Research Topics
| Paul YagerSummaryAffiliation: University of Washington Country: USA Publications
Research Grants
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Detail Information
Publications
Nonlinear decrease of background fluorescence in polymer thin-films - a survey of materials and how they can complicate fluorescence detection in microTASKenneth R Hawkins
Department of Bioengineering, University of Washington, Seattle, Washington, USA
Lab Chip 3:248-52. 2003..We then discuss how this has confounded fluorescence detection in our analytical context, and possible mechanisms for the decrease...- Microfluidic diagnostic technologies for global public healthPaul Yager
Department of Bioengineering, Box 355061, University of Washington, Seattle, Washington 98195, USA
Nature 442:412-8. 2006..These systems must be inexpensive, but also accurate, reliable, rugged and well suited to the medical and social contexts of the developing world... 
Point-of-care diagnostics for global healthPaul Yager
Department of Bioengineering, University of Washington, Seattle, WA 98195 5061, USA
Annu Rev Biomed Eng 10:107-44. 2008..However, there is much room for innovation, adaptation, and cost reduction before these technologies can impact health care in the developing world...- Multiplexed enrichment and detection of malarial biomarkers using a stimuli-responsive iron oxide and gold nanoparticle reagent systemMichael A Nash
Department of Bioengineering, University of Washington, Seattle, Washington 98195, USA
ACS Nano 6:6776-85. 2012.... 
Mixed stimuli-responsive magnetic and gold nanoparticle system for rapid purification, enrichment, and detection of biomarkersMichael A Nash
Department of Bioengineering, University of Washington, Seattle, Washington 98195, USA
Bioconjug Chem 21:2197-204. 2010....- Laboratory-scale protein striping system for patterning biomolecules onto paper-based immunochromatographic test stripsMichael A Nash
University of Washington, Department of Bioengineering, Box 355061, 3720 15th Ave NE, Seattle, WA 98195 5061, USA
Lab Chip 10:2279-82. 2010..This method consumes very small volumes of reagents, and is a viable option for laboratory-scale fabrication and prototyping of point-of-care rapid diagnostic test strips... - Two-dimensional paper network format that enables simple multistep assays for use in low-resource settings in the context of malaria antigen detectionElain Fu
Department of Bioengineering, University of Washington, Seattle, Washington, United States
Anal Chem 84:4574-9. 2012..These results highlight the potential of the paper network platform to enhance access to high-quality diagnostic capabilities in low-resource settings in the developed and developing worlds... - "Smart" diblock copolymers as templates for magnetic-core gold-shell nanoparticle synthesisMichael A Nash
Department of Bioengineering, University of Washington, Seattle, WA 98195, USA
Nano Lett 10:85-91. 2010..The Au-mNPs reversibly aggregated upon heating the solution above the transition temperature of pNIPAAm, resulting in a red-shifted localized surface plasmon resonance... - Experimental and model investigation of the time-dependent 2-dimensional distribution of binding in a herringbone microchannelJennifer O Foley
Department of Bioengineering, University of Washington, Seattle, WA, USA
Lab Chip 8:557-64. 2008..5 mm) down the length of the microchannel, the model predicted no additional protein binding in the herringbone microchannel compared to the straight microchannel, consistent with previous findings in the literature... - Modeling of a competitive microfluidic heterogeneous immunoassay: sensitivity of the assay response to varying system parametersElain Fu
Department of Bioengineering, University of Washington, Seattle, Washington 98195, USA
Anal Chem 81:3407-13. 2009..Experimental assay results for the drug phenytoin, obtained using surface plasmon resonance imaging, are shown to be consistent with the predictions of the model... - Investigation of heterogeneous electrochemical processes using multi-stream laminar flow in a microchannelMelissa S Hasenbank
Department of Bioengineering, University of Washington, Seattle, WA 98195, USA
Lab Chip 7:441-7. 2007..This improved understanding of the underlying mechanism should enable the future implementation of this phenomenon in signal amplification schemes for highly sensitive lab-on-a-chip biosensors... - A method for characterizing adsorption of flowing solutes to microfluidic device surfacesKenneth R Hawkins
Department of Bioengineering, University of Washington, Seattle, WA, USA
Lab Chip 7:281-5. 2007.... - Microfluidics without pumps: reinventing the T-sensor and H-filter in paper networksJennifer L Osborn
Department of Bioengineering, University of Washington, Box 355061, Seattle, WA 98195, USA
Lab Chip 10:2659-65. 2010.... - Microcontact printed antibodies on gold surfaces: function, uniformity, and silicone contaminationJennifer O Foley
Department of Bioengineering and National ESCA and Surface Analysis Center for Biomedical Problems, University of Washington, Seattle, Washington 98195, USA
Langmuir 24:3628-35. 2008..The results suggest that the silicone oligomer introduced by the printing process reduces the overall binding capacity of the microcontact-printed protein compared to physically adsorbed protein... - Visualization and measurement of flow in two-dimensional paper networksPeter Kauffman
Department of Bioengineering, University of Washington, Box 355061, Seattle, WA, USA
Lab Chip 10:2614-7. 2010..In this note, we demonstrate two methods for visualization of flow in 2DPNs that are inexpensive, easy to implement, and quantifiable... - Conditioning saliva for use in a microfluidic biosensorKristen L Helton
Department of Bioengineering, University of Washington, Seattle, WA 98195, USA
Lab Chip 8:1847-51. 2008..6 times less surface fouling than either untreated or filtered saliva alone. These sample conditioning steps will enable the use of fouling-sensitive detection techniques in future studies using clinical saliva samples... - Demonstration of multi-analyte patterning using piezoelectric inkjet printing of multiple layersMelissa S Hasenbank
Department of Bioengineering, University of Washington, Seattle, WA 98195, United States
Anal Chim Acta 611:80-8. 2008..Thus, this method produces high-quality, localized, and customizable sub-millimeter patterns in a nonfouling background for multi-analyte bioassay development... - Progress toward multiplexed sample-to-result detection in low resource settings using microfluidic immunoassay cardsLisa Lafleur
Department of Electrical Engineering, University of Washington, Seattle, WA, USA
Lab Chip 12:1119-27. 2012..All reagents were stored on card in dry form; only the sample and buffer were required to run the tests. Here we detail the development of this platform and discuss its strengths and weaknesses... - Two-dimensional paper networks: programmable fluidic disconnects for multi-step processes in shaped paperBarry R Lutz
Department of Bioengineering, University of Washington, Box 355061, Seattle, WA, USA
Lab Chip 11:4274-8. 2011..This approach could enable multi-step laboratory assays to be converted into simple point-of-care devices that have high performance yet remain easy to use... - Concentration gradient immunoassay. 2. Computational modeling for analysis and optimizationJennifer O Foley
Department of Bioengineering, and Department of Chemical Engineering, University of Washington, Seattle, Washington 98195, USA
Anal Chem 79:3549-53. 2007..Given the experimental verification of the computational results, this model serves as an efficient method to explore the influence of the flow rate, microchannel dimensions, and antibody concentration on the sensitivity of the assay... - SPR imaging-based salivary diagnostics system for the detection of small molecule analytesElain Fu
Department of Bioengineering, University of Washington, Seattle, WA 98195, USA
Ann N Y Acad Sci 1098:335-44. 2007..The multicomponent system includes a hand-portable surface plasmon resonance (SPR) imaging instrument and a disposable microfluidic assay card... - Dynamic bioprocessing and microfluidic transport control with smart magnetic nanoparticles in laminar-flow devicesJames J Lai
Department of Bioengineering, University of Washington, Seattle, WA 98195, USA
Lab Chip 9:1997-2002. 2009..This stimuli-responsive reagent system has been shown to transfer 81% of a model protein target from an input flowstream to a second flowstream in a continuous flow H-filter device... - Concentration gradient immunoassay. 1. An immunoassay based on interdiffusion and surface binding in a microchannelKjell E Nelson
Department of Bioengineering, University of Washington, Seattle, Washington 98195, USA
Anal Chem 79:3542-8. 2007..This assay method is suitable for rapid quantitative detection of low molecular weight analytes for point-of-care diagnostic instrumentation... - Enhanced sensitivity of lateral flow tests using a two-dimensional paper network formatElain Fu
Box 355061, Department of Bioengineering, University of Washington, Seattle, Washington, USA
Anal Chem 83:7941-6. 2011..These results highlight the potential of the paper network platform to enhance access to high-quality diagnostic capabilities in low-resource settings in the developed and developing worlds... - Rapid protein depletion from complex samples using a bead-based microfluidic device for the point of careKatherine G McKenzie
Department of Bioengineering, University of Washington, Box 355061, Foege N530J, Seattle, WA 98195 5061, USA
Lab Chip 9:3543-8. 2009..This design can easily be adapted for depletion of other high abundance or interfering proteins by inclusion of other ligand immobilized beads... - High-throughput screening of enzyme inhibition using an inhibitor gradient generated in a microchannelElena Garcia
Department of Bioengineering, University of Washington, Box 355061, Foege N530J, 1705 NE Pacific Street, Seattle, WA 98195 2255, USA
Lab Chip 7:249-55. 2007..Recommendations for use of the assay are provided, including its use for high-throughput screening in drug discovery and general use in measurement of enzyme inhibition... - Dependence of the signal amplification potential of colloidal gold nanoparticles on resonance wavelength in surface plasmon resonance-based detectionElain Fu
Department of Bioengineering, University of Washington, Seattle, WA 98195, United States
Anal Chim Acta 599:118-23. 2007..The results demonstrate an almost twofold signal difference for a subset of the experimental conditions explored... - Passive electrophoresis in microchannels using liquid junction potentialsMatthew S Munson
Department of Bioengineering, University of Washington, Box 352141, Seattle, WA 98195, USA
Electrophoresis 23:2642-52. 2002..Possible practical microfluidic applications of electrophoretic transport without electrodes are discussed... - Enabling a microfluidic immunoassay for the developing world by integration of on-card dry reagent storageDean Y Stevens
Department of Bioengineering, University of Washington, Box 355061, Foege N530J, 1705 NE Pacific Street, Seattle, WA 98195 5061, USA
Lab Chip 8:2038-45. 2008..The system gave a detection limit in the sub-nanomolar range in under nine minutes, showing the potential to expand into quantitative, multi-analyte analysis of human blood samples... - Lateral spread of an amplification signal using an enzymatic system on a conductive surfaceMelissa S Hasenbank
University of Washington, Department of Bioengineering, Box 355061, Seattle, Washington 98195, USA
Langmuir 22:7451-3. 2006.... - Imaging of surfaces by concurrent surface plasmon resonance and surface plasmon resonance-enhanced fluorescenceRahber Thariani
Department of Bioengineering, University of Washington, Seattle, Washington, United States of America
PLoS ONE 5:e9833. 2010..The instrument allows for high performance imaging and quantitative measurements with surface plasmon resonance, and surface plasmon induced fluorescence, with inexpensive off-the-shelf components... - A practical guide to the staggered herringbone mixerManda S Williams
Department of Bioengineering, University of Washington, Seattle, WA 98195, USA
Lab Chip 8:1121-9. 2008..This analytical model is applicable to other solute systems, and possibly to other embodiments of the mixer, to enable optimal design, operation, and estimation of performance... - Controlled reagent transport in disposable 2D paper networksElain Fu
Box 355061, Department of Bioengineering, University of Washington, Seattle, WA, USA
Lab Chip 10:918-20. 2010..Specifically, we demonstrate methods of controlling fluid transport using the geometry of the network and dissolvable barriers. Finally, we discuss the implications for higher sensitivity detection using this type of 2D paper network... - Diffusion based analysis in a sheath flow microchannel: the sheath flow T-sensorMatthew S Munson
University of Washington, Department of Bioengineering, Box 352255, Seattle, WA, USA
Lab Chip 5:856-62. 2005..Measurements for both a small molecule (rhodamine B) and an intermediate-sized protein (wheat germ agglutinin) were made, demonstrating the utility of the sheath flow T-sensor... - Controlled release of dry reagents in porous media for tunable temporal and spatial distribution upon rehydrationGina E Fridley
Department of Bioengineering, University of Washington, Seattle, WA 98195, USA
Lab Chip 12:4321-7. 2012..Finally, the utility of these control methods is demonstrated in the context of real-time reagent rehydration and mixing on a porous device... - Interfacial instabilities affect microfluidic extraction of small molecules from non-Newtonian fluidsKristen L Helton
Department of Bioengineering, University of Washington, 1705 NE Pacific Street, Seattle, WA 98195, USA
Lab Chip 7:1581-8. 2007.... - Recirculating flow accelerates DNA microarray hybridization in a microfluidic deviceHyun Ho Lee
Department of Bioengineering, University of Washington, Seattle, WA 98195, USA
Lab Chip 6:1163-70. 2006..54 degrees C, relative to static conditions. The experimental results were consistent with a three-dimensional simulation of hybridization using a recirculating buffer system... - Suppression of non-specific adsorption using sheath flowMatthew S Munson
University of Washington, Box 352141, Seattle, WA, USA
Lab Chip 4:438-45. 2004..The controlled adsorption of the protein bovine serum albumin (BSA) to a gold surface is also demonstrated using SPR microscopy... - Controlled microfluidic reconstitution of functional protein from an anhydrous storage depotElena Garcia
Department of Bioengineering, University of Washington, Box 352141, Seattle, WA 98195-2255, USA
Lab Chip 4:78-82. 2004..More broadly, this preservation and controlled release technique can be applied where the preservation and/or spatial and temporal control of chemical concentrations are desired... - A smart microfluidic affinity chromatography matrix composed of poly(N-isopropylacrylamide)-coated beadsNoah Malmstadt
Department of Bioengineering, University of Washington, Seattle, Washington 98195, USA
Anal Chem 75:2943-9. 2003..This temperature-responsive affinity chromatography matrix can thus be flowed into a column and aggregated via temperature change, followed by the controlled release of affinity-captured targets back into the microfluidic flow stream... - Cell lysis and protein extraction in a microfluidic device with detection by a fluorogenic enzyme assayEric A Schilling
Department of Bioengineering, University of Washington, Seattle 98195, USA
Anal Chem 74:1798-804. 2002..While the focus is on the lysis of typical gram-negative bacterial cells (E. coli), the techniques described here could, in principle, be applied to a variety of different cell types... - Cross-talk problem on a fluorescence multi-channel microfluidic chip systemRudi Irawan
Biomedical Engineering Research Centre, School of Electrical and Electronic Engineering, Nanyang Technological University, Singapore 637553
Biomed Microdevices 7:205-11. 2005..We monitored this cross-talk using fluorescein as a fluorescent sample and Mylar sheeting as a microfluidic chip material. We then discuss how this cross-talk can be avoided using a simple, inexpensive and effective method... - Compact, high performance surface plasmon resonance imaging systemTimothy M Chinowsky
Biosens Bioelectron 22:2208-15. 2007..The simple, compact construction and high performance of the imager will allow the device to be readily applied to a wide range of applications...
Research Grants
- A Microfluidic Diffusion ImmunoassayPaul Yager; Fiscal Year: 2004..abstract_text> ..
- Rapid parallel salivary immunoassays on a disposablePaul Yager; Fiscal Year: 2005..Finally, a versatile combined system will be designed and tested that will allow monitoring of samples by the two methods simultaneously. ..