Wenhui Zhang

Summary

Affiliation: University of Nebraska
Country: USA

Publications

  1. ncbi Maximization of production of secreted recombinant proteins in Pichia pastoris fed-batch fermentation
    Wenhui Zhang
    Biological Process Development Facility, Department of Chemical Engineering, University of Nebraska-Lincoln, Nebraska 68588-0643, USA
    Biotechnol Prog 21:386-93. 2005
  2. ncbi Design of methanol Feed control in Pichia pastoris fermentations based upon a growth model
    Wenhui Zhang
    Department of Chemical Engineering, University of Nebraska Lincoln, Lincoln, Nebraska 68583 0919, USA
    Biotechnol Prog 18:1392-9. 2002
  3. ncbi Pichia pastoris fermentation with mixed-feeds of glycerol and methanol: growth kinetics and production improvement
    Wenhui Zhang
    Department of Chemical Engineering, University of Nebraska Lincoln, 207M Othmer Hall, Lincoln, NE 68583 0919, USA
    J Ind Microbiol Biotechnol 30:210-5. 2003
  4. ncbi Optimization of cell density and dilution rate in Pichia pastoris continuous fermentations for production of recombinant proteins
    Wenhui Zhang
    Biological Process Development Facility, Department of Chemical Engineering, University of Nebraska-Lincoln, 207 Othmer Hall, Lincoln, NE 68588-0643, USA
    J Ind Microbiol Biotechnol 31:330-4. 2004
  5. ncbi Glycerophosphate as a phosphorus source in a defined medium for Pichia pastoris fermentation
    Wenhui Zhang
    Biological Process Development Facility, Department of Chemical Engineering, University of Nebraska-Lincoln, NE, 68588, USA
    Appl Microbiol Biotechnol 72:139-44. 2006
  6. ncbi Scale-up of the fermentation and purification of the recombinant heavy chain fragment C of botulinum neurotoxin serotype F, expressed in Pichia pastoris
    Scott K Johnson
    Biological Process and Development Facility, University of Nebraska-Lincoln, Lincoln, NE 68583-0919, USA
    Protein Expr Purif 32:1-9. 2003
  7. ncbi Improved production of recombinant ovine interferon-tau by mut(+) strain of Pichia pastoris using an optimized methanol feed profile
    Jayanta Sinha
    Biological Process Development Facility, Department of Chemical Engineering, University of Nebraska-Lincoln, Lincoln, Nebraska 68583, USA
    Biotechnol Prog 19:794-802. 2003
  8. ncbi Saturation of the secretory pathway by overexpression of a hookworm (Necator americanus) Protein (Na-ASP1)
    Mehmet Inan
    Department of Chemical Engineering, University of Nebraska, Lincoln, NE USA
    Methods Mol Biol 389:65-76. 2007
  9. ncbi Rational design and optimization of fed-batch and continuous fermentations
    Wenhui Zhang
    Xoma Ltd, Berkey, CA, USA
    Methods Mol Biol 389:43-64. 2007

Collaborators

Detail Information

Publications9

  1. ncbi Maximization of production of secreted recombinant proteins in Pichia pastoris fed-batch fermentation
    Wenhui Zhang
    Biological Process Development Facility, Department of Chemical Engineering, University of Nebraska-Lincoln, Nebraska 68588-0643, USA
    Biotechnol Prog 21:386-93. 2005
    ..The approaches proposed here greatly simplify the computational processes and validate the optimization strategy as a generalized approach to maximize the yield from fed-batch fermentations...
  2. ncbi Design of methanol Feed control in Pichia pastoris fermentations based upon a growth model
    Wenhui Zhang
    Department of Chemical Engineering, University of Nebraska Lincoln, Lincoln, Nebraska 68583 0919, USA
    Biotechnol Prog 18:1392-9. 2002
    ..The method developed in this paper is robust and can serve as a framework for the design of other PID feedback control systems in biological processes...
  3. ncbi Pichia pastoris fermentation with mixed-feeds of glycerol and methanol: growth kinetics and production improvement
    Wenhui Zhang
    Department of Chemical Engineering, University of Nebraska Lincoln, 207M Othmer Hall, Lincoln, NE 68583 0919, USA
    J Ind Microbiol Biotechnol 30:210-5. 2003
    ..The results show that the optimal desired micro (gly)/ micro (MeOH) is around 2 for obtaining the highest BoNT/C(Hc) protein content in cells: about 3 mg/g wet cells...
  4. ncbi Optimization of cell density and dilution rate in Pichia pastoris continuous fermentations for production of recombinant proteins
    Wenhui Zhang
    Biological Process Development Facility, Department of Chemical Engineering, University of Nebraska-Lincoln, 207 Othmer Hall, Lincoln, NE 68588-0643, USA
    J Ind Microbiol Biotechnol 31:330-4. 2004
    ..The approach developed in this study is expected to be applicable to the optimization of continuous fermentations by other microorganisms...
  5. ncbi Glycerophosphate as a phosphorus source in a defined medium for Pichia pastoris fermentation
    Wenhui Zhang
    Biological Process Development Facility, Department of Chemical Engineering, University of Nebraska-Lincoln, NE, 68588, USA
    Appl Microbiol Biotechnol 72:139-44. 2006
    ..The results from this work demonstrate that sodium glycerophosphate can be assimilated by the P. pastoris strains and can be employed as a reliable phosphorus source for both cell growth and recombinant protein production...
  6. ncbi Scale-up of the fermentation and purification of the recombinant heavy chain fragment C of botulinum neurotoxin serotype F, expressed in Pichia pastoris
    Scott K Johnson
    Biological Process and Development Facility, University of Nebraska-Lincoln, Lincoln, NE 68583-0919, USA
    Protein Expr Purif 32:1-9. 2003
    ..The ratios of the two clipped forms were consistent from the bench to pilot scales. Purified BoNTF(Hc) at the pilot scale was found to sufficiently protect mice against a high dose of BoNTF neurotoxin...
  7. ncbi Improved production of recombinant ovine interferon-tau by mut(+) strain of Pichia pastoris using an optimized methanol feed profile
    Jayanta Sinha
    Biological Process Development Facility, Department of Chemical Engineering, University of Nebraska-Lincoln, Lincoln, Nebraska 68583, USA
    Biotechnol Prog 19:794-802. 2003
    ....
  8. ncbi Saturation of the secretory pathway by overexpression of a hookworm (Necator americanus) Protein (Na-ASP1)
    Mehmet Inan
    Department of Chemical Engineering, University of Nebraska, Lincoln, NE USA
    Methods Mol Biol 389:65-76. 2007
    ..However, increasing gene copy number of Na-ASP1 protein in P. pastoris saturated secretory capacity and therefore, decreased the amount of secreted protein in clones harboring multiple copies of Na-ASP1 gene...
  9. ncbi Rational design and optimization of fed-batch and continuous fermentations
    Wenhui Zhang
    Xoma Ltd, Berkey, CA, USA
    Methods Mol Biol 389:43-64. 2007
    ..This protocol can be used as a practical manual for process development of a P. pastoris recombinant fermentation, and also as a reference for fermentation of other microorganisms...