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Genomes and Genes | J P WhiteleggeSummaryAffiliation: University of California Country: USA Publications
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Publications
Electrospray-ionization mass spectrometry of intact intrinsic membrane proteinsJ P Whitelegge
Center for Molecular and Medical Sciences Mass Spectrometry, Department of Chemistry and Biochemistry, University of California, Los Angeles 90095 1569, USA
Protein Sci 7:1423-30. 1998..The apparent retention of structure by bacteriorhodopsin during the analysis raises the potential of obtaining tertiary structure information using more developed ESI-MS experiments...
Expression, purification, crystallization, and preliminary X-ray analysis of recombinant human saposin BVictoria E Ahn
Department of Medical Biophysics, University of Toronto, 610 University Avenue, Toronto, Ont, Canada M5G 2M9
Protein Expr Purif 27:186-93. 2003..The three-dimensional structure of saposin B protein will provide insights into how this 79 amino acid protein is able to solubilize relatively large membrane-bound lipid ligands...- Mass spectral analysis of pig (Sus scrofa) apo HDL: Identification of pig apoA-II, a dimeric apolipoproteinDonald L Puppione
The Molecular Biology Institute and The Department of Chemistry and Biochemistry, University of California at Los Angeles, Los Angeles, CA 90095, USA
Comp Biochem Physiol B Biochem Mol Biol 141:89-94. 2005..In addition to being the first to report on the presence of apoA-II in pig plasma, we also obtained values for the molecular masses of apoA-I, apoC-III, apoD and serum amyloid A protein... - Plant peptides and peptidomicsNaser Farrokhi
National Institute of Genetic Engineering and Biotechnology, Pajoohesh Blvd, Tehran Karaj Highway, 17th Km, Tehran, Iran
Plant Biotechnol J 6:105-34. 2008..This review describes known plant peptides and introduces the use of peptidomics for the detection of novel plant peptides... - Initiation and elongation in fibrillation of ALS-linked superoxide dismutaseMadhuri Chattopadhyay
Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095, USA
Proc Natl Acad Sci U S A 105:18663-8. 2008..Our findings provide a rare glimpse into the specific changes in a protein that can lead to nucleation and into the ability of amyloid nuclei to recruit diverse forms of the same protein into fibrils... 
Integral membrane proteins and bilayer proteomicsJulian P Whitelegge
The Pasarow Mass Spectrometry Laboratory, The NPI Semel Institute for Neuroscience and Human Behavior, The David Geffen School of Medicine, University of California, Los Angeles, 760 Westwood Plaza, Los Angeles, California 90095, United States
Anal Chem 85:2558-68. 2013....- Intact protein mass spectrometry and top-down proteomicsJulian Whitelegge
The Pasarow Mass Spectrometry Laboratory, UCLA, Jane and Terry Semel Institute for Neuroscience and Human Behavior, Los Angeles, CA 90095, USA
Expert Rev Proteomics 10:127-9. 2013.... - Full subunit coverage liquid chromatography electrospray ionization mass spectrometry (LCMS+) of an oligomeric membrane protein: cytochrome b(6)f complex from spinach and the cyanobacterium Mastigocladus laminosusJulian P Whitelegge
The Pasarow Mass Spectrometry Laboratory, Department of Psychiatry, Neuropsychiatric Institute, University of California, Los Angeles, California 90095, USA
Mol Cell Proteomics 1:816-27. 2002..Full subunit coverage of an oligomeric intrinsic membrane protein complex by LCMS+ presents a new facet to intact mass proteomics... - Characterization of a recombinant molecule covalently indistinguishable from human cerebroside-sulfate activator protein (CSAct or Saposin B)J P Whitelegge
The Pasarow Mass Spectrometry Laboratory, Department of Psychiatry and Biobehavioral Sciences, The Neuropsychiatric Institute, University of California, 405 Hilgard Ave, Los Angeles, CA 90095, USA
Cell Mol Biol (Noisy-le-grand) 49:799-807. 2003..It is concluded that while glycosylation is unnecessary for in vitro and ex vivo activity of CSAct, modification of the native N21 is necessary to prevent loss of ex vivo activity, possibly via protection from degradation... - HPLC and mass spectrometry of intrinsic membrane proteinsJulian P Whitelegge
Department of Psychiatry and Biobehavioral Sciences, University of California, LosAngeles, CA, USA
Methods Mol Biol 251:323-40. 2004 - Methionine oxidation within the cerebroside-sulfate activator protein (CSAct or Saposin B)J P Whitelegge
Pasarow Mass Spectrometry Laboratory, University of California, Los Angeles 90095, USA
Protein Sci 9:1618-30. 2000..This, in turn, could contribute to the onset and progression of neurodegenerative disease, especially in situations where the catabolism of these materials is marginal... - Subtle modification of isotope ratio proteomics; an integrated strategy for expression proteomicsJulian P Whitelegge
The Pasarow Mass Spectrometry Laboratory, Department of Psychiatry, University of California, Los Angeles, CA 90095, USA
Phytochemistry 65:1507-15. 2004..It is suggested that subtle modification of isotope ratio proteomics (SMIRP) offers a convenient approach to in vivo isotope coding of plants and might ultimately be extended to mammals including humans... - Plant proteomics: BLASTing out of a MudPITJulian P Whitelegge
The Pasarow Mass Spectrometry Laboratory, Department of Psychiatry and Biobehavioral Sciences, and Neuropsychiatric Institute, University of California, 405 Hilgard Avenue, Los Angeles, CA 90095, USA
Proc Natl Acad Sci U S A 99:11564-6. 2002 - Sequencing covalent modifications of membrane proteinsJulian P Whitelegge
The Pasarow Mass Spectrometry Laboratory, and the Jane and Terry Semel Institute for Neuroscience and Human Behavior, David Geffen School of Medicine, University of California, 405 Hilgard Avenue, Los Angeles, CA 90095, USA
J Exp Bot 57:1515-22. 2006.... - Top-down mass spectrometry of integral membrane proteinsJulian Whitelegge
University of California, Pasarow Mass Spectrometry Laboratory, Jane and Terry Semel Institute for Neuroscience and Human Behavior, David Geffen School of Medicine, Los Angeles, CA 90024, USA
Expert Rev Proteomics 3:585-96. 2006..Top down offers a more detail-orientated approach to post-transcriptional and post-translational diversity allowing an enhanced insight beyond genomic translation, which has now extended into the bilayer proteome... - Toward the bilayer proteome, electrospray ionization-mass spectrometry of large, intact transmembrane proteinsJ P Whitelegge
Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095, USA
Proc Natl Acad Sci U S A 96:10695-8. 1999..Furthermore, ESI-MS is used to titrate reactive thiols with N-ethylmaleimide. Treatment of the native protein solubilized in detergent micelles reveals only two reactive thiols, and both are protected by a substrate analog... - Mass spectrometry for high throughput quantitative proteomics in plant research: lessons from thylakoid membranesJulian P Whitelegge
The Pasarow Mass Spectrometry Laboratory, Department of Psychiatry and Biobehavioral Sciences, David Geffen School of Medicine and College of Letters and Sciences, University of California, Los Angeles, CA, USA
Plant Physiol Biochem 42:919-27. 2004.... - Disulfide connectivity in cerebroside sulfate activator is not necessary for biological activity or alpha-helical content but is necessary for trypsin resistance and strong ligand bindingK F Faull
Pasarow Mass Spectrometry Laboratory, UCLA, Los Angeles, California 90095, USA
Arch Biochem Biophys 376:266-74. 2000..Some revision of our thoughts on the importance of the disulfide connectivities in the structure and function of the protein are necessary... - Hydrogen-deuterium exchange signature of porcine cerebroside sulfate activator proteinK F Faull
Department of Psychiatry and Biobehavioral Sciences and the Neuropsychiatric Institute, UCLA, Los Angeles, California 90095, USA
J Mass Spectrom 35:392-401. 2000..The hydrogen-deuterium exchange profile will be a valuable criterion for characterizing mutant forms of CSAct produced by recombinant and synthetic paradigms and also the native and mutant forms of related proteins... - Proteomics on full-length membrane proteins using mass spectrometryJ le Coutre
Howard Hughes Medical Institute, Departments of Physiology and of Microbiology and Molecular Genetics, Molecular Biology Institute, University of California, Los Angeles 90095, USA
Biochemistry 39:4237-42. 2000..Finally, using in-line LC-MS, unknown proteins can be identified from solubilized Escherichia coli membranes without prior purification... - Structure of the asparagine-linked sugar chains of porcine kidney and human urine cerebroside sulfate activator proteinK F Faull
Department of Chemistry and Biochemistry, UCLA, Los Angeles, California, 90095, USA
J Mass Spectrom 35:1416-24. 2000..The procedures developed will be useful for sequencing other oligosaccharides including those from other members of the lipid-binding protein class to which cerebroside sulfate activator belongs. (c) 2000 John Wiley & Sons, Ltd... - Cerebroside sulfate activator protein (Saposin B): chromatographic and electrospray mass spectrometric propertiesK F Faull
Pasarow Mass Spectrometry Laboratory, Department of Psychiatry and Biobehavioral Sciences and the Neuropsychiatric Institute and Department of Chemistry and Biochemistry, UCLA, Los Angeles, California 90095, USA
J Mass Spectrom 34:1040-54. 1999.... - Molecular characterization of Vibrio parahaemolyticus vSGLT: a model for sodium-coupled sugar cotransportersE Turk
Department of Physiology, University of California, Los Angeles 90095 1751, USA
J Biol Chem 275:25711-6. 2000..It is further demonstrated that ESI-MS is a powerful tool for the study of proteomics of membrane transporters... - The chloroplast grana proteome defined by intact mass measurements from liquid chromatography mass spectrometryStephen M Gómez
The Pasarow Mass Spectrometry Laboratory, Department of Psychiatry and Biobehavioral Sciences, The Neuropsychiatric Institute and the Department of Chemistry and Biochemistry, UCLA, Los Angeles, California 90095, USA
Mol Cell Proteomics 1:46-59. 2002..Intact mass proteomics of organellar subfractions and more highly purified protein complexes provides increasingly detailed insights into functional genomics of photosynthetic membranes... - Analysis of enzyme kinetics using electrospray ionization mass spectrometry and multiple reaction monitoring: fucosyltransferase VA J Norris
Crump Institute for Molecular Imaging, Departments of Molecular and Medical Pharmacology, Chemistry and Biochemistry, University of California, Los Angeles, California 90095, USA
Biochemistry 40:3774-9. 2001.... - Transit peptide cleavage sites of integral thylakoid membrane proteinsStephen M Gómez
Pasarow Mass Spectrometry Laboratory, Department of Psychiatry and Biobehavioral Sciences, University of California, Los Angeles, California 90095, USA
Mol Cell Proteomics 2:1068-85. 2003.... - Integrating mass spectrometry into membrane protein drug discoveryAdam B Weinglass
Howard Hughes Medical Institute, Departments of Physiology and Microbiology and Molecular Genetics Molecular Biology Institute, University of California Los Angeles, Los Angeles, CA 90095 1662, USA
Curr Opin Drug Discov Devel 7:589-99. 2004.... - Purification and proteomic analysis of outer membrane vesicles from a clinical isolate of Leptospira interrogans serovar CopenhageniJarlath E Nally
Division of Infectious Diseases, Department of Medicine, University of California, Los Angeles, CA 90095, USA
Proteomics 5:144-52. 2005.... - A monoclonal antibody that conveys in vitro killing and partial protection in experimental syphilis binds a phosphorylcholine surface epitope of Treponema pallidumDavid R Blanco
A2 087G Center for Health Sciences, UCLA School of Medicine, Los Angeles, CA 90095, USA
Infect Immun 73:3083-95. 2005..This is the first physical demonstration of an antigen on the T. pallidum surface and indication that such a surface antigen can be a target of immunity... - Post-translational modifications of integral membrane proteins resolved by top-down Fourier transform mass spectrometry with collisionally activated dissociationChristopher M Ryan
The Pasarow Mass Spectrometry Laboratory, The Neuropsychiatric Institute NPI Semel Institute for Neuroscience and Human Behavior, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California 90024, USA
Mol Cell Proteomics 9:791-803. 2010.... - A novel mass spectrometric assay for the cerebroside sulfate activator protein (saposin B) and arylsulfatase AAndrew J Norris
Pasarow Mass Spectrometry Laboratory, Department of Psychiatry, and Neuropsychiatric Institute, University of California Los Angeles, Los Angeles, CA 90024, USA
J Lipid Res 46:2254-64. 2005..The modified assay for the coupled ASA/CSAct reaction could find applicability in settings in which the assay could not be performed previously because of the need for radiolabeled substrate, which is now not required... - Mass spectral analysis of the apolipoproteins on mouse high density lipoproteins. Detection of post-translational modificationsDonald L Puppione
The Molecular Biology Institute and The Department of Chemistry and Biochemistry, University of California at Los Angeles, Los Angeles, CA 90095, USA
Biochim Biophys Acta 1764:1363-71. 2006.... - Mass spectral analysis of domestic and wild equine apoA-I and A-II: detection of unique dimeric forms of apoA-IIDonald L Puppione
The Molecular Biology Institute and The Department of Chemistry and Biochemistry, University of California at Los Angeles, 90095, USA
Comp Biochem Physiol B Biochem Mol Biol 142:369-73. 2005..In addition, our data indicate that small amounts of apoA-I and apoA-II apparently are acylated... - Temporal analysis of the antigenic composition of Borrelia burgdorferi during infection in rabbit skinTimothy R Crother
Department of Medicine, Division of Infectious Diseases, University of California, Los Angeles, 37 121 Center for Health Sciences, 10833 LeConte Ave, Los Angeles, CA 90095, USA
Infect Immun 72:5063-72. 2004..This marked diminution in VlsE lower forms paralleled the clearance of the spirochete from skin... - Identification of a novel phosphorylation site in c-jun directly targeted in vitro by protein kinase DRichard T Waldron
Unit of Signal Transduction and Gastrointestinal Cancer, Division of Digestive Diseases, Department of Medicine, David Geffen School of Medicine, University of California, Los Angeles, USA
Biochem Biophys Res Commun 356:361-7. 2007..These data were further supported by kinase reactions using truncations or point-mutations of GST-c-jun. Together, these data suggest that PKD-mediated phosphorylation modulates c-jun at the level of its N-terminal functional domains... - Hepatic lipase maturation: a partial proteome of interacting factorsMark H Doolittle
Department of Medicine, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA
J Lipid Res 50:1173-84. 2009..BiP/Grp94 may stabilize misfolded HL during its transition between cycles of CNX binding and may help direct its eventual degradation... - Sequence of horse (Equus caballus) apoA-II. Another example of a dimer forming apolipoproteinDonald L Puppione
Boyer Hall, The Molecular Biology Institute and The Department of Chemistry and Biochemistry, University of California at Los Angeles, Los Angeles, CA 90095, USA
Comp Biochem Physiol B Biochem Mol Biol 138:213-20. 2004..The data are compared with other mammalian sequences of apoA-II and are discussed in terms of resulting similarities and variations in the primary sequences... - Mass spectral analyses of the two major apolipoproteins of great ape high density lipoproteinsDonald L Puppione
The Molecular Biology Institute, UCLA, Los Angeles, CA 90095, USA
Comp Biochem Physiol Part D Genomics Proteomics 4:305-9. 2009..Post-translational modification of these apolipoproteins, involving truncation and oxidation of methionine, are also reported... - Cysteine string protein beta is prominently associated with nerve terminals and secretory organelles in mouse brainCameron B Gundersen
the Department of Molecular and Medical Pharmacology, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA
Brain Res 1332:1-11. 2010..Collectively, the enrichment of CSP-beta in synaptosomes and the association of CSP-beta with synaptic vesicles suggest that CSP-beta, like CSP-alpha, may be an important component of the regulated secretory machinery in mouse brain... - Demonstration by mass spectrometry that purified native Treponema pallidum rare outer membrane protein 1 (Tromp1) has a cleaved signal peptideD R Blanco
Department of Microbiology, Immunology, and Molecular Genetics, University of California at Los Angeles, Los Angeles, California 90095, USA
J Bacteriol 181:5094-8. 1999..These studies confirm that Tromp1 is processed and does not contain an uncleaved signal peptide as previously reported... - Kinetic characterization of enzyme inhibitors using electrospray-ionization mass spectrometry coupled with multiple reaction monitoringA J Norris
Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, UCLA School of Medicine, Los Angeles, California 90095-1770, USA
Anal Chem 73:6024-9. 2001..ESI-MS/MRM is therefore a powerful tool for the kinetic characterization of enzyme inhibitors, providing complete disclosure of the mechanism of action of 1 as an inhibitor... - Characterization of the outer membrane proteome of Leptospira interrogans expressed during acute lethal infectionJarlath E Nally
Division of Infectious Diseases, Department of Medicine, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California, USA
Infect Immun 75:766-73. 2007..Significant amounts of LipL32 are also expressed by Leptospira during acute infection of guinea pigs... - Defining intact protein primary structures from saliva: a step toward the human proteome projectF Halgand
NPI Semel Institute for Neuroscience and Human Behavior, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California 90024, United States
Anal Chem 84:4383-95. 2012..This led us to propose guidelines for an iterative processing workflow of MS and MSMS data sets that allow researchers to completely assign the identity and the structure of a protein... - Proteomics. Making sense of genomic information for drug discoveryJ P Whitelegge
Department of Chemistry and Biochemistry, Pasarow Mass Spectrometry Laboratory, University of California Los Angeles, Los Angeles, California, USA
Am J Pharmacogenomics 1:29-35. 2001.... - Detection of two distinct forms of apoC-I in great apesDonald L Puppione
The Molecular Biology Institute, University of California, Los Angeles, CA 90095, USA
Comp Biochem Physiol Part D Genomics Proteomics 5:73-9. 2010..Genomic variations in chromosome 19 among great apes, baboons and macaques as they relate to both genes for apoC-I and the pseudogene are compared and discussed... - Anthrax lethal toxin induced lysosomal membrane permeabilization and cytosolic cathepsin release is Nlrp1b/Nalp1b-dependentKathleen M Averette
Department of Microbiology, Immunology and Molecular Genetics, University of California Los Angeles, Los Angeles, California, USA
PLoS ONE 4:e7913. 2009..These data reveal a role for lysosomal membrane permeabilization in the cellular response to bacterial pathogens and demonstrate a shared requirement for cytosolic relocalization of cathepsins in pyroptosis and pyronecrosis... - Yeast ribosomal/cytochrome c SET domain methyltransferase subfamily: identification of Rpl23ab methylation sites and recognition motifsTanya R Porras Yakushi
Department of Chemistry and Biochemistry, The Molecular Biology Institute, UCLA, Los Angeles, CA 90095 1569, USA
J Biol Chem 282:12368-76. 2007..Finally, we show the presence of Rkm1 homologs in fungi, plants, and mammals including humans... - Differential association of hemoglobin with proinflammatory high density lipoproteins in atherogenic/hyperlipidemic mice. A novel biomarker of atherosclerosisJunji Watanabe
Atherosclerosis Research Unit, Department of Medicine Cardiology, University of California, Los Angeles, California 90095, USA
J Biol Chem 282:23698-707. 2007..Our data suggest that Hb contributes to the proinflammatory nature of HDL in mouse and human models of atherosclerosis and may serve as a novel biomarker for atherosclerosis... - Similar energetic contributions of packing in the core of membrane and water-soluble proteinsNathan H Joh
Department of Chemistry and Biochemistry, UCLA DOE Center for Genomics and Proteomics, Molecular Biology Institute, University of California, Los Angeles, California 90095, USA
J Am Chem Soc 131:10846-7. 2009..We observed little difference in the packing energetics of water and membrane soluble proteins. Our results imply that other mechanisms are employed to stabilize the structure of membrane proteins... - Mass spectrometry (LC-MS/MS) site-mapping of N-glycosylated membrane proteins for breast cancer biomarkersStephen A Whelan
Revlon UCLA Breast Center, Department of Surgery, Neuropsychiatric Semel Institute for Neuroscience and Human Behavior, David Geffen School of Medicine, Los Angeles, California 90095, USA
J Proteome Res 8:4151-60. 2009..Analyzing the N-glycoproteins from membranes of breast cancer cell lines highlights the usefulness of the procedure for generating a practical set of potential biomarkers... - Identification of differentially expressed proteins in murine embryonic and postnatal cortical neural progenitorsLorelei D Shoemaker
Pasarow Mass Spectrometry Laboratory, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California, United States of America
PLoS ONE 5:e9121. 2010..The study of NSPC would be greatly facilitated by the identification of additional proteins that mediate their function and that would distinguish amongst different progenitor populations... - Novel role for Na,K-ATPase in phosphatidylinositol 3-kinase signaling and suppression of cell motilitySonali P Barwe
Department of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA
Mol Biol Cell 16:1082-94. 2005..Thus, these results demonstrate a new role for Na,K-ATPase in regulating carcinoma cell motility... - Intrinsic membrane association of Drosophila cysteine string proteinsA Mastrogiacomo
Department of Molecular and Medical Pharmacology and Crump Institute for Biological Imaging, UCLA School of Medicine, Los Angeles, CA 90095, USA
FEBS Lett 436:85-91. 1998..Thus, we conclude that under physiological conditions csps are integral membrane proteins of secretory organelles, and that the cysteine string domain plays a vital role in the membrane association of these proteins... - Cardiovascular-related proteins identified in human plasma by the HUPO Plasma Proteome Project pilot phaseBeniam T Berhane
Department of Physiology and Medicine/Division of Cardiology, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA
Proteomics 5:3520-30. 2005.... - Detergent-insoluble aggregates associated with amyotrophic lateral sclerosis in transgenic mice contain primarily full-length, unmodified superoxide dismutase-1Bryan F Shaw
Department of Chemistry and Biochemistry, David Geffen School of Medicine, UCLA, Los Angeles, CA 90095, USA
J Biol Chem 283:8340-50. 2008..The results demonstrate that the principal protein in the high molecular mass aggregates whose appearance correlates with symptoms of the disease is the unmodified, full-length SOD1 polypeptide... - Tumor proteomic profiling predicts the susceptibility of breast cancer to chemotherapyJianbo He
Department of Surgery, Gonda UCLA Breast Cancer Research Laboratory, University of California at Los Angeles, Los Angeles, CA 90095, USA
Int J Oncol 35:683-92. 2009..9% of the tumors with pathologically complete response, and 91.7% of the resistant tumors. The data suggest that breast cancer protein biomarkers may be used to pre-select patients for optimal chemotherapeutic treatment... - Rpe65 isomerase associates with membranes through an electrostatic interaction with acidic phospholipid headgroupsQuan Yuan
Jules Stein Eye Institute, UCLA School of Medicine, Los Angeles, California 90095, USA
J Biol Chem 285:988-99. 2010..The interaction of Rpe65 with acidic phospholipids is therefore biologically relevant... - Proteomics of membrane proteinsJulian P Whitelegge
Pasarow Mass Spectrometry Laboratory, Department of Psychiatry and Biobehavioral Sciences, Department of Chemistry and Biochemistry, Neuropsychiatric Institute, University of California Los Angeles, Los Angeles, California 90095, USA
Adv Protein Chem 65:271-307. 2003 - Micro-heterogeneity of human saliva Peptide P-C characterized by high-resolution top-down Fourier-transform mass spectrometryFrederic Halgand
The Pasarow Mass Spectrometry Laboratory, NPI Semel Institute for Neuroscience and Human Behavior, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California, USA
J Am Soc Mass Spectrom 21:868-77. 2010..While the biological significance of such subtle variations in protein structure remains unclear, their importance cannot be assigned without their characterization, as is reported here for one of the major salivary proteins... - Interactions between partner switcher orthologs BtrW and BtrV regulate type III secretion in BordetellaNatalia A Kozak
Department of Microbiology, Immunology and Molecular Genetics, David Geffen School of Medicine at UCLA, 10833 Le Conte Ave, Los Angeles, CA 90095 1747, USA
J Bacteriol 187:5665-76. 2005.... - Confident assignment of intact mass tags to human salivary cystatins using top-down Fourier-transform ion cyclotron resonance mass spectrometryChristopher M Ryan
The Pasarow Mass Spectrometry Laboratory, The NPI Semel Institute for Neuroscience and Human Behavior, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California 90024, USA
J Am Soc Mass Spectrom 21:908-17. 2010..With fully characterized cystatin intact mass tags it will now be possible to examine the correlation between the abundance of these molecules and human health and disease... - Characterization of serum biomarkers for detection of early stage ovarian cancerKatherine R Kozak
Department of Obstetrics and Gynecology, University of California, Los Angeles, USA
Proteomics 5:4589-96. 2005..955). We conclude that TTR, Hb, ApoAI and TF, when combined with CA125 should significantly improve the detection of early stage ovarian cancer... - Local unfolding in a destabilized, pathogenic variant of superoxide dismutase 1 observed with H/D exchange and mass spectrometryBryan Francis Shaw
Department of Chemistry and Biochemistry, University of California Los Angeles, Los Angeles, CA 90095, USA
J Biol Chem 281:18167-76. 2006..These local and global unfolding events could facilitate intermolecular protein-protein interactions that cause the aggregation or neurotoxicity of A4V SOD1... - Modest stabilization by most hydrogen-bonded side-chain interactions in membrane proteinsNathan Hyunjoong Joh
Department of Chemistry and Biochemistry, UCLA DOE Center for Genomics and Proteomics, Molecular Biology Institute, USA
Nature 453:1266-70. 2008..Weak hydrogen-bonding should be reflected in considerations of membrane protein folding, dynamics, design, evolution and function... - Elucidation of substrate binding interactions in a membrane transport protein by mass spectrometryAdam B Weinglass
Department of Physiology, Molecular Biology Institute, University of California Los Angeles, Los Angeles, CA 90095 1662, USA
EMBO J 22:1467-77. 2003..The approach demonstrates that mass spectrometry can provide a powerful means of analyzing ligand interactions with integral membrane proteins... - Identification of the molecular chaperone alpha B-crystallin in demineralized bone powder and osteoblast-like cellsKeyvan Behnam
Department of Physiological Sciences, University of California, Los Angeles, Los Angeles, CA 90024, USA
J Orthop Res 20:1190-6. 2002.... - Side-chain contributions to membrane protein structure and stabilitySalem Faham
Department of Chemistry and Biochemistry, UCLA DOE Center for Genomics and Proteomics, Molecular Biology Institute, University of California, Los Angeles, CA 90095 1570, USA
J Mol Biol 335:297-305. 2004..4) We find little energetic difference, on average, in the burial of apolar surface or polar surface area, implying that van der Waals packing is the dominant force that drives membrane protein folding... - Identification of protein N-terminal methyltransferases in yeast and humansKristofor J Webb
Department of Chemistry and Biochemistry and Molecular Biology Institute, University of California, Los Angeles, 607 Charles E Young Drive East, Los Angeles, California 90095, USA
Biochemistry 49:5225-35. 2010..We conclude that protein X-Pro-Lys N-terminal methylation reactions catalyzed by the enzymes described here may be widespread in nature... - The proteomes of human parotid and submandibular/sublingual gland salivas collected as the ductal secretionsPaul Denny
School of Dentistry, University of Southern California, Los Angeles, California 90089, USA
J Proteome Res 7:1994-2006. 2008.... - Metal-free superoxide dismutase-1 and three different amyotrophic lateral sclerosis variants share a similar partially unfolded beta-barrel at physiological temperatureArmando Durazo
Department of Chemistry and Biochemistry, UCLA, Los Angeles, California 90095, USA
J Biol Chem 284:34382-9. 2009..e. beta3 and beta4, residues 21-53). Together, these results show that human wild-type apo-SOD1 and variants have a partially unfolded beta-barrel at physiological temperature and unfold non-cooperatively... - The murine cardiac 26S proteasome: an organelle awaiting explorationAldrin V Gomes
Department of Physiology and Medicine, and Cardiac Proteomics and Signaling Lab at the Cardiovascular Research Laboratory, University of California at Los Angeles, School of Medicine, Los Angeles, California 90095, USA
Ann N Y Acad Sci 1047:197-207. 2005..In addition, this review shares the limited information that has been garnered thus far pertaining to the molecular composition, function, and regulation of this important organelle in the cardiac cells... - Exercise affects energy metabolism and neural plasticity-related proteins in the hippocampus as revealed by proteomic analysisQinxue Ding
Department of Neurosurgery, Brain Injury Research Centre, UCLA, 621 Charles E. Young Drive, Los Angeles, CA 90095, USA
Eur J Neurosci 24:1265-76. 2006.... - A novel SET domain methyltransferase in yeast: Rkm2-dependent trimethylation of ribosomal protein L12ab at lysine 10Tanya R Porras-Yakushi
Department of Chemistry and Biochemistry, Molecular Biology Institute, UCLA, Los Angeles, California 90095 1569, USA
J Biol Chem 281:35835-45. 2006..These studies suggest that the remaining seven SET domain proteins may also be lysine methyltransferases... - A new endogenous form of PYY isolated from canine ileum: Gly-extended PYY(1-36)David A Keire
CURE Digestive Diseases Research Center, VA GLAHS, Los Angeles, CA 90073 and Digestive Diseases Division, David Geffen School of Medicine at UCLA, Los Angeles, CA 90024, United States
Regul Pept 151:61-70. 2008..In addition, we also found that the PYY(3-36)-NH(2) form represents 6% of the total PYY in the canine ileo-cecal junction. The physiological implication of the Gly-extended form of PYY(1-36) warrants further investigation... - A novel SET domain methyltransferase modifies ribosomal protein Rpl23ab in yeastTanya R Porras-Yakushi
Department of Chemistry and Biochemistry and the Molecular Biology Institute, The Pasarow Mass Spectrometry Laboratory, University of California, Los Angeles, California 90095 1569, USA
J Biol Chem 280:34590-8. 2005..These results show that SET domain methyltransferases can be involved in translational roles as well as in the previously described transcriptional roles... - Detection of breast cancer biomarkers in nipple aspirate fluid by SELDI-TOF and their identification by combined liquid chromatography-tandem mass spectrometryJianbo He
Gonda UCLA Breast Cancer Research Laboratory, David Geffen School of Medicine, Los Angeles, CA 90095, USA
Int J Oncol 30:145-54. 2007.... - A two component chitin-binding protein from French bean -- association of a proline-rich protein with a cysteine-rich polypeptideLaurence V Bindschedler
School of Biological Sciences, Royal Holloway, University of London, Egham Hill, Egham, Surrey TW20 0EX, UK
FEBS Lett 580:1541-6. 2006.... - Exploring the role of a unique carboxyl residue in EmrE by mass spectrometryAdam B Weinglass
Howard Hughes Medical Institute, Department of Physiology, Molecular Biology Institute, University of California Los Angeles, 90095 1662, USA
J Biol Chem 280:7487-92. 2005..Taken together with other biochemical data, the findings support a "time sharing" mechanism in which both Glu-14 residues in a dimer are involved in tetraphenylphosphonium and H(+) binding... - J-domain protein CDJ2 and HSP70B are a plastidic chaperone pair that interacts with vesicle-inducing protein in plastids 1Cuimin Liu
Plant Biochemistry, Institute of Biology II, University of Freiburg, D-79104 Freiburg, Germany
Mol Biol Cell 16:1165-77. 2005..Our findings and the previously reported requirement of VIPP1 for the biogenesis of thylakoid membranes point to a role for the HSP70B/CDJ2 chaperone pair in this process... - Crystal structure of saposin B reveals a dimeric shell for lipid bindingVictoria E Ahn
Department of Medical Biophysics, University of Toronto, ON, Canada M5G 2M9
Proc Natl Acad Sci U S A 100:38-43. 2003..A comparison of the two forms of the saposin B dimer suggests that extraction of target lipids from membranes involves a conformational change that facilitates access to the inner cavity... - Monitoring conformational rearrangements in the substrate-binding site of a membrane transport protein by mass spectrometryAdam Weinglass
Howard Hughes Medical Institute and Department of Physiology and Microbiology, University of California, Los Angeles, Los Angeles, CA 90095, USA
J Biol Chem 279:41858-65. 2004.... - Mapping the cofilin binding site on yeast G-actin by chemical cross-linkingElena E Grintsevich
Department of Chemistry and Biochemistry, University of California Los Angeles, CA, USA
J Mol Biol 377:395-409. 2008..4 A) and via disulfide bond formation was also documented. This set of cross-linking data confirms the important role of the N-terminal segment of cofilin in interactions with G-actin... - Amide bonds assemble pili on the surface of bacilliJonathan M Budzik
Department of Microbiology, University of Chicago, Chicago, IL 60637, USA
Proc Natl Acad Sci U S A 105:10215-20. 2008..Conservation of catalysts and structural elements in pilin precursors in Gram-positive bacteria suggests a universal mechanism of fiber assembly... - Proteomic profiling of cancer biomarkersHungyi Shau
Revlon/UCLA Breast Center, 90095-7028, USA
Brief Funct Genomic Proteomic 2:147-58. 2003..The aim is to provide a resource for the experimental practitioner seeking awareness of the analytical tools that are now available in contemporary cancer research... - Rhizobium common nod genes are required for biofilm formationNancy A Fujishige
Department of Molecular, Cell and Developmental Biology, University of California Los Angeles, CA 90095 1606, USA
Mol Microbiol 67:504-15. 2008.... - Inhibition kinetics of carba- and C-fucosyl analogues of GDP-fucose against fucosyltransferase V: implication for the reaction mechanismAndrew J Norris
Department of Molecular and Medical Pharmacology, David Geffen School of Medicine at University of California, Los Angeles, CA 90095, USA
Bioorg Med Chem Lett 14:571-3. 2004..1+/-9.8 microM, similar to the K(m) value for GDP-Fuc (50.4+/-5.5 microM), while the C-Fuc analogue exhibited significantly weak competitive inhibition with a K(i) value of 889+/-93 microM... - The minimum information about a proteomics experiment (MIAPE)Chris F Taylor
The HUPO Proteomics Standards Initiative, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire CB10 1SD, UK
Nat Biotechnol 25:887-93. 2007.... - Increased coverage in the transmembrane domain with activated-ion electron capture dissociation for top-down Fourier-transform mass spectrometry of integral membrane proteinsVlad Zabrouskov
Thermo Fisher, San Jose, California, California 95134, USA
J Proteome Res 6:2205-10. 2007..Thermal activation concomitant with electron delivery was necessary for efficient ECD (activated-ion ECD; aiECD), yielding complementary information and greater sequence coverage in the transmembrane domains in comparison with CAD... - Metal-free superoxide dismutase forms soluble oligomers under physiological conditions: a possible general mechanism for familial ALSLucia Banci
Magnetic Resonance Center CERM and Department of Chemistry, University of Florence, Italy
Proc Natl Acad Sci U S A 104:11263-7. 2007..Although we cannot exclude other mechanisms in SOD1-linked familial ALS, the one proposed here has the strength of explaining how a large and diverse set of SOD1 mutant proteins all could lead to disease through the same mechanism... - The evolution of transmembrane helix kinks and the structural diversity of G protein-coupled receptorsSarah Yohannan
Department of Chemistry and Biochemistry, UCLA DOE Institute for Genomics and Proteomics, Molecular Biology Institute, University of California, Los Angeles, CA 90095, USA
Proc Natl Acad Sci U S A 101:959-63. 2004..Our analysis allows us to predict kink positions with >90% reliability. Kink prediction indicates that different G protein-coupled receptor proteins have different kink patterns and therefore different structures...