Donna M Wolk

Summary

Affiliation: University of Arizona
Country: USA

Publications

  1. ncbi PCR-electrospray ionization mass spectrometry: the potential to change infectious disease diagnostics in clinical and public health laboratories
    Donna M Wolk
    Division of Clinical Microbiology, Department of Pathology BIO5 Institute, University of Arizona, Tucson, AZ 85724, USA
    J Mol Diagn 14:295-304. 2012
  2. ncbi Multicenter evaluation of the Cepheid Xpert methicillin-resistant Staphylococcus aureus (MRSA) test as a rapid screening method for detection of MRSA in nares
    D M Wolk
    The University of Arizona BIO5 Institute, Tucson, Arizona 85724 5059, USA
    J Clin Microbiol 47:758-64. 2009
  3. ncbi Rapid detection of Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) in wound specimens and blood cultures: multicenter preclinical evaluation of the Cepheid Xpert MRSA/SA skin and soft tissue and blood culture assays
    D M Wolk
    University of Arizona BIO5 Institute, Tucson, Arizona 85724 5059, USA
    J Clin Microbiol 47:823-6. 2009
  4. ncbi Pathogen profiling: rapid molecular characterization of Staphylococcus aureus by PCR/electrospray ionization-mass spectrometry and correlation with phenotype
    Donna M Wolk
    University of Arizona, BIO5 Institute, Department of Pathology, 1501 N Campbell Ave, Tucson, AZ 85724 5059, USA
    J Clin Microbiol 47:3129-37. 2009
  5. ncbi Comparison of MRSASelect Agar, CHROMagar Methicillin-Resistant Staphylococcus aureus (MRSA) Medium, and Xpert MRSA PCR for detection of MRSA in Nares: diagnostic accuracy for surveillance samples with various bacterial densities
    D M Wolk
    Southern Arizona VA Health Care System, Tucson, Arizona, USA
    J Clin Microbiol 47:3933-6. 2009
  6. ncbi Comparative analysis of PCR-electrospray ionization/mass spectrometry (MS) and MALDI-TOF/MS for the identification of bacteria and yeast from positive blood culture bottles
    Erin J Kaleta
    Department of Chemistry and Biochemistry, University of Arizona, Tucson, Arizona, USA
    Clin Chem 57:1057-67. 2011
  7. ncbi Use of PCR coupled with electrospray ionization mass spectrometry for rapid identification of bacterial and yeast bloodstream pathogens from blood culture bottles
    Erin J Kaleta
    Department of Chemistry and Biochemistry, University of Arizona, Tucson, Arizona 85724 5059, USA
    J Clin Microbiol 49:345-53. 2011
  8. ncbi Diagnostic accuracy of the Cepheid GeneXpert vanA/vanB assay ver. 1.0 to detect the vanA and vanB vancomycin resistance genes in Enterococcus from perianal specimens
    Erin S Marner
    University of Arizona, Tucson, AZ 85724 5059, USA
    Diagn Microbiol Infect Dis 69:382-9. 2011
  9. ncbi Diabetic foot infections: A need for innovative assessments
    Timothy K Fisher
    University of Arizona, Tucson, 85724, USA
    Int J Low Extrem Wounds 9:31-6. 2010
  10. ncbi Laboratory diagnosis of viral hepatitis
    D M Wolk
    Molecular Diagnostic Laboratories, Southern Arizona VA Health Care System, Department of Pathology, University of Arizona, Tucson, Arizona, USA
    Infect Dis Clin North Am 15:1109-26. 2001

Collaborators

Detail Information

Publications10

  1. ncbi PCR-electrospray ionization mass spectrometry: the potential to change infectious disease diagnostics in clinical and public health laboratories
    Donna M Wolk
    Division of Clinical Microbiology, Department of Pathology BIO5 Institute, University of Arizona, Tucson, AZ 85724, USA
    J Mol Diagn 14:295-304. 2012
    ..This review considers the breadth of the published literature and explores the possibilities, advantages, and limitations for implementation of PCR-ESI/MS in diagnostic laboratories...
  2. ncbi Multicenter evaluation of the Cepheid Xpert methicillin-resistant Staphylococcus aureus (MRSA) test as a rapid screening method for detection of MRSA in nares
    D M Wolk
    The University of Arizona BIO5 Institute, Tucson, Arizona 85724 5059, USA
    J Clin Microbiol 47:758-64. 2009
    ..From this large-scale, multicenter clinical comparison, we conclude that the Xpert MRSA assay is a simple, rapid, and accurate method for performing active surveillance for MRSA in a variety of health care populations...
  3. ncbi Rapid detection of Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) in wound specimens and blood cultures: multicenter preclinical evaluation of the Cepheid Xpert MRSA/SA skin and soft tissue and blood culture assays
    D M Wolk
    University of Arizona BIO5 Institute, Tucson, Arizona 85724 5059, USA
    J Clin Microbiol 47:823-6. 2009
    ..aureus. Sensitivity was 97.1% and 98.3% for MRSA in wound and blood culture specimens, respectively. Sensitivity was 100% for S. aureus from both specimen types...
  4. ncbi Pathogen profiling: rapid molecular characterization of Staphylococcus aureus by PCR/electrospray ionization-mass spectrometry and correlation with phenotype
    Donna M Wolk
    University of Arizona, BIO5 Institute, Department of Pathology, 1501 N Campbell Ave, Tucson, AZ 85724 5059, USA
    J Clin Microbiol 47:3129-37. 2009
    ..The high-throughput PCR/ESI-MS assay should improve clinical management of staphylococcal infections...
  5. ncbi Comparison of MRSASelect Agar, CHROMagar Methicillin-Resistant Staphylococcus aureus (MRSA) Medium, and Xpert MRSA PCR for detection of MRSA in Nares: diagnostic accuracy for surveillance samples with various bacterial densities
    D M Wolk
    Southern Arizona VA Health Care System, Tucson, Arizona, USA
    J Clin Microbiol 47:3933-6. 2009
    ..In this active-surveillance convenience sample, the use of broth enrichment followed by subculture to MS offered a low-cost but sensitive method for MRSA screening, with performance similar to that of Xpert MRSA PCR...
  6. ncbi Comparative analysis of PCR-electrospray ionization/mass spectrometry (MS) and MALDI-TOF/MS for the identification of bacteria and yeast from positive blood culture bottles
    Erin J Kaleta
    Department of Chemistry and Biochemistry, University of Arizona, Tucson, Arizona, USA
    Clin Chem 57:1057-67. 2011
    ..A positive challenge set of blood culture bottles was used to compare PCR-ESI/MS and MALDI-TOF/MS performance on a matched set of samples...
  7. ncbi Use of PCR coupled with electrospray ionization mass spectrometry for rapid identification of bacterial and yeast bloodstream pathogens from blood culture bottles
    Erin J Kaleta
    Department of Chemistry and Biochemistry, University of Arizona, Tucson, Arizona 85724 5059, USA
    J Clin Microbiol 49:345-53. 2011
    ....
  8. ncbi Diagnostic accuracy of the Cepheid GeneXpert vanA/vanB assay ver. 1.0 to detect the vanA and vanB vancomycin resistance genes in Enterococcus from perianal specimens
    Erin S Marner
    University of Arizona, Tucson, AZ 85724 5059, USA
    Diagn Microbiol Infect Dis 69:382-9. 2011
    ..In summary, the GeneXpert vanA/vanB assay is a rapid and accurate method to identify vanA/vanB-colonized patients for VRE screening programs that use perianal swab specimens...
  9. ncbi Diabetic foot infections: A need for innovative assessments
    Timothy K Fisher
    University of Arizona, Tucson, 85724, USA
    Int J Low Extrem Wounds 9:31-6. 2010
    ..Adopting these advances in diagnosis and treatment may help with overall prognosis and reduce health care costs...
  10. ncbi Laboratory diagnosis of viral hepatitis
    D M Wolk
    Molecular Diagnostic Laboratories, Southern Arizona VA Health Care System, Department of Pathology, University of Arizona, Tucson, Arizona, USA
    Infect Dis Clin North Am 15:1109-26. 2001
    ..Both qualitative and quantitative molecular assays are available, and their efficient use requires familiarity with the sensitivity and dynamic ranges of each method...