Affiliation: University of Massachusetts Medical School
Yin H, Xue W, Anderson D. CRISPR-Cas: a tool for cancer research and therapeutics. Nat Rev Clin Oncol. 2019;: pubmed publisher
..In this Review, we discuss these applications as well as new potential uses of CRISPR to assist in cancer detection or the development of anticancer therapies. ..
Smith J, Mou H, Xue W. Understanding and repurposing CRISPR-mediated alternative splicing. Genome Biol. 2018;19:184 pubmed publisher
..Two new studies refine our understanding of CRISPR-associated exon skipping and redefine its utility in engineering alternative splicing. ..
Li Y, Park A, Mou H, Colpan C, Bizhanova A, Akama Garren E, et al
. A versatile reporter system for CRISPR-mediated chromosomal rearrangements. Genome Biol. 2015;16:111 pubmed publisher
..Moreover, we detect Cas9 cleavage at the fourth nucleotide on the non-complementary strand, leading to staggered instead of blunt DNA breaks. These reporters allow mechanisms of chromosomal rearrangements to be investigated. ..
Sheel A, Xue W. Genomic Amplifications Cause False Positives in CRISPR Screens. Cancer Discov. 2016;6:824-6 pubmed publisher
..Cancer Discov; 6(8); 824-6. ©2016 AACR.See related article by Munoz et al., p. 900See related article by Aguirre et al., p. 914. ..
Song C, Li Y, Mou H, Moore J, Park A, Pomyen Y, et al
. Genome-Wide CRISPR Screen Identifies Regulators of Mitogen-Activated Protein Kinase as Suppressors of Liver Tumors in Mice. Gastroenterology. 2017;152:1161-1173.e1 pubmed publisher
..We associated decreased levels of NF1 and increased levels of its downstream protein HMGA2 with survival times of patients with HCC. Strategies to inhibit or reduce HMGA2 might be developed to treat patients with liver cancer. ..
Mou H, Smith J, Peng L, Yin H, Moore J, Zhang X, et al
. CRISPR/Cas9-mediated genome editing induces exon skipping by alternative splicing or exon deletion. Genome Biol. 2017;18:108 pubmed publisher
..Exon skipping adds to the unexpected outcomes that must be accounted for, and perhaps taken advantage of, in CRISPR experiments. ..