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Genomes and Genes | Jin ZhangSummaryAffiliation: University of Maryland Country: USA Publications
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Publications
Low-dose ouabain constricts small arteries from ouabain-hypertensive rats: implications for sustained elevation of vascular resistanceJin Zhang
Department of Physiology, University of Maryland School of Medicine, Baltimore, MD 21201, USA
Am J Physiol Heart Circ Physiol 297:H1140-50. 2009..66 nM should raise resistance by approximately 35%. We conclude that dynamic constriction in response to circulating nanomolar ouabain in small arteries likely makes a major contribution to the increased vascular tone and BP in OH rats...- Knockout of Na+/Ca2+ exchanger in smooth muscle attenuates vasoconstriction and L-type Ca2+ channel current and lowers blood pressureJin Zhang
Department of Physiology, University of Maryland School of Medicine, 655 W Baltimore St, Baltimore, MD 21201, USA
Am J Physiol Heart Circ Physiol 298:H1472-83. 2010..In NCX1(SM-/-) mouse artery myocytes, the reduced Ca(2+) entry via NCX1 may lower cytosolic Ca(2+) concentration and thereby reduce MT and BP. The reduced LVGC activity may be the consequence of a low cytosolic Ca(2+) concentration... 
New insights into the contribution of arterial NCX to the regulation of myogenic tone and blood pressureJin Zhang
Department of Physiology, University of Maryland School of Medicine, Baltimore, MD 21201, USA
Adv Exp Med Biol 961:329-43. 2013..Arterial NCX expression and mechanisms that control the local (sub-plasma membrane) Na(+) gradient, including cation-selective receptor-operated channels containing TRPC6, regulate arterial Ca(2+) and constriction, and thus BP...- In vivo assessment of artery smooth muscle [Ca2+]i and MLCK activation in FRET-based biosensor miceJin Zhang
Dept of Physiology, Univ of Maryland School of Medicine, 655 W Baltimore St, Baltimore, MD 21201, USA
Am J Physiol Heart Circ Physiol 299:H946-56. 2010..Based on the observed variance of the FRET data, this method should enable the detection of a difference in basal [Ca(2+)](i) of 29 nM between two groups of 12 mice with a significance of P < 0.05... - Activation of L-type Ca2+ channels by protein kinase C is reduced in smooth muscle-specific Na+/Ca2+ exchanger knockout miceChongyu Ren
Department of Physiology, University of Maryland School of Medicine, 655 W Baltimore St, Baltimore, MD 21201, USA
Am J Physiol Heart Circ Physiol 298:H1484-91. 2010..We conclude that in NCX1(SM-/-) myocytes, reduced Ca(2+) entry via NCX1 lowers cytosolic [Ca(2+)], thereby reducing PKC activation that lowers LVGC activation... - Dynamic visualization of signaling activities in living cellsMichael D Allen
Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
Sci Signal 1:pt6. 2008..Together, these reporters have the potential to provide important spatiotemporal information about the circuitry governing specific signaling events in living cells... - The Na+/Ca2+ exchanger-1 mediates left ventricular dysfunction in mice with chronic intermittent hypoxiaLing Chen
Department of Medicine, University of Maryland, Baltimore, Maryland, USA
J Appl Physiol 109:1675-85. 2010..We conclude that myocardial NCX1 does not mediate changes in blood pressure, but is one of the mediators for LV global dysfunction and cardiomyocyte injury in CIH... - Visualization of JNK activity dynamics with a genetically encoded fluorescent biosensorMatthew Fosbrink
Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
Proc Natl Acad Sci U S A 107:5459-64. 2010..The development of JNKAR1, therefore, laid a foundation for evaluating the signaling properties and behaviors of the JNK cascade in single living cells... - The structure and function of fluorescent proteinsVedangi Sample
Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
Chem Soc Rev 38:2852-64. 2009.... - Parallel tracking of cAMP and PKA signaling dynamics in living cells with FRET-based fluorescent biosensorsNwe Nwe Aye-Han
Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
Mol Biosyst 8:1435-40. 2012.... - Sodium pump alpha2 subunits control myogenic tone and blood pressure in miceJin Zhang
Department of Physiology, University of Maryland School of Medicine, Baltimore, MD 21201, USA
J Physiol 569:243-56. 2005..Accordingly, in salt-dependent hypertension, EOLC probably increases vascular resistance and blood pressure by reducing alpha2 Na(+) pump activity and promoting Ca(2)(+) entry via NCX in myocytes... 
Mg2+ blocks myogenic tone but not K+-induced constriction: role for SOCs in small arteriesJin Zhang
Department of Physiology, University of Maryland School of Medicine, Baltimore 21201, USA
Am J Physiol Heart Circ Physiol 283:H2692-705. 2002..The data suggest that Ca(2+) entry through SOCs helps maintain both myogenic tone and alpha(1)-adrenoceptor-induced tonic vasoconstriction...- On the mechanism of myogenic tone in small arteriesMordecai P Blaustein
Department of Physiology, University of Maryland School of Medicine, Baltimore, MD 21201, USA
J Muscle Res Cell Motil 25:615. 2004 - Role of Cav1.2 L-type Ca2+ channels in vascular tone: effects of nifedipine and Mg2+Jin Zhang
Dept of Physiology, Univ of Maryland School of Medicine, 655 W Baltimore St, Baltimore, MD 21201, USA
Am J Physiol Heart Circ Physiol 292:H415-25. 2007..The results indicate that Mg(2+) relaxes MT by inhibiting Ca(2+) influx through LVGCs. These data provide new information about the central role of Ca(v)1.2 LVGCs in generating and maintaining MT in mouse mesenteric small arteries... - Direct activation of Epac by sulfonylurea is isoform selectiveKatie J Herbst
Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
Chem Biol 18:243-51. 2011..We also show that SUs selectively activate Epac2 isoform, but not the closely related Epac1, further establishing SUs as a new class of isoform-selective enzyme activators... - Signaling diversity of PKA achieved via a Ca2+-cAMP-PKA oscillatory circuitQiang Ni
Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
Nat Chem Biol 7:34-40. 2011.... - Regulation of nuclear PKA revealed by spatiotemporal manipulation of cyclic AMPVedangi Sample
Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
Nat Chem Biol 8:375-82. 2012.... - Chapter 2: Molecular sensors based on fluorescence resonance energy transfer to visualize cellular dynamicsBharath Ananthanarayanan
Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
Methods Cell Biol 89:37-57. 2008..Furthermore, a case study of the PI3K/Akt signaling pathway using a series of FRET sensors highlights the tremendous potential of the method in exploring relevant biological systems... - Analysis of serotonin N-acetyltransferase regulation in vitro and in live cells using protein semisynthesisLawrence M Szewczuk
Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
Biochemistry 47:10407-19. 2008..These studies offer new insights into the molecular basis of melatonin regulation and 14-3-3zeta interaction... - A phosphorylation-dependent intramolecular interaction regulates the membrane association and activity of the tumor suppressor PTENMeghdad Rahdar
Departments of Cell Biology and Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
Proc Natl Acad Sci U S A 106:480-5. 2009..Small molecules targeting these interactions could potentially serve as therapeutic agents in antagonizing Ras or PI3K-driven tumors. The study also stresses the importance of determining the structure of the native enzyme... - Subcellular dynamics of protein kinase A activity visualized by FRET-based reportersMichael D Allen
Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
Biochem Biophys Res Commun 348:716-21. 2006..Targeting AKAR3 to outer mitochondrial membrane revealed that basal PKA activity at mitochondria differs from that in the cytoplasm, indicating differential regulation of PKA activity at different subcellular locations... - Visualizing dynamic activities of signaling enzymes using genetically encodable FRET-based biosensors from designs to applicationsXin Zhou
Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
Methods Enzymol 504:317-40. 2012..Finally, an example of using both uni- and bimolecular kinase activity reporters to visualize PKA activity in living cells will be presented to provide practical tips for using these biosensors to explore specific biological systems... - How NaCl raises blood pressure: a new paradigm for the pathogenesis of salt-dependent hypertensionMordecai P Blaustein
Dept of Physiology, Univ of Maryland School of Medicine, 655 W Baltimore St, Baltimore, MD, 21201, USA
Am J Physiol Heart Circ Physiol 302:H1031-49. 2012..These several central and peripheral mechanisms are coordinated, in part by EO, to effect and maintain the salt-induced elevation of BP... - PI3K/Akt signaling requires spatial compartmentalization in plasma membrane microdomainsXinxin Gao
Department of Pharmacology, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
Proc Natl Acad Sci U S A 108:14509-14. 2011..They further suggest that dysregulation of this compartmentalization may underlie pathological complications such as insulin resistance... - Live-cell molecular analysis of Akt activation reveals roles for activation loop phosphorylationBharath Ananthanarayanan
Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
J Biol Chem 282:36634-41. 2007..These studies uncover new regulatory roles of this critical phosphorylation event of Akt for ensuring its proper activation and function... - Visualization of phosphatase activity in living cells with a FRET-based calcineurin activity sensorRobert H Newman
Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, MD, USA
Mol Biosyst 4:496-501. 2008..The successful design of a prototype phosphatase activity sensor lays a foundation for studying targeting and compartmentation of phosphatases... - Live-cell studies of p300/CBP histone acetyltransferase activity and inhibitionBeverley M Dancy
Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205, USA
Chembiochem 13:2113-21. 2012..Additionally, we describe a new p300/CBP HAT inhibitor, C107, and show that it can also increase cellular Histac FRET. Taken together, these studies provide a live-cell strategy for identifying and evaluating p300/CBP inhibitors... - Identification of targets of c-Src tyrosine kinase by chemical complementation and phosphoproteomicsIsabel Martinez Ferrando
Department of Pharmacology and Molecular Sciences, Johns Hopkins School of Medicine, Baltimore, Maryland 21205, USA
Mol Cell Proteomics 11:355-69. 2012.... - Ca2+ signaling in mouse mesenteric small arteries: myogenic tone and adrenergic vasoconstrictionJoseph Zacharia
Dept of Physiology, Univ of Maryland, Baltimore, MD 21201, USA
Am J Physiol Heart Circ Physiol 292:H1523-32. 2007..alpha(1)-Adrenoceptor-induced vasoconstriction may be supported either by Ca(2+) waves or by steady elevation of cytoplasmic [Ca(2+)], depending on the amount of MT... - Signal propagation from membrane messengers to nuclear effectors revealed by reporters of phosphoinositide dynamics and Akt activityBharath Ananthanarayanan
Department of Pharmacology, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
Proc Natl Acad Sci U S A 102:15081-6. 2005..Thus, signal propagation from the lipid messengers at plasma membrane to the effectors in the nucleus is precisely controlled by kinases as well as lipid and protein phosphatases... - Spatiotemporally regulated protein kinase A activity is a critical regulator of growth factor-stimulated extracellular signal-regulated kinase signaling in PC12 cellsKatie J Herbst
Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
Mol Cell Biol 31:4063-75. 2011.... - Reporting from the field: genetically encoded fluorescent reporters uncover signaling dynamics in living biological systemsSohum Mehta
Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
Annu Rev Biochem 80:375-401. 2011.... - LRP6 mediates cAMP generation by G protein-coupled receptors through regulating the membrane targeting of Gα(s)Mei Wan
Department of Orthopaedic Surgery, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
Sci Signal 4:ra15. 2011..Thus, we suggest that the binding of Gα(s) to LRP6 is required to establish a functional GPCR-Gα(s)-AC signaling pathway for the production of cAMP, providing an additional regulatory component to the current GPCR-cAMP paradigm... - How does salt retention raise blood pressure?Mordecai P Blaustein
Department of Physiology, University of Maryland School of Medicine, 655 W Baltimore St, Baltimore, Maryland 21201, USA
Am J Physiol Regul Integr Comp Physiol 290:R514-23. 2006..Thus, endogenous ouabain, and vascular alpha2 Na+ pumps and NCX1, are critical links between salt and hypertension. New pharmacological agents that act on these molecular links have potential in the clinical management of hypertension... - Visualization of kinase activity with FRET-based activity biosensorsCHARLENE DEPRY
Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
Curr Protoc Mol Biol . 2010..This unit describes a general protocol for utilizing KARs to visualize kinase activity in living mammalian cells with fluorescence microscopy... - Visualization of PKA activity in plasma membrane microdomainsCHARLENE DEPRY
Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
Mol Biosyst 7:52-8. 2011.... - Widely accessible method for superresolution fluorescence imaging of living systemsPeter Dedecker
Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
Proc Natl Acad Sci U S A 109:10909-14. 2012..Furthermore, both 3D and multicolor imaging are readily achievable. Because of its ease of use and high performance, we anticipate that pcSOFI will prove an attractive approach for superresolution imaging... - A tunable FRET circuit for engineering fluorescent biosensorsMichael D Allen
Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, 725 N. Wolfe St, Baltimore, MD 21205, USA
Angew Chem Int Ed Engl 47:500-2. 2008 - Dynamic visualization of signal transduction in living cells: from second messengers to kinasesKatie J Herbst
Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, MD, USA
IUBMB Life 61:902-8. 2009.... - FRET-based biosensors for protein kinases: illuminating the kinomeJin Zhang
Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, 725 N Wolfe Street, Baltimore, MD 21205, USA
Mol Biosyst 3:759-65. 2007..Highlighted in this article is the design of genetically-encodable, FRET-based kinase biosensors with examples of their implementation to study kinase regulation in live biological contexts with high spatial and temporal resolution... - Left ventricular dysfunction and associated cellular injury in rats exposed to chronic intermittent hypoxiaLing Chen
Div of Pulmonary and Critical Care Medicine, Univ of Maryland, Baltimore, 685 West Baltimore St, MSTF 816, Baltimore, MD 21201, USA
J Appl Physiol 104:218-23. 2008..In conclusion, CIH-mediated LV global dysfunction is associated with myocyte hypertrophy and apoptosis at the cellular level... - Chemical rescue of a mutant enzyme in living cellsYingfeng Qiao
Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
Science 311:1293-7. 2006..Rescue of R388A cellular Src provided insights into the mitogen-activated protein kinase pathway. This chemical rescue approach will likely have many applications in cell signaling... - How cells process information: quantification of spatiotemporal signaling dynamicsAmbhighainath Ganesan
Department of Biomedical Engineering, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
Protein Sci 21:918-28. 2012..Finally, the authors illustrate the role of spatial compartmentalization in regulating cellular responses with examples of second-messenger signaling in cardiac myocytes... - Fluorescent indicators of cAMP and Epac activation reveal differential dynamics of cAMP signaling within discrete subcellular compartmentsLisa M DiPilato
Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
Proc Natl Acad Sci U S A 101:16513-8. 2004..Thus, cAMP dynamics and the activation of its effectors are precisely controlled spatiotemporally in vivo... - Analyzing protein kinase dynamics in living cells with FRET reportersQiang Ni
Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
Methods 40:279-86. 2006.... - Using FRET-based reporters to visualize subcellular dynamics of protein kinase A activityCHARLENE DEPRY
Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, MD, USA
Methods Mol Biol 756:285-94. 2011..This method details how to monitor real-time PKA activity dynamics in mammalian cells using fluorescence resonance energy transfer (FRET)-based reporters... - The cAMP-dependent protein kinase inhibitor H-89 attenuates the bioluminescence signal produced by Renilla LuciferaseKatie J Herbst
Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
PLoS ONE 4:e5642. 2009..These cell-based assays are often utilized to test the involvement of PKA-dependent processes by using H-89, a reversible competitive inhibitor of PKA... - Spatiotemporal analysis of differential Akt regulation in plasma membrane microdomainsXinxin Gao
Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
Mol Biol Cell 19:4366-73. 2008.... - Mechanistic distinction between activation and inhibition of (Na(+)+K(+))-ATPase-mediated Ca2+ influx in cardiomyocytesKai Y Xu
Department of Surgery, University of Maryland School of Medicine, Baltimore, MD 21201, USA
Biochem Biophys Res Commun 406:200-3. 2011.... - Fluorescent biosensors for real-time tracking of post-translational modification dynamicsNwe Nwe Aye-Han
Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
Curr Opin Chem Biol 13:392-7. 2009..In addition, we also provide discussions about various engineering strategies for overcoming potential challenges associated with the development and application of such biosensors... - Multiplexed visualization of dynamic signaling networks using genetically encoded fluorescent protein-based biosensorsCHARLENE DEPRY
Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, MD, USA
Pflugers Arch 465:373-81. 2013.... - Effect of oxygen on phosphodiesterases (PDE) 3 and 4 isoforms and PKA activity in the superior cervical gangliaAna Rita Nunes
Department of Pediatrics, Johns Hopkins University, Baltimore, MD 21287 3200, USA
Adv Exp Med Biol 758:287-94. 2012..The differential patterns of PDE regulation potentially represent subpopulations of ganglion cells with different physiological functions... - Galphas-biased beta2-adrenergic receptor signaling from restoring synchronous contraction in the failing heartKhalid Chakir
Division of Cardiology, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
Sci Transl Med 3:100ra88. 2011..These results identify a key pathway that is triggered by restoring contractile synchrony and that may represent a new therapeutic approach for a broad population of HF patients... - Localizer: fast, accurate, open-source, and modular software package for superresolution microscopyPeter Dedecker
The Johns Hopkins University School of Medicine, Department of Pharmacology and Molecular Sciences, 725 N Wolfe Street, Baltimore, Mayland 21205, USA
J Biomed Opt 17:126008. 2012..By dramatically improving the analysis performance and ensuring the easy addition of current and future enhancements, Localizer strongly improves the usability of superresolution imaging in a variety of biomedical studies... - Dynamic visualization of cellular signalingQiang Ni
Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, MD, 21205, USA
Adv Biochem Eng Biotechnol 119:79-97. 2010..Here we review recent advances in the development of such biosensors and some biological insights revealed by these biosensors in living cells, tissue, and organisms... - Adenosine diphosphate (ADP)-induced thromboxane A(2) generation in human platelets requires coordinated signaling through integrin alpha(IIb)beta(3) and ADP receptorsJianguo Jin
Department of Physiology, Temple University Medical School, Philadelphia, PA 19140, USA
Blood 99:193-8. 2002....