Hongtao Yu

Summary

Affiliation: University of Texas Southwestern Medical Center
Country: USA

Publications

  1. pmc Phosphorylation-enabled binding of SGO1-PP2A to cohesin protects sororin and centromeric cohesion during mitosis
    Hong Liu
    Department of Pharmacology, Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, 6001 Forest Park Road, Dallas, Texas 75390, USA
    Nat Cell Biol 15:40-9. 2013
  2. pmc ZNF198 stabilizes the LSD1-CoREST-HDAC1 complex on chromatin through its MYM-type zinc fingers
    Christian B Gocke
    Howard Hughes Medical Institute, Department of Pharmacology, The University of Texas Southwestern Medical Center, Dallas, Texas, USA
    PLoS ONE 3:e3255. 2008
  3. pmc Multiple anaphase-promoting complex/cyclosome degrons mediate the degradation of human Sgo1
    Zemfira Karamysheva
    Department of Pharmacology, Howard Hughes Medical Institute, University of Texas Southwestern Medical Center at Dallas, Dallas, Texas, 75390, USA
    J Biol Chem 284:1772-80. 2009
  4. doi request reprint Chromosome biology: Wapl spreads its wings
    Hongtao Yu
    Department of Pharmacology, Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, 6001 Forest Park Road, Dallas, TX 75390, USA Electronic address
    Curr Biol 23:R923-5. 2013
  5. pmc The Smc complexes in DNA damage response
    Nan Wu
    Department of Pharmacology, Howard Hughes Medical Institute, 6001 Forest Park Road, Dallas, TX 75390, USA
    Cell Biosci 2:5. 2012
  6. pmc Tango between ubiquitin ligase and deubiquitinase keeps cyclin A tag free
    Hongtao Yu
    Department of Pharmacology, University of Texas Southwestern Medical Center, 6001 Forest Park Road, Dallas, TX 75390, USA
    Mol Cell 42:409-10. 2011
  7. pmc Structural activation of Mad2 in the mitotic spindle checkpoint: the two-state Mad2 model versus the Mad2 template model
    Hongtao Yu
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, 75390, USA
    J Cell Biol 173:153-7. 2006
  8. ncbi request reprint Chk1: a double agent in cell cycle checkpoints
    Hongtao Yu
    Department of Pharmacology, University of Texas Southwestern Medical Center, 6001 Forest Park Road, Dallas, TX 75390, USA
    Dev Cell 12:167-8. 2007
  9. ncbi request reprint Cdc20: a WD40 activator for a cell cycle degradation machine
    Hongtao Yu
    Department of Pharmacology, The University of Texas Southwestern Medical Center, 6001 Forest Park Road, Dallas, TX 75390 9041, USA
    Mol Cell 27:3-16. 2007
  10. pmc PP2A as a mercenary for warring kinases in the egg
    Hongtao Yu
    Department of Pharmacology, University of Texas Southwestern Medical Center, 6001 Forest Park Road, Dallas, TX 75390 9041, USA
    Proc Natl Acad Sci U S A 104:17245-6. 2007

Collaborators

Detail Information

Publications69

  1. pmc Phosphorylation-enabled binding of SGO1-PP2A to cohesin protects sororin and centromeric cohesion during mitosis
    Hong Liu
    Department of Pharmacology, Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, 6001 Forest Park Road, Dallas, Texas 75390, USA
    Nat Cell Biol 15:40-9. 2013
    ..PP2A-orchestrated, site-selective dephosphorylation of cohesin and its regulators underlies centromeric cohesion protection...
  2. pmc ZNF198 stabilizes the LSD1-CoREST-HDAC1 complex on chromatin through its MYM-type zinc fingers
    Christian B Gocke
    Howard Hughes Medical Institute, Department of Pharmacology, The University of Texas Southwestern Medical Center, Dallas, Texas, USA
    PLoS ONE 3:e3255. 2008
    ....
  3. pmc Multiple anaphase-promoting complex/cyclosome degrons mediate the degradation of human Sgo1
    Zemfira Karamysheva
    Department of Pharmacology, Howard Hughes Medical Institute, University of Texas Southwestern Medical Center at Dallas, Dallas, Texas, 75390, USA
    J Biol Chem 284:1772-80. 2009
    ..Finally, we show that the spindle checkpoint kinase Bub1 contributes to the maintenance of Sgo1 steady-state protein levels in an APC/C-independent mechanism...
  4. doi request reprint Chromosome biology: Wapl spreads its wings
    Hongtao Yu
    Department of Pharmacology, Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, 6001 Forest Park Road, Dallas, TX 75390, USA Electronic address
    Curr Biol 23:R923-5. 2013
    ..Two new studies reveal that, among other functions, the wings apart-like protein (Wapl) coordinates cohesin removal with decatenation of sister chromosomes during mitosis in mammalian cells. ..
  5. pmc The Smc complexes in DNA damage response
    Nan Wu
    Department of Pharmacology, Howard Hughes Medical Institute, 6001 Forest Park Road, Dallas, TX 75390, USA
    Cell Biosci 2:5. 2012
    ....
  6. pmc Tango between ubiquitin ligase and deubiquitinase keeps cyclin A tag free
    Hongtao Yu
    Department of Pharmacology, University of Texas Southwestern Medical Center, 6001 Forest Park Road, Dallas, TX 75390, USA
    Mol Cell 42:409-10. 2011
    ..In this issue of Molecular Cell, Huang et al. (2011) provide a counterintuitive example of a USP residing in an E3 complex, and establish Usp37 as a gatekeeper of APC/C-mediated ubiquitination of cyclin A...
  7. pmc Structural activation of Mad2 in the mitotic spindle checkpoint: the two-state Mad2 model versus the Mad2 template model
    Hongtao Yu
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, 75390, USA
    J Cell Biol 173:153-7. 2006
    ..I review the recent structural, biochemical, and cell biological data on Mad2, discuss the differences between the two models, and propose experiments that test their key principles...
  8. ncbi request reprint Chk1: a double agent in cell cycle checkpoints
    Hongtao Yu
    Department of Pharmacology, University of Texas Southwestern Medical Center, 6001 Forest Park Road, Dallas, TX 75390, USA
    Dev Cell 12:167-8. 2007
    ..In this issue of Developmental Cell, Zachos et al.(2007) present evidence to indicate that Chk1 also plays a critical role in the spindle checkpoint, suggesting an interplay between the DNA damage and spindle checkpoints...
  9. ncbi request reprint Cdc20: a WD40 activator for a cell cycle degradation machine
    Hongtao Yu
    Department of Pharmacology, The University of Texas Southwestern Medical Center, 6001 Forest Park Road, Dallas, TX 75390 9041, USA
    Mol Cell 27:3-16. 2007
    ..This review summarizes recent progress toward the understanding of the functions of Cdc20, the mechanisms by which it activates APC/C, and its regulation by phosphorylation and by association with its binding proteins...
  10. pmc PP2A as a mercenary for warring kinases in the egg
    Hongtao Yu
    Department of Pharmacology, University of Texas Southwestern Medical Center, 6001 Forest Park Road, Dallas, TX 75390 9041, USA
    Proc Natl Acad Sci U S A 104:17245-6. 2007
  11. ncbi request reprint Regulation of APC-Cdc20 by the spindle checkpoint
    Hongtao Yu
    Department of Pharmacology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390 9041, USA
    Curr Opin Cell Biol 14:706-14. 2002
    ..Therefore, the checkpoint proteins form a complex intracellular signalling network to inhibit the anaphase-promoting complex...
  12. ncbi request reprint Bub1 multitasking in mitosis
    Hongtao Yu
    Department of Pharmacology, The University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    Cell Cycle 4:262-5. 2005
    ..Therefore, Bub1 performs multiple tasks in mitosis that ensure the proper inheritance of chromosomes...
  13. pmc Phosphorylation- and polo-box-dependent binding of Plk1 to Bub1 is required for the kinetochore localization of Plk1
    Wei Qi
    Department of Pharmacology, The University of Texas Southwestern Medical Center, Dallas, TX 75390 9041, USA
    Mol Biol Cell 17:3705-16. 2006
    ..Our results suggest that phosphorylation of Bub1 at T609 by Cdk1 creates a docking site for the PBD of Plk1 and facilitates the kinetochore recruitment of Plk1...
  14. pmc p31comet blocks Mad2 activation through structural mimicry
    Maojun Yang
    Department of Pharmacology, The University of Texas Southwestern Medical Center, 6001 Forest Park Road, Dallas, TX 75390, USA
    Cell 131:744-55. 2007
    ..p31(comet) adopts a fold strikingly similar to that of Mad2 and binds at the dimerization interface of Mad2. Thus, p31(comet) exploits the two-state behavior of Mad2 to block its activation by acting as an "anti-Mad2."..
  15. pmc Insights into mad2 regulation in the spindle checkpoint revealed by the crystal structure of the symmetric mad2 dimer
    Maojun Yang
    Department of Pharmacology, The University of Texas Southwestern Medical Center, Dallas, Texas, United States of America
    PLoS Biol 6:e50. 2008
    ..Collectively, our results establish the existence of a symmetric Mad2 dimer and provide insights into Mad1-assisted conformational activation of Mad2 in the spindle checkpoint...
  16. pmc Human MMS21/NSE2 is a SUMO ligase required for DNA repair
    Patrick Ryan Potts
    Department of Pharmacology, The University of Texas Southwestern Medical Center, Dallas, 75390 9041, USA
    Mol Cell Biol 25:7021-32. 2005
    ..Our findings suggest that the human SMC5/6 complex and the SUMO ligase activity of hMMS21 are required for the prevention of DNA damage-induced apoptosis by facilitating DNA repair in human cells...
  17. pmc Phosphorylation of the spindle checkpoint protein Mad2 regulates its conformational transition
    Soonjoung Kim
    Department of Pharmacology, Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, 6001 Forest Park Road, Dallas, TX 75390 9041, USA
    Proc Natl Acad Sci U S A 107:19772-7. 2010
    ..Our results indicate that Mad2 phosphorylation inhibits its function through differentially regulating its binding to Mad1 and Cdc20 and establish that the conformational change of Mad2 is regulated by posttranslational mechanisms...
  18. pmc Human Bub1 protects centromeric sister-chromatid cohesion through Shugoshin during mitosis
    Zhanyun Tang
    Department of Pharmacology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390 9041, USA
    Proc Natl Acad Sci U S A 101:18012-7. 2004
    ..Bub1 maintains the steady-state levels and centromeric localization of Sgo1. Therefore, Bub1 protects centromeric cohesion through Shugoshin in mitosis...
  19. pmc Mitotic centromeric targeting of HP1 and its binding to Sgo1 are dispensable for sister-chromatid cohesion in human cells
    Jungseog Kang
    Department of Pharmacology, Howard Hughes Medical Institute, USA
    Mol Biol Cell 22:1181-90. 2011
    ....
  20. pmc Conformation-specific binding of p31(comet) antagonizes the function of Mad2 in the spindle checkpoint
    Guohong Xia
    Department of Pharmacology, The University of Texas, Southwestern Medical Center at Dallas, Dallas, TX 75390, USA
    EMBO J 23:3133-43. 2004
    ..Therefore, our results suggest that p31(comet) counteracts the function of Mad2 and is required for the silencing of the spindle checkpoint...
  21. ncbi request reprint Structural basis for CoREST-dependent demethylation of nucleosomes by the human LSD1 histone demethylase
    Maojun Yang
    Department of Pharmacology, The University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    Mol Cell 23:377-87. 2006
    ..This spatially separated, multivalent nucleosome binding mode may apply to other chromatin-modifying enzymes that generally contain multiple nucleosome binding modules...
  22. ncbi request reprint Phospho-H2A and cohesin specify distinct tension-regulated Sgo1 pools at kinetochores and inner centromeres
    Hong Liu
    Howard Hughes Medical Institute, Department of Pharmacology, University of Texas Southwestern Medical Center, 6001 Forest Park Road, Dallas, TX 75390, USA
    Curr Biol 23:1927-33. 2013
    ..Our study suggests that Bub1-mediated H2A phosphorylation penetrates kinetochores and that this histone mark contributes to a tension-sensitive Sgo1-based molecular switch for chromosome segregation...
  23. ncbi request reprint Phosphorylation of Cdc20 by Bub1 provides a catalytic mechanism for APC/C inhibition by the spindle checkpoint
    Zhanyun Tang
    Department of Pharmacology, The University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Boulevard, Dallas, TX 75390, USA
    Mol Cell 16:387-97. 2004
    ..We speculate that inhibition of APC/C(Cdc20) by Bub1 in a catalytic fashion may partly account for the exquisite sensitivity of the spindle checkpoint...
  24. pmc Defining pathways of spindle checkpoint silencing: functional redundancy between Cdc20 ubiquitination and p31(comet)
    Luying Jia
    Howard Hughes Medical Institute and Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    Mol Biol Cell 22:4227-35. 2011
    ..Thus both p31(comet) and ubiquitination of Cdc20 are critical mechanisms of checkpoint inactivation. They act redundantly to promote Mad2 dissociation from Cdc20...
  25. ncbi request reprint PP2A is required for centromeric localization of Sgo1 and proper chromosome segregation
    Zhanyun Tang
    Department of Pharmacology, The University of Texas Southwestern Medical Center, 6001 Forest Park Road, Dallas, Texas 75390, USA
    Dev Cell 10:575-85. 2006
    ..Our findings suggest that Bub1 targets PP2A to centromeres, which in turn maintains Sgo1 at centromeres by counteracting Plk1-mediated chromosome removal of Sgo1...
  26. pmc Structure and substrate recruitment of the human spindle checkpoint kinase Bub1
    Jungseog Kang
    Howard Hughes Medical Institute, The University of Texas Southwestern Medical Center, 6001 Forest Park Road, Dallas, TX 75390, USA
    Mol Cell 32:394-405. 2008
    ..The KEN boxes of Bub1 are required for the spindle checkpoint in human cells. Therefore, its unusual active-site conformation and mode of substrate recruitment suggest that Bub1 has an exquisitely tuned specificity for Cdc20...
  27. pmc Structural analysis of human Cdc20 supports multisite degron recognition by APC/C
    Wei Tian
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    Proc Natl Acad Sci U S A 109:18419-24. 2012
    ..We propose that low-cooperativity, multisite target recognition enables APC/C to robustly ubiquitinate diverse substrates and helps to drive cell cycle oscillations...
  28. pmc Scc1 sumoylation by Mms21 promotes sister chromatid recombination through counteracting Wapl
    Nan Wu
    Howard Hughes Medical Institute, Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    Genes Dev 26:1473-85. 2012
    ..We propose that Scc1 sumoylation by Mms21 promotes SCR by antagonizing Wapl at a step after cohesin loading at DSBs and in a way not solely dependent on Smc3 acetylation...
  29. pmc Kinase signaling in the spindle checkpoint
    Jungseog Kang
    Department of Pharmacology, Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    J Biol Chem 284:15359-63. 2009
    ..Increasing evidence also indicates that the checkpoint kinases not only help to generate the wait anaphase signal but also actively correct kinetochore-microtubule attachment defects...
  30. ncbi request reprint The Mad2 spindle checkpoint protein has two distinct natively folded states
    Xuelian Luo
    Department of Pharmacology, The University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, Texas 75390, USA
    Nat Struct Mol Biol 11:338-45. 2004
    ..Our results suggest that the unusual two-state behavior of Mad2 is critical for spindle checkpoint signaling...
  31. pmc PICH and BLM limit histone association with anaphase centromeric DNA threads and promote their resolution
    Yuwen Ke
    Deparment of Pharmacology, Howard Hughes Medical Institute, Dallas, TX, USA
    EMBO J 30:3309-21. 2011
    ....
  32. pmc Phosphorylation-facilitated sumoylation of MEF2C negatively regulates its transcriptional activity
    Jungseog Kang
    Department of Pharmacology, The University of Texas Southwestern Medical Center, Dallas, TX 75390 9041, USA
    BMC Biochem 7:5. 2006
    ..The myocyte enhancer factor 2 (MEF2) family of transcription factors plays an important role in regulating gene expression during myogenesis and has been recently shown to be sumoylated...
  33. ncbi request reprint KEN-box-dependent degradation of the Bub1 spindle checkpoint kinase by the anaphase-promoting complex/cyclosome
    Wei Qi
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9041, USA
    J Biol Chem 282:3672-9. 2007
    ..Nevertheless, our study clearly demonstrates that Bub1, an APC/C inhibitor, is also an APC/C substrate. The antagonistic relationship between Bub1 and APC/C may help to prevent the premature accumulation of Bub1 during G1...
  34. pmc Human SMC5/6 complex promotes sister chromatid homologous recombination by recruiting the SMC1/3 cohesin complex to double-strand breaks
    Patrick Ryan Potts
    Department of Pharmacology, The University of Texas Southwestern Medical Center, Dallas, TX 75390 9041, USA
    EMBO J 25:3377-88. 2006
    ..Our results establish a mechanism by which the hSMC5/6 complex promotes DNA repair and suggest a novel strategy to improve the efficiency of gene targeting in mammalian somatic cells...
  35. pmc Mechanistic insight into the allosteric activation of a ubiquitin-conjugating enzyme by RING-type ubiquitin ligases
    Engin Ozkan
    Department of Biochemistry and Pharmacology and Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, 6001 Forest Park Road, Dallas, TX 75390, USA
    Proc Natl Acad Sci U S A 102:18890-5. 2005
    ..Our studies reveal structural determinants for communication between distal functional sites of E2s and suggest that RING-type E3s activate E2s allosterically...
  36. pmc Structure of human Mad1 C-terminal domain reveals its involvement in kinetochore targeting
    Soonjoung Kim
    Department of Pharmacology, University of Texas Southwestern Medical Center, 6001 Forest Park Road, Dallas, TX 75390, USA
    Proc Natl Acad Sci U S A 109:6549-54. 2012
    ..Our results indicate that CTD is a part of an extensive kinetochore-binding interface of Mad1, and rationalize graded kinetochore targeting of Mad1 during checkpoint signaling...
  37. ncbi request reprint Purification and assay of Mad2: a two-state inhibitor of anaphase-promoting complex/cyclosome
    Xuelian Luo
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, TX 75390 9041, USA
    Methods Enzymol 398:246-55. 2005
    ..This article describes methods for the purification of the two Mad2 conformers and for the analysis of their activities in APC/C inhibition in Xenopus egg extracts...
  38. ncbi request reprint Identification of two novel components of the human NDC80 kinetochore complex
    Rajnish Bharadwaj
    Department of Pharmacology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390, USA
    J Biol Chem 279:13076-85. 2004
    ..Thus, hSPC25 is an essential kinetochore component that plays a significant role in proper execution of mitotic events...
  39. ncbi request reprint Systematic identification and analysis of mammalian small ubiquitin-like modifier substrates
    Christian B Gocke
    Department of Pharmacology, The University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    J Biol Chem 280:5004-12. 2005
    ..Therefore, sumoylation appears to regulate the functions of its substrates through multiple, context-dependent mechanisms...
  40. ncbi request reprint The SMC5/6 complex maintains telomere length in ALT cancer cells through SUMOylation of telomere-binding proteins
    Patrick Ryan Potts
    Department of Pharmacology, The University of Texas Southwestern Medical Center, 6001 Forest Park Road, Dallas, Texas 75390 9041, USA
    Nat Struct Mol Biol 14:581-90. 2007
    ..Thus, the SMC5/6 complex facilitates telomere HR and elongation in ALT cells by promoting APB formation through SUMOylation of telomere-binding proteins...
  41. pmc Autophosphorylation-dependent activation of human Mps1 is required for the spindle checkpoint
    Jungseog Kang
    Department of Pharmacology, University of Texas Southwestern Medical Center, 6001 Forest Park Road, Dallas, TX 75390 9041, USA
    Proc Natl Acad Sci U S A 104:20232-7. 2007
    ..We speculate that the kinetochore localization of Mps1 raises its local concentration, leading to its activation during mitosis through more efficient trans autophosphorylation...
  42. ncbi request reprint Structural basis of histone demethylation by LSD1 revealed by suicide inactivation
    Maojun Yang
    Department of Pharmacology, The University of Texas Southwestern Medical Center, 6001 Forest Park Road, Dallas, Texas 75390, USA
    Nat Struct Mol Biol 14:535-9. 2007
    ..The unusual backbone conformation of LSD1-bound H3 suggests a strategy for designing potent LSD1 inhibitors with therapeutic potential...
  43. ncbi request reprint Structural basis for the inhibition of the LSD1 histone demethylase by the antidepressant trans-2-phenylcyclopropylamine
    Maojun Yang
    Departments of Pharmacology, The University of Texas Southwestern Medical Center, 6001 Forest Park Road, Dallas, Texas 75390, USA
    Biochemistry 46:8058-65. 2007
    ..This study thus provides the basis for designing more potent inhibitors of LSD1 that contain substitutions on the phenyl ring of PCPA to fully engage neighboring residues...
  44. pmc Structure of the human cohesin inhibitor Wapl
    Zhuqing Ouyang
    Howard Hughes Medical Institute, Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    Proc Natl Acad Sci U S A 110:11355-60. 2013
    ..Thus, Wapl-N forms extensive interactions with Pds5 and Scc1-SA2. Wapl-C interacts with other cohesin subunits and possibly unknown effectors to trigger cohesin release from chromatin. ..
  45. pmc Protein metamorphosis: the two-state behavior of Mad2
    Xuelian Luo
    Department of Pharmacology, University of Texas Southwestern Medical Center, 6001 Forest Park Road, Dallas, TX 75390, USA
    Structure 16:1616-25. 2008
    ..This review summarizes recent structural and biochemical studies on the two-state behavior of Mad2 and discusses the generality and implications of structural malleability of proteins...
  46. ncbi request reprint Crm1-mediated nuclear export of Cdc14 is required for the completion of cytokinesis in budding yeast
    Joshua Bembenek
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    Cell Cycle 4:961-71. 2005
    ..Our results suggest a requirement for Crm1p-dependent nuclear export of Cdc14p in coordinating mitotic exit and cytokinesis in budding yeast...
  47. pmc Mutual regulation between the spindle checkpoint and APC/C
    Soonjoung Kim
    Howard Hughes Medical Institute, Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, TX 75390, United States
    Semin Cell Dev Biol 22:551-8. 2011
    ....
  48. ncbi request reprint The spindle checkpoint, aneuploidy, and cancer
    Rajnish Bharadwaj
    Department of Pharmacology, UT Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, TX 75390 9041, USA
    Oncogene 23:2016-27. 2004
    ..We review recent progress toward the understanding of the molecular mechanism of the spindle checkpoint and its role in guarding genome integrity at the chromosome level...
  49. ncbi request reprint The Mad2 spindle checkpoint protein undergoes similar major conformational changes upon binding to either Mad1 or Cdc20
    Xuelian Luo
    Department of Biochemistry, 5323 Harry Hines Boulevard, Dallas, TX 75390, USA
    Mol Cell 9:59-71. 2002
    ..Our data suggest that, upon checkpoint activation, Mad1 recruits Mad2 to unattached kinetochores and may promote binding of Mad2 to Cdc20...
  50. doi request reprint Identification of SUMO targets through in vitro expression cloning
    Christian B Gocke
    Department of Pharmacology, The University of Texas Southwestern Medical Center, Dallas, TX, USA
    Methods Mol Biol 497:51-61. 2009
    ..IVEC allows for immediate validation and analysis of substrates through in vitro reconstitution. Furthermore, this method can be easily adapted to identify substrates of specific SUMO ligases...
  51. ncbi request reprint Functional analysis of the spindle-checkpoint proteins using an in vitro ubiquitination assay
    Zhanyun Tang
    Department of Pharmacology, UT Southwestern Medical Center, Dallas, USA
    Methods Mol Biol 281:227-42. 2004
    ..This assay is extremely useful in dissecting the biochemical functions of various spindle-checkpoint proteins...
  52. doi request reprint A protective chaperone for the kinetochore adaptor Bub3
    Zhejian Ji
    Howard Hughes Medical Institute, Department of Pharmacology, University of Texas Southwestern Medical Center, 6001 Forest Park Road, Dallas, TX 75390, USA
    Dev Cell 28:223-4. 2014
    ..2014) and Toledo et al. (2014) identify BuGZ as an interacting protein of the kinetochore adaptor Bub3 and show that it promotes the stabilization and kinetochore loading of Bub3, chromosome alignment, and mitotic progression. ..
  53. doi request reprint Mitosis: short-circuiting spindle checkpoint signaling
    Xuelian Luo
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    Curr Biol 22:R128-30. 2012
    ..Using clever genetic experiments in the budding yeast, Lau and Murray define the endpoint of checkpoint signaling and provide key mechanistic insights into checkpoint inhibition of APC/C...
  54. doi request reprint Cohesin: a multi-purpose chromatin glue
    Laura A Diaz-Martinez
    Department of Pharmacology, Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    J Mol Cell Biol 1:58-60. 2009
    ..Recent discoveries point to cohesin having a role in transcription regulation by mediating long-distance intra-chromosomal interactions...
  55. doi request reprint Tracking spindle checkpoint signals from kinetochores to APC/C
    Luying Jia
    Howard Hughes Medical Institute, Department of Pharmacology, University of Texas Southwestern Medical Center, 6001 Forest Park Road, Dallas, TX 75390, USA
    Trends Biochem Sci 38:302-11. 2013
    ..Here, we review recent progress on the generation, propagation, transmission, and silencing of the spindle checkpoint signals from kinetochores to APC/C...
  56. pmc Nucleoporin levels regulate cell cycle progression and phase-specific gene expression
    Papia Chakraborty
    Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    Dev Cell 15:657-67. 2008
    ..Thus, Nup96 levels regulate differential gene expression in a phase-specific manner, setting the stage for proper cell cycle progression...
  57. pmc FWD1-mediated degradation of FREQUENCY in Neurospora establishes a conserved mechanism for circadian clock regulation
    Qun He
    Department of Physiology, The University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, TX 75390, USA
    EMBO J 22:4421-30. 2003
    ....
  58. ncbi request reprint Regulation of CDC14: pathways and checkpoints of mitotic exit
    Joshua Bembenek
    Department of Pharmacology, UT Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, TX 75390 9041, USA
    Front Biosci 8:d1275-87. 2003
    ..We review recent discoveries in several model systems that have shed light on the function of Cdc14 and propose a general framework within which Cdc14 plays conserved roles in regulating the exit from mitosis and cytokinesis...
  59. ncbi request reprint ATP binding and ATP hydrolysis play distinct roles in the function of 26S proteasome
    Chang wei Liu
    Department of Physiology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390, USA
    Mol Cell 24:39-50. 2006
    ..These results indicate that 26S proteasome-catalyzed degradation of polyubiquitylated proteins involves mechanistic coupling of several processes and that such coupling imposes an energy requirement not apparent for any isolated process...
  60. pmc Structural insights into histone lysine demethylation
    Haifeng Hou
    Howard Hughes Medical Institute, Department of Pharmacology, University of Texas Southwestern Medical Center, 6001 Forest Park Road, Dallas, TX 75390, USA
    Curr Opin Struct Biol 20:739-48. 2010
    ..Here, we review these exciting advances in the structure biology of histone demethylases and discuss the general principles applicable to other histone-modifying enzymes...
  61. ncbi request reprint The Transcription Factor TFII-I Promotes DNA Translesion Synthesis and Genomic Stability
    Farjana J Fattah
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America Simmons Comprehensive Cancer Center, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America
    PLoS Genet 10:e1004419. 2014
    ..Our findings extend the general principle of component sharing among divergent nuclear processes and implicate TLS deficiency as a possible contributing factor in Williams-Beuren syndrome. ..
  62. ncbi request reprint Cdh1 Is a HECT of an Activator
    Luying Jia
    Department of Pharmacology, Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, 6001 Forest Park Road, Dallas, TX 75390, USA
    Mol Cell 44:681-3. 2011
    ..In this issue of Molecular Cell, Wan et al. (2011) report an APC/C-independent role of Cdh1 during development as an activator for Smurf1, a HECT-type ubiquitin ligase...
  63. ncbi request reprint Kinetic and mechanistic studies of a cell cycle protein phosphatase Cdc14
    Wei Qing Wang
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Biol Chem 279:30459-68. 2004
    ..We also identified several residues including Asp50, Asp129, Glu168, Glu171, and Asp177 in the Cdc14 active site cleft that are required for efficient dephosphorylation of hCdh1...
  64. ncbi request reprint Self-regulated Plk1 recruitment to kinetochores by the Plk1-PBIP1 interaction is critical for proper chromosome segregation
    Young H Kang
    Laboratory of Metabolism, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland 20892, USA
    Mol Cell 24:409-22. 2006
    ..Thus, Plk1 self-regulates the Plk1-PBIP1 interaction to timely localize to the kinetochores and promote proper chromosome segregation...
  65. ncbi request reprint Two distinct pathways for inhibiting pds1 ubiquitination in response to DNA damage
    Ritu Agarwal
    Laboratory of Molecular and Cellular Biology, NIDDK, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 278:45027-33. 2003
    ..Finally, we show that once the DNA damage is repaired, Pds1 dephosphorylation is involved in the recovery from the checkpoint induced cell cycle arrest...
  66. pmc A requirement for breast-cancer-associated gene 1 (BRCA1) in the spindle checkpoint
    Rui Hong Wang
    National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 101:17108-13. 2004
    ..These data reveal a role of BRCA1 in maintaining genome integrity by interplaying with p53 and genes that are involved in the spindle checkpoint and apoptosis...
  67. pmc HTLV-I Tax directly binds the Cdc20-associated anaphase-promoting complex and activates it ahead of schedule
    Baoying Liu
    Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, MD 20814, USA
    Proc Natl Acad Sci U S A 102:63-8. 2005
    ..Unscheduled activation of APC(Cdc20) by Tax provides an explanation for the mitotic abnormalities in HTLV-I-infected cells and is likely to play an important role in the development of adult T cell leukemia...
  68. pmc A novel motif governs APC-dependent degradation of Drosophila ORC1 in vivo
    Marito Araki
    Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, North Carolina 27710, USA
    Genes Dev 19:2458-65. 2005
    ....
  69. pmc Degradation of origin recognition complex large subunit by the anaphase-promoting complex in Drosophila
    Marito Araki
    Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC 27710, USA
    EMBO J 22:6115-26. 2003
    ..These observations suggest that in Drosophila, ORC1 regulates origin utilization much as does Cdc6 in budding yeast...

Research Grants17

  1. Regulation of the Anaphase-Promoting Complex
    Hongtao Yu; Fiscal Year: 2001
    ..Research on the regulation of APCcdh1 will provide insights into how the mitotic checkpoint halts the cell cycle and present novel molecular targets for the design of agents that interfere with this pathway. ..
  2. Regulation of the Anaphase-Promoting Complex by the Spindle Checkpoint
    Hongtao Yu; Fiscal Year: 2009
    ..Experiments in this proposal will shed light on the molecular basis of the spindle checkpoint, which might eventually lead to new strategies to treat human cancers. ..
  3. Protection of Centromeric Cohesion by Bub1 and Sgo1
    Hongtao Yu; Fiscal Year: 2009
    ..abstract_text> ..
  4. Regulation of the Anaphase-Promoting Complex by the Spindle Checkpoint
    Hongtao Yu; Fiscal Year: 2010
    ..Experiments in this proposal will shed light on the molecular basis of the spindle checkpoint, which might eventually lead to new strategies to treat human cancers. ..
  5. Regulation of the Anaphase-Promoting Complex
    Hongtao Yu; Fiscal Year: 2002
    ..Research on the regulation of APCcdh1 will provide insights into how the mitotic checkpoint halts the cell cycle and present novel molecular targets for the design of agents that interfere with this pathway. ..
  6. Regulation of the Anaphase-Promoting Complex
    Hongtao Yu; Fiscal Year: 2005
    ..Research on the regulation of APCcdh1 will provide insights into how the mitotic checkpoint halts the cell cycle and present novel molecular targets for the design of agents that interfere with this pathway. ..
  7. Regulation of the Anaphase-Promoting Complex
    Hongtao Yu; Fiscal Year: 2004
    ..Research on the regulation of APCcdh1 will provide insights into how the mitotic checkpoint halts the cell cycle and present novel molecular targets for the design of agents that interfere with this pathway. ..
  8. Regulation of the Anaphase-Promoting Complex by the Spindle Checkpoint
    Hongtao Yu; Fiscal Year: 2006
    ..Experiments in this proposal will shed light on the molecular basis of the spindle checkpoint, which might eventually lead to new strategies to treat human cancers. ..
  9. Protection of Centromeric Cohesion by Bub1 and Sgo1
    Hongtao Yu; Fiscal Year: 2010
    ....
  10. Protection of Centromeric Cohesion by Bub1 and Sgo1
    Hongtao Yu; Fiscal Year: 2009
    ....
  11. Regulation of the Anaphase-Promoting Complex by the Spindle Checkpoint
    Hongtao Yu; Fiscal Year: 2009
    ..Experiments in this proposal will shed light on the molecular basis of the spindle checkpoint, which might eventually lead to new strategies to treat human cancers. ..
  12. Protection of Centromeric Cohesion by Bub1 and Sgo1
    Hongtao Yu; Fiscal Year: 2007
    ....
  13. Regulation of the Anaphase-Promoting Complex by the Spindle Checkpoint
    Hongtao Yu; Fiscal Year: 2007
    ..Experiments in this proposal will shed light on the molecular basis of the spindle checkpoint, which might eventually lead to new strategies to treat human cancers. ..
  14. Regulation of the Anaphase-Promoting Complex
    Hongtao Yu; Fiscal Year: 2003
    ..Research on the regulation of APCcdh1 will provide insights into how the mitotic checkpoint halts the cell cycle and present novel molecular targets for the design of agents that interfere with this pathway. ..