C T Wittwer

Summary

Affiliation: University of Utah
Country: USA

Publications

  1. ncbi request reprint High-resolution genotyping by amplicon melting analysis using LCGreen
    Carl T Wittwer
    Department of Pathology, University of Utah Medical School, Salt Lake City, UT 84132, USA
    Clin Chem 49:853-60. 2003
  2. doi request reprint High-resolution DNA melting analysis: advancements and limitations
    Carl T Wittwer
    Department of Pathology, University of Utah School of Medicine, Salt Lake City, UT 84132, USA
    Hum Mutat 30:857-9. 2009
  3. ncbi request reprint Real-time multiplex PCR assays
    C T Wittwer
    Department of Pathology, University of Utah School of Medicine, Salt Lake City, Utah 84132, USA
    Methods 25:430-42. 2001
  4. pmc High resolution melting analysis for gene scanning
    Maria Erali
    ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, UT 84108, USA
    Methods 50:250-61. 2010
  5. ncbi request reprint Amplicon melting analysis with labeled primers: a closed-tube method for differentiating homozygotes and heterozygotes
    Cameron N Gundry
    Department of Pathology, University of Utah Medical School, Salt Lake City, UT 84132, USA
    Clin Chem 49:396-406. 2003
  6. ncbi request reprint Unlabeled oligonucleotide probes modified with locked nucleic acids for improved mismatch discrimination in genotyping by melting analysis
    Lan Szu Chou
    ARUP Laboratories, Salt Lake City, UT 84108 1221, USA
    Biotechniques 39:644, 646, 648 passim. 2005
  7. ncbi request reprint High-resolution DNA melting analysis for simultaneous mutation scanning and genotyping in solution
    Luming Zhou
    Department of Pathology, University of Utah Medical Center, Salt Lake City, UT 84105, USA
    Clin Chem 51:1770-7. 2005
  8. ncbi request reprint Continuous fluorescence monitoring of rapid cycle DNA amplification
    C T Wittwer
    Department of Pathology, University of Utah Medical School, Salt Lake City 84132, USA
    Biotechniques 22:130-1, 134-8. 1997
  9. ncbi request reprint Scanning the cystic fibrosis transmembrane conductance regulator gene using high-resolution DNA melting analysis
    Jesse Montgomery
    Department of Pathology, University of Utah Health Sciences Center, Salt Lake City, UT 84132, USA
    Clin Chem 53:1891-8. 2007
  10. pmc Characterization of aberrant melting peaks in unlabeled probe assays
    Shale Dames
    ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, UT 84108, USA
    J Mol Diagn 9:290-6. 2007

Research Grants

  1. HOMOGENEOUS MULTIPLEX PCR BY FLUORESCENCE AND TM
    Carl Wittwer; Fiscal Year: 2002
  2. Homogeneous Mutation Scanning
    Carl Wittwer; Fiscal Year: 2006
  3. A System for Rapid PCR, Mutation Scanning and Genotyping
    Carl Wittwer; Fiscal Year: 2006

Collaborators

Detail Information

Publications64

  1. ncbi request reprint High-resolution genotyping by amplicon melting analysis using LCGreen
    Carl T Wittwer
    Department of Pathology, University of Utah Medical School, Salt Lake City, UT 84132, USA
    Clin Chem 49:853-60. 2003
    ..Our aim was to develop a closed-tube system for genotyping and mutation scanning that did not require labeled oligonucleotides...
  2. doi request reprint High-resolution DNA melting analysis: advancements and limitations
    Carl T Wittwer
    Department of Pathology, University of Utah School of Medicine, Salt Lake City, UT 84132, USA
    Hum Mutat 30:857-9. 2009
    ....
  3. ncbi request reprint Real-time multiplex PCR assays
    C T Wittwer
    Department of Pathology, University of Utah School of Medicine, Salt Lake City, Utah 84132, USA
    Methods 25:430-42. 2001
    ..Instead of physical separation along the X and Y axes, amplification products are identified by different fluorescence spectra and melting characteristics...
  4. pmc High resolution melting analysis for gene scanning
    Maria Erali
    ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, UT 84108, USA
    Methods 50:250-61. 2010
    ..For each step in the protocol, a general overview of principles is provided, followed by an in depth analysis of one example, scanning of CYBB, the gene that is mutated in X-linked chronic granulomatous disease...
  5. ncbi request reprint Amplicon melting analysis with labeled primers: a closed-tube method for differentiating homozygotes and heterozygotes
    Cameron N Gundry
    Department of Pathology, University of Utah Medical School, Salt Lake City, UT 84132, USA
    Clin Chem 49:396-406. 2003
    ..Common methods for identification of DNA sequence variants use gel electrophoresis or column separation after PCR...
  6. ncbi request reprint Unlabeled oligonucleotide probes modified with locked nucleic acids for improved mismatch discrimination in genotyping by melting analysis
    Lan Szu Chou
    ARUP Laboratories, Salt Lake City, UT 84108 1221, USA
    Biotechniques 39:644, 646, 648 passim. 2005
  7. ncbi request reprint High-resolution DNA melting analysis for simultaneous mutation scanning and genotyping in solution
    Luming Zhou
    Department of Pathology, University of Utah Medical Center, Salt Lake City, UT 84105, USA
    Clin Chem 51:1770-7. 2005
    ..However, simultaneous PCR product scanning and probe genotyping in the same reaction has not been described...
  8. ncbi request reprint Continuous fluorescence monitoring of rapid cycle DNA amplification
    C T Wittwer
    Department of Pathology, University of Utah Medical School, Salt Lake City 84132, USA
    Biotechniques 22:130-1, 134-8. 1997
    ..Continuous within-cycle monitoring allows rapid optimization of amplification conditions and should be particularly useful in developing new, standardized clinical assays...
  9. ncbi request reprint Scanning the cystic fibrosis transmembrane conductance regulator gene using high-resolution DNA melting analysis
    Jesse Montgomery
    Department of Pathology, University of Utah Health Sciences Center, Salt Lake City, UT 84132, USA
    Clin Chem 53:1891-8. 2007
    ..High-resolution DNA melting analysis is a rapid, closed-tube alternative for gene scanning and genotyping...
  10. pmc Characterization of aberrant melting peaks in unlabeled probe assays
    Shale Dames
    ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, UT 84108, USA
    J Mol Diagn 9:290-6. 2007
    ....
  11. ncbi request reprint Amplicon DNA melting analysis for mutation scanning and genotyping: cross-platform comparison of instruments and dyes
    Mark G Herrmann
    Institute for Clinical and Experimental Pathology, ARUP, Salt Lake City, UT, USA
    Clin Chem 52:494-503. 2006
    ..The comparative performance of other instruments and dyes has not been evaluated...
  12. pmc Base-pair neutral homozygotes can be discriminated by calibrated high-resolution melting of small amplicons
    Cameron N Gundry
    Idaho Technology Inc, 390 Wakara Way and Department of Pathology, University of Utah School of Medicine, 50 North Medical Drive 5B426, Salt Lake City, Utah 84108, USA
    Nucleic Acids Res 36:3401-8. 2008
    ..Unexpectedly, we were able to distinguish these homozygotes by T(m) even though current nearest neighbor models predict that the two homozygous alleles would be identical...
  13. ncbi request reprint The LightCycler: a microvolume multisample fluorimeter with rapid temperature control
    C T Wittwer
    Idaho Technology, Idaho Falls, USA
    Biotechniques 22:176-81. 1997
    ..Complete amplification and analysis requires only 10-15 min...
  14. pmc RET proto-oncogene genotyping using unlabeled probes, the masking technique, and amplicon high-resolution melting analysis
    Rebecca L Margraf
    Advanced Technology Group, ARUP Institute for Clinical and Experimental Pathology, 500 Chipeta Way, Salt Lake City, UT 84108, USA
    J Mol Diagn 9:184-96. 2007
    ..Amplicon high-resolution melting analysis with unlabeled probes and the masking technique is a fast, accurate method for genotyping the >50 RET sequence variations...
  15. ncbi request reprint Closed-tube genotyping of apolipoprotein E by isolated-probe PCR with multiple unlabeled probes and high-resolution DNA melting analysis
    Matthew Dean Poulson
    Department of Pathology, University of Utah, Salt Lake City 84132, USA
    Biotechniques 43:87-91. 2007
    ..The IP-PCR technique should also be useful in detecting SNPs in other high-GC regions of the human genome...
  16. ncbi request reprint Solution-phase DNA mutation scanning and SNP genotyping by nanoliter melting analysis
    Scott O Sundberg
    Department of Bioengineering, University of Utah, SLC, UT 84112, USA
    Biomed Microdevices 9:159-66. 2007
    ..Microscale systems for performing DNA melting reduce the reagents/DNA template required with a promise for high throughput analysis in a closed chamber without risk of contamination...
  17. ncbi request reprint Expanded instrument comparison of amplicon DNA melting analysis for mutation scanning and genotyping
    Mark G Herrmann
    Institute for Clinical and Experimental Pathology, ARUP, Salt Lake City, UT 84108, USA
    Clin Chem 53:1544-8. 2007
    ..We assessed the performance of these new instruments for genotyping and scanning for mutations...
  18. pmc Unlabeled oligonucleotides as internal temperature controls for genotyping by amplicon melting
    Michael T Seipp
    ARUP Institute for Clinical and Experimental, Pathology, Salt Lake City, UT 84108, USA
    J Mol Diagn 9:284-9. 2007
    ..In conclusion, internal temperature controls increase the accuracy of genotyping by high-resolution amplicon melting and should also improve results on lower resolution instruments...
  19. doi request reprint Enrichment and detection of rare alleles by means of snapback primers and rapid-cycle PCR
    Luming Zhou
    Department of Pathology, University of Utah Health Sciences Center, Salt Lake City, UT 84132, USA
    Clin Chem 56:814-22. 2010
    ..Selective amplification of minority alleles is often necessary to detect cancer mutations in clinical samples...
  20. pmc LightCycler technology in molecular diagnostics
    Elaine Lyon
    Department of Pathology, University of Utah, Salt Lake City, Utah, USA
    J Mol Diagn 11:93-101. 2009
    ..High-resolution melting allows mutation scanning by detecting all heterozygous changes. This review describes the major advances throughout the last 15 years regarding LightCycler technology and its application in clinical laboratories...
  21. pmc Rapid genetic analysis of x-linked chronic granulomatous disease by high-resolution melting
    Harry R Hill
    Department of Pathology, Pediatrics and Medicine, 5B114, University of Utah School of Medicine, 50 N Medical Dr, Salt Lake City, UT 84132, USA
    J Mol Diagn 12:368-76. 2010
    ..High-resolution melting can provide timely diagnosis at low cost for effective clinical management of rare, genetic primary immunodeficiency disorders...
  22. doi request reprint Family clusters of variant X-linked chronic granulomatous disease
    Jeffrey M Bender
    Department of Pediatrics, University of Utah School of Medicine, Salt Lake City, Utah, USA
    Pediatr Infect Dis J 28:529-33. 2009
    ..A high index of suspicion in the setting of unusual infections such as Burkholderia cepacia pneumonia is essential to make the diagnosis. Family screening can lead to early intervention, prophylaxis, and appropriate genetic counseling...
  23. doi request reprint Spinning disk platform for microfluidic digital polymerase chain reaction
    Scott O Sundberg
    University of Utah, Rm 5R441, 1795 E South Campus Dr, Salt Lake City, Utah 84112, USA
    Anal Chem 82:1546-50. 2010
    ..The spinning disk platform reduces disposable cost, instrument complexity, and thermocycling time compared to other current digital PCR platforms...
  24. pmc High resolution melting applications for clinical laboratory medicine
    Maria Erali
    ARUP Institute for Clinical and Experimental Pathology, University of Utah, Salt Lake City, UT 84108, USA
    Exp Mol Pathol 85:50-8. 2008
    ..In the clinical laboratory, it is an ideal format for in-house testing, with minimal cost and time requirements for new assay development...
  25. ncbi request reprint Quantitative heteroduplex analysis for single nucleotide polymorphism genotyping
    Robert A Palais
    Department of Mathematics, University of Utah, Salt Lake City, UT 84112, USA
    Anal Biochem 346:167-75. 2005
    ..Optimal mixing before PCR followed by high-resolution melting analysis permits genotyping of all SNPs with a single closed-tube analysis...
  26. ncbi request reprint Real-time PCR technology for cancer diagnostics
    Philip S Bernard
    Department of Pathology, University of Utah School of Medicine, 30 North 1900 East, Salt Lake City 84132, USA
    Clin Chem 48:1178-85. 2002
    ..The challenge for pathology will be the development and implementation of these molecular classifications for routine clinical practice...
  27. ncbi request reprint Distinguishing different DNA heterozygotes by high-resolution melting
    Robert Graham
    Department of Pathology, University of Utah School of Medicine, Salt Lake City 84132, USA
    Clin Chem 51:1295-8. 2005
  28. ncbi request reprint Quantification of low-copy transcripts by continuous SYBR Green I monitoring during amplification
    T B Morrison
    Department of Pathology, University of Utah Medical School, Salt Lake City 84132, USA
    Biotechniques 24:954-8, 960, 962. 1998
    ..Above 10 copies per reaction, typical replicate coefficients of variation were 6%-37%, with better precision at higher copy numbers...
  29. ncbi request reprint Quantification of HER2/neu gene amplification by competitive pcr using fluorescent melting curve analysis
    E Lyon
    Department of Pathology, School of Medicine, University of Utah, Salt Lake 84132, USA
    Clin Chem 47:844-51. 2001
    ..We designed a quantitative PCR system utilizing fluorescent hybridization probes and a competitor that differed from the HER2/neu sequence by a single base change...
  30. ncbi request reprint A comparison of high-resolution melting analysis with denaturing high-performance liquid chromatography for mutation scanning: cystic fibrosis transmembrane conductance regulator gene as a model
    Lan Szu Chou
    Institute for Clinical and Experimental Pathology, ARUP Laboratories, Salt Lake City, UT 84108 1221, USA
    Am J Clin Pathol 124:330-8. 2005
    ..HRMA had better sensitivity and specificity than dHPLC with the added advantage that some homozygous sequence alterations could be identified. HRMA has great potential for rapid, closed-tube mutation scanning...
  31. ncbi request reprint Detection of c-kit-activating mutations in gastrointestinal stromal tumors by high-resolution amplicon melting analysis
    Carlynn Willmore
    Department of Pathology, University of Utah, Salt Lake City 84108, USA
    Am J Clin Pathol 122:206-16. 2004
    ..High-resolution melting analysis can be used to scan DNA for potential c-kit-activating mutations and can aid in the diagnosis of GISTs...
  32. ncbi request reprint Unlabeled probes for the detection and typing of herpes simplex virus
    Shale Dames
    ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, UT 84108, USA
    Clin Chem 53:1847-54. 2007
    ..Unlabeled probes and amplicon melting have been used to detect small deletions and single-nucleotide polymorphisms in assays where template is in abundance. Unlabeled probes have not been applied to low-level target detection, however...
  33. pmc Rapid diagnosis of MEN2B using unlabeled probe melting analysis and the LightCycler 480 instrument
    Rebecca L Margraf
    ARUP Institute for Clinical and Experimental Pathology, University of Utah Medical School, Salt Lake City, Utah, USA
    J Mol Diagn 10:123-8. 2008
    ..This assay demonstrates 100% specificity and sensitivity for the identification of RET mutations causative of MEN2B...
  34. ncbi request reprint Sensitivity and specificity of single-nucleotide polymorphism scanning by high-resolution melting analysis
    Gudrun H Reed
    Department of Pathology, University of Utah Medical Center, 5B418, 50 N Medical Dr, Salt Lake City 84132, USA
    Clin Chem 50:1748-54. 2004
    ..Screening for heterozygous sequence changes in PCR products, also known as "mutation scanning", is an important tool for genetic research and clinical applications. Conventional methods require a separation step...
  35. pmc The use of EGFR exon 19 and 21 unlabeled DNA probes to screen for activating mutations in non-small cell lung cancer
    Carlynn Willmore-Payne
    Associated Regional and University Pathologists Institute for Clinical and Experimental Patholog y, Salt Lake City, UT 84108, USA
    J Biomol Tech 19:217-24. 2008
    ..This allows for easy identification of activating mutations even in homozygous or amplified states and is useful in the screening of NSCLC for the common EGFR activating mutations...
  36. ncbi request reprint Closed-tube genotyping with unlabeled oligonucleotide probes and a saturating DNA dye
    Luming Zhou
    Department of Pathology, University of Utah School of Medicine, Salt Lake City, UT 84132, USA
    Clin Chem 50:1328-35. 2004
    ..However, some single-nucleotide polymorphisms (SNPs) could not be completely genotyped without addition of a known genotype, and high-resolution melting techniques were necessary...
  37. pmc Genotyping hepatitis C virus by heteroduplex mobility analysis using temperature gradient capillary electrophoresis
    Rebecca L Margraf
    ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, Utah 84108, USA
    J Clin Microbiol 42:4545-51. 2004
    ..Compared to conventional HMA, TGCE improves the resolution, with better separation of heteroduplexes and homoduplexes. All common HCV genotypes can be detected and differentiated by this HMA-TGCE assay...
  38. ncbi request reprint Real-time polymerase chain reaction and melting curve analysis
    Robert J Pryor
    Department of Pathology, University of Utah School of Medicine, Salt Lake City, UT, USA
    Methods Mol Biol 336:19-32. 2006
    ..When used together with rapid temperature control, these methods allow amplification and genotyping in less than a half hour...
  39. ncbi request reprint Evaluation of Her-2/neu gene status in osteosarcoma by fluorescence in situ hybridization and multiplex and monoplex polymerase chain reactions
    Carlynn Willmore-Payne
    Department of Pathology, University of Utah Health Sciences Center, Salt Lake City, UT 84132, USA
    Arch Pathol Lab Med 130:691-8. 2006
    ..Previous reports suggest that the human epidermal growth factor 2 (HER-2/neu) receptor may be overexpressed in osteosarcoma...
  40. ncbi request reprint Simultaneous mutation scanning and genotyping by high-resolution DNA melting analysis
    Jesse Montgomery
    Department of Pathology, UUMC, 5B418, 50 N Medical Drive, Salt Lake City, Utah 84105, USA
    Nat Protoc 2:59-66. 2007
    ..The PCR requires <30 min (capillaries) or 1.5 h (96- or 384-well plates) and melting acquisition takes 1-2 min per capillary or 5 min per plate...
  41. doi request reprint A rapid and efficient method of genotyping zebrafish mutants
    John M Parant
    Department of Neurobiology and Anatomy, University of Utah School of Medicine, Salt Lake City, Utah 84112, USA
    Dev Dyn 238:3168-74. 2009
    ..This technique can genotype individual zebrafish embryos and adults (by tail-clip) and is applicable to other model organisms...
  42. doi request reprint Plastic versus glass capillaries for rapid-cycle PCR
    Oluwole Elenitoba-Johnson
    University of Utah, Salt Lake City, Utah 84132, USA
    Biotechniques 44:487-8, 490, 492. 2008
    ..Only the APOE target was successfully amplified by extending the denaturation and annealing times to 5 or 10 s. A 20 s holding period was necessary to reach target temperatures in plastic capillaries...
  43. ncbi request reprint Masking selected sequence variation by incorporating mismatches into melting analysis probes
    Rebecca L Margraf
    ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, Utah 84108, USA
    Hum Mutat 27:269-78. 2006
    ..The masking probes can also localize mutations to specific codons or nucleotide positions. Masking probes can simplify melting analysis of complex regions and eliminate the need for sequencing...
  44. pmc Rapid molecular analysis of the STAT3 gene in Job syndrome of hyper-IgE and recurrent infectious diseases
    Attila Kumanovics
    Department of Pathology, Human Genetics, University of Utah School of Medicine and the Associated Regional and University Pathologists Institute for Clinical and Experimental Pathology, Salt Lake City, UT, USA
    J Mol Diagn 12:213-9. 2010
    ....
  45. pmc Human papillomavirus genotyping using an automated film-based chip array
    Maria Erali
    ARUP Laboratories, 500 Chipeta Way, Salt Lake City, UT 84108, USA
    J Mol Diagn 11:439-45. 2009
    ..Based on its analytical performance, future studies with this platform are warranted to assess its clinical utility for HPV detection and genotyping...
  46. ncbi request reprint Identifying common genetic variants by high-resolution melting
    Joshua G Vandersteen
    Department of Pathology, University of Utah Health Sciences Center, Salt Lake City, UT 84132, USA
    Clin Chem 53:1191-8. 2007
    ..Heteroduplex scanning techniques usually detect all heterozygotes, including common variants not of clinical interest...
  47. ncbi request reprint Homogeneous amplification and mutation scanning of the p53 gene using fluorescent melting curves
    Haleigh Millward
    Idaho Technology Inc, Salt Lake City, UT 84105, USA
    Clin Chem 48:1321-8. 2002
    ..In malignancy, gene mutations frequently occur in tumor suppressor genes such as p53 and are sporadically located. We describe a homogeneous method for amplification and mutation scanning, and apply the method to the p53 gene...
  48. doi request reprint Snapback primer genotyping with saturating DNA dye and melting analysis
    Luming Zhou
    Department of Pathology, University of Utah Health Sciences Center, Salt Lake City, Utah 84132, USA
    Clin Chem 54:1648-56. 2008
    ..DNA hairpins have been used in molecular analysis of PCR products as self-probing amplicons. Either physical separation or fluorescent oligonucleotides with covalent modifications were previously necessary...
  49. ncbi request reprint High-resolution DNA melting curve analysis to establish HLA genotypic identity
    L Zhou
    Department of Pathology, University of Utah, Salt Lake City, 84132, USA
    Tissue Antigens 64:156-64. 2004
    ..The technique may also prove useful for confirmation of HLA genotypic identity between unrelated individuals prior to allogeneic hematopoietic stem-cell transplantation...
  50. ncbi request reprint High-resolution DNA melting analysis for simple and efficient molecular diagnostics
    Gudrun H Reed
    Department of Pathology, University of Utah Medical Center, 5B418, 50 North Medical Drive, Salt Lake City, UT 84132, USA
    Pharmacogenomics 8:597-608. 2007
    ..Owing to its simplicity and speed, the method is a good fit for personalized medicine as a rapid, inexpensive method to predict therapeutic response...
  51. doi request reprint High-resolution DNA melting analysis in clinical research and diagnostics
    Jesse L Montgomery
    Department of Pathology, University of Utah Health Sciences Center, Salt Lake City, UT 84132, USA
    Expert Rev Mol Diagn 10:219-40. 2010
    ....
  52. ncbi request reprint Mutation scanning of the RET protooncogene using high-resolution melting analysis
    Rebecca L Margraf
    Advanced Technology Group, ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, UT 84108, USA
    Clin Chem 52:138-41. 2006
    ..The current widely used approach for RET mutation detection is sequencing of the exons...
  53. pmc Comparison of two quantitative polymerase chain reaction methods for detecting HER2/neu amplification
    Alison Millson
    Associated Regional and University Pathologists Institute for Clinical and Experimental Pathology, Salt Lake City, Utah, USA
    J Mol Diagn 5:184-90. 2003
    ..Real-time quantification and microdissection is useful clinically for HER2/neu quantification. Its ease of use and broad dynamic range allows screening for amplification of HER2/neu...
  54. doi request reprint Mathematical algorithms for high-resolution DNA melting analysis
    Robert Palais
    Department of Mathematics, University of Utah, Salt Lake City, Utah, USA
    Methods Enzymol 454:323-43. 2009
    ....
  55. pmc Multiplex amplicon genotyping by high-resolution melting
    Michael T Seipp
    ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, Utah 84108, USA
    J Biomol Tech 20:160-4. 2009
    ..The LS-32 simplifies more complex high-resolution melting assays by reducing hands-on manipulation, total time of analysis, and reagent cost while maintaining the resolution necessary for multiplex amplicon genotyping...
  56. ncbi request reprint Molecular classification of melanoma using real-time quantitative reverse transcriptase-polymerase chain reaction
    Tracey B Lewis
    Research and Development, ARUP Laboratories Inc, Salt Lake City, Utah, USA
    Cancer 104:1678-86. 2005
    ..Molecular methods are more sensitive in detecting occult lymph node metastases compared with standard histopathology and are reported to have utility in clinical diagnostics...
  57. ncbi request reprint Quadruplex genotyping of F5, F2, and MTHFR variants in a single closed tube by high-resolution amplicon melting
    Michael T Seipp
    ARUP Institute for Clinical and Experimental Pathology, ARUP Laboratories, 500 Chipeta Way, Salt Lake City, UT 84108, USA
    Clin Chem 54:108-15. 2008
    ....
  58. ncbi request reprint Real-time PCR quantification of AT1 and AT2 angiotensin receptor mRNA expression in the developing rat kidney
    Pilar Garcia-Villalba
    Department of Pediatrics, University of Utah School of Medicine, Salt Lake City, Utah 84132 2204, USA
    Nephron Exp Nephrol 94:e154-9. 2003
    ..AT1 promotes growth and proliferation and mediates many of the known physiological actions of Ang II. AT2 appears to antagonize AT1. Our goal was to measure their relative contributions to Ang II signaling in the developing kidney...
  59. ncbi request reprint Single-tube method for nucleic acid extraction, amplification, purification, and sequencing
    Rebecca L Margraf
    ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, UT 84108, USA
    Clin Chem 50:1755-61. 2004
    ..We used a commercially available nucleic acid extraction system to develop a single-tube extraction-to-sequencing (STETS) method for HCV genotyping...
  60. ncbi request reprint Rapid species identification within the Mycobacterium chelonae-abscessus group by high-resolution melting analysis of hsp65 PCR products
    Ian D Odell
    Department of Pathology, University of Utah Medical School, Salt Lake City, 84132, USA
    Am J Clin Pathol 123:96-101. 2005
    ..Advantages of the method include speed, low risk of amplicon contamination (closed-tube), and no need for separation steps (sequencing, electrophoresis, high-performance liquid chromatography) or real-time monitoring...
  61. doi request reprint Multiple endocrine neoplasia type 2 RET protooncogene database: repository of MEN2-associated RET sequence variation and reference for genotype/phenotype correlations
    Rebecca L Margraf
    ARUP Institute for Clinical and Experimental Pathology R, Salt Lake City, Utah 84108, USA
    Hum Mutat 30:548-56. 2009
    ..The MEN2 RET database (www.arup.utah.edu/database/MEN2/MEN2_welcome.php) will serve as a repository for MEN2-associated RET sequence variation and reference for RET genotype/MEN2 phenotype correlations...
  62. ncbi request reprint Instrument comparison for heterozygote scanning of single and double heterozygotes: a correction and extension of Herrmann et al., Clin Chem 2006;52:494-503
    Mark G Herrmann
    Clin Chem 53:150-2. 2007
  63. ncbi request reprint Multiplex single-color PCR with amplicon melting analysis for identification of Aspergillus species
    Maria Erali
    Clin Chem 52:1443-5. 2006
  64. ncbi request reprint Fifty years of molecular (DNA/RNA) diagnostics
    Thomas R Gingeras
    Affymetrix, Inc, Santa Clara, CA, USA
    Clin Chem 51:661-71. 2005

Research Grants5

  1. HOMOGENEOUS MULTIPLEX PCR BY FLUORESCENCE AND TM
    Carl Wittwer; Fiscal Year: 2002
    ..This will be the first real-time instrument compatible with all visible/near IR fluorescent probes and amplification techniques. Multiplexing by fluorescence and Tm greatly expands the power of real-time nucleic acid analysis. ..
  2. Homogeneous Mutation Scanning
    Carl Wittwer; Fiscal Year: 2006
    ....
  3. A System for Rapid PCR, Mutation Scanning and Genotyping
    Carl Wittwer; Fiscal Year: 2006
    ....