Research Topics
Species | Brent L WoodSummaryAffiliation: University of Washington Country: USA Publications
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Publications
9-color and 10-color flow cytometry in the clinical laboratoryBrent Wood
Department of Laboratory Medicine, University of Washington School of Medicine, Seattle, WA 98195, USA
Arch Pathol Lab Med 130:680-90. 2006..The development of commercial flow cytometers capable of detecting more than 10 simultaneous fluorescent signals presents opportunities for improved diagnosis and monitoring of patients with leukemia and lymphoma...- Four-color flow cytometry shows strong concordance with bone marrow morphology and cytogenetics in the evaluation for myelodysplasiaSteven J Kussick
Department of Laboratory Medicine, University of Washington, Seattle, USA
Am J Clin Pathol 124:170-81. 2005.... - Interactions between mouse IgG2 antibodies are common and mediated by plasma C1qBrent L Wood
Department of Laboratory Medicine, University of Washington Medical Center, Seattle, Washington 98195, USA
Cytometry B Clin Cytom 70:321-8. 2006..The simultaneous use of multiple antibodies for multicolor flow cytometry is increasingly common and presents the opportunity for antibody interactions... - Myeloid malignancies: myelodysplastic syndromes, myeloproliferative disorders, and acute myeloid leukemiaBrent L Wood
Hematopathology Laboratory, Box 357110, Department of Laboratory Medicine, University of Washington Medical Center, 1959 NE Pacific Street, Seattle, WA 98195, USA
Clin Lab Med 27:551-75, vii. 2007..Deviation from this pattern is a hallmark of hematopoietic neoplasia. Application of these principles to myelodysplastic syndromes, myeloproliferative disorders, and acute myeloid leukemia is presented and illustrated... - 2006 Bethesda International Consensus recommendations on the immunophenotypic analysis of hematolymphoid neoplasia by flow cytometry: optimal reagents and reporting for the flow cytometric diagnosis of hematopoietic neoplasiaBrent L Wood
Department of Laboratory Medicine, University of Washington Medical Center, Seattle, Washington 98195, USA
Cytometry B Clin Cytom 72:S14-22. 2007..The 2006 Bethesda Consensus conference represents the first successful attempt to define a set of consensus reagents suitable for the initial evaluation of hematopoietic neoplasia... 
Hematopoietic stem cell expansion facilitates multilineage engraftment in a nonhuman primate cord blood transplantation modelKorashon L Watts
Clinical Research Division, Fred Hutchinson Cancer Research Center FHCRC, Seattle, Wash, USA
Exp Hematol 40:187-96. 2012..Furthermore, it can be used in long-term follow-up studies to determine how specific expansion techniques affect engraftment of various hematopoietic lineages...
Flow cytometric evaluation of skin biopsies for mycosis fungoidesChris H Jokinen
Department of Laboratory Medicine, University of Washington Medical Center, Box 357110 1959 NE Pacific St, Seattle, WA, 91895, USA
Am J Dermatopathol 33:483-91. 2011..The high specificity of FC suggests that it will be a useful adjunct to routine histology in the evaluation of diagnostic skin biopsies for MF...- Anatomical compartments modify the response of human hematopoietic cells to a mitogenic signalYasuo Nagasawa
Department of Medicine, K260 Health Sciences Building, Mailstop 357710, University of Washington, Seattle, Washington 98195, USA
Stem Cells 24:908-17. 2006..These findings establish CIDs as in vivo growth factors for human hematopoietic cells... - Distinct hematopoietic stem/progenitor cell populations are responsible for repopulating NOD/SCID mice compared with nonhuman primatesPeter A Horn
Fred Hutchinson Cancer Research Center, D1-100, PO Box 19024, 1100 Fairview Ave N, Seattle, WA 98109-1024, USA
Blood 102:4329-35. 2003..These results suggest that distinct hematopoietic stem/progenitor cells are responsible for hematopoietic reconstitution in NOD/SCID mice compared with nonhuman primates... - Identification and purification of classical Hodgkin cells from lymph nodes by flow cytometry and flow cytometric cell sortingJonathan R Fromm
Department of Laboratory Medicine, University of Washington, WA 98195, USA
Am J Clin Pathol 126:764-80. 2006..FC may offer an alternative to immunohistochemical analysis in confirming the diagnosis of CHL in certain cases, and, through cell sorting, it provides a means of rapidly isolating pure HRS cells... - Strategies for immunophenotyping and purifying classical Hodgkin lymphoma cells from lymph nodes by flow cytometry and flow cytometric cell sortingJonathan R Fromm
Department of Laboratory Medicine, University of Washington, Seattle 98195, USA
Methods 57:368-75. 2012..This manuscript provides experimental protocols for immunophenotyping CHL by flow cytometry as well as purifying the HRS cells via flow cytometric cell sorting... - Flow cytometric evaluation of CD38 expression assists in distinguishing follicular hyperplasia from follicular lymphomaKristin Mantei
Department of Laboratory Medicine, University of Washington Medical Center, Seattle, Washington 98195, USA
Cytometry B Clin Cytom 76:315-20. 2009..0E-16) in FL in comparison with FH, as has been previously reported. This study identified decreased CD38 as a common finding in FL in comparison with FH and provides an additional tool to help differentiate FL from FH by flow cytometry... - Four-color flow cytometry identifies virtually all cytogenetically abnormal bone marrow samples in the workup of non-CML myeloproliferative disordersSteven J Kussick
Department of Laboratory Medicine, University of Washington, Seattle, USA
Am J Clin Pathol 120:854-65. 2003..In experienced laboratories, 4-color flow cytometry represents a useful method to help distinguish benign from neoplastic marrow in the workup of non-CML MPDs... - Using 4-color flow cytometry to identify abnormal myeloid populationsSteven J Kussick
University of Washington Department of Laboratory Medicine, Seattle, Wash, USA
Arch Pathol Lab Med 127:1140-7. 2003.... - Flow cytometry can diagnose classical hodgkin lymphoma in lymph nodes with high sensitivity and specificityJonathan R Fromm
Department of Laboratory Medicine, University of Washington, Seattle, WA 98195, USA
Am J Clin Pathol 131:322-32. 2009..With the current availability of 8 (or more) color clinical flow cytometers, this assay can now be applied to routinely immunophenotype and confirm a diagnosis of CHL or as an adjunct to immunohistochemical analysis... - High-throughput sequencing detects minimal residual disease in acute T lymphoblastic leukemiaDavid Wu
Department of Laboratory Medicine, University of Washington, Seattle, WA 98109, USA
Sci Transl Med 4:134ra63. 2012..Thus, next-generation sequencing of lymphoid receptor gene repertoire may improve clinical diagnosis and subsequent MRD monitoring of lymphoproliferative disorders... - Ten-color immunophenotyping of hematopoietic cellsBrent L Wood
University of Washington, Seattle, Washington, USA
Curr Protoc Cytom . 2005.... - "Double-Hit" mature B-cell lymphomas show a common immunophenotype by flow cytometry that includes decreased CD20 expressionDavid Wu
University of Washington Medical Center, Seattle Cancer Care Alliance, Seattle, WA 98109, USA
Am J Clin Pathol 134:258-65. 2010.... - Lineage-specific identification of nonhematopoietic neoplasms by flow cytometryAnthony Chang
University of Washington, Department of Laboratory Medicine, 1959 NE Pacific St, Seattle, WA 98195, USA
Am J Clin Pathol 119:643-55. 2003.... - Immaturity associated antigens are lost during induction for T cell lymphoblastic leukemia: implications for minimal residual disease detectionMikhail Roshal
Department of Laboratory Medicine, University of Washington Seattle, Washington, USA
Cytometry B Clin Cytom 78:139-46. 2010..Little information is available about the stability of these antigens in precursor T cell ALL (T-ALL) though it is presumed that CD99 and terminal deoxynucleotidyl transferase (TdT) are highly informative based on limited studies... - Prominent clonal B-cell populations identified by flow cytometry in histologically reactive lymphoid proliferationsSteven J Kussick
Department of Laboratory Medicine, University of Washington, Seattle, USA
Am J Clin Pathol 121:464-72. 2004..Our findings add rare cases of follicular hyperplasia to the list of histologically reactive settings in which clonal B-cell populations might be present... - Impact of pretransplantation minimal residual disease, as detected by multiparametric flow cytometry, on outcome of myeloablative hematopoietic cell transplantation for acute myeloid leukemiaRoland B Walter
Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109 1024, USA
J Clin Oncol 29:1190-7. 2011..Hitherto, little attention has been given to the prognostic impact of pretransplantation minimal residual disease (MRD)... - High incidence of leukemia in large animals after stem cell gene therapy with a HOXB4-expressing retroviral vectorXiao Bing Zhang
Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109 1024, USA
J Clin Invest 118:1502-10. 2008..Our data thus suggest the continued need for caution in genetic manipulation of repopulating cells, particularly when the transgene might impart an intrinsic growth advantage... - Decreased NK cell frequency in chronic hepatitis C does not affect ex vivo cytolytic killingChihiro Morishima
Department of Pediatrics, University of Washington School of Medicine, Seattle, WA, USA
Hepatology 43:573-80. 2006..035). In conclusion, NK cell cytolytic function does not appear to be impaired in chronic hepatitis C, but higher levels of NK cell cytolysis are associated with less liver fibrosis... - High-dose chemo-radioimmunotherapy with autologous stem cell support for relapsed mantle cell lymphomaAjay K Gopal
Clinical Research Division, Fred Hutchinson Cancer Research Center, University of Washington, Seattle, WA, USA
Blood 99:3158-62. 2002..High-dose treatment with (131)I-Tositumomab, etoposide, and cyclophosphamide results in a high remission rate and may provide long-term disease-free survival for patients with relapsed or refractory mantle cell lymphoma... - Augmented mobilization and collection of CD34+ hematopoietic cells from normal human volunteers stimulated with granulocyte-colony-stimulating factor by single-dose administration of AMD3100, a CXCR4 antagonistW Conrad Liles
Department of Medicine, University of Washington, Seattle, Washington 98195, USA
Transfusion 45:295-300. 2005..CONCLUSION: These findings indicate that AMD3100 can be used alone or as an adjunct to G-CSF to mobilize cells for HPC transplantation... - Mobilization of hematopoietic progenitor cells in healthy volunteers by AMD3100, a CXCR4 antagonistW Conrad Liles
Department of Medicine, University of Washington, HSB AA 522, Box 356422, Seattle, WA 98195
Blood 102:2728-30. 2003..AMD3100 was well tolerated and caused only mild, transient toxicity. These findings suggest potential clinical application of AMD3100 for CD34+ cell mobilization and collection for hematopoietic stem cell transplantation... - Blood and gastric FOXP3+ T cells are not decreased in human gastric graft-versus-host diseaseJames D Lord
Department of Medicine, University of Washington School of Medicine, Seattle, WA 98101, USA
Biol Blood Marrow Transplant 17:486-96. 2011..There was no correlation of Treg frequency with the histologic or clinical severity of gastrointestinal GVHD. We conclude that Treg depletion is not a central feature in the pathogenesis of gastric GVHD in humans... - The preferentially expressed antigen in melanoma (PRAME) inhibits myeloid differentiation in normal hematopoietic and leukemic progenitor cellsVivian G Oehler
Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA, USA
Blood 114:3299-308. 2009..Lastly, these observations suggest that PRAME is a target for both prognostic and therapeutic applications... - Lymphomatoid papulosis with CD1a+ dendritic cell hyperplasia, mimicking Langerhans cell histiocytosisChris H Jokinen
Department of Pathology, University of Washington, Seattle, WA 98195, USA
J Cutan Pathol 34:584-7. 2007.... - Flow cytometry for non-Hodgkin and classical Hodgkin lymphomaDavid Wu
Department of Laboratory Medicine, University of Washington, Seattle, WA, USA
Methods Mol Biol 971:27-47. 2013..We highlight an overview of procedures for processing and immunophenotyping non-Hodgkin B- and T-cell lymphomas and also describe our strategy for the sensitive and specific diagnosis of classical Hodgkin lymphoma... - Evaluation of an 8-color flow cytometric reference method for white blood cell differential enumerationSindhu Cherian
Department of Laboratory Medicine, University of Washington, Seattle, Washington, USA
Cytometry B Clin Cytom 78:319-28. 2010..We investigated an 8-color, single-tube, lyse no-wash flow cytometric method to perform an extended 8-part differential as a potential replacement reference method for WBC differential enumeration... - Adoptive immunotherapy for indolent non-Hodgkin lymphoma and mantle cell lymphoma using genetically modified autologous CD20-specific T cellsBrian G Till
Clinical Research Division of the Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
Blood 112:2261-71. 2008..These results show the safety, feasibility, and potential antitumor activity of adoptive T-cell therapy using this approach. This trial was registered at www.clinicaltrials.gov as #NCT00012207... - Flow cytometric detection of the classical hodgkin lymphoma: clinical and research applicationsMikhail Roshal
Department of Laboratory Medicine, University of Washington, Seattle, WA 98195, USA
Adv Hematol 2011:387034. 2011..The current state of flow cytometric evaluation of nodular lymphocyte predominant Hodgkin lymphoma and T cell-rich large B cell lymphoma is also briefly discussed... - Multicolor immunophenotyping: human immune system hematopoiesisBrent Wood
Department of Laboratory Medicine, University of Washington, Seattle, Washington 98195, USA
Methods Cell Biol 75:559-76. 2004 - Productive replication of hepatitis C virus in perihepatic lymph nodes in vivo: implications of HCV lymphotropismSampa Pal
Department of Laboratory Medicine, University of Washington Medical Center, Seattle, Washington 98104, USA
Gastroenterology 130:1107-16. 2006..We demonstrate for the first time a major contribution of extrahepatic HCV replication to circulating virus in serum (viremia)... - Eradication of disseminated leukemia in a syngeneic murine leukemia model using pretargeted anti-CD45 radioimmunotherapyJohn M Pagel
Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
Blood 111:2261-8. 2008..These data suggest that anti-CD45 PRIT using an anti-CD45-SA conjugate in a syngeneic murine model of disseminated leukemia may be more effective and less toxic than directly labeled monoclonal antibodies... - Transduction of human NOD/SCID-repopulating cells with both lymphoid and myeloid potential by foamy virus vectorsNeil C Josephson
Department of Medicine, Division of Hematology, University of Washington, Seattle, WA 98195, USA
Proc Natl Acad Sci U S A 99:8295-300. 2002..These findings demonstrate for the first time that human pluripotent (lympho-myeloid) hematopoietic stem cells repopulate NOD/SCID mice and can be efficiently transduced by FV vectors... - Pharmacologically regulated in vivo selection in a large animalTobias Neff
Divisions of Hematology and Oncology, Department of Medicine, University of Washington, Seattle, WA 89195, USA
Blood 100:2026-31. 2002..Cell growth switches could greatly enhance the efficacy and applicability of gene and cell therapy... - Frequent intentional weight loss is associated with lower natural killer cell cytotoxicity in postmenopausal women: possible long-term immune effectsErin D Shade
University of Washington Medical Center, University of Washington, Seattle, WA, USA
J Am Diet Assoc 104:903-12. 2004..CONCLUSIONS: This study provides evidence that frequent intentional weight loss may have long-term effects on immune function... - Serum leptin concentrations and markers of immune function in overweight or obese postmenopausal womenJulie A Meyers
Department of Epidemiology, University of Washington, Seattle, Washington 98195, USA
J Endocrinol 199:51-60. 2008..Our study does not support a link between leptin and other immune parameters among overweight or obese, but otherwise healthy postmenopausal women, perhaps because such effects are only present at low or deficient leptin concentrations...