Paul Yager

Summary

Affiliation: University of Washington
Country: USA

Publications

  1. ncbi request reprint Microfluidic diagnostic technologies for global public health
    Paul Yager
    Department of Bioengineering, Box 355061, University of Washington, Seattle, Washington 98195, USA
    Nature 442:412-8. 2006
  2. ncbi request reprint Nonlinear decrease of background fluorescence in polymer thin-films - a survey of materials and how they can complicate fluorescence detection in microTAS
    Kenneth R Hawkins
    Department of Bioengineering, University of Washington, Seattle, Washington, USA
    Lab Chip 3:248-52. 2003
  3. doi request reprint Point-of-care diagnostics for global health
    Paul Yager
    Department of Bioengineering, University of Washington, Seattle, WA 98195 5061, USA
    Annu Rev Biomed Eng 10:107-44. 2008
  4. pmc Multiplexed enrichment and detection of malarial biomarkers using a stimuli-responsive iron oxide and gold nanoparticle reagent system
    Michael A Nash
    Department of Bioengineering, University of Washington, Seattle, Washington 98195, USA
    ACS Nano 6:6776-85. 2012
  5. pmc Mixed stimuli-responsive magnetic and gold nanoparticle system for rapid purification, enrichment, and detection of biomarkers
    Michael A Nash
    Department of Bioengineering, University of Washington, Seattle, Washington 98195, USA
    Bioconjug Chem 21:2197-204. 2010
  6. pmc Laboratory-scale protein striping system for patterning biomolecules onto paper-based immunochromatographic test strips
    Michael A Nash
    University of Washington, Department of Bioengineering, Box 355061, 3720 15th Ave NE, Seattle, WA 98195 5061, USA
    Lab Chip 10:2279-82. 2010
  7. pmc Dissolvable fluidic time delays for programming multi-step assays in instrument-free paper diagnostics
    Barry Lutz
    University of Washington, Department of Bioengineering, 3720 15th Ave NE, Box 355061, Seattle, WA 98195, USA
    Lab Chip 13:2840-7. 2013
  8. pmc Two-dimensional paper network format that enables simple multistep assays for use in low-resource settings in the context of malaria antigen detection
    Elain Fu
    Department of Bioengineering, University of Washington, Seattle, Washington, United States
    Anal Chem 84:4574-9. 2012
  9. pmc "Smart" diblock copolymers as templates for magnetic-core gold-shell nanoparticle synthesis
    Michael A Nash
    Department of Bioengineering, University of Washington, Seattle, WA 98195, USA
    Nano Lett 10:85-91. 2010
  10. doi request reprint Experimental and model investigation of the time-dependent 2-dimensional distribution of binding in a herringbone microchannel
    Jennifer O Foley
    Department of Bioengineering, University of Washington, Seattle, WA, USA
    Lab Chip 8:557-64. 2008

Research Grants

  1. A Microfluidic Diffusion Immunoassay
    Paul Yager; Fiscal Year: 2001
  2. A Microfluidic Diffusion Immunoassay
    Paul Yager; Fiscal Year: 2002
  3. A Microfluidic Diffusion Immunoassay
    Paul Yager; Fiscal Year: 2003
  4. A Microfluidic Diffusion Immunoassay
    Paul Yager; Fiscal Year: 2004
  5. Rapid parallel salivary immunoassays on a disposable
    Paul Yager; Fiscal Year: 2005

Collaborators

Detail Information

Publications47

  1. ncbi request reprint Microfluidic diagnostic technologies for global public health
    Paul Yager
    Department of Bioengineering, Box 355061, University of Washington, Seattle, Washington 98195, USA
    Nature 442:412-8. 2006
    ..These systems must be inexpensive, but also accurate, reliable, rugged and well suited to the medical and social contexts of the developing world...
  2. ncbi request reprint Nonlinear decrease of background fluorescence in polymer thin-films - a survey of materials and how they can complicate fluorescence detection in microTAS
    Kenneth R Hawkins
    Department of Bioengineering, University of Washington, Seattle, Washington, USA
    Lab Chip 3:248-52. 2003
    ..We then discuss how this has confounded fluorescence detection in our analytical context, and possible mechanisms for the decrease...
  3. doi request reprint Point-of-care diagnostics for global health
    Paul Yager
    Department of Bioengineering, University of Washington, Seattle, WA 98195 5061, USA
    Annu Rev Biomed Eng 10:107-44. 2008
    ..However, there is much room for innovation, adaptation, and cost reduction before these technologies can impact health care in the developing world...
  4. pmc Multiplexed enrichment and detection of malarial biomarkers using a stimuli-responsive iron oxide and gold nanoparticle reagent system
    Michael A Nash
    Department of Bioengineering, University of Washington, Seattle, Washington 98195, USA
    ACS Nano 6:6776-85. 2012
    ....
  5. pmc Mixed stimuli-responsive magnetic and gold nanoparticle system for rapid purification, enrichment, and detection of biomarkers
    Michael A Nash
    Department of Bioengineering, University of Washington, Seattle, Washington 98195, USA
    Bioconjug Chem 21:2197-204. 2010
    ....
  6. pmc Laboratory-scale protein striping system for patterning biomolecules onto paper-based immunochromatographic test strips
    Michael A Nash
    University of Washington, Department of Bioengineering, Box 355061, 3720 15th Ave NE, Seattle, WA 98195 5061, USA
    Lab Chip 10:2279-82. 2010
    ..This method consumes very small volumes of reagents, and is a viable option for laboratory-scale fabrication and prototyping of point-of-care rapid diagnostic test strips...
  7. pmc Dissolvable fluidic time delays for programming multi-step assays in instrument-free paper diagnostics
    Barry Lutz
    University of Washington, Department of Bioengineering, 3720 15th Ave NE, Box 355061, Seattle, WA 98195, USA
    Lab Chip 13:2840-7. 2013
    ..The cards are capable of automating multi-step assay protocols normally used in laboratories, but in a rapid, low-cost, and easy-to-use format...
  8. pmc Two-dimensional paper network format that enables simple multistep assays for use in low-resource settings in the context of malaria antigen detection
    Elain Fu
    Department of Bioengineering, University of Washington, Seattle, Washington, United States
    Anal Chem 84:4574-9. 2012
    ..These results highlight the potential of the paper network platform to enhance access to high-quality diagnostic capabilities in low-resource settings in the developed and developing worlds...
  9. pmc "Smart" diblock copolymers as templates for magnetic-core gold-shell nanoparticle synthesis
    Michael A Nash
    Department of Bioengineering, University of Washington, Seattle, WA 98195, USA
    Nano Lett 10:85-91. 2010
    ..The Au-mNPs reversibly aggregated upon heating the solution above the transition temperature of pNIPAAm, resulting in a red-shifted localized surface plasmon resonance...
  10. doi request reprint Experimental and model investigation of the time-dependent 2-dimensional distribution of binding in a herringbone microchannel
    Jennifer O Foley
    Department of Bioengineering, University of Washington, Seattle, WA, USA
    Lab Chip 8:557-64. 2008
    ..5 mm) down the length of the microchannel, the model predicted no additional protein binding in the herringbone microchannel compared to the straight microchannel, consistent with previous findings in the literature...
  11. doi request reprint Modeling of a competitive microfluidic heterogeneous immunoassay: sensitivity of the assay response to varying system parameters
    Elain Fu
    Department of Bioengineering, University of Washington, Seattle, Washington 98195, USA
    Anal Chem 81:3407-13. 2009
    ..Experimental assay results for the drug phenytoin, obtained using surface plasmon resonance imaging, are shown to be consistent with the predictions of the model...
  12. ncbi request reprint Investigation of heterogeneous electrochemical processes using multi-stream laminar flow in a microchannel
    Melissa S Hasenbank
    Department of Bioengineering, University of Washington, Seattle, WA 98195, USA
    Lab Chip 7:441-7. 2007
    ..This improved understanding of the underlying mechanism should enable the future implementation of this phenomenon in signal amplification schemes for highly sensitive lab-on-a-chip biosensors...
  13. ncbi request reprint A method for characterizing adsorption of flowing solutes to microfluidic device surfaces
    Kenneth R Hawkins
    Department of Bioengineering, University of Washington, Seattle, WA, USA
    Lab Chip 7:281-5. 2007
    ....
  14. pmc Controlled release of dry reagents in porous media for tunable temporal and spatial distribution upon rehydration
    Gina E Fridley
    Department of Bioengineering, University of Washington, Seattle, WA 98195, USA
    Lab Chip 12:4321-7. 2012
    ..Finally, the utility of these control methods is demonstrated in the context of real-time reagent rehydration and mixing on a porous device...
  15. doi request reprint Visualization and measurement of flow in two-dimensional paper networks
    Peter Kauffman
    Department of Bioengineering, University of Washington, Box 355061, Seattle, WA, USA
    Lab Chip 10:2614-7. 2010
    ..In this note, we demonstrate two methods for visualization of flow in 2DPNs that are inexpensive, easy to implement, and quantifiable...
  16. doi request reprint Microcontact printed antibodies on gold surfaces: function, uniformity, and silicone contamination
    Jennifer O Foley
    Department of Bioengineering and National ESCA and Surface Analysis Center for Biomedical Problems, University of Washington, Seattle, Washington 98195, USA
    Langmuir 24:3628-35. 2008
    ..The results suggest that the silicone oligomer introduced by the printing process reduces the overall binding capacity of the microcontact-printed protein compared to physically adsorbed protein...
  17. doi request reprint Microfluidics without pumps: reinventing the T-sensor and H-filter in paper networks
    Jennifer L Osborn
    Department of Bioengineering, University of Washington, Box 355061, Seattle, WA 98195, USA
    Lab Chip 10:2659-65. 2010
    ....
  18. doi request reprint Conditioning saliva for use in a microfluidic biosensor
    Kristen L Helton
    Department of Bioengineering, University of Washington, Seattle, WA 98195, USA
    Lab Chip 8:1847-51. 2008
    ..6 times less surface fouling than either untreated or filtered saliva alone. These sample conditioning steps will enable the use of fouling-sensitive detection techniques in future studies using clinical saliva samples...
  19. doi request reprint Demonstration of multi-analyte patterning using piezoelectric inkjet printing of multiple layers
    Melissa S Hasenbank
    Department of Bioengineering, University of Washington, Seattle, WA 98195, United States
    Anal Chim Acta 611:80-8. 2008
    ..Thus, this method produces high-quality, localized, and customizable sub-millimeter patterns in a nonfouling background for multi-analyte bioassay development...
  20. doi request reprint Progress toward multiplexed sample-to-result detection in low resource settings using microfluidic immunoassay cards
    Lisa Lafleur
    Department of Electrical Engineering, University of Washington, Seattle, WA, USA
    Lab Chip 12:1119-27. 2012
    ..All reagents were stored on card in dry form; only the sample and buffer were required to run the tests. Here we detail the development of this platform and discuss its strengths and weaknesses...
  21. doi request reprint Two-dimensional paper networks: programmable fluidic disconnects for multi-step processes in shaped paper
    Barry R Lutz
    Department of Bioengineering, University of Washington, Box 355061, Seattle, WA, USA
    Lab Chip 11:4274-8. 2011
    ..This approach could enable multi-step laboratory assays to be converted into simple point-of-care devices that have high performance yet remain easy to use...
  22. pmc Dynamic bioprocessing and microfluidic transport control with smart magnetic nanoparticles in laminar-flow devices
    James J Lai
    Department of Bioengineering, University of Washington, Seattle, WA 98195, USA
    Lab Chip 9:1997-2002. 2009
    ..This stimuli-responsive reagent system has been shown to transfer 81% of a model protein target from an input flowstream to a second flowstream in a continuous flow H-filter device...
  23. pmc Concentration gradient immunoassay. 2. Computational modeling for analysis and optimization
    Jennifer O Foley
    Department of Bioengineering, and Department of Chemical Engineering, University of Washington, Seattle, Washington 98195, USA
    Anal Chem 79:3549-53. 2007
    ..Given the experimental verification of the computational results, this model serves as an efficient method to explore the influence of the flow rate, microchannel dimensions, and antibody concentration on the sensitivity of the assay...
  24. pmc Concentration gradient immunoassay. 1. An immunoassay based on interdiffusion and surface binding in a microchannel
    Kjell E Nelson
    Department of Bioengineering, University of Washington, Seattle, Washington 98195, USA
    Anal Chem 79:3542-8. 2007
    ..This assay method is suitable for rapid quantitative detection of low molecular weight analytes for point-of-care diagnostic instrumentation...
  25. ncbi request reprint SPR imaging-based salivary diagnostics system for the detection of small molecule analytes
    Elain Fu
    Department of Bioengineering, University of Washington, Seattle, WA 98195, USA
    Ann N Y Acad Sci 1098:335-44. 2007
    ..The multicomponent system includes a hand-portable surface plasmon resonance (SPR) imaging instrument and a disposable microfluidic assay card...
  26. pmc Enhanced sensitivity of lateral flow tests using a two-dimensional paper network format
    Elain Fu
    Box 355061, Department of Bioengineering, University of Washington, Seattle, Washington, USA
    Anal Chem 83:7941-6. 2011
    ..These results highlight the potential of the paper network platform to enhance access to high-quality diagnostic capabilities in low-resource settings in the developed and developing worlds...
  27. ncbi request reprint High-throughput screening of enzyme inhibition using an inhibitor gradient generated in a microchannel
    Elena Garcia
    Department of Bioengineering, University of Washington, Box 355061, Foege N530J, 1705 NE Pacific Street, Seattle, WA 98195 2255, USA
    Lab Chip 7:249-55. 2007
    ..Recommendations for use of the assay are provided, including its use for high-throughput screening in drug discovery and general use in measurement of enzyme inhibition...
  28. pmc Dependence of the signal amplification potential of colloidal gold nanoparticles on resonance wavelength in surface plasmon resonance-based detection
    Elain Fu
    Department of Bioengineering, University of Washington, Seattle, WA 98195, United States
    Anal Chim Acta 599:118-23. 2007
    ..The results demonstrate an almost twofold signal difference for a subset of the experimental conditions explored...
  29. doi request reprint Rapid protein depletion from complex samples using a bead-based microfluidic device for the point of care
    Katherine G McKenzie
    Department of Bioengineering, University of Washington, Box 355061, Foege N530J, Seattle, WA 98195 5061, USA
    Lab Chip 9:3543-8. 2009
    ..This design can easily be adapted for depletion of other high abundance or interfering proteins by inclusion of other ligand immobilized beads...
  30. ncbi request reprint Passive electrophoresis in microchannels using liquid junction potentials
    Matthew S Munson
    Department of Bioengineering, University of Washington, Box 352141, Seattle, WA 98195, USA
    Electrophoresis 23:2642-52. 2002
    ..Possible practical microfluidic applications of electrophoretic transport without electrodes are discussed...
  31. ncbi request reprint Long-term dry storage of an enzyme-based reagent system for ELISA in point-of-care devices
    Sujatha Ramachandran
    University of Washington, Department of Bioengineering, Seattle, Washington, USA
    Analyst 139:1456-62. 2014
    ..These results demonstrate the potential of enzyme-based signal amplification for enhanced sensitivity in POC devices for low resource settings. ..
  32. pmc Imaging of surfaces by concurrent surface plasmon resonance and surface plasmon resonance-enhanced fluorescence
    Rahber Thariani
    Department of Bioengineering, University of Washington, Seattle, Washington, United States of America
    PLoS ONE 5:e9833. 2010
    ..The instrument allows for high performance imaging and quantitative measurements with surface plasmon resonance, and surface plasmon induced fluorescence, with inexpensive off-the-shelf components...
  33. doi request reprint Enabling a microfluidic immunoassay for the developing world by integration of on-card dry reagent storage
    Dean Y Stevens
    Department of Bioengineering, University of Washington, Box 355061, Foege N530J, 1705 NE Pacific Street, Seattle, WA 98195 5061, USA
    Lab Chip 8:2038-45. 2008
    ..The system gave a detection limit in the sub-nanomolar range in under nine minutes, showing the potential to expand into quantitative, multi-analyte analysis of human blood samples...
  34. pmc A practical guide to the staggered herringbone mixer
    Manda S Williams
    Department of Bioengineering, University of Washington, Seattle, WA 98195, USA
    Lab Chip 8:1121-9. 2008
    ..This analytical model is applicable to other solute systems, and possibly to other embodiments of the mixer, to enable optimal design, operation, and estimation of performance...
  35. ncbi request reprint Lateral spread of an amplification signal using an enzymatic system on a conductive surface
    Melissa S Hasenbank
    University of Washington, Department of Bioengineering, Box 355061, Seattle, Washington 98195, USA
    Langmuir 22:7451-3. 2006
    ....
  36. pmc Tunable-delay shunts for paper microfluidic devices
    Bhushan J Toley
    Department of Bioengineering, University of Washington, Seattle, Washington 98195, United States
    Anal Chem 85:11545-52. 2013
    ..Finally, the shunts were used for the sequential delivery of fluids to a detection zone in a point-of-care compatible folding card device using biochemical reagents for the amplified detection of the malaria protein PfHRP2. ..
  37. pmc Controlled reagent transport in disposable 2D paper networks
    Elain Fu
    Box 355061, Department of Bioengineering, University of Washington, Seattle, WA, USA
    Lab Chip 10:918-20. 2010
    ..Specifically, we demonstrate methods of controlling fluid transport using the geometry of the network and dissolvable barriers. Finally, we discuss the implications for higher sensitivity detection using this type of 2D paper network...
  38. ncbi request reprint Diffusion based analysis in a sheath flow microchannel: the sheath flow T-sensor
    Matthew S Munson
    University of Washington, Department of Bioengineering, Box 352255, Seattle, WA, USA
    Lab Chip 5:856-62. 2005
    ..Measurements for both a small molecule (rhodamine B) and an intermediate-sized protein (wheat germ agglutinin) were made, demonstrating the utility of the sheath flow T-sensor...
  39. pmc Highly sensitive immunoassay based on controlled rehydration of patterned reagents in a 2-dimensional paper network
    Gina E Fridley
    University of Washington, Department of Bioengineering, Box 355061, Seattle, Washington 98195, United States
    Anal Chem 86:6447-53. 2014
    ..In this demonstration, the signal enhancement step increases the visible signal by roughly 3-fold and decreases the analytical limit of detection by 2.75-fold. ..
  40. ncbi request reprint Suppression of non-specific adsorption using sheath flow
    Matthew S Munson
    University of Washington, Box 352141, Seattle, WA, USA
    Lab Chip 4:438-45. 2004
    ..The controlled adsorption of the protein bovine serum albumin (BSA) to a gold surface is also demonstrated using SPR microscopy...
  41. ncbi request reprint Recirculating flow accelerates DNA microarray hybridization in a microfluidic device
    Hyun Ho Lee
    Department of Bioengineering, University of Washington, Seattle, WA 98195, USA
    Lab Chip 6:1163-70. 2006
    ..54 degrees C, relative to static conditions. The experimental results were consistent with a three-dimensional simulation of hybridization using a recirculating buffer system...
  42. ncbi request reprint Interfacial instabilities affect microfluidic extraction of small molecules from non-Newtonian fluids
    Kristen L Helton
    Department of Bioengineering, University of Washington, 1705 NE Pacific Street, Seattle, WA 98195, USA
    Lab Chip 7:1581-8. 2007
    ....
  43. ncbi request reprint Controlled microfluidic reconstitution of functional protein from an anhydrous storage depot
    Elena Garcia
    Department of Bioengineering, University of Washington, Box 352141, Seattle, WA 98195 2255, USA
    Lab Chip 4:78-82. 2004
    ..More broadly, this preservation and controlled release technique can be applied where the preservation and/or spatial and temporal control of chemical concentrations are desired...
  44. ncbi request reprint A smart microfluidic affinity chromatography matrix composed of poly(N-isopropylacrylamide)-coated beads
    Noah Malmstadt
    Department of Bioengineering, University of Washington, Seattle, Washington 98195, USA
    Anal Chem 75:2943-9. 2003
    ..This temperature-responsive affinity chromatography matrix can thus be flowed into a column and aggregated via temperature change, followed by the controlled release of affinity-captured targets back into the microfluidic flow stream...
  45. ncbi request reprint Cell lysis and protein extraction in a microfluidic device with detection by a fluorogenic enzyme assay
    Eric A Schilling
    Department of Bioengineering, University of Washington, Seattle 98195, USA
    Anal Chem 74:1798-804. 2002
    ..While the focus is on the lysis of typical gram-negative bacterial cells (E. coli), the techniques described here could, in principle, be applied to a variety of different cell types...
  46. ncbi request reprint Cross-talk problem on a fluorescence multi-channel microfluidic chip system
    Rudi Irawan
    Biomedical Engineering Research Centre, School of Electrical and Electronic Engineering, Nanyang Technological University, Singapore 637553
    Biomed Microdevices 7:205-11. 2005
    ..We monitored this cross-talk using fluorescein as a fluorescent sample and Mylar sheeting as a microfluidic chip material. We then discuss how this cross-talk can be avoided using a simple, inexpensive and effective method...
  47. pmc Compact, high performance surface plasmon resonance imaging system
    Timothy M Chinowsky
    Biosens Bioelectron 22:2208-15. 2007
    ..The simple, compact construction and high performance of the imager will allow the device to be readily applied to a wide range of applications...

Research Grants5

  1. A Microfluidic Diffusion Immunoassay
    Paul Yager; Fiscal Year: 2001
    ..abstract_text> ..
  2. A Microfluidic Diffusion Immunoassay
    Paul Yager; Fiscal Year: 2002
    ..abstract_text> ..
  3. A Microfluidic Diffusion Immunoassay
    Paul Yager; Fiscal Year: 2003
    ..abstract_text> ..
  4. A Microfluidic Diffusion Immunoassay
    Paul Yager; Fiscal Year: 2004
    ..abstract_text> ..
  5. Rapid parallel salivary immunoassays on a disposable
    Paul Yager; Fiscal Year: 2005
    ..Finally, a versatile combined system will be designed and tested that will allow monitoring of samples by the two methods simultaneously. ..