Yang Wu

Summary

Affiliation: University of New Mexico
Country: USA

Publications

  1. pmc Discovery of regulators of receptor internalization with high-throughput flow cytometry
    Yang Wu
    Department of Pathology, School of Medicine, University of New Mexico, MSC08 4640, 700 Camino de Salud NE, IDTC Rm 2340, Albuquerque, NM 87131, USA
    Mol Pharmacol 82:645-57. 2012
  2. pmc High-throughput flow cytometry compatible biosensor based on fluorogen activating protein technology
    Yang Wu
    UNM Center for Molecular Discovery, University of New Mexico School of Medicine, Albuquerque, New Mexico 87131, USA
    Cytometry A 83:220-6. 2013
  3. pmc Rapid-mix flow cytometry measurements of subsecond regulation of G protein-coupled receptor ternary complex dynamics by guanine nucleotides
    Yang Wu
    Department of Pathology and Cancer Research Facility, University of New Mexico Health Sciences Center, University of New Mexico, Albuquerque, NM 87131, USA
    Anal Biochem 371:10-20. 2007
  4. pmc The development of quantum dot calibration beads and quantitative multicolor bioassays in flow cytometry and microscopy
    Yang Wu
    Department of Pathology and Cancer Center, University of New Mexico, Albuquerque, NM 87131, USA
    Anal Biochem 364:180-92. 2007
  5. pmc Spectroscopic characterization of streptavidin functionalized quantum dots
    Yang Wu
    Department of Pathology and Cancer Center, University of New Mexico School of Medicine, Albuquerque, NM 87131, USA
    Anal Biochem 364:193-203. 2007
  6. ncbi request reprint Small-volume rapid-mix device for subsecond kinetic analysis in flow cytometry
    Yang Wu
    Department of Chemical and Nuclear Engineering, University of New Mexico Health Sciences Center, Albuquerque, New Mexico 87131, USA
    Cytometry A 67:37-44. 2005
  7. doi request reprint Quantum dots for quantitative flow cytometry
    Tione Buranda
    Department of Pathology and Cancer Center, University of New Mexico School of Medicine, Albuquerque, NM, USA
    Methods Mol Biol 699:67-84. 2011
  8. pmc Identification of a small GTPase inhibitor using a high-throughput flow cytometry bead-based multiplex assay
    Zurab Surviladze
    University of New Mexico Center for Molecular Discovery, Albuquerque, NM 87131, USA
    J Biomol Screen 15:10-20. 2010
  9. doi request reprint Chapter 11. Subsecond analyses of G-protein coupled-receptor ternary complex dynamics by rapid mix flow cytometry
    Tione Buranda
    Department of Pathology and Cancer Center, University of New Mexico Health Science Center, Albuquerque, New Mexico, USA
    Methods Enzymol 461:227-47. 2009
  10. ncbi request reprint Diazo coupling method for covalent attachment of proteins to solid substrates
    Yang Wu
    Department of Chemical and Nuclear Engineering, University of New Mexico, Albuquerque, New Mexico 87131, USA
    Bioconjug Chem 17:359-65. 2006

Collaborators

  • Alexandre Chigaev
  • Bruce Edwards
  • William E McIntire
  • Zurab Surviladze
  • Tione Buranda
  • Larry A Sklar
  • Gabriel P Lopez
  • Dominique Perez
  • Sireesha Chemburu
  • Menake E Piyasena
  • Stephen D Jett
  • Brian Hjelle
  • Pamela Hall
  • Virginie BonduHawkins
  • Chunyan Ye
  • Richard S Larson
  • Kirk S Schanze
  • David G Whitten
  • Yosune Casana
  • Eunkyung Ji
  • Anna Waller
  • Eric R Prossnitz
  • Sean Biggs
  • Peter C Simons
  • Jinman Huang

Detail Information

Publications18

  1. pmc Discovery of regulators of receptor internalization with high-throughput flow cytometry
    Yang Wu
    Department of Pathology, School of Medicine, University of New Mexico, MSC08 4640, 700 Camino de Salud NE, IDTC Rm 2340, Albuquerque, NM 87131, USA
    Mol Pharmacol 82:645-57. 2012
    ..The results revealed that ligands may be biased with respect to the rate or duration of receptor internalization and that receptor internalization may be independent of activation of the mitogen-activated protein kinase pathway...
  2. pmc High-throughput flow cytometry compatible biosensor based on fluorogen activating protein technology
    Yang Wu
    UNM Center for Molecular Discovery, University of New Mexico School of Medicine, Albuquerque, New Mexico 87131, USA
    Cytometry A 83:220-6. 2013
    ..The system has successfully served as a counter screen assay to eliminate false positive compounds identified in a screen against NIH Molecular Libraries Small Molecule Repository targeting regulators of the human β2AR...
  3. pmc Rapid-mix flow cytometry measurements of subsecond regulation of G protein-coupled receptor ternary complex dynamics by guanine nucleotides
    Yang Wu
    Department of Pathology and Cancer Research Facility, University of New Mexico Health Sciences Center, University of New Mexico, Albuquerque, NM 87131, USA
    Anal Biochem 371:10-20. 2007
    ..These results are compatible with a cell activation model involving G protein conformational changes rather than disassembly of Galphabetagamma heterotrimer...
  4. pmc The development of quantum dot calibration beads and quantitative multicolor bioassays in flow cytometry and microscopy
    Yang Wu
    Department of Pathology and Cancer Center, University of New Mexico, Albuquerque, NM 87131, USA
    Anal Biochem 364:180-92. 2007
    ..The utility of the calibration beads is also extended to the characterization surface densities of dot-labeled epidermal growth factor ligands as well as quantitative indicators of the binding of dot-labeled virus particles to cells...
  5. pmc Spectroscopic characterization of streptavidin functionalized quantum dots
    Yang Wu
    Department of Pathology and Cancer Center, University of New Mexico School of Medicine, Albuquerque, NM 87131, USA
    Anal Biochem 364:193-203. 2007
    ..The results are discussed in the context of bridging the gap between what is currently known in the physical chemistry literature of quantum dots and the quantitative needs of assay development in biological applications...
  6. ncbi request reprint Small-volume rapid-mix device for subsecond kinetic analysis in flow cytometry
    Yang Wu
    Department of Chemical and Nuclear Engineering, University of New Mexico Health Sciences Center, Albuquerque, New Mexico 87131, USA
    Cytometry A 67:37-44. 2005
    ..Although progress has come from improving sample delivery capabilities, little attention has been paid to the volumetric requirements associated with precious biological reagents...
  7. doi request reprint Quantum dots for quantitative flow cytometry
    Tione Buranda
    Department of Pathology and Cancer Center, University of New Mexico School of Medicine, Albuquerque, NM, USA
    Methods Mol Biol 699:67-84. 2011
    ....
  8. pmc Identification of a small GTPase inhibitor using a high-throughput flow cytometry bead-based multiplex assay
    Zurab Surviladze
    University of New Mexico Center for Molecular Discovery, Albuquerque, NM 87131, USA
    J Biomol Screen 15:10-20. 2010
    ..Thus, high-throughput screening via flow cytometry provides a strategy for identifying novel compounds that are active against small GTPases...
  9. doi request reprint Chapter 11. Subsecond analyses of G-protein coupled-receptor ternary complex dynamics by rapid mix flow cytometry
    Tione Buranda
    Department of Pathology and Cancer Center, University of New Mexico Health Science Center, Albuquerque, New Mexico, USA
    Methods Enzymol 461:227-47. 2009
    ..These results are compatible with a cell activation model involving G-protein conformational changes rather than disassembly of Galphabetagamma heterotrimer...
  10. ncbi request reprint Diazo coupling method for covalent attachment of proteins to solid substrates
    Yang Wu
    Department of Chemical and Nuclear Engineering, University of New Mexico, Albuquerque, New Mexico 87131, USA
    Bioconjug Chem 17:359-65. 2006
    ..Results suggest that his-tagged proteins are immobilized by reaction of the his-tag with the diazotized surface, thus offering the possibility for preferential orientation of covalently bound proteins...
  11. pmc Recognition of decay accelerating factor and alpha(v)beta(3) by inactivated hantaviruses: Toward the development of high-throughput screening flow cytometry assays
    Tione Buranda
    Department of Pathology, University of New Mexico, Albuquerque, 87131, USA
    Anal Biochem 402:151-60. 2010
    ..This is a first step toward developing HTS format assays for small molecule inhibitors of viral-cell interactions as well as dissecting the mechanism of infection in a BSL-2 environment...
  12. pmc Real-time partitioning of octadecyl rhodamine B into bead-supported lipid bilayer membranes revealing quantitative differences in saturable binding sites in DOPC and 1:1:1 DOPC/SM/cholesterol membranes
    Tione Buranda
    Department of Pathology and Cancer Center, University of New Mexico School of Medicine, Albuquerque, New Mexico 87131, USA
    J Phys Chem B 114:1336-49. 2010
    ..This approach represents a first step toward a nanoscale probing of membrane heterogeneity in living cells by analyzing differential local FRET among sites of unique receptor expression in living cells...
  13. doi request reprint Real-Time Detection of Protein Trafficking with High-Throughput Flow Cytometry (HTFC) and Fluorogen-Activating Protein (FAP) Base Biosensor
    Yang Wu
    Center for Molecular Discovery, University of New Mexico School of Medicine, Albuquerque, New Mexico Department of Pathology, University of New Mexico School of Medicine, Albuquerque, New Mexico
    Curr Protoc Cytom 67:9.43.1-9.43.11. 2014
    ..In addition, screens of multiplexed targets promise improved efficiency with minor protocol modification. Curr. Protoc. Cytom. 67:9.43.1-9.43.11. © 2014 by John Wiley & Sons, Inc. ..
  14. doi request reprint Conjugated polyelectrolyte supported bead based assays for phospholipase A2 activity
    Sireesha Chemburu
    Department of Chemical and Nuclear Engineering, University of New Mexico, Albuquerque, New Mexico 87131, USA
    J Phys Chem B 112:14492-9. 2008
    ..The sensing of PLA2 activity has been studied both by monitoring fluorescence changes in a multiwell plate reader and by flow cytometry. The assay exhibits good sensitivity with EC50 values in the nanomolar range...
  15. ncbi request reprint Dynamics of fluorescence dequenching of ostrich-quenched fluorescein biotin: a multifunctional quantitative assay for biotin
    Yang Wu
    Department of Chemical and Nuclear Engineering, University of New Mexico, Albuquerque, NM 87131, USA
    Anal Biochem 342:221-8. 2005
    ..The dynamic aspects allow the assay to be extended to a broader range of applications including its use as an indicator of reagent mixing in laminar-flow assays carried out in microfluidic devices...
  16. pmc Discovery of very late antigen-4 (VLA-4, alpha4beta1 integrin) allosteric antagonists
    Alexandre Chigaev
    Department of Pathology and Cancer Center, University of New Mexico Health Sciences Center, Albuquerque, New Mexico 87131, USA
    J Biol Chem 286:5455-63. 2011
    ..Because all the identified compounds are structurally related, previously used, or currently marketed drugs, this result opens a range of therapeutic possibilities for VLA-4-related pathologies...
  17. ncbi request reprint Some mechanistic insights into GPCR activation from detergent-solubilized ternary complexes on beads
    Tione Buranda
    Department of Pathology and Cancer Center, University of New Mexico Health Science Center, Albuquerque, New Mexico 87131, USA
    Adv Protein Chem 74:95-135. 2007
    ..The data and concepts are discussed in the context of a review of current literature on signaling mechanism based on structural and spectroscopic (FRET) studies of ternary complex components...
  18. ncbi request reprint Near-simultaneous and real-time detection of multiple analytes in affinity microcolumns
    Menake E Piyasena
    Cancer Center and Department of Pathology, University of New Mexico School of Medicine, Albuquerque, New Mexico 87131, USA
    Anal Chem 76:6266-73. 2004
    ..g., biomolecular indicators of diseases). Proof-of-principle analytes include FLAG peptide and carcinoembryonic antigen detected at physiologically relevant concentration levels...