Research Topics
Genomes and Genes | George WitmanSummaryAffiliation: University of Massachusetts Medical School Country: USA Publications
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Publications
Cell motility: deaf Drosophila keep the beatGeorge B Witman
Department of Cell Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
Curr Biol 13:R796-8. 2003..Two recent studies have shown that defects in intraflagellar transport prevent assembly of sensory cilia in Drosophila, leaving the fly deaf and uncoordinated. Surprisingly, the mutant sperm flagella have normal structure and motility...
IC97 is a novel intermediate chain of I1 dynein that interacts with tubulin and regulates interdoublet slidingMaureen Wirschell
Department of Cell Biology, Emory University School of Medicine, Atlanta, GA 30322, USA
Mol Biol Cell 20:3044-54. 2009....- CEP290 tethers flagellar transition zone microtubules to the membrane and regulates flagellar protein contentBranch Craige
Department of Cell Biology, University of Massachusetts Medical School, Worcester, MA 01655, USA
J Cell Biol 190:927-40. 2010..The results indicate that CEP290 is required to form microtubule-membrane linkers that tether the flagellar membrane to the transition zone microtubules, and is essential for controlling flagellar protein composition... - Chlamydomonas reinhardtii hydin is a central pair protein required for flagellar motilityKarl Ferdinand Lechtreck
Department of Cell Biology, University of Massachusetts Medical School, Worcester, MA 01655, USA
J Cell Biol 176:473-82. 2007..Hydrocephalus caused by mutations in hydin likely involves the malfunctioning of cilia because of a defect in the CP... - Functional analysis of an individual IFT protein: IFT46 is required for transport of outer dynein arms into flagellaYuqing Hou
Department of Cell Biology, University of Massachusetts Medical School, Worcester, MA 01655, USA
J Cell Biol 176:653-65. 2007..Axonemal ultrastructure is restored, except that the outer arms are still missing, although outer arm subunits are present in the cytoplasm. Thus, IFT46 is specifically required for transporting outer arms into the flagellum... - DC3, the 21-kDa subunit of the outer dynein arm-docking complex (ODA-DC), is a novel EF-hand protein important for assembly of both the outer arm and the ODA-DCDiane M Casey
Department of Cell Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
Mol Biol Cell 14:3650-63. 2003..The results indicate that DC3 is important for both outer arm and ODA-DC assembly... - Mutations in Hydin impair ciliary motility in miceKarl Ferdinand Lechtreck
Department of Cell, University of Massachusetts Medical School, Worcester, MA 01655, USA
J Cell Biol 180:633-43. 2008..Similar defects are observed for cilia in trachea. We conclude that hydrocephalus in hydin mutants is caused by a central pair defect impairing ciliary motility and fluid transport in the brain... - The vertebrate primary cilium is a sensory organelleGregory J Pazour
Program in Molecular Medicine, University of Massachusetts Medical School, 373 Plantation Street, Worcester, MA 01605, USA
Curr Opin Cell Biol 15:105-10. 2003..Primary cilia on other cell types specifically display different receptors, including those for somatostatin and serotonin... - HA-tagging of putative flagellar proteins in Chlamydomonas reinhardtii identifies a novel protein of intraflagellar transport complex BKarl Ferdinand Lechtreck
Department of Cell Biology, University of Massachusetts Medical School, Worcester, Massachusetts, USA
Cell Motil Cytoskeleton 66:469-82. 2009..Cell Motil. Cytoskeleton 2009. (c) 2009 Wiley-Liss, Inc... - The Chlamydomonas reinhardtii BBSome is an IFT cargo required for export of specific signaling proteins from flagellaKarl Ferdinand Lechtreck
Department of Cell Biology, University of Massachusetts Medical School, Worcester, 01655, USA
J Cell Biol 187:1117-32. 2009..We conclude that the BBSome is carried by IFT but is an adapter rather than an integral component of the IFT machinery. C. reinhardtii BBS4 may be required for the export of signaling proteins from the flagellum via IFT... - Proteomic analysis of a eukaryotic ciliumGregory J Pazour
Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA
J Cell Biol 170:103-13. 2005..The flagellum also contains many proteins that are conserved in humans but have not been previously characterized in any organism. The results indicate that flagella are far more complex than previously estimated... - The outer dynein arm-docking complex: composition and characterization of a subunit (oda1) necessary for outer arm assemblySaeko Takada
Department of Cell Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
Mol Biol Cell 13:1015-29. 2002..These results demonstrate that the ODA1 gene encodes the M(r) approximately 70,000 protein, and that the protein is essential for assembly of the ODA-DC and the outer dynein arm onto the doublet microtubule... - The intraflagellar transport protein, IFT88, is essential for vertebrate photoreceptor assembly and maintenanceGregory J Pazour
Department of Cell Biology, University of Massachusetts Medical School, Worcester, MA 01655, USA
J Cell Biol 157:103-13. 2002..We further find that mice with a mutation in the IFT particle protein gene, Tg737/IFT88, have abnormal OS development and retinal degeneration. Thus, IFT is important for assembly and maintenance of the vertebrate OS... - Oda5p, a novel axonemal protein required for assembly of the outer dynein arm and an associated adenylate kinaseMaureen Wirschell
Department of Cell Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
Mol Biol Cell 15:2729-41. 2004..We propose that Oda5p is part of a novel axonemal complex that is required for outer arm assembly and anchors adenylate kinase in proximity to the arm... - Novel role for a sterol response element binding protein in directing spermatogenic cell-specific gene expressionHang Wang
Department of Physiology, University of Massachusetts Medical School, 55 Lake Avenue N, Worcester, MA 01655 0127 USA
Mol Cell Biol 24:10681-8. 2004..We propose that SREBP2gc is part of a cadre of spermatogenic cell-enriched isoforms of ubiquitously expressed transcriptional coregulators that were specifically adapted in concert to direct differentiation of the male germ cell lineage... - A dynein light intermediate chain, D1bLIC, is required for retrograde intraflagellar transportYuqing Hou
Department of Cell Biology, University of Massachusetts Medical School, Worcester, MA 01655, USA
Mol Biol Cell 15:4382-94. 2004..The constructs rescued the mutant cells to a wild-type phenotype, indicating that the function of D1bLIC in IFT is independent of its P-loop... - DC3, the smallest subunit of the Chlamydomonas flagellar outer dynein arm-docking complex, is a redox-sensitive calcium-binding proteinDiane M Casey
Department of Cell Biology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA
J Biol Chem 278:42652-9. 2003..Thus, DC3 is a true calcium-binding protein, but the function of this activity remains unknown... - Pericentrin forms a complex with intraflagellar transport proteins and polycystin-2 and is required for primary cilia assemblyAgata Jurczyk
Department of Molecular Medicine, University of Massachusetts Medical School, 373 Plantation Street, Biotech II, Suite 210, Worcester, MA 01605, USA
J Cell Biol 166:637-43. 2004..We conclude that Pcnt, IFTs, and PC2 form a complex in vertebrate cells that is required for assembly of primary cilia and possibly motile cilia and flagella... 
High-speed digital imaging of ependymal cilia in the murine brainKarl Ferdinand Lechtreck
Department of Cell Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
Methods Cell Biol 91:255-64. 2009..The technique is useful for detailed assessment of how various conditions or mutations affect the fidelity of ciliary motility at the ependyma. The methods are also applicable to other ciliated epithelia, for example, in airways...- Radial spoke proteins of Chlamydomonas flagellaPinfen Yang
Department of Biological Sciences, Marquette University, Milwaukee, WI 53233, USA
J Cell Sci 119:1165-74. 2006..The human genes encoding these proteins are candidates for causing primary ciliary dyskinesia, a severe inherited disease involving missing or defective axonemal structures, including the radial spokes... - Polycystin-2 localizes to kidney cilia and the ciliary level is elevated in orpk mice with polycystic kidney diseaseGregory J Pazour
Curr Biol 12:R378-80. 2002 - The autosomal recessive polycystic kidney disease protein is localized to primary cilia, with concentration in the basal body areaShixuan Wang
Renal Division, Department of Medicine, Brigham and Women s Hospital and Harvard Medical School, Boston, MA 02115, USA
J Am Soc Nephrol 15:592-602. 2004..It is proposed that the pathogenesis of autosomal recessive polycystic kidney disease is linked to the dysfunction of primary cilia... - Function and dynamics of PKD2 in Chlamydomonas reinhardtii flagellaKaiyao Huang
Department of Molecular Cell and Developmental Biology, Yale University, New Haven, CT 06520, USA
J Cell Biol 179:501-14. 2007..These results suggest that the CrPKD2 cation channel is involved in coupling flagellar adhesion at the beginning of mating to the increase in flagellar calcium required for subsequent steps in mating... - Intraflagellar transportJoel L Rosenbaum
Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut 06520, USA
Nat Rev Mol Cell Biol 3:813-25. 2002..Recent results indicate that defects in IFT might be a primary cause of some human diseases... - Differential light chain assembly influences outer arm dynein motor functionLinda M DiBella
Department of Molecular, Microbial, and Structural Biology, University of Connecticut Health Center, Farmington, CT 06030-3305, USA
Mol Biol Cell 16:5661-74. 2005..These data suggest that LC2, LC6, and LC9 have different roles in outer arm assembly and are required for wild-type motor function in the Chlamydomonas flagellum... - The Chlamydomonas genome reveals the evolution of key animal and plant functionsSabeeha S Merchant
Department of Chemistry and Biochemistry, University of California at Los Angeles, Los Angeles, CA 90095, USA
Science 318:245-50. 2007....
Research Grants
- Flagellar Motility and AssemlbyGeorge Witman; Fiscal Year: 2007..The research will provide new information on the role of cilia and flagella in cystic kidney disease, retinal degeneration, primary ciliary dyskinesia, male infertility, and hydrocephalus. ..
- CELL AND MOLECULAR BIOLOGY OF REPRODUCTIONGeorge Witman; Fiscal Year: 2007..The program will help meet the national need for highly skilled young investigators who can bring modern cell and molecular biological approaches to bear on important problems in reproductive biology. ..
- Flagellar Motility and AssemblyGeorge Witman; Fiscal Year: 2006..The studies promise to increase our understanding of a wide range of human diseases, including primary ciliary dyskinesia, in which the dynein arms are frequently missing, male infertility, and thyroid disorders. ..
- MOLECULAR MECHANISM OF FLAGELLAR MOTILITYGeorge Witman; Fiscal Year: 2002..abstract_text> ..