Jonathan H Widdicombe

Summary

Affiliation: University of California
Country: USA

Publications

  1. pmc Chloride secretion by cultures of pig tracheal gland cells
    J H Widdicombe
    Department of Physiology and Membrane Biology, University of California Davis, CA 95616 8664, USA
    Am J Physiol Lung Cell Mol Physiol 302:L1098-106. 2012
  2. ncbi request reprint Secretion of lactoferrin and lysozyme by cultures of human airway epithelium
    R F Dubin
    Dept of Human Physiology, Univ of California Davis, Davis, CA 95616 8664, USA
    Am J Physiol Lung Cell Mol Physiol 286:L750-5. 2004
  3. ncbi request reprint Effects of growth surface on differentiation of cultures of human tracheal epithelium
    J H Widdicombe
    Department of Human Physiology, University of California Davis, Davis, California 95616, USA
    In Vitro Cell Dev Biol Anim 39:51-5. 2003
  4. pmc Interleukin-13-induced mucous metaplasia increases susceptibility of human airway epithelium to rhinovirus infection
    Marrah E Lachowicz-Scroggins
    Department of Physiology and Membrane Biology, School of Medicine, University of California at Davis, One Shields Avenue, Davis, CA 95616 8643, USA
    Am J Respir Cell Mol Biol 43:652-61. 2010
  5. pmc Regulation of the depth and composition of airway surface liquid
    J H Widdicombe
    Department of Human Physiology, University of California Davis, 95616 8664, USA
    J Anat 201:313-8. 2002
  6. pmc Transgenic animals may help resolve a sticky situation in cystic fibrosis
    Jonathan H Widdicombe
    Department of Physiology and Membrane Biology, University of California Davis, Davis, California 95616 8664, USA
    J Clin Invest 120:3093-6. 2010
  7. ncbi request reprint Expansion of cultures of human tracheal epithelium with maintenance of differentiated structure and function
    Jonathan H Widdicombe
    Department of Human Physiology, University of California Davis, Davis, CA 95616 8664, USA
    Biotechniques 39:249-55. 2005
  8. pmc Distribution of tracheal and laryngeal mucous glands in some rodents and the rabbit
    J H Widdicombe
    Children s Hospital Oakland Research Institute, CA, USA
    J Anat 198:207-21. 2001
  9. ncbi request reprint Altered NaCl concentration of airway surface liquid in cystic fibrosis
    J H Widdicombe
    Department of Human Physiology, University of California Davis, Davis, CA 95616 8664, USA
    Pflugers Arch 443:S8-10. 2001
  10. pmc CFTR and calcium-activated chloride channels in primary cultures of human airway gland cells of serous or mucous phenotype
    Horst Fischer
    Children s Hospital Oakland Research Institute, Oakland, California, USA
    Am J Physiol Lung Cell Mol Physiol 299:L585-94. 2010

Collaborators

Detail Information

Publications32

  1. pmc Chloride secretion by cultures of pig tracheal gland cells
    J H Widdicombe
    Department of Physiology and Membrane Biology, University of California Davis, CA 95616 8664, USA
    Am J Physiol Lung Cell Mol Physiol 302:L1098-106. 2012
    ..They are, therefore, potentially useful in determining if CF-related alterations in anion transport result in altered secretion of serous cell antimicrobial agents or mucus...
  2. ncbi request reprint Secretion of lactoferrin and lysozyme by cultures of human airway epithelium
    R F Dubin
    Dept of Human Physiology, Univ of California Davis, Davis, CA 95616 8664, USA
    Am J Physiol Lung Cell Mol Physiol 286:L750-5. 2004
    ..Thus our data cast doubt on the utility of Calu-3 cells as a model of airway serous gland cells but do suggest that HTE could prove highly suitable for studies of mucin synthesis and release...
  3. ncbi request reprint Effects of growth surface on differentiation of cultures of human tracheal epithelium
    J H Widdicombe
    Department of Human Physiology, University of California Davis, Davis, California 95616, USA
    In Vitro Cell Dev Biol Anim 39:51-5. 2003
    ..Thus, it is both possible and desirable to make all functional measurements on cells grown on clear porous supports...
  4. pmc Interleukin-13-induced mucous metaplasia increases susceptibility of human airway epithelium to rhinovirus infection
    Marrah E Lachowicz-Scroggins
    Department of Physiology and Membrane Biology, School of Medicine, University of California at Davis, One Shields Avenue, Davis, CA 95616 8643, USA
    Am J Respir Cell Mol Biol 43:652-61. 2010
    ..We conclude that mucous metaplasia in asthma increases the susceptibility of airway epithelium to infection by rhinovirus because of changes in the overall architecture of the apical surface...
  5. pmc Regulation of the depth and composition of airway surface liquid
    J H Widdicombe
    Department of Human Physiology, University of California Davis, 95616 8664, USA
    J Anat 201:313-8. 2002
    ..Altered salt and water transport by the surface epithelium may also contribute to the pathology of cystic fibrosis...
  6. pmc Transgenic animals may help resolve a sticky situation in cystic fibrosis
    Jonathan H Widdicombe
    Department of Physiology and Membrane Biology, University of California Davis, Davis, California 95616 8664, USA
    J Clin Invest 120:3093-6. 2010
    ..It is expected, therefore, that these animals will develop lung disease similar to human CF and prove to be valuable models on which to test potential therapies...
  7. ncbi request reprint Expansion of cultures of human tracheal epithelium with maintenance of differentiated structure and function
    Jonathan H Widdicombe
    Department of Human Physiology, University of California Davis, Davis, CA 95616 8664, USA
    Biotechniques 39:249-55. 2005
    ..However, there was a marked decline in differentiation by cells passaged three times (P3), and not all cell preparations could be taken to P4 (cells passaged four times)...
  8. pmc Distribution of tracheal and laryngeal mucous glands in some rodents and the rabbit
    J H Widdicombe
    Children s Hospital Oakland Research Institute, CA, USA
    J Anat 198:207-21. 2001
    ..These may correspond to the 'nests' of goblet cells described by others...
  9. ncbi request reprint Altered NaCl concentration of airway surface liquid in cystic fibrosis
    J H Widdicombe
    Department of Human Physiology, University of California Davis, Davis, CA 95616 8664, USA
    Pflugers Arch 443:S8-10. 2001
    ....
  10. pmc CFTR and calcium-activated chloride channels in primary cultures of human airway gland cells of serous or mucous phenotype
    Horst Fischer
    Children s Hospital Oakland Research Institute, Oakland, California, USA
    Am J Physiol Lung Cell Mol Physiol 299:L585-94. 2010
    ....
  11. pmc Cystic fibrosis and the relationship between mucin and chloride secretion by cultures of human airway gland mucous cells
    Walter E Finkbeiner
    Department of Pathology, University of California, San Francisco, 94110, USA
    Am J Physiol Lung Cell Mol Physiol 301:L402-14. 2011
    ....
  12. ncbi request reprint Basolateral Cl channels in primary airway epithelial cultures
    Horst Fischer
    Children s Hospital Oakland Research Institute, 5700 Martin Luther King, Jr Way, Oakland, CA 94609, USA
    Am J Physiol Lung Cell Mol Physiol 292:L1432-43. 2007
    ..We propose that, depending on the driving force for Cl across the apical membrane, basolateral Cl channels confine Cl(-) secretion or support transcellular Cl(-) absorption...
  13. pmc Cultures of human tracheal gland cells of mucous or serous phenotype
    Walter E Finkbeiner
    Department of Pathology, University of California, San Francisco, CA, USA
    In Vitro Cell Dev Biol Anim 46:450-6. 2010
    ..Measurements of transepithelial resistance and short-circuit current showed that both "serous" and "mucous" cell cultures possessed tight junctions, had become polarized, and were actively secreting Cl...
  14. pmc In vitro susceptibility to rhinovirus infection is greater for bronchial than for nasal airway epithelial cells in human subjects
    Nilceia Lopez-Souza
    Department of Medicine, University of California, San Francisco, Calif, USA
    J Allergy Clin Immunol 123:1384-90.e2. 2009
    ..Human rhinoviruses (HRVs) characteristically cause upper respiratory tract infection, but they also infect the lower airways, causing acute bronchitis and exacerbating asthma...
  15. ncbi request reprint Effects of media on differentiation of cultured human tracheal epithelium
    L A Sachs
    Department of Human Physiology, University of California Davis, Davis, California 95616, USA
    In Vitro Cell Dev Biol Anim 39:56-62. 2003
    ..In contrast, the two media with the lowest [Ca] (0.11 mM) produced poorly differentiated cells, as did the two partially defined media with low or no retinoic acid concentration...
  16. pmc Mechanisms of acid and base secretion by the airway epithelium
    Horst Fischer
    Children s Hospital Oakland Research Institute, Oakland, CA 94609, USA
    J Membr Biol 211:139-50. 2006
    ..Current knowledge of acid and base transporters and their potential roles in airway mucosal pH regulation is reviewed here...
  17. ncbi request reprint Acid secretion and proton conductance in human airway epithelium
    Horst Fischer
    Children s Hospital Oakland Research Institute, 5700 Martin Luther King Jr Way, Oakland, CA 94609, USA
    Am J Physiol Cell Physiol 282:C736-43. 2002
    ..2 +/- 3.8 pS/pF (at 0 mV and a pH 5.3-to-pH 7.3 gradient). We suggest that inflammatory processes activate proton secretion by the airway epithelium and acidify the airway surface liquid...
  18. doi request reprint Quantitative real-time PCR for rhinovirus, and its use in determining the relationship between TCID50 and the number of viral particles
    Lorne A Sachs
    Department of Physiology and Membrane Biology, University of California Davis, Davis, CA 95616 8643, USA
    J Virol Methods 171:212-8. 2011
    ..This new qPCR will permit quantitative assessments of interactions between virus and epithelium such as determinations of the affinity and number of viral binding sites or of the number of virus produced per infected cell...
  19. ncbi request reprint Endotoxin induces leukocyte transmigration and changes in permeability of the airway epithelium via protein-kinase C and extracellular regulated kinase activation
    Vladimir B Serikov
    Children s Hospital Oakland Research Institute, California 95616, USA
    J Endotoxin Res 10:55-65. 2004
    ..Again, these effects were markedly inhibited by PD98059 and calphostin C (by > 50%). We conclude that epithelial ERK1/2 is activated by endotoxin via PKC and is an important pathway in regulation of epithelial permeability...
  20. ncbi request reprint Differentiated structure and function of primary cultures of monkey oviductal epithelium
    M Rajagopal
    Department of Physiology and Membrane Biology, University of California Davis, CA 95616, USA
    In Vitro Cell Dev Biol Anim 42:248-54. 2006
    ....
  21. ncbi request reprint Regulation of depth and composition of airway surface liquid
    J H Widdicombe
    Children s Hospital Oakland Research Institute, CA 94609, USA
    Eur Respir J 10:2892-7. 1997
    ..Finally, we present preliminary data showing that LT-SEM can be combined with X-ray microanalysis to determine the elemental composition of ASL...
  22. ncbi request reprint Resistance of differentiated human airway epithelium to infection by rhinovirus
    N Lopez-Souza
    Department of Human Physiology, University of California Davis, Davis CA 95616 8664, USA
    Am J Physiol Lung Cell Mol Physiol 286:L373-81. 2004
    ..Thus well-differentiated cells are much more resistant to viral infection and its functional consequences than are poorly differentiated cells from the same source...
  23. ncbi request reprint Distribution and numbers of mucous glands in the horse trachea
    J H Widdicombe
    Department of Human Physiology, University of California Davis, 95616, USA
    Equine Vet J 34:630-3. 2002
    ..Instead, they may be caused mainly by hyperplasia of the mucus-producing cells of the surface epithelium or by vascular transudation...
  24. pmc Alveolar epithelial type I cells contain transport proteins and transport sodium, supporting an active role for type I cells in regulation of lung liquid homeostasis
    Meshell D Johnson
    Department of Medicine, University of California, San Francisco, CA 94143, USA
    Proc Natl Acad Sci U S A 99:1966-71. 2002
    ..These results modify some basic concepts about lung liquid transport, suggesting that TI cells may contribute significantly in maintaining alveolar fluid balance and in resolving airspace edema...
  25. ncbi request reprint Are Drosophila a useful model for understanding the toxicity of inhaled oxidative pollutants: a review
    Malinda Wilson
    Division of Pulmonary Medicine, University of California, Davis, California 95817, USA
    Inhal Toxicol 17:765-74. 2005
    ..We conclude that the insect respiratory system has considerable promise toward understanding novel aspects of vertebrate respiratory tract responses to inhaled oxidative environmental challenges...
  26. ncbi request reprint Estimate of the subepithelial hydrostatic pressure that drives inflammatory transudate into the airway lumen
    V B Serikov
    Department of Human Physiology, University of California Davis, Davis 95616, USA
    J Appl Physiol 92:1702-8. 2002
    ..5 cmH2O, and, to block epithelial transudation induced by leukocyte traffic, it was 3 cmH2O, and we conclude that these are the subepithelial pressures that drive inflammatory transudation into the airway lumen...
  27. ncbi request reprint Polarized cultures of human airway epithelium from nasal scrapings and bronchial brushings
    Nilceia Lopez-Souza
    Division of Medicine, Allergy and Immunology, University of California San Francisco, and Children s Hospital Oakland Research Institute, USA
    In Vitro Cell Dev Biol Anim 39:266-9. 2003
    ..For the first time, we report that human airway epithelial cells obtained from nasal scrapings or bronchial brushings can be grown in culture to produce polarized cell sheets suitable for studies of vectorial transport...
  28. ncbi request reprint Volume of airway surface liquid in health and disease
    Jonathan H Widdicombe
    Am J Respir Crit Care Med 165:1566. 2002
  29. ncbi request reprint Measurement of nasal potential difference in mild asthmatics
    Nancy C Chung
    Department of Pediatric Critical Care, Children s Hospital Oakland, CA 94609, USA
    Chest 123:1467-71. 2003
    ..To determine whether ion transport or barrier function across the nasal epithelium are altered in asthmatics...
  30. ncbi request reprint Activation of extracellular regulated kinases is required for the increase in airway epithelial permeability during leukocyte transmigration
    Vladimir B Serikov
    Children s Hospital Oakland Research Institute, California, USA
    Am J Respir Cell Mol Biol 30:261-70. 2004
    ..We conclude that PKC and ERK1/2 pathways are activated in airway epithelial cells during migration of leukocytes and are important regulators of airway epithelial permeability...
  31. ncbi request reprint Innate immune responses of human tracheal epithelium to Pseudomonas aeruginosa flagellin, TNF-alpha, and IL-1beta
    Jill Tseng
    Dept of Molecular and Cell Biology, 231 LSA, Univ of California Berkeley, Berkeley, CA 94720 3200, USA
    Am J Physiol Cell Physiol 290:C678-90. 2006
    ..4) Exposure of basolateral surfaces to inflammatory activators elicits larger responses because both columnar and basal cells are activated, likely because both cell types express receptors for flagellin, IL-1beta, and TNF-alpha...
  32. ncbi request reprint A novel extract SB-300 from the stem bark latex of Croton lechleri inhibits CFTR-mediated chloride secretion in human colonic epithelial cells
    Horst Fischer
    Children s Hospital Oakland Research Institute, 5700 Martin Luther King Jr Way, Oakland, CA 94609, USA
    J Ethnopharmacol 93:351-7. 2004
    ..SB-300 is a low cost herbal extract and may present a complementary and alternative medicine approach for the treatment of fluid loss in watery diarrhea...