Donna M Wolk

Summary

Affiliation: University of Arizona
Country: USA

Publications

  1. doi request reprint PCR-electrospray ionization mass spectrometry: the potential to change infectious disease diagnostics in clinical and public health laboratories
    Donna M Wolk
    Division of Clinical Microbiology, Department of Pathology BIO5 Institute, University of Arizona, Tucson, AZ 85724, USA
    J Mol Diagn 14:295-304. 2012
  2. pmc Multicenter evaluation of the Cepheid Xpert methicillin-resistant Staphylococcus aureus (MRSA) test as a rapid screening method for detection of MRSA in nares
    D M Wolk
    The University of Arizona BIO5 Institute, Tucson, Arizona 85724 5059, USA
    J Clin Microbiol 47:758-64. 2009
  3. pmc Rapid detection of Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) in wound specimens and blood cultures: multicenter preclinical evaluation of the Cepheid Xpert MRSA/SA skin and soft tissue and blood culture assays
    D M Wolk
    University of Arizona BIO5 Institute, Tucson, Arizona 85724 5059, USA
    J Clin Microbiol 47:823-6. 2009
  4. pmc Pathogen profiling: rapid molecular characterization of Staphylococcus aureus by PCR/electrospray ionization-mass spectrometry and correlation with phenotype
    Donna M Wolk
    University of Arizona, BIO5 Institute, Department of Pathology, 1501 N Campbell Ave, Tucson, AZ 85724 5059, USA
    J Clin Microbiol 47:3129-37. 2009
  5. pmc Comparison of MRSASelect Agar, CHROMagar Methicillin-Resistant Staphylococcus aureus (MRSA) Medium, and Xpert MRSA PCR for detection of MRSA in Nares: diagnostic accuracy for surveillance samples with various bacterial densities
    D M Wolk
    Southern Arizona VA Health Care System, Tucson, Arizona, USA
    J Clin Microbiol 47:3933-6. 2009
  6. doi request reprint Comparative analysis of PCR-electrospray ionization/mass spectrometry (MS) and MALDI-TOF/MS for the identification of bacteria and yeast from positive blood culture bottles
    Erin J Kaleta
    Department of Chemistry and Biochemistry, University of Arizona, Tucson, Arizona, USA
    Clin Chem 57:1057-67. 2011
  7. pmc Use of PCR coupled with electrospray ionization mass spectrometry for rapid identification of bacterial and yeast bloodstream pathogens from blood culture bottles
    Erin J Kaleta
    Department of Chemistry and Biochemistry, University of Arizona, Tucson, Arizona 85724 5059, USA
    J Clin Microbiol 49:345-53. 2011
  8. doi request reprint Diagnostic accuracy of the Cepheid GeneXpert vanA/vanB assay ver. 1.0 to detect the vanA and vanB vancomycin resistance genes in Enterococcus from perianal specimens
    Erin S Marner
    University of Arizona, Tucson, AZ 85724 5059, USA
    Diagn Microbiol Infect Dis 69:382-9. 2011
  9. pmc Matrix-assisted laser desorption ionization-time of flight mass spectrometry: a fundamental shift in the routine practice of clinical microbiology
    Andrew E Clark
    Department of Veterinary Science and Microbiology, University of Arizona, Tucson, Arizona, USA
    Clin Microbiol Rev 26:547-603. 2013
  10. doi request reprint Would earlier microbe identification alter antibiotic therapy in bacteremic emergency department patients?
    Lisa R Stoneking
    Department of Emergency Medicine, University of Arizona, Tucson, Arizona 85724, USA
    J Emerg Med 44:1-8. 2013

Collaborators

Detail Information

Publications12

  1. doi request reprint PCR-electrospray ionization mass spectrometry: the potential to change infectious disease diagnostics in clinical and public health laboratories
    Donna M Wolk
    Division of Clinical Microbiology, Department of Pathology BIO5 Institute, University of Arizona, Tucson, AZ 85724, USA
    J Mol Diagn 14:295-304. 2012
    ..This review considers the breadth of the published literature and explores the possibilities, advantages, and limitations for implementation of PCR-ESI/MS in diagnostic laboratories...
  2. pmc Multicenter evaluation of the Cepheid Xpert methicillin-resistant Staphylococcus aureus (MRSA) test as a rapid screening method for detection of MRSA in nares
    D M Wolk
    The University of Arizona BIO5 Institute, Tucson, Arizona 85724 5059, USA
    J Clin Microbiol 47:758-64. 2009
    ..From this large-scale, multicenter clinical comparison, we conclude that the Xpert MRSA assay is a simple, rapid, and accurate method for performing active surveillance for MRSA in a variety of health care populations...
  3. pmc Rapid detection of Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) in wound specimens and blood cultures: multicenter preclinical evaluation of the Cepheid Xpert MRSA/SA skin and soft tissue and blood culture assays
    D M Wolk
    University of Arizona BIO5 Institute, Tucson, Arizona 85724 5059, USA
    J Clin Microbiol 47:823-6. 2009
    ..aureus. Sensitivity was 97.1% and 98.3% for MRSA in wound and blood culture specimens, respectively. Sensitivity was 100% for S. aureus from both specimen types...
  4. pmc Pathogen profiling: rapid molecular characterization of Staphylococcus aureus by PCR/electrospray ionization-mass spectrometry and correlation with phenotype
    Donna M Wolk
    University of Arizona, BIO5 Institute, Department of Pathology, 1501 N Campbell Ave, Tucson, AZ 85724 5059, USA
    J Clin Microbiol 47:3129-37. 2009
    ..The high-throughput PCR/ESI-MS assay should improve clinical management of staphylococcal infections...
  5. pmc Comparison of MRSASelect Agar, CHROMagar Methicillin-Resistant Staphylococcus aureus (MRSA) Medium, and Xpert MRSA PCR for detection of MRSA in Nares: diagnostic accuracy for surveillance samples with various bacterial densities
    D M Wolk
    Southern Arizona VA Health Care System, Tucson, Arizona, USA
    J Clin Microbiol 47:3933-6. 2009
    ..In this active-surveillance convenience sample, the use of broth enrichment followed by subculture to MS offered a low-cost but sensitive method for MRSA screening, with performance similar to that of Xpert MRSA PCR...
  6. doi request reprint Comparative analysis of PCR-electrospray ionization/mass spectrometry (MS) and MALDI-TOF/MS for the identification of bacteria and yeast from positive blood culture bottles
    Erin J Kaleta
    Department of Chemistry and Biochemistry, University of Arizona, Tucson, Arizona, USA
    Clin Chem 57:1057-67. 2011
    ..A positive challenge set of blood culture bottles was used to compare PCR-ESI/MS and MALDI-TOF/MS performance on a matched set of samples...
  7. pmc Use of PCR coupled with electrospray ionization mass spectrometry for rapid identification of bacterial and yeast bloodstream pathogens from blood culture bottles
    Erin J Kaleta
    Department of Chemistry and Biochemistry, University of Arizona, Tucson, Arizona 85724 5059, USA
    J Clin Microbiol 49:345-53. 2011
    ....
  8. doi request reprint Diagnostic accuracy of the Cepheid GeneXpert vanA/vanB assay ver. 1.0 to detect the vanA and vanB vancomycin resistance genes in Enterococcus from perianal specimens
    Erin S Marner
    University of Arizona, Tucson, AZ 85724 5059, USA
    Diagn Microbiol Infect Dis 69:382-9. 2011
    ..In summary, the GeneXpert vanA/vanB assay is a rapid and accurate method to identify vanA/vanB-colonized patients for VRE screening programs that use perianal swab specimens...
  9. pmc Matrix-assisted laser desorption ionization-time of flight mass spectrometry: a fundamental shift in the routine practice of clinical microbiology
    Andrew E Clark
    Department of Veterinary Science and Microbiology, University of Arizona, Tucson, Arizona, USA
    Clin Microbiol Rev 26:547-603. 2013
    ..Finally, this review closes with a prospective view of the future of MALDI-TOF MS in the clinical microbiology laboratory to accelerate diagnosis and microbial identification to improve patient care...
  10. doi request reprint Would earlier microbe identification alter antibiotic therapy in bacteremic emergency department patients?
    Lisa R Stoneking
    Department of Emergency Medicine, University of Arizona, Tucson, Arizona 85724, USA
    J Emerg Med 44:1-8. 2013
    ..Although debate exists about the treatment of sepsis, few disagree about the benefits of early, appropriately targeted antibiotic administration...
  11. doi request reprint Diabetic foot infections: A need for innovative assessments
    Timothy K Fisher
    University of Arizona, Tucson, 85724, USA
    Int J Low Extrem Wounds 9:31-6. 2010
    ..Adopting these advances in diagnosis and treatment may help with overall prognosis and reduce health care costs...
  12. ncbi request reprint Laboratory diagnosis of viral hepatitis
    D M Wolk
    Molecular Diagnostic Laboratories, Southern Arizona VA Health Care System, Department of Pathology, University of Arizona, Tucson, Arizona, USA
    Infect Dis Clin North Am 15:1109-26. 2001
    ..Both qualitative and quantitative molecular assays are available, and their efficient use requires familiarity with the sensitivity and dynamic ranges of each method...