Research Topics
Genomes and Genes | Arlene A WiseSummaryAffiliation: University of Pennsylvania Country: USA Publications
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Publications
Intersubunit complementation of sugar signal transduction in VirA heterodimers and posttranslational regulation of VirA activity in Agrobacterium tumefaciensArlene A Wise
Department of Biology, University of Pennsylvania, Philadelphia, PA 19104 6018, USA
J Bacteriol 187:213-23. 2005..Moreover, constitutive expression of virG overcame the inhibitory effect of some but not all virA alleles, suggesting that there may be more than one inhibitory mechanism...
The receiver domain of hybrid histidine kinase VirA: an enhancing factor for vir gene expression in Agrobacterium tumefaciensArlene A Wise
Department of Biology, University of Pennsylvania, Philadelphia, PA 19104 6018, USA
J Bacteriol 192:1534-42. 2010..These phenotypes were corrected if the virG copy number was increased. In addition, we show that the VirA receiver domain can interact with the VirG DNA-binding domain...
Nucleic acid extraction from Agrobacterium strainsArlene A Wise
Plant Science Institute, Department of Biology, University of Pennsylvania, PA, USA
Methods Mol Biol 343:67-76. 2006..In addition, we provide a simple method for the electronic transfer of shuttle plasmids from Agrobacterium to E. coli for use when their low copy number in Agrobacterium impedes plasmid isolation from that strain...
Three methods for the introduction of foreign DNA into AgrobacteriumArlene A Wise
Plant Science Institute, Department of Biology, University of Pennsylvania, PA, USA
Methods Mol Biol 343:43-53. 2006..Here we present three detailed protocols for Agrobacterium strain construction using electroporation, the freeze/thaw method of transformation, and triparental mating...
The integrity of the periplasmic domain of the VirA sensor kinase is critical for optimal coordination of the virulence signal response in Agrobacterium tumefaciensGauri R Nair
Department of Biology, University of Pennsylvania, 415 South University Ave, Philadelphia, PA 19104 6018, USA
J Bacteriol 193:1436-48. 2011..These experiments provide evidence that small perturbations in the periplasmic domain of VirA can uncouple sugar-mediated changes in AS sensitivity from the sugar-mediated effects on maximal activity...
The plant signal salicylic acid shuts down expression of the vir regulon and activates quormone-quenching genes in AgrobacteriumZe Chun Yuan
Department of Microbiology, University of Washington, Seattle, WA 98195, USA
Proc Natl Acad Sci U S A 104:11790-5. 2007..Our results illustrate that SA, besides its well known function in regulating plant defense, can also interfere directly with several aspects of the Agrobacterium infection process...
