Affiliation: University of Wisconsin
Wuthrich M, Hung C, Gern B, Pick Jacobs J, Galles K, Filutowicz H, et al
. A TCR transgenic mouse reactive with multiple systemic dimorphic fungi. J Immunol. 2011;187:1421-31 pubmed publisher
Wuthrich M, Ersland K, Pick Jacobs J, Gern B, Frye C, Sullivan T, et al
. Limited model antigen expression by transgenic fungi induces disparate fates during differentiation of adoptively transferred T cell receptor transgenic CD4+ T cells: robust activation and proliferation with weak effector function during recall. Infect Immun. 2012;80:787-97 pubmed publisher
..However, the limited availability of model Ag expressed by Tg fungi during T cell priming blunts the downstream generation of effector and memory T cells. ..
Wuthrich M, Warner T, Klein B. IL-12 is required for induction but not maintenance of protective, memory responses to Blastomyces dermatitidis: implications for vaccine development in immune-deficient hosts. J Immunol. 2005;175:5288-97 pubmed
..In conclusion, these studies demonstrate that once CD4 and CD8 cells have acquired a protective T1 phenotype they no longer require the presence of IL-12 to maintain antifungal protective memory. ..
Wuthrich M, Warner T, Klein B. CD28 is required for optimal induction, but not maintenance, of vaccine-induced immunity to Blastomyces dermatitidis. Infect Immun. 2005;73:7436-41 pubmed
..Our findings suggest that CD28 is required for the induction of optimal, protective T-cell responses to B. dermatitidis infection but may be dispensable for the maintenance of T-cell memory...
Wuthrich M, Fisette P, Filutowicz H, Klein B. Differential requirements of T cell subsets for CD40 costimulation in immunity to Blastomyces dermatitidis. J Immunol. 2006;176:5538-47 pubmed
..Despite the plasticity of T cell subsets in vaccine immunity, in absence of CD40/CD40L interaction, CD4(+) cells may impede the priming of CD8(+) cells at the cost of host survival against a lethal infectious disease. ..
Wuthrich M, Finkel Jimenez B, Brandhorst T, Filutowicz H, Warner T, Klein B. Analysis of non-adhesive pathogenic mechanisms of BAD1 on Blastomyces dermatitidis. Med Mycol. 2006;44:41-9 pubmed
..dermatitidis by also deviating host adaptive immunity, and leukocyte responses...
Wuthrich M, Filutowicz H, Allen H, Deepe G, Klein B. V beta1+ J beta1.1+/V alpha2+ J alpha49+ CD4+ T cells mediate resistance against infection with Blastomyces dermatitidis. Infect Immun. 2007;75:193-200 pubmed
..Thus, the outgrowth of protective T-cell clones against immunodominant antigens of B. dermatitidis is biased by a combination of the TCR repertoire and Th1 cytokine production...
Wuthrich M, Gern B, Hung C, Ersland K, Rocco N, Pick Jacobs J, et al
. Vaccine-induced protection against 3 systemic mycoses endemic to North America requires Th17 cells in mice. J Clin Invest. 2011;121:554-68 pubmed publisher
..These data suggest that human vaccines against systemic fungal infections should be designed to induce Th17 cells if they are to be effective. ..
Wuthrich M, Krajaejun T, Shearn Bochsler V, Bass C, Filutowicz H, Legendre A, et al
. Safety, tolerability, and immunogenicity of a recombinant, genetically engineered, live-attenuated vaccine against canine blastomycosis. Clin Vaccine Immunol. 2011;18:783-9 pubmed publisher
..Thus, the live-attenuated vaccine against blastomycosis studied here proved safe, well tolerated, and immunogenic in dogs and merits further studies of vaccine efficacy...