Research Topics
Genomes and Genes | TODD WYATTSummaryAffiliation: University of Nebraska Medical Center Country: USA Publications
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Publications
Non-typeable Haemophilus influenzae decreases cilia beating via protein kinase C epsilonKRISTINA L BAILEY
Department of Epidemiology, College of Public Health, University of Nebraska Medical Center, Omaha, NE 68198 5910, USA
Respir Res 13:49. 2012..The subsequent auto-downregulation of PKCε or the direct inhibition of PKCε leads to the specific detachment of the ciliated cells...- Co-exposure to cigarette smoke and alcohol decreases airway epithelial cell cilia beating in a protein kinase Cε-dependent mannerTodd A Wyatt
VA Nebraska Western Iowa Health Care System Research Service, Department of Veterans Affairs Medical Center, Omaha, Nebraska, USA
Am J Pathol 181:431-40. 2012..No decreases in CBF were observed in PKCε knockout mice co-exposed to smoke and alcohol. These data identify PKCε as a key regulator of cilia slowing in response to combined smoke and alcohol-induced lung injury... - Malondialdehyde-acetaldehyde-adducted protein inhalation causes lung injuryTodd A Wyatt
Research Service, Veteran Affairs Nebraska Western Iowa Health Care System, Omaha, NE 68105, USA
Alcohol 46:51-9. 2012..These data further implicate MAA-adduct formation as a potential mechanism for smoke- and alcohol-induced lung injury... 
Malondialdehyde-acetaldehyde adducts decrease bronchial epithelial wound repairTodd A Wyatt
Research Service, Department of Veterans Affairs Medical Center, 4101 Woolworth Avenue, Omaha, NE 68105, USA
Alcohol 36:31-40. 2005..Scavenger receptor-mediated activation of PKC alpha may function to reduce wound healing under conditions of alcohol and cigarette smoke exposure where malondialdehyde-acetaldehyde adducts may be present...- Both cAMP and cGMP are required for maximal ciliary beat stimulation in a cell-free model of bovine ciliary axonemesTodd A Wyatt
Research Service, Department of Veterans Affairs Medical Center, Omaha, Nebraska, USA
Am J Physiol Lung Cell Mol Physiol 288:L546-51. 2005..However, the cyclic nucleotides did not stimulate CBF in the absence of ATP. Therefore, the combination of cAMP and cGMP augments ATP-driven CBF increases at the level of isolated axoneme... - Desensitization of PKA-stimulated ciliary beat frequency in an ethanol-fed rat model of cigarette smoke exposureTodd A Wyatt
Department of Internal Medicine, Pulmonary, Critical Care, and Sleep Medicine Section, University of Nebraska Medical Center, Omaha, Nebraska 68198 5300, USA
Alcohol Clin Exp Res 28:998-1004. 2004..We hypothesized that a similar ethanol-mediated desensitization of CBF would occur in vivo... - Sequential activation of protein kinase C isoforms by organic dust is mediated by tumor necrosis factorTodd A Wyatt
Department of Internal Medicine, Pulmonary, Critical Care, Sleep and Allergy Section, University of Nebraska Medical Center, 985300 Nebraska Medical Center, Omaha, NE 68198 5300, USA
Am J Respir Cell Mol Biol 42:706-15. 2010..These results suggest a temporal ordering of events responsible for the release of cytokines, which initiate and exacerbate inflammatory events in the airways of people exposed to agricultural dust... - Ethanol stimulates ciliary beating by dual cyclic nucleotide kinase activation in bovine bronchial epithelial cellsTodd A Wyatt
Research Service, Department of Veterans Affairs Medical Center, Omaha, Nebraska 68198, USA
Am J Pathol 163:1157-66. 2003..8-pCPT-cGMPS, an antagonist analogue to cGMP, blocked EtOH-stimulated PKA activity increases. These data support that EtOH-stimulated increases in CBF require the dual activation of both PKA (via cAMP) and PKG (via NO)... - Protein kinase C activation is required for cigarette smoke-enhanced C5a-mediated release of interleukin-8 in human bronchial epithelial cellsT A Wyatt
Research Service, Department of Veterans Affairs Medical Center, Omaha, Nebraska, USA
Am J Respir Cell Mol Biol 21:283-8. 1999..These results suggest that PKC activation by CSE alone does not result in IL-8 release, but that CSE-stimulated PKC activation is required for C5a-mediated IL-8 release from HBECs... - Relaxin stimulates bronchial epithelial cell PKA activation, migration, and ciliary beatingT A Wyatt
Research Service, Department of Veterans Affairs Medical Center, Omaha, NE 68105, USA
Exp Biol Med (Maywood) 227:1047-53. 2002..These data suggest that relaxin augments epithelial repair by increasing airway cell migration and CBF via PKA-dependent mechanisms... - Exposure to hog barn dust alters airway epithelial ciliary beatingT A Wyatt
Dept of Internal Medicine, Pulmonary, Critical Care, Sleep and Allergy Section, University of Nebraska Medical Center, Omaha, NE 68198 5300, USA
Eur Respir J 31:1249-55. 2008..These data demonstrate that hog barn dust can alter normal stimulation of cilia, suggesting a mechanism for the abrogation of stimulated increases in mucociliary clearance in response to inhaled dust exposure... - Malondialdehyde-acetaldehyde-adducted bovine serum albumin activates protein kinase C and stimulates interleukin-8 release in bovine bronchial epithelial cellsT A Wyatt
Research Service, Department of Veterans Affairs Medical Center, 4101 Woolworth Avenue, Omaha, NE 68105, USA
Alcohol 25:159-66. 2001.... - Chronic ethanol downregulates PKA activation and ciliary beating in bovine bronchial epithelial cellsT A Wyatt
Research Service, Department of Veterans Affairs Medical Center, Omaha, Nebraska 68105, USA
Am J Physiol Lung Cell Mol Physiol 281:L575-81. 2001..EtOH desensitizes BBEC to PKA-activating agents, suggesting that EtOH rapidly stimulates, whereas long-term EtOH downregulates, CBF via PKA in BBEC... - Acetaldehyde-stimulated PKC activity in airway epithelial cells treated with smoke extract from normal and smokeless cigarettesT A Wyatt
Veterans Affairs Medical Center Research Services and Pulmonary and Critical Care Medicine, Department of Internal Medicine, University of Nebraska Medical Center, Omaha, Nebraska 68198 5300, USA
Proc Soc Exp Biol Med 225:91-7. 2000..These results suggest that volatile components such as acetaldehyde in cigarette smoke may inhibit ciliary motility via a PKC-dependent mechanism... - Effects of cigarette smoke and alcohol on ciliated tracheal epithelium and inflammatory cell recruitmentMargaret K Elliott
University of Nebraska Medical Center, 985300 Nebraska Medical Center, Omaha, NE 68198 5300, USA
Am J Respir Cell Mol Biol 36:452-9. 2007.... - Nitric oxide-dependent cilia regulatory enzyme localization in bovine bronchial epithelial cellsSarah L Stout
Pulmonary, Critical Care, Sleep and Allergy Medicine, Dept of Internal Medicine, University of Nebraska Medical Center 985300, Omaha, NE 68198 5300, USA
J Histochem Cytochem 55:433-42. 2007..Our findings suggest that these enzymes are sequestered close to their points of action into a discrete ciliary metabolon, enabling targeted phosphorylation and efficient upregulation of ciliary beating... - Inhibition of protein kinase C epsilon causes ciliated bovine bronchial cell detachmentRebecca E Slager
Pulmonary, Critical Care and Sleep Medicine Section, Department of Internal Medicine, University of Nebraska Medical Center, Omaha, Nebraska 68198-5300, USA
Exp Lung Res 32:349-62. 2006..The results of this study support a possible novel PKCepsilon-mediated signaling pathway through which ciliated cell attachment is maintained... - Feedlot dust stimulation of interleukin-6 and -8 requires protein kinase Cepsilon in human bronchial epithelial cellsTodd A Wyatt
Pulmonary and Critical Care Medicine Section, Department of Internal Medicine, Omaha, NE, USA
Am J Physiol Lung Cell Mol Physiol 293:L1163-70. 2007..These data support the hypothesis that FLDE exposure stimulates bronchial epithelial IL-8 and IL-6 release via a PKCepsilon-dependent pathway... - Smoke exposure exacerbates an ethanol-induced defect in mucociliary clearance of Streptococcus pneumoniaeElizabeth A Vander Top
Veterans Affairs Medical Center, Omaha, Nebraska 68105, USA
Alcohol Clin Exp Res 29:882-7. 2005..Defects in mucociliary clearance thus may be one cause of the increased risk of pneumococcal infections in people who abuse alcohol, particularly if they also smoke... - Hog barn dust slows airway epithelial cell migration in vitro through a PKCalpha-dependent mechanismRebecca E Slager
Department of Internal Medicine, Pulmonary and Critical Care Medicine Section, University of Nebraska Medical Center, Omaha, NE 68198 5300, USA
Am J Physiol Lung Cell Mol Physiol 293:L1469-74. 2007..These results suggest that the PKCalpha isoform is an important regulator of cell migration in response to agricultural dust exposure... - Ethanol increases phosphodiesterase 4 activity in bovine bronchial epithelial cellsMary A Forgèt
Pulmonary and Critical Care Medicine Section, Department of Internal Medicine, University of Nebraska Medical Center, 985300 Nebraska Medical Center, Omaha, NE 68198 5300, USA
Alcohol 31:31-8. 2003..Other compensatory mechanisms however, may be responsible for the down-regulation of PKA, which occurs after chronic epithelial exposure (>/=6 h) to ethanol... - Alcohol functionally upregulates Toll-like receptor 2 in airway epithelial cellsKRISTINA L BAILEY
Department of Internal Medicine, Pulmonary, Critical Care, Sleep and Allergy Section, University of Nebraska Medical Center, Omaha, Nebraska 68198 5815, USA
Alcohol Clin Exp Res 33:499-504. 2009..Little is known about why this phenomenon is observed. We hypothesized that alcohol may modulate Toll-like receptor 2 (TLR2), which regulates inflammation caused by gram-positive bacteria... - Differential in vivo effects of whole cigarette smoke exposure versus cigarette smoke extract on mouse ciliated tracheal epitheliumMargaret K Elliott
Pulmonary, Critical Care and Sleep Medicine Section, University of Nebraska Medical Center, Omaha, Nebraska 68198-5300, USA
Exp Lung Res 32:99-118. 2006..CSE exposure induced inflammatory cell bronchiolitis. These data indicate that CSE exposure has differential effects on the lungs and tracheal epithelium compared to CS exposure... - Protein kinase C-alpha mediates cigarette smoke extract- and complement factor 5a-stimulated interleukin-8 release in human bronchial epithelial cellsRavindra Kashyap
Pulmonary and Critical Care Medicine Section, Department of Internal Medicine, University of Nebraska Medical Center, Omaha 68198-5300, USA
J Investig Med 50:46-53. 2002..CONCLUSIONS: Collectively, these data suggest that PKC activators in addition to CSE augment C5a-stimulated IL-8 release from HBEC and that CSE and C5a stimulate IL-8 release in HBEC by activating the calcium-dependent PKCalpha isoform... - Ethanol attenuates contraction of primary cultured rat airway smooth muscle cellsPeter J Oldenburg
Department of Internal Medicine, Pulmonary, Critical Care, Sleep, and Allergy Division, University of Nebraska Medical Center, Omaha, Nebraska 68198 5910, USA
Am J Respir Cell Mol Biol 43:539-45. 2010..In addition, it appears that this effect is the result of activation of the cGMP/PKG kinase pathway. These findings implicate a direct effect of ethanol on airway smooth muscle cells as the basis for in vivo ethanol effects... - Inhibition of human airway smooth muscle cell proliferation by beta 2-adrenergic receptors and cAMP is PKA independent: evidence for EPAC involvementKaren M Kassel
Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, NE 68198 5800, USA
Am J Physiol Lung Cell Mol Physiol 294:L131-8. 2008.... - Adenosine promotion of cellular migration in bronchial epithelial cells is mediated by the activation of cyclic adenosine monophosphate-dependent protein kinase ADiane S Allen-Gipson
Department of Internal Medicine, Nebraska Medical Center, Omaha, NE 68198 5815, USA
J Investig Med 55:378-85. 2007..These data suggest that adenosine activation of A(2A)AR augments epithelial repair by increasing airway cellular migration by PKA-dependent mechanisms... 
Alcohol up-regulates TLR2 through a NO/cGMP dependent pathwayKRISTINA L BAILEY
Pulmonary, Critical Care, Sleep and Allergy Section, Department of Internal Medicine, University of Nebraska Medical Center, Omaha, Nebraska, USA
Alcohol Clin Exp Res 34:51-6. 2010..We have previously shown that alcohol up-regulates TLR2 in the airway epithelium. However, the mechanism of alcohol-mediated up-regulation of TLR2 has not been identified...- Alcohol stimulates ciliary motility of isolated airway axonemes through a nitric oxide, cyclase, and cyclic nucleotide-dependent kinase mechanismJoseph H Sisson
Department of Internal Medicine, University of Nebraska Medical Center, Pulmonary, Critical Care, Sleep and Allergy Section, Omaha, Nebraska 68198 5300, USA
Alcohol Clin Exp Res 33:610-6. 2009.... - Ethanol blocks adenosine uptake via inhibiting the nucleoside transport system in bronchial epithelial cellsDiane S Allen-Gipson
Department of Internal Medicine, Pulmonary, Critical Care, Sleep and Allergy Section, 985815 Nebraska Medical Center, Omaha, NE 68198 5815, USA
Alcohol Clin Exp Res 33:791-8. 2009..Taken together, we hypothesized that ethanol impairs adenosine uptake via the nucleoside transport system... - Intranasal organic dust exposure-induced airway adaptation response marked by persistent lung inflammation and pathology in miceJill A Poole
Pulmonary, Critical Care, Sleep, and Allergy Section, Univ of Nebraska Medical Center, 985300 The Nebraska Medical Center, Omaha, NE 68198 5300, USA
Am J Physiol Lung Cell Mol Physiol 296:L1085-95. 2009..Collectively, intranasal exposure to DE results in significant lung inflammatory and pathological responses marked by a modulated innate immune response to single and repetitive dust exposures that is associated with PKC activity... - Association of chronic alcohol consumption and increased susceptibility to and pathogenic effects of pulmonary infection with respiratory syncytial virus in miceThomas R Jerrells
Department of Pathology and Microbiology, University of Nebraska Medical Center, 986495 Nebraska Medical Center, Omaha, Nebraska 68198 6495, USA
Alcohol 41:357-69. 2007..Infection of mice that had consumed alcohol chronically was more severe in terms of increased proinflammatory cytokine production, inflammation, and a failure to clear the virus from the lungs... - Effect of malondialdehyde-acetaldehyde-protein adducts on the protein kinase C-dependent secretion of urokinase-type plasminogen activator in hepatic stellate cellsKusum K Kharbanda
Department of Veterans Affairs Medical Center, VA Alcohol Research Center, 4101 Woolworth Avenue, Omaha, NE 68105, USA
Biochem Pharmacol 63:553-62. 2002..These results indicate that MAA adducts via a PKC-mediated pathway may regulate plasmin-mediated matrix degradation in the liver, thereby contributing to the progression of hepatic fibrosis... - Activation of protein kinase A accelerates bovine bronchial epithelial cell migrationJohn R Spurzem
Department of Veterans Affairs Medical Center, Omaha, Nebraska 68105, USA
Am J Physiol Lung Cell Mol Physiol 282:L1108-16. 2002..Augmentation of PKA activity reduced the levels of active Rho and the formation of focal adhesions. These studies suggest that PKA activation modulates Rho activity, migration mechanisms, and thus bronchial epithelial repair mechanisms... - Smoke and C5a induce airway epithelial intercellular adhesion molecule-1 and cell adhesionAnthony A Floreani
Division of Pulmonary and Critical Care Medicine, School of Allied Health Professions, University of Nebraska Medical Center Nebraska Health Systems, Omaha, NE 68198 5300, USA
Am J Respir Cell Mol Biol 29:472-82. 2003..Our results may help explain the initiation and propagation of inflammatory events in vivo induced by chronic airway exposure to cigarette smoke... - Transforming growth factor-beta induces contraction of activated hepatic stellate cellsKusum K Kharbanda
Department of Veterans Affairs Medical Center, VA Alcohol Research Center, 4101 Woolworth Avenue, Omaha, NE 68105, USA
J Hepatol 41:60-6. 2004..Recognizing the prominent roles that hepatic stellate cells (HSCs) and TGF-beta play in portal hypertension and fibrogenesis, respectively, we sought to evaluate the effect of TGF-beta on the contractility of activated HSCs... - Ethanol treatment reduces bovine bronchial epithelial cell migrationJohn R Spurzem
Department of Internal Medicine, Omaha Veterans Affairs Medical Center, Omaha, Nebraska 68198 5300, USA
Alcohol Clin Exp Res 29:485-92. 2005..We hypothesize that chronic ethanol exposure down-regulates the adenosine 3':5'-cyclic monophosphate signaling cascade in airway epithelial cells, resulting in decreased epithelial cell migration and repair... - Cigarette smoke extract increases C5a receptor expression in human bronchial epithelial cellsDiane S Allen-Gipson
Pulmonary, Critical Care, and Sleep Medicine Section, Department of Internal Medicine, University of Nebraska Medical Center, 985815 Nebraska Medical Center, Omaha, NE 68198 5815, USA
J Pharmacol Exp Ther 314:476-82. 2005..The data also indicate that PKC-alpha may play an important role in modulating C5aR binding... - Alcohol intake is associated with altered pulmonary functionJoseph H Sisson
Pulmonary and Critical Medicine Section, Internal Medicine Department, University of Nebraska Medical Center, Omaha, NE 68198 5300, USA
Alcohol 36:19-30. 2005..Our analyses indicate that alcohol consumption, even at very modest intake levels, is associated with less lung restriction... - RACK1, a PKC targeting protein, is exclusively localized to basal airway epithelial cellsRebecca E Slager
Department of Internal Medicine, Pulmonary and Critical Care Medicine Section, University of Nebraska Medical Center, 985300 Nebraska Medical Center, Omaha, NE 68198 5300, USA
J Histochem Cytochem 56:7-14. 2008..These results suggest that PKCepsilon signaling in the basal airway cell may involve RACK1; however, PKCepsilon regulation in ciliated cells uses RACK1-independent pathways... - Repeat organic dust exposure-induced monocyte inflammation is associated with protein kinase C activityJill A Poole
Pulmonary, Critical Care, Sleep and Allergy Section, Department of Internal Medicine, University of Nebraska Medical Center, 985300 The Nebraska Medical Center, Omaha, NE 68198 5300, USA
J Allergy Clin Immunol 120:366-73. 2007..Organic dust exposure results in an inflammatory response that attenuates over time, but repetitive exposures can result in chronic respiratory diseases. Mechanisms underlying this modulated response are not clear... - Prostaglandin F2alpha represses IGF-I-stimulated IRS1/phosphatidylinositol-3-kinase/AKT signaling in the corpus luteum: role of ERK and P70 ribosomal S6 kinaseEdward Arvisais
Omaha Veterans Affairs Medical Center, Omaha, Nebraska 68105, USA
Mol Endocrinol 24:632-43. 2010..It seems likely that the rapid changes in phosphorylation events are among the early events that mediate PGF2alpha-induced corpus luteum regression... - Direct involvement of the isotype-specific C-terminus of beta tubulin in ciliary beatingJulia Vent
Department of Biomedical Sciences, Creighton University, Omaha, Nebraska 68178, USA
J Cell Sci 118:4333-41. 2005.... - Convergence of 3',5'-cyclic adenosine 5'-monophosphate/protein kinase A and glycogen synthase kinase-3beta/beta-catenin signaling in corpus luteum progesterone synthesisLynn Roy
Department of Obstetrics and Gynecology, Olson Center for Women s Health, 3255 Nebraska Medical Center, Omaha, Nebraska 68198, USA
Endocrinology 150:5036-45. 2009..Collectively these data suggest that cAMP/PKA regulation of GSK3beta/beta-catenin signaling may contribute to the acute increase in progesterone production in response to LH... - Bronchodilator responsiveness in swine veterinariansJill A Poole
Department of Medicine, University of Nebraska Medical Center, Omaha 68198 5300, USA
J Agromedicine 12:49-54. 2007..The present study reassessed the prevalence of respiratory complaints and pulmonary function abnormalities in swine veterinarians and sought to characterize their response to bronchodilators... - Long-term cigarette smoke exposure in a mouse model of ciliated epithelial cell functionSamantha M Simet
Pulmonary, Critical Care, Sleep, and Allergy Division, Department of Internal Medicine, University of Nebraska Medical Center, 985910 Nebraska Medical Center, Omaha, NE 68198 5910, USA
Am J Respir Cell Mol Biol 43:635-40. 2010..In parallel with the slowing of CBF, significant PKC activation from cytosol to the membrane of tracheal epithelial cells was detected in mice exposed to smoke for 6-12 months... - Tumor necrosis factor-alpha hyper-responsiveness to endotoxin in whole blood is associated with chronic bronchitis in farmersG Patrick Lambert
Department of Exercise Science and Athletic Training, Creighton University, Omaha, NE 68178, USA
J Agromedicine 10:39-44. 2005.... - Exercise training during diabetes attenuates cardiac ryanodine receptor dysregulationChun Hong Shao
Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, NE, USA
J Appl Physiol 106:1280-92. 2009..These data are the first to show that ExT during diabetes normalizes RyR2 function and Ca(2+) release from the sarcoplasmic reticulum, providing insights into mechanisms by which ExT during diabetes improves cardiac function... - PKCdelta mediates thrombin-augmented fibroblast-mediated collagen gel contractionQiuhong Fang
Beijing Shijitan Hospital, Peking University, Beijing, China
Biochem Biophys Res Commun 369:1199-203. 2008..These results suggest that thrombin contributes to the tissue remodeling in inflammatory airways and lung diseases at least partially through both PKCdelta and PKCepsilon signaling... - PGD(2) modulates fibroblast-mediated native collagen gel contractionTadashi Kohyama
Department of Respiratory Medicine, University of Tokyo, Tokyo, Japan
Am J Respir Cell Mol Biol 27:375-81. 2002.... - Maternal alcohol ingestion reduces surfactant protein A expression by preterm fetal lung epitheliaTatjana Lazic
Department of Veterinary Pathology, 2740 College of Veterinary Medicine, Iowa State University, Ames, IA 50011 1250, USA
Alcohol 41:347-55. 2007..These alterations may underlie increased susceptibility of preterm infants, exposed to ethanol in utero, to RSV and other microbial agents... - Primary ciliary dyskinesia in mice lacking the novel ciliary protein Pcdp1Lance Lee
Department of Pathology, Enders 1116, Children s Hospital Boston, 300 Longwood Avenue, Boston, MA 02115, USA
Mol Cell Biol 28:949-57. 2008..This study demonstrates that Pcdp1 plays an important role in ciliary and flagellar biogenesis and motility, making the nm1054 mutant a useful model for studying the molecular genetics and pathogenesis of PCD...
Research Grants
- Airway Injury Caused by MAA AdductsTodd A Wyatt; Fiscal Year: 2010....
- Airway Injury Caused by MAA AdductsTODD WYATT; Fiscal Year: 2009....