George Witman

Summary

Affiliation: University of Massachusetts Medical School
Country: USA

Publications

  1. pmc CEP290 tethers flagellar transition zone microtubules to the membrane and regulates flagellar protein content
    Branch Craige
    Department of Cell Biology, University of Massachusetts Medical School, Worcester, MA 01655, USA
    J Cell Biol 190:927-40. 2010
  2. pmc Functional analysis of an individual IFT protein: IFT46 is required for transport of outer dynein arms into flagella
    Yuqing Hou
    Department of Cell Biology, University of Massachusetts Medical School, Worcester, MA 01655, USA
    J Cell Biol 176:653-65. 2007
  3. pmc Proteomic analysis of a eukaryotic cilium
    Gregory J Pazour
    Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA
    J Cell Biol 170:103-13. 2005
  4. ncbi request reprint Cell motility: deaf Drosophila keep the beat
    George B Witman
    Department of Cell Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    Curr Biol 13:R796-8. 2003
  5. pmc IC97 is a novel intermediate chain of I1 dynein that interacts with tubulin and regulates interdoublet sliding
    Maureen Wirschell
    Department of Cell Biology, Emory University School of Medicine, Atlanta, GA 30322, USA
    Mol Biol Cell 20:3044-54. 2009
  6. pmc Mutations in Hydin impair ciliary motility in mice
    Karl Ferdinand Lechtreck
    Department of Cell, University of Massachusetts Medical School, Worcester, MA 01655, USA
    J Cell Biol 180:633-43. 2008
  7. ncbi request reprint The vertebrate primary cilium is a sensory organelle
    Gregory J Pazour
    Program in Molecular Medicine, University of Massachusetts Medical School, 373 Plantation Street, Worcester, MA 01605, USA
    Curr Opin Cell Biol 15:105-10. 2003
  8. pmc HA-tagging of putative flagellar proteins in Chlamydomonas reinhardtii identifies a novel protein of intraflagellar transport complex B
    Karl Ferdinand Lechtreck
    Department of Cell Biology, University of Massachusetts Medical School, Worcester, Massachusetts, USA
    Cell Motil Cytoskeleton 66:469-82. 2009
  9. pmc DC3, the 21-kDa subunit of the outer dynein arm-docking complex (ODA-DC), is a novel EF-hand protein important for assembly of both the outer arm and the ODA-DC
    Diane M Casey
    Department of Cell Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    Mol Biol Cell 14:3650-63. 2003
  10. pmc Chlamydomonas reinhardtii hydin is a central pair protein required for flagellar motility
    Karl Ferdinand Lechtreck
    Department of Cell Biology, University of Massachusetts Medical School, Worcester, MA 01655, USA
    J Cell Biol 176:473-82. 2007

Research Grants

  1. Flagellar Motility and Assemlby
    George Witman; Fiscal Year: 2007
  2. CELL AND MOLECULAR BIOLOGY OF REPRODUCTION
    George Witman; Fiscal Year: 2007
  3. Flagellar Motility and Assembly
    George Witman; Fiscal Year: 2006
  4. Flagellar Motility and Assembly
    George Witman; Fiscal Year: 2005
  5. Flagellar Motility and Assembly
    George Witman; Fiscal Year: 2004
  6. Flagellar Motility and Assembly
    George Witman; Fiscal Year: 2003
  7. MOLECULAR MECHANISM OF FLAGELLAR MOTILITY
    George Witman; Fiscal Year: 2002
  8. MOLECULAR MECHANISM OF FLAGELLAR MOTILITY
    George Witman; Fiscal Year: 2001
  9. MOLECULAR MECHANISM OF FLAGELLAR MOTILITY
    George Witman; Fiscal Year: 2000
  10. MOLECULAR MECHANISM OF FLAGELLAR MOTILITY
    George Witman; Fiscal Year: 1999

Collaborators

Detail Information

Publications26

  1. pmc CEP290 tethers flagellar transition zone microtubules to the membrane and regulates flagellar protein content
    Branch Craige
    Department of Cell Biology, University of Massachusetts Medical School, Worcester, MA 01655, USA
    J Cell Biol 190:927-40. 2010
    ..The results indicate that CEP290 is required to form microtubule-membrane linkers that tether the flagellar membrane to the transition zone microtubules, and is essential for controlling flagellar protein composition...
  2. pmc Functional analysis of an individual IFT protein: IFT46 is required for transport of outer dynein arms into flagella
    Yuqing Hou
    Department of Cell Biology, University of Massachusetts Medical School, Worcester, MA 01655, USA
    J Cell Biol 176:653-65. 2007
    ..Axonemal ultrastructure is restored, except that the outer arms are still missing, although outer arm subunits are present in the cytoplasm. Thus, IFT46 is specifically required for transporting outer arms into the flagellum...
  3. pmc Proteomic analysis of a eukaryotic cilium
    Gregory J Pazour
    Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA
    J Cell Biol 170:103-13. 2005
    ..The flagellum also contains many proteins that are conserved in humans but have not been previously characterized in any organism. The results indicate that flagella are far more complex than previously estimated...
  4. ncbi request reprint Cell motility: deaf Drosophila keep the beat
    George B Witman
    Department of Cell Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    Curr Biol 13:R796-8. 2003
    ..Two recent studies have shown that defects in intraflagellar transport prevent assembly of sensory cilia in Drosophila, leaving the fly deaf and uncoordinated. Surprisingly, the mutant sperm flagella have normal structure and motility...
  5. pmc IC97 is a novel intermediate chain of I1 dynein that interacts with tubulin and regulates interdoublet sliding
    Maureen Wirschell
    Department of Cell Biology, Emory University School of Medicine, Atlanta, GA 30322, USA
    Mol Biol Cell 20:3044-54. 2009
    ....
  6. pmc Mutations in Hydin impair ciliary motility in mice
    Karl Ferdinand Lechtreck
    Department of Cell, University of Massachusetts Medical School, Worcester, MA 01655, USA
    J Cell Biol 180:633-43. 2008
    ..Similar defects are observed for cilia in trachea. We conclude that hydrocephalus in hydin mutants is caused by a central pair defect impairing ciliary motility and fluid transport in the brain...
  7. ncbi request reprint The vertebrate primary cilium is a sensory organelle
    Gregory J Pazour
    Program in Molecular Medicine, University of Massachusetts Medical School, 373 Plantation Street, Worcester, MA 01605, USA
    Curr Opin Cell Biol 15:105-10. 2003
    ..Primary cilia on other cell types specifically display different receptors, including those for somatostatin and serotonin...
  8. pmc HA-tagging of putative flagellar proteins in Chlamydomonas reinhardtii identifies a novel protein of intraflagellar transport complex B
    Karl Ferdinand Lechtreck
    Department of Cell Biology, University of Massachusetts Medical School, Worcester, Massachusetts, USA
    Cell Motil Cytoskeleton 66:469-82. 2009
    ..Cell Motil. Cytoskeleton 2009. (c) 2009 Wiley-Liss, Inc...
  9. pmc DC3, the 21-kDa subunit of the outer dynein arm-docking complex (ODA-DC), is a novel EF-hand protein important for assembly of both the outer arm and the ODA-DC
    Diane M Casey
    Department of Cell Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    Mol Biol Cell 14:3650-63. 2003
    ..The results indicate that DC3 is important for both outer arm and ODA-DC assembly...
  10. pmc Chlamydomonas reinhardtii hydin is a central pair protein required for flagellar motility
    Karl Ferdinand Lechtreck
    Department of Cell Biology, University of Massachusetts Medical School, Worcester, MA 01655, USA
    J Cell Biol 176:473-82. 2007
    ..Hydrocephalus caused by mutations in hydin likely involves the malfunctioning of cilia because of a defect in the CP...
  11. pmc The Chlamydomonas reinhardtii BBSome is an IFT cargo required for export of specific signaling proteins from flagella
    Karl Ferdinand Lechtreck
    Department of Cell Biology, University of Massachusetts Medical School, Worcester, 01655, USA
    J Cell Biol 187:1117-32. 2009
    ..We conclude that the BBSome is carried by IFT but is an adapter rather than an integral component of the IFT machinery. C. reinhardtii BBS4 may be required for the export of signaling proteins from the flagellum via IFT...
  12. pmc The intraflagellar transport protein, IFT88, is essential for vertebrate photoreceptor assembly and maintenance
    Gregory J Pazour
    Department of Cell Biology, University of Massachusetts Medical School, Worcester, MA 01655, USA
    J Cell Biol 157:103-13. 2002
    ..We further find that mice with a mutation in the IFT particle protein gene, Tg737/IFT88, have abnormal OS development and retinal degeneration. Thus, IFT is important for assembly and maintenance of the vertebrate OS...
  13. pmc The outer dynein arm-docking complex: composition and characterization of a subunit (oda1) necessary for outer arm assembly
    Saeko Takada
    Department of Cell Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    Mol Biol Cell 13:1015-29. 2002
    ..These results demonstrate that the ODA1 gene encodes the M(r) approximately 70,000 protein, and that the protein is essential for assembly of the ODA-DC and the outer dynein arm onto the doublet microtubule...
  14. pmc Oda5p, a novel axonemal protein required for assembly of the outer dynein arm and an associated adenylate kinase
    Maureen Wirschell
    Department of Cell Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    Mol Biol Cell 15:2729-41. 2004
    ..We propose that Oda5p is part of a novel axonemal complex that is required for outer arm assembly and anchors adenylate kinase in proximity to the arm...
  15. pmc Pericentrin forms a complex with intraflagellar transport proteins and polycystin-2 and is required for primary cilia assembly
    Agata Jurczyk
    Department of Molecular Medicine, University of Massachusetts Medical School, 373 Plantation Street, Biotech II, Suite 210, Worcester, MA 01605, USA
    J Cell Biol 166:637-43. 2004
    ..We conclude that Pcnt, IFTs, and PC2 form a complex in vertebrate cells that is required for assembly of primary cilia and possibly motile cilia and flagella...
  16. pmc A dynein light intermediate chain, D1bLIC, is required for retrograde intraflagellar transport
    Yuqing Hou
    Department of Cell Biology, University of Massachusetts Medical School, Worcester, MA 01655, USA
    Mol Biol Cell 15:4382-94. 2004
    ..The constructs rescued the mutant cells to a wild-type phenotype, indicating that the function of D1bLIC in IFT is independent of its P-loop...
  17. ncbi request reprint DC3, the smallest subunit of the Chlamydomonas flagellar outer dynein arm-docking complex, is a redox-sensitive calcium-binding protein
    Diane M Casey
    Department of Cell Biology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA
    J Biol Chem 278:42652-9. 2003
    ..Thus, DC3 is a true calcium-binding protein, but the function of this activity remains unknown...
  18. pmc Novel role for a sterol response element binding protein in directing spermatogenic cell-specific gene expression
    Hang Wang
    Department of Physiology, University of Massachusetts Medical School, 55 Lake Avenue N, Worcester, MA 01655 0127 USA
    Mol Cell Biol 24:10681-8. 2004
    ..We propose that SREBP2gc is part of a cadre of spermatogenic cell-enriched isoforms of ubiquitously expressed transcriptional coregulators that were specifically adapted in concert to direct differentiation of the male germ cell lineage...
  19. pmc High-speed digital imaging of ependymal cilia in the murine brain
    Karl Ferdinand Lechtreck
    Department of Cell Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    Methods Cell Biol 91:255-64. 2009
    ..The technique is useful for detailed assessment of how various conditions or mutations affect the fidelity of ciliary motility at the ependyma. The methods are also applicable to other ciliated epithelia, for example, in airways...
  20. ncbi request reprint Polycystin-2 localizes to kidney cilia and the ciliary level is elevated in orpk mice with polycystic kidney disease
    Gregory J Pazour
    Curr Biol 12:R378-80. 2002
  21. pmc Radial spoke proteins of Chlamydomonas flagella
    Pinfen Yang
    Department of Biological Sciences, Marquette University, Milwaukee, WI 53233, USA
    J Cell Sci 119:1165-74. 2006
    ..The human genes encoding these proteins are candidates for causing primary ciliary dyskinesia, a severe inherited disease involving missing or defective axonemal structures, including the radial spokes...
  22. pmc Function and dynamics of PKD2 in Chlamydomonas reinhardtii flagella
    Kaiyao Huang
    Department of Molecular Cell and Developmental Biology, Yale University, New Haven, CT 06520, USA
    J Cell Biol 179:501-14. 2007
    ..These results suggest that the CrPKD2 cation channel is involved in coupling flagellar adhesion at the beginning of mating to the increase in flagellar calcium required for subsequent steps in mating...
  23. pmc The Chlamydomonas genome reveals the evolution of key animal and plant functions
    Sabeeha S Merchant
    Department of Chemistry and Biochemistry, University of California at Los Angeles, Los Angeles, CA 90095, USA
    Science 318:245-50. 2007
    ....
  24. pmc Differential light chain assembly influences outer arm dynein motor function
    Linda M DiBella
    Department of Molecular, Microbial, and Structural Biology, University of Connecticut Health Center, Farmington, CT 06030 3305, USA
    Mol Biol Cell 16:5661-74. 2005
    ..These data suggest that LC2, LC6, and LC9 have different roles in outer arm assembly and are required for wild-type motor function in the Chlamydomonas flagellum...
  25. ncbi request reprint The autosomal recessive polycystic kidney disease protein is localized to primary cilia, with concentration in the basal body area
    Shixuan Wang
    Renal Division, Department of Medicine, Brigham and Women s Hospital and Harvard Medical School, Boston, MA 02115, USA
    J Am Soc Nephrol 15:592-602. 2004
    ..It is proposed that the pathogenesis of autosomal recessive polycystic kidney disease is linked to the dysfunction of primary cilia...
  26. ncbi request reprint Intraflagellar transport
    Joel L Rosenbaum
    Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut 06520, USA
    Nat Rev Mol Cell Biol 3:813-25. 2002
    ..Recent results indicate that defects in IFT might be a primary cause of some human diseases...

Research Grants14

  1. Flagellar Motility and Assemlby
    George Witman; Fiscal Year: 2007
    ..The research will provide new information on the role of cilia and flagella in cystic kidney disease, retinal degeneration, primary ciliary dyskinesia, male infertility, and hydrocephalus. ..
  2. CELL AND MOLECULAR BIOLOGY OF REPRODUCTION
    George Witman; Fiscal Year: 2007
    ..The program will help meet the national need for highly skilled young investigators who can bring modern cell and molecular biological approaches to bear on important problems in reproductive biology. ..
  3. Flagellar Motility and Assembly
    George Witman; Fiscal Year: 2006
    ..The studies promise to increase our understanding of a wide range of human diseases, including primary ciliary dyskinesia, in which the dynein arms are frequently missing, male infertility, and thyroid disorders. ..
  4. Flagellar Motility and Assembly
    George Witman; Fiscal Year: 2005
    ..The studies promise to increase our understanding of a wide range of human diseases, including primary ciliary dyskinesia, in which the dynein arms are frequently missing, male infertility, and thyroid disorders. ..
  5. Flagellar Motility and Assembly
    George Witman; Fiscal Year: 2004
    ..The studies promise to increase our understanding of a wide range of human diseases, including primary ciliary dyskinesia, in which the dynein arms are frequently missing, male infertility, and thyroid disorders. ..
  6. Flagellar Motility and Assembly
    George Witman; Fiscal Year: 2003
    ..The studies promise to increase our understanding of a wide range of human diseases, including primary ciliary dyskinesia, in which the dynein arms are frequently missing, male infertility, and thyroid disorders. ..
  7. MOLECULAR MECHANISM OF FLAGELLAR MOTILITY
    George Witman; Fiscal Year: 2002
    ..abstract_text> ..
  8. MOLECULAR MECHANISM OF FLAGELLAR MOTILITY
    George Witman; Fiscal Year: 2001
    ..abstract_text> ..
  9. MOLECULAR MECHANISM OF FLAGELLAR MOTILITY
    George Witman; Fiscal Year: 2000
    ..abstract_text> ..
  10. MOLECULAR MECHANISM OF FLAGELLAR MOTILITY
    George Witman; Fiscal Year: 1999
    ..abstract_text> ..