Brent L Wood

Summary

Affiliation: University of Washington
Country: USA

Publications

  1. ncbi request reprint 9-color and 10-color flow cytometry in the clinical laboratory
    Brent Wood
    Department of Laboratory Medicine, University of Washington School of Medicine, Seattle, WA 98195, USA
    Arch Pathol Lab Med 130:680-90. 2006
  2. ncbi request reprint Four-color flow cytometry shows strong concordance with bone marrow morphology and cytogenetics in the evaluation for myelodysplasia
    Steven J Kussick
    Department of Laboratory Medicine, University of Washington, Seattle, USA
    Am J Clin Pathol 124:170-81. 2005
  3. ncbi request reprint Myeloid malignancies: myelodysplastic syndromes, myeloproliferative disorders, and acute myeloid leukemia
    Brent L Wood
    Hematopathology Laboratory, Box 357110, Department of Laboratory Medicine, University of Washington Medical Center, 1959 NE Pacific Street, Seattle, WA 98195, USA
    Clin Lab Med 27:551-75, vii. 2007
  4. ncbi request reprint 2006 Bethesda International Consensus recommendations on the immunophenotypic analysis of hematolymphoid neoplasia by flow cytometry: optimal reagents and reporting for the flow cytometric diagnosis of hematopoietic neoplasia
    Brent L Wood
    Department of Laboratory Medicine, University of Washington Medical Center, Seattle, Washington 98195, USA
    Cytometry B Clin Cytom 72:S14-22. 2007
  5. doi request reprint Ten-color immunophenotyping of hematopoietic cells
    Brent L Wood
    University of Washington, Seattle, Washington, USA
    Curr Protoc Cytom . 2005
  6. ncbi request reprint Interactions between mouse IgG2 antibodies are common and mediated by plasma C1q
    Brent L Wood
    Department of Laboratory Medicine, University of Washington Medical Center, Seattle, Washington 98195, USA
    Cytometry B Clin Cytom 70:321-8. 2006
  7. pmc Hematopoietic stem cell expansion facilitates multilineage engraftment in a nonhuman primate cord blood transplantation model
    Korashon L Watts
    Clinical Research Division, Fred Hutchinson Cancer Research Center FHCRC, Seattle, Wash, USA
    Exp Hematol 40:187-96. 2012
  8. ncbi request reprint Distinct hematopoietic stem/progenitor cell populations are responsible for repopulating NOD/SCID mice compared with nonhuman primates
    Peter A Horn
    Fred Hutchinson Cancer Research Center, D1 100, PO Box 19024, 1100 Fairview Ave N, Seattle, WA 98109 1024, USA
    Blood 102:4329-35. 2003
  9. doi request reprint Flow cytometric evaluation of skin biopsies for mycosis fungoides
    Chris H Jokinen
    Department of Laboratory Medicine, University of Washington Medical Center, Box 357110 1959 NE Pacific St, Seattle, WA, 91895, USA
    Am J Dermatopathol 33:483-91. 2011
  10. pmc Significance of minimal residual disease before myeloablative allogeneic hematopoietic cell transplantation for AML in first and second complete remission
    Roland B Walter
    Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109 1924, USA
    Blood 122:1813-21. 2013

Detail Information

Publications44

  1. ncbi request reprint 9-color and 10-color flow cytometry in the clinical laboratory
    Brent Wood
    Department of Laboratory Medicine, University of Washington School of Medicine, Seattle, WA 98195, USA
    Arch Pathol Lab Med 130:680-90. 2006
    ..The development of commercial flow cytometers capable of detecting more than 10 simultaneous fluorescent signals presents opportunities for improved diagnosis and monitoring of patients with leukemia and lymphoma...
  2. ncbi request reprint Four-color flow cytometry shows strong concordance with bone marrow morphology and cytogenetics in the evaluation for myelodysplasia
    Steven J Kussick
    Department of Laboratory Medicine, University of Washington, Seattle, USA
    Am J Clin Pathol 124:170-81. 2005
    ....
  3. ncbi request reprint Myeloid malignancies: myelodysplastic syndromes, myeloproliferative disorders, and acute myeloid leukemia
    Brent L Wood
    Hematopathology Laboratory, Box 357110, Department of Laboratory Medicine, University of Washington Medical Center, 1959 NE Pacific Street, Seattle, WA 98195, USA
    Clin Lab Med 27:551-75, vii. 2007
    ..Deviation from this pattern is a hallmark of hematopoietic neoplasia. Application of these principles to myelodysplastic syndromes, myeloproliferative disorders, and acute myeloid leukemia is presented and illustrated...
  4. ncbi request reprint 2006 Bethesda International Consensus recommendations on the immunophenotypic analysis of hematolymphoid neoplasia by flow cytometry: optimal reagents and reporting for the flow cytometric diagnosis of hematopoietic neoplasia
    Brent L Wood
    Department of Laboratory Medicine, University of Washington Medical Center, Seattle, Washington 98195, USA
    Cytometry B Clin Cytom 72:S14-22. 2007
    ..The 2006 Bethesda Consensus conference represents the first successful attempt to define a set of consensus reagents suitable for the initial evaluation of hematopoietic neoplasia...
  5. doi request reprint Ten-color immunophenotyping of hematopoietic cells
    Brent L Wood
    University of Washington, Seattle, Washington, USA
    Curr Protoc Cytom . 2005
    ....
  6. ncbi request reprint Interactions between mouse IgG2 antibodies are common and mediated by plasma C1q
    Brent L Wood
    Department of Laboratory Medicine, University of Washington Medical Center, Seattle, Washington 98195, USA
    Cytometry B Clin Cytom 70:321-8. 2006
    ..The simultaneous use of multiple antibodies for multicolor flow cytometry is increasingly common and presents the opportunity for antibody interactions...
  7. pmc Hematopoietic stem cell expansion facilitates multilineage engraftment in a nonhuman primate cord blood transplantation model
    Korashon L Watts
    Clinical Research Division, Fred Hutchinson Cancer Research Center FHCRC, Seattle, Wash, USA
    Exp Hematol 40:187-96. 2012
    ..Furthermore, it can be used in long-term follow-up studies to determine how specific expansion techniques affect engraftment of various hematopoietic lineages...
  8. ncbi request reprint Distinct hematopoietic stem/progenitor cell populations are responsible for repopulating NOD/SCID mice compared with nonhuman primates
    Peter A Horn
    Fred Hutchinson Cancer Research Center, D1 100, PO Box 19024, 1100 Fairview Ave N, Seattle, WA 98109 1024, USA
    Blood 102:4329-35. 2003
    ..These results suggest that distinct hematopoietic stem/progenitor cells are responsible for hematopoietic reconstitution in NOD/SCID mice compared with nonhuman primates...
  9. doi request reprint Flow cytometric evaluation of skin biopsies for mycosis fungoides
    Chris H Jokinen
    Department of Laboratory Medicine, University of Washington Medical Center, Box 357110 1959 NE Pacific St, Seattle, WA, 91895, USA
    Am J Dermatopathol 33:483-91. 2011
    ..The high specificity of FC suggests that it will be a useful adjunct to routine histology in the evaluation of diagnostic skin biopsies for MF...
  10. pmc Significance of minimal residual disease before myeloablative allogeneic hematopoietic cell transplantation for AML in first and second complete remission
    Roland B Walter
    Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109 1924, USA
    Blood 122:1813-21. 2013
    ..90 times higher for MRD(pos) patients (P < .001). Together, our findings indicate that the negative impact of pre-HCT MRD is similar for AML in CR1 and CR2 with even minute levels (≤ 0.1%) as being associated with adverse outcome...
  11. ncbi request reprint Anatomical compartments modify the response of human hematopoietic cells to a mitogenic signal
    Yasuo Nagasawa
    Department of Medicine, K260 Health Sciences Building, Mailstop 357710, University of Washington, Seattle, Washington 98195, USA
    Stem Cells 24:908-17. 2006
    ..These findings establish CIDs as in vivo growth factors for human hematopoietic cells...
  12. ncbi request reprint Identification and purification of classical Hodgkin cells from lymph nodes by flow cytometry and flow cytometric cell sorting
    Jonathan R Fromm
    Department of Laboratory Medicine, University of Washington, WA 98195, USA
    Am J Clin Pathol 126:764-80. 2006
    ..FC may offer an alternative to immunohistochemical analysis in confirming the diagnosis of CHL in certain cases, and, through cell sorting, it provides a means of rapidly isolating pure HRS cells...
  13. pmc High incidence of leukemia in large animals after stem cell gene therapy with a HOXB4-expressing retroviral vector
    Xiao Bing Zhang
    Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109 1024, USA
    J Clin Invest 118:1502-10. 2008
    ..Our data thus suggest the continued need for caution in genetic manipulation of repopulating cells, particularly when the transgene might impart an intrinsic growth advantage...
  14. ncbi request reprint Strategies for immunophenotyping and purifying classical Hodgkin lymphoma cells from lymph nodes by flow cytometry and flow cytometric cell sorting
    Jonathan R Fromm
    Department of Laboratory Medicine, University of Washington, Seattle 98195, USA
    Methods 57:368-75. 2012
    ..This manuscript provides experimental protocols for immunophenotyping CHL by flow cytometry as well as purifying the HRS cells via flow cytometric cell sorting...
  15. ncbi request reprint Four-color flow cytometry identifies virtually all cytogenetically abnormal bone marrow samples in the workup of non-CML myeloproliferative disorders
    Steven J Kussick
    Department of Laboratory Medicine, University of Washington, Seattle, USA
    Am J Clin Pathol 120:854-65. 2003
    ..In experienced laboratories, 4-color flow cytometry represents a useful method to help distinguish benign from neoplastic marrow in the workup of non-CML MPDs...
  16. ncbi request reprint Using 4-color flow cytometry to identify abnormal myeloid populations
    Steven J Kussick
    University of Washington Department of Laboratory Medicine, Seattle, Wash, USA
    Arch Pathol Lab Med 127:1140-7. 2003
    ....
  17. doi request reprint Flow cytometry can diagnose classical hodgkin lymphoma in lymph nodes with high sensitivity and specificity
    Jonathan R Fromm
    Department of Laboratory Medicine, University of Washington, Seattle, WA 98195, USA
    Am J Clin Pathol 131:322-32. 2009
    ..With the current availability of 8 (or more) color clinical flow cytometers, this assay can now be applied to routinely immunophenotype and confirm a diagnosis of CHL or as an adjunct to immunohistochemical analysis...
  18. doi request reprint Flow cytometric evaluation of CD38 expression assists in distinguishing follicular hyperplasia from follicular lymphoma
    Kristin Mantei
    Department of Laboratory Medicine, University of Washington Medical Center, Seattle, Washington 98195, USA
    Cytometry B Clin Cytom 76:315-20. 2009
    ..0E-16) in FL in comparison with FH, as has been previously reported. This study identified decreased CD38 as a common finding in FL in comparison with FH and provides an additional tool to help differentiate FL from FH by flow cytometry...
  19. ncbi request reprint High-throughput sequencing detects minimal residual disease in acute T lymphoblastic leukemia
    David Wu
    Department of Laboratory Medicine, University of Washington, Seattle, WA 98109, USA
    Sci Transl Med 4:134ra63. 2012
    ..Thus, next-generation sequencing of lymphoid receptor gene repertoire may improve clinical diagnosis and subsequent MRD monitoring of lymphoproliferative disorders...
  20. ncbi request reprint A six-color flow cytometry assay for immunophenotyping classical Hodgkin lymphoma in lymph nodes
    Jonathan R Fromm
    University of Washington Medical Center, Box 357110, 1959 NE Pacific St, Seattle, WA 98195 e mail
    Am J Clin Pathol 141:388-96. 2014
    ..Therefore, a six-color FC tube was designed employing the following combination: CD64-FITC/CD30-PE/CD40-PeCy5.5/CD20-PECy7/CD95-APC/CD3-APC-H7...
  21. doi request reprint Flow cytometry for non-Hodgkin and classical Hodgkin lymphoma
    David Wu
    Department of Laboratory Medicine, University of Washington, Seattle, WA, USA
    Methods Mol Biol 971:27-47. 2013
    ..We highlight an overview of procedures for processing and immunophenotyping non-Hodgkin B- and T-cell lymphomas and also describe our strategy for the sensitive and specific diagnosis of classical Hodgkin lymphoma...
  22. ncbi request reprint "Double-Hit" mature B-cell lymphomas show a common immunophenotype by flow cytometry that includes decreased CD20 expression
    David Wu
    University of Washington Medical Center, Seattle Cancer Care Alliance, Seattle, WA 98109, USA
    Am J Clin Pathol 134:258-65. 2010
    ....
  23. ncbi request reprint Lineage-specific identification of nonhematopoietic neoplasms by flow cytometry
    Anthony Chang
    University of Washington, Department of Laboratory Medicine, 1959 NE Pacific St, Seattle, WA 98195, USA
    Am J Clin Pathol 119:643-55. 2003
    ....
  24. pmc Immaturity associated antigens are lost during induction for T cell lymphoblastic leukemia: implications for minimal residual disease detection
    Mikhail Roshal
    Department of Laboratory Medicine, University of Washington Seattle, Washington, USA
    Cytometry B Clin Cytom 78:139-46. 2010
    ..Little information is available about the stability of these antigens in precursor T cell ALL (T-ALL) though it is presumed that CD99 and terminal deoxynucleotidyl transferase (TdT) are highly informative based on limited studies...
  25. ncbi request reprint Prominent clonal B-cell populations identified by flow cytometry in histologically reactive lymphoid proliferations
    Steven J Kussick
    Department of Laboratory Medicine, University of Washington, Seattle, USA
    Am J Clin Pathol 121:464-72. 2004
    ..Our findings add rare cases of follicular hyperplasia to the list of histologically reactive settings in which clonal B-cell populations might be present...
  26. pmc Impact of pretransplantation minimal residual disease, as detected by multiparametric flow cytometry, on outcome of myeloablative hematopoietic cell transplantation for acute myeloid leukemia
    Roland B Walter
    Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109 1024, USA
    J Clin Oncol 29:1190-7. 2011
    ..Hitherto, little attention has been given to the prognostic impact of pretransplantation minimal residual disease (MRD)...
  27. ncbi request reprint High-dose chemo-radioimmunotherapy with autologous stem cell support for relapsed mantle cell lymphoma
    Ajay K Gopal
    Clinical Research Division, Fred Hutchinson Cancer Research Center, University of Washington, Seattle, WA, USA
    Blood 99:3158-62. 2002
    ..High-dose treatment with (131)I-Tositumomab, etoposide, and cyclophosphamide results in a high remission rate and may provide long-term disease-free survival for patients with relapsed or refractory mantle cell lymphoma...
  28. ncbi request reprint Decreased NK cell frequency in chronic hepatitis C does not affect ex vivo cytolytic killing
    Chihiro Morishima
    Department of Pediatrics, University of Washington School of Medicine, Seattle, WA, USA
    Hepatology 43:573-80. 2006
    ..035). In conclusion, NK cell cytolytic function does not appear to be impaired in chronic hepatitis C, but higher levels of NK cell cytolysis are associated with less liver fibrosis...
  29. ncbi request reprint Mobilization of hematopoietic progenitor cells in healthy volunteers by AMD3100, a CXCR4 antagonist
    W Conrad Liles
    Department of Medicine, University of Washington, HSB AA 522, Box 356422, Seattle, WA 98195
    Blood 102:2728-30. 2003
    ..AMD3100 was well tolerated and caused only mild, transient toxicity. These findings suggest potential clinical application of AMD3100 for CD34+ cell mobilization and collection for hematopoietic stem cell transplantation...
  30. ncbi request reprint Augmented mobilization and collection of CD34+ hematopoietic cells from normal human volunteers stimulated with granulocyte-colony-stimulating factor by single-dose administration of AMD3100, a CXCR4 antagonist
    W Conrad Liles
    Department of Medicine, University of Washington, Seattle, Washington 98195, USA
    Transfusion 45:295-300. 2005
    ..AMD3100, a selective antagonist of CXCR4, rapidly mobilizes CD34+ hematopoietic progenitor cells (HPCs) from marrow to peripheral blood with minimal side effects...
  31. doi request reprint Flow cytometric monitoring of residual disease in acute leukemia
    Brent L Wood
    Department of Laboratory Medicine, University of Washington, Seattle, WA, USA
    Methods Mol Biol 999:123-36. 2013
    ..Principles of data analysis that allow for the reproducible detection of small populations of abnormal hematopoietic cells in peripheral blood and bone marrow are presented...
  32. ncbi request reprint Lymphomatoid papulosis with CD1a+ dendritic cell hyperplasia, mimicking Langerhans cell histiocytosis
    Chris H Jokinen
    Department of Pathology, University of Washington, Seattle, WA 98195, USA
    J Cutan Pathol 34:584-7. 2007
    ....
  33. pmc Blood and gastric FOXP3+ T cells are not decreased in human gastric graft-versus-host disease
    James D Lord
    Department of Medicine, University of Washington School of Medicine, Seattle, WA 98101, USA
    Biol Blood Marrow Transplant 17:486-96. 2011
    ..There was no correlation of Treg frequency with the histologic or clinical severity of gastrointestinal GVHD. We conclude that Treg depletion is not a central feature in the pathogenesis of gastric GVHD in humans...
  34. pmc The preferentially expressed antigen in melanoma (PRAME) inhibits myeloid differentiation in normal hematopoietic and leukemic progenitor cells
    Vivian G Oehler
    Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA, USA
    Blood 114:3299-308. 2009
    ..Lastly, these observations suggest that PRAME is a target for both prognostic and therapeutic applications...
  35. pmc Adoptive immunotherapy for indolent non-Hodgkin lymphoma and mantle cell lymphoma using genetically modified autologous CD20-specific T cells
    Brian G Till
    Clinical Research Division of the Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
    Blood 112:2261-71. 2008
    ..These results show the safety, feasibility, and potential antitumor activity of adoptive T-cell therapy using this approach. This trial was registered at www.clinicaltrials.gov as #NCT00012207...
  36. doi request reprint Evaluation of an 8-color flow cytometric reference method for white blood cell differential enumeration
    Sindhu Cherian
    Department of Laboratory Medicine, University of Washington, Seattle, Washington, USA
    Cytometry B Clin Cytom 78:319-28. 2010
    ..We investigated an 8-color, single-tube, lyse no-wash flow cytometric method to perform an extended 8-part differential as a potential replacement reference method for WBC differential enumeration...
  37. pmc Flow cytometric detection of the classical hodgkin lymphoma: clinical and research applications
    Mikhail Roshal
    Department of Laboratory Medicine, University of Washington, Seattle, WA 98195, USA
    Adv Hematol 2011:387034. 2011
    ..The current state of flow cytometric evaluation of nodular lymphocyte predominant Hodgkin lymphoma and T cell-rich large B cell lymphoma is also briefly discussed...
  38. ncbi request reprint Multicolor immunophenotyping: human immune system hematopoiesis
    Brent Wood
    Department of Laboratory Medicine, University of Washington, Seattle, Washington 98195, USA
    Methods Cell Biol 75:559-76. 2004
  39. pmc Eradication of disseminated leukemia in a syngeneic murine leukemia model using pretargeted anti-CD45 radioimmunotherapy
    John M Pagel
    Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
    Blood 111:2261-8. 2008
    ..These data suggest that anti-CD45 PRIT using an anti-CD45-SA conjugate in a syngeneic murine model of disseminated leukemia may be more effective and less toxic than directly labeled monoclonal antibodies...
  40. pmc Transduction of human NOD/SCID-repopulating cells with both lymphoid and myeloid potential by foamy virus vectors
    Neil C Josephson
    Department of Medicine, Division of Hematology, University of Washington, Seattle, WA 98195, USA
    Proc Natl Acad Sci U S A 99:8295-300. 2002
    ..These findings demonstrate for the first time that human pluripotent (lympho-myeloid) hematopoietic stem cells repopulate NOD/SCID mice and can be efficiently transduced by FV vectors...
  41. ncbi request reprint Productive replication of hepatitis C virus in perihepatic lymph nodes in vivo: implications of HCV lymphotropism
    Sampa Pal
    Department of Laboratory Medicine, University of Washington Medical Center, Seattle, Washington 98104, USA
    Gastroenterology 130:1107-16. 2006
    ..The pathogenesis of chronic hepatitis C is poorly understood. This study examines the ability of hepatitis C virus (HCV) to infect, replicate in, and produce progeny virus from perihepatic lymph nodes in vivo...
  42. ncbi request reprint Pharmacologically regulated in vivo selection in a large animal
    Tobias Neff
    Divisions of Hematology and Oncology, Department of Medicine, University of Washington, Seattle, WA 89195, USA
    Blood 100:2026-31. 2002
    ..Cell growth switches could greatly enhance the efficacy and applicability of gene and cell therapy...
  43. ncbi request reprint Frequent intentional weight loss is associated with lower natural killer cell cytotoxicity in postmenopausal women: possible long-term immune effects
    Erin D Shade
    University of Washington Medical Center, University of Washington, Seattle, WA, USA
    J Am Diet Assoc 104:903-12. 2004
    ..The question of whether this pattern has negative health effects is unresolved. The objective of this research was to investigate associations between weight-loss history and current measures of immune function...
  44. doi request reprint Serum leptin concentrations and markers of immune function in overweight or obese postmenopausal women
    Julie A Meyers
    Department of Epidemiology, University of Washington, Seattle, Washington 98195, USA
    J Endocrinol 199:51-60. 2008
    ..Our study does not support a link between leptin and other immune parameters among overweight or obese, but otherwise healthy postmenopausal women, perhaps because such effects are only present at low or deficient leptin concentrations...