Phillip Zamore

Summary

Affiliation: University of Massachusetts Medical School
Country: USA

Publications

  1. pmc Strand-specific libraries for high throughput RNA sequencing (RNA-Seq) prepared without poly(A) selection
    Zhao Zhang
    Biochemistry and Molecular Pharmacology, and Howard Hughes Medical Institute, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA, 01605, USA
    Silence 3:9. 2012
  2. pmc RNA: methods and protocols - a new series
    Phillip D Zamore
    Howard Hughes Medical Institute and Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA, 01605, USA
    Silence 3:7. 2012
  3. pmc Normal microRNA maturation and germ-line stem cell maintenance requires Loquacious, a double-stranded RNA-binding domain protein
    Klaus Forstemann
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts, USA
    PLoS Biol 3:e236. 2005
  4. pmc A 5'-uridine amplifies miRNA/miRNA* asymmetry in Drosophila by promoting RNA-induced silencing complex formation
    Herve Seitz
    Laboratoire de Biologie Moleculaire Eucaryote, 118 route de Narbonne, Université Toulouse III Paul Sabatier UPS, F 31000 Toulouse, France
    Silence 2:4. 2011
  5. ncbi request reprint RNAi: double-stranded RNA directs the ATP-dependent cleavage of mRNA at 21 to 23 nucleotide intervals
    P D Zamore
    Department of Biochemistry and Molecular Biology, University of Massachusetts Medical School, Worcester 01655, USA
    Cell 101:25-33. 2000
  6. ncbi request reprint Ribo-gnome: the big world of small RNAs
    Phillip D Zamore
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Science 309:1519-24. 2005
  7. ncbi request reprint Ancient pathways programmed by small RNAs
    Phillip D Zamore
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Lazare Research Building, Room 825, 364 Plantation Street, Worcester, MA 01605, USA
    Science 296:1265-9. 2002
  8. ncbi request reprint RNA interference: listening to the sound of silence
    P D Zamore
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, S6 240, 55 Lake Avenue North, Worcester, MA 01655, USA
    Nat Struct Biol 8:746-50. 2001
  9. ncbi request reprint RNA interference: big applause for silencing in Stockholm
    Phillip D Zamore
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Cell 127:1083-6. 2006
  10. ncbi request reprint A microRNA in a multiple-turnover RNAi enzyme complex
    Gyorgy Hutvagner
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Lazare Research Building, Room 825, 364 Plantation Street, Worcester, MA 01605, USA
    Science 297:2056-60. 2002

Research Grants

Collaborators

Detail Information

Publications50

  1. pmc Strand-specific libraries for high throughput RNA sequencing (RNA-Seq) prepared without poly(A) selection
    Zhao Zhang
    Biochemistry and Molecular Pharmacology, and Howard Hughes Medical Institute, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA, 01605, USA
    Silence 3:9. 2012
    ..abstract:..
  2. pmc RNA: methods and protocols - a new series
    Phillip D Zamore
    Howard Hughes Medical Institute and Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA, 01605, USA
    Silence 3:7. 2012
    ..ABSTRACT: This month, Silence launches a new series on methods and protocols to study silencing pathways and analyze nucleic acids and proteins...
  3. pmc Normal microRNA maturation and germ-line stem cell maintenance requires Loquacious, a double-stranded RNA-binding domain protein
    Klaus Forstemann
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts, USA
    PLoS Biol 3:e236. 2005
    ..Thus, every known Drosophila RNase-III endonuclease is paired with a dsRBD protein that facilitates its function in small RNA biogenesis...
  4. pmc A 5'-uridine amplifies miRNA/miRNA* asymmetry in Drosophila by promoting RNA-induced silencing complex formation
    Herve Seitz
    Laboratoire de Biologie Moleculaire Eucaryote, 118 route de Narbonne, Université Toulouse III Paul Sabatier UPS, F 31000 Toulouse, France
    Silence 2:4. 2011
    ..abstract:..
  5. ncbi request reprint RNAi: double-stranded RNA directs the ATP-dependent cleavage of mRNA at 21 to 23 nucleotide intervals
    P D Zamore
    Department of Biochemistry and Molecular Biology, University of Massachusetts Medical School, Worcester 01655, USA
    Cell 101:25-33. 2000
    ..Cleavage occurs at sites 21-23 nucleotides apart, the same interval observed for the dsRNA itself, suggesting that the 21-23 nucleotide fragments from the dsRNA are guiding mRNA cleavage...
  6. ncbi request reprint Ribo-gnome: the big world of small RNAs
    Phillip D Zamore
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Science 309:1519-24. 2005
    ....
  7. ncbi request reprint Ancient pathways programmed by small RNAs
    Phillip D Zamore
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Lazare Research Building, Room 825, 364 Plantation Street, Worcester, MA 01605, USA
    Science 296:1265-9. 2002
    ....
  8. ncbi request reprint RNA interference: listening to the sound of silence
    P D Zamore
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, S6 240, 55 Lake Avenue North, Worcester, MA 01655, USA
    Nat Struct Biol 8:746-50. 2001
    ..The discovery of RNAi has changed our understanding of how cells guard their genomes, led to the development of new strategies for blocking gene function, and may yet yield RNA-based drugs to treat human disease...
  9. ncbi request reprint RNA interference: big applause for silencing in Stockholm
    Phillip D Zamore
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Cell 127:1083-6. 2006
    ..For this landmark discovery, Mello and Fire are honored with this year's Nobel Prize in Physiology or Medicine...
  10. ncbi request reprint A microRNA in a multiple-turnover RNAi enzyme complex
    Gyorgy Hutvagner
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Lazare Research Building, Room 825, 364 Plantation Street, Worcester, MA 01605, USA
    Science 297:2056-60. 2002
    ..Each let-7-containing complex directs multiple rounds of RNA cleavage, which explains the remarkable efficiency of the RNAi pathway in human cells...
  11. pmc A biochemical framework for RNA silencing in plants
    Guiliang Tang
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    Genes Dev 17:49-63. 2003
    ..Thus, endonuclease complexes guided by small RNAs are a common feature of RNA silencing in both animals and plants...
  12. ncbi request reprint Passenger-strand cleavage facilitates assembly of siRNA into Ago2-containing RNAi enzyme complexes
    Christian Matranga
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    Cell 123:607-20. 2005
    ..For siRNAs, virtually all RISC is assembled through this cleavage-assisted mechanism. In contrast, passenger-strand cleavage is not important for the incorporation of miRNAs that derive from mismatched duplexes...
  13. ncbi request reprint Asymmetry in the assembly of the RNAi enzyme complex
    Dianne S Schwarz
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Lazare Research Building, 364 Plantation Street, Worcester, MA 01605, USA
    Cell 115:199-208. 2003
    ..Thus, the common step of RISC assembly is an unexpected source of asymmetry for both siRNA function and miRNA biogenesis...
  14. ncbi request reprint Biochemical dissection of RNA silencing in plants
    Guiliang Tang
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA, USA
    Methods Mol Biol 257:223-44. 2004
    ..These comprehensive protocols should prove useful in the further dissection of the plant RNA silencing pathway, as well as for the validation of the predicted targets of endogenous plant microRNAs...
  15. pmc Sequence-specific inhibition of small RNA function
    Gyorgy Hutvagner
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts, USA
    PLoS Biol 2:E98. 2004
    ....
  16. ncbi request reprint Evidence that siRNAs function as guides, not primers, in the Drosophila and human RNAi pathways
    Dianne S Schwarz
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Mol Cell 10:537-48. 2002
    ..We conclude that the underlying mechanism of RNAi is conserved between flies and mammals and that RNA-dependent RNA polymerases are not required for RNAi in these organisms...
  17. ncbi request reprint A protein sensor for siRNA asymmetry
    Yukihide Tomari
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Science 306:1377-80. 2004
    ..Thus, R2D2 is both a protein sensor for siRNA thermodynamic asymmetry and a licensing factor for entry of authentic siRNAs into the RNAi pathway...
  18. pmc Sorting of Drosophila small silencing RNAs partitions microRNA* strands into the RNA interference pathway
    Megha Ghildiyal
    Howard Hughes Medical Institute, Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    RNA 16:43-56. 2010
    ..Our findings provide the first genome-wide test for the idea that Drosophila small RNAs are sorted between Ago1 and Ago2 according to their duplex structure and the identity of their first nucleotide...
  19. pmc Endogenous siRNAs derived from transposons and mRNAs in Drosophila somatic cells
    Megha Ghildiyal
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Science 320:1077-81. 2008
    ..We propose that endo-siRNAs generated by the fly RNAi pathway silence selfish genetic elements in the soma, much as Piwi-interacting RNAs do in the germ line...
  20. ncbi request reprint Plant RNAi: How a viral silencing suppressor inactivates siRNA
    Phillip D Zamore
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    Curr Biol 14:R198-200. 2004
    ..The three-dimensional structure of an siRNA bound to the tombusvirus p19 protein--a suppressor of gene silencing--provides a first glimpse into how plant viruses can defeat their host's anti-viral RNAi defenses...
  21. pmc The Drosophila HP1 homolog Rhino is required for transposon silencing and piRNA production by dual-strand clusters
    Carla Klattenhoff
    University of Massachusetts Medical School, Worcester, MA 01605, USA
    Cell 138:1137-49. 2009
    ..Rhino thus appears to promote transcription of dual-strand clusters, leading to production of piRNAs that drive the ping-pong amplification cycle...
  22. ncbi request reprint RNAi: nature abhors a double-strand
    Gyorgy Hutvagner
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, Massachusetts 01655, USA
    Curr Opin Genet Dev 12:225-32. 2002
    ..RNA interference begins with the transformation of the double-stranded RNA into small RNAs that then guide a protein nuclease to destroy their mRNA targets...
  23. pmc Small silencing RNAs: an expanding universe
    Megha Ghildiyal
    Department of Biochemistry and Molecular Pharmacology and Howard Hughes Medical Institute, University of Massachusetts Medical School, 364 Plantation Street, Worcester, Massachusetts 01605, USA
    Nat Rev Genet 10:94-108. 2009
    ....
  24. pmc Sorting of Drosophila small silencing RNAs
    Yukihide Tomari
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Cell 130:299-308. 2007
    ..Thus, in flies small-RNA duplexes are actively sorted into Argonaute-containing complexes according to their intrinsic structures...
  25. ncbi request reprint Small silencing RNAs
    Christian Matranga
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 364 Plantation Street, Worcester, Massachusetts 01605 2324, USA
    Curr Biol 17:R789-93. 2007
  26. ncbi request reprint Why do miRNAs live in the miRNP?
    Dianne S Schwarz
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Genes Dev 16:1025-31. 2002
  27. ncbi request reprint Selective silencing by RNAi of a dominant allele that causes amyotrophic lateral sclerosis
    Hongliu Ding
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 364 Plantation St, Worcester, MA 01605 2324, USA
    Aging Cell 2:209-17. 2003
    ..Thus, RNAi is a promising therapy for ALS and other disorders caused by dominant, gain-of-function gene mutations...
  28. ncbi request reprint A distinct small RNA pathway silences selfish genetic elements in the germline
    Vasily V Vagin
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Science 313:320-4. 2006
    ..Our data suggest that rasiRNAs protect the fly germline through a silencing mechanism distinct from both the miRNA and RNA interference pathways...
  29. pmc Argonaute loading improves the 5' precision of both MicroRNAs and their miRNA* strands in flies
    Herve Seitz
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    Curr Biol 18:147-51. 2008
    ....
  30. pmc Design and delivery of antisense oligonucleotides to block microRNA function in cultured Drosophila and human cells
    Michael D Horwich
    Department of Biochemistry and Molecular Pharmacology and Howard Hughes Medical Institute, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    Nat Protoc 3:1537-49. 2008
    ..Our 3'-cholesterol-modified ASOs have enhanced potency, allowing miRNA inhibition for at least 7 d from a single transfection...
  31. pmc Five siRNAs targeting three SNPs may provide therapy for three-quarters of Huntington's disease patients
    Edith L Pfister
    Department of Medicine, Howard Hughes Medical Institute, University of Massachusetts Medical School, Worcester, MA 01655, USA
    Curr Biol 19:774-8. 2009
    ..We have designed and validated selective siRNAs for the three SNP sites, laying the foundation for allele-specific RNA interference (RNAi) therapy for HD...
  32. pmc Target RNA-directed trimming and tailing of small silencing RNAs
    Stefan L Ameres
    Howard Hughes Medical Institute and Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Science 328:1534-9. 2010
    ..Target complementarity also affects small RNA stability in human cells. These results provide an explanation for the partial complementarity between animal miRNAs and their targets...
  33. ncbi request reprint The Drosophila RNA methyltransferase, DmHen1, modifies germline piRNAs and single-stranded siRNAs in RISC
    Michael D Horwich
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Curr Biol 17:1265-72. 2007
    ..2'-O-methylation of siRNAs may be the final step in assembly of the RNAi-enzyme complex, RISC, occurring after an Argonaute-bound siRNA duplex is converted to single-stranded RNA...
  34. pmc Collapse of germline piRNAs in the absence of Argonaute3 reveals somatic piRNAs in flies
    Chengjian Li
    Department of Biochemistry and Molecular Pharmacology and Howard Hughes Medical Institute, University of Massachusetts Medical School, Worcester, 01605, USA
    Cell 137:509-21. 2009
    ..Transposons targeted by this second pathway often reside in the flamenco locus, which is expressed in somatic ovarian follicle cells, suggesting a role for piRNAs beyond the germline...
  35. ncbi request reprint MicroRNA biogenesis: drosha can't cut it without a partner
    Yukihide Tomari
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    Curr Biol 15:R61-4. 2005
    ....
  36. ncbi request reprint microPrimer: the biogenesis and function of microRNA
    Tingting Du
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Development 132:4645-52. 2005
    ..miRNAs function very much like siRNAs, but these two types of small RNAs can be distinguished by their distinct pathways for maturation and by the logic by which they regulate gene expression...
  37. pmc A single Argonaute protein mediates both transcriptional and posttranscriptional silencing in Schizosaccharomyces pombe
    Alla Sigova
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    Genes Dev 18:2359-67. 2004
    ..Our findings suggest that these three proteins fulfill a common biochemical function in distinct siRNA-directed silencing pathways...
  38. ncbi request reprint Rethinking the microprocessor
    Herve Seitz
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01532, USA
    Cell 125:827-9. 2006
    ..2006) report some of the structural features of the primary transcript that ensure that the Drosha-DGCR8 enzyme complex liberates precisely the correct precursor sequence, enabling production of a fully functional miRNA...
  39. pmc Designing siRNA that distinguish between genes that differ by a single nucleotide
    Dianne S Schwarz
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts, United States of America
    PLoS Genet 2:e140. 2006
    ..Our data suggest that siRNAs can be designed to discriminate between the wild-type and mutant alleles of many genes that differ by just a single nucleotide...
  40. ncbi request reprint Kinetic analysis of the RNAi enzyme complex
    Benjamin Haley
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    Nat Struct Mol Biol 11:599-606. 2004
    ..Finally, the position of the scissile phosphate on the target RNA seems to be determined during RISC assembly, before the siRNA encounters its RNA target...
  41. ncbi request reprint The RNA-induced silencing complex is a Mg2+-dependent endonuclease
    Dianne S Schwarz
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605 USA
    Curr Biol 14:787-91. 2004
    ..Thus, the RISC-component that mediates endonucleolytic cleavage of the target RNA remains to be identified...
  42. ncbi request reprint RISC assembly defects in the Drosophila RNAi mutant armitage
    Yukihide Tomari
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Cell 116:831-41. 2004
    ..Our results suggest that armi is required for RISC maturation...
  43. ncbi request reprint Perspective: machines for RNAi
    Yukihide Tomari
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    Genes Dev 19:517-29. 2005
    ..Here, we review our current understanding of how small RNAs are produced, how they are loaded into protein complexes, and how they repress gene expression...
  44. ncbi request reprint In vitro analysis of RNA interference in Drosophila melanogaster
    Benjamin Haley
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Lazare Research Building, Room 825, 364 Plantation Street, Worcester, MA 01605, USA
    Methods 30:330-6. 2003
    ..These lysates reproduce all of the known steps in the RNAi pathway in flies and mammals. Here we explain how to prepare and use Drosophila embryo lysates to dissect the mechanism of RNAi...
  45. ncbi request reprint Beginning to understand microRNA function
    Tingting Du
    University of Massachusetts Medical School, Worcester, MA 01605 2324, USA
    Cell Res 17:661-3. 2007
  46. ncbi request reprint siRNAs knock down hepatitis
    Phillip D Zamore
    Nat Med 9:266-7. 2003
  47. pmc Drosophila microRNAs are sorted into functionally distinct argonaute complexes after production by dicer-1
    Klaus Forstemann
    Gene Center and Munich Center for Integrated Protein Science CiPS M, Ludwig Maximilians Universitat Munchen, Feodor Lynen Str 25, D 81377 Munich, Germany
    Cell 130:287-97. 2007
    ..Thus, the two members of the Drosophila Ago subclade of Argonaute proteins are functionally specialized, but specific small RNA classes are not restricted to associate with Ago1 or Ago2...
  48. ncbi request reprint RNA silencing: genomic defence with a slice of pi
    Phillip D Zamore
    Nature 446:864-5. 2007
  49. ncbi request reprint Modular recognition of RNA by a human pumilio-homology domain
    Xiaoqiang Wang
    Laboratory of Structural Biology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Cell 110:501-12. 2002
    ..We have mutated these three side chains in one repeat, thereby altering the sequence specificity of Pumilio1. Thus, the high affinity and specificity of the PUM-HD for RNA is achieved using multiple copies of a simple repeated motif...
  50. pmc MicroRNA control of PHABULOSA in leaf development: importance of pairing to the microRNA 5' region
    Allison C Mallory
    Whitehead Institute for Biomedical Research, Cambridge, MA 02142 1479, USA
    EMBO J 23:3356-64. 2004
    ....

Research Grants23

  1. Understanding the Mechanism of RNA Interference (RNAi)
    Phillip Zamore; Fiscal Year: 2005
    ....
  2. Understanding microRNA Biogenesis and Function
    Phillip Zamore; Fiscal Year: 2007
    ....
  3. Understanding the Mechanism of RNA Interference (RNAi)
    Phillip Zamore; Fiscal Year: 2007
    ....
  4. Understanding microRNA Biogenesis and Function
    Phillip Zamore; Fiscal Year: 2007
    ..abstract_text> ..
  5. Understanding microRNA Biogenesis and Function
    Phillip Zamore; Fiscal Year: 2009
    ..abstract_text> ..
  6. Understanding the Mechanism of RNA Interference (RNAi)
    Phillip Zamore; Fiscal Year: 2009
    ....
  7. Biogenesis and function of the small temporal RNA let-7
    Phillip Zamore; Fiscal Year: 2006
    ..abstract_text> ..
  8. Biogenesis and function of the small temporal RNA let-7
    Phillip Zamore; Fiscal Year: 2003
    ..abstract_text> ..
  9. Understanding microRNA Biogenesis and Function
    Phillip D Zamore; Fiscal Year: 2010
    ..abstract_text> ..