Wolfram R Zückert

Summary

Affiliation: University of Kansas Medical Center
Country: USA

Publications

  1. pmc Cross-species surface display of functional spirochetal lipoproteins by recombinant Borrelia burgdorferi
    Wolfram R Zückert
    Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, Kansas 66160, USA
    Infect Immun 72:1463-9. 2004
  2. pmc Secretion of bacterial lipoproteins: through the cytoplasmic membrane, the periplasm and beyond
    Wolfram R Zückert
    University of Kansas School of Medicine, Department of Microbiology, Molecular Genetics and Immunology, Kansas City, KS, United States Electronic address
    Biochim Biophys Acta 1843:1509-16. 2014
  3. pmc A call to order at the spirochaetal host-pathogen interface
    Wolfram R Zückert
    Department of Microbiology, Molecular Genetics and Immunology, University of Kansas School of Medicine, 3901 Rainbow Boulevard, Kansas City, KS 66160, USA
    Mol Microbiol 89:207-11. 2013
  4. ncbi request reprint Borrelia burgdorferi lipoproteins are secreted to the outer surface by default
    Ryan J Schulze
    Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Mail Stop 3029, 3901 Rainbow Boulevard, Kansas City, 66160, USA
    Mol Microbiol 59:1473-84. 2006
  5. pmc Probing the Borrelia burgdorferi surface lipoprotein secretion pathway using a conditionally folding protein domain
    Shiyong Chen
    Department of Microbiology, Molecular Genetics, and Immunology, University of Kansas Medical Center, 3901 Rainbow Boulevard, Kansas City, KS 66160, USA
    J Bacteriol 193:6724-32. 2011
  6. pmc Translocation of Borrelia burgdorferi surface lipoprotein OspA through the outer membrane requires an unfolded conformation and can initiate at the C-terminus
    Ryan J Schulze
    Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Mail Stop 3029, 3901 Rainbow Boulevard, Kansas City, KS 66160, USA
    Mol Microbiol 76:1266-78. 2010
  7. pmc Determination of Borrelia surface lipoprotein anchor topology by surface proteolysis
    Shiyong Chen
    Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Mail Stop 3029, 3901 Rainbow Boulevard, Kansas City, KS 66160, USA
    J Bacteriol 193:6379-83. 2011
  8. pmc Surface localization determinants of Borrelia OspC/Vsp family lipoproteins
    Ozan S Kumru
    University of Kansas Medical Center, Department of Microbiology, Molecular Genetics and Immunology, Mail Stop 3029, 3025 Wahl Hall West, 3901 Rainbow Boulevard, Kansas City, KS 66160, USA
    J Bacteriol 193:2814-25. 2011
  9. pmc BpaB and EbfC DNA-binding proteins regulate production of the Lyme disease spirochete's infection-associated Erp surface proteins
    Brandon L Jutras
    Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky College of Medicine, Lexington, Kentucky, USA
    J Bacteriol 194:778-86. 2012
  10. pmc Development of a single-plasmid-based regulatable gene expression system for Borrelia burgdorferi
    Christine R Whetstine
    Department of Microbiology, University of Kansas Medical Center, Mail Stop 3019, 3025 Wahl Hall West, 3901 Rainbow Boulevard, Kansas City, KS 66160 7420, USA
    Appl Environ Microbiol 75:6553-8. 2009

Research Grants

  1. Lipoprotein Transport in Borrelia Spirochetes
    WOLFRAM R ZUECKERT; Fiscal Year: 2010
  2. Structure-Function of Borrelia Surface Lipoproteins
    WOLFRAM ZUECKERT; Fiscal Year: 2005
  3. Lipoprotein Transport in Borrelia Spirochetes
    WOLFRAM ZUECKERT; Fiscal Year: 2006
  4. Lipoprotein Transport in Borrelia Spirochetes
    WOLFRAM ZUECKERT; Fiscal Year: 2007
  5. Lipoprotein Transport in Borrelia Spirochetes
    WOLFRAM ZUECKERT; Fiscal Year: 2009

Collaborators

Detail Information

Publications14

  1. pmc Cross-species surface display of functional spirochetal lipoproteins by recombinant Borrelia burgdorferi
    Wolfram R Zückert
    Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, Kansas 66160, USA
    Infect Immun 72:1463-9. 2004
    ..They also validate the use of recombinant B. burgdorferi in studies of surface lipoprotein structure-function and the biogenesis of spirochete membranes...
  2. pmc Secretion of bacterial lipoproteins: through the cytoplasmic membrane, the periplasm and beyond
    Wolfram R Zückert
    University of Kansas School of Medicine, Department of Microbiology, Molecular Genetics and Immunology, Kansas City, KS, United States Electronic address
    Biochim Biophys Acta 1843:1509-16. 2014
    ..This article is part of a Special Issue entitled: Protein trafficking and secretion in bacteria. Guest Editors: Anastassios Economou and Ross Dalbey. ..
  3. pmc A call to order at the spirochaetal host-pathogen interface
    Wolfram R Zückert
    Department of Microbiology, Molecular Genetics and Immunology, University of Kansas School of Medicine, 3901 Rainbow Boulevard, Kansas City, KS 66160, USA
    Mol Microbiol 89:207-11. 2013
    ..These data reinforce the notion that OspC plays a unique role during initial infection while the antigenically variant VlsE proteins allow for persistence in the mammalian host. ..
  4. ncbi request reprint Borrelia burgdorferi lipoproteins are secreted to the outer surface by default
    Ryan J Schulze
    Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Mail Stop 3029, 3901 Rainbow Boulevard, Kansas City, 66160, USA
    Mol Microbiol 59:1473-84. 2006
    ..Together, these data indicate that Borrelia lipoproteins are targeted to the bacterial surface by default, but can be retained in the periplasm by sequence-specific signals...
  5. pmc Probing the Borrelia burgdorferi surface lipoprotein secretion pathway using a conditionally folding protein domain
    Shiyong Chen
    Department of Microbiology, Molecular Genetics, and Immunology, University of Kansas Medical Center, 3901 Rainbow Boulevard, Kansas City, KS 66160, USA
    J Bacteriol 193:6724-32. 2011
    ....
  6. pmc Translocation of Borrelia burgdorferi surface lipoprotein OspA through the outer membrane requires an unfolded conformation and can initiate at the C-terminus
    Ryan J Schulze
    Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Mail Stop 3029, 3901 Rainbow Boulevard, Kansas City, KS 66160, USA
    Mol Microbiol 76:1266-78. 2010
    ..Furthermore, they suggest that OspA emerges tail-first on the bacterial surface, yet independent of a specific C-terminal targeting peptide sequence...
  7. pmc Determination of Borrelia surface lipoprotein anchor topology by surface proteolysis
    Shiyong Chen
    Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Mail Stop 3029, 3901 Rainbow Boulevard, Kansas City, KS 66160, USA
    J Bacteriol 193:6379-83. 2011
    ..Our findings with both wild-type and mutant proteins are only compatible with the anchoring of these surface lipoproteins in the outer leaflet of the outer spirochetal membrane...
  8. pmc Surface localization determinants of Borrelia OspC/Vsp family lipoproteins
    Ozan S Kumru
    University of Kansas Medical Center, Department of Microbiology, Molecular Genetics and Immunology, Mail Stop 3029, 3025 Wahl Hall West, 3901 Rainbow Boulevard, Kansas City, KS 66160, USA
    J Bacteriol 193:2814-25. 2011
    ....
  9. pmc BpaB and EbfC DNA-binding proteins regulate production of the Lyme disease spirochete's infection-associated Erp surface proteins
    Brandon L Jutras
    Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky College of Medicine, Lexington, Kentucky, USA
    J Bacteriol 194:778-86. 2012
    ..Additionally, a combination of in vivo and in vitro methods demonstrated that BpaB functions as a repressor of erp transcription, while EbfC functions as an antirepressor...
  10. pmc Development of a single-plasmid-based regulatable gene expression system for Borrelia burgdorferi
    Christine R Whetstine
    Department of Microbiology, University of Kansas Medical Center, Mail Stop 3019, 3025 Wahl Hall West, 3901 Rainbow Boulevard, Kansas City, KS 66160 7420, USA
    Appl Environ Microbiol 75:6553-8. 2009
    ..Since target protein, operator, and repressor are carried by a single plasmid, the system's application is independent of a particular strain background...
  11. pmc Specificity and role of the Borrelia burgdorferi CtpA protease in outer membrane protein processing
    Ozan S Kumru
    Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, Kansas 66160, USA
    J Bacteriol 193:5759-65. 2011
    ....
  12. pmc Development and validation of a FACS-based lipoprotein localization screen in the Lyme disease spirochete Borrelia burgdorferi
    Ozan S Kumru
    Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Mail Stop 3029, 3901 Rainbow Boulevard, Kansas City, KS 66160, USA
    BMC Microbiol 10:277. 2010
    ..To build on these findings and to complement the prior targeted mutagenesis approach, we set out to develop a screen to probe a random mutagenesis expression library for mutants expressing differentially localized lipoproteins...
  13. ncbi request reprint Regulated synthesis of the Borrelia burgdorferi inner-membrane lipoprotein IpLA7 (P22, P22-A) during the Lyme disease spirochaete's mammal-tick infectious cycle
    Kate von Lackum
    Department of Microbiology, Immunology and Molecular Genetics, University of Kentucky College of Medicine, Lexington, KY 40536, USA
    Microbiology 153:1361-71. 2007
    ....
  14. ncbi request reprint Laboratory maintenance of Borrelia burgdorferi
    Wolfram R Zückert
    University of Kansas Medical Center, Kansas City, Kansas, USA
    Curr Protoc Microbiol . 2007
    ..It supplies a detailed recipe for BSK-II, as well as protocols for standard liquid culture, the generation of frozen Borrelia stocks, and the plating of B. burgdorferi cells in solid BSK-II medium...

Research Grants6

  1. Lipoprotein Transport in Borrelia Spirochetes
    WOLFRAM R ZUECKERT; Fiscal Year: 2010
    ....
  2. Structure-Function of Borrelia Surface Lipoproteins
    WOLFRAM ZUECKERT; Fiscal Year: 2005
    ..Furthermore, they will yield important clues for the design of future intervention strategies. ..
  3. Lipoprotein Transport in Borrelia Spirochetes
    WOLFRAM ZUECKERT; Fiscal Year: 2006
    ....
  4. Lipoprotein Transport in Borrelia Spirochetes
    WOLFRAM ZUECKERT; Fiscal Year: 2007
    ....
  5. Lipoprotein Transport in Borrelia Spirochetes
    WOLFRAM ZUECKERT; Fiscal Year: 2009
    ....