Yi I Wu

Summary

Affiliation: University of North Carolina
Country: USA

Publications

  1. pmc A genetically encoded photoactivatable Rac controls the motility of living cells
    Yi I Wu
    Department of Pharmacology, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    Nature 461:104-8. 2009
  2. pmc Redesign of the PAK1 autoinhibitory domain for enhanced stability and affinity in biosensor applications
    Ramesh K Jha
    Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, NC 27599 7260, USA
    J Mol Biol 413:513-22. 2011
  3. ncbi request reprint Identification of novel integrin binding partners for calcium and integrin binding protein 1 (CIB1): structural and thermodynamic basis of CIB1 promiscuity
    Thomas C Freeman
    Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, and McAllister Heart Institute, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, United States
    Biochemistry 52:7082-90. 2013
  4. pmc Spatiotemporal control of small GTPases with light using the LOV domain
    Yi I Wu
    Department of Pharmacology and Lineberger Cancer Center, University of North Carolina, Chapel Hill, North Carolina, USA
    Methods Enzymol 497:393-407. 2011

Collaborators

  • Klaus M Hahn
  • Nikolay V Dokholyan
  • Thomas C Freeman
  • Ramesh K Jha
  • Justin L Black
  • Tina M Leisner
  • Ashutosh Tripathy
  • Onur Dagliyan
  • Holly G Bray
  • Leslie V Parise
  • Jon S Zawistowski
  • Brian Kuhlman
  • Chris Macnevin

Detail Information

Publications4

  1. pmc A genetically encoded photoactivatable Rac controls the motility of living cells
    Yi I Wu
    Department of Pharmacology, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    Nature 461:104-8. 2009
    ..A PA-Rac crystal structure and modelling revealed LOV-Rac interactions that will facilitate extension of this photoactivation approach to other proteins...
  2. pmc Redesign of the PAK1 autoinhibitory domain for enhanced stability and affinity in biosensor applications
    Ramesh K Jha
    Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, NC 27599 7260, USA
    J Mol Biol 413:513-22. 2011
    ..3 μM) than to full-length PAK1 in the "closed" state (undetectable affinity), and binding can be monitored with fluorescence by placing an environmentally sensitive fluorescence dye on CFP-PAcKer adjacent to the binding site...
  3. ncbi request reprint Identification of novel integrin binding partners for calcium and integrin binding protein 1 (CIB1): structural and thermodynamic basis of CIB1 promiscuity
    Thomas C Freeman
    Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, and McAllister Heart Institute, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, United States
    Biochemistry 52:7082-90. 2013
    ..These new mechanistic details of CIB1-integrin binding imply that CIB1 could bind to all integrin complexes and act as a broad regulator of integrin function. ..
  4. pmc Spatiotemporal control of small GTPases with light using the LOV domain
    Yi I Wu
    Department of Pharmacology and Lineberger Cancer Center, University of North Carolina, Chapel Hill, North Carolina, USA
    Methods Enzymol 497:393-407. 2011
    ..Here, we describe optimization and application of PA-Rac using detailed examples that we hope will help others apply the approach to different proteins and in a variety of different cells, tissues, and organisms...