Research Topics
Genomes and Genes | ELTON YOUNGSummaryAffiliation: University of Washington Country: USA Publications
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Publications
Hyperacetylation of chromatin at the ADH2 promoter allows Adr1 to bind in repressed conditionsLoredana Verdone
Fondazione Istituto Pasteur Fondazione Cenci Bolognetti and Centro di Studio per gli Acidi Nucleici, CNR, c o Dipartimento di Genetica e Biologia Molecolare, Universita La Sapienza, I 00185 Rome, Italy
EMBO J 21:1101-11. 2002....- Adr1 and Cat8 mediate coactivator recruitment and chromatin remodeling at glucose-regulated genesRhiannon K Biddick
Department of Biochemistry, University of Washington, Seattle, Washington, United States of America
PLoS ONE 3:e1436. 2008..Adr1 and Cat8 co-regulate numerous glucose-repressed genes in S. cerevisiae, presenting a unique opportunity to explore their individual roles in coactivator recruitment, chromatin remodeling, and transcription... 
Multiple pathways are co-regulated by the protein kinase Snf1 and the transcription factors Adr1 and Cat8Elton T Young
Department of Biochemistry, The University of Washington, Seattle, Washington 98195 7350, USA
J Biol Chem 278:26146-58. 2003..The largest class of SNF1-dependent genes encodes regulatory proteins that could extend SNF1 dependence to additional pathways...- Trinucleotide repeats are clustered in regulatory genes in Saccharomyces cerevisiaeE T Young
Department of Biochemistry, University of Washington, Seattle, Washington 98195 7350, USA
Genetics 154:1053-68. 2000..Specifically, TNRs could serve as hot spots for recombination leading to domain swapping, or mutation of TNRs could allow rapid evolution of new domains of protein structure... - Evolution of a glucose-regulated ADH gene in the genus SaccharomycesE T Young
Department of Biochemistry, University of Washington, Seattle, WA, USA
Gene 245:299-309. 2000..cerevisiae and S. bayanus. No ADR1 homologue could be detected in S. kluyveri, suggesting that the potential for Adr1 regulation may have arisen first, before ADR1 evolved... - An accessory DNA binding motif in the zinc finger protein Adr1 assists stable binding to DNA and can be replaced by a third fingerE T Young
Department of Biochemistry, Box 357350, University of Washington, Seattle, Washington 98195 7350, USA
Biochemistry 39:567-74. 2000..In addition, PAR may have a role in transcription at a step after DNA binding since deletion of PAR from Adr1 with three fingers decreased activation in vivo but had no effect on DNA binding kinetics... - Snf1 protein kinase regulates Adr1 binding to chromatin but not transcription activationElton T Young
Department of Biochemistry, University of Washington, Seattle, Washington 98195 7350, USA
J Biol Chem 277:38095-103. 2002..We conclude that Snf1 regulates Adr1-dependent transcription primarily at the level of chromatin binding... - Characterization of a p53-related activation domain in Adr1p that is sufficient for ADR1-dependent gene expressionE T Young
Department of Biochemistry, University of Washington, Seattle, Washington 98195 7350, USA
J Biol Chem 273:32080-7. 1998..Thus, a single activation domain can activate all ADR1-dependent promoters... - Artificial recruitment of mediator by the DNA-binding domain of Adr1 overcomes glucose repression of ADH2 expressionElton T Young
Department of Biochemistry, Box 357350, University of Washington, Seattle, WA 98195 7350, USA
Mol Cell Biol 28:2509-16. 2008..Artificial recruitment of Mediator appears to overcome this requirement and to allow stable binding and transcription under normally inhibitory conditions... 
Snf1-independent, glucose-resistant transcription of Adr1-dependent genes in a mediator mutant of Saccharomyces cerevisiaeElton T Young
Department of Biochemistry, University of Washington, Seattle, WA, USA
Mol Microbiol 74:364-83. 2009..Expression of most genes was not significantly affected by the loss of Med14 function...- Post-translational regulation of Adr1 activity is mediated by its DNA binding domainJ S Sloan
Department of Biochemistry, University of Washington, Seattle, Washington 98195 7350, USA
J Biol Chem 274:37575-82. 1999..A Gal4-Adr1 fusion protein was unable to confer glucose repression at GAL4-dependent promoters, suggesting that regulation mediated by ADR1 is specific to UAS1... - ADH2 expression is repressed by REG1 independently of mutations that alter the phosphorylation of the yeast transcription factor ADR1K M Dombek
Department of Biochemistry, University of Washington, Seattle 98195
Mol Cell Biol 13:4391-9. 1993..Our results suggest that glucose repression in the presence of ADR1 constitutive alleles occurs primarily through a REG1-dependent pathway which appears to affect ADH2 transcription at multiple levels... - Isolation and identification of genes activating UAS2-dependent ADH2 expression in Saccharomyces cerevisiaeM S Donoviel
Department of Biochemistry, University of Washington, Seattle 98195, USA
Genetics 143:1137-48. 1996..A MEU1/beta-gal fusion protein was not localized to a particular region of the cell. MEU1 is linked to PPR1 on chromosome XII... - 14-3-3 (Bmh) proteins inhibit transcription activation by Adr1 through direct binding to its regulatory domainP K Parua
Department of Biochemistry, University of Washington, 1705 NE Pacific St, Seattle, WA 98195 7350, USA
Mol Cell Biol 30:5273-83. 2010..The Bmh binding domain is conserved in orthologs of Adr1, suggesting that it acquired an important biological function before the whole-genome duplication of the ancestor of S. cerevisiae... - Identification and characterization of three genes that affect expression of ADH2 in Saccharomyces cerevisiaeL Karnitz
Department of Biochemistry, University of Washington, Seattle 98195
Genetics 132:351-9. 1992..The mutations were shown to be nonallelic to a wide variety of previously characterized mutations, including mutations that affect other glucose-repressed enzymes... - Functional analysis of the yeast Glc7-binding protein Reg1 identifies a protein phosphatase type 1-binding motif as essential for repression of ADH2 expressionK M Dombek
Department of Biochemistry, University of Washington, Seattle, Washington 98195 7350, USA
Mol Cell Biol 19:6029-40. 1999..This implies that the activated form of the Snf1 kinase complex must cycle between the nucleus and the cytoplasm... - Cyclic AMP-dependent protein kinase inhibits ADH2 expression in part by decreasing expression of the transcription factor gene ADR1K M Dombek
Department of Biochemistry, University of Washington, Seattle 98195 7350, USA
Mol Cell Biol 17:1450-8. 1997..Overexpression of Adr1p only partially restored ADH2 expression, indicating that some of these mechanisms may impinge upon events at or subsequent to the ADR1-dependent step in ADH2 transcriptional activation... - Meiotic inheritance of functional Gal80S gene product in Saccharomyces cerevisiaeA D Keller
Department of Biochemistry, University of Washington, Seattle 98195, USA
Yeast 13:441-7. 1997..We show that functional Gal80S repressor is meiotically inherited at levels reflecting its expression in the diploid parent... - A poised initiation complex is activated by SNF1Christine Tachibana
Department of Biochemistry, University of Washington, Seattle, WA 98195, USA
J Biol Chem 282:37308-15. 2007.... - Snf1-dependent and Snf1-independent pathways of constitutive ADH2 expression in Saccharomyces cerevisiaeValentina Voronkova
Department of Biochemistry, University of Washington, Seattle, WA 98195 7350, USA
Genetics 172:2123-38. 2006..Thus, the mutant search revealed previously unknown Snf1-dependent and -independent pathways of ADH2 expression... - Yeast mediator and its role in transcriptional regulationRhiannon Biddick
Department of Biochemistry, University of Washington, Box 357350, Seattle, WA 98195-7350, USA
C R Biol 328:773-82. 2005..One focal point of the review is to summarize new information regarding the negative effect of Mediator on transcription and suggest a possible mechanism that encompasses the latest results... - Combined global localization analysis and transcriptome data identify genes that are directly coregulated by Adr1 and Cat8Christine Tachibana
Department of Biochemistry, Box 357350, University of Washington, Seattle, WA 98195 7350, USA
Mol Cell Biol 25:2138-46. 2005..These data provide a comprehensive analysis of the direct, indirect, and combinatorial requirements for these two global transcription factors... - The Reg1-interacting proteins, Bmh1, Bmh2, Ssb1, and Ssb2, have roles in maintaining glucose repression in Saccharomyces cerevisiaeKenneth M Dombek
Department of Biochemistry, University of Washington, Seattle, Washington 98195 7350, USA
J Biol Chem 279:39165-74. 2004..Deletion of both SSB genes modestly decreases repression of ADH2 expression in the presence of glucose, suggesting that Ssb proteins, perhaps through their interaction with Reg1, play a minor role in glucose repression... - Genome-wide amplifications caused by chromosomal rearrangements play a major role in the adaptive evolution of natural yeastJuan J Infante
, CASEM, , 11510 Puerto Real, , Spain
Genetics 165:1745-59. 2003..This mechanism of chromosomal evolution could also account for the sexual isolation shown among the flor yeast... - Cyclic changes in metabolic state during the life of a yeast cellBenjamin P Tu
Department of Biochemistry, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390 9038, USA
Proc Natl Acad Sci U S A 104:16886-91. 2007..Such oscillations in metabolic state might occur during the course of other biological cycles... - Identification and evaluation of cycling yeast metabolites in two-dimensional comprehensive gas chromatography-time-of-flight-mass spectrometry dataRachel E Mohler
University of Washington, Department of Chemistry, Box 351700, Seattle, WA 98195, USA
J Chromatogr A 1186:401-11. 2008..The phase delay of the cycling metabolites ranged widely in relation to the oxygen consumption cycling pattern... - Comprehensive two-dimensional gas chromatography time-of-flight mass spectrometry analysis of metabolites in fermenting and respiring yeast cellsRachel E Mohler
Department of Chemistry, University of Washington, Box 351700, Seattle, Washington 98195, USA
Anal Chem 78:2700-9. 2006....
Research Grants
- GENETIC REGULATION OF ALCOHOL METABOLISM IN YEASTElton T Young; Fiscal Year: 2010..This information in turn, might lead to new insights into treatment and diagnosis of metabolic disorders brought about by pathological conditions related to glucose metabolism. ..
- GENETIC REGULATION OF ALCOHOL METABOLISM IN YEASTELTON YOUNG; Fiscal Year: 2009....
- GENETIC REGULATION OF ALCOHOL METABOLISM IN YEASTELTON YOUNG; Fiscal Year: 2003..Thus, the proposed specific aims relate to these aspects of the structure and function of Adr1p. ..
- Transcriptional Integration of MetabolismELTON YOUNG; Fiscal Year: 2007..abstract_text> ..
- GENETIC REGULATION OF ALCOHOL METABOLISM IN YEASTELTON YOUNG; Fiscal Year: 1999..Thus, our specific aims relate to these aspects of the structure and function of Adr1p. ..
- GENETIC REGULATION OF ALCOHOL METABOLISM IN YEASTELTON YOUNG; Fiscal Year: 1991..A basic understanding of how proteins control gene action will ultimately be of medical benefit as well...
- GENETIC REGULATION OF ALCOHOL METABOLISM IN YEASTELTON YOUNG; Fiscal Year: 1993..Since several human diseases have been identified that are due to mutations in zinc finger transcription factors, understanding how these factors function is useful to an understanding of the defect in these diseases...
- GENETIC REGULATION OF ALCOHOL METABOLISM IN YEASTELTON YOUNG; Fiscal Year: 1980..The effect of various mutations on the levels of ADH mRNA and proteins will be characterized in an effort to understand the regulation of ADH gene expression. ..