Janos Zempleni

Summary

Affiliation: University of Nebraska
Country: USA

Publications

  1. pmc Biotin requirements for DNA damage prevention
    Janos Zempleni
    Department of Nutrition and Health Sciences, University of Nebraska Lincoln, 316 Ruth Leverton Hall, Lincoln, NE 68583 0806, USA
    Mutat Res 733:58-60. 2012
  2. pmc The role of holocarboxylase synthetase in genome stability is mediated partly by epigenomic synergies between methylation and biotinylation events
    Janos Zempleni
    Department of Nutrition and Health Sciences, University of Nebraska Lincoln, Lincoln, NE, USA
    Epigenetics 6:892-4. 2011
  3. pmc Repression of transposable elements by histone biotinylation
    Janos Zempleni
    Department of Nutrition and Health Sciences, University of Nebraska at Lincoln, Lincoln, NE 68583, USA
    J Nutr 139:2389-92. 2009
  4. doi request reprint Biotin
    Janos Zempleni
    Department of Nutrition and Health Sciences, University of Nebraska Lincoln, 316 Ruth Leverton Hall, Lincoln, NE 68583 0806, USA
    Biofactors 35:36-46. 2009
  5. pmc Sodium-dependent multivitamin transporter gene is regulated at the chromatin level by histone biotinylation in human Jurkat lymphoblastoma cells
    Janos Zempleni
    Department of Nutrition and Health Sciences, University of Nebraska, Lincoln, NE 68583 0806, USA
    J Nutr 139:163-6. 2009
  6. ncbi request reprint Uptake, localization, and noncarboxylase roles of biotin
    Janos Zempleni
    Department of Nutrition and Health Sciences and Departments of Biochemistry and Animal Science, University of Nebraska at Lincoln, Nebraska 68583 0806, USA
    Annu Rev Nutr 25:175-96. 2005
  7. pmc Biotinylation of histones represses transposable elements in human and mouse cells and cell lines and in Drosophila melanogaster
    Yap Ching Chew
    Department of Nutrition and Health Sciences, University of Nebraska, Lincoln, NE 68583, USA
    J Nutr 138:2316-22. 2008
  8. ncbi request reprint Biotin supply affects rates of cell proliferation, biotinylation of carboxylases and histones, and expression of the gene encoding the sodium-dependent multivitamin transporter in JAr choriocarcinoma cells
    Sarah E R H Crisp
    Dept of Nutritional Science and Dietetics, University of Nebraska at Lincoln, 316 Ruth Leverton Hall, Lincoln, NE 68583 0806, USA
    Eur J Nutr 43:23-31. 2004
  9. pmc HepG2 cells develop signs of riboflavin deficiency within 4 days of culture in riboflavin-deficient medium
    Ricarda Werner
    Department of Nutrition and Health Sciences, University of Nebraska at Lincoln, Lincoln, NE 68583 0806, USA
    J Nutr Biochem 16:617-24. 2005
  10. pmc K16-biotinylated histone H4 is overrepresented in repeat regions and participates in the repression of transcriptionally competent genes in human Jurkat lymphoid cells
    Luisa Rios-Avila
    Department of Nutrition and Health Sciences, University of Nebraska Lincoln, Lincoln, NE 68583 0806, USA
    J Nutr Biochem 23:1559-64. 2012

Collaborators

Detail Information

Publications70

  1. pmc Biotin requirements for DNA damage prevention
    Janos Zempleni
    Department of Nutrition and Health Sciences, University of Nebraska Lincoln, 316 Ruth Leverton Hall, Lincoln, NE 68583 0806, USA
    Mutat Res 733:58-60. 2012
    ..These observations might offer a mechanistic explanation for some of the birth defects seen in biotin-deficient animal models...
  2. pmc The role of holocarboxylase synthetase in genome stability is mediated partly by epigenomic synergies between methylation and biotinylation events
    Janos Zempleni
    Department of Nutrition and Health Sciences, University of Nebraska Lincoln, Lincoln, NE, USA
    Epigenetics 6:892-4. 2011
    ..It appears that histone biotinylation depends on prior methylation of cytosines. De-repression of long terminal repeats in biotin- or HLCS-deficient cell cultures and organisms is associated with genome instability...
  3. pmc Repression of transposable elements by histone biotinylation
    Janos Zempleni
    Department of Nutrition and Health Sciences, University of Nebraska at Lincoln, Lincoln, NE 68583, USA
    J Nutr 139:2389-92. 2009
    ..Further, we hypothesize that this biotinylation promotes the subsequent dimethylation of lysine-9 in histone H3, resulting in an overall synergistic effect of 3 diet-dependent covalent modifications of histones in the repression of LTR...
  4. doi request reprint Biotin
    Janos Zempleni
    Department of Nutrition and Health Sciences, University of Nebraska Lincoln, 316 Ruth Leverton Hall, Lincoln, NE 68583 0806, USA
    Biofactors 35:36-46. 2009
    ..Possible effects of inadequate biotin intake, drug interactions, and inborn errors of metabolism are discussed, including putative effects on birth defects...
  5. pmc Sodium-dependent multivitamin transporter gene is regulated at the chromatin level by histone biotinylation in human Jurkat lymphoblastoma cells
    Janos Zempleni
    Department of Nutrition and Health Sciences, University of Nebraska, Lincoln, NE 68583 0806, USA
    J Nutr 139:163-6. 2009
    ....
  6. ncbi request reprint Uptake, localization, and noncarboxylase roles of biotin
    Janos Zempleni
    Department of Nutrition and Health Sciences and Departments of Biochemistry and Animal Science, University of Nebraska at Lincoln, Nebraska 68583 0806, USA
    Annu Rev Nutr 25:175-96. 2005
    ..Roles for biotin in cell signaling and chromatin structure are consistent with the notion that biotin has a unique significance in cell biology...
  7. pmc Biotinylation of histones represses transposable elements in human and mouse cells and cell lines and in Drosophila melanogaster
    Yap Ching Chew
    Department of Nutrition and Health Sciences, University of Nebraska, Lincoln, NE 68583, USA
    J Nutr 138:2316-22. 2008
    ..Collectively, this study reveals a novel diet-dependent epigenetic mechanism that could affect cancer risk...
  8. ncbi request reprint Biotin supply affects rates of cell proliferation, biotinylation of carboxylases and histones, and expression of the gene encoding the sodium-dependent multivitamin transporter in JAr choriocarcinoma cells
    Sarah E R H Crisp
    Dept of Nutritional Science and Dietetics, University of Nebraska at Lincoln, 316 Ruth Leverton Hall, Lincoln, NE 68583 0806, USA
    Eur J Nutr 43:23-31. 2004
    ..Placental transfer of nutrients and secretion of hormones is essential for normal fetal development...
  9. pmc HepG2 cells develop signs of riboflavin deficiency within 4 days of culture in riboflavin-deficient medium
    Ricarda Werner
    Department of Nutrition and Health Sciences, University of Nebraska at Lincoln, Lincoln, NE 68583 0806, USA
    J Nutr Biochem 16:617-24. 2005
    ..These studies are consistent with the hypothesis that HepG2 cells have a great demand for riboflavin and that cell stress develops rapidly if riboflavin supply is marginally low...
  10. pmc K16-biotinylated histone H4 is overrepresented in repeat regions and participates in the repression of transcriptionally competent genes in human Jurkat lymphoid cells
    Luisa Rios-Avila
    Department of Nutrition and Health Sciences, University of Nebraska Lincoln, Lincoln, NE 68583 0806, USA
    J Nutr Biochem 23:1559-64. 2012
    ..We conclude that H4K16bio is a real phenomenon and that this mark, like other biotinylation marks, is overrepresented in repressed loci where it marks HCS docking sites...
  11. pmc Biotinylation is a natural, albeit rare, modification of human histones
    Toshinobu Kuroishi
    Department of Nutrition and Health Sciences, University of Nebraska at Lincoln, Lincoln, NE 68583 0806, USA
    Mol Genet Metab 104:537-45. 2011
    ..In this model, docking of HCS in chromatin causes the occasional binding of biotin to histones as a tracer for HCS binding sites...
  12. ncbi request reprint Biotin deficiency stimulates survival pathways in human lymphoma cells exposed to antineoplastic drugs
    Jacob B Griffin
    Department of Nutrition and Health Sciences, University of Nebraska Lincoln, Lincoln, NE 68583 0806, USA
    J Nutr Biochem 16:96-103. 2005
    ..Collectively, these observations suggest that biotin deficiency may enhance resistance of cancer cells to antineoplastic agents...
  13. pmc Biotinylation of K12 in histone H4 decreases in response to DNA double-strand breaks in human JAr choriocarcinoma cells
    Nagarama Kothapalli
    Department of Biochemistry, University of Nebraska, Lincoln, NE, USA
    J Nutr 135:2337-42. 2005
    ..These studies suggest that the lack of biotinylation of K12 in histone H4 is an early signaling event in response to DSB...
  14. pmc Biotin supplementation decreases the expression of the SERCA3 gene (ATP2A3) in Jurkat cells, thus, triggering unfolded protein response
    Jacob B Griffin
    Department of Nutrition and Health Sciences, University of Nebraska at Lincoln, 68583 0806, USA
    J Nutr Biochem 17:272-81. 2006
    ..Collectively, this study suggests that supplements containing pharmacological doses of biotin may cause cell stress by impairing protein folding in the ER...
  15. pmc Lysine residues in N-terminal and C-terminal regions of human histone H2A are targets for biotinylation by biotinidase
    Yap Ching Chew
    Department of Nutrition and Health Sciences, University of Nebraska Lincoln, 68583 0806, USA
    J Nutr Biochem 17:225-33. 2006
    ..Collectively, these studies have identified five novel biotinylation sites in human histones; histone H2A is unique among histones in that its biotinylation sites include amino acid residues from the C-terminus...
  16. pmc K12-biotinylated histone H4 marks heterochromatin in human lymphoblastoma cells
    Gabriela Camporeale
    Department of Nutrition and Health Sciences, University of Nebraska Lincoln, Lincoln, NE 68583 0806, USA
    J Nutr Biochem 18:760-8. 2007
    ..These data are consistent with a novel role for biotin in chromatin structure and transcriptional activity of genes...
  17. pmc Feeding Drosophila a biotin-deficient diet for multiple generations increases stress resistance and lifespan and alters gene expression and histone biotinylation patterns
    Erin M Smith
    Department of Nutrition and Health Sciences, University of Nebraska, Lincoln, NE 68583 0806, USA
    J Nutr 137:2006-12. 2007
    ..Biotinylation of histones was barely detectable in biotin-deprived flies, suggesting that epigenetic events might have contributed to effects of biotin deprivation...
  18. pmc Prokaryotic BirA ligase biotinylates K4, K9, K18 and K23 in histone H3
    Keyna Kobza
    Departments of Nutrition and Health Sciences, University of Nebraska at Lincoln, Lincoln, NE, USA
    BMB Rep 41:310-5. 2008
    ..Moreover the biotinylation of histones by BirA ligase is consistent with the proposed role of human HCS in chromatin...
  19. pmc Novel histone biotinylation marks are enriched in repeat regions and participate in repression of transcriptionally competent genes
    Valerie Pestinger
    Department of Nutrition and Health Sciences, University of Nebraska Lincoln, Lincoln, NE 68583 0806, USA
    J Nutr Biochem 22:328-33. 2011
    ..Importantly, the enrichment of H3K9bio, H3K18bio and H4K8bio at genomic loci depended on the concentration of biotin in culture media at nutritionally relevant levels, suggesting a novel mechanism of gene regulation by biotin...
  20. pmc Biotin regulates the expression of holocarboxylase synthetase in the miR-539 pathway in HEK-293 cells
    Baolong Bao
    Department of Nutrition and Health Sciences, University of Nebraska at Lincoln, Lincoln, Nebraska 68583, USA
    J Nutr 140:1546-51. 2010
    ..Collectively, the results of this study suggest that miR-539 is among the factors sensing biotin and regulating HCS...
  21. pmc Cytosine methylation in miR-153 gene promoters increases the expression of holocarboxylase synthetase, thereby increasing the abundance of histone H4 biotinylation marks in HEK-293 human kidney cells
    Baolong Bao
    Department of Nutrition and Health Sciences, University of Nebraska at Lincoln, Lincoln, NE 68583, USA
    J Nutr Biochem 23:635-9. 2012
    ..Together, this study provides novel insights into the mechanisms of novel epigenetic synergies among folate-dependent methylation events, miR and histone biotinylation...
  22. ncbi request reprint Use of synthetic peptides for identifying biotinylation sites in human histones
    Gabriela Camporeale
    Department of Nutrition and Health Sciences, University of Nebraska at Lincoln, Lincoln, NE, USA
    Methods Mol Biol 418:139-48. 2008
    ..Amino acid substitutions (e.g., lysine-to-alanine substitutions) in synthetic peptides can be used to corroborate identification of biotinylation sites...
  23. pmc K4, K9 and K18 in human histone H3 are targets for biotinylation by biotinidase
    Keyna Kobza
    Department of Nutrition and Health Sciences, University of Nebraska Lincoln, Lincoln, NE 68583 0806, USA
    FEBS J 272:4249-59. 2005
    ..We speculate that this crosstalk provides a link to known roles for biotin in gene expression and cell proliferation...
  24. pmc K12-biotinylated histone H4 is enriched in telomeric repeats from human lung IMR-90 fibroblasts
    Subhashinee S K Wijeratne
    Department of Nutrition and Health Sciences, University of Nebraska at Lincoln, Lincoln, NE 68583 0806, USA
    J Nutr Biochem 21:310-6. 2010
    ..Collectively, these data suggest that H4K12bio is enriched in telomeric repeats and represents a novel epigenetic mark for cell senescence...
  25. pmc High-throughput immunoblotting identifies biotin-dependent signaling proteins in HepG2 hepatocarcinoma cells
    Rocio Rodriguez-Melendez
    Department of Nutrition and Health Sciences, University of Nebraska at Lincoln, Lincoln, NE, USA
    J Nutr 135:1659-66. 2005
    ....
  26. pmc Susceptibility to heat stress and aberrant gene expression patterns in holocarboxylase synthetase-deficient Drosophila melanogaster are caused by decreased biotinylation of histones, not of carboxylases
    Gabriela Camporeale
    Department of Nutrition and Health Sciences, University of Nebraska, Lincoln, NE 68583 0806, USA
    J Nutr 137:885-9. 2007
    ..We conclude that gene expression patterns and phenotypes in HCS-deficient flies in previous studies are caused by decreased biotinylation of histones rather than MCC...
  27. pmc Holocarboxylase synthetase is a chromatin protein and interacts directly with histone H3 to mediate biotinylation of K9 and K18
    Baolong Bao
    Department of Nutrition and Health Sciences, University of Nebraska at Lincoln, Lincoln, NE 68583, USA
    J Nutr Biochem 22:470-5. 2011
    ..We speculate that the targeting of HCS to distinct regions in human chromatin is mediated by DNA sequence, biotin, RNA, epigenetic marks or chromatin proteins...
  28. pmc Riboflavin deficiency causes protein and DNA damage in HepG2 cells, triggering arrest in G1 phase of the cell cycle
    Karoline C Manthey
    Department of Nutrition and Health Sciences, University of Nebraska at Lincoln, 68583 0806, USA, USA
    J Nutr Biochem 17:250-6. 2006
    ..We conclude that riboflavin deficiency is associated with oxidative damage to proteins and DNA in liver cells, leading to cell stress and G1 phase arrest...
  29. ncbi request reprint Biotin supplementation increases expression of the cytochrome P450 1B1 gene in Jurkat cells, increasing the occurrence of single-stranded DNA breaks
    Rocio Rodriguez-Melendez
    Departments of Nutrition and Health Sciences, University of Nebraska at Lincoln, Lincoln, NE, USA
    J Nutr 134:2222-8. 2004
    ..These studies provide evidence that transcription factors with an affinity for Sp1 sites mediate transcriptional activation of the CYP1B1 gene in biotin-supplemented T cells, increasing the occurrence of single-stranded DNA breaks...
  30. pmc Biotin requirements are lower in human Jurkat lymphoid cells but homeostatic mechanisms are similar to those of HepG2 liver cells
    Gaganpreet Kaur Mall
    Department of Nutrition and Health Sciences, University of Nebraska, Lincoln, NE 68583, USA
    J Nutr 140:1086-92. 2010
    ..The mechanisms of biotin homeostasis are qualitatively similar but quantitatively different in HepG2 and Jurkat cells; HCS, histone biotinylation, and biotin transporters play a role in homeostasis in both...
  31. pmc The polypeptide Syn67 interacts physically with human holocarboxylase synthetase, but is not a target for biotinylation
    Yousef I Hassan
    Department of Nutrition and Health Sciences, University of Nebraska at Lincoln, NE 68583 0806, USA
    Arch Biochem Biophys 495:35-41. 2010
    ..We identified a Syn67 docking site near the active pocket of HCS by in silico modeling and site-directed mutagenesis. Biotinylation of proteins by HCS is more specific than previously assumed...
  32. pmc Human holocarboxylase synthetase with a start site at methionine-58 is the predominant nuclear variant of this protein and has catalytic activity
    Baolong Bao
    Department of Nutrition and Health Sciences, University of Nebraska at Lincoln, Lincoln, NE, USA
    Biochem Biophys Res Commun 412:115-20. 2011
    ..We conclude this report by proposing a novel role for HLCS in epigenetic events, mediated by physical interactions between HLCS and other chromatin proteins as part of a larger multiprotein complex that mediates gene repression...
  33. ncbi request reprint The expression of genes encoding ribosomal subunits and eukaryotic translation initiation factor 5A depends on biotin and bisnorbiotin in HepG2 cells
    Rocio Rodriguez-Melendez
    Department of Nutrition and Health Sciences, University of Nebraska at Lincoln, 68583 0806, USA
    J Nutr Biochem 17:23-30. 2006
    ..Collectively, these data suggest that bisnorbiotin has biotin-like activities regarding gene expression, and that clusters of (bisnor)biotin-dependent genes include genes that play roles in translational activity...
  34. ncbi request reprint Clusters of biotin-responsive genes in human peripheral blood mononuclear cells
    Silke Wiedmann
    Department of Nutrition and Health Sciences, University of Nebraska at Lincoln, 316 Ruth Leverton Hall, Lincoln, NE 68583 0806, USA
    J Nutr Biochem 15:433-9. 2004
    ..This suggests that position effects play a role in biotin-dependent gene expression. Collectively, these findings suggest that the human genome contains clusters of biotin-dependent genes...
  35. ncbi request reprint Interleukin-2 receptor-gamma -dependent endocytosis depends on biotin in Jurkat cells
    Rocio Rodriguez-Melendez
    Department of Nutritional Science and Dietetics, University of Nebraska at Lincoln, Lincoln, Nebraska 68583, USA
    Am J Physiol Cell Physiol 284:C415-21. 2003
    ..Inhibition of endocytosis by genistein and overexpression of interleukin-2 receptor-gamma abolished the effect of biotin. These findings suggest that endocytosis of interleukin-2 depends on biotin...
  36. ncbi request reprint Monocarboxylate transporter 1 mediates biotin uptake in human peripheral blood mononuclear cells
    Rachel L Daberkow
    Departments of Nutritional Science and Dietetics, University of Nebraska at Lincoln, Lincoln, NE 68583, USA
    J Nutr 133:2703-6. 2003
    ..These findings are consistent with the hypothesis that MCT mediate biotin uptake in human lymphoid cells...
  37. ncbi request reprint Expression of oncogenes depends on biotin in human small cell lung cancer cells NCI-H69
    Sarah B Scheerger
    Department of Nutritional Science and Dietetics, University of Nebraska at Lincoln, Lincoln, NE 68583, USA
    Int J Vitam Nutr Res 73:461-7. 2003
    ..The clinical significance of effects of biotin supply on expression of oncogenes remains to be elaborated...
  38. ncbi request reprint Jurkat cells respond to biotin deficiency with increased nuclear translocation of NF-kappaB, mediating cell survival
    Rocio Rodriguez-Melendez
    Department of Nutrition and Health Science, University of Nebraska at Lincoln, Lincoln, NE 68583 0806, USA
    Int J Vitam Nutr Res 74:209-16. 2004
    ..These findings suggest that NF-kappaB mediates survival of biotin-deficient cells...
  39. doi request reprint Identification and assessment of markers of biotin status in healthy adults
    Wei Kay Eng
    Department of Nutrition, University of Nebraska Lincoln, Lincoln, NE 68583 0806, USA
    Br J Nutr 110:321-9. 2013
    ..Generally, datasets were characterised by variations that exceeded those seen in studies in cell cultures. We conclude that holo-MCC and holo-PCC are the most reliable, single markers of biotin status tested in the present study...
  40. ncbi request reprint Exposure to UV light causes increased biotinylation of histones in Jurkat cells
    Dorothea M Peters
    Department of Nutritional Science and Dietetics, University of Nebraska at Lincoln, Lincoln 68583, USA
    Am J Physiol Cell Physiol 283:C878-84. 2002
    ..In summary, this study provided evidence that increased biotinylation of histones in DNA-damaged cells might either be a side product of carboxylase degradation or a step during apoptosis...
  41. ncbi request reprint Regulation of gene expression by biotin (review)
    Rocio Rodriguez-Melendez
    Department of Nutritional Science and Dietetics, University of Nebraska at Lincoln, NE 68583, USA
    J Nutr Biochem 14:680-90. 2003
    ..These new insights into biotin-dependent gene expression are likely to lead to a better understanding of roles for biotin in cell biology and fetal development...
  42. pmc Biological functions of biotinylated histones
    Nagarama Kothapalli
    Department of Nutrition and Health Sciences, University of Nebraska at Lincoln, Lincoln, NE 68583 0806, USA
    J Nutr Biochem 16:446-8. 2005
    ..Evidence was provided that biotinylation of histones plays a role in cell proliferation, gene silencing and cellular response to DNA damage...
  43. ncbi request reprint Diaminobiotin and desthiobiotin have biotin-like activities in Jurkat cells
    Rocio Rodriguez-Melendez
    Department of Nutritional Science and Dietetics, University of Nebraska at Lincoln, Lincoln, NE, USA
    J Nutr 133:1259-64. 2003
    ..It is not known whether naturally occurring biotin metabolites also have biotin-like activities...
  44. pmc An avidin-based assay for histone debiotinylase activity in human cell nuclei
    Yap Ching Chew
    Department of Nutrition and Health Sciences, University of Nebraska at Lincoln, Lincoln, NE 68583 0806, USA
    J Nutr Biochem 18:475-81. 2007
    ..This assay proved useful in monitoring activities of putative histone debiotinylases during their partial purification from cells. Collectively, this assay is a useful tool for investigating histone debiotinylases in human tissues...
  45. pmc Riboflavin deficiency impairs oxidative folding and secretion of apolipoprotein B-100 in HepG2 cells, triggering stress response systems
    Karoline C Manthey
    Department of Nutrition and Health Sciences, University of Nebraska at Lincoln, 68583, USA
    J Nutr 135:978-82. 2005
    ..Decreased secretion of apoB in riboflavin-deficient cells might interfere with lipid homeostasis in vivo...
  46. pmc Biotinyl-methyl 4-(amidomethyl)benzoate is a competitive inhibitor of human biotinidase
    Keyna A Kobza
    Department of Nutrition and Health Sciences, University of Nebraska Lincoln, Lincoln, NE 68583 0806, USA
    J Nutr Biochem 19:826-32. 2008
    ..Finally, biotinyl-methyl 4-(amidomethyl)benzoate did not affect biotin transport in human cells, suggesting specificity in regard to biotin-related processes...
  47. pmc Drosophila melanogaster holocarboxylase synthetase is a chromosomal protein required for normal histone biotinylation, gene transcription patterns, lifespan, and heat tolerance
    Gabriela Camporeale
    Department of Nutrition and Health Sciences, University of Nebraska, Lincoln, NE 68583 0806, USA
    J Nutr 136:2735-42. 2006
    ....
  48. ncbi request reprint Roles for nutrients in epigenetic events
    Anna M Oommen
    Department of Nutrition and Health Sciences, University of Nebraska at Lincoln, Lincoln, NE 68583 0806, USA
    J Nutr Biochem 16:74-7. 2005
    ....
  49. ncbi request reprint The nuclear abundance of transcription factors Sp1 and Sp3 depends on biotin in Jurkat cells
    Jacob B Griffin
    Department of Nutritional Science and Dietetics, University of Nebraska at Lincoln, Lincoln, NE, USA
    J Nutr 133:3409-15. 2003
    ..This study provides evidence that some effects of biotin on gene expression might be mediated by the nuclear abundance of Sp1 and Sp3...
  50. ncbi request reprint Synthesis of a rabbit polyclonal antibody to the human sodium-dependent multivitamin transporter
    Jacob B Griffin
    Department of Nutritional Sciences and Dietetics, University of Nebraska at Lincoln, 316 Ruth Leverton Hall, Lincoln, NE 68583 0806, USA
    Int J Vitam Nutr Res 72:195-8. 2002
    ..Using this antibody, SMVT has been identified in human peripheral blood mononuclear cells, Caco-2 cells, and HepG2 cells. Moreover, we observed that cells respond to proliferation with increased synthesis of SMVT...
  51. pmc Holocarboxylase synthetase regulates expression of biotin transporters by chromatin remodeling events at the SMVT locus
    Michael Gralla
    Department of Nutrition and Health Sciences, University of Nebraska at Lincoln, Lincoln, NE 68583 0806, USA
    J Nutr Biochem 19:400-8. 2008
    ....
  52. ncbi request reprint Biotinidase catalyzes debiotinylation of histones
    Tyrie D Ballard
    Department of Nutritional Sciences and Dietetics, University of Nebraska at Lincoln, 68583 0806, USA
    Eur J Nutr 41:78-84. 2002
    ....
  53. ncbi request reprint K8 and K12 are biotinylated in human histone H4
    Gabriela Camporeale
    Department of Nutrition and Health Sciences, University of Nebraska at Lincoln, Lincoln, NE 68583 0806, USA
    Eur J Biochem 271:2257-63. 2004
    ....
  54. ncbi request reprint Oxidative folding of interleukin-2 is impaired in flavin-deficient jurkat cells, causing intracellular accumulation of interleukin-2 and increased expression of stress response genes
    Gabriela Camporeale
    Department of Nutritional Science and Dietetics, University of Nebraska at Lincoln, USA
    J Nutr 133:668-72. 2003
    ..These findings are consistent with the hypothesis that flavin deficiency may cause cellular stress by accumulation of unfolded proteins in human cells...
  55. doi request reprint A novel, enigmatic histone modification: biotinylation of histones by holocarboxylase synthetase
    Yousef I Hassan
    Department of Nutrition and Health Sciences, University of Nebraska Lincoln, Lincoln, Nebraska 68583 0806, USA
    Nutr Rev 66:721-5. 2008
    ..Decreased histone biotinylation decreases life span and stress resistance in Drosophila. Low enrichment of biotinylated histones at transposable elements impairs repression of these elements...
  56. ncbi request reprint Biotin deficiency decreases life span and fertility but increases stress resistance in Drosophila melanogaster
    Anette Landenberger
    Department of Nutrition and Health Sciences, University of Nebraska at Lincoln, 316 Ruth Leverton Hall, Lincoln, NE 68583 0806, USA
    J Nutr Biochem 15:591-600. 2004
    ..These findings are consistent with the hypothesis that biotin affects life span, stress resistance, and fertility in fruit flies...
  57. pmc Epigenetic regulation of chromatin structure and gene function by biotin
    Yousef I Hassan
    Department of Nutrition and Health Sciences, University of Nebraska at Lincoln, Lincoln, NE, USA
    J Nutr 136:1763-5. 2006
    ..We propose that some effects of biotin deficiency can be attributed to abnormal chromatin structures...
  58. pmc Genomic implications of H(2)O (2) for cell proliferation and growth of Caco-2 cells
    Theresa A Herring
    Department of Nutrition and Health Sciences, University of Nebraska Lincoln, Lincoln, Nebraska 68583, USA
    Dig Dis Sci 52:3005-15. 2007
    ....
  59. pmc Nitric oxide signaling depends on biotin in Jurkat human lymphoma cells
    Rocio Rodriguez-Melendez
    Department of Nutrition and Health Sciences, University of Nebraska, Lincoln, NE 68583, USA
    J Nutr 139:429-33. 2009
    ..Collectively, the results of this study are consistent with the hypothesis that biotin-dependent cGMP signaling in human lymphoid cells is mediated by NO...
  60. pmc N- and C-terminal domains in human holocarboxylase synthetase participate in substrate recognition
    Yousef I Hassan
    Department of Nutrition and Health Sciences, University of Nebraska at Lincoln, 316 Ruth Leverton Hall, Lincoln, NE 68583 0806, USA
    Mol Genet Metab 96:183-8. 2009
    ..In silico predictions were consistent with observations from Y2H assays and yeast rescue experiments, and suggested docking of p67 near Arg508 and Ser515 within the central domain of HCS...
  61. pmc Online tools for bioinformatics analyses in nutrition sciences
    Sridhar A Malkaram
    Department of Nutrition and Health Sciences, University of Nebraska, Lincoln, Nebraska, USA
    Adv Nutr 3:654-65. 2012
    ....
  62. pmc Effects of single-nucleotide polymorphisms in the human holocarboxylase synthetase gene on enzyme catalysis
    Shingo Esaki
    Department of Nutrition and Health Sciences, University of Nebraska Lincoln, Lincoln, NE 68583, USA
    Eur J Hum Genet 20:428-33. 2012
    ..The V96 L SNP did not alter enzyme kinetics. Our findings suggest that individuals with HLCS SNPs may benefit from supplemental biotin, yet to different extents depending on the genotype...
  63. ncbi request reprint Biotin supplementation increases expression of genes encoding interferon-gamma, interleukin-1beta, and 3-methylcrotonyl-CoA carboxylase, and decreases expression of the gene encoding interleukin-4 in human peripheral blood mononuclear cells
    Silke Wiedmann
    Department of Nutritional Science and Dietetics, University of Nebraska at Lincoln, 68583, USA
    J Nutr 133:716-9. 2003
    ..These data suggest that biotin supplementation affects gene expression in human immune cells. Effects of biotin on gene expression are likely to modulate the response of immune cells to antigens...
  64. pmc A 96-well plate assay for high-throughput analysis of holocarboxylase synthetase activity
    Luisa Rios-Avila
    Department of Nutrition and Health Sciences, University of Nebraska Lincoln, NE 68583 0806, USA
    Clin Chim Acta 412:735-9. 2011
    ..HCS null mammals are not viable whereas HCS deficiency is linked to developmental delays in humans and phenotypes such as short life span and low stress resistance in Drosophila...
  65. ncbi request reprint Biotin supply affects expression of biotin transporters, biotinylation of carboxylases and metabolism of interleukin-2 in Jurkat cells
    Karoline C Manthey
    Department of Nutritional Science and Dietetics, University of Nebraska at Lincoln, Lincoln, NE 68583 0806, USA
    J Nutr 132:887-92. 2002
    ..The physiological significance of effects of biotin status on metabolism of interleukin-2 remains to be elaborated...
  66. pmc Identification of holocarboxylase synthetase chromatin binding sites in human mammary cell lines using the DNA adenine methyltransferase identification technology
    Dipika Singh
    Department of Biological Systems Engineering, University of Nebraska Lincoln, Lincoln, NE 68583, USA
    Anal Biochem 413:55-9. 2011
    ..We conclude that DamID is a useful technology to map HCS binding sites in human chromatin and propose that the entire set of HCS binding sites could be mapped by combining DamID with microarray technology...
  67. pmc Development of an internet based system for modeling biotin metabolism using Bayesian networks
    Jinglei Zhou
    Department of Statistics, University of Nebraska Lincoln, Lincoln, NE 68583, USA
    Comput Methods Programs Biomed 104:254-9. 2011
    ..This program enables convenient in silico experiments regarding biotin metabolism, which can help researchers design future experiments while new data can be continuously incorporated...
  68. ncbi request reprint Biotin availability regulates expression of the sodium-dependent multivitamin transporter and the rate of biotin uptake in HepG2 cells
    Diana Pacheco-Alvarez
    Department of Biología Molecular y Biotecnología, Instituto de Investigaciones Biomedicas, Universidad Nacional Autonoma de Mexico, Mexico D F 04510, Mexico
    Mol Genet Metab 85:301-7. 2005
    ..Our results support the proposed role of SMVT in the altruistic regulation of biotin utilization in liver cells that has been associated with sparing biotin depletion of the brain...
  69. pmc Biotin dependency due to a defect in biotin transport
    Rebecca Mardach
    Regional Metabolic Service, Kaiser Permanente, Los Angeles, California, USA
    J Clin Invest 109:1617-23. 2002
    ..These results provide evidence for a novel genetic defect in biotin transport. This child is the first known with this defect, which should now be included in the identified causes of biotin dependency...
  70. pmc Biotin uptake into human peripheral blood mononuclear cells increases early in the cell cycle, increasing carboxylase activities
    J Steven Stanley
    Arkansas Children s Hospital Research Institute, Little Rock, AR, USA
    J Nutr 132:1854-9. 2002
    ..In summary, these findings suggest that PBMC respond to proliferation with increased biotin uptake early in the cell cycle, and that biotin is utilized to increase activities of two of the four biotin-requiring carboxylases...

Research Grants15

  1. Biotin Deficiency Impairs Silencing of Repeat Regions & Retrotransposons
    Janos Zempleni; Fiscal Year: 2010
    ..This proposal lays the groundwork for decreasing the incidence of retrotransposon-associated diseases in groups at risk for developing biotin deficiency such as pregnant women and their fetuses. ..
  2. Biotin sensing and chromatin remodeling by holocarboxylase synthetase
    Janos Zempleni; Fiscal Year: 2010
    ..Previous studies suggest that biotinylation of histones plays a critical role in gene regulation and genomic stability, thereby decreasing the risk for chromosomal abnormalities and cancer. ..
  3. Biotin sensing and chromatin remodeling by holocarboxylase synthetase
    Janos Zempleni; Fiscal Year: 2009
    ..Previous studies suggest that biotinylation of histones plays a critical role in gene regulation and genomic stability, thereby decreasing the risk for chromosomal abnormalities and cancer. ..
  4. Epigenetic effects of biotin on activation of endogenous viral sequences
    Janos Zempleni; Fiscal Year: 2007
    ..These studies are likely to identify means by which manipulation of the environmental factor "diet" increases genomic stability and decreases cancer risk. ..
  5. Vitamin-dependent modifications of histones
    Janos Zempleni; Fiscal Year: 2007
    ..The potential role of biotin in these processes establishes a link between cellular biotin homeostasis and DNA mutation and abnormal proliferation such as in cancer cells. ..
  6. Essential role of biotin in cell proliferation
    Janos Zempleni; Fiscal Year: 2004
    ..The basic knowledge generated may well be relevant to teratogenesis and immune dysfunction caused by biotin deficiency. ..
  7. Biotin sensing and chromatin remodeling by holocarboxylase synthetase
    Janos Zempleni; Fiscal Year: 2010
    ..Previous studies suggest that biotinylation of histones plays a critical role in gene regulation and genomic stability, thereby decreasing the risk for chromosomal abnormalities and cancer. ..