Michael Wiener

Summary

Affiliation: University of Virginia
Country: USA

Publications

  1. ncbi request reprint A pedestrian guide to membrane protein crystallization
    Michael C Wiener
    Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, VA 22908 0736, USA
    Methods 34:364-72. 2004
  2. pmc Crystallization and preliminary X-ray crystallographic analysis of the Escherichia coli outer membrane cobalamin transporter BtuB in complex with the carboxy-terminal domain of TonB
    David D Shultis
    Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, Virginia 22908, USA
    Acta Crystallogr Sect F Struct Biol Cryst Commun 62:638-41. 2006
  3. pmc Mesoscopic surfactant organization and membrane protein crystallization
    M C Wiener
    Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville 22908 0736, USA
    Protein Sci 9:1407-9. 2000
  4. ncbi request reprint Bacterial export takes its Tol
    M C Wiener
    Department of Molecular Physiology and Biological Physics, University of Virginia, PO Box 800736, Charlottesville, VA 22908 0736, USA
    Structure 8:R171-5. 2000
  5. pmc When worlds colloid
    Michael C Wiener
    Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, Virginia 22908 0736, USA
    Protein Sci 15:2679-81. 2006
  6. ncbi request reprint TonB-dependent outer membrane transport: going for Baroque?
    Michael C Wiener
    Department of Molecular Physiology and Biological Physics, University of Virginia, PO Box 800736, Charlottesville, VA 22908 0736, USA
    Curr Opin Struct Biol 15:394-400. 2005
  7. ncbi request reprint Comparative structural analysis of TonB-dependent outer membrane transporters: implications for the transport cycle
    David P Chimento
    Department of Microbiology, University of Virginia, Charlottesville, Virginia, USA
    Proteins 59:240-51. 2005
  8. ncbi request reprint Outer membrane active transport: structure of the BtuB:TonB complex
    David D Shultis
    Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, VA 22908, USA
    Science 312:1396-9. 2006
  9. pmc Conformational exchange in a membrane transport protein is altered in protein crystals
    Daniel M Freed
    Departments of Chemistry, University of Virginia, Charlottesville, Virginia, USA
    Biophys J 99:1604-10. 2010
  10. ncbi request reprint The Escherichia coli outer membrane cobalamin transporter BtuB: structural analysis of calcium and substrate binding, and identification of orthologous transporters by sequence/structure conservation
    David P Chimento
    Department of Microbiology, University of Virginia, Charlottesville, VA 22908 0734, USA
    J Mol Biol 332:999-1014. 2003

Collaborators

  • WILLIAM WIMLEY
  • Genji Kurisu
  • Stanislav D Zakharov
  • WILLIAM CRAMER
  • Arun K Mohanty
  • David P Chimento
  • Robert J Kadner
  • David D Shultis
  • Daniel M Freed
  • James Gumbart
  • Michael D Purdy
  • Christian N Banchs
  • Nathalie Cadieux
  • Peter S Horanyi
  • David S Cafiso
  • Emad Tajkhorshid
  • Thomas C Bishop
  • Christopher M Bishop
  • Chad R Simmons
  • Eva Reeger-Schneider
  • Wolfgang Koster
  • Clive Bradbeer

Detail Information

Publications18

  1. ncbi request reprint A pedestrian guide to membrane protein crystallization
    Michael C Wiener
    Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, VA 22908 0736, USA
    Methods 34:364-72. 2004
    ..The major difference, and a non-trivial difference, is the necessity for inclusion of detergents above the critical micelle concentration in the purified membrane protein solution...
  2. pmc Crystallization and preliminary X-ray crystallographic analysis of the Escherichia coli outer membrane cobalamin transporter BtuB in complex with the carboxy-terminal domain of TonB
    David D Shultis
    Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, Virginia 22908, USA
    Acta Crystallogr Sect F Struct Biol Cryst Commun 62:638-41. 2006
    ..3, b = 82.4, c = 122.6 angstroms). The asymmetric unit consists of a single BtuB-TonB complex. Data sets have been collected to 2.1 angstroms resolution at a synchrotron beamline (APS SER-CAT 22-ID)...
  3. pmc Mesoscopic surfactant organization and membrane protein crystallization
    M C Wiener
    Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville 22908 0736, USA
    Protein Sci 9:1407-9. 2000
    ..Well-ordered crystals of the water channel human aquaporin-1 (hAQP1) that diffract to 4 A resolution have been obtained with this approach...
  4. ncbi request reprint Bacterial export takes its Tol
    M C Wiener
    Department of Molecular Physiology and Biological Physics, University of Virginia, PO Box 800736, Charlottesville, VA 22908 0736, USA
    Structure 8:R171-5. 2000
    ..The structure is compared to those of other proteins that are embedded in bacterial outer membranes or that traverse the periplasmic space...
  5. pmc When worlds colloid
    Michael C Wiener
    Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, Virginia 22908 0736, USA
    Protein Sci 15:2679-81. 2006
  6. ncbi request reprint TonB-dependent outer membrane transport: going for Baroque?
    Michael C Wiener
    Department of Molecular Physiology and Biological Physics, University of Virginia, PO Box 800736, Charlottesville, VA 22908 0736, USA
    Curr Opin Struct Biol 15:394-400. 2005
    ..The reconciliation of structural and non-structural experimental data, and the unambiguous experimental elucidation of a detailed molecular mechanism of transport are current challenges for this field...
  7. ncbi request reprint Comparative structural analysis of TonB-dependent outer membrane transporters: implications for the transport cycle
    David P Chimento
    Department of Microbiology, University of Virginia, Charlottesville, Virginia, USA
    Proteins 59:240-51. 2005
    ..Also, these structural changes may require only modest forces exerted by the energy-coupling TonB protein upon the transporter...
  8. ncbi request reprint Outer membrane active transport: structure of the BtuB:TonB complex
    David D Shultis
    Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, VA 22908, USA
    Science 312:1396-9. 2006
    ..In contrast to its conformations in the absence of TonB, the Ton-box forms a beta strand that is recruited to the existing beta sheet of TonB, which is consistent with a mechanical pulling model of transport...
  9. pmc Conformational exchange in a membrane transport protein is altered in protein crystals
    Daniel M Freed
    Departments of Chemistry, University of Virginia, Charlottesville, Virginia, USA
    Biophys J 99:1604-10. 2010
    ..These data provide a quantitative measure of how a conformational equilibrium in BtuB is modified in the crystal environment, and suggest that more-compact, less-hydrated substates will be favored in protein crystals...
  10. ncbi request reprint The Escherichia coli outer membrane cobalamin transporter BtuB: structural analysis of calcium and substrate binding, and identification of orthologous transporters by sequence/structure conservation
    David P Chimento
    Department of Microbiology, University of Virginia, Charlottesville, VA 22908 0734, USA
    J Mol Biol 332:999-1014. 2003
    ....
  11. ncbi request reprint Enzymatic E-colicins bind to their target receptor BtuB by presentation of a small binding epitope on a coiled-coil scaffold
    Arun K Mohanty
    Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, Virginia 22908 0736, USA
    J Biol Chem 278:40953-8. 2003
    ..We conclude that the BtuB binding sites for cobalamins and enzymatic E-colicins are overlapping but inequivalent and that the distal loop and (possibly) the short alpha-helical flanking regions are sufficient for high affinity binding...
  12. ncbi request reprint Substrate-induced transmembrane signaling in the cobalamin transporter BtuB
    David P Chimento
    Department of Microbiology, University of Virginia, Charlottesville, Virginia 22908, USA
    Nat Struct Biol 10:394-401. 2003
    ..3 nM) of cyanocobalamin to BtuB. We observe two bound calcium ions that order three extracellular loops of BtuB, thus providing a direct (and unusual) structural role for calcium...
  13. ncbi request reprint Crystallization and initial X-ray diffraction of BtuB, the integral membrane cobalamin transporter of Escherichia coli
    David P Chimento
    Department of Microbiology, University of Virginia, Charlottesville, Virginia 22908, USA
    Acta Crystallogr D Biol Crystallogr 59:509-11. 2003
    ..Each crystal form contains a monomer in the asymmetric unit. Diffraction for crystal type I extends to 2.0 A and diffraction for crystal type II extends to 2.7 A. Both crystal forms are suitable for structure determination...
  14. pmc Identification of the periplasmic cobalamin-binding protein BtuF of Escherichia coli
    Nathalie Cadieux
    Department of Microbiology, School of Medicine, University of Virginia, Charlottesville, Virginia 22908 0734, USA
    J Bacteriol 184:706-17. 2002
    ..These results finally establish the identity of the periplasmic binding protein for Cbl uptake, which is one of few cases where the components of a periplasmic permease are genetically separated...
  15. ncbi request reprint Membrane protein expression and production: effects of polyhistidine tag length and position
    Arun K Mohanty
    Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, VA 22908 0736, USA
    Protein Expr Purif 33:311-25. 2004
    ..We are currently producing approximately 12 mg of purified AqpZ per liter of shake-flask culture, and preliminary crystals that diffract to approximately 5A resolution have been obtained...
  16. ncbi request reprint Inhibition of tobacco etch virus protease activity by detergents
    Arun K Mohanty
    Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, VA 22908 0736, USA
    Protein Expr Purif 27:109-14. 2003
    ..Our data indicate that half of the detergents tested reduce the activity of rTEV and that these detergents should be avoided or otherwise accounted for during rTEV digestion of recombinant integral membrane proteins...
  17. pmc Mechanics of force propagation in TonB-dependent outer membrane transport
    James Gumbart
    Department of Physics and Beckman Institute, University of Illinois at Urbana Champaign, Urbana, Illinois, USA
    Biophys J 93:496-504. 2007
    ..Furthermore, analysis of the electrostatic potentials and salt bridge interactions between the two proteins during the simulations hints at involvement of electrostatic forces in long-range interaction and binding of TonB and BtuB...
  18. ncbi request reprint The structure of BtuB with bound colicin E3 R-domain implies a translocon
    Genji Kurisu
    Department of Biological Sciences, Purdue University, Lilly Hall of Life Sciences, 915 W State St, West Lafayette, Indiana 47907 1392, USA
    Nat Struct Biol 10:948-54. 2003
    ..The translocator was concluded to be OmpF from the occlusion of OmpF channels by colicin E3...

Research Grants23

  1. PERIPHERAL BENZODIAZEPINE RECEPTOR STRUCTURAL BIOLOGY
    Michael Wiener; Fiscal Year: 2006
    ....
  2. Improved Methods for Membrane Protein Crystallization(RMI)
    Michael Wiener; Fiscal Year: 2007
    ..Other planned targets will focus upon other human membrane proteins of fundamental and biomedical import. ..
  3. Structural Biology of Cancer Related Membrane Proteins Expressed in P. Pastoris
    Michael Wiener; Fiscal Year: 2007
    ..We will use an automated plate imager to view and document crystallization experiments. We will solve the structures of CRIMPs that form high quality crystals. ..
  4. TonB-Dependent Outer Membrane Transport
    Michael Wiener; Fiscal Year: 2009
    ..This research is directed towards understanding how a portion of this transport pathway works, and a long-range goal of this research is to develop antibacterial drugs targeted to this transport pathway. ..
  5. Improved Methods for Membrane Protein Crystallization
    Michael Wiener; Fiscal Year: 2009
    ..Other planned targets will focus upon other human membrane proteins of fundamental and biomedical import. ..
  6. Structural Biology of Cancer Related Membrane Proteins Expressed in P. Pastoris
    Michael Wiener; Fiscal Year: 2009
    ..We will use an automated plate imagerto view and document crystallization experiments. We will solve the structures of CRIMPs that form high quality crystals. ..
  7. STRUCTURAL BIOLOGY OF THE HUMAN COPPER TRANSPORTER CTR1
    Michael Wiener; Fiscal Year: 2005
    ..abstract_text> ..
  8. X RAY CRYSTALLOGRAPHY OF HUMAN AQUAPORIN WATER CHANNELS
    Michael Wiener; Fiscal Year: 2001
    ..Lastly, recombinant aquaporins other than AQP-1 will be produced for crystallization experiments. The ultimate goal is the determination of an ensemble of human aquaporin structures for use in structure- based drug design. ..
  9. STRUCTURAL BIOLOGY OF BACTERIAL COBALAMIN TRANSPORT
    Michael Wiener; Fiscal Year: 2005
    ..The nature of these interactions will be investigated by making, crystallizing and solving complexes of receptor-binding domains of E colicins and BtuB. ..
  10. TonB-Dependent Outer Membrane Transport
    Michael Wiener; Fiscal Year: 2010
    ..This research is directed towards understanding how a portion of this transport pathway works, and a long-range goal of this research is to develop antibacterial drugs targeted to this transport pathway. ..