Affiliation: University of North Carolina
- Respiratory syncytial virus infection of human airway epithelial cells is polarized, specific to ciliated cells, and without obvious cytopathologyLiqun Zhang
Cystic Fibrosis Pulmonary Research and Treatment Center, University of North Carolina, Chapel Hill, North Carolina 27599 7248, USA
J Virol 76:5654-66. 2002..Since rgRSV appears to breach the lumenal barriers encountered by other gene transfer vectors in the airway, this virus may be a good candidate for the development of a gene transfer vector for CF lung disease...
- Comparison of differing cytopathic effects in human airway epithelium of parainfluenza virus 5 (W3A), parainfluenza virus type 3, and respiratory syncytial virusLiqun Zhang
Cystic Fibrosis Pulmonary Research and Treatment Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
Virology 421:67-77. 2011..These studies revealed striking differences in cytopathology of PIV5 versus PIV3 or RSV and indicate the extent of cytopathology determined in cell-lines does not predict events in differentiated airway cells...
- α-Fetoprotein gene delivery to the nasal epithelium of nonhuman primates by human parainfluenza viral vectorsLiqun Zhang
Cystic Fibrosis Pulmonary Research and Treatment Center, University of North Carolina at Chapel Hill, 27759, USA
Hum Gene Ther 21:1657-64. 2010..In summary, rhAFP proved suitable for monitoring in vivo gene delivery over time, and PIV vectors appear to be promising airway-specific gene transfer vehicles that warrant further development...
- Coupled nucleotide and mucin hypersecretion from goblet-cell metaplastic human airway epitheliumSeiko F Okada
Cystic Fibrosis Pulmonary Research and Treatment Center, The University of North Carolina at Chapel Hill, 27599, USA
Am J Respir Cell Mol Biol 45:253-60. 2011....
- CFTR delivery to 25% of surface epithelial cells restores normal rates of mucus transport to human cystic fibrosis airway epitheliumLiqun Zhang
CF Pulmonary Research and Treatment Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA
PLoS Biol 7:e1000155. 2009....
- Generation of novel AAV variants by directed evolution for improved CFTR delivery to human ciliated airway epitheliumWuping Li
Gene Therapy Center, University of North Carolina, Chapel Hill, North Carolina 27599 7352, USA
Mol Ther 17:2067-77. 2009..These results suggest that directed evolution of AAV on relevant in vitro models will enable further improvements in CFTR gene transfer efficiency and the development of an efficacious and safe gene transfer vector for CF lung disease...
- AAV exploits subcellular stress associated with inflammation, endoplasmic reticulum expansion, and misfolded proteins in models of cystic fibrosisJarrod S Johnson
Department of Pharmacology, University of North Carolina, Chapel Hill, North Carolina, United States of America
PLoS Pathog 7:e1002053. 2011....
- The effect of respiratory synctial virus on chemokine release by differentiated airway epitheliumThomas E Mellow
Department of Pediatrics, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599-7220, USA
Exp Lung Res 30:43-57. 2004..In epithelial layers, virus-containing cells are the predominant source of the increased chemokine release. The authors speculate that similar processes in vivo influence recruitment of leukocytes to sites of RSV infection...
- Infection of ciliated cells by human parainfluenza virus type 3 in an in vitro model of human airway epitheliumLiqun Zhang
CF/Pulmonary Research and Treatment Center, 7021 Thurston Bowles, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7248, USA
J Virol 79:1113-24. 2005..HAE derived from cystic fibrosis patients was not more susceptible to rgPIV3 infection but did exhibit limited spread of virus due to impaired movement of lumenal secretions due to compromised function of the cilia...
- Normal and cystic fibrosis airway surface liquid homeostasis. The effects of phasic shear stress and viral infectionsRobert Tarran
Cystic Fibrosis/Pulmonary Research and Treatment Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599-7248, USA
J Biol Chem 280:35751-9. 2005....
- Mesd encodes an LRP5/6 chaperone essential for specification of mouse embryonic polarityJen Chih Hsieh
Department of Biochemistry and Cell Biology, Center for Developmental Genetics, State University of New York, Stony Brook, Stony Brook, NY 11794, USA
Cell 112:355-67. 2003....
- Optimization of adenoviral vectors to direct highly amplified prostate-specific expression for imaging and gene therapyMakoto Sato
Department of Urology, University of California at Los Angeles School of Medicine, Los Angeles, California 90095, USA
Mol Ther 8:726-37. 2003..Based on these findings, we hope to refine the TSTA Ads further to improve the efficacy and safety of prostate cancer gene therapy...
- Interrogating androgen receptor function in recurrent prostate cancerLiqun Zhang
Departments of Biological Chemistry, University of California Los Angeles School of Medicine, Los Angeles, CA 90095, USA
Cancer Res 63:4552-60. 2003..Our data support the concept that AR is fully functional in recurrent cancer and suggest a model by which a poised but largely inactive transcription complex facilitates reactivation by AR at castrate levels of ligand...
- Noninvasive imaging of enhanced prostate-specific gene expression using a two-step transcriptional amplification-based lentivirus vectorMeera Iyer
The Crump Institute for Molecular Imaging, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, CA 90095, USA
Mol Ther 10:545-52. 2004....
- Functionality of androgen receptor-based gene expression imaging in hormone refractory prostate cancerMakoto Sato
Department of Urology, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, California, USA
Clin Cancer Res 11:3743-9. 2005..Together with the fact that majority of recurrent prostate cancers express AR and PSA, we foresee that the TSTA approach can be a promising gene therapy strategy for the advanced stages of prostate cancer...