David Wilson

Summary

Affiliation: University of Pennsylvania
Country: USA

Publications

  1. doi request reprint Measuring oxygen in living tissue: intravascular, interstitial, and "tissue" oxygen measurements
    David F Wilson
    Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, PA 19104, USA
    Adv Exp Med Biol 701:53-9. 2011
  2. doi request reprint Oxygen, pH, and mitochondrial oxidative phosphorylation
    David F Wilson
    Department of Biochemistry and Biophysics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
    J Appl Physiol 113:1838-45. 2012
  3. ncbi request reprint Monitoring the dynamics of tissue oxygenation in vivo by phosphorescence quenching
    David F Wilson
    Department of Biochemistry and Biophysics, School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
    Adv Exp Med Biol 540:1-5. 2003
  4. pmc Immediate and long-term responses of the carotid body to high altitude
    David F Wilson
    Department of Biochemistry and Biophysics, University of Pennsylvania Medical Center, Philadelphia, PA 19104, USA
    High Alt Med Biol 6:97-111. 2005
  5. ncbi request reprint Oxygen pressures in the interstitial space and their relationship to those in the blood plasma in resting skeletal muscle
    David F Wilson
    Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, PA 19104, USA
    J Appl Physiol 101:1648-56. 2006
  6. ncbi request reprint Quantifying the role of oxygen pressure in tissue function
    David F Wilson
    Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, PA 19104, USA
    Am J Physiol Heart Circ Physiol 294:H11-3. 2008
  7. pmc Oxygen distribution and vascular injury in the mouse eye measured by phosphorescence-lifetime imaging
    David F Wilson
    Department of Biochemistry, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Appl Opt 44:5239-48. 2005
  8. ncbi request reprint Measurement of tumor oxygenation using new frequency domain phosphorometers
    David F Wilson
    Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, PA 19104 6059, USA
    Comp Biochem Physiol A Mol Integr Physiol 132:153-9. 2002
  9. pmc Oxygen-dependent quenching of phosphorescence used to characterize improved myocardial oxygenation resulting from vasculogenic cytokine therapy
    William Hiesinger
    Division of Cardiovascular Surgery, Department of Surgery, University of Pennsylvania, Philadelphia, PA 19104, USA
    J Appl Physiol 110:1460-5. 2011
  10. ncbi request reprint A method for measuring oxygen distributions in tissue using frequency domain phosphorometry
    Sergei A Vinogradov
    Department of Biochemistry and Biophysics, School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
    Comp Biochem Physiol A Mol Integr Physiol 132:147-52. 2002

Collaborators

Detail Information

Publications41

  1. doi request reprint Measuring oxygen in living tissue: intravascular, interstitial, and "tissue" oxygen measurements
    David F Wilson
    Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, PA 19104, USA
    Adv Exp Med Biol 701:53-9. 2011
    ....
  2. doi request reprint Oxygen, pH, and mitochondrial oxidative phosphorylation
    David F Wilson
    Department of Biochemistry and Biophysics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
    J Appl Physiol 113:1838-45. 2012
    ....
  3. ncbi request reprint Monitoring the dynamics of tissue oxygenation in vivo by phosphorescence quenching
    David F Wilson
    Department of Biochemistry and Biophysics, School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
    Adv Exp Med Biol 540:1-5. 2003
  4. pmc Immediate and long-term responses of the carotid body to high altitude
    David F Wilson
    Department of Biochemistry and Biophysics, University of Pennsylvania Medical Center, Philadelphia, PA 19104, USA
    High Alt Med Biol 6:97-111. 2005
    ..In the chronically adapted carotid body, responses to acute changes in oxygen pressure are enhanced. The adaptive changes due to chronic hypoxia are largely reversed upon return to lower altitudes...
  5. ncbi request reprint Oxygen pressures in the interstitial space and their relationship to those in the blood plasma in resting skeletal muscle
    David F Wilson
    Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, PA 19104, USA
    J Appl Physiol 101:1648-56. 2006
    ..It was, therefore, not further used...
  6. ncbi request reprint Quantifying the role of oxygen pressure in tissue function
    David F Wilson
    Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, PA 19104, USA
    Am J Physiol Heart Circ Physiol 294:H11-3. 2008
  7. pmc Oxygen distribution and vascular injury in the mouse eye measured by phosphorescence-lifetime imaging
    David F Wilson
    Department of Biochemistry, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Appl Opt 44:5239-48. 2005
    ..High-resolution maps of oxygen distribution in the retina can provide greater understanding of the role of oxygen and vascular function in diseases of the eye...
  8. ncbi request reprint Measurement of tumor oxygenation using new frequency domain phosphorometers
    David F Wilson
    Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, PA 19104 6059, USA
    Comp Biochem Physiol A Mol Integr Physiol 132:153-9. 2002
    ..This procedure determines the tissue oxygen distribution at each position in the grid. Regions of relative hypoxia (associated with the tumor) can be readily localized and the extent of hypoxia quantitatively evaluated...
  9. pmc Oxygen-dependent quenching of phosphorescence used to characterize improved myocardial oxygenation resulting from vasculogenic cytokine therapy
    William Hiesinger
    Division of Cardiovascular Surgery, Department of Surgery, University of Pennsylvania, Philadelphia, PA 19104, USA
    J Appl Physiol 110:1460-5. 2011
    ..This effect could potentially mitigate the vicious cycle of myocyte death, myocardial fibrosis, progressive ventricular dilatation, and eventual heart failure seen after acute myocardial infarction...
  10. ncbi request reprint A method for measuring oxygen distributions in tissue using frequency domain phosphorometry
    Sergei A Vinogradov
    Department of Biochemistry and Biophysics, School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
    Comp Biochem Physiol A Mol Integr Physiol 132:147-52. 2002
    ..Here we describe a multi-frequency system for measurements of oxygen distributions in tissue in vivo...
  11. ncbi request reprint Cholinergic regulation of fuel-induced hormone secretion and respiration of SUR1-/- mouse islets
    Nicolai M Doliba
    Dept of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Am J Physiol Endocrinol Metab 291:E525-35. 2006
    ....
  12. pmc Palmitic acid acutely inhibits acetylcholine- but not GLP-1-stimulated insulin secretion in mouse pancreatic islets
    Nicolai M Doliba
    Department of Biochemistry and Biophysics and Institute for Diabetes, Obesity, and Metabolism, University of Pennsylvania School of Medicine, Philadelphia, 19104 6140, USA
    Am J Physiol Endocrinol Metab 299:E475-85. 2010
    ..We speculate that acute emptying of the ER calcium by PA results in decreased glucose stimulation of respiration and acetylcholine potentiation of insulin secretion...
  13. ncbi request reprint Tissue oxygen tension during regional low-flow perfusion in neonates
    William M DeCampli
    Divisions of Pediatric Cardiothoracic Surgery and Anesthesia and Critical Care Medicine, The Children s Hospital of Philadelphia, PA 19104, USA
    J Thorac Cardiovasc Surg 125:472-80. 2003
    ..We examined cerebral cortical and peripheral organ tissue Po(2) values in a neonatal piglet model of regional low-flow perfusion...
  14. pmc Tomographic imaging of oxygen by phosphorescence lifetime
    Sovia V Apreleva
    Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, PA 19104, USA
    Appl Opt 45:8547-59. 2006
    ..We provide what we believe to be the first example of oxygen imaging in three dimensions using long-lived phosphorescent probes and establish the potential of these probes for diffuse optical tomography...
  15. ncbi request reprint Oxygen distributions in tissue measured by phosphorescence quenching
    Sergei A Vinogradov
    Dept of Biochem and Biophys, Univer of Pennsylvania, Philadelphia, PA 19104, USA
    Adv Exp Med Biol 510:181-5. 2003
  16. pmc Oxygen pressures in the interstitial space of skeletal muscle and tumors in vivo
    David F Wilson
    Department of Biochemistry and Biophysics, Medical School, University of Pennsylvania, Philadelphia, PA 19104, USA
    Adv Exp Med Biol 614:53-62. 2008
    ..In mice, anesthesia with ketamine plus xylazine markedly decreased oxygen pressures in the interstitial and intravascular spaces compared to awake or isoflurane anesthetized mice...
  17. ncbi request reprint Rat carotid body chemosensory discharge and glomus cell HIF-1 alpha expression in vitro: regulation by a common oxygen sensor
    Arijit Roy
    Department of Physiology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104 6059, USA
    Am J Physiol Regul Integr Comp Physiol 293:R829-36. 2007
    ..Although the changes in HIF-1 alpha take minutes and those for CB neural activity occur in 1-2 s, the similar responses to CO and light suggest that the oxygen sensor is the same (mitochondrial cytochrome a(3))...
  18. ncbi request reprint Oxygen distribution in murine tumors: characterization using oxygen-dependent quenching of phosphorescence
    Lisa S Ziemer
    Department of Clinical Studies, Philadelphia School of Veterinary Medicine, Univ of Pennsylvania, Rm 330, BRB II III, 421 Curie Blvd, Philadelphia, PA 19104, USA
    J Appl Physiol 98:1503-10. 2005
    ..The results show that phosphorescence quenching is a reliable, reproducible, and noninvasive method capable of providing real-time determination of oxygen concentrations within tumors...
  19. pmc Feasibility of diffuse optical imaging with long-lived luminescent probes
    Sofia V Apreleva
    Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Opt Lett 31:1082-4. 2006
    ..The approach developed will guide experimental imaging of phosphorescence lifetime and oxygen pressure in living tissue...
  20. ncbi request reprint Effect of hypoxia and ischemia on expression of selected genes in brain of newborn piglets
    Tatiana Zaitseva
    Dept Biochemistry and Biophysics, University of Pennsylvania, School of Medicine, Philadelphia, PA 19104, USA
    Adv Exp Med Biol 510:319-24. 2003
  21. ncbi request reprint Identifying oxygen sensors by their photochemical action spectra
    David F Wilson
    Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia 19104 6059, USA
    Methods Enzymol 381:690-704. 2004
  22. ncbi request reprint Effect of perfusion flow rate on tissue oxygenation in newborn piglets during cardiopulmonary bypass
    Gregory Schears
    Department of Biochemistry and Biophysics, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Ann Thorac Surg 75:560-5. 2003
    ..The present study examined the effects of perfusion flow rate and patent ductus arteriosus (PDA) during normothermic CPB on oxygenation in several organ tissues of newborn piglets...
  23. ncbi request reprint Oxyphor R2 and G2: phosphors for measuring oxygen by oxygen-dependent quenching of phosphorescence
    Isolde Dunphy
    Department of Biochemistry and Biophysics, School of Medicine, University of Pennsylvania, Philadelphia, PA 19104 6059, USA
    Anal Biochem 310:191-8. 2002
    ..4 to 7.8). In vivo application is demonstrated by using Oxyphor G2 to noninvasively determine the oxygen distribution in a subcutaneous tumor growing in rats...
  24. pmc Imaging oxygen pressure in the rodent retina by phosphorescence lifetime
    David F Wilson
    Department of Biochemistry, Medical School, University of Pennsylvania, Philadelphia 19104, USA
    Adv Exp Med Biol 578:119-24. 2006
  25. pmc Response of brain oxygenation and metabolism to deep hypothermic circulatory arrest in newborn piglets: comparison of pH-stat and alpha-stat strategies
    Scott D Markowitz
    Department of Anesthesiology and Critical Care Medicine, The Children s Hospital of Philadelphia, Philadelphia, Pennsylvania, USA
    Ann Thorac Surg 84:170-6. 2007
    ....
  26. pmc Dendritic phosphorescent probes for oxygen imaging in biological systems
    Artem Y Lebedev
    Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    ACS Appl Mater Interfaces 1:1292-304. 2009
    ..The performance of the probes was demonstrated in applications, including in vivo microscopy of vascular pO(2) in the rat brain...
  27. ncbi request reprint Effect of catecholamines on activity of Na(+), K(+)-ATPase in neonatal piglet brain during posthypoxic reoxygenation
    Tatiana Zaitseva
    Department of Biochemistry and Biophysics, School of Medicine, 264 Anatomy Chemistry Building, University of Pennsylvania, Philadelphia, PA 19104, USA
    Comp Biochem Physiol A Mol Integr Physiol 132:139-45. 2002
    ..It is postulated that the decrease in the level of dopamine in the brain prior to ligation and hemorrhage can be at least partly responsible for the observed decrease in activity of Na(+), K(+)-ATPase in the striatum of newborn piglets...
  28. ncbi request reprint Brain oxygenation during cardiopulmonary bypass and circulatory arrest
    Gregory Schears
    Dept of Biochem and Biophys, University of Pennsylvania, School of Medicine, Philadelphia, PA 19104, USA
    Adv Exp Med Biol 510:325-30. 2003
    ..It is suggested that the decreased oxygenation is due to increased heterogeneity in resistance in the microcirculatory units, resulting in broadened distribution of flow rates and oxygen levels...
  29. ncbi request reprint Phosphorescence lifetime imaging in turbid media: the inverse problem and experimental image reconstruction
    Vadim Y Soloviev
    Department of Biochemistry and Biophysics, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Appl Opt 43:564-74. 2004
    ..e., the Tikhonov-Phillips regularization and the maximum entropy method. Theoretical results are experimentally validated, and the reconstructed images of phosphorescent objects rendering oxygen maps in a layer are presented...
  30. pmc Resuscitation with 100%, compared with 21%, oxygen following brief, repeated periods of apnea can protect vulnerable neonatal brain regions from apoptotic injury
    Alberto Mendoza-Paredes
    Department of Biochemistry and Biophysics, The University of Pennsylvania School of Medicine, Philadelphia, PA, USA
    Resuscitation 76:261-70. 2008
    ..21% oxygen enriched gas on levels of key regulatory proteins contributing to cell death (Bax, Caspase-3) or protecting neurons from hypoxic/ischemic injury (Bcl-2, p-Akt, p-CREB)...
  31. ncbi request reprint Comparison of low-flow cardiopulmonary bypass and circulatory arrest on brain oxygen and metabolism
    Steven Schultz
    Department of Anesthesiology and Critical Care, Children s Hospital of Philadelphia, Philadelphia, Pennsylvania, USA
    Ann Thorac Surg 77:2138-43. 2004
    ....
  32. ncbi request reprint Altered gene expression following cardiopulmonary bypass and circulatory arrest
    Tatiana Zaitseva
    Departments of Biochemistry and Biophysics, University of Pennsylvania, School of Medicine, Philadelphia, PA, USA
    Adv Exp Med Biol 530:391-9. 2003
    ..There was no significant alteration in the level of D1AR following DHCA. The results showed that DHCA caused much larger alterations in gene expression in the critical metabolic signaling pathways tested than did CPB...
  33. ncbi request reprint Cerebral oxygenation during repetitive apnea in newborn piglets
    Gregory Schears
    Adv Exp Med Biol 566:1-7. 2005
    ..This deficit in brain oxygenation can be at least partly responsible for the neurological side effects of repetitive apnea...
  34. ncbi request reprint Imaging oxygen pressure in the retina of the mouse eye
    David F Wilson
    Adv Exp Med Biol 566:159-65. 2005
    ....
  35. ncbi request reprint A micro-light guide system for measuring oxygen by phosphorescence quenching
    Leu Wei Lo
    Division of Medical Engineering Research, National Health Research Institutes, Taipei 114, Taiwan
    Adv Exp Med Biol 540:117-23. 2003
  36. ncbi request reprint Measurement of muscle microvascular oxygen pressures: compartmentalization of phosphorescent probe
    David C Poole
    Department of Kinesiology, Kansas State University, Manhattan, Kansas 66506 5802, USA
    Microcirculation 11:317-26. 2004
    ..To determine whether the phosphorescent probe Oxyphor R2 (a palladium porphyrin dendrimer) becomes extravasated within normotensive skeletal muscle, R2 perfusion and washout studies were performed using a perfused rat hindlimb preparation...
  37. doi request reprint The pervasive presence of fluctuating oxygenation in tumors
    Laura I Cárdenas-Navia
    Department of Biomedical Engineering, Duke University, Durham, North Carolina, USA
    Cancer Res 68:5812-9. 2008
    ..The widespread presence of fluctuations in tumor oxygenation has broad ranging implications for tumor progression, stress response, and signal transduction, which are altered by oxygenation/reoxygenation events...
  38. pmc Brain oxygenation and metabolism during repetitive apnea with resuscitation of 21% and 100% oxygen in newborn piglets
    Gregory Schears
    Mayo Clinic, Rochester, and Department of Biochemistry and Biophysics, The University of Pennsylvania, Philadelphia 19104, USA
    Neurochem Res 30:1453-61. 2005
    ..Repetitive apnea caused progressive increase in fraction of hypoxic brain tissue in newborn. The magnitude of the increase is dependent on whether the animals were resuscitated with room air or 100% oxygen...
  39. ncbi request reprint Brain oxygen and metabolism is dependent on the rate of low-flow cardiopulmonary bypass following circulatory arrest in newborn piglets
    Peter Pastuszko
    Department of Surgery, The University of Oklahoma, Oklahoma City, OK, USA
    Eur J Cardiothorac Surg 31:899-905. 2007
    ....
  40. pmc Brain oxygen and metabolism during circulatory arrest with intermittent brief periods of low-flow cardiopulmonary bypass in newborn piglets
    Steven Schultz
    Department of Pediatrics, University of Miami, Miami, Fla, USA
    J Thorac Cardiovasc Surg 132:839-44. 2006
    ....
  41. pmc Brain oxygenation and metabolism during selective cerebral perfusion in neonates
    Gregory Schears
    Department of Anesthesiology and Critical Care, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA
    Eur J Cardiothorac Surg 29:168-74. 2006
    ..To investigate the possible neuroprotective effects of selective cerebral perfusion (SCP) during deep hypothermic circulatory arrest on brain oxygenation and metabolism in newborn piglets...

Research Grants23

  1. DOPAMINE/GLUTAMATE AND HYPOXIC INJURY IN NEWBORN BRAIN
    David Wilson; Fiscal Year: 2003
    ..The role of dopamine, and of dopamine and glutamate receptors on the number of cells undergoing apoptosis and/or necrotic cell death will also be determined by the TUNEL technique. ..
  2. DOPAMINE/GLUTAMATE AND HYPOXIC INJURY IN NEWBORN BRAIN
    David Wilson; Fiscal Year: 2006
    ..This understanding will be the basis for more effective treatment of patients who have been subjected to a period(s) of insufficient oxygen delivery to the brain. ..
  3. DOPAMINE/GLUTAMATE AND HYPOXIC INJURY IN NEWBORN BRAIN
    David Wilson; Fiscal Year: 2007
    ..This understanding will be the basis for more effective treatment of patients who have been subjected to a period(s) of insufficient oxygen delivery to the brain. ..
  4. Nanosensor probes for oxygen imaging in vivo
    David Wilson; Fiscal Year: 2007
    ..e. ready for larger scale synthesis and rigorous toxicology studies preparatory to use in clinical trials. ..
  5. DOPAMINE/GLUTAMATE AND HYPOXIC INJURY IN NEWBORN BRAIN
    David Wilson; Fiscal Year: 2009
    ..This understanding will be the basis for more effective treatment of patients who have been subjected to a period(s) of insufficient oxygen delivery to the brain. ..
  6. OXYGEN LEVELS AND RADIOSENSITIVITY IN TUMORS
    David Wilson; Fiscal Year: 2001
    ..Phosphors will be selected which can be injected intravenously and then, after the oxygen measurements, are excreted by the kidney. ..
  7. DOPAMINE AND HYPOXIC/ISCHEMIC INSULT IN NEWBORN BRAIN
    David Wilson; Fiscal Year: 1999
    ....
  8. VIDEO IMAGING AND NEONATAL BRAIN OXYGENATION
    David Wilson; Fiscal Year: 1993
    ..These measures will permit critical evaluation of the efficacy of treatments designed to optimize oxygen delivery and to reduce irreversible damage to the brain of the newborn...
  9. Nanosensor probes for oxygen imaging in vivo
    David Wilson; Fiscal Year: 2009
    ..e. ready for larger scale synthesis and rigorous toxicology studies preparatory to use in clinical trials. ..