Robert Wightman

Summary

Affiliation: University of North Carolina
Country: USA

Publications

  1. pmc In vivo voltammetry monitoring of electrically evoked extracellular norepinephrine in subregions of the bed nucleus of the stria terminalis
    Natalie R Herr
    Department of Chemistry and Neuroscience Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 3290, USA
    J Neurophysiol 107:1731-7. 2012
  2. pmc PAP and NT5E inhibit nociceptive neurotransmission by rapidly hydrolyzing nucleotides to adenosine
    Sarah E Street
    Department of Cell and Molecular Physiology, University of North Carolina, CB 7545, Chapel Hill, North Carolina 27599, USA
    Mol Pain 7:80. 2011
  3. ncbi request reprint Dopamine release is heterogeneous within microenvironments of the rat nucleus accumbens
    R Mark Wightman
    Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 3290, USA
    Eur J Neurosci 26:2046-54. 2007
  4. ncbi request reprint Detection technologies. Probing cellular chemistry in biological systems with microelectrodes
    R Mark Wightman
    Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Science 311:1570-4. 2006
  5. ncbi request reprint The association of vesicular contents and its effects on release
    R Mark Wightman
    Department of Chemistry and Neuroscience Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 3290, USA
    Ann N Y Acad Sci 971:620-6. 2002
  6. pmc Distinguishing splanchnic nerve and chromaffin cell stimulation in mouse adrenal slices with fast-scan cyclic voltammetry
    Paul L Walsh
    Department of Chemistry, University of North Carolina, Chapel Hill, 27599 3290, USA
    Am J Physiol Cell Physiol 300:C49-57. 2011
  7. pmc Neural encoding of cocaine-seeking behavior is coincident with phasic dopamine release in the accumbens core and shell
    Catarina A Owesson-White
    Department of Psychology, The University of North Carolina, Chapel Hill, NC 27599, USA
    Eur J Neurosci 30:1117-27. 2009
  8. ncbi request reprint Real-time decoding of dopamine concentration changes in the caudate-putamen during tonic and phasic firing
    B Jill Venton
    Department of Chemistry and Neuroscience Center, University of North Carolina, Chapel Hill, NC 27599 3290, USA
    J Neurochem 87:1284-95. 2003
  9. ncbi request reprint Extinction of cocaine self-administration reveals functionally and temporally distinct dopaminergic signals in the nucleus accumbens
    Garret D Stuber
    Curriculum in Neurobiology, The University of North Carolina, Chapel Hill, North Carolina 27599, USA
    Neuron 46:661-9. 2005
  10. ncbi request reprint Real-time measurements of phasic changes in extracellular dopamine concentration in freely moving rats by fast-scan cyclic voltammetry
    Paul E M Phillips
    Department of Chemistry, University of North Carolina at Chapel Hill, NC, USA
    Methods Mol Med 79:443-64. 2003

Research Grants

  1. DYNAMICS OF IN VIVO DOPAMINE RELEASE
    Robert Wightman; Fiscal Year: 2009
  2. DYNAMICS OF IN VIVO DOPAMINE RELEASE
    Robert Wightman; Fiscal Year: 2006
  3. ELECTROANALYSIS OF NEUROTRANSMITTERS AND MODULATORS
    Robert Wightman; Fiscal Year: 2005
  4. ELECTROANALYSIS OF NEUROTRANSMITTERS AND MODULATORS
    Robert Wightman; Fiscal Year: 2007
  5. SECRETION FROM INDIVIDUAL VESICLES
    Robert Wightman; Fiscal Year: 2007
  6. DYNAMICS OF IN VIVO DOPAMINE RELEASE
    Robert Wightman; Fiscal Year: 2007
  7. ELECTROANALYSIS OF NEUROTRANSMITTERS AND MODULATORS
    ROBERT MARK WIGHTMAN; Fiscal Year: 2010
  8. DYNAMICS OF IN VIVO DOPAMINE RELEASE
    ROBERT MARK WIGHTMAN; Fiscal Year: 2010
  9. SECRETION FROM INDIVIDUAL VESICLES
    ROBERT MARK WIGHTMAN; Fiscal Year: 2010
  10. DYNAMICS OF IN VIVO DOPAMINE RELEASE
    Robert Wightman; Fiscal Year: 2009

Collaborators

Detail Information

Publications65

  1. pmc In vivo voltammetry monitoring of electrically evoked extracellular norepinephrine in subregions of the bed nucleus of the stria terminalis
    Natalie R Herr
    Department of Chemistry and Neuroscience Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 3290, USA
    J Neurophysiol 107:1731-7. 2012
    ..Since both local application by iontophoresis and systemic application of IDA had similar effects on NE release, our results indicate that terminal autoreceptors play a predominant role in the inhibition of subsequent release...
  2. pmc PAP and NT5E inhibit nociceptive neurotransmission by rapidly hydrolyzing nucleotides to adenosine
    Sarah E Street
    Department of Cell and Molecular Physiology, University of North Carolina, CB 7545, Chapel Hill, North Carolina 27599, USA
    Mol Pain 7:80. 2011
    ....
  3. ncbi request reprint Dopamine release is heterogeneous within microenvironments of the rat nucleus accumbens
    R Mark Wightman
    Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 3290, USA
    Eur J Neurosci 26:2046-54. 2007
    ..These findings reveal an unanticipated spatial and temporal heterogeneity of dopamine transmission within the NAc that may depend upon the firing of specific subpopulations of dopamine neurons...
  4. ncbi request reprint Detection technologies. Probing cellular chemistry in biological systems with microelectrodes
    R Mark Wightman
    Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Science 311:1570-4. 2006
    ..Such findings can promote interdisciplinary collaborations and may lead to a broader understanding of the chemical aspects of biology...
  5. ncbi request reprint The association of vesicular contents and its effects on release
    R Mark Wightman
    Department of Chemistry and Neuroscience Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 3290, USA
    Ann N Y Acad Sci 971:620-6. 2002
    ..Furthermore, liberation of the Ca(2+) normally stored within the vesicles can promote exocytosis without an external Ca(2+) source...
  6. pmc Distinguishing splanchnic nerve and chromaffin cell stimulation in mouse adrenal slices with fast-scan cyclic voltammetry
    Paul L Walsh
    Department of Chemistry, University of North Carolina, Chapel Hill, 27599 3290, USA
    Am J Physiol Cell Physiol 300:C49-57. 2011
    ..From these results, it was determined that high-intensity stimulations of the adrenal medulla result in depolarizing not only the splanchnic nerves, but also the chromaffin cells themselves resulting in a biphasic catecholamine release...
  7. pmc Neural encoding of cocaine-seeking behavior is coincident with phasic dopamine release in the accumbens core and shell
    Catarina A Owesson-White
    Department of Psychology, The University of North Carolina, Chapel Hill, NC 27599, USA
    Eur J Neurosci 30:1117-27. 2009
    ....
  8. ncbi request reprint Real-time decoding of dopamine concentration changes in the caudate-putamen during tonic and phasic firing
    B Jill Venton
    Department of Chemistry and Neuroscience Center, University of North Carolina, Chapel Hill, NC 27599 3290, USA
    J Neurochem 87:1284-95. 2003
    ..These simulations show that synchronous phasic firing can produce spatially and temporally heterogeneous concentration profiles whereas asynchronous tonic firing elicits uniform, steady-state dopamine concentrations...
  9. ncbi request reprint Extinction of cocaine self-administration reveals functionally and temporally distinct dopaminergic signals in the nucleus accumbens
    Garret D Stuber
    Curriculum in Neurobiology, The University of North Carolina, Chapel Hill, North Carolina 27599, USA
    Neuron 46:661-9. 2005
    ....
  10. ncbi request reprint Real-time measurements of phasic changes in extracellular dopamine concentration in freely moving rats by fast-scan cyclic voltammetry
    Paul E M Phillips
    Department of Chemistry, University of North Carolina at Chapel Hill, NC, USA
    Methods Mol Med 79:443-64. 2003
  11. pmc Preferential enhancement of dopamine transmission within the nucleus accumbens shell by cocaine is attributable to a direct increase in phasic dopamine release events
    Brandon J Aragona
    Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 3290, USA
    J Neurosci 28:8821-31. 2008
    ..Together, these results demonstrate that cocaine administration results in a temporally and regionally specific increase in phasic dopamine release that is significantly regulated by dopamine autoreceptors...
  12. pmc Regional specificity in the real-time development of phasic dopamine transmission patterns during acquisition of a cue-cocaine association in rats
    Brandon J Aragona
    Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 3290, USA
    Eur J Neurosci 30:1889-99. 2009
    ....
  13. pmc Sensitization of rapid dopamine signaling in the nucleus accumbens core and shell after repeated cocaine in rats
    Nii A Addy
    Department of Chemistry, University of North Carolina, Chapel Hill, North Carolina, USA
    J Neurophysiol 104:922-31. 2010
    ..In addition, the data clearly delineate cocaine's release and uptake effects and suggest that the observed sensitization results from greater uptake inhibition in cocaine pre-exposed subjects...
  14. ncbi request reprint Cannabinoids enhance subsecond dopamine release in the nucleus accumbens of awake rats
    Joseph F Cheer
    Department of Chemistry, The University of North Carolina, Chapel Hill, North Carolina 27599 3290, USA
    J Neurosci 24:4393-400. 2004
    ..These data demonstrate that doses of a cannabinoid agonist known to increase burst firing produce ongoing fluctuations in extracellular dopamine on a previously unrecognized temporal scale in the nucleus accumbens...
  15. ncbi request reprint Cocaine increases dopamine release by mobilization of a synapsin-dependent reserve pool
    B Jill Venton
    Department of Chemistry, Neuroscience Center, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    J Neurosci 26:3206-9. 2006
    ..This capacity to enhance exocytotic release of dopamine may be important for the psychostimulant actions of cocaine...
  16. pmc Synapsins differentially control dopamine and serotonin release
    Brian M Kile
    Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    J Neurosci 30:9762-70. 2010
    ..These results provide further support for the concept that synapsin function in presynaptic terminals varies according to the neurotransmitter being released...
  17. ncbi request reprint Rapid dopamine signaling in the nucleus accumbens during contingent and noncontingent cocaine administration
    Garret D Stuber
    Curriculum in Neurobiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Neuropsychopharmacology 30:853-63. 2005
    ....
  18. ncbi request reprint Transient changes in mesolimbic dopamine and their association with 'reward'
    R Mark Wightman
    Department of Chemistry, Neuroscience Center, and Center for Alcohol Studies, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    J Neurochem 82:721-35. 2002
    ..Thus, enhanced dopamine release at terminals appears to be coincident with phasic electrical activity at cell bodies. These accumulating data promise to help unravel the precise role of phasic dopamine transmission in reward processing...
  19. ncbi request reprint Detecting subsecond dopamine release with fast-scan cyclic voltammetry in vivo
    Donita L Robinson
    Department of Chemistry, Neuroscience Center, and Center for Alcohol Studies, University of North Carolina, Chapel Hill, NC 27599 3290, USA
    Clin Chem 49:1763-73. 2003
    ..However, dopamine concentrations are now known to fluctuate on a phasic timescale (subseconds to seconds)...
  20. ncbi request reprint Correlation of local changes in extracellular oxygen and pH that accompany dopaminergic terminal activity in the rat caudate-putamen
    B Jill Venton
    Department of Chemistry and Neuroscience Center, University of North Carolina, Chapel Hill, USA
    J Neurochem 84:373-81. 2003
    ..We conclude that changes in blood flow accompanying terminal activity cause alkaline pH shifts by the rapid removal of carbon dioxide, a component of the extracellular brain buffering system...
  21. ncbi request reprint Coordinated accumbal dopamine release and neural activity drive goal-directed behavior
    Joseph F Cheer
    Department of Chemistry and Neuroscience Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 3290, USA
    Neuron 54:237-44. 2007
    ..This work establishes a temporal link between anticipatory rises of dopamine and firing patterns in the NAc shell during ICSS and suggests that they may play a similar role with natural rewards and during drug self-administration...
  22. ncbi request reprint Dopamine release is severely compromised in the R6/2 mouse model of Huntington's disease
    Michael A Johnson
    Department of Chemistry, The University of North Carolina, Chapel Hill, North Carolina 27599 3290, USA
    J Neurochem 97:737-46. 2006
    ..Collectively, these data demonstrate (i) a reduction in the ability of dopamine terminals to release dopamine and (ii) the importance of this attenuation of release on the motor symptoms of Huntington's disease...
  23. pmc Disparity between tonic and phasic ethanol-induced dopamine increases in the nucleus accumbens of rats
    Donita L Robinson
    Department of Psychiatry, Bowles Center for Alcohol Studies, University of North Carolina DLR, SM, Chapel Hill, North Carolina, USA
    Alcohol Clin Exp Res 33:1187-96. 2009
    ..Acute ethanol increases tonic concentrations of dopamine, but its effect on subsecond dopamine transients has not been fully explored...
  24. ncbi request reprint Associative learning mediates dynamic shifts in dopamine signaling in the nucleus accumbens
    Jeremy J Day
    Department of Psychology, Davie Hall CB no 3270, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Nat Neurosci 10:1020-8. 2007
    ..Consistent with proposed roles in reward prediction and incentive salience, these results indicate that rapid dopamine release provides a reward signal that is dynamically modified by associative learning...
  25. ncbi request reprint Dopamine operates as a subsecond modulator of food seeking
    Mitchell F Roitman
    Department of Psychology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 3270, USA
    J Neurosci 24:1265-71. 2004
    ..Rather, dopamine returned to baseline levels. Together, the results strongly implicate subsecond dopamine signaling in the nucleus accumbens as a real-time modulator of food-seeking behavior...
  26. ncbi request reprint Functional microcircuitry in the accumbens underlying drug addiction: insights from real-time signaling during behavior
    Regina M Carelli
    Department of Psychology, CB 3270, Davie Hall, University of North Carolina, Chapel Hill, North Carolina 27599 3270 USA
    Curr Opin Neurobiol 14:763-8. 2004
    ..These findings are considered with respect to the functional microcircuitry in the nucleus accumbens that underlies goal-directed behavior and the role of this circuit in drug addiction...
  27. ncbi request reprint Temporal separation of vesicle release from vesicle fusion during exocytosis
    Kevin P Troyer
    Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    J Biol Chem 277:29101-7. 2002
    ..These results demonstrate that an osmotic gradient across the fusion pore is an important driving force for exocytotic extrusion of granule contents from secretory cells following fusion pore formation...
  28. pmc Presynaptic dopaminergic function is largely unaltered in mesolimbic and mesostriatal terminals of adult rats that were prenatally exposed to cocaine
    Paul E M Phillips
    Department of Chemistry, Venable Hall CB3290, University of North Carolina, Chapel Hill, NC 27599 3290, USA
    Brain Res 961:63-72. 2003
    ..We conclude, therefore, that behavioral changes that have previously been observed in rats that were prenatally exposed to cocaine are not mediated through alteration of presynaptic dopaminergic mechanisms in these brain regions...
  29. pmc Synaptic overflow of dopamine in the nucleus accumbens arises from neuronal activity in the ventral tegmental area
    Leslie A Sombers
    Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 3290, USA
    J Neurosci 29:1735-42. 2009
    ..The results from these three distinct experiments directly demonstrate, for the first time, how neuronal firing of dopamine neurons originating in the VTA translates into synaptic overflow in a key terminal region, the NAc shell...
  30. pmc In vivo measurement of somatodendritic release of dopamine in the ventral tegmental area
    Justin M Kita
    Department of Chemistry and Neuroscience Center, University of North Carolina, Chapel Hill, North Carolina 27599 3290, USA
    Synapse 63:951-60. 2009
    ..Thus, VTA dopamine release can be evoked in vivo by remote stimulations and it resembles release in terminal regions, possessing a similar uptake mechanism and a finite releasable storage pool...
  31. ncbi request reprint Separating vesicle fusion and exocytosis in hypertonic conditions
    Kevin P Troyer
    Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 3290, USA
    Ann N Y Acad Sci 971:251-3. 2002
    ..Subsequent replacement of the osmotic gradient leads to release of the contents from fused vesicles...
  32. pmc Real-time monitoring of chemical transmission in slices of the murine adrenal gland
    Jelena Petrovic
    Neuroscience Center R M W, Caudill Laboratories, Venable Hall B 5, CB 3290, Chapel Hill, North Carolina 27599 3290, USA
    Endocrinology 151:1773-83. 2010
    ..Whereas adrenergic sites contained mostly epinephrine during electrical stimulation, noradrenergic sites contained a mixture of the catecholamines showing the heterogeneity of the adrenal medulla...
  33. pmc Simultaneous dopamine and single-unit recordings reveal accumbens GABAergic responses: implications for intracranial self-stimulation
    Joseph F Cheer
    Department of Chemistry and Neuroscience Center, University of North Carolina, Chapel Hill, NC 27599, USA
    Proc Natl Acad Sci U S A 102:19150-5. 2005
    ..Thus, neurons in the NAc are preferentially inhibited by GABA(A) receptors after MFB stimulation, a mechanism that may also be important in ICS...
  34. ncbi request reprint Frequency of dopamine concentration transients increases in dorsal and ventral striatum of male rats during introduction of conspecifics
    Donita L Robinson
    Department of Chemistry, Neuroscience Center, and Center for Alcohol Studies, University of North Carolina, Chapel Hill, North Carolina 27599 3290, USA
    J Neurosci 22:10477-86. 2002
    ..These results establish for the first time that transient dopamine fluctuations occur throughout the dorsal and ventral striatum and demonstrate that they are more frequent with salient stimuli that elicit a response behavior...
  35. pmc Microelectrodes for studying neurobiology
    Justin M Kita
    Department of Chemistry and Neuroscience Center, University of North Carolina, Chapel Hill, NC 27599 3290, USA
    Curr Opin Chem Biol 12:491-6. 2008
    ..Here we restrict our review to microelectrodes that have been used as chemical sensors. They have played a major role in many important neurobiological findings...
  36. doi request reprint Dopamine detection with fast-scan cyclic voltammetry used with analog background subtraction
    Andre Hermans
    Department of Chemistry, University of North Carolina at Chapel Hill, CB 3290, Venable Hall, Chapel Hill, North Carolina 27599 3290, USA
    Anal Chem 80:4040-8. 2008
    ....
  37. pmc Dynamic changes in accumbens dopamine correlate with learning during intracranial self-stimulation
    Catarina A Owesson-White
    Department of Psychology, University of North Carolina, Chapel Hill, NC 27599 3290, USA
    Proc Natl Acad Sci U S A 105:11957-62. 2008
    ..The results show the dynamic nature of chemical signaling in the NAc during ICSS and provide new insight into the role of NAc dopamine in reward-related behaviors...
  38. pmc Basolateral amygdala modulates terminal dopamine release in the nucleus accumbens and conditioned responding
    JOSHUA L JONES
    Department of Psychology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Biol Psychiatry 67:737-44. 2010
    ..However, the role of associative neural substrates such as the basolateral amygdala (BLA) in regulating phasic dopamine release in the NAc, particularly during reward seeking, remains unknown...
  39. ncbi request reprint Neurochemistry and electroanalytical probes
    Kevin P Troyer
    Department of Chemistry, University of North Carolina at Chapel Hill, CB 3290, Venable Hall, 27599, USA
    Curr Opin Chem Biol 6:696-703. 2002
    ..Transgenic mice models have been developed and studied to identify specific cell types in vitro. Characterization and surface modification of electroanalytical probes has enhanced the selectivity and sensitivity of measurements...
  40. ncbi request reprint Dopamine transport into a single cell in a picoliter vial
    Kevin P Troyer
    University of North Carolina at Chapel Hill, Department of Chemistry, 27599, USA
    Anal Chem 74:5370-5. 2002
    ....
  41. pmc Phasic nucleus accumbens dopamine release encodes effort- and delay-related costs
    Jeremy J Day
    Department of Psychology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA
    Biol Psychiatry 68:306-9. 2010
    ..Dopamine transmission within the nucleus accumbens (NAc) has been heavily implicated in reward-learning and decision-making, but it is unclear how dopamine release might contribute to decisions that involve costs...
  42. pmc Vesicular Ca(2+) -induced secretion promoted by intracellular pH-gradient disruption
    Christy L Haynes
    University of North Carolina, Chapel Hill, Department of Chemistry, Venable Hall, Chapel Hill, NC 27599, USA
    Biophys Chem 123:20-4. 2006
    ..In conjunction with previous studies, this work reveals that catalytic and self-sustained vesicular Ca(2+) -induced exocytosis occurs with extended exposure to weak acid or base protonophores...
  43. pmc Carbon-fiber microelectrodes modified with 4-sulfobenzene have increased sensitivity and selectivity for catecholamines
    Andre Hermans
    Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 3290, USA
    Langmuir 22:1964-9. 2006
    ..However, this layer remains permeable to negatively charged compounds. Modified electrodes retain the increased sensitivity for dopamine during measurements in mouse brain tissue...
  44. ncbi request reprint Subsecond dopamine release promotes cocaine seeking
    Paul E M Phillips
    Department of Psychology, Neuroscience Center, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    Nature 422:614-8. 2003
    ..These findings reveal an unprecedented role for dopamine in the regulation of drug taking in real time...
  45. pmc Real-time measurement of dopamine fluctuations after cocaine in the brain of behaving rats
    Michael L A V Heien
    Department of Chemistry and Neuroscience Center, University of North Carolina, Chapel Hill, NC 27599 3290, USA
    Proc Natl Acad Sci U S A 102:10023-8. 2005
    ..Using this method, we show that cocaine modifies dopamine release in two ways: dopamine concentration transients increase in frequency and magnitude, whereas a gradual increase in steady-state dopamine concentration occurs over 90 s...
  46. ncbi request reprint Acute ethanol decreases dopamine transporter velocity in rat striatum: in vivo and in vitro electrochemical measurements
    Donita L Robinson
    Department of Chemistry, Center for Alcohol Studies, University of North Carolina, Chapel Hill, North Carolina 27599 3290, USA
    Alcohol Clin Exp Res 29:746-55. 2005
    ..The present experiments examined the effect of acute ethanol on dopamine transmission, particularly DAT velocity, in anesthetized animals as well as rat striatal suspensions...
  47. pmc Facilitation of quantal release induced by a D1-like receptor on bovine chromaffin cells
    Melissa Villanueva
    Department of Chemistry, The University of North Carolina, Chapel Hill, North Carolina 27599 3290, USA
    Biochemistry 46:3881-7. 2007
    ..Taken together, the data indicate that released catecholamines can enhance their own release by interaction with a D1-like receptor on bovine adrenal chromaffin cells...
  48. ncbi request reprint Paradoxical modulation of short-term facilitation of dopamine release by dopamine autoreceptors
    Justin M Kita
    Department of Chemistry and Neuroscience Center, University of North Carolina, Chapel Hill, North Carolina, USA
    J Neurochem 102:1115-24. 2007
    ..These data demonstrate that the commanding control exerted by dopamine autoreceptors over short-term neuroadaptation of dopamine release involves both inhibitory and paradoxically, facilitatory components...
  49. ncbi request reprint Response times of carbon fiber microelectrodes to dynamic changes in catecholamine concentration
    B Jill Venton
    Department of Chemistry, University of North Carolina, Chapel Hill 27599 3290, USA
    Anal Chem 74:539-46. 2002
    ....
  50. ncbi request reprint Catecholamine exocytosis is diminished in R6/2 Huntington's disease model mice
    Michael A Johnson
    Department of Chemistry The University of North Carolina, Chapel Hill, North Carolina, USA
    J Neurochem 103:2102-10. 2007
    ..Thus, catecholamine release from tissues isolated from R6/2 mice is diminished because of impaired vesicle loading...
  51. ncbi request reprint Overoxidation of carbon-fiber microelectrodes enhances dopamine adsorption and increases sensitivity
    Michael L A V Heien
    Department of Chemistry and Neuroscience Center, Chapel Hill, NC 27599, USA
    Analyst 128:1413-9. 2003
    ..other neurochemicals, and a change in their cyclic voltammograms. Stimulated release in the caudate-putamen was pharmacologically characterized in vivo using Ro-04-1284 and pargyline, and was consistent with that expected for dopamine...
  52. pmc Microfabricated FSCV-compatible microelectrode array for real-time monitoring of heterogeneous dopamine release
    Matthew K Zachek
    Joint Department of Biomedical Engineering, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA
    Analyst 135:1556-63. 2010
    ..The heterogeneous effects of pharmacology on the striatum were also observed and shown to be consistent across multiple animals...
  53. ncbi request reprint Synapsin II negatively regulates catecholamine release
    Melissa Villanueva
    Department of Chemistry, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Brain Cell Biol 35:125-36. 2006
    ....
  54. ncbi request reprint Resolving neurotransmitters detected by fast-scan cyclic voltammetry
    Michael L A V Heien
    Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 3290, USA
    Anal Chem 76:5697-704. 2004
    ..Cells that secreted one or the other were identified. Overall, the results show that principal component regression with appropriate calibration data allows resolution of substances that give overlapping cyclic voltammograms...
  55. pmc Conical tungsten tips as substrates for the preparation of ultramicroelectrodes
    Andre Hermans
    Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 3290, USA
    Langmuir 22:10348-53. 2006
    ..All three types of ultramicroelectrodes fabricated on the tungsten wire had similar electrochemical behavior to electrodes fabricated from wires or fibers insulated with glass tubes...
  56. pmc Simultaneous monitoring of dopamine concentration at spatially different brain locations in vivo
    Matthew K Zachek
    Joint Department of Biomedical Engineering, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA
    Biosens Bioelectron 25:1179-85. 2010
    ....
  57. doi request reprint Amperometry and cyclic voltammetry with carbon fiber microelectrodes at single cells
    Michelle L Mundroff
    The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA
    Curr Protoc Neurosci . 2002
    ....
  58. pmc Monitoring rapid chemical communication in the brain
    Donita L Robinson
    Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 3290, USA
    Chem Rev 108:2554-84. 2008
  59. pmc Fluorinated xerogel-derived microelectrodes for amperometric nitric oxide sensing
    Jae Ho Shin
    Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Anal Chem 80:6850-9. 2008
    ..In addition, the sensor proved functional up to 20 d, maintaining >or=90% of the sensor's initial sensitivity without serious deterioration in selectivity...
  60. ncbi request reprint Phasic dopamine release evoked by abused substances requires cannabinoid receptor activation
    Joseph F Cheer
    Department of Chemistry and Neuroscience Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 3290, USA
    J Neurosci 27:791-5. 2007
    ..These time-resolved chemical measurements provide unique insight into the neurobiological effectiveness of rimonabant in treating addictive disorders...
  61. pmc Electroosmotic flow and its contribution to iontophoretic delivery
    Natalie R Herr
    Department of Chemistry and Neuroscience Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 3290, USA
    Anal Chem 80:8635-41. 2008
    ..With this information, quantitative iontophoresis is possible for electrochemically inactive drugs by using NPE as a marker for EOF...
  62. ncbi request reprint Pharmacologically induced, subsecond dopamine transients in the caudate-putamen of the anesthetized rat
    B Jill Venton
    Department of Chemistry and Neuroscience Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 3290, USA
    Synapse 61:37-9. 2007
    ..The results show that both D2 autoreceptors and the dopamine transporter regulate subsecond DA neurotransmission...
  63. ncbi request reprint Cannabinoid modulation of electrically evoked pH and oxygen transients in the nucleus accumbens of awake rats
    Joseph F Cheer
    Department of Chemistry and Neuroscience Center, The University of North Carolina, Chapel Hill, North Carolina 27599 3290, USA
    J Neurochem 97:1145-54. 2006
    ..Although endogenous cannabinoid tone alone is not sufficient to modify these responses, uptake blockade demonstrates that the system has the potential to exert CB1-specific effects similar to those of full agonists...
  64. ncbi request reprint Nomifensine amplifies subsecond dopamine signals in the ventral striatum of freely-moving rats
    Donita L Robinson
    Department of Chemistry, Center for Alcohol Studies and Neuroscience Center, University of North Carolina, Chapel Hill, North Carolina 27599 3290, USA
    J Neurochem 90:894-903. 2004
    ..These findings demonstrate the heterogeneity of dopamine transients in the ventral striatum and establish that nomifensine elevates the tone of rapid dopamine signals in the brain...
  65. ncbi request reprint Paying attention with the latest technology
    Parastoo Hashemi
    Department of Chemistry and Neuroscience Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 3290, USA
    Neuron 56:4-5. 2007
    ..They show that cue-evoked cholinergic activity in the medial prefrontal cortex is associated with cue detection and not reward delivery. We discuss the implications of their research in behavioral neuroscience...

Research Grants50

  1. DYNAMICS OF IN VIVO DOPAMINE RELEASE
    Robert Wightman; Fiscal Year: 2009
    ..Both targets have dynamic concentration fluctuations in regions of the brain that use DA. Their detection will enable a more complete view of the chemical changes associated with reward. ..
  2. DYNAMICS OF IN VIVO DOPAMINE RELEASE
    Robert Wightman; Fiscal Year: 2006
    ..3) factors mediating the attenuation of phasic DA during intracranial self-stimulation. Collectively, these experiments will provide important insight into the role of phasic DA in reinforcement-related processing. ..
  3. ELECTROANALYSIS OF NEUROTRANSMITTERS AND MODULATORS
    Robert Wightman; Fiscal Year: 2005
    ..02 levels are related to local blood flow. In this work, we plan to probe the relation between local pH and these other two indices. Our hypothesis is that these measures provide a general view of neuronal activation. ..
  4. ELECTROANALYSIS OF NEUROTRANSMITTERS AND MODULATORS
    Robert Wightman; Fiscal Year: 2007
    ..The proposed design uses various forms of carbon deposited on the tips of tungsten wires. These electrodes will facilitate measurements in behaving animals and the development of arrays of electrodes for in vivo use. ..
  5. SECRETION FROM INDIVIDUAL VESICLES
    Robert Wightman; Fiscal Year: 2007
    ..Using visualized dopamine neurons, we will test the hypothesis that the regulation of release is similar in the two different parts of the neuron. ..
  6. DYNAMICS OF IN VIVO DOPAMINE RELEASE
    Robert Wightman; Fiscal Year: 2007
    ..Both targets have dynamic concentration fluctuations in regions of the brain that use DA. Their detection will enable a more complete view of the chemical changes associated with reward. ..
  7. ELECTROANALYSIS OF NEUROTRANSMITTERS AND MODULATORS
    ROBERT MARK WIGHTMAN; Fiscal Year: 2010
    ..The information they provide will enable a subsecond view into their concentration fluctuations of these two neurotransmitter as they regulate specific behaviors. ..
  8. DYNAMICS OF IN VIVO DOPAMINE RELEASE
    ROBERT MARK WIGHTMAN; Fiscal Year: 2010
    ..Both targets have dynamic concentration fluctuations in regions of the brain that use DA. Their detection will enable a more complete view of the chemical changes associated with reward. ..
  9. SECRETION FROM INDIVIDUAL VESICLES
    ROBERT MARK WIGHTMAN; Fiscal Year: 2010
    ..In the proposed research, these processes will be investigated with chemical sensing microelectrodes placed adjacent to isolated cells or implanted in slices of brain tissue. ..
  10. DYNAMICS OF IN VIVO DOPAMINE RELEASE
    Robert Wightman; Fiscal Year: 2009
    ..Both targets have dynamic concentration fluctuations in regions of the brain that use DA. Their detection will enable a more complete view of the chemical changes associated with reward. ..
  11. SECRETION FROM INDIVIDUAL VESICLES
    Robert Wightman; Fiscal Year: 2009
    ..In the proposed research, these processes will be investigated with chemical sensing microelectrodes placed adjacent to isolated cells or implanted in slices of brain tissue. ..
  12. ELECTROANALYSIS OF NEUROTRANSMITTERS AND MODULATORS
    Robert Wightman; Fiscal Year: 2009
    ..The proposed design uses various forms of carbon deposited on the tips of tungstem wires. These electrodes will facilitate measurements in behaving animals and the development of arrays of electrodes for in vivo use. ..
  13. SECRETION FROM INDIVIDUAL VESICLES
    Robert Wightman; Fiscal Year: 2002
    ..From such primary cultures, a clear view on the factors that affect exocytotic release will be obtained. To characterize dopamine uptake at the single cell level. Microsampling techniques will be adapted for this application. ..
  14. DYNAMICS OF IN VIVO DOPAMINE RELEASE
    Robert Wightman; Fiscal Year: 2001
    ..The experiments with the animals with knock-outs of the dopamine transporter will examine the effect of this genetic alteration in the intact animal. ..
  15. ELECTROANALYSIS OF NEUROTRANSMITTERS AND MODULATORS
    Robert Wightman; Fiscal Year: 2000
    ..hydroxytryptamine (5-HT), another biogenic amine neurotransmitter, and will develop new types of chemical sensors specifically designed for the detection of chemical communication between cells. ..
  16. ELECTROANALYSIS OF NEUROTRANSMITTERS AND MODULATORS
    Robert Wightman; Fiscal Year: 1993
    ..In this way the factors which regulate the actions of 5-hydroxytryptamine will be probed...