Chuanyue Wu

Summary

Affiliation: University of Pittsburgh
Country: USA

Publications

  1. pmc The parvins
    J L Sepulveda
    Department of Pathology, 707B Scaife Hall, University of Pittsburgh, 3550 Terrace Street, Pittsburgh, PA 15261, USA
    Cell Mol Life Sci 63:25-35. 2006
  2. pmc Mechanical signals control SOX-9, VEGF, and c-Myc expression and cell proliferation during inflammation via integrin-linked kinase, B-Raf, and ERK1/2-dependent signaling in articular chondrocytes
    Priyangi M Perera
    Biomechanics and Tissue Engineering Laboratory, The Ohio State University, Postle Hall, 305 W 12th Avenue, Columbus, OH 43210, USA
    Arthritis Res Ther 12:R106. 2010
  3. pmc Focal adhesion: a focal point in current cell biology and molecular medicine
    Chuanyue Wu
    Department of Pathology, University of Pittsburgh, 3550 Terrace Street, Pittsburgh, Pennsylvania 15261, USA
    Cell Adh Migr 1:13-8. 2007
  4. ncbi request reprint Migfilin and its binding partners: from cell biology to human diseases
    Chuanyue Wu
    Department of Pathology, University of Pittsburgh, 707B Scaife Hall, 3550 Terrace Street, Pittsburgh, PA 15261, USA
    J Cell Sci 118:659-64. 2005
  5. ncbi request reprint The PINCH-ILK-parvin complexes: assembly, functions and regulation
    Chuanyue Wu
    Department of Pathology, University of Pittsburgh, 707B Scaife Hall, 3550 Terrace Street, PA 15261, USA
    Biochim Biophys Acta 1692:55-62. 2004
  6. ncbi request reprint PINCH, N(i)ck and the ILK: network wiring at cell-matrix adhesions
    Chuanyue Wu
    Department of Pathology, University of Pittsburgh, 707B Scaife Hall, 3550 Terrace Street, Pittsburgh, Pennsylvania 15261, USA
    Trends Cell Biol 15:460-6. 2005
  7. ncbi request reprint Loss of integrin linked kinase from mouse hepatocytes in vitro and in vivo results in apoptosis and hepatitis
    Vasiliki Gkretsi
    Division of Cellular and Molecular Pathology, Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA
    Hepatology 45:1025-34. 2007
  8. pmc Liver-specific ablation of integrin-linked kinase in mice results in abnormal histology, enhanced cell proliferation, and hepatomegaly
    Vasiliki Gkretsi
    Division of Cellular and Molecular Pathology, Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA
    Hepatology 48:1932-41. 2008
  9. ncbi request reprint Distinct roles of two structurally closely related focal adhesion proteins, alpha-parvins and beta-parvins, in regulation of cell morphology and survival
    Yongjun Zhang
    Department of Pathology, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA
    J Biol Chem 279:41695-705. 2004
  10. ncbi request reprint PINCH-1 is an obligate partner of integrin-linked kinase (ILK) functioning in cell shape modulation, motility, and survival
    Tomohiko Fukuda
    Department of Pathology, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA
    J Biol Chem 278:51324-33. 2003

Research Grants

Collaborators

Detail Information

Publications66

  1. pmc The parvins
    J L Sepulveda
    Department of Pathology, 707B Scaife Hall, University of Pittsburgh, 3550 Terrace Street, Pittsburgh, PA 15261, USA
    Cell Mol Life Sci 63:25-35. 2006
    ..In contrast to PARVA, PARVB inhibits ILK activity and reverses some of its oncogenic effects in cancer cells. This review focuses on the structure and function of the parvins and some possible roles in human diseases...
  2. pmc Mechanical signals control SOX-9, VEGF, and c-Myc expression and cell proliferation during inflammation via integrin-linked kinase, B-Raf, and ERK1/2-dependent signaling in articular chondrocytes
    Priyangi M Perera
    Biomechanics and Tissue Engineering Laboratory, The Ohio State University, Postle Hall, 305 W 12th Avenue, Columbus, OH 43210, USA
    Arthritis Res Ther 12:R106. 2010
    ..In this study, we investigated intracellular mechanisms underlying reparative actions of mechanical signals during inflammation...
  3. pmc Focal adhesion: a focal point in current cell biology and molecular medicine
    Chuanyue Wu
    Department of Pathology, University of Pittsburgh, 3550 Terrace Street, Pittsburgh, Pennsylvania 15261, USA
    Cell Adh Migr 1:13-8. 2007
    ....
  4. ncbi request reprint Migfilin and its binding partners: from cell biology to human diseases
    Chuanyue Wu
    Department of Pathology, University of Pittsburgh, 707B Scaife Hall, 3550 Terrace Street, Pittsburgh, PA 15261, USA
    J Cell Sci 118:659-64. 2005
    ..It probably functions as a key regulator both at cell adhesion sites and nuclei, coordinating multiple cellular processes, and is implicated in the pathogenesis of several human diseases...
  5. ncbi request reprint The PINCH-ILK-parvin complexes: assembly, functions and regulation
    Chuanyue Wu
    Department of Pathology, University of Pittsburgh, 707B Scaife Hall, 3550 Terrace Street, PA 15261, USA
    Biochim Biophys Acta 1692:55-62. 2004
    ....
  6. ncbi request reprint PINCH, N(i)ck and the ILK: network wiring at cell-matrix adhesions
    Chuanyue Wu
    Department of Pathology, University of Pittsburgh, 707B Scaife Hall, 3550 Terrace Street, Pittsburgh, Pennsylvania 15261, USA
    Trends Cell Biol 15:460-6. 2005
    ..Dissecting the interactions and functions that are mediated by the PINCH-1-ILK complex presents an exciting challenge and an opportunity to unravel the structure and functions of the integrin-actin network...
  7. ncbi request reprint Loss of integrin linked kinase from mouse hepatocytes in vitro and in vivo results in apoptosis and hepatitis
    Vasiliki Gkretsi
    Division of Cellular and Molecular Pathology, Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA
    Hepatology 45:1025-34. 2007
    ..CONCLUSION: Our results demonstrate the importance of ILK and integrin signaling for the survival of hepatocytes and the maintenance of normal liver function...
  8. pmc Liver-specific ablation of integrin-linked kinase in mice results in abnormal histology, enhanced cell proliferation, and hepatomegaly
    Vasiliki Gkretsi
    Division of Cellular and Molecular Pathology, Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA
    Hepatology 48:1932-41. 2008
    ..CONCLUSION: Our results show for the first time in vivo the significance of ILK and hepatic ECM-signaling for regulation of hepatocyte proliferation and differentiation...
  9. ncbi request reprint Distinct roles of two structurally closely related focal adhesion proteins, alpha-parvins and beta-parvins, in regulation of cell morphology and survival
    Yongjun Zhang
    Department of Pathology, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA
    J Biol Chem 279:41695-705. 2004
    ..Furthermore, they demonstrate that alpha- and beta-parvins play distinct roles in mammalian cells and suggest that the formation of the ILK-alpha-parvin complex is crucial for protection of cells from apoptosis...
  10. ncbi request reprint PINCH-1 is an obligate partner of integrin-linked kinase (ILK) functioning in cell shape modulation, motility, and survival
    Tomohiko Fukuda
    Department of Pathology, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA
    J Biol Chem 278:51324-33. 2003
    ..These results provide new insights into the functions of PINCH proteins in regulation of ILK and alpha-parvin and control of cell behavior...
  11. ncbi request reprint Molecular dissection of PINCH-1 reveals a mechanism of coupling and uncoupling of cell shape modulation and survival
    Zhen Xu
    Department of Pathology, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA
    J Biol Chem 280:27631-7. 2005
    ..These findings suggest that PINCH-1 functions as a molecular platform for coupling and uncoupling diverse cellular processes via overlapping but yet distinct domain interactions...
  12. ncbi request reprint Formation and phosphorylation of the PINCH-1-integrin linked kinase-alpha-parvin complex are important for regulation of renal glomerular podocyte adhesion, architecture, and survival
    Yanqiang Yang
    707B Scaife Hall, Department of Pathology, University of Pittsburgh, 3550 Terrace Street, Pittsburgh, PA 15261, USA
    J Am Soc Nephrol 16:1966-76. 2005
    ..These results provide important evidence for a crucial role of the PINCH-1-ILK-alpha-parvin complex in the control of podocyte adhesion, morphology, and survival...
  13. pmc Migfilin interacts with Src and contributes to cell-matrix adhesion-mediated survival signaling
    Jianping Zhao
    Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA
    J Biol Chem 284:34308-20. 2009
    ..This migfilin-mediated signaling pathway is dysfunctional in multiple types of carcinoma cells, which likely contributes to aberrant Src activation and anoikis resistance in the cancerous cells...
  14. pmc Reversion-induced LIM interaction with Src reveals a novel Src inactivation cycle
    Yongjun Zhang
    Department of Pathology, University of Pittsburgh, Pittsburgh, PA 15261, USA
    J Cell Biol 184:785-92. 2009
    ..Epigenetic silencing of RIL breaks this Src inactivation cycle and thereby contributes to aberrant Src activation in human cancers...
  15. pmc Tissue-type plasminogen activator promotes murine myofibroblast activation through LDL receptor-related protein 1-mediated integrin signaling
    Kebin Hu
    Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA
    J Clin Invest 117:3821-32. 2007
    ..This study implicates tPA as a fibrogenic cytokine that promotes the progression of kidney fibrosis...
  16. ncbi request reprint Migfilin interacts with vasodilator-stimulated phosphoprotein (VASP) and regulates VASP localization to cell-matrix adhesions and migration
    Yongjun Zhang
    Department of Pathology, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA
    J Biol Chem 281:12397-407. 2006
    ..Our results identify migfilin as an important regulator of cell migration and provide new information on the mechanism by which migfilin regulates this process...
  17. ncbi request reprint PINCH-1 promotes tubular epithelial-to-mesenchymal transition by interacting with integrin-linked kinase
    Yingjian Li
    Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA
    J Am Soc Nephrol 18:2534-43. 2007
    ..We conclude that PINCH-1, through its interaction with ILK, plays an important role in regulating TGF-beta1-mediated EMT and could be a potential future therapeutic target to prevent progression of renal disease...
  18. pmc Kindlin-2 regulates podocyte adhesion and fibronectin matrix deposition through interactions with phosphoinositides and integrins
    Hong Qu
    Department of Pathology, University of Pittsburgh, Pittsburgh, PA 15261, USA
    J Cell Sci 124:879-91. 2011
    ..Our results reveal important functions of kindlin-2 in the regulation of podocyte-matrix adhesion and matrix deposition and shed new light on the mechanism whereby kindlin-2 functions in these processes...
  19. ncbi request reprint PINCH-1 regulates the ERK-Bim pathway and contributes to apoptosis resistance in cancer cells
    Ka Chen
    Departments of Pathology and Pharmacology, University of Pittsburgh School of Medicine, 3550 Terrace Street, Pittsburgh, PA 15261, USA
    J Biol Chem 283:2508-17. 2008
    ..Our results demonstrate a strong dependence of multiple types of apoptosis-resistant cancer cells on PINCH-1 and provide new insights into the molecular mechanism by which cancer cells are protected from apoptosis...
  20. ncbi request reprint Characterization of PINCH-2, a new focal adhesion protein that regulates the PINCH-1-ILK interaction, cell spreading, and migration
    Yongjun Zhang
    Department of Pathology, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA
    J Biol Chem 277:38328-38. 2002
    ..These results identify a novel nuclear and focal adhesion protein that associates with ILK and reveals an important role of PINCH-2 in the regulation of the PINCH-1-ILK interaction, cell shape change, and migration...
  21. pmc Interaction of integrin-linked kinase and miniature chromosome maintenance 7-mediating integrin {alpha}7 induced cell growth suppression
    Yu Chen Han
    Departments of Pathology and Urology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA
    Cancer Res 70:4375-84. 2010
    ..We conclude that ILK interaction with MCM7 and MCM7 phosphorylation may be a critical event in ITGA7 signaling pathway, leading to tumor suppression...
  22. ncbi request reprint The MIG-2/integrin interaction strengthens cell-matrix adhesion and modulates cell motility
    Xiaohua Shi
    Department of Pathology, University of Pittsburgh School of Medicine, 3550 Terrace Street, Pittsburgh, PA 15261, USA
    J Biol Chem 282:20455-66. 2007
    ....
  23. ncbi request reprint TGF-beta1 regulates the PINCH-1-integrin-linked kinase-alpha-parvin complex in glomerular cells
    Kyu Yong Jung
    Department of Pathology, University of Pittsburgh, 3550 Terrace Street, Pittsburgh, PA 15261, USA
    J Am Soc Nephrol 18:66-73. 2007
    ....
  24. pmc Role for integrin-linked kinase in mediating tubular epithelial to mesenchymal transition and renal interstitial fibrogenesis
    Yingjian Li
    Department of Pathology, University of Pittsburgh School of Medicine, S 405 Biomedical Science Tower, 200 Lothrop Street, Pittsburgh, Pennsylvania 15261, USA
    J Clin Invest 112:503-16. 2003
    ..These findings suggest that ILK is a critical mediator for tubular EMT and likely plays a crucial role in the pathogenesis of chronic renal fibrosis...
  25. pmc Phosphorylation of alpha-actinin 4 upon epidermal growth factor exposure regulates its interaction with actin
    Hanshuang Shao
    Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA
    J Biol Chem 285:2591-600. 2010
    ..These results suggest that EGF regulates the actin binding activity of ACTN4 by inducing tyrosyl-directed phosphorylation...
  26. pmc Integrin-linked kinase is involved in matrix-induced hepatocyte differentiation
    Vasiliki Gkretsi
    Department of Cellular and Molecular Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA
    Biochem Biophys Res Commun 353:638-43. 2007
    ..Our results show that ILK and cell-matrix adhesion proteins play an important role in the process of matrix-induced hepatocyte differentiation...
  27. ncbi request reprint Physical and functional association of migfilin with cell-cell adhesions
    Vasiliki Gkretsi
    Department of Pathology, Center for Biological Imaging, University of Pittsburgh, Pittsburgh, PA 15261, USA
    J Cell Sci 118:697-710. 2005
    ..These results identify migfilin as a component of adherens junctions and suggest an important role for migfilin in the organization of the cell-cell adhesion structure...
  28. ncbi request reprint Migfilin and Mig-2 link focal adhesions to filamin and the actin cytoskeleton and function in cell shape modulation
    Yizeng Tu
    Department of Pathology, University of Pittsburgh, Pittsburgh, PA 15261, USA
    Cell 113:37-47. 2003
    ..Together, Mig-2, migfilin and filamin define a connection between cell matrix adhesions and the actin cytoskeleton and participate in the orchestration of actin assembly and cell shape modulation...
  29. pmc CH-ILKBP regulates cell survival by facilitating the membrane translocation of protein kinase B/Akt
    Tomohiko Fukuda
    Department of Pathology, University of Pittsburgh, Pittsburgh, PA 15261, USA
    J Cell Biol 160:1001-8. 2003
    ....
  30. ncbi request reprint Assembly of the PINCH-ILK-CH-ILKBP complex precedes and is essential for localization of each component to cell-matrix adhesion sites
    Yongjun Zhang
    Department of Pathology, University of Pittsburgh, Pittsburgh, PA 15261, USA
    J Cell Sci 115:4777-86. 2002
    ..These results provide new insights into the molecular mechanism underlying the assembly and regulation of cell-matrix adhesion structures...
  31. doi request reprint A suppressive role of mitogen inducible gene-2 in mesenchymal cancer cell invasion
    Xiaohua Shi
    707B Scaife Hall, Department of Pathology, University of Pittsburgh, 3550 Terrace Street, Pittsburgh, PA 15261, USA
    Mol Cancer Res 6:715-24. 2008
    ..Our results reveal an important role of Mig-2 in suppression of mesenchymal cancer cell invasion and shed new light on how altered Mig-2 expression could influence cancer cell invasion...
  32. pmc Roles of PINCH-2 in regulation of glomerular cell shape change and fibronectin matrix deposition
    Xiaohua Shi
    Department of Pathology, University of Pittsburgh, Pittsburgh, PA 15261, USA
    Am J Physiol Renal Physiol 295:F253-63. 2008
    ..g., podocyte survival signaling and mesangial fibronectin matrix deposition), albeit that an aberrantly high level of PINCH-2 may contribute to TGF-beta1-induced alteration in podocyte shape modulation...
  33. pmc Enhanced liver regeneration following changes induced by hepatocyte-specific genetic ablation of integrin-linked kinase
    Udayan Apte
    Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA
    Hepatology 50:844-51. 2009
    ..This is the first evidence of a defect leading to impaired termination of regeneration and excessive accumulation of liver weight following partial hepatectomy...
  34. ncbi request reprint The muscle integrin binding protein (MIBP) interacts with alpha7beta1 integrin and regulates cell adhesion and laminin matrix deposition
    Ji Li
    Department of Pathology, University of Pittsburgh, Pittsburgh, PA 15261, USA
    Dev Biol 261:209-19. 2003
    ..These results suggest that MIBP functions in the control of myogenic differentiation by regulating alpha7beta1 integrin-mediated cell interactions with laminin matrix and intracellular signaling through paxillin...
  35. ncbi request reprint Regulation of fibronectin matrix deposition and cell proliferation by the PINCH-ILK-CH-ILKBP complex
    Lida Guo
    Department of Pathology, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA
    FASEB J 16:1298-300. 2002
    ....
  36. pmc Critical role of filamin-binding LIM protein 1 (FBLP-1)/migfilin in regulation of bone remodeling
    Guozhi Xiao
    Department of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania 15240, USA
    J Biol Chem 287:21450-60. 2012
    ..e., BMSC-extracellular matrix adhesion and migration, cell growth, survival, and differentiation) and the communication between OB/BMSCs and BMMs (i.e., RANKL expression) that controls osteoclastogenesis...
  37. pmc Excessive hepatomegaly of mice with hepatocyte-targeted elimination of integrin linked kinase following treatment with 1,4-bis [2-(3,5-dichaloropyridyloxy)] benzene
    Shashikiran Donthamsetty
    Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA
    Hepatology 53:587-95. 2011
    ..The prolonged proliferative response in the ILK/liver-/- mice seems to be due to sustained induction of CAR leading to sustained induction of c-Myc, which is known to be a key mediator of TCPOPOP-CAR induced direct liver hyperplasia...
  38. pmc Alpha-actinin-4 and CLP36 protein deficiencies contribute to podocyte defects in multiple human glomerulopathies
    Zhongmin Liu
    Department of Pathology, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA
    J Biol Chem 286:30795-805. 2011
    ....
  39. ncbi request reprint Role of the integrin-linked kinase/PINCH1/alpha-parvin complex in cardiac myocyte hypertrophy
    Hua Chen
    Department of Pathology, New York University, New York, NY, USA
    Lab Invest 85:1342-56. 2005
    ..These results suggest that the IPAP1 complex plays an important role in mediating integrin-signaling pathways that regulate cardiac myocyte hypertrophy and resistance to apoptosis...
  40. pmc Expression of integrin-linked kinase and its binding partners in chondrosarcoma: association with prognostic significance
    Dionysios J Papachristou
    Department of Pathology, University of Pittsburgh Medical Center, Pittsburgh, PA, USA
    Eur J Cancer 44:2518-25. 2008
    ..Moreover, our findings highlight the possibilities that ILK might serve as biological marker that could accurately predict a high-grade tumour and that Mig-2 may function as a promising prognostic indicator of high-risk patients...
  41. ncbi request reprint Essential role of integrin-linked kinase in podocyte biology: Bridging the integrin and slit diaphragm signaling
    Chunsun Dai
    Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA
    J Am Soc Nephrol 17:2164-75. 2006
    ..These results also illustrate that the integrin and slit diaphragm signals in podocytes are intrinsically coupled through an ILK-dependent mechanism...
  42. pmc Inhibition of integrin-linked kinase blocks podocyte epithelial-mesenchymal transition and ameliorates proteinuria
    Young Sun Kang
    Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
    Kidney Int 78:363-73. 2010
    ..Our results show that upregulation of ILK is a convergent pathway leading to podocyte EMT, migration, and dysfunction. ILK may be an attractive target for therapeutic intervention of proteinuric kidney diseases...
  43. pmc PINCH1 is transcriptional regulator in podocytes that interacts with WT1 and represses podocalyxin expression
    Dan Wang
    Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, United States of America
    PLoS ONE 6:e17048. 2011
    ..PINCH1 is induced in the fibrotic kidney after injury, and it primarily localizes at the sites of focal adhesion. Whether it can translocate to the nucleus and directly participate in gene regulation is completely unknown...
  44. pmc Fibulin-5 suppresses lung cancer invasion by inhibiting matrix metalloproteinase-7 expression
    Wen Yue
    Department of Pharmacology and Chemical Biolog, University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA
    Cancer Res 69:6339-46. 2009
    ..Collectively, these results suggest that epigenetic silencing of fibulin-5 promotes lung cancer invasion and metastasis by activating MMP-7 expression through the ERK pathway...
  45. ncbi request reprint Analysis of integrin alpha7 mutations in prostate cancer, liver cancer, glioblastoma multiforme, and leiomyosarcoma
    Baoguo Ren
    Department of Pathology, University of Pittsburgh, Pittsburgh, PA, USA
    J Natl Cancer Inst 99:868-80. 2007
    ..Integrins are the major adhesive molecules in mammalian cells. Each integrin subtype plays a unique role in cell differentiation and embryo development. However, integrin involvement in carcinogenesis has not been well defined...
  46. ncbi request reprint NO-dependent osteoclast motility: reliance on cGMP-dependent protein kinase I and VASP
    Beatrice B Yaroslavskiy
    Department of Pathology, University of Pittsburgh and Veteran s Affairs Medical Center, Pittsburgh, PA 15243, USA
    J Cell Sci 118:5479-87. 2005
    ..VASP knockdown also altered distribution of alphavbeta3 at the attachment site. We conclude that PKG I and VASP are essential for reorganization of attachment and cytoplasmic proteins in motility induced by NO or by cGMP...
  47. pmc Protection against Fas-induced fulminant hepatic failure in liver specific integrin linked kinase knockout mice
    Shashikiran Donthamsetty
    Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA
    Comp Hepatol 10:11. 2011
    ..abstract:..
  48. ncbi request reprint Nck-2 interacts with focal adhesion kinase and modulates cell motility
    Silvia M Goicoechea
    Department of Pathology, University of Pittsburgh, 707B Scaife Hall, 3550 Terrace Street, Pittsburgh, PA 15261, USA
    Int J Biochem Cell Biol 34:791-805. 2002
    ..These results identify a novel interaction between Nck-2 and FAK and suggest a role of Nck-2 in the modulation of cell motility...
  49. ncbi request reprint Assembly and signaling of adhesion complexes
    Jorge L Sepulveda
    Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA
    Curr Top Dev Biol 68:183-225. 2005
    ..Finally, we will discuss in detail the structure and regulation of cell-ECM adhesion complexes in cardiac myocytes, which illustrate the importance and complexity of the cell-ECM adhesion structures in organogenesis and diseases...
  50. ncbi request reprint EBV-expressing AGS gastric carcinoma cell sublines present increased motility and invasiveness
    Jareer Kassis
    Department of Pathology, Pittsburgh VAMC and University of Pittsburgh, Pittsburgh, PA, USA
    Int J Cancer 99:644-51. 2002
    ..Instead, we found that the EBV-infected cells presented increased focal adhesion kinase (FAK) phosphorylation. These findings suggest a role for integrin-mediated signaling in promoting EBV-associated invasiveness...
  51. ncbi request reprint Cell-adhesion assays
    Dennis F Kucik
    Research Service, Birmingham VA Medical Center, and Department of Pathology, University of Alabama at Birmingham, Center for Biophysical Sciences and Engineering, Pittsburgh, PA, USA
    Methods Mol Biol 294:43-54. 2005
    ..In addition, variations to the basic protocols and areas where special attention is required for successful application of these methods are discussed...
  52. ncbi request reprint A critical role of the PINCH-integrin-linked kinase interaction in the regulation of cell shape change and migration
    Yongjun Zhang
    Department of Pathology, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA
    J Biol Chem 277:318-26. 2002
    ..These results suggest that PINCH, through its interaction with ILK, is crucially involved in the regulation of cell shape change and motility...
  53. doi request reprint Spatiotemporal response of living cell structures in Dictyostelium discoideum with semiconductor quantum dots
    Lam Helmick
    Department of Mechanical, Carnegie Mellon University, 5000 Forbes Avenue, Pittsburgh, Pennsylvania 15213, USA
    Nano Lett 8:1303-8. 2008
    ....
  54. pmc Integration of cell attachment, cytoskeletal localization, and signaling by integrin-linked kinase (ILK), CH-ILKBP, and the tumor suppressor PTEN
    Sarah Attwell
    BC Cancer Agency, Jack Bell Research Centre, Vancouver, BC, V6H 3Z6, Canada
    Mol Biol Cell 14:4813-25. 2003
    ..These data demonstrate that the kinase and adaptor properties of ILK function together, in a Pi3 kinase-dependent manner, to regulate integrin-mediated cell attachment and signal transduction...
  55. ncbi request reprint Integrin-linked kinase regulates N-WASp-mediated actin polymerization and tension development in tracheal smooth muscle
    Wenwu Zhang
    Department of Cellular and Integrative Physiology, Indiana University School of Medicine, 635 Barnhill Drive, Indianapolis, IN 46202, USA
    J Biol Chem 282:34568-80. 2007
    ..We conclude that the recruitment of the ILK.PINCH.alpha-parvin complex to membrane adhesion complexes is required to initiate cytoskeletal processes required for tension development in smooth muscle...
  56. pmc Selective adapter recruitment and differential signaling networks by VEGF vs. shear stress
    Yingxiao Wang
    Department of Bioengineering and The Whitaker Institute of Biomedical Engineering, University of California at San Diego, La Jolla, CA 92093, USA
    Proc Natl Acad Sci U S A 104:8875-9. 2007
    ..g., ERK and JNK...
  57. ncbi request reprint Structural and functional insights into PINCH LIM4 domain-mediated integrin signaling
    Algirdas Velyvis
    Department of Pharmacology, School of Medicine, Case Western Reserve University, 10500 Euclid Avenue, Cleveland, Ohio 44102, USA
    Nat Struct Biol 10:558-64. 2003
    ..These data provide novel structural insight into LIM domain-mediated protein-protein recognition and demonstrate that the PINCH-Nck2 interaction is an important component of the focal adhesion assembly during integrin signaling...
  58. ncbi request reprint TGF-beta1-induced PINCH-1-ILK-alpha-parvin complex formation regulates mesangial cell proliferation and hypertrophy
    Sung Min Kim
    Department of Pharmacology, Wonkwang University School of Medicine, Iksan 570 749, Korea
    Exp Mol Med 39:514-23. 2007
    ..TGF-beta1-induced alteration of the PIP complex formation may be importantly implicated in the development and progression of glomerular failure shown in several kidney diseases...
  59. ncbi request reprint Structure of an ultraweak protein-protein complex and its crucial role in regulation of cell morphology and motility
    Julia Vaynberg
    Structural Biology Program, Lerner Research Institute, The Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, OH 44195, USA
    Mol Cell 17:513-23. 2005
    ..Together, the data provide a molecular basis for an ultraweak PPI in regulating focal adhesion dynamics during integrin signaling...
  60. pmc The structure of alpha-parvin CH2-paxillin LD1 complex reveals a novel modular recognition for focal adhesion assembly
    Xiaoxia Wang
    Structural Biology Program, Lerner Research Institute, Cleveland Clinic, 9500 Euclid Avenue, Cleveland, OH 44195, USA
    J Biol Chem 283:21113-9. 2008
    ..These results define a novel modular recognition mode and reveal how alpha-parvin associates with paxillin to mediate the FA assembly and signaling...
  61. ncbi request reprint Integrin-linked kinase regulates Bergmann glial differentiation during cerebellar development
    Richard Belvindrah
    The Scripps Research Institute, Department of Cell Biology, Institute for Childhood and Neglected Disease, 10550 N Torrey Pines Road, La Jolla, CA 92037, USA
    Mol Cell Neurosci 33:109-25. 2006
    ..We also show that granule cell precursor proliferation is affected in ILK-deficient mice, but our findings provide strong evidence that proliferative defects are a secondary consequence of ILK function in glia...
  62. ncbi request reprint Use of an aggressive MCF-7 cell line variant, TMX2-28, to study cell invasion in breast cancer
    Joseph M Gozgit
    Department of Veterinary and Animal Sciences, University of Massachusetts, Amherst, Massachusetts, USA
    Mol Cancer Res 4:905-13. 2006
    ..Examination of 30 frozen breast tumors supported the finding that MIG2 is overexpressed in a subset of breast cancers. We suggest that MIG2's normal regulation and function are disrupted in breast cancer...
  63. ncbi request reprint Expression and significance of integrin-linked kinase in cultured cells, normal tissue, and diseased tissue of aging rat kidneys
    Zhihui Li
    Department of Nephrology, Kidney Center and Key Lab of PLA, Chinese General Hospital of PLA, Beijing, PR China
    J Gerontol A Biol Sci Med Sci 59:984-96. 2004
    ..The results suggest that ILK may be involved in the fibrotic or senescent process in the aging kidney...
  64. ncbi request reprint Integrin-linked kinase induces both senescence-associated alterations and extracellular fibronectin assembly in aging cardiac fibroblasts
    XiangMei Chen
    Department of Nephrology, Kidney Center and Key Lab of PLA, Chinese General Hospital of PLA, Beijing, PR China
    J Gerontol A Biol Sci Med Sci 61:1232-45. 2006
    ..The results indicate that ILK can accelerate the process of cellular senescence...
  65. pmc Kindlin-2 (Mig-2): a co-activator of beta3 integrins
    Yan Qing Ma
    Department of Molecular Cardiology, Cleveland Clinic, Cleveland, OH 44195, USA
    J Cell Biol 181:439-46. 2008
    ..Our results thus identify kindlin-2 as a novel regulator of integrin activation; it functions as a coactivator...
  66. ncbi request reprint ILK mediates actin filament rearrangements and cell migration and invasion through PI3K/Akt/Rac1 signaling
    Yong Qian
    Pathology and Physiology Research Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, WV 26506, USA
    Oncogene 24:3154-65. 2005
    ..Taken together, our results identify a mechanism through which ILK can regulate both integrin-associated rearrangements of actin filaments and cell migration and invasion at the integrin receptor-proximal region...

Research Grants21

  1. Kindlin-2 in Cell-Matrix Adhesion and Signaling
    Chuanyue Wu; Fiscal Year: 2011
    ..These studies may lead to identification of novel therapeutic targets to control diseases associated with abnormal cell-ECM adhesion and growth. ..
  2. PINCH-1 Interactions and Functions
    Chuanyue Wu; Fiscal Year: 2010
    ..Ultimately, these studies may lead to novel therapeutic approaches to control pathological processes in the liver and other organs that are associated with abnormal cell-ECM adhesion, migration and apoptosis. ..
  3. The PINCH-ILK-parvin complexes in glomerular cells
    Chuanyue Wu; Fiscal Year: 2010
    ..The proposed studies, therefore, not only will advance our understanding of the molecular mechanisms that govern glomerular cell behavior but also may lead to novel therapeutic approaches that alleviate progressive glomerular failure. ..
  4. The PINCH-ILK-parvin complexes in glomerular cells
    Chuanyue Wu; Fiscal Year: 2009
    ..The proposed studies, therefore, not only will advance our understanding of the molecular mechanisms that govern glomerular cell behavior but also may lead to novel therapeutic approaches that alleviate progressive glomerular failure. ..
  5. PINCH-1 Interactions and Functions
    Chuanyue Wu; Fiscal Year: 2007
    ..Ultimately, these studies may lead to novel therapeutic approaches to control pathological processes in the liver and other organs that are associated with abnormal cell-ECM adhesion, migration and apoptosis. ..
  6. ILK Signaling in Renal Growth and Matrix Deposition
    Chuanyue Wu; Fiscal Year: 2005
    ..abstract_text> ..
  7. ILK SIGNALING--RENAL CELL GROWTH AND MATRIX DEPOSITION
    Chuanyue Wu; Fiscal Year: 2001
    ....
  8. Assembly and Functions of the PINCH/ILK/CH-ILKBP Complex
    Chuanyue Wu; Fiscal Year: 2005
    ....