Research Topics
Genomes and Genes | STEPHEN H contact WRIGHTSummaryAffiliation: University of Arizona Country: USA Publications
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Publications
Multiple mechanisms of ligand interaction with the human organic cation transporter, OCT2Jaclyn N Harper
Univ of Arizona College of Medicine, Dept of Physiology, PO Box 245051, Tucson, AZ 85724
Am J Physiol Renal Physiol 304:F56-67. 2013..The results underscore the caution required for development of predictive models of ligand interaction with multidrug transporters...
Molecular determinants of ligand selectivity for the human multidrug and toxin extruder proteins MATE1 and MATE2-KBETHZAIDA ASTORGA
Department of Physiology, University of Arizona, Tucson, AZ 85724, USA
J Pharmacol Exp Ther 341:743-55. 2012....
Role of organic cation transporters in the renal handling of therapeutic agents and xenobioticsStephen H Wright
Department of Physiology, College of Medicine, University of Arizona, Tucson, AZ 85724, USA
Toxicol Appl Pharmacol 204:309-19. 2005..The characterization of their activity, and their localization within distinct regions of the kidney, has permitted development of models describing the molecular and cellular basis of the renal secretion of OCs...- Generation of resting membrane potentialStephen H Wright
Department of Physiology, College of Medicine, University of Arizona, Tucson, AZ 85724, USA
Adv Physiol Educ 28:139-42. 2004..The intention is to provide students a general view of the quantitative relationship that exists between 1) transmembrane gradients for K(+) and Na(+) and 2) the relative channel-mediated permeability of the membrane to these ions... - Molecular and cellular physiology of renal organic cation and anion transportStephen H Wright
Dept of Physiology, College of Medicine, Univ of Arizona, Tucson, AZ 85724, USA
Physiol Rev 84:987-1049. 2004.... - Functional map of TEA transport activity in isolated rabbit renal proximal tubulesStephen H Wright
Department of Physiology, University of Arizona, Tucson, Arizona 85724, USA
Am J Physiol Renal Physiol 287:F442-51. 2004..These data indicate that renal OC transport involves the concerted activity of a suite of transport processes... - Influence of substrate structure on substrate binding to the renal organic cation/H+ exchangerS H Wright
Department of Physiology, College of Medicine, University of Arizona, Tucson, AZ 85724, USA
Pflugers Arch 437:603-10. 1999..Development of the QSAR and receptor surface model open the way to quantitative tests of the specific physical and structural determinants of substrate selectivity by the renal OC/H+ exchanger... - A conserved glutamate residue in transmembrane helix 10 influences substrate specificity of rabbit OCT2 (SLC22A2)Xiaohong Zhang
Department of Physiology, University of Arizona, Tucson, Arizona 85724, USA
J Biol Chem 280:34813-22. 2005..The results suggest that homology modeling offers an opportunity to direct future site-directed studies of OCT/substrate interaction... - MATE1 has an external COOH terminus, consistent with a 13-helix topologyXiaohong Zhang
Department of Physiology, College of Medicine, University of Arizona, Tucson, Arizona 85724, USA
Am J Physiol Renal Physiol 297:F263-71. 2009..These data are consistent with a mammalian MATE topology that includes 13 TMHs and indicate that the terminal TMH, although not necessary for transport function, may influence the turnover characteristics of the transporter... - Molecular identification and functional characterization of rabbit MATE1 and MATE2-KXiaohong Zhang
Dept of Physiology, College of Medicine, University of Arizona, Tucson, AZ 85724, USA
Am J Physiol Renal Physiol 293:F360-70. 2007..Overall, the selectivity of MATE1 and MATE2-K correlated closely with that observed in rabbit renal brush-border membrane vesicles... - Relative contribution of OAT and OCT transporters to organic electrolyte transport in rabbit proximal tubuleXiaohong Zhang
Dept. of Physiology, College of Medicine, University of Arizona, Tucson, AZ 85724, USA
Am J Physiol Renal Physiol 287:F999-1010. 2004..Thus the fractional contribution of different OE transporters to renal secretion is influenced by their affinity for substrate and relative expression level in RPT... - The human organic cation transporter (hOCT2) recognizes the degree of substrate ionizationWendy M Barendt
Department of Physiology, University of Arizona, Tucson, Arizona 85724, USA
J Biol Chem 277:22491-6. 2002..We conclude that the degree of ionization plays a critical role in binding of substrate to organic cation transporters... - Molecular determinants of substrate/inhibitor binding to the human and rabbit renal organic cation transporters hOCT2 and rbOCT2Wendy M Suhre
Department of Physiology, College of Medicine, University of Arizona, Tucson, AZ 85724, USA
Mol Pharmacol 67:1067-77. 2005..The current models enabled prediction of OCT2 affinity and may prove useful in the prediction of unwanted drug interactions at the level of the renal secretory process... - Molecular cloning of rabbit organic cation transporter rbOCT2 and functional comparisons with rbOCT1Xiaohong Zhang
Department of Physiology, College of Medicine, University of Arizona, Tucson, Arizona 85724, USA
Am J Physiol Renal Physiol 283:F124-33. 2002..The median IC50 for CIM inhibition of TEA uptake was 12.3 microM, suggesting that OCT2 is the major contributor to basolateral organic cation transport in the S2 segment of proximal tubule in rabbit kidney... - Influence of estrogen and xenoestrogens on basolateral uptake of tetraethylammonium by opossum kidney cells in cultureRyan M Pelis
Department of Physiology, The University of Arizona, Tucson, AZ 85724, USA
J Pharmacol Exp Ther 323:555-61. 2007..This reduction in transport capacity was correlated with a reduction in OC transporter OCT1 protein expression following treatment with both agents... - Inorganic mercury interacts with cysteine residues (C451 and C474) of hOCT2 to reduce its transport activityRyan M Pelis
Department of Physiology, University of Arizona, College of Medicine, Tucson, Arizona 85724, USA
Am J Physiol Renal Physiol 292:F1583-91. 2007..These data indicate that C451 and C474 of hOCT2 reside in the aqueous milieu of the cleft and that interaction of Hg(2+) with these residues causes reduced TEA transport activity... - Cysteine accessibility in the hydrophilic cleft of human organic cation transporter 2Ryan M Pelis
Department of Physiology, College of Medicine, University of Arizona, Tucson, Arizona 85743, USA
J Biol Chem 281:35272-80. 2006..These data demonstrate that cysteine 474 of OCT2 is exposed to the aqueous milieu of the cleft and contributes to forming a pathway for organic cation transport... - Functional influence of N-glycosylation in OCT2-mediated tetraethylammonium transportRyan M Pelis
Dept of Physiology, College of Medicine, Univ of Arizona, Tucson, AZ 85724, USA
Am J Physiol Renal Physiol 290:F1118-26. 2006.... - Sex differences in the mRNA, protein, and functional expression of organic anion transporter (Oat) 1, Oat3, and organic cation transporter (Oct) 2 in rabbit renal proximal tubulesCarlotta E Groves
Department of Physiology, University of Arizona, Tucson, 85724, USA
J Pharmacol Exp Ther 316:743-52. 2006..Thus, unlike rodents, rabbit renal OA and OC transport does not exhibit sex differences, pointing to the need for caution in extrapolating transport-related sex differences between species... - The molecular and cellular physiology of basolateral organic anion transport in mammalian renal tubulesWilliam H Dantzler
Department of Physiology, Health Science Center, College of Medicine, University of Arizona, Tucson, AZ 85724 5051, USA
Biochim Biophys Acta 1618:185-93. 2003.... - Interaction of cysteine conjugates with human and rabbit organic anion transporter 1Carlotta E Groves
Department of Physiology, College of Medicine, University of Arizona, Tucson, Arizona 85724, USA
J Pharmacol Exp Ther 304:560-6. 2003..Finally, preloading cells transfected with hOAT1 with BTC significantly trans-stimulated the uptake of PAH, consistent with the conclusion that BTC and, hence, other cysteine S-conjugates are substrates for hOAT1... - Localization of multidrug resistance-associated protein 2 in the nonpigmented ciliary epithelium of the eyeRyan M Pelis
Department of Physiology, University of Arizona, College of Medicine, Tucson, Arizona, USA
J Pharmacol Exp Ther 329:479-85. 2009..MRP2 at the apical membrane of NPE cells may be involved in protecting intraocular tissues from exposure to potentially harmful toxins... - Interaction of H+ with the extracellular and intracellular aspects of hMATE1Yodying Dangprapai
Dept of Physiology, Univ of Arizona, Tucson, 85724, USA
Am J Physiol Renal Physiol 301:F520-8. 2011..5 nM (pH 7.91), consistent with symmetrical interactions of H(+) with the inward-facing and outward-facing aspects of hMATE1... - Influence of molecular structure on substrate binding to the human organic cation transporter, hOCT1Dallas Bednarczyk
Department of Physiology, University of Arizona, Tucson, Arizona, USA
Mol Pharmacol 63:489-98. 2003..These results indicate how a combination of computational and in vitro approaches may yield insight into the binding affinity of transporters and may be applicable to predicting these properties for new therapeutics... - Functional mapping of rbOCT1 and rbOCT2 activity in the S2 segment of rabbit proximal tubuleSanti Kaewmokul
Department of Physiology, Mahidol University, Bangkok, 10700 Thailand
Am J Physiol Renal Physiol 285:F1149-59. 2003.... - The renal Na(+)-dependent dicarboxylate transporter, NaDC-3, translocates dimethyl- and disulfhydryl-compounds and contributes to renal heavy metal detoxificationBirgitta C Burckhardt
Zentrum Physiologie und Pathophysiologie, Abteilung Vegetative Physiologie und Pathophysiologie, Georg August Universitat, Gottingen, Germany
J Am Soc Nephrol 13:2628-38. 2002..The data suggest that NaDC-3 is an essential component in the delivery of uncomplexed antidotes for renal heavy metal detoxification... - Pentavalent arsenic can bind to biomoleculesAndrea Raab
University of Aberdeen, College of Physical Sciences, Aberdeen, UK
Angew Chem Int Ed Engl 46:2594-7. 2007
Research Grants
- Moleculer Organization of Renal Organic Cation TransportStephen H Wright; Fiscal Year: 2010..The results of these studies will help predict and, ideally, preempt unwanted drug-drug interactions in both the kidney and liver, and can be expected to assist in development of programs of rational drug design. ..
- Molecular Organization or Renal Organic Anion TransportStephen Wright; Fiscal Year: 2007....
- Moleculer Organization of Renal Organic Cation TransportStephen Wright; Fiscal Year: 2007..Application of the information gained through these studies holds the promise of predicting potential drug-interactions and the basis of genetic differences in renal secretion of cationic xenobiotics. ..
- Molecular Organization or Renal Organic Anion TransportStephen Wright; Fiscal Year: 2009..abstract_text> ..
- Molecular Organization or Renal Organic Anion TransportStephen H Wright; Fiscal Year: 2010..abstract_text> ..
- MOLECULAR ORGANIZATION OF RENAL ORGANIC CATION TRANSPORTStephen Wright; Fiscal Year: 2003..abstract_text> ..
- RENAL TRANSPORT OF ORGANIC CHELATORS OF HEAVY METALSStephen Wright; Fiscal Year: 2004..The proposed studies will result in a new, general model of the cellular strategy for secretion of a diverse array of xenobiotic OAs, and a more specific understanding of the action of heavy metal chelators on renal cells. ..
- Molecular Organization of the Organic cation-Proton Exchanger, MATE1STEPHEN H contact WRIGHT; Fiscal Year: 2010..The results of these studies will help predict and, ideally, preempt unwanted drug-drug interactions in both the kidney and liver, and can be expected to assist in development of programs of structure-based rational drug design. ..