Research Topics
Species | Phillip ZamoreSummaryAffiliation: University of Massachusetts Medical School Country: USA Publications
Research Grants
| Collaborators
|
Detail Information
Publications
Strand-specific libraries for high throughput RNA sequencing (RNA-Seq) prepared without poly(A) selectionZhao Zhang
Biochemistry and Molecular Pharmacology, and Howard Hughes Medical Institute, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA, 01605, USA
Silence 3:9. 2012..abstract:..- RNA: methods and protocols - a new seriesPhillip D Zamore
Howard Hughes Medical Institute and Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA, 01605, USA
Silence 3:7. 2012..ABSTRACT: This month, Silence launches a new series on methods and protocols to study silencing pathways and analyze nucleic acids and proteins... - Normal microRNA maturation and germ-line stem cell maintenance requires Loquacious, a double-stranded RNA-binding domain proteinKlaus Forstemann
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts, USA
PLoS Biol 3:e236. 2005..Thus, every known Drosophila RNase-III endonuclease is paired with a dsRBD protein that facilitates its function in small RNA biogenesis... - A 5'-uridine amplifies miRNA/miRNA* asymmetry in Drosophila by promoting RNA-induced silencing complex formationHerve Seitz
Laboratoire de Biologie Moleculaire Eucaryote, 118 route de Narbonne, Université Toulouse III Paul Sabatier UPS, F 31000 Toulouse, France
Silence 2:4. 2011..abstract:.. 
RNAi: double-stranded RNA directs the ATP-dependent cleavage of mRNA at 21 to 23 nucleotide intervalsP D Zamore
Department of Biochemistry and Molecular Biology, University of Massachusetts Medical School, Worcester 01655, USA
Cell 101:25-33. 2000..Cleavage occurs at sites 21-23 nucleotides apart, the same interval observed for the dsRNA itself, suggesting that the 21-23 nucleotide fragments from the dsRNA are guiding mRNA cleavage...- Ribo-gnome: the big world of small RNAsPhillip D Zamore
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
Science 309:1519-24. 2005.... - Ancient pathways programmed by small RNAsPhillip D Zamore
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Lazare Research Building, Room 825, 364 Plantation Street, Worcester, MA 01605, USA
Science 296:1265-9. 2002.... - RNA interference: listening to the sound of silenceP D Zamore
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, S6 240, 55 Lake Avenue North, Worcester, MA 01655, USA
Nat Struct Biol 8:746-50. 2001..The discovery of RNAi has changed our understanding of how cells guard their genomes, led to the development of new strategies for blocking gene function, and may yet yield RNA-based drugs to treat human disease... - RNA interference: big applause for silencing in StockholmPhillip D Zamore
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
Cell 127:1083-6. 2006..For this landmark discovery, Mello and Fire are honored with this year's Nobel Prize in Physiology or Medicine... - A microRNA in a multiple-turnover RNAi enzyme complexGyorgy Hutvagner
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Lazare Research Building, Room 825, 364 Plantation Street, Worcester, MA 01605, USA
Science 297:2056-60. 2002..Each let-7-containing complex directs multiple rounds of RNA cleavage, which explains the remarkable efficiency of the RNAi pathway in human cells... - A biochemical framework for RNA silencing in plantsGuiliang Tang
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
Genes Dev 17:49-63. 2003..Thus, endonuclease complexes guided by small RNAs are a common feature of RNA silencing in both animals and plants... - Passenger-strand cleavage facilitates assembly of siRNA into Ago2-containing RNAi enzyme complexesChristian Matranga
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
Cell 123:607-20. 2005..For siRNAs, virtually all RISC is assembled through this cleavage-assisted mechanism. In contrast, passenger-strand cleavage is not important for the incorporation of miRNAs that derive from mismatched duplexes... - Asymmetry in the assembly of the RNAi enzyme complexDianne S Schwarz
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Lazare Research Building, 364 Plantation Street, Worcester, MA 01605, USA
Cell 115:199-208. 2003..Thus, the common step of RISC assembly is an unexpected source of asymmetry for both siRNA function and miRNA biogenesis... - Biochemical dissection of RNA silencing in plantsGuiliang Tang
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA, USA
Methods Mol Biol 257:223-44. 2004..These comprehensive protocols should prove useful in the further dissection of the plant RNA silencing pathway, as well as for the validation of the predicted targets of endogenous plant microRNAs... - Sequence-specific inhibition of small RNA functionGyorgy Hutvagner
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts, USA
PLoS Biol 2:E98. 2004.... - Evidence that siRNAs function as guides, not primers, in the Drosophila and human RNAi pathwaysDianne S Schwarz
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
Mol Cell 10:537-48. 2002..We conclude that the underlying mechanism of RNAi is conserved between flies and mammals and that RNA-dependent RNA polymerases are not required for RNAi in these organisms... - A protein sensor for siRNA asymmetryYukihide Tomari
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
Science 306:1377-80. 2004..Thus, R2D2 is both a protein sensor for siRNA thermodynamic asymmetry and a licensing factor for entry of authentic siRNAs into the RNAi pathway... - Sorting of Drosophila small silencing RNAs partitions microRNA* strands into the RNA interference pathwayMegha Ghildiyal
Howard Hughes Medical Institute, Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
RNA 16:43-56. 2010..Our findings provide the first genome-wide test for the idea that Drosophila small RNAs are sorted between Ago1 and Ago2 according to their duplex structure and the identity of their first nucleotide... - Endogenous siRNAs derived from transposons and mRNAs in Drosophila somatic cellsMegha Ghildiyal
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
Science 320:1077-81. 2008..We propose that endo-siRNAs generated by the fly RNAi pathway silence selfish genetic elements in the soma, much as Piwi-interacting RNAs do in the germ line... - Plant RNAi: How a viral silencing suppressor inactivates siRNAPhillip D Zamore
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
Curr Biol 14:R198-200. 2004..The three-dimensional structure of an siRNA bound to the tombusvirus p19 protein--a suppressor of gene silencing--provides a first glimpse into how plant viruses can defeat their host's anti-viral RNAi defenses... - The Drosophila HP1 homolog Rhino is required for transposon silencing and piRNA production by dual-strand clustersCarla Klattenhoff
University of Massachusetts Medical School, Worcester, MA 01605, USA
Cell 138:1137-49. 2009..Rhino thus appears to promote transcription of dual-strand clusters, leading to production of piRNAs that drive the ping-pong amplification cycle... - RNAi: nature abhors a double-strandGyorgy Hutvagner
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, Massachusetts 01655, USA
Curr Opin Genet Dev 12:225-32. 2002..RNA interference begins with the transformation of the double-stranded RNA into small RNAs that then guide a protein nuclease to destroy their mRNA targets... - Small silencing RNAs: an expanding universeMegha Ghildiyal
Department of Biochemistry and Molecular Pharmacology and Howard Hughes Medical Institute, University of Massachusetts Medical School, 364 Plantation Street, Worcester, Massachusetts 01605, USA
Nat Rev Genet 10:94-108. 2009.... - Sorting of Drosophila small silencing RNAsYukihide Tomari
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
Cell 130:299-308. 2007..Thus, in flies small-RNA duplexes are actively sorted into Argonaute-containing complexes according to their intrinsic structures... - Small silencing RNAsChristian Matranga
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 364 Plantation Street, Worcester, Massachusetts 01605-2324, USA
Curr Biol 17:R789-93. 2007 - Why do miRNAs live in the miRNP?Dianne S Schwarz
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
Genes Dev 16:1025-31. 2002 - Selective silencing by RNAi of a dominant allele that causes amyotrophic lateral sclerosisHongliu Ding
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 364 Plantation St, Worcester, MA 01605-2324, USA
Aging Cell 2:209-17. 2003..Thus, RNAi is a promising therapy for ALS and other disorders caused by dominant, gain-of-function gene mutations... - A distinct small RNA pathway silences selfish genetic elements in the germlineVasily V Vagin
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
Science 313:320-4. 2006..Our data suggest that rasiRNAs protect the fly germline through a silencing mechanism distinct from both the miRNA and RNA interference pathways... - Argonaute loading improves the 5' precision of both MicroRNAs and their miRNA* strands in fliesHerve Seitz
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
Curr Biol 18:147-51. 2008.... - Design and delivery of antisense oligonucleotides to block microRNA function in cultured Drosophila and human cellsMichael D Horwich
Department of Biochemistry and Molecular Pharmacology and Howard Hughes Medical Institute, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
Nat Protoc 3:1537-49. 2008..Our 3'-cholesterol-modified ASOs have enhanced potency, allowing miRNA inhibition for at least 7 d from a single transfection... - Five siRNAs targeting three SNPs may provide therapy for three-quarters of Huntington's disease patientsEdith L Pfister
Department of Medicine, Howard Hughes Medical Institute, University of Massachusetts Medical School, Worcester, MA 01655, USA
Curr Biol 19:774-8. 2009..We have designed and validated selective siRNAs for the three SNP sites, laying the foundation for allele-specific RNA interference (RNAi) therapy for HD... - Target RNA-directed trimming and tailing of small silencing RNAsStefan L Ameres
Howard Hughes Medical Institute and Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
Science 328:1534-9. 2010..Target complementarity also affects small RNA stability in human cells. These results provide an explanation for the partial complementarity between animal miRNAs and their targets... - The Drosophila RNA methyltransferase, DmHen1, modifies germline piRNAs and single-stranded siRNAs in RISCMichael D Horwich
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
Curr Biol 17:1265-72. 2007..2'-O-methylation of siRNAs may be the final step in assembly of the RNAi-enzyme complex, RISC, occurring after an Argonaute-bound siRNA duplex is converted to single-stranded RNA... - Collapse of germline piRNAs in the absence of Argonaute3 reveals somatic piRNAs in fliesChengjian Li
Department of Biochemistry and Molecular Pharmacology and Howard Hughes Medical Institute, University of Massachusetts Medical School, Worcester, 01605, USA
Cell 137:509-21. 2009..Transposons targeted by this second pathway often reside in the flamenco locus, which is expressed in somatic ovarian follicle cells, suggesting a role for piRNAs beyond the germline... - MicroRNA biogenesis: drosha can't cut it without a partnerYukihide Tomari
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
Curr Biol 15:R61-4. 2005.... - microPrimer: the biogenesis and function of microRNATingting Du
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
Development 132:4645-52. 2005..miRNAs function very much like siRNAs, but these two types of small RNAs can be distinguished by their distinct pathways for maturation and by the logic by which they regulate gene expression... - A single Argonaute protein mediates both transcriptional and posttranscriptional silencing in Schizosaccharomyces pombeAlla Sigova
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
Genes Dev 18:2359-67. 2004..Our findings suggest that these three proteins fulfill a common biochemical function in distinct siRNA-directed silencing pathways... - Rethinking the microprocessorHerve Seitz
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01532, USA
Cell 125:827-9. 2006..2006) report some of the structural features of the primary transcript that ensure that the Drosha-DGCR8 enzyme complex liberates precisely the correct precursor sequence, enabling production of a fully functional miRNA... - Designing siRNA that distinguish between genes that differ by a single nucleotideDianne S Schwarz
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts, United States of America
PLoS Genet 2:e140. 2006..Our data suggest that siRNAs can be designed to discriminate between the wild-type and mutant alleles of many genes that differ by just a single nucleotide... - Kinetic analysis of the RNAi enzyme complexBenjamin Haley
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
Nat Struct Mol Biol 11:599-606. 2004..Finally, the position of the scissile phosphate on the target RNA seems to be determined during RISC assembly, before the siRNA encounters its RNA target... - The RNA-induced silencing complex is a Mg2+-dependent endonucleaseDianne S Schwarz
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605 USA
Curr Biol 14:787-91. 2004..Thus, the RISC-component that mediates endonucleolytic cleavage of the target RNA remains to be identified... - RISC assembly defects in the Drosophila RNAi mutant armitageYukihide Tomari
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
Cell 116:831-41. 2004..Our results suggest that armi is required for RISC maturation... - Perspective: machines for RNAiYukihide Tomari
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
Genes Dev 19:517-29. 2005..Here, we review our current understanding of how small RNAs are produced, how they are loaded into protein complexes, and how they repress gene expression... - In vitro analysis of RNA interference in Drosophila melanogasterBenjamin Haley
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Lazare Research Building, Room 825, 364 Plantation Street, Worcester, MA 01605, USA
Methods 30:330-6. 2003..These lysates reproduce all of the known steps in the RNAi pathway in flies and mammals. Here we explain how to prepare and use Drosophila embryo lysates to dissect the mechanism of RNAi... - Beginning to understand microRNA functionTingting Du
University of Massachusetts Medical School, Worcester, MA 01605-2324, USA
Cell Res 17:661-3. 2007 - siRNAs knock down hepatitisPhillip D Zamore
Nat Med 9:266-7. 2003 - Drosophila microRNAs are sorted into functionally distinct argonaute complexes after production by dicer-1Klaus Forstemann
Gene Center and Munich Center for Integrated Protein Science CiPS M, Ludwig Maximilians Universitat Munchen, Feodor Lynen Str 25, D 81377 Munich, Germany
Cell 130:287-97. 2007..Thus, the two members of the Drosophila Ago subclade of Argonaute proteins are functionally specialized, but specific small RNA classes are not restricted to associate with Ago1 or Ago2... - RNA silencing: genomic defence with a slice of piPhillip D Zamore
Nature 446:864-5. 2007 - Modular recognition of RNA by a human pumilio-homology domainXiaoqiang Wang
Laboratory of Structural Biology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
Cell 110:501-12. 2002..We have mutated these three side chains in one repeat, thereby altering the sequence specificity of Pumilio1. Thus, the high affinity and specificity of the PUM-HD for RNA is achieved using multiple copies of a simple repeated motif... - MicroRNA control of PHABULOSA in leaf development: importance of pairing to the microRNA 5' regionAllison C Mallory
Whitehead Institute for Biomedical Research, Cambridge, MA 02142 1479, USA
EMBO J 23:3356-64. 2004....
Research Grants
- Understanding the Mechanism of RNA Interference (RNAi)Phillip Zamore; Fiscal Year: 2005....
- Understanding microRNA Biogenesis and FunctionPhillip Zamore; Fiscal Year: 2007....
- Understanding the Mechanism of RNA Interference (RNAi)Phillip Zamore; Fiscal Year: 2007....
- Understanding microRNA Biogenesis and FunctionPhillip Zamore; Fiscal Year: 2007..abstract_text> ..
- Understanding microRNA Biogenesis and FunctionPhillip Zamore; Fiscal Year: 2009..abstract_text> ..
- Understanding the Mechanism of RNA Interference (RNAi)Phillip Zamore; Fiscal Year: 2009....
- Biogenesis and function of the small temporal RNA let-7Phillip Zamore; Fiscal Year: 2006..abstract_text> ..
- Biogenesis and function of the small temporal RNA let-7Phillip Zamore; Fiscal Year: 2003..abstract_text> ..
- Understanding microRNA Biogenesis and FunctionPhillip D Zamore; Fiscal Year: 2010..abstract_text> ..