C T Wittwer

Summary

Affiliation: University of Utah
Country: USA

Publications

  1. ncbi Continuous fluorescence monitoring of rapid cycle DNA amplification
    C T Wittwer
    Department of Pathology, University of Utah Medical School, Salt Lake City 84132, USA
    Biotechniques 22:130-1, 134-8. 1997
  2. ncbi The LightCycler: a microvolume multisample fluorimeter with rapid temperature control
    C T Wittwer
    Idaho Technology, Idaho Falls, USA
    Biotechniques 22:176-81. 1997
  3. ncbi Quantification of HER2/neu gene amplification by competitive pcr using fluorescent melting curve analysis
    E Lyon
    Department of Pathology, School of Medicine, University of Utah, Salt Lake 84132, USA
    Clin Chem 47:844-51. 2001
  4. ncbi Real-time multiplex PCR assays
    C T Wittwer
    Department of Pathology, University of Utah School of Medicine, Salt Lake City, Utah 84132, USA
    Methods 25:430-42. 2001
  5. ncbi Quantification of low-copy transcripts by continuous SYBR Green I monitoring during amplification
    T B Morrison
    Department of Pathology, University of Utah Medical School, Salt Lake City 84132, USA
    Biotechniques 24:954-8, 960, 962. 1998
  6. ncbi High-resolution DNA melting curve analysis to establish HLA genotypic identity
    L Zhou
    Department of Pathology, University of Utah, Salt Lake City, 84132, USA
    Tissue Antigens 64:156-64. 2004

Detail Information

Publications6

  1. ncbi Continuous fluorescence monitoring of rapid cycle DNA amplification
    C T Wittwer
    Department of Pathology, University of Utah Medical School, Salt Lake City 84132, USA
    Biotechniques 22:130-1, 134-8. 1997
    ..Continuous within-cycle monitoring allows rapid optimization of amplification conditions and should be particularly useful in developing new, standardized clinical assays...
  2. ncbi The LightCycler: a microvolume multisample fluorimeter with rapid temperature control
    C T Wittwer
    Idaho Technology, Idaho Falls, USA
    Biotechniques 22:176-81. 1997
    ..Complete amplification and analysis requires only 10-15 min...
  3. ncbi Quantification of HER2/neu gene amplification by competitive pcr using fluorescent melting curve analysis
    E Lyon
    Department of Pathology, School of Medicine, University of Utah, Salt Lake 84132, USA
    Clin Chem 47:844-51. 2001
    ..We designed a quantitative PCR system utilizing fluorescent hybridization probes and a competitor that differed from the HER2/neu sequence by a single base change...
  4. ncbi Real-time multiplex PCR assays
    C T Wittwer
    Department of Pathology, University of Utah School of Medicine, Salt Lake City, Utah 84132, USA
    Methods 25:430-42. 2001
    ..Instead of physical separation along the X and Y axes, amplification products are identified by different fluorescence spectra and melting characteristics...
  5. ncbi Quantification of low-copy transcripts by continuous SYBR Green I monitoring during amplification
    T B Morrison
    Department of Pathology, University of Utah Medical School, Salt Lake City 84132, USA
    Biotechniques 24:954-8, 960, 962. 1998
    ..Above 10 copies per reaction, typical replicate coefficients of variation were 6%-37%, with better precision at higher copy numbers...
  6. ncbi High-resolution DNA melting curve analysis to establish HLA genotypic identity
    L Zhou
    Department of Pathology, University of Utah, Salt Lake City, 84132, USA
    Tissue Antigens 64:156-64. 2004
    ..The technique may also prove useful for confirmation of HLA genotypic identity between unrelated individuals prior to allogeneic hematopoietic stem-cell transplantation...