Jin Zhang

Summary

Affiliation: University of Maryland
Country: USA

Publications

  1. pmc Low-dose ouabain constricts small arteries from ouabain-hypertensive rats: implications for sustained elevation of vascular resistance
    Jin Zhang
    Department of Physiology, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    Am J Physiol Heart Circ Physiol 297:H1140-50. 2009
  2. pmc Knockout of Na+/Ca2+ exchanger in smooth muscle attenuates vasoconstriction and L-type Ca2+ channel current and lowers blood pressure
    Jin Zhang
    Department of Physiology, University of Maryland School of Medicine, 655 W Baltimore St, Baltimore, MD 21201, USA
    Am J Physiol Heart Circ Physiol 298:H1472-83. 2010
  3. doi request reprint Intravital Förster resonance energy transfer imaging reveals elevated [Ca2+]i and enhanced sympathetic tone in femoral arteries of angiotensin II-infused hypertensive biosensor mice
    Youhua Wang
    J Zhang Department of Physiology, University of Maryland School of Medicine, 655 W Baltimore Street, Baltimore, MD 21201, USA
    J Physiol 591:5321-36. 2013
  4. doi request reprint New insights into the contribution of arterial NCX to the regulation of myogenic tone and blood pressure
    Jin Zhang
    Department of Physiology, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    Adv Exp Med Biol 961:329-43. 2013
  5. pmc In vivo assessment of artery smooth muscle [Ca2+]i and MLCK activation in FRET-based biosensor mice
    Jin Zhang
    Dept of Physiology, Univ of Maryland School of Medicine, 655 W Baltimore St, Baltimore, MD 21201, USA
    Am J Physiol Heart Circ Physiol 299:H946-56. 2010
  6. pmc Activation of L-type Ca2+ channels by protein kinase C is reduced in smooth muscle-specific Na+/Ca2+ exchanger knockout mice
    Chongyu Ren
    Department of Physiology, University of Maryland School of Medicine, 655 W Baltimore St, Baltimore, MD 21201, USA
    Am J Physiol Heart Circ Physiol 298:H1484-91. 2010
  7. doi request reprint Dynamic visualization of signaling activities in living cells
    Michael D Allen
    Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Sci Signal 1:pt6. 2008
  8. pmc Visualization of JNK activity dynamics with a genetically encoded fluorescent biosensor
    Matthew Fosbrink
    Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 107:5459-64. 2010
  9. doi request reprint The structure and function of fluorescent proteins
    Vedangi Sample
    Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Chem Soc Rev 38:2852-64. 2009
  10. pmc The Na+/Ca2+ exchanger-1 mediates left ventricular dysfunction in mice with chronic intermittent hypoxia
    Ling Chen
    Department of Medicine, University of Maryland, Baltimore, Maryland, USA
    J Appl Physiol (1985) 109:1675-85. 2010

Collaborators

Detail Information

Publications66

  1. pmc Low-dose ouabain constricts small arteries from ouabain-hypertensive rats: implications for sustained elevation of vascular resistance
    Jin Zhang
    Department of Physiology, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    Am J Physiol Heart Circ Physiol 297:H1140-50. 2009
    ..66 nM should raise resistance by approximately 35%. We conclude that dynamic constriction in response to circulating nanomolar ouabain in small arteries likely makes a major contribution to the increased vascular tone and BP in OH rats...
  2. pmc Knockout of Na+/Ca2+ exchanger in smooth muscle attenuates vasoconstriction and L-type Ca2+ channel current and lowers blood pressure
    Jin Zhang
    Department of Physiology, University of Maryland School of Medicine, 655 W Baltimore St, Baltimore, MD 21201, USA
    Am J Physiol Heart Circ Physiol 298:H1472-83. 2010
    ..In NCX1(SM-/-) mouse artery myocytes, the reduced Ca(2+) entry via NCX1 may lower cytosolic Ca(2+) concentration and thereby reduce MT and BP. The reduced LVGC activity may be the consequence of a low cytosolic Ca(2+) concentration...
  3. doi request reprint Intravital Förster resonance energy transfer imaging reveals elevated [Ca2+]i and enhanced sympathetic tone in femoral arteries of angiotensin II-infused hypertensive biosensor mice
    Youhua Wang
    J Zhang Department of Physiology, University of Maryland School of Medicine, 655 W Baltimore Street, Baltimore, MD 21201, USA
    J Physiol 591:5321-36. 2013
    ..Evidence of altered artery reactivity or remodeling was not found...
  4. doi request reprint New insights into the contribution of arterial NCX to the regulation of myogenic tone and blood pressure
    Jin Zhang
    Department of Physiology, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    Adv Exp Med Biol 961:329-43. 2013
    ..Arterial NCX expression and mechanisms that control the local (sub-plasma membrane) Na(+) gradient, including cation-selective receptor-operated channels containing TRPC6, regulate arterial Ca(2+) and constriction, and thus BP...
  5. pmc In vivo assessment of artery smooth muscle [Ca2+]i and MLCK activation in FRET-based biosensor mice
    Jin Zhang
    Dept of Physiology, Univ of Maryland School of Medicine, 655 W Baltimore St, Baltimore, MD 21201, USA
    Am J Physiol Heart Circ Physiol 299:H946-56. 2010
    ..Based on the observed variance of the FRET data, this method should enable the detection of a difference in basal [Ca(2+)](i) of 29 nM between two groups of 12 mice with a significance of P < 0.05...
  6. pmc Activation of L-type Ca2+ channels by protein kinase C is reduced in smooth muscle-specific Na+/Ca2+ exchanger knockout mice
    Chongyu Ren
    Department of Physiology, University of Maryland School of Medicine, 655 W Baltimore St, Baltimore, MD 21201, USA
    Am J Physiol Heart Circ Physiol 298:H1484-91. 2010
    ..We conclude that in NCX1(SM-/-) myocytes, reduced Ca(2+) entry via NCX1 lowers cytosolic [Ca(2+)], thereby reducing PKC activation that lowers LVGC activation...
  7. doi request reprint Dynamic visualization of signaling activities in living cells
    Michael D Allen
    Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Sci Signal 1:pt6. 2008
    ..Together, these reporters have the potential to provide important spatiotemporal information about the circuitry governing specific signaling events in living cells...
  8. pmc Visualization of JNK activity dynamics with a genetically encoded fluorescent biosensor
    Matthew Fosbrink
    Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 107:5459-64. 2010
    ..The development of JNKAR1, therefore, laid a foundation for evaluating the signaling properties and behaviors of the JNK cascade in single living cells...
  9. doi request reprint The structure and function of fluorescent proteins
    Vedangi Sample
    Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Chem Soc Rev 38:2852-64. 2009
    ....
  10. pmc The Na+/Ca2+ exchanger-1 mediates left ventricular dysfunction in mice with chronic intermittent hypoxia
    Ling Chen
    Department of Medicine, University of Maryland, Baltimore, Maryland, USA
    J Appl Physiol (1985) 109:1675-85. 2010
    ..We conclude that myocardial NCX1 does not mediate changes in blood pressure, but is one of the mediators for LV global dysfunction and cardiomyocyte injury in CIH...
  11. doi request reprint Parallel tracking of cAMP and PKA signaling dynamics in living cells with FRET-based fluorescent biosensors
    Nwe Nwe Aye-Han
    Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Mol Biosyst 8:1435-40. 2012
    ....
  12. pmc Sodium pump alpha2 subunits control myogenic tone and blood pressure in mice
    Jin Zhang
    Department of Physiology, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    J Physiol 569:243-56. 2005
    ..Accordingly, in salt-dependent hypertension, EOLC probably increases vascular resistance and blood pressure by reducing alpha2 Na(+) pump activity and promoting Ca(2)(+) entry via NCX in myocytes...
  13. ncbi request reprint Mg2+ blocks myogenic tone but not K+-induced constriction: role for SOCs in small arteries
    Jin Zhang
    Department of Physiology, University of Maryland School of Medicine, Baltimore 21201, USA
    Am J Physiol Heart Circ Physiol 283:H2692-705. 2002
    ..The data suggest that Ca(2+) entry through SOCs helps maintain both myogenic tone and alpha(1)-adrenoceptor-induced tonic vasoconstriction...
  14. ncbi request reprint On the mechanism of myogenic tone in small arteries
    Mordecai P Blaustein
    Department of Physiology, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    J Muscle Res Cell Motil 25:615. 2004
  15. ncbi request reprint Role of Cav1.2 L-type Ca2+ channels in vascular tone: effects of nifedipine and Mg2+
    Jin Zhang
    Dept of Physiology, Univ of Maryland School of Medicine, 655 W Baltimore St, Baltimore, MD 21201, USA
    Am J Physiol Heart Circ Physiol 292:H415-25. 2007
    ..The results indicate that Mg(2+) relaxes MT by inhibiting Ca(2+) influx through LVGCs. These data provide new information about the central role of Ca(v)1.2 LVGCs in generating and maintaining MT in mouse mesenteric small arteries...
  16. pmc Direct activation of Epac by sulfonylurea is isoform selective
    Katie J Herbst
    Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Chem Biol 18:243-51. 2011
    ..We also show that SUs selectively activate Epac2 isoform, but not the closely related Epac1, further establishing SUs as a new class of isoform-selective enzyme activators...
  17. pmc Signaling diversity of PKA achieved via a Ca2+-cAMP-PKA oscillatory circuit
    Qiang Ni
    Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Nat Chem Biol 7:34-40. 2011
    ....
  18. pmc Global analysis of phosphorylation networks in humans
    Jianfei Hu
    Department of Ophthalmology, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA
    Biochim Biophys Acta 1844:224-31. 2014
    ..This article is part of a Special Issue entitled: Computational Proteomics, Systems Biology & Clinical Implications. Guest Editor: Yudong Cai. ..
  19. pmc Multiplexed visualization of dynamic signaling networks using genetically encoded fluorescent protein-based biosensors
    CHARLENE DEPRY
    Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Pflugers Arch 465:373-81. 2013
    ....
  20. pmc Regulation of nuclear PKA revealed by spatiotemporal manipulation of cyclic AMP
    Vedangi Sample
    Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Nat Chem Biol 8:375-82. 2012
    ....
  21. doi request reprint Chapter 2: Molecular sensors based on fluorescence resonance energy transfer to visualize cellular dynamics
    Bharath Ananthanarayanan
    Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Methods Cell Biol 89:37-57. 2008
    ..Furthermore, a case study of the PI3K/Akt signaling pathway using a series of FRET sensors highlights the tremendous potential of the method in exploring relevant biological systems...
  22. pmc A phosphorylation-dependent intramolecular interaction regulates the membrane association and activity of the tumor suppressor PTEN
    Meghdad Rahdar
    Departments of Cell Biology and Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 106:480-5. 2009
    ..Small molecules targeting these interactions could potentially serve as therapeutic agents in antagonizing Ras or PI3K-driven tumors. The study also stresses the importance of determining the structure of the native enzyme...
  23. ncbi request reprint Subcellular dynamics of protein kinase A activity visualized by FRET-based reporters
    Michael D Allen
    Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Biochem Biophys Res Commun 348:716-21. 2006
    ..Targeting AKAR3 to outer mitochondrial membrane revealed that basal PKA activity at mitochondria differs from that in the cytoplasm, indicating differential regulation of PKA activity at different subcellular locations...
  24. pmc Analysis of serotonin N-acetyltransferase regulation in vitro and in live cells using protein semisynthesis
    Lawrence M Szewczuk
    Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Biochemistry 47:10407-19. 2008
    ..These studies offer new insights into the molecular basis of melatonin regulation and 14-3-3zeta interaction...
  25. doi request reprint Bicarbonate-sensitive soluble and transmembrane adenylyl cyclases in peripheral chemoreceptors
    Ana R Nunes
    Department of Pediatrics, Johns Hopkins Medical Institutions, Baltimore, MD 21287 3200, USA
    Respir Physiol Neurobiol 188:83-93. 2013
    ..sAC and tmAC are functional in CB but intracellular elevations in CO2/HCO3(-) in IH conditions on their own are insufficient to further activate these enzymes, suggesting that the hypercapnic response is dependent on secondary acidosis. ..
  26. doi request reprint Visualizing dynamic activities of signaling enzymes using genetically encodable FRET-based biosensors from designs to applications
    Xin Zhou
    Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Methods Enzymol 504:317-40. 2012
    ..Finally, an example of using both uni- and bimolecular kinase activity reporters to visualize PKA activity in living cells will be presented to provide practical tips for using these biosensors to explore specific biological systems...
  27. pmc How NaCl raises blood pressure: a new paradigm for the pathogenesis of salt-dependent hypertension
    Mordecai P Blaustein
    Dept of Physiology, Univ of Maryland School of Medicine, 655 W Baltimore St, Baltimore, MD, 21201, USA
    Am J Physiol Heart Circ Physiol 302:H1031-49. 2012
    ..These several central and peripheral mechanisms are coordinated, in part by EO, to effect and maintain the salt-induced elevation of BP...
  28. pmc PI3K/Akt signaling requires spatial compartmentalization in plasma membrane microdomains
    Xinxin Gao
    Department of Pharmacology, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 108:14509-14. 2011
    ..They further suggest that dysregulation of this compartmentalization may underlie pathological complications such as insulin resistance...
  29. doi request reprint Visualization of phosphatase activity in living cells with a FRET-based calcineurin activity sensor
    Robert H Newman
    Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Mol Biosyst 4:496-501. 2008
    ..The successful design of a prototype phosphatase activity sensor lays a foundation for studying targeting and compartmentation of phosphatases...
  30. ncbi request reprint Live-cell molecular analysis of Akt activation reveals roles for activation loop phosphorylation
    Bharath Ananthanarayanan
    Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    J Biol Chem 282:36634-41. 2007
    ..These studies uncover new regulatory roles of this critical phosphorylation event of Akt for ensuring its proper activation and function...
  31. pmc PhosphoNetworks: a database for human phosphorylation networks
    Jianfei Hu
    Department of Ophthalmology, Johns Hopkins School of Medicine, Department of Pharmacology and Molecular Sciences, Center for High Throughput Biology, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA, Department of Biology, North Carolina Agricultural and Technical State University, Greensboro, NC 27411, USA and The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA
    Bioinformatics 30:141-2. 2014
    ..In addition, the database also includes several analytical tools for dissecting phosphorylation networks. PhosphoNetworks is expected to play a prominent role in proteomics and phosphorylation-related disease research...
  32. doi request reprint Vascular tone and Ca(2+) signaling in murine cremaster muscle arterioles in vivo
    Joseph R H Mauban
    Department of Physiology, School of Medicine, University of Maryland Baltimore, Baltimore, Maryland 21201, USA
    Microcirculation 20:269-77. 2013
    ..We sought to determine some of the molecular requirements for basal state "tone" of skeletal muscle arterioles in vivo, and whether asynchronous Ca(2+) waves are involved or not...
  33. pmc Live-cell studies of p300/CBP histone acetyltransferase activity and inhibition
    Beverley M Dancy
    Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205, USA
    Chembiochem 13:2113-21. 2012
    ..Additionally, we describe a new p300/CBP HAT inhibitor, C107, and show that it can also increase cellular Histac FRET. Taken together, these studies provide a live-cell strategy for identifying and evaluating p300/CBP inhibitors...
  34. pmc Identification of targets of c-Src tyrosine kinase by chemical complementation and phosphoproteomics
    Isabel Martinez Ferrando
    Department of Pharmacology and Molecular Sciences, Johns Hopkins School of Medicine, Baltimore, Maryland 21205, USA
    Mol Cell Proteomics 11:355-69. 2012
    ....
  35. doi request reprint Reporting from the field: genetically encoded fluorescent reporters uncover signaling dynamics in living biological systems
    Sohum Mehta
    Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Annu Rev Biochem 80:375-401. 2011
    ....
  36. pmc LRP6 mediates cAMP generation by G protein-coupled receptors through regulating the membrane targeting of Gα(s)
    Mei Wan
    Department of Orthopaedic Surgery, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Sci Signal 4:ra15. 2011
    ..Thus, we suggest that the binding of Gα(s) to LRP6 is required to establish a functional GPCR-Gα(s)-AC signaling pathway for the production of cAMP, providing an additional regulatory component to the current GPCR-cAMP paradigm...
  37. pmc Spatiotemporally regulated protein kinase A activity is a critical regulator of growth factor-stimulated extracellular signal-regulated kinase signaling in PC12 cells
    Katie J Herbst
    Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Mol Cell Biol 31:4063-75. 2011
    ....
  38. doi request reprint Visualization of PKA activity in plasma membrane microdomains
    CHARLENE DEPRY
    Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Mol Biosyst 7:52-8. 2011
    ....
  39. doi request reprint Visualization of kinase activity with FRET-based activity biosensors
    CHARLENE DEPRY
    Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Curr Protoc Mol Biol . 2010
    ..This unit describes a general protocol for utilizing KARs to visualize kinase activity in living mammalian cells with fluorescence microscopy...
  40. ncbi request reprint Ca2+ signaling in mouse mesenteric small arteries: myogenic tone and adrenergic vasoconstriction
    Joseph Zacharia
    Dept of Physiology, Univ of Maryland, Baltimore, MD 21201, USA
    Am J Physiol Heart Circ Physiol 292:H1523-32. 2007
    ..alpha(1)-Adrenoceptor-induced vasoconstriction may be supported either by Ca(2+) waves or by steady elevation of cytoplasmic [Ca(2+)], depending on the amount of MT...
  41. pmc Signal propagation from membrane messengers to nuclear effectors revealed by reporters of phosphoinositide dynamics and Akt activity
    Bharath Ananthanarayanan
    Department of Pharmacology, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 102:15081-6. 2005
    ..Thus, signal propagation from the lipid messengers at plasma membrane to the effectors in the nucleus is precisely controlled by kinases as well as lipid and protein phosphatases...
  42. ncbi request reprint How does salt retention raise blood pressure?
    Mordecai P Blaustein
    Department of Physiology, University of Maryland School of Medicine, 655 W Baltimore St, Baltimore, Maryland 21201, USA
    Am J Physiol Regul Integr Comp Physiol 290:R514-23. 2006
    ..Thus, endogenous ouabain, and vascular alpha2 Na+ pumps and NCX1, are critical links between salt and hypertension. New pharmacological agents that act on these molecular links have potential in the clinical management of hypertension...
  43. pmc Construction of human activity-based phosphorylation networks
    Robert H Newman
    Department of Pharmacology and Molecular Sciences, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA
    Mol Syst Biol 9:655. 2013
    ..Overall, these studies provide global insights into kinase-mediated signaling pathways and promise to advance our understanding of cellular signaling processes in humans...
  44. pmc Widely accessible method for superresolution fluorescence imaging of living systems
    Peter Dedecker
    Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 109:10909-14. 2012
    ..Furthermore, both 3D and multicolor imaging are readily achievable. Because of its ease of use and high performance, we anticipate that pcSOFI will prove an attractive approach for superresolution imaging...
  45. ncbi request reprint FRET-based biosensors for protein kinases: illuminating the kinome
    Jin Zhang
    Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, 725 N Wolfe Street, Baltimore, MD 21205, USA
    Mol Biosyst 3:759-65. 2007
    ..Highlighted in this article is the design of genetically-encodable, FRET-based kinase biosensors with examples of their implementation to study kinase regulation in live biological contexts with high spatial and temporal resolution...
  46. ncbi request reprint Left ventricular dysfunction and associated cellular injury in rats exposed to chronic intermittent hypoxia
    Ling Chen
    Div of Pulmonary and Critical Care Medicine, Univ of Maryland, Baltimore, 685 West Baltimore St, MSTF 816, Baltimore, MD 21201, USA
    J Appl Physiol (1985) 104:218-23. 2008
    ..In conclusion, CIH-mediated LV global dysfunction is associated with myocyte hypertrophy and apoptosis at the cellular level...
  47. doi request reprint Dynamic visualization of signal transduction in living cells: from second messengers to kinases
    Katie J Herbst
    Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, MD, USA
    IUBMB Life 61:902-8. 2009
    ....
  48. ncbi request reprint A tunable FRET circuit for engineering fluorescent biosensors
    Michael D Allen
    Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, 725 N Wolfe St, Baltimore, MD 21205, USA
    Angew Chem Int Ed Engl 47:500-2. 2008
  49. pmc Effect of oxygen on phosphodiesterases (PDE) 3 and 4 isoforms and PKA activity in the superior cervical ganglia
    Ana Rita Nunes
    Department of Pediatrics, Johns Hopkins University, Baltimore, MD 21287 3200, USA
    Adv Exp Med Biol 758:287-94. 2012
    ..5 ± 0.8 and 14.7 ± 0.8, respectively).PDE3a, PDE4b and PDE4d isoforms mRNAs were highly expressed in the whole SCG with no modulation by hypoxia...
  50. ncbi request reprint Chemical rescue of a mutant enzyme in living cells
    Yingfeng Qiao
    Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Science 311:1293-7. 2006
    ..Rescue of R388A cellular Src provided insights into the mitogen-activated protein kinase pathway. This chemical rescue approach will likely have many applications in cell signaling...
  51. pmc How cells process information: quantification of spatiotemporal signaling dynamics
    Ambhighainath Ganesan
    Department of Biomedical Engineering, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Protein Sci 21:918-28. 2012
    ..Finally, the authors illustrate the role of spatial compartmentalization in regulating cellular responses with examples of second-messenger signaling in cardiac myocytes...
  52. ncbi request reprint Analyzing protein kinase dynamics in living cells with FRET reporters
    Qiang Ni
    Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Methods 40:279-86. 2006
    ....
  53. pmc Fluorescent indicators of cAMP and Epac activation reveal differential dynamics of cAMP signaling within discrete subcellular compartments
    Lisa M DiPilato
    Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 101:16513-8. 2004
    ..Thus, cAMP dynamics and the activation of its effectors are precisely controlled spatiotemporally in vivo...
  54. pmc Mechanistic distinction between activation and inhibition of (Na(+)+K(+))-ATPase-mediated Ca2+ influx in cardiomyocytes
    Kai Y Xu
    Department of Surgery, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    Biochem Biophys Res Commun 406:200-3. 2011
    ....
  55. pmc Fluorescent biosensors for real-time tracking of post-translational modification dynamics
    Nwe Nwe Aye-Han
    Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Curr Opin Chem Biol 13:392-7. 2009
    ..In addition, we also provide discussions about various engineering strategies for overcoming potential challenges associated with the development and application of such biosensors...
  56. doi request reprint Using FRET-based reporters to visualize subcellular dynamics of protein kinase A activity
    CHARLENE DEPRY
    Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Methods Mol Biol 756:285-94. 2011
    ..This method details how to monitor real-time PKA activity dynamics in mammalian cells using fluorescence resonance energy transfer (FRET)-based reporters...
  57. pmc Spatiotemporal analysis of differential Akt regulation in plasma membrane microdomains
    Xinxin Gao
    Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Mol Biol Cell 19:4366-73. 2008
    ....
  58. pmc The cAMP-dependent protein kinase inhibitor H-89 attenuates the bioluminescence signal produced by Renilla Luciferase
    Katie J Herbst
    Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    PLoS ONE 4:e5642. 2009
    ..These cell-based assays are often utilized to test the involvement of PKA-dependent processes by using H-89, a reversible competitive inhibitor of PKA...
  59. doi request reprint Genetically encoded molecular probes to visualize and perturb signaling dynamics in living biological systems
    Vedangi Sample
    Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, 725 N Wolfe St, Baltimore, MD 21205, USA
    J Cell Sci 127:1151-60. 2014
    ....
  60. pmc Galphas-biased beta2-adrenergic receptor signaling from restoring synchronous contraction in the failing heart
    Khalid Chakir
    Division of Cardiology, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Sci Transl Med 3:100ra88. 2011
    ..These results identify a key pathway that is triggered by restoring contractile synchrony and that may represent a new therapeutic approach for a broad population of HF patients...
  61. doi request reprint The design and application of genetically encodable biosensors based on fluorescent proteins
    Robert H Newman
    Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Methods Mol Biol 1071:1-16. 2014
    ..In this introductory chapter, we first discuss the properties of GFP family members before turning our attention to the design and application of genetically encodable fluorescent biosensors to live cell imaging. ..
  62. doi request reprint Using a genetically encoded FRET-based reporter to visualize calcineurin phosphatase activity in living cells
    Sohum Mehta
    Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Methods Mol Biol 1071:139-49. 2014
    ..The following method describes how to monitor real-time calcineurin activity in cultured mammalian cells using a fluorescence resonance energy transfer (FRET)-based activity reporter. ..
  63. doi request reprint A multiparameter live cell imaging approach to monitor cyclic AMP and protein kinase A dynamics in parallel
    Nwe Nwe Aye-Han
    Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Methods Mol Biol 1071:207-15. 2014
    ..In this review, we detail a multiparameter live cell imaging method to monitor 3',5'-cyclic adenosine monophosphate (cAMP) levels and protein kinase A (PKA) activities in parallel. ..
  64. pmc Localizer: fast, accurate, open-source, and modular software package for superresolution microscopy
    Peter Dedecker
    The Johns Hopkins University School of Medicine, Department of Pharmacology and Molecular Sciences, 725 N Wolfe Street, Baltimore, Mayland 21205, USA
    J Biomed Opt 17:126008. 2012
    ..By dramatically improving the analysis performance and ensuring the easy addition of current and future enhancements, Localizer strongly improves the usability of superresolution imaging in a variety of biomedical studies...
  65. doi request reprint Dynamic visualization of cellular signaling
    Qiang Ni
    Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, MD, 21205, USA
    Adv Biochem Eng Biotechnol 119:79-97. 2010
    ..Here we review recent advances in the development of such biosensors and some biological insights revealed by these biosensors in living cells, tissue, and organisms...
  66. ncbi request reprint Adenosine diphosphate (ADP)-induced thromboxane A(2) generation in human platelets requires coordinated signaling through integrin alpha(IIb)beta(3) and ADP receptors
    Jianguo Jin
    Department of Physiology, Temple University Medical School, Philadelphia, PA 19140, USA
    Blood 99:193-8. 2002
    ....