
  8. ncbi request reprint Role of histone H3 lysine 27 methylation in Polycomb-group silencing
    Ru Cao
    Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7295, USA
    Science 298:1039-43. 2002
    ..Methylation on H3-K27 facilitates binding of Polycomb (PC), a component of the PRC1 complex, to histone H3 amino-terminal tail. Thus, these studies establish a link between histone methylation and PcG-mediated gene silencing...
  9. ncbi request reprint Substrate preferences of the EZH2 histone methyltransferase complex
    Cyrus Martin
    Howard Hughes Medical Institute, Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, North Carolina 27599, USA
    J Biol Chem 281:8365-70. 2006
    ..Our work supports the hypothesis that linker histone H1 and chromatin structure are important factors in determining the substrate preference of the EZH2 histone methyltransferase complex...
  10. pmc Role of hPHF1 in H3K27 methylation and Hox gene silencing
    Ru Cao
    Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7295, USA
    Mol Cell Biol 28:1862-72. 2008
    ..Therefore, our results reveal hPHF1 as a component of a novel EED-EZH2 complex and demonstrate its important role in H3K27 methylation and Hox gene silencing...
  11. ncbi request reprint The functions of E(Z)/EZH2-mediated methylation of lysine 27 in histone H3
    Ru Cao
    Department of Biochemistry and Biophysics, Curriculum in Genetics and Molecular Biology, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, NC 27599 7295, USA
    Curr Opin Genet Dev 14:155-64. 2004
    ....
  12. ncbi request reprint Role of histone H2A ubiquitination in Polycomb silencing
    Hengbin Wang
    Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 7295, USA
    Nature 431:873-8. 2004
    ..Thus, our studies identify the H2A ubiquitin ligase, and link H2A ubiquitination to Polycomb silencing...
  13. ncbi request reprint The transcriptional repressor JHDM3A demethylates trimethyl histone H3 lysine 9 and lysine 36
    Robert J Klose
    Howard Hughes Medical Institute, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 7295, USA
    Nature 442:312-6. 2006
    ....
  14. pmc The Saccharomyces cerevisiae histone demethylase Jhd1 fine-tunes the distribution of H3K36me2
    Jia Fang
    Howard Hughes Medical Institute, University of North Carolina at Chapel Hill, NC 27599, USA
    Mol Cell Biol 27:5055-65. 2007
    ..Our studies establish Jhd1 as a histone demethylase in budding yeast and suggest that Jhd1 functions to maintain the fidelity of histone methylation patterns along transcription units...
  15. pmc pRB family proteins are required for H3K27 trimethylation and Polycomb repression complexes binding to and silencing p16INK4alpha tumor suppressor gene
    Yojiro Kotake
    Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, Program in Molecular Biology and Biotechnology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Genes Dev 21:49-54. 2007
    ..These results suggest a model in which pRB proteins recruit PRC2 to trimethylate p16, priming the BMI1-containing PRC1L ubiquitin ligase complex to silence p16...
  16. ncbi request reprint Histone demethylation by a family of JmjC domain-containing proteins
    Yu Ichi Tsukada
    Howard Hughes Medical Institute, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 7295, USA
    Nature 439:811-6. 2006
    ..Thus, we identify the JmjC domain as a novel demethylase signature motif and uncover a protein demethylation mechanism that is conserved from yeast to human...
  17. pmc Demethylation of histone H3K36 and H3K9 by Rph1: a vestige of an H3K9 methylation system in Saccharomyces cerevisiae?
    Robert J Klose
    Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7295, USA
    Mol Cell Biol 27:3951-61. 2007
    ..The capacity of Rph1 to demethylate H3K9 provides the first indication that S. cerevisiae may have once encoded an H3K9 methylation system and suggests that Rph1 is a functional vestige of this modification system...
  18. ncbi request reprint JmjC-domain-containing proteins and histone demethylation
    Robert J Klose
    Howard Hughes Medical Institute, Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, North Carolina 27599 7295, USA
    Nat Rev Genet 7:715-27. 2006
    ..Here we analyse the evolutionary relationship between Jumonji C (JmjC)-domain-containing proteins and discuss their cellular functions in relation to their potential enzymatic activities...
  19. ncbi request reprint The retinoblastoma binding protein RBP2 is an H3K4 demethylase
    Robert J Klose
    Howard Hughes Medical Institute, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Cell 128:889-900. 2007
    ..These studies provide mechanistic insights into transcriptional regulation by RBP2 and provide the first example of a mammalian enzyme capable of erasing trimethylated H3K4...
  20. ncbi request reprint hDOT1L links histone methylation to leukemogenesis
    Yuki Okada
    Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Cell 121:167-78. 2005
    ..Our studies thus establish that mistargeting of hDOT1L to Hoxa9 plays an important role in MLL-AF10-mediated leukemogenesis and suggests that the enzymatic activity of hDOT1L may provide a potential target for therapeutic intervention...
  21. pmc The histone methyltransferase Set7/9 promotes myoblast differentiation and myofibril assembly
    Yazhong Tao
    McAllister Heart Institute, University of North Carolina, Chapel Hill, NC 27599, USA
    J Cell Biol 194:551-65. 2011
    ..Together, our experiments define a biological function for Set7 in muscle differentiation and provide a molecular mechanism by which Set7 modulates myogenic transcription factors during muscle differentiation...
  22. ncbi request reprint PLU-1 is an H3K4 demethylase involved in transcriptional repression and breast cancer cell proliferation
    Kenichi Yamane
    Howard Hughes Medical Institute, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7295, USA
    Mol Cell 25:801-12. 2007
    ..Furthermore, we reveal that PLU-1-mediated H3K4 demethylase activity plays an important role in the proliferative capacity of breast cancer cells through repression of tumor suppressor genes, including BRCA1...
  23. ncbi request reprint Purification and functional characterization of SET8, a nucleosomal histone H4-lysine 20-specific methyltransferase
    Jia Fang
    Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, 27599, USA
    Curr Biol 12:1086-99. 2002
    ..Thus far, only 2 residues on histone H4 are known to be methylated. While H4-arginine 3 (H4-R3) methylation is mediated by PRMT1, the enzyme(s) responsible for H4-lysine 20 (H4-K20) methylation is not known...
  24. pmc The H3K36 demethylase Jhdm1b/Kdm2b regulates cell proliferation and senescence through p15(Ink4b)
    Jin He
    Howard Hughes Medical Institute, Chapel Hill, North Carolina 27599 7295, USA
    Nat Struct Mol Biol 15:1169-75. 2008
    ..Alteration of Jhdm1b level affects Ras-induced neoplastic transformation. Collectively, our results indicate that Jhdm1b is an H3K36 demethylase that regulates cell proliferation and senescence through p15(Ink4b)...
  25. ncbi request reprint JHDM2A, a JmjC-containing H3K9 demethylase, facilitates transcription activation by androgen receptor
    Kenichi Yamane
    Howard Hughes Medical Institute, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Cell 125:483-95. 2006
    ..Thus, our work identifies a histone demethylase and links its function to hormone-dependent transcriptional activation...
  26. ncbi request reprint Methylation of H3-lysine 79 is mediated by a new family of HMTases without a SET domain
    Qin Feng
    Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, 27599, USA
    Curr Biol 12:1052-8. 2002
    ..Furthermore, we found that K79 methylation level is regulated throughout the cell cycle. Thus, our studies reveal a new methylation site and define a novel family of histone lysine methyltransferase...
  27. ncbi request reprint Ring1b-mediated H2A ubiquitination associates with inactive X chromosomes and is involved in initiation of X inactivation
    Jia Fang
    Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7295, USA
    J Biol Chem 279:52812-5. 2004
    ..Furthermore, we showed that the association of Ring1b and ubH2A with Xi is mitotically stable in non-differentiated TS cells...
  28. pmc Genome-wide uH2A localization analysis highlights Bmi1-dependent deposition of the mark at repressed genes
    Eric M Kallin
    Howard Hughes Medical Institute, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA
    PLoS Genet 5:e1000506. 2009
    ..Thus, our work not only reveals Bmi1-dependent H2A ubiquitylation, but also suggests that uH2A targeting in differentiated cells may employ a different mechanism from that in ES cells...
  29. pmc Inactivation of NuRD component Mta2 causes abnormal T cell activation and lupus-like autoimmune disease in mice
    Xiangdong Lu
    Howard Hughes Medical Institute, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    J Biol Chem 283:13825-33. 2008
    ..Our study suggests that Mta2/NuRD is involved in modulating IL-4 and IFN-gamma expression in T cell immune responses, and gene expression in non-T cells plays an important role in controlling autoimmunity...
  30. doi request reprint Role of Jhdm2a in regulating metabolic gene expression and obesity resistance
    Keisuke Tateishi
    Howard Hughes Medical Institute, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 7295, USA
    Nature 458:757-61. 2009
    ..Our studies thus demonstrate an essential role for Jhdm2a in regulating metabolic gene expression and normal weight control in mice...
  31. pmc DOT1L, the H3K79 methyltransferase, is required for MLL-AF9-mediated leukemogenesis
    Anh Tram Nguyen
    Howard Hughes Medical Institute, Chapel Hill, NC, USA
    Blood 117:6912-22. 2011
    ..Thus, DOT1L may serve as a potential therapeutic target for the treatment of leukemia caused by MLL translocations...
  32. ncbi request reprint Purification of histone methyltransferases from HeLa cells
    Jia Fang
    Lineberger Comprehensive Cancer Center, Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 7295, USA
    Methods Enzymol 377:213-26. 2004
  33. ncbi request reprint Role of histone H3 lysine 27 methylation in X inactivation
    Kathrin Plath
    Department of Biochemistry and Biophysics, University of California San Francisco, San Francisco, CA 94143, USA
    Science 300:131-5. 2003
    ..Together, our results suggest a role for Eed-Ezh2-mediated H3-K27 methylation during initiation of both imprinted and random X inactivation and demonstrate that H3-K27 methylation is not sufficient for silencing of the Xi...
  34. ncbi request reprint Leukaemic transformation by CALM-AF10 involves upregulation of Hoxa5 by hDOT1L
    Yuki Okada
    Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7295, USA
    Nat Cell Biol 8:1017-24. 2006
    ..Thus, our study establishes CALM-AF10 fusion as a cause of leukaemia and reveals that mistargeting of hDOT1L and upregulation of Hoxa5 through H3K79 methylation is the underlying mechanism behind leukaemia caused by CALM-AF10 fusion...
  35. pmc Generation and replication-dependent dilution of 5fC and 5caC during mouse preimplantation development
    Azusa Inoue
    Howard Hughes Medical Institute, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Cell Res 21:1670-6. 2011
    ..Together with previous studies, our study suggests that Tet-catalyzed conversion of 5mC to 5hmC/5fC/5caC followed by replication-dependent dilution accounts for paternal DNA demethylation during preimplantation development...
  36. ncbi request reprint Histone demethylase JHDM2A is critical for Tnp1 and Prm1 transcription and spermatogenesis
    Yuki Okada
    Howard Hughes Medical Institute, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 7295, USA
    Nature 450:119-23. 2007
    ..Thus, our work uncovers a role for JHDM2A in spermatogenesis and reveals transition nuclear protein and protamine genes as direct targets of JHDM2A...
  37. ncbi request reprint The diverse functions of histone lysine methylation
    Cyrus Martin
    Howard Hughes Medical Institute, Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, North Carolina 27599 7295, USA
    Nat Rev Mol Cell Biol 6:838-49. 2005
    ..Here, we summarize recent advances in our understanding of how lysine methylation functions in these diverse biological processes, and raise questions that need to be addressed in the future...
  38. ncbi request reprint Mechanisms of epigenetic inheritance
    Cyrus Martin
    Howard Hughes Medical Institute, Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, NC 27599 7295, USA
    Curr Opin Cell Biol 19:266-72. 2007
    ..Finally, several recent studies suggest that the faithful replication of DNA methylation patterns may be used to propagate histone modifications associated with gene silencing...
  39. ncbi request reprint Regulation of histone methylation by demethylimination and demethylation
    Robert J Klose
    Howard Hughes Medical Institute, Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 7295, USA
    Nat Rev Mol Cell Biol 8:307-18. 2007
    ..Here, we examine the enzymatic and structural basis for the mechanisms that these enzymes use to counteract histone methylation and provide insights into their substrate specificity and biological function...
  40. pmc Minireview: role of protein methylation and demethylation in nuclear hormone signaling
    Susan C Wu
    Howard Hughes Medical Institute, Department of Biochemistry, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 7295, USA
    Mol Endocrinol 23:1323-34. 2009
    ..This review discusses recent progress in our understanding of the role of methylation and demethylation of histones, NRs, and their coregulators in NR-mediated transcription...
  41. doi request reprint Histone demethylase JHDM2A is involved in male infertility and obesity
    Yuki Okada
    Howard Hughes Medical Institute, Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA
    J Androl 31:75-8. 2010
    ..Thus, JHDM2A possesses organ/tissue-specific target genes, and impairment of this molecule cannot be compensated by other JmjC-containing histone demethylases, suggesting the importance of this molecule in vivo...
  42. pmc Cyclin-dependent kinase 1 (CDK1)-mediated phosphorylation of enhancer of zeste 2 (Ezh2) regulates its stability
    Susan C Wu
    Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, Howard Hughes Medical Institute, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    J Biol Chem 286:28511-9. 2011
    ..Collectively, these results establish a novel function for CDK1-mediated Ezh2 phosphorylation and provide a mechanism by which Ezh2 protein levels can be regulated in cells...
  43. ncbi request reprint Yeast Jhd2p is a histone H3 Lys4 trimethyl demethylase
    Gaoyang Liang
    Howard Hughes Medical Institute, Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 7295, USA
    Nat Struct Mol Biol 14:243-5. 2007
    ..Here we identify a novel budding yeast JmjC domain-containing H3-K4 demethylase, Jhd2p, that antagonizes the trimethyl modification state and contributes to regulation of telomeric silencing...
  44. doi request reprint Generation of insulin-secreting islet-like clusters from human skin fibroblasts
    Keisuke Tateishi
    Howard Hughes Medical Institute, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 7295, USA
    J Biol Chem 283:31601-7. 2008
    ..Thus, our study provides evidence that insulin-secreting ILCs can be generated from skin fibroblasts, raising the possibility that patient-specific iPS cells could potentially provide a treatment for diabetes in the future...
  45. pmc Purification of histone demethylases from HeLa cells
    Yu Ichi Tsukada
    Howard Hughes Medical Institute, Chapel Hill, NC 27599 7295, USA
    Methods 40:318-26. 2006
    ..Here, we describe assays currently used for measuring histone demethylase activity and chromatography strategies used in purifying histone demethylases from HeLa cells...
  46. pmc Active DNA demethylation: many roads lead to Rome
    Susan C Wu
    Howard Hughes Medical Institute, and Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 7295, USA
    Nat Rev Mol Cell Biol 11:607-20. 2010
    ..Insight into how DNA methylation is dynamically regulated will broaden our understanding of epigenetic regulation and have great implications in somatic cell reprogramming and regenerative medicine...
  47. pmc Role of H3K27 methylation in the regulation of lncRNA expression
    Susan C Wu
    Howard Hughes Medical Institute, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7295, USA
    Cell Res 20:1109-16. 2010
    ..Thus, our results establish a role for Ezh2-mediated H3K27 methylation in lncRNA silencing in ES cells and reveal that lncRNAs are subject to epigenetic regulation in a similar manner to that of the protein-coding genes...
  48. pmc Kdm2b promotes induced pluripotent stem cell generation by facilitating gene activation early in reprogramming
    Gaoyang Liang
    Howard Hughes Medical Institute, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 7295, USA
    Nat Cell Biol 14:457-66. 2012
    ..Our studies not only identify an important epigenetic factor for iPSC generation, but also reveal the molecular mechanism underlying how Kdm2b contributes to reprogramming...
  49. pmc Identification and functional characterization of the p66/p68 components of the MeCP1 complex
    Qin Feng
    Department of Biochemistry and Biophysics, Curriculum in Genetics and Molecular Biology, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 7295, USA
    Mol Cell Biol 22:536-46. 2002
    ..The identification and characterization of all the MeCP1 components set the stage for reconstitution of the MeCP1 complex...
  50. pmc Replication-dependent loss of 5-hydroxymethylcytosine in mouse preimplantation embryos
    Azusa Inoue
    Howard Hughes Medical Institute HHMI, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Science 334:194. 2011
    ..Our study suggests that, although the conversion of 5mC to 5hmC in zygotes is an enzyme-catalyzed process, loss of 5hmC during preimplantation appears to be a DNA replication-dependent passive process...
  51. pmc A role for the elongator complex in zygotic paternal genome demethylation
    Yuki Okada
    Howard Hughes Medical Institute, Chapel Hill, North Carolina 27599 7295, USA
    Nature 463:554-8. 2010
    ....
  52. pmc KDM2b/JHDM1b, an H3K36me2-specific demethylase, is required for initiation and maintenance of acute myeloid leukemia
    Jin He
    Howard Hughes Medical Institute, and Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, NC 27599, USA
    Blood 117:3869-80. 2011
    ..Thus, our study suggests that Kdm2b/Jhdm1b functions as an oncogene and plays a critical role in leukemia development and maintenance...
  53. pmc DOT1L regulates dystrophin expression and is critical for cardiac function
    Anh T Nguyen
    Howard Hughes Medical Institute, University of North Carolina at Chapel Hill, NC 27599, USA
    Genes Dev 25:263-74. 2011
    ..In addition, our study may open new avenues for the diagnosis and treatment of human heart disease...
  54. doi request reprint Janus kinase 2: an epigenetic 'writer' that activates leukemogenic genes
    Jin He
    Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, Howard Hughes Medical Institute, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7295, USA
    J Mol Cell Biol 2:231-3. 2010
    ..Nature 461, 819-822) report that JAK2 performs this function by displacing the heterochromatin protein HP1╬▒ from chromatin through phosphorylation of histone H3...
  55. pmc Enzymatic analysis of Tet proteins: key enzymes in the metabolism of DNA methylation
    Li Shen
    Howard Hughes Medical Institute Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA
    Methods Enzymol 512:93-105. 2012
    ..Here, we describe 2D-TLC and mass spectrometry methods that we have successfully used in differentiating 5mC from its oxidative derivatives as well as in characterizing the enzymatic activity of Tet proteins both in vitro and in vivo...
  56. pmc Butyrate promotes induced pluripotent stem cell generation
    Gaoyang Liang
    Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, Howard Hughes Medical Institute, University of North Carolina, Chapel Hill, North Carolina 27599 7295, USA
    J Biol Chem 285:25516-21. 2010
    ..Thus, our study identifies butyrate as a chemical factor capable of promoting iPS cell generation...
  57. pmc Tet proteins can convert 5-methylcytosine to 5-formylcytosine and 5-carboxylcytosine
    Shinsuke Ito
    Howard Hughes Medical Institute and Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7295, USA
    Science 333:1300-3. 2011
    ..Thus, we identify two previously unknown cytosine derivatives in genomic DNA as the products of Tet proteins. Our study raises the possibility that DNA demethylation may occur through Tet-catalyzed oxidation followed by decarboxylation...
  58. pmc Mitochondrial network size scaling in budding yeast
    Susanne M Rafelski
    Department of Biochemistry and Biophysics, University of California San Francisco UCSF, San Francisco, CA, USA
    Science 338:822-4. 2012
    ..Thus, yeast populations achieve a stable scaling relation between mitochondrial content and cell size despite asymmetry in inheritance...
  59. ncbi request reprint The NuRD complex: linking histone modification to nucleosome remodeling
    Q Feng
    Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, NC 27599 7295, USA
    Curr Top Microbiol Immunol 274:269-90. 2003
    ..In addition, it may also participate in genome-wide transcriptional regulation through an association with histone tails...
  60. ncbi request reprint Sp1 and ETS family transcription factors regulate the mouse Mta2 gene expression
    L Xia
    Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7295, USA
    Gene 268:77-85. 2001
    ....
  61. pmc The MeCP1 complex represses transcription through preferential binding, remodeling, and deacetylating methylated nucleosomes
    Q Feng
    Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, North Carolina 27599 7295, USA
    Genes Dev 15:827-32. 2001
    ..Thus, our study defines the MeCP1 complex, and provides biochemical evidence linking nucleosome remodeling and histone deacetylation to methylated gene silencing...
  62. ncbi request reprint Mutations in human ARF exon 2 disrupt its nucleolar localization and impair its ability to block nuclear export of MDM2 and p53
    Y Zhang
    Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill 27599, USA
    Mol Cell 3:579-91. 1999
    ..Our results suggest an ARF-regulated MDM2-dependent p53 stabilization and link the human tumor-associated mutations in ARF with a functional alteration...
  63. ncbi request reprint Mechanism of Polycomb group gene silencing
    Y Zhang
    Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 7295, USA
    Cold Spring Harb Symp Quant Biol 69:309-17. 2004
  64. pmc The CUL1 C-terminal sequence and ROC1 are required for efficient nuclear accumulation, NEDD8 modification, and ubiquitin ligase activity of CUL1
    M Furukawa
    Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 7295, USA
    Mol Cell Biol 20:8185-97. 2000
    ..This pathway may be commonly utilized for the assembly of other cullin ligases...
  65. ncbi request reprint The cytotoxicity of 1-(1-phenylalkylideneamino)-2,4-azetidinediones and their mode of action in human and murine tumor cells
    R A Izydore
    Division of Medicinal Chemistry and Natural Products, School of Pharmacy, University of North Carolina, Chapel Hill, North Carolina, 27599-7360, USA
    Anticancer Res 21:157-65. 2001
    ..The DNA molecule itself was not a target for the agents in that alkylation of nucleoide bases, intercalation between base pairs, and cross-linking of DNA strands did not occur...
  66. ncbi request reprint Methylation of histone H4 at arginine 3 facilitating transcriptional activation by nuclear hormone receptor
    H Wang
    Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7295, USA
    Science 293:853-7. 2001
    ..Our finding reveals Arg 3 of H4 as a novel methylation site by PRMT1 and indicates that Arg 3 methylation plays an important role in transcriptional regulation...
  67. pmc Mi2, an auto-antigen for dermatomyositis, is an ATP-dependent nucleosome remodeling factor
    H B Wang
    Department of Biochemistry and Biophysics, Curriculum in Genetics and Molecular Biology, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7295, USA
    Nucleic Acids Res 29:2517-21. 2001
    ..Our results define the biochemical function of Mi2 and set the stage for understanding the mechanism of nucleosome remodeling in a defined reconstituted system...
  68. ncbi request reprint Purification and functional characterization of a histone H3-lysine 4-specific methyltransferase
    H Wang
    Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Mol Cell 8:1207-17. 2001
    ..Thus, our study provides a molecular explanation to the differential effects of H3-K4 and H3-K9 methylation on transcription...
  69. doi request reprint Chemical answers to epigenetic crosstalk
    Nara Lee
    Nat Chem Biol 4:335-7. 2008
  70. ncbi request reprint Histone H3 Arg2 methylation provides alternative directions for COMPASS
    Robert J Klose
    Nat Struct Mol Biol 14:1058-60. 2007
  71. ncbi request reprint Molecular cloning of ESET, a novel histone H3-specific methyltransferase that interacts with ERG transcription factor
    Liu Yang
    Medical Research Service, VA Puget Sound Health Care System, University of Washington, Seattle, Washington, WA 98108, USA
    Oncogene 21:148-52. 2002
    ..Together, these findings raise the possibility that transcription factor ERG may participate in transcriptional regulation through ESET-mediated histone methylation...
  72. ncbi request reprint The C. elegans methionine aminopeptidase 2 analog map-2 is required for germ cell proliferation
    Mike Boxem
    Massachusetts General Hospital Cancer Center, Charlestown, MA 02129, USA
    FEBS Lett 576:245-50. 2004
    ..These observations suggest that MAP-2 is essential for germ cell development in C. elegans and that this ubiquitous enzyme may play important roles in a tissue specific manner...
  73. ncbi request reprint Molecular biology: no exception to reversibility
    Yi Zhang
    Nature 431:637-9. 2004
  74. ncbi request reprint The MES-2/MES-3/MES-6 complex and regulation of histone H3 methylation in C. elegans
    Laurel B Bender
    Department of Biology, Indiana University, Bloomington, IN 47405, USA
    Curr Biol 14:1639-43. 2004
    ..Our results contribute to understanding the composition and roles of E(Z)/MES-2 complexes across species...
  75. ncbi request reprint Hierarchical recruitment of polycomb group silencing complexes
    Liangjun Wang
    Department of Biological Sciences, Southern Methodist University, Dallas, TX 75275, USA
    Mol Cell 14:637-46. 2004
    ....
  76. ncbi request reprint Imprinting along the Kcnq1 domain on mouse chromosome 7 involves repressive histone methylation and recruitment of Polycomb group complexes
    David Umlauf
    Institute of Molecular Genetics, CNRS UMR 5535 and University of Montpellier II, 1919, route de Mende, 34090 Montpellier, France
    Nat Genet 36:1296-300. 2004
    ....
  77. ncbi request reprint The in vitro reconstitution of nucleosome and its binding patterns with HMG1/2 and HMG14/17 proteins
    Shu Bing Zhang
    Group of Globin Gene Expression and Regulation, State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China
    Cell Res 13:351-9. 2003
    ....
  78. ncbi request reprint Identification of alternate splice variants for murine SmX5
    Yi Zhang
    Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
    Yi Chuan Xue Bao 30:515-20. 2003
    ....
  79. ncbi request reprint Dynamic regulation of histone H3 methylated at lysine 79 within a tissue-specific chromatin domain
    Hogune Im
    Molecular and Cellular Pharmacology Program, Department of Pharmacology, University of Wisconsin Medical School, Madison, Wisconsin 53706, USA
    J Biol Chem 278:18346-52. 2003
    ..As H3-meK79 is highly regulated at the beta-globin locus, we propose that the murine ortholog of Disruptor of Telomeric Silencing-1-like (mDOT1L) methyltransferase, which synthesizes H3-meK79, regulates beta-globin transcription...
  80. ncbi request reprint Bre1, an E3 ubiquitin ligase required for recruitment and substrate selection of Rad6 at a promoter
    Adam Wood
    Department of Biochemistry, Saint Louis University School of Medicine, 1402 South Grand Boulevard, St Louis, MO 63104, USA
    Mol Cell 11:267-74. 2003
    ..These results suggest that Bre1 is the likely E3 enzyme that directs Rad6 to modify chromatin and ultimately to affect gene expression...
  81. ncbi request reprint Ubiquitination of histone H2B by Rad6 is required for efficient Dot1-mediated methylation of histone H3 lysine 79
    Huck Hui Ng
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 277:34655-7. 2002
    ..Our study provides a new example of trans-histone regulation between modifications on different histones. In addition, it suggests that Rad6 affects telomeric silencing, at least in part, by influencing methylation of histone H3...
  82. pmc Lysine methylation within the globular domain of histone H3 by Dot1 is important for telomeric silencing and Sir protein association
    Huck Hui Ng
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Genes Dev 16:1518-27. 2002
    ..Our results indicate that histone modifications in the core globular domain have important biological functions...
  83. ncbi request reprint Melanocortin activation of nucleus of the solitary tract avoids anorectic tachyphylaxis and induces prolonged weight loss
    Gang Li
    Department of Pharmacology and Therapeutics, University of Florida College of Medicine, Gainesville, Florida 32610, USA
    Am J Physiol Endocrinol Metab 293:E252-8. 2007
    ....
  84. pmc Structural basis for specific binding of Polycomb chromodomain to histone H3 methylated at Lys 27
    Jinrong Min
    W M Keck Structural Biology Laboratory, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
    Genes Dev 17:1823-8. 2003
    ..The dimerization of dPC can effectively account for the histone-binding specificity and provides new mechanistic insights into the function of Polycomb. We propose that self-association is functionally important for Polycomb...
  85. ncbi request reprint [Screening, cloning, and analyzing for hSNF5 binding proteins in human fetal brain]
    Yi Zhang
    National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China
    Zhongguo Yi Xue Ke Xue Yuan Xue Bao 26:529-32. 2004
    ..To identify novel binding proteins of hSNF5, a subunit of chromatin remodeling complex in human fetal brain...
  86. ncbi request reprint Dimethyl sulfoxide targets phage RNA polymerases to promote transcription
    Zhiqiang Chen
    State Key Laboratory of Virology and Department of Biotechnology, College of Life Sciences, Wuhan University, Wuhan, Hubei 430072, China
    Biochem Biophys Res Commun 333:664-70. 2005
    ..DMSO-induced alteration of the polymerase structure and function highlights a potentially generalized mechanism in interpreting the molecular action of this popular solvent...
  87. ncbi request reprint Isolation and characterization of 18 genes encoding alpha- and beta-expansins in wheat (Triticum aestivum L.)
    Zhan Lin
    Department of Plant Genetics and Breeding, Key Laboratory of Crop Genomics and Genetic Improvement, Ministry of Agriculture Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing, 100094, China
    Mol Genet Genomics 274:548-56. 2005
    ..We therefore conclude that expansins are encoded by a multigene family in wheat, and could play important roles in growth and development...
  88. pmc Crystal structure of a peptidoglycan glycosyltransferase suggests a model for processive glycan chain synthesis
    Yanqiu Yuan
    Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 104:5348-53. 2007
    ..An analysis of the structure, combined with biochemical information showing that these enzymes are processive, suggests a model for glycan chain polymerization...
  89. ncbi request reprint Chronic restraint stress modulates expression of genes in murine spleen
    Deling Yin
    Department of Internal Medicine, James Quillen College of Medicine, East Tennessee State University, P O Box 70622, Johnson City, TN 37614, United States
    J Neuroimmunol 177:11-7. 2006
    ..In this study, we identify a potentially important component of pro-apoptotic activity in restraint stress and suggest a possible target for anti-apoptotic therapy to protect splenocytes against stress-induced apoptosis...
  90. ncbi request reprint Actions of neuropoietic cytokines and cyclic AMP in regenerative conditioning of rat primary sensory neurons
    Dongsheng Wu
    Center for Neuroscience, Institute of Cell and Molecular Sciences, Barts and The London, Queen Mary s School of Medicine, University of London, 4 Newark Street, London E1 2AT, UK
    Exp Neurol 204:66-76. 2007
    ....
  91. pmc MES-4: an autosome-associated histone methyltransferase that participates in silencing the X chromosomes in the C. elegans germ line
    Laurel B Bender
    Department of Biology, Indiana University, Bloomington, IN 47405, USA
    Development 133:3907-17. 2006
    ..We discuss how an autosomally associated HMT may participate in silencing genes on the X chromosome, in coordination with the direct silencing effects of the other MES proteins...
  92. ncbi request reprint The neuronal Ca2+ sensor protein visinin-like protein-1 is expressed in pancreatic islets and regulates insulin secretion
    Feihan F Dai
    Department of Physiology, University of Toronto, Toronto, Ontario M5S 1A8, Canada
    J Biol Chem 281:21942-53. 2006
    ..Increased VILIP-1 enhanced insulin secretion in a cAMP-associated manner. Down-regulation of VILIP-1 was accompanied by decreased cAMP accumulation but increased insulin gene transcription...
  93. ncbi request reprint Nerve growth factor promotes TLR4 signaling-induced maturation of human dendritic cells in vitro through inducible p75NTR 1
    Yingming Jiang
    Institute of Immunology and National Key Laboratory of Medical Immunology, Second Military Medical University, Shanghai, People s Republic of China
    J Immunol 179:6297-304. 2007
    ....
  94. ncbi request reprint Expression of endothelins and their receptors in nonmelanoma skin cancers
    Yi Zhang
    Department of Dermatology and Skin Science, University of British Columbia, Vancouver, Canada
    J Cutan Med Surg 10:269-76. 2006
    ..However, detailed investigation on endothelins in skin malignancies is lacking...
  95. ncbi request reprint SOCS3 suppresses AP-1 transcriptional activity in neuroblastoma cells through inhibition of c-Jun N-terminal kinase
    Tizong Miao
    Centre for Neuroscience, Queen Mary s School of Medicine, University of London, E1 2AT, London, UK
    Mol Cell Neurosci 37:367-75. 2008
    ..We conclude that endogenous SOCS3 inhibits AP-1 activity through blocking of JNK phosphorylation...
  96. pmc A modular network model of aging
    Huiling Xue
    Chinese Academy of Sciences Key Laboratory of Molecular and Developmental Biology, Center for Molecular Systems Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China
    Mol Syst Biol 3:147. 2007
    ..Network simulations further suggest that aging might preferentially attack key regulatory nodes that are important for the network stability, implicating a potential molecular basis for the stochastic nature of aging...
  97. pmc Quantitative proteomics of the archaeon Methanococcus maripaludis validated by microarray analysis and real time PCR
    Qiangwei Xia
    Department of Chemical Engineering, University of Washington, Seattle, Washington 98195, USA
    Mol Cell Proteomics 5:868-81. 2006
    ..15 ORFs were shown to be overexpressed by both methods, and two ORFs were shown to be overexpressed by proteomics and underexpressed by microarray...
  98. pmc A Bayesian Change point model for differential gene expression patterns of the DosR regulon of Mycobacterium tuberculosis
    Yi Zhang
    School of Crystallography, Birkbeck College, University of London, Malet Street, London, WC1E 7HX, UK
    BMC Genomics 9:87. 2008
    ....
  99. doi request reprint CD4-CD8 lineage commitment is regulated by a silencer element at the ThPOK transcription-factor locus
    Xi He
    Fox Chase Cancer Center, 7701 Burholme Ave, Philadelphia, PA 19111, USA
    Immunity 28:346-58. 2008
    ..We propose a silencer-dependent model of lineage choice, whereby inactivation of the DRE silencer by a strong TCR signal leads to CD4 commitment, whereas continued silencer activity leads to CD8 commitment...
  100. ncbi request reprint G9a-mediated irreversible epigenetic inactivation of Oct-3/4 during early embryogenesis
    Nirit Feldman
    Department of Experimental Medicine, Hebrew University Medical School, Ein Kerem, Jerusalem 91120, Israel
    Nat Cell Biol 8:188-94. 2006
    ..Genetic studies show that these epigenetic changes actually have an important role in the inhibition of Oct-3/4 re-expression, thereby preventing reprogramming...
  101. doi request reprint CITED2 signals through peroxisome proliferator-activated receptor-gamma to regulate death of cortical neurons after DNA damage
    Yasmilde Rodriguez Gonzalez
    Ottawa Health Research Institute, Neuroscience Group, Ottawa, Ontario, Canada
    J Neurosci 28:5559-69. 2008
    ..These results define a novel neuronal prodeath pathway in which Cdk4-mediated regulation of Cited2 activates PPARgamma and consequently caspase...

Research Grants17

  1. Role of histone methyltransferases hDOTIL in leukemogenesis
    Yi Zhang; Fiscal Year: 2009
    ..We will map the nuclear localization signal of hDOTIL and the AF10 interaction domains. We will also study how mis-targeting of hDOTIL by CALM-AF10 results in Hox gene upregulation and leukemogenesis. ..
  2. Mechanisms of Polycomb Silencing
    Yi Zhang; Fiscal Year: 2007
    ..5. .Determine the transcriptional effect and understand the mechanism of H3K27 methylation and H2A ubiquitylation using a reconstituted chromatin transcription system. More description in proposal. ..
  3. Role of histone methyltransferases hDOTIL in leukemogenesis
    Yi Zhang; Fiscal Year: 2007
    ..We will map the nuclear localization signal of hDOTIL and the AF10 interaction domains. We will also study how mis-targeting of hDOTIL by CALM-AF10 results in Hox gene upregulation and leukemogenesis. ..
  4. Histone Methylation in Polycomb Group Gene Silencing
    Yi Zhang; Fiscal Year: 2006
    ..Understanding how the EED-EZH2 complex mediates H3-K27 methylation in PcG silencing will add to our 9eneral understandincj of how epigenetic silencing is achieved. ..
  5. Mechanism of Gene Silencing by DNA Methylation
    Yi Zhang; Fiscal Year: 2005
    ..The proposed studies will provide a thorough characterization of the MeCP1 complex. They will also reveal the potential targets and repression mechanism of the MeCP1 complex. ..
  6. Mechanisms of Polycomb Silencing
    Yi Zhang; Fiscal Year: 2010
    ..5. .Determine the transcriptional effect and understand the mechanism of H3K27 methylation and H2A ubiquitylation using a reconstituted chromatin transcription system. More description in proposal. ..