Research Topics
Genomes and Genes | W B WhitmanSummaryAffiliation: University of Georgia Country: USA Publications
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Publications
Development of genetic approaches for the methane-producing archaebacterium Methanococcus maripaludisW B Whitman
Department of Microbiology, University of Georgia, Athens 30602 2605, USA
Biofactors 6:37-46. 1997..Lastly, a shuttle vector has been constructed from a cryptic methanococcal plasmid. These technical advances made it possible to utilize genetic approaches for the study of autotrophic CO2 assimilation in methanococci...
Prokaryotes: the unseen majorityW B Whitman
Department of Microbiology, University of Georgia, Athens GA 30602, USA
Proc Natl Acad Sci U S A 95:6578-83. 1998..7 x 10(30) cells/yr and is highest in the open ocean. The large population size and rapid growth of prokaryotes provides an enormous capacity for genetic diversity...
Characterization of pURB500 from the archaeon Methanococcus maripaludis and construction of a shuttle vectorD L Tumbula
Department of Microbiology, University of Georgia, Athens 30602 2605, USA
J Bacteriol 179:2976-86. 1997..2. Based on the inability of various restriction fragments of pURB500 to support maintenance in M. maripaludis JJ, multiple regions of pURB500 were required. pDLT44 did not replicate in Methanococcus voltae...
Characterization of enzymes of the branched-chain amino acid biosynthetic pathway in Methanococcus sppR Y Xing
Department of Microbiology, University of Georgia, Athens 30602
J Bacteriol 173:2086-92. 1991..In conclusion, the archaebacterial enzymes are functionally homologous to the eubacterial and eucaryotic enzymes, which implies that this pathway is very ancient...
Characterization of guanine and hypoxanthine phosphoribosyltransferases in Methanococcus voltaeT L Bowen
Department of Microbiology, University of Georgia, Athens 30602 2605, USA
J Bacteriol 178:2521-6. 1996..Two mutants resistant to both 8-azaguanine and 8-azahypoxanthine lacked activity for both guanine and hypoxanthine PRTase. These results suggest that analog resistance was acquired by the loss of PRTase activity...
Expression vectors for Methanococcus maripaludis: overexpression of acetohydroxyacid synthase and beta-galactosidaseW L Gardner
Department of Microbiology, University of Georgia, Athens, Georgia 30602 2605, USA
Genetics 152:1439-47. 1999..During this work, the genes for the acetohydroxyacid synthase (ilvBN) and phosphoenolpyruvate synthase (ppsA) were sequenced from a M. maripaludis genomic library...
Relationship of 16S rRNA sequence similarity to DNA hybridization in prokaryotesJ Keswani
Department of Microbiology, University of Georgia, Athens 30602-2605, USA
Int J Syst Evol Microbiol 51:667-78. 2001..For these taxa, S was a poor indicator of relatedness for closely related strains. Thus, the ultrametric properties of rRNA sequences should be tested before making taxonomic or phylogenetic conclusions based upon S...
Cloning and phylogenetic analysis of the genes encoding acetohydroxyacid synthase from the archaeon Methanococcus aeolicusT L Bowen
Department of Microbiology, University of Georgia, Athens 30602 2605, USA
Gene 188:77-84. 1997..One of the ORFs in the Methanococcus jannaschii genome possesses high similarity to the M. aeolicus ilvB, indicating that it is an authentic AHAS...
Quantitative comparisons of 16S rRNA gene sequence libraries from environmental samplesD R Singleton
Department of Microbiology, University of Georgia, Athens, Georgia 30602-2605, USA
Appl Environ Microbiol 67:4374-6. 2001..This method successfully distinguished rRNA gene sequence libraries from soil and bioreactors and correctly failed to find differences between libraries of the same composition...
