THOMAS EDWARD WILSON

Summary

Affiliation: University of Michigan
Country: USA

Publications

  1. pmc Saccharomyces cerevisiae DNA ligase IV supports imprecise end joining independently of its catalytic activity
    Kishore K Chiruvella
    Department of Pathology, University of Michigan, Ann Arbor, MI, USA
    PLoS Genet 9:e1003599. 2013
  2. pmc De novo CNV formation in mouse embryonic stem cells occurs in the absence of Xrcc4-dependent nonhomologous end joining
    Martin F Arlt
    Department of Human Genetics, University of Michigan, Ann Arbor, Michigan, United States of America
    PLoS Genet 8:e1002981. 2012
  3. doi request reprint Building on the past, shaping the future: the Environmental Mutagenesis and Genomics Society
    Thomas E Wilson
    Department of Pathology, University of Michigan Medical School, Ann Arbor, Michigan 48109 2200, USA
    Environ Mol Mutagen 54:153-7. 2013
  4. pmc A genomics-based screen for yeast mutants with an altered recombination/end-joining repair ratio
    Thomas E Wilson
    Department of Pathology, University of Michigan Medical School, Ann Arbor 48109 0602, USA
    Genetics 162:677-88. 2002
  5. ncbi request reprint Non-homologous end-joining: bacteria join the chromosome breakdance
    Thomas E Wilson
    Department of Pathology, University of Michigan Medical School, Ann Arbor, MI, 48109 0602, USA
    Trends Biochem Sci 28:62-6. 2003
  6. pmc Mutations of the Yku80 C terminus and Xrs2 FHA domain specifically block yeast nonhomologous end joining
    Phillip L Palmbos
    Department of Pathology, University of Michigan Medical School, Ann Arbor, 48109 0602, USA
    Mol Cell Biol 25:10782-90. 2005
  7. ncbi request reprint Nonhomologous end joining in yeast
    James M Daley
    Cellular and Molecular Biology Program, University of Michigan Medical School, Ann Arbor, Michigan 48109 0602, USA
    Annu Rev Genet 39:431-51. 2005
  8. pmc Abrogation of the Chk1-Pds1 checkpoint leads to tolerance of persistent single-strand breaks in Saccharomyces cerevisiae
    Anandi S Karumbati
    Department of Pathology, University of Michigan Medical School, Ann Arbor, 48109 0602, USA
    Genetics 169:1833-44. 2005
  9. ncbi request reprint The role of yeast DNA 3'-phosphatase Tpp1 and rad1/Rad10 endonuclease in processing spontaneous and induced base lesions
    Anandi S Karumbati
    Department of Pathology, University of Michigan Medical School, Ann Arbor, Michigan 48109 0602, USA
    J Biol Chem 278:31434-43. 2003
  10. pmc Rejoining of DNA double-strand breaks as a function of overhang length
    James M Daley
    Department of Pathology, University of Michigan Medical School, Medical Science I M4214 0602, 1301 Catherine Road, Ann Arbor, MI 48109 0602, USA
    Mol Cell Biol 25:896-906. 2005

Research Grants

  1. End Processing in DNA Double Strand Break Repair
    Thomas Wilson; Fiscal Year: 2001
  2. Genetics of Yeast Nonhomologous End-Joining
    Thomas Wilson; Fiscal Year: 2007
  3. Systematic Genetic Analysis of Yeast NHEJ
    Thomas Wilson; Fiscal Year: 2006
  4. Systematic Genetic Analysis of Yeast NHEJ
    Thomas Wilson; Fiscal Year: 2005
  5. Genetics of Yeast Nonhomologous End-Joining
    Thomas Wilson; Fiscal Year: 2004
  6. End Processing in DNA Double Strand Break Repair
    Thomas Wilson; Fiscal Year: 2004
  7. End Processing in DNA Double Strand Break Repair
    Thomas Wilson; Fiscal Year: 2003
  8. End Processing in DNA Double Strand Break Repair
    Thomas Wilson; Fiscal Year: 2002
  9. Systematic Genetic Analysis of Yeast NHEJ
    THOMAS EDWARD WILSON; Fiscal Year: 2010

Collaborators

Detail Information

Publications18

  1. pmc Saccharomyces cerevisiae DNA ligase IV supports imprecise end joining independently of its catalytic activity
    Kishore K Chiruvella
    Department of Pathology, University of Michigan, Ann Arbor, MI, USA
    PLoS Genet 9:e1003599. 2013
    ..These findings indicate that Dnl4 can promote mutagenic end joining independently of its catalytic activity, likely by a mechanism that involves Cdc9. ..
  2. pmc De novo CNV formation in mouse embryonic stem cells occurs in the absence of Xrcc4-dependent nonhomologous end joining
    Martin F Arlt
    Department of Human Genetics, University of Michigan, Ann Arbor, Michigan, United States of America
    PLoS Genet 8:e1002981. 2012
    ..These results establish that nonrecurrent CNVs can be, and frequently are, formed by mechanisms other than Xrcc4-dependent NHEJ...
  3. doi request reprint Building on the past, shaping the future: the Environmental Mutagenesis and Genomics Society
    Thomas E Wilson
    Department of Pathology, University of Michigan Medical School, Ann Arbor, Michigan 48109 2200, USA
    Environ Mol Mutagen 54:153-7. 2013
    ....
  4. pmc A genomics-based screen for yeast mutants with an altered recombination/end-joining repair ratio
    Thomas E Wilson
    Department of Pathology, University of Michigan Medical School, Ann Arbor 48109 0602, USA
    Genetics 162:677-88. 2002
    ..These results are discussed in the context of the minimal set of required proteins and regulatory inputs for NHEJ...
  5. ncbi request reprint Non-homologous end-joining: bacteria join the chromosome breakdance
    Thomas E Wilson
    Department of Pathology, University of Michigan Medical School, Ann Arbor, MI, 48109 0602, USA
    Trends Biochem Sci 28:62-6. 2003
    ....
  6. pmc Mutations of the Yku80 C terminus and Xrs2 FHA domain specifically block yeast nonhomologous end joining
    Phillip L Palmbos
    Department of Pathology, University of Michigan Medical School, Ann Arbor, 48109 0602, USA
    Mol Cell Biol 25:10782-90. 2005
    ..These results identify a novel role in yeast NHEJ for the poorly characterized Ku80 C-terminal and Xrs2 FHA domains, and they suggest that redundant binding of DNA ligase IV facilitates completion of this DNA repair event...
  7. ncbi request reprint Nonhomologous end joining in yeast
    James M Daley
    Cellular and Molecular Biology Program, University of Michigan Medical School, Ann Arbor, Michigan 48109 0602, USA
    Annu Rev Genet 39:431-51. 2005
    ..We also consider how the balance between NHEJ and homologous repair is regulated by cell state to promote genome preservation. The principles discussed are instructive to NHEJ in all organisms...
  8. pmc Abrogation of the Chk1-Pds1 checkpoint leads to tolerance of persistent single-strand breaks in Saccharomyces cerevisiae
    Anandi S Karumbati
    Department of Pathology, University of Michigan Medical School, Ann Arbor, 48109 0602, USA
    Genetics 169:1833-44. 2005
    ..We propose a model in which recombinational repair during S phase coupled with failure of the metaphase-anaphase checkpoint allows for tolerance of persistent single-strand breaks at the expense of genome stability...
  9. ncbi request reprint The role of yeast DNA 3'-phosphatase Tpp1 and rad1/Rad10 endonuclease in processing spontaneous and induced base lesions
    Anandi S Karumbati
    Department of Pathology, University of Michigan Medical School, Ann Arbor, Michigan 48109 0602, USA
    J Biol Chem 278:31434-43. 2003
    ....
  10. pmc Rejoining of DNA double-strand breaks as a function of overhang length
    James M Daley
    Department of Pathology, University of Michigan Medical School, Medical Science I M4214 0602, 1301 Catherine Road, Ann Arbor, MI 48109 0602, USA
    Mol Cell Biol 25:896-906. 2005
    ..These data support a model in which annealing energy is a primary determinant of the rejoining efficiency and mechanism...
  11. ncbi request reprint Identification of DNA 3'-phosphatase active site residues and their differential role in DNA binding, Mg2+ coordination, and catalysis
    Rajashree A Deshpande
    Department of Pathology, University of Michigan Medical School, 1301 Catherine Road, M4214 Medical Sciences Building I, Box 0602, Ann Arbor, Michigan 48109 0602, USA
    Biochemistry 43:8579-89. 2004
    ..On the basis of these similarities in mutant phenotypes of Tpp1 and phosphoserine phosphatase, we propose a reaction mechanism for Tpp1 which explains its strict phosphate specificity...
  12. pmc Recruitment of Saccharomyces cerevisiae Dnl4-Lif1 complex to a double-strand break requires interactions with Yku80 and the Xrs2 FHA domain
    Phillip L Palmbos
    Department of Pathology, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA
    Genetics 180:1809-19. 2008
    ..The collected results indicate that the Xrs-Lif1 and Yku80-Dnl4 interactions are important for formation of a productive ligase-DSB intermediate...
  13. pmc Recruitment and dissociation of nonhomologous end joining proteins at a DNA double-strand break in Saccharomyces cerevisiae
    Dongliang Wu
    Department of Pathology, University of Michigan Medical School, Ann Arbor, Michigan 48109 2200, USA
    Genetics 178:1237-49. 2008
    ..These findings reveal the in vivo assembly of the NHEJ repair complex and shed light on the mechanisms controlling DSB repair pathway utilization...
  14. pmc Evidence that base stacking potential in annealed 3' overhangs determines polymerase utilization in yeast nonhomologous end joining
    James M Daley
    Graduate Program in Cellular and Molecular Biology and Department of Pathology, University of Michigan Medical School, 2065 BSRB, 109 Zina Pitcher Place, Ann Arbor, MI 48109 2200, United States
    DNA Repair (Amst) 7:67-76. 2008
    ..The 5' flap endonuclease Rad27 was not required for repair in this or any situation tested, indicating that still other NHEJ 5' nucleases must exist...
  15. pmc Modes of interaction among yeast Nej1, Lif1 and Dnl4 proteins and comparison to human XLF, XRCC4 and Lig4
    Rajashree A Deshpande
    Department of Pathology, University of Michigan Medical School, Room 2065 BSRB, 109 Zina Pitcher Place, Ann Arbor, MI 48109 2200, USA
    DNA Repair (Amst) 6:1507-16. 2007
    ..The implications of these findings for DNA ligase IV function are considered in light of the evolutionary pattern in the XLF/XRCC4 and XLF/Nej1 family...
  16. ncbi request reprint DNA joint dependence of pol X family polymerase action in nonhomologous end joining
    James M Daley
    Graduate Program in Cellular and Molecular Biology and Department of Pathology, University of Michigan Medical School, Ann Arbor, MI 48109 0602, USA
    J Biol Chem 280:29030-7. 2005
    ..Finally, mammalian Pol X polymerases were able to differentially complement a pol4 mutation depending on the joint structure, demonstrating that these polymerases can participate in yeast NHEJ but with distinct properties...
  17. pmc Enhancement of Saccharomyces cerevisiae end-joining efficiency by cell growth stage but not by impairment of recombination
    Elissa Karathanasis
    Department of Pathology, University of Michigan Medical School, 1301 Catherine Road, M4214 Med Sci I, Ann Arbor, MI 48109 0602, USA
    Genetics 161:1015-27. 2002
    ..The combined results argue against a model in which pathway utilization is determined by a passive competition. Instead, they demonstrate an active regulation designed to optimize the likelihood of genome restoration based on cell state...
  18. pmc Increased recombination between active tRNA genes
    Matthew J Pratt-Hyatt
    Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor, 48109 0606, USA
    DNA Cell Biol 25:359-64. 2006
    ....

Research Grants10

  1. End Processing in DNA Double Strand Break Repair
    Thomas Wilson; Fiscal Year: 2001
    ..This assay, in vitro biochemical assays, and cellular responses to chemical mutagens will evaluate the hypothesis that ORF YMR156c is the 3' phosphatase portion of yeast polynucleotide kinase. ..
  2. Genetics of Yeast Nonhomologous End-Joining
    Thomas Wilson; Fiscal Year: 2007
    ..Specific Aim #4 seeks to identify NHEJ core complex mutants that are specifically deficient in joining incompatible DNA ends, hypothesized to be deficient in recruiting polymerases and nucleases. ..
  3. Systematic Genetic Analysis of Yeast NHEJ
    Thomas Wilson; Fiscal Year: 2006
    ..Specific Aim #4 seeks to identify NHEJ core complex mutants that are specifically deficient in joining incompatible DNA ends, hypothesized to be deficient in recruiting polymerases and nucleases. ..
  4. Systematic Genetic Analysis of Yeast NHEJ
    Thomas Wilson; Fiscal Year: 2005
    ..Specific Aim #4 seeks to identify NHEJ core complex mutants that are specifically deficient in joining incompatible DNA ends, hypothesized to be deficient in recruiting polymerases and nucleases. ..
  5. Genetics of Yeast Nonhomologous End-Joining
    Thomas Wilson; Fiscal Year: 2004
    ..Specific Aim #4 seeks to identify NHEJ core complex mutants that are specifically deficient in joining incompatible DNA ends, hypothesized to be deficient in recruiting polymerases and nucleases. ..
  6. End Processing in DNA Double Strand Break Repair
    Thomas Wilson; Fiscal Year: 2004
    ..This assay, in vitro biochemical assays, and cellular responses to chemical mutagens will evaluate the hypothesis that ORF YMR156c is the 3' phosphatase portion of yeast polynucleotide kinase. ..
  7. End Processing in DNA Double Strand Break Repair
    Thomas Wilson; Fiscal Year: 2003
    ..This assay, in vitro biochemical assays, and cellular responses to chemical mutagens will evaluate the hypothesis that ORF YMR156c is the 3' phosphatase portion of yeast polynucleotide kinase. ..
  8. End Processing in DNA Double Strand Break Repair
    Thomas Wilson; Fiscal Year: 2002
    ..This assay, in vitro biochemical assays, and cellular responses to chemical mutagens will evaluate the hypothesis that ORF YMR156c is the 3' phosphatase portion of yeast polynucleotide kinase. ..
  9. Systematic Genetic Analysis of Yeast NHEJ
    THOMAS EDWARD WILSON; Fiscal Year: 2010
    ....